JP2010019579A - Transilluminator for migration gel - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a transilluminator for a migration gel, sharply reducing the damage caused by the irradiation with the exciting light of nucleic acid in an electrophoretic gel, sufficiently ensuring the cutting-off work time of a region where the band of the gel is positioned, and enhancing the certainly of cutting-off work. <P>SOLUTION: The transilluminator 10 for the migration gel has a light source 30 which outputs exciting light into a housing 20 having a light transmitting plate 21 forming a gel installing surface 21a, built therein, and is constituted so that a shading sheet 40 for opening and closing the light transmitting plate 21 and the light source 30 is housed in the housing 20, and that a movable member 50 for on-off operation is attached to the shading sheet 40. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a transilluminator for electrophoresis gel that irradiates an electrophoresis gel of nucleic acid or protein with excitation light that excites a fluorescent staining agent that stains the nucleic acid or protein molecule in the gel to generate fluorescence.

As a method for analyzing nucleic acids such as DNA and RNA, the nucleic acids are separated by electrophoresis in a gel such as agarose gel or polyacrylimide gel (eg DGGE method: DGGE: Denaturing Gradient Gel Electrophoresis) However, a method of observing this by a fluorescence method is widely used.
When a sample containing a nucleic acid is electrophoresed in a gel, the nucleic acid is separated by a difference in moving speed according to a difference in molecular weight or the like. The nucleic acid in the gel is stained with a fluorescent stain that binds to the nucleic acid molecule (hereinafter also referred to simply as a stain; for example, ethidium bromide). The stain is added to the gel at the time of preparation of the gel, or the sample to which the stain solution is added is placed on the gel for electrophoresis, or the sample is immersed in the stain solution after electrophoresis of the sample. In such a manner, it is mixed in the gel and bound to the nucleic acid molecule. Excess stain is washed away.

  As the staining agent, those that emit fluorescence using ultraviolet light as excitation light are widely used (for example, Patent Documents 1 and 2). When such a stain is used, when the gel after electrophoresis is irradiated with ultraviolet rays, the stain bound to the nucleic acid emits fluorescence, so that the position of the nucleic acid separated by electrophoresis becomes visible. . The movement speed of the nucleic acid contained in the sample is determined by the molecular weight, size, and the like. And the nucleic acid in a sample forms the band according to the moving speed in the case of electrophoresis. A portion containing a desired band in the gel can be cut out and subjected to PCR analysis (PCR: Polymerase Chain Reaction).

Here, an irradiation device that irradiates the gel with excitation light of a staining agent is referred to as a transilluminator. As this transilluminator, those equipped with an ultraviolet lamp for irradiating ultraviolet rays are widely used.
Japanese Unexamined Patent Publication No. 7-190934 JP 2003-194721 A

In the case of the above analysis method, there is a measure of using a transilluminator equipped with an ultraviolet lamp having a longer wavelength (for example, 300 nm or more) in order to reduce damage (damage) of nucleic acid molecules due to ultraviolet irradiation. However, in order to reduce damage to nucleic acid molecules as much as possible, it is necessary to shorten the time during which the nucleic acid is exposed to ultraviolet rays, and it is necessary to cut out the band from the gel as quickly as possible. .
In addition, cutting out the bands from the gel is performed manually one band at a time, so there is a limit to shortening the working time. Transilluminators are generally configured to irradiate the entire gel with UV light when the UV lamp is turned on, so when there are many samples, the longer the time of cutting, the longer it will be exposed to UV light. Nucleic acid molecules are severely damaged, and as a result, there is a problem that the success rate of PCR analysis is lowered due to nucleic acid damage.

  In recent years, transilluminators that use visible light as excitation light have also been developed and put into practical use. However, if the irradiation time of excitation light is long, it may affect the molecular structure of nucleic acids. . For this reason, even when light in the visible light region is used as excitation light for a staining agent, there is a demand for suppressing the influence of irradiation with excitation light.

  In view of the above problems, the present invention can greatly reduce nucleic acid damage (damage) due to excitation light irradiation with a simple configuration, and also has a sufficient working time for cutting out a site where a gel band is located. An object of the present invention is to provide a transilluminator for electrophoresis gel that can be secured and can improve the reliability of cutting work.

