JP2010060571A5 - - Google Patents

Claims (15)

In order to suppress a decrease in measured value due to an interfering substance in a test sample , which is a polymer obtained by polymerizing an aromatic monomer having a sulfone group or a salt thereof and having a molecular weight of 1,000 to 100,000. Measurement value decrease inhibitor for immunoassay. The measurement according to claim 1, wherein the polymer is a polymer obtained by polymerizing an aromatic monomer having a sulfone group or a salt thereof , in which the repeating unit is represented by the following general formula [I] or the following general formula [V]. Value decrease inhibitor.

(Wherein Ar represents an aromatic ring, X represents a sulfone group or a salt thereof, R ¹ to R ³ each independently represents a hydrogen atom or a lower alkyl group, n represents an integer of 0 to 10, and each carbon constituting Ar The hydrogen atom bonded to the atom may be substituted with a lower alkyl group or a lower alkoxy group ).

(However, M represents an atom or group that becomes a monovalent cation in an aqueous solution). The repeating units represented by the general formula [I], the measured values decrease inhibitor according to claim 2, wherein the aromatic ring is a benzene ring. The measured value decrease inhibitor according to claim 3, wherein the repeating unit is represented by the following general formula [II].

(Wherein, M is a monovalent cation in aqueous solution atom or group, R ¹ to R ³ are R ¹ to in the above general formula [I] R ³ as defined, R ⁴ to R ^6, independently of one another , Represents a hydrogen atom, a lower alkoxyl group or a lower alkyl group) The measured value reduction inhibitor according to claim 4 , wherein the repeating unit is anethole sulfonate or styrene sulfonate. The measured value decrease inhibitor according to any one of claims 1 to 5 , wherein the immunoassay is an immunoagglutination method. An immunoassay performed in the presence of the measured value decrease inhibitor according to any one of claims 1 to 6 ( except when it is performed on an undiluted test sample) . A first step of contacting a test sample and the measured value decreased inhibitor, then, according to claim 7 further comprising a second step of sensitizing the particles or antiserum and antigen-antibody reaction test sample after the first step Immunoassay. The immunoassay method according to claim 7 or 8 , wherein the test sample is a biological sample. The immunoassay method according to claim 9 , wherein the test sample is blood, serum or plasma. The immunoassay method according to any one of claims 7 to 10 , wherein the concentration of the measured value decrease inhibitor in the reaction solution is 0.01% to 5% (weight / volume). The immunoassay method according to any one of claims 7 to 11, which is an immunoagglutination method. A reagent for immunoassay comprising at least a buffer solution and sensitizing particles or antiserum, comprising the measured value decrease inhibitor according to any one of claims 1 to 6 , . The immunoassay reagent includes at least a buffer solution, and includes at least a first reagent mixed with the test sample, a buffer solution and sensitized particles, and is added to the mixture of the test sample and the first reagent. that a two-part system reagent and a second reagent, the measurement value lowering inhibitor, a reagent of claim 1 3, wherein included in said first reagent. Reagent according to claim 1 3 or 1 4, wherein said immunoassay is an immunoagglutination method.