In order to solve the above problems, the present invention provides the following configuration.
A first invention is an electrophoretic gel transilluminator that irradiates a nucleic acid or protein electrophoresis gel with excitation light that excites a fluorescent staining agent that stains the nucleic acid or protein molecule in the gel to generate fluorescence. A housing, a light source that is housed in the housing and outputs the excitation light, a light transmitting plate that forms part of the housing and transmits the excitation light, and the light source. A light-shielding sheet interposed in the space, and attached to an end of the light-shielding sheet opposite to a base end attached to the casing, and in a direction perpendicular to the light-transmitting plate in the casing A movable member that moves in the longitudinal direction of a long hole that extends along the translucent plate in a side wall provided so as to surround the space between the translucent plate and the light source. And the inner space of the casing of the translucent plate The electrophoresis gel is installed on the gel installation surface which is the opposite surface, and the movable member has an operation handle portion which protrudes to the outside of the casing through the elongated hole. The base end portion of the light shielding sheet is provided at a position avoiding the space between the light source and the translucent plate in the longitudinal direction of the elongated hole, and the movable member is moved to move the movable light member from the light source. A transilluminator for electrophoretic gel, characterized in that an irradiation range of the excitation light to a light transmitting plate is changed.
2nd invention is the 1st light shielding sheet which is the said light shielding sheet which has the said base end part in one of both sides through the said space between the said light source and the said light transmission board in the longitudinal direction of the said long hole, The first movable member, which is the movable member attached to the end of the first light shielding sheet opposite to the base end, and the space between the light source and the translucent plate, The second light-shielding sheet, which is the light-shielding sheet having the base end on the side opposite to the first light-shielding sheet, and the end attached to the end of the second light-shielding sheet on the side opposite to the base end A second movable member that is a movable member, and the operation handle portion of the first movable member and the operation handle portion of the second movable member protrude outside the casing through the elongated hole. A transillumination gel for electrophoresis gel according to the first aspect of the invention To provide over data.
According to a third aspect of the present invention, there is further provided a second elongated hole formed in the side wall portion of the casing so as to extend along the translucent plate in a direction orthogonal to the longitudinal direction of the elongated hole, and the second elongated hole. An auxiliary light-shielding sheet that is the light-shielding sheet attached to the housing at a position avoiding the space between the light source and the translucent plate in the extending direction of the hole, and the auxiliary light-shielding sheet And an auxiliary movable member that is the movable member that is attached to the end opposite to the base end attached to the housing and is moved along the extending direction of the second elongated hole. The transilluminator for electrophoretic gels of the first or second invention is provided.
According to a fourth aspect of the present invention, the movable member is a rod-shaped portion provided so as to span the side wall portions located on both sides through a space secured between the light transmitting plate and the light source in the housing. And the operation handle portion protruding from the rod-shaped portion, and a guide portion for guiding the movement of the rod-shaped portion of the movable member is provided on the side wall portion of the housing. A transilluminator for electrophoretic gel according to any one of the first to third aspects of the invention is provided.
According to a fifth aspect of the invention, the housing includes a light shielding sheet storage unit for storing the light shielding sheet between the space between the light source and the light transmitting plate and a base end portion of the light shielding sheet. The transilluminator for electrophoretic gels according to any one of the first to fourth inventions is provided.
A sixth invention provides the transilluminator for electrophoretic gel according to any one of the first to fifth inventions, wherein the light-shielding sheet is bent into a corrugated shape and can be freely extended and folded. To do.

  According to the transilluminator for electrophoretic gel according to the present invention, the electrophoretic gel transilluminator is provided with an openable / closable light-shielding sheet that can be drawn into a space between the light-transmitting plate and the light source, and between the light source and the light-transmitting plate. By interposing the light shielding sheet, the excitation light output from the light source can be shielded by the light shielding sheet so as not to reach the translucent plate and the electrophoresis gel installed on the gel installation surface of the translucent plate. Further, by moving the movable member by operating the operation handle protruding outside the casing, the excitation light output from the light source is shielded by the light shielding sheet, and the excitation from the light source to the light transmitting plate The light irradiation range can be changed.

In the area where the excitation light output from the light source is shielded by the light shielding sheet (light-shielding area), the nucleic acid or protein (hereinafter, the nucleic acid and protein may be collectively referred to as the analyte) may be damaged by the irradiation of the excitation light. (Damage) can be prevented.
Operations such as grasping the band obtained by electrophoresis and cutting out the portion where the gel band is located are performed in the region (irradiation region) where the light transmitted from the light source is irradiated onto the light transmitting plate. An irradiation area | region is limited to the area | region which performs work, such as cutting out the site | part corresponding to a band in a gel. Thereby, irradiation of the excitation light to the nucleic acid before the cutting operation can be suppressed in a short time. Therefore, the damage (damage) of the analysis target substance due to the irradiation of excitation light can be greatly reduced. As a result, it becomes possible to secure a sufficient work time for cutting out the part where the gel band is located, so that it is possible to reduce the case of failing to cut out due to work, and to improve the work certainty of cutting. There are also advantages.

  Hereinafter, a transilluminator for electrophoretic gel (hereinafter sometimes abbreviated as transilluminator) embodying the present invention will be described with reference to the drawings.

(First embodiment)
First, the transilluminator according to the first embodiment of the present invention will be described.
1 is a front view showing the transilluminator 10, FIG. 2 is a perspective view showing the transilluminator 10, FIG. 3 is a cross-sectional view taken along the line AA in FIG. 1, and FIGS. Irradiation of excitation light in the translucent plate 21 and the electrophoresis gel G (also simply referred to as gel) installed on the gel installation surface 21a of the translucent plate 21 by changing the position of the movable end 42 of the light shielding sheet 40 provided in It is a figure explaining the change of a range.
In FIGS. 1 to 4A to 4C, the upper side is described as the upper side and the lower side as the lower side.

  As shown in FIGS. 1 to 4 (a) to 4 (c), the transilluminator 10 stains the nucleic acid or protein molecule in the gel G on the gel G on which the sample containing the nucleic acid or protein is electrophoresed. Excitation light that excites a fluorescent stain to generate fluorescence, and illuminates a casing 20, a light source 30 that is housed in the casing 20 and outputs the excitation light, and a part of the casing 20 A light shielding sheet 40 interposed in a space 22 (hereinafter also referred to as a sheet drawing space) secured between the light transmitting plate 21 and the light source 30, and the light shielding sheet 40 is attached to the housing 20 And a movable member 50 attached to an end portion (movable end 42) opposite to the base end portion 41.

In the following, an example using an electrophoresis gel (electrophoresed gel) from which nucleic acid molecules have been separated by electrophoresis will be described as gel G. Instead, protein molecules are separated by electrophoresis as gel G. Needless to say, it is also possible to use a gel obtained.
Formation of a band corresponding to the moving speed by electrophoresis in a gel is the same as in the case of gel subjected to electrophoresis of nucleic acid in the case of gel subjected to protein electrophoresis.

The casing 20 is formed in a box shape, and includes a bottom plate 23 (see FIGS. 3 and 4A to 4C), and four side wall portions 24 erected on the bottom plate 23 in a square frame shape. The side wall portion 24 provided in a square frame shape is provided with a gel installation plate 25 that closes the side opposite to the bottom plate 23.
The gel installation plate 25 is obtained by fixing the translucent plate 21 to the opening 25b inside the square frame-shaped frame plate 25a. The gel installation plate 25 is provided by fixing a square frame-like frame plate 25a to an end portion of the side wall portion 24 provided in a square frame shape on the side opposite to the bottom plate 23, and is provided in a square frame shape. It arrange | positions so that the side opposite to the said baseplate 23 of the said side wall part 24 may be plugged up. The gel installation plate 25 is parallel to the bottom plate 23.

The surface opposite to the surface (back surface 21b) facing the inner space 26 of the housing 20 of the translucent plate 21 is a gel installation surface 21a that is a flat surface on which the electrophoresis gel G formed in a sheet shape is installed. It is said that.
In the case 20 of the illustrated example, the translucent plate 21 is formed in a square plate shape whose outer periphery coincides with the inner periphery of the frame plate 25 a of the gel installation plate 25. Moreover, in the housing | casing 20, the frame board 25a of the gel installation board 25 is provided so that it may protrude in the inner peripheral side from the said side wall part 24 provided in square frame shape, and the translucent board 21 is the back surface. The whole 21 b is arranged so as to face the inner space 26 of the housing 20.

The four side wall portions 24 of the housing 20 are provided in a direction perpendicular to the translucent plate 21, and are arranged so as to surround the sheet drawing space 22 in the housing 20.
An elongated hole 27 for guiding the movement of the movable member 50 is formed in one of the four side wall portions 24. The elongated hole 27 is formed so as to extend along the translucent plate 21.

In addition, about this transilluminator 10, the direction along the longitudinal direction of the long hole 27 is hereinafter referred to as the horizontal direction, the direction perpendicular to the horizontal direction and along the translucent plate 21 (specifically, the gel installation surface 21a), It may be described as a vertical direction.
Of the four side wall portions 24 of the housing 20, the operation side wall portion 241 which is the side wall portion 24 in which the elongated hole 27 is formed and the side wall portion facing the operation side wall portion 241 via the inner space 26 of the housing 20. The rear wall portion 244, which is 24, has a long plate shape extending in the lateral direction. Two side wall parts 24 (end walls 242 and 243) other than the operation side wall part 241 and the back wall part 244 are perpendicular to the operation side wall part 241 and the back wall part 244.
Further, the square plate-like light transmitting plate 21 is provided in such a direction that two of the four sides of the outer periphery are in the horizontal direction and the other two sides are in the vertical direction.

The light source 30 outputs excitation light that excites a staining agent (fluorescent staining agent) bound to a nucleic acid molecule in the electrophoresis gel G to generate fluorescence. As is well known, stains generally have individual excitation wavelengths suitable for emitting fluorescence. The light source 30 outputs light having a wavelength corresponding to the excitation wavelength of the stain as excitation light.
The light source 30 is, for example, an ultraviolet lamp that outputs ultraviolet light as excitation light, but may be a lamp that outputs visible light.
In addition, as the translucent plate 21, what transmits the light of the excitation wavelength which the light source 30 outputs is employ | adopted.

  As shown in FIGS. 3 and 4A to 4C, the light source 30 is formed in a cylindrical tubular shape, and a plurality of locations (a plurality of locations in the lateral direction) along the longitudinal direction of the elongated hole 27 in the housing 20. ), Each extending vertically. However, the light source 30 only needs to be able to irradiate the entire light-transmitting plate 21 directly or via a reflecting plate provided in the housing 20, and is limited to a cylindrical tube. For example, a surface emitting type can also be used.

The transilluminator 10 will be described as being used in a direction (hereinafter, in a horizontally placed state) in which the bottom plate 23 side of the housing 20 is down and the gel installation plate 25 side is up.
The electrophoresis gel G is installed so as to be superposed on the gel installation surface 21a formed by the translucent plate 21. In the transilluminator 10, the excitation light output from the light source 30 is transmitted through the translucent plate 21, and is applied to the electrophoresis gel G installed on the gel installation surface 21a.

1 and 2, reference numeral 31 denotes a switch for switching on / off the power source of the light source 30.
In FIG. 1 and FIG. 2, the switch 31 is provided on the operation side wall portion 241 which is the side wall portion 24 in which the long hole 27 is formed, among the four side wall portions 24 of the housing 20. The installation position of the switch 31 may be the side wall part 24 other than the operation side wall part 241. The switch 31 may be attached to a cable extended from the housing 20 and installed at a position away from the housing 20.

The illustrated transilluminator 10 is provided with two light shielding sheets 40 and two movable members 50.
Each of the light shielding sheets 40 has a pair of side wall portions 24 (end wall 242, end wall 242, adjacent to the operation side wall portion 241 of the four side wall portions 24 of the housing 20 at one end in the longitudinal direction (base end portion 41). 243). 4A to 4C, reference numeral 24a denotes a fixing component for fixing one end (base end portion 41) in the longitudinal direction of the light shielding sheet 40 to the housing 20 (specifically, an end wall). .

The transilluminator 10 is provided with two light shielding units 60 (a first light shielding unit 60A and a second light shielding unit 60B) in which the movable member 50 is attached to the movable end 42 of the light shielding sheet 40.
One of the two light shielding units 60 (first light shielding unit 60A) is configured such that one end in the longitudinal direction (base end portion 41) of the light shielding sheet 40 (hereinafter also referred to as the first light shielding sheet 40A) is a pair of end walls of the housing 20. It is attached to one of 242 and 243 (here, the end wall of reference numeral 242). The light shielding sheet 40 (hereinafter also referred to as the second light shielding sheet 40B) of the other light shielding unit 60 (second light shielding unit 60B) has one end (base end portion 41) in the longitudinal direction of the pair of end walls 242 and 243. It is attached to the other (here, the end wall denoted by reference numeral 243).

In addition, the movable member 50 provided in each of the two light shielding units 60 (the first light shielding unit 60A and the second light shielding unit 60B) of the transilluminator 10 is formed on the operation side wall 241. It is guided by one long hole 27 and is movable along the longitudinal direction of the long hole 27 (movable in the lateral direction).
The light shielding unit 60 including the first light shielding sheet 40A and the movable member 50 (first movable member 50A) attached to the movable end 42 of the first light shielding sheet 40A includes the first light shielding unit 60A and the second light shielding sheet 40B. The light shielding unit 60 including the movable member 50 (second movable member 50B) attached to the movable end 42 of the two light shielding sheet 40B is the second light shielding unit 60B.

  The movable member 50 includes a rod-shaped portion 51 provided so as to cross the inner space 26 of the housing 20 in a direction perpendicular to the operation side wall portion 241 (that is, a direction extending in the vertical direction), and the rod-shaped portion. 51, an operation handle portion 52 that protrudes to the outside of the housing 20 from an end portion (hereinafter also referred to as an operation side end portion, reference numeral 51a) inserted into the elongated hole 27 of the operation side wall portion 241. Yes. The rod-like portion 51 of the movable member 50 is provided so as to be bridged over the side wall portions 241 and 244 located on both sides via the sheet drawing space 22.

  As shown in FIG. 2, FIG. 3, etc., the end of the rod-like portion 51 of the movable member 50 opposite to the operation side end 51 a (hereinafter also referred to as the back side end 51 b) A guide groove 28 formed in parallel with the elongated hole 27 of the operation side wall 241 is inserted into the back wall 244, which is the side wall 24 facing the operation side wall 241 through the space 26. In the movable member 50, both ends of the rod-like portion 51 are guided by the elongated hole 27 and the guide groove 28, and are movable in the longitudinal direction of the elongated hole 27 and the longitudinal direction of the guide groove 28.

  As shown in FIGS. 5 and 6, the light shielding sheet 40 is formed with a mountain fold portion 43 a and a valley fold portion 43 b on a belt-like flexible sheet member that can shield the excitation light output from the light source 30. Is. A plurality of mountain fold portions 43a and valley fold portions 43b are formed so as to be alternately arranged in the longitudinal direction of the sheet-like member. The mountain fold portion 43a and the valley fold portion 43b are formed in a linear shape parallel to each other in a direction perpendicular to the longitudinal direction of the sheet-like member. The light shielding sheet 40 is housed in the housing 20 in such a direction that the mountain folds 43a and the valley folds 43b extend in the vertical direction.

Hereinafter, the mountain fold 43 a and the valley fold 43 b are collectively referred to as a bent portion 43.
As shown in FIGS. 4A to 4C, the light shielding sheet 40 is movable from a base end portion 41 attached to the housing 20 between the gel installation plate 25 and the bottom plate 23 of the housing 20. It is provided so as to extend over the member 50. The movable member 50 is provided at a position spaced apart from the base end portion 41 in the lateral direction (the direction along the longitudinal direction of the long hole 27).

The light shielding sheet 40 extends in the lateral direction from the base end portion 41 to the movable member 50 in the housing 20.
Among the bent portions 43 of the light shielding sheet 40, the mountain fold portions 43 a indicate those convex toward the gel installation plate 25 side (upper side in FIG. 5) of the housing 20. Contrary to the mountain fold 43a, the valley fold 43b refers to one that is convex (concave with respect to the gel installation plate 25) on the bottom plate 23 side (lower side in FIG. 5) of the housing 20.

The sheet-shaped member constituting the light-shielding sheet 40 is, for example, an integrally molded product made of synthetic resin. In addition to this, a synthetic resin material is applied to a woven or non-woven fabric, or a woven or non-woven fabric formed of synthetic fibers or natural fibers. An impregnated or coated one can also be used.
Examples of the light shielding sheet 40 that shields ultraviolet rays emitted from the light source 30 include a synthetic resin material mixed with an ultraviolet absorbing material such as titanium oxide and zinc oxide, and an ultraviolet absorbing layer that is a coating film of a paint containing the ultraviolet absorbing material. The thing etc. which comprise are mentioned. When the excitation light radiated from the light source 30 is ultraviolet light, the light shielding sheet 40 may be anything that can shield (shield) at least ultraviolet light, but may further adopt light that can also shield light in the visible light range.

  The bent portion 43 is shaped by bending the sheet-like member constituting the light-shielding sheet 40 to give a bending wrinkle or the like, or forming the sheet-like member into a locally bent shape when the sheet-like member is formed. Is formed.

  FIG. 5 illustrates a configuration in which the movable end 42 of the light shielding sheet 40 is attached and fixed so as to cover only a part of the rod-shaped portion 51 of the movable member 50 in the outer peripheral direction. Examples of means for attaching the light shielding sheet 40 to the movable member 50 include adhesion by an adhesive and mechanical fixation such as screwing.

  Further, the mounting structure of the movable end 42 of the light shielding sheet 40 to the movable member 50 is not limited to this. For example, the movable end 42 of the light shielding sheet 40 is covered with the rod-shaped portion 51 of the movable member 50 so as to cover the outer peripheral surface thereof. A structure in which the rod-shaped portion 51 of the movable member 50 is housed inside a tubular portion in which the movable end 42 of the light shielding sheet 40 is formed in a tubular shape, or a structure in which the rod-shaped portion 51 of the movable member 50 is provided. A configuration in which the movable end 42 of the light shielding sheet 40 is clamped and fixed to the clamp component (however, there is no gap between the rod-shaped portion 51 and the light shielding sheet 40) may be employed. Further, the rod-shaped portion 51 itself of the movable member 50 has a half structure in which the light-shielding sheet 40 is clamped and fixed, and the movable end 42 of the light-shielding sheet 40 is sandwiched and fixed to the rod-shaped portion 51, or the rod-shaped portion 51 is penetrated. A configuration in which the movable end 42 of the light-shielding sheet 40 passes through the provided slit and is fixed to the rod-like portion 51 by adhesive fixing with an adhesive or the like can also be adopted.

  The rod-like portion 51 of the movable member 50 has a light shielding property for shielding excitation light. If the light shielding sheet 40 is provided so as to cover the outside of the rod-shaped portion 51 or the light shielding sheet 40 is provided so as to penetrate the rod-shaped portion 51, the light shielding sheet 40 shields the excitation light from the rod-shaped portion 51. It is possible to ensure the sex. Needless to say, if the rod-shaped portion 51 that does not transmit excitation light is employed, the light-shielding property of the entire rod-shaped portion 51 can be secured without using the light-shielding sheet 40. . Also in the case of the configuration using the light shielding sheet 40 for securing the light shielding property of the rod-shaped portion 51, it is preferable to adopt a configuration that does not transmit excitation light as the rod-shaped portion 51.

The movable member 50 is manually moved along the longitudinal direction of the long hole 27 when an operator who performs an operation such as observation of the electrophoresis gel G using the transilluminator 10 operates the operation handle portion 52 with fingers. It can move freely in the direction (lateral direction).
By moving along the longitudinal direction of the long hole 27 of the movable member 50, the bar-shaped portion 51 passes through the sheet drawing space 22 and is parallel to the translucent plate 21 (specifically, the gel installation surface 21a) (see FIG. 4 (a)). The movable end 42 of the light shielding sheet 40 is also moved along this virtual plane S.

  In FIG. 4A, the base end portions 41 of the pair of light shielding sheets 40 </ b> A and 40 </ b> B are provided at positions facing each other through the sheet drawing space 22. In the illustrated example, the base end portions 41 of the light shielding sheets 40A and 40B are provided at positions corresponding to the virtual plane S (the moving plane of the movable member 50) with respect to the end wall of the housing 20. The position of the end 41 may be a position slightly deviated from the virtual plane S in the vicinity of the virtual plane S.

  The light shielding sheet 40 is moved along the longitudinal direction of the long hole 27 of the movable member 50 (moving in the lateral direction), and the movable member 50 and the end wall of the housing 20 to which the proximal end portion 41 of the light shielding sheet 40 is attached. Expansion and contraction (the longitudinal dimension of the belt-like sheet-shaped member constituting the light shielding sheet 40 is constant. Here, the deformation of the bent portion 43 such as the mountain fold portion 43a and the valley fold portion 43b is caused by the deformation. The distance between the base end portion 41 and the movable end 42 is changed, meaning that the base end portion 41 and the movable end 42 can be freely extended and folded).

  As shown in FIGS. 4B and 4C, the light shielding unit 60 arranges the movable end 43 in the sheet retracting space 22 by moving the movable member 50, so that the light shielding sheet 40 is placed in the sheet retracting space. 22 can be withdrawn. In addition, each light shielding unit 60 can open and close between the light source 30 and the translucent plate 21 independently by moving the movable member 50.

As shown in FIG. 3 and the like, as described above, the operation side end portion 51a that is one end in the longitudinal direction of the rod-shaped portion 51 of the movable member 50 is inserted into the elongated hole 27 of the operation side wall portion 241. It is movable along the longitudinal direction of the long hole 27. The long hole 27 itself functions as a guide portion that guides the movement of the operation side end portion 51 a of the rod-like portion 51.
Further, the guide groove 28 of the back wall portion 244 of the housing 20 is formed so that the back side end portion 51 b of the rod-like portion 51 of the movable member 50 extends in the longitudinal direction (extending direction) of the long hole 27 of the operation side wall portion 241. ) Function as a guide part (hereinafter also referred to as a back end guide part) for guiding the movement along. The rear end guide portion extends (extends in the lateral direction) along the longitudinal direction of the long hole 27.

  The casing 20 has a slot 27 in the movable member 50 (specifically, the rod-shaped portion 51) in the side wall portions 24 on both sides via the sheet retracting space 22, that is, the operation side wall portion 241 and the back wall portion 244 here. It is the structure which comprises the guide parts 27 and 28 for guiding the movement along the longitudinal direction. The movable member 50 is operated by the operator by operating the operation handle portion 52, so that the guide portion (long hole 27) of the operation side wall portion 241 and the guide portion (back end guide portion of the back wall portion 244, in the illustrated example). While being guided by the guide groove 28, it can move along the elongated hole 27 and the back end guide portion (in the illustrated example, the extending direction of the guide groove 28 in the longitudinal direction).

  The rear end guide portion provided on the rear side wall portion 244 of the housing 20 may have any configuration that can guide the movement along the longitudinal direction of the long hole 27 of the rear side end portion 51b of the movable member 50. FIG. It is not limited to the guide groove 28 illustrated in FIG. For example, a long hole is formed at a position corresponding to the long hole 27 of the operation side wall part 241 of the back side wall part 244, and the long hole itself functions as a back end guide part, or attached to the back side wall part 244 in parallel to each other. In addition, a configuration in which a guide groove for guiding the rod-like portion 51 is secured between the two guide rails, a configuration in which a guide rail with a guide groove is attached to the back side wall portion 244, and the like can be adopted.

  Further, as a movable member, a guide having a flange portion projecting from the back end portion 51b of the rod-like portion 51 or a configuration in which a ring that is rotatable around the axis is provided at the back end portion 51b is used as a guide. It is also possible to reduce the movement resistance of the back end 51b of the rod-like portion 51 in the extending direction of the back end guide such as a groove or a guide rail.

On the outer periphery of the operation side end portion 51a of the rod-shaped portion 51 of the movable member 50, the inner surface (housing of the operation side wall portion 241 of the housing 20 is restricted in order to restrict the movable member 50 from coming out of the housing 20. A retaining ring 53 that is in contact with the inner space 26 of the 20) projects in a flange shape. When the movable member 50 moves along the longitudinal direction of the elongated hole 27, the retaining ring 53 slides on the inner surface of the operation side wall portion 241. The retaining ring 53 also functions as a posture maintaining member that keeps the orientation of the rod-like portion 51 of the movable member 50 relative to the operation side wall 241 perpendicular to the operation side wall 241. Therefore, in the transilluminator 10, the movable member 50 can be freely moved along the longitudinal direction of the long hole 27 while the rod-like portion 51 is kept in the vertical direction with respect to the operation side wall portion 241.
Here, the retaining ring 53 is a member fixed in a flange shape around the outer periphery of the operation side end 51 a of the rod-shaped portion 51, but for example, the operation-side end 51 a of the rod-shaped portion 51 It may be a ring-shaped member provided on the outer periphery so as to be rotatable about its axis.

  As illustrated in FIG. 4A, the entire light shielding sheet 40 of the light shielding unit 60 is a space secured between the sheet drawing space 22 and the end wall in the housing 20. In the state of being housed in 29, all the bent portions 43 of the light shielding sheet 40 are bent as shown by the solid line in FIG. Further, in the transilluminator 10, when the entire light shielding sheet 40 is stored in the light shielding sheet storage portion 29, the rod-shaped portion 51 of the movable member 50 to which the light shielding sheet 40 is attached is shielded from the sheet drawing space 22. The sheet 40 can be positioned at a position shifted to the base end 41 side (the rod-like portion 51 enters the light shielding sheet storage portion 29), and the light shielding unit 60 is prevented from entering the sheet drawing space 22. Is possible.

The light shielding sheet storage unit 29 of the transilluminator 10 will be specifically described.
As shown in FIG. 4A, the gel installation plate portion 25 of the housing 20 of the transilluminator 10 has a pair of side wall portions 24 (a pair of end portions) in which the frame plates 25a are located on both sides of the housing 20 in the lateral direction. A projecting portion 25c that is a portion projecting from the portion walls 242 and 243) toward the opposing side wall portions 242 and 243 facing each other. The light shielding sheet storage unit 29 is a space secured between the side wall parts 24 (end walls 242 and 243) located on both lateral sides of the housing 20 and the sheet drawing space 22. It is located between the protruding portion 25c and the bottom plate 23. The base end portion 41 of the light shielding sheet 40 is located in the light shielding sheet storage unit 29.

  As shown in FIG. 1, the longitudinal dimension of the long hole 27 and the back end guide part (in the illustrated transilluminator 10, the guide groove 28 (see FIG. 3)) is larger than the lateral dimension of the sheet pull-in space 22. . In the lateral direction of the transilluminator 10, the elongated hole 27 has a portion corresponding to the extending range in the lateral direction of the sheet pull-in space 22, and a portion extending from this portion to both sides in the lateral direction. The back wall portion 244 is provided with a back end guide portion having a dimension in the extending direction (lateral direction) corresponding to the long hole 27 through the inner space 26 of the housing 20. In addition, the movable ranges (movable ranges in the lateral direction) of the movable members 50A and 50B guided by the long holes 27 and the back end guide portions respectively overlap the entire lateral extending range of the sheet pull-in space 22, Furthermore, it extends from the sheet drawing space 22 so as to enter the light shielding sheet storage portion 29 for storing the light shielding sheet 40 of the light shielding unit 60 provided with the movable member.

The entire light-shielding sheet 40 is stored in the light-shielding sheet storage portion 29, and the rod-shaped portion 51 of the movable member 50 is located at a position shifted from the sheet drawing space 22 toward the base end portion 41 of the light-shielding sheet 40. Hereinafter, it is also referred to as a storage state. In this stored state, since there is no portion of the light shielding unit 60 that has entered the sheet drawing space 22, the light shielding unit 60 does not shield the excitation light irradiated from the light source 30 onto the light transmitting plate 21 and the gel G.
As shown in FIG. 4A, when both of the pair of light shielding units 60A and 60B of the transilluminator 10 are in the retracted state (fully opened state), the light transmitting plate is passed through the sheet drawing space 22 from the light source 30. 21 is irradiated with excitation light 32.

  When the movable member 50 of the light-shielding unit 60 in the retracted state is moved and the light-shielding sheet 40 enters (draws into) the sheet drawing space 22, the light-shielding sheet 40 is bent from the side near the movable end 42. 43 bends gently (see phantom lines in FIG. 5). That is, the opening angle θ of the bent portion 43 is increased. Thereby, the distance from the base end part 41 of the light shielding sheet 40 to the movable end 42 is increased, and the light shielding sheet 40 is in an extended state as compared with the retracted state.

  Conversely, when the light shielding sheet 40 is retracted from the retracted state 22 into the retracted state, one or more bent portions 43 of the light shielding sheet 40 are suddenly bent (the opening angle θ of the bent portion 43 is small). The distance from the base end 41 of the light shielding sheet 40 to the movable end 42 is reduced.

As the transilluminator 10, in order to ensure that the movable member 50 is stopped at a desired position by manual operation of the operation handle 52, for example, as shown in FIG. A flexible contact member 271 that is brought into contact with the operation side end 51a of the rod-like portion 51 of the movable member 50 is provided on the inner surface over the entire length in the longitudinal direction, and the movement resistance of the movable member 50 that moves along the longitudinal direction of the elongated hole 27 is provided. May be increased. The flexible contact member 271 is preferably made of, for example, rubber (natural rubber or synthetic rubber), but is not limited thereto, and is made of synthetic resin other than synthetic rubber or natural fiber such as cotton. The formed one can also be used.
Further, the configuration for increasing the movement resistance of the movable member 50 (movement resistance increasing means) is not limited to the installation of the contact member 271 described above. For example, the inner surface of the long hole 27 is roughened and the rod-shaped portion 51 is formed. Roughening of the outer peripheral surface of the operation side end portion 51a, formation of a rubber coating layer on the outer peripheral surface of the operation side end portion 51a of the rod-like portion 51, and the like can also be employed.

  In order to observe the electrophoretic gel G using the transilluminator 10 and to cut out the portion where the band is located from the gel G, the produced sheet-like electrophoretic gel G is used as the translucent plate 21 as shown in FIG. The gel G is observed from the side opposite to the translucent plate 21 by irradiating the gel G with the excitation light output from the light source 30. And the site | part in which a band is located is cut out from this gel G.

  However, in the case of this transilluminator 10, for example, as shown in FIGS. 4B and 4C, the irradiation range of the excitation light 32 to the gel G by the operation of the operation handle 52 of the movable member 50 of the light shielding unit 60. Is narrowed down to a part of the sheet-like gel G, and the gel G can be observed and cut out for the irradiation range. Then, by repeating the movement of the irradiation range of the excitation light 32 in the gel G, the observation of the band, and the cutting of the gel G a plurality of times, the observation of the band and the cutting of the gel G can be sequentially advanced.

In the case of the illustrated transilluminator 10, the irradiation range of the excitation light 32 from the light source 30 to the translucent plate 21, the gel G, depending on the distance between the movable members 50 (specifically, rod-shaped portions 51) of the pair of light shielding units 60. The irradiation range of the excitation light 32 is determined. The excitation light 32 output from the light source 30 is generated when a pair of movable members 50 (specifically, rod-shaped portions 51) provided in the casing 20 so as to be movable along the longitudinal direction of the long holes 27 are separated from each other. The gel G passes through the space between the pair of movable members 50 (specifically, the bar-shaped portion 51) (hereinafter also referred to as a light irradiation space; reference numeral 22a) and passes through the light transmitting plate 21 and is irradiated onto the gel G.
On the other hand, since the rod-shaped part 51 and the light shielding sheet 40 of the movable member 50 shield the excitation light 32, the part of the gel G that faces the rod-shaped part 51 and the light shielding sheet 40 of the movable member 50 via the light transmitting plate 21 ( Hereinafter, the excitation light 32 is not irradiated to the non-irradiation region. Therefore, in this non-irradiated region, the inconvenience that the nucleic acid in the gel G is damaged by the excitation light 32 can be prevented.

FIG. 9B shows an example of a sheet-like gel G to be irradiated with excitation light by the transilluminator 10. The gel G in the illustrated example is formed in a rectangular sheet shape.
Here, an example of the preparation method of the gel G is demonstrated with reference to Fig.8 (a), (b), and Fig.9 (a).
FIGS. 8A and 8B show an example of a gel preparation jig 70.
The gel preparation jig 70 has a structure in which a U-shaped spacer 72 is sandwiched between a pair of plates 71 and a gap 73 is secured between the pair of plates 71 by the spacer 72. Reference numeral 75 in the drawing denotes a clamp component for clamping the pair of plates 71 and the spacer 72 and maintaining the state in which the spacer 72 is sandwiched between the pair of plates 71. The clamp component 75 is detachable. By removing the clamp component 75, the gel preparation jig 70 can be easily disassembled.

A liquid gel material (for example, polyacrylimide solution) is poured into a region located inside the U-shaped spacer 72 in the gap 73, and a polymerization initiator (for example, tetramethylethylenediamine) and a polymerization accelerator for gelation. By adding (for example, ammonium persulfate) and making it gel, a sheet-like gel G1 (hereinafter also referred to as a gel sheet for electrophoresis) is obtained.
A plurality of sample installation recesses G2 (wells) are formed in a row on the end face of the gel gel G1 for electrophoresis using a recess forming tool such as a comb-like comb 74, and samples are placed in the recesses G2 for sample installation. After installing 80 (see FIG. 9A), electrophoresis is performed by applying a voltage to the gel sheet G1 for electrophoresis. Thereby, the electrophoresis gel G shown in FIG.9 (b) is obtained.
Further, in order to bind the staining agent to the nucleic acid molecules in the gel, for example, the staining solution is added to the electrophoresis gel sheet G1 when the electrophoresis gel sheet G1 is prepared, the use of the sample to which the staining solution is added, the electrical of the sample A method of immersing the gel G after electrophoresis in a staining solution is selectively employed.

The electrophoresis gel G according to the present invention is an electrophoretic gel that has been subjected to electrophoresis for separation of nucleic acids in a sample 80, and is combined with a staining agent that emits fluorescence using ultraviolet light or visible light as excitation light. It refers to those containing nucleic acids.
When a dye that emits fluorescence using ultraviolet light as excitation light (for example, ethidium bromide) is employed as the staining agent, the transilluminator 10 includes a light source 30 that outputs ultraviolet light that excites the staining agent to generate fluorescence as excitation light. Use what you want.

When the gel G obtained by electrophoresis is irradiated with excitation light having a wavelength corresponding to the excitation wavelength of the staining agent, the staining agent bound to the nucleic acid is excited to emit fluorescence and separated from the sample 80 by electrophoresis. The band 81 (see FIG. 9B) of the nucleic acid molecule becomes visible.
As shown in FIG. 9 (b), the gel G that has been electrophoresed is a region in which the substance moved from the sample 80 in the sample installation recess G2 is distributed by electrophoresis (indicated by the phantom line in FIG. 9 (b)). The shown lane 82) exists in a band shape extending from the sample installation recess G2. In the gel G, there are parallel lanes 82 for the sample installation recesses G2. A band 81 exists in the lane 82.

When the electrophoretic gel G is observed and cut out using the transilluminator 10, the gel G taken out from the gel preparation jig 70 is a rod-shaped portion of the movable member 50 whose longitudinal direction (extending direction) of the plurality of lanes 81 is In the direction aligned with the longitudinal direction of 51, it is installed so as to overlap the gel installation surface 21a of the translucent plate 21.
Accordingly, for example, as shown in FIGS. 4B and 4C, the irradiation range of the excitation light 32 to the gel G is reduced by operating the operation handle 52 of the movable member 50 of the light shielding unit 60. In the case of narrowing down to a part, it is possible to irradiate the excitation light 32 only to a range corresponding to one selected from a plurality of lanes 82 (see FIG. 9B). Further, the lane 82 for irradiating the excitation light 32 can be changed by operating the operation handle portion 52 of the movable member 50 of the light shielding unit 60. Irradiation of the excitation light 32 to the gel G, observation and cutting of the gel G can be performed in units of lanes 82.

The narrowing of the irradiation range of the excitation light 32 onto the gel G is realized by pulling out one or both of the pair of light shielding units 60A and 60B of the transilluminator 10 from the light shielding sheet storage portion 29 and drawing them into the sheet drawing space 22. The
As described above, the gel G is superposed on the gel installation surface 21 a of the translucent plate 21 such that the longitudinal direction (extending direction) of the plurality of lanes 81 is aligned with the longitudinal direction of the rod-like portion 51 of the movable member 50. If the size of the light irradiation space 22a secured between the pair of light shielding units 60 is determined by adjusting the position of the movable member 50 of the light shielding unit 60A and the movable member 50 of the light shielding unit 60B, excitation is performed. The lane 82 for irradiating the light 32 can be selected.

FIG. 4B shows the light shielding unit 60A in the retracted state, the light shielding sheet 40 of the light shielding unit 60B on the opposite side is drawn into the sheet drawing space 22, and the light irradiation space 22a is interposed between the pair of light shielding units 60A and 60B. 4 (c) shows the light shielding unit 60B in the retracted state, the light shielding sheet 40 of the light shielding unit 60A on the opposite side is drawn into the sheet drawing space 22, and the space between the pair of light shielding units 60A and 60B. The state where the light irradiation space 22a is secured is shown.
1 and 2 show a state in which the light shielding sheets 40 of the two light shielding units 60A and 60B are respectively drawn into the sheet drawing space 22, and the light irradiation space 22a is secured between the pair of light shielding units 60A and 60B. Show.

  Although not shown, the pair of light shielding units 60A and 60B can be closed by bringing the movable members 50 into contact with each other. At this time, since the light irradiation space 22a is not secured, the gel G is not irradiated with the excitation light. The pair of light shielding units 60 </ b> A and 60 </ b> B can open and close between the light source 30 and the translucent plate 21.

As described above, when the irradiation of the excitation light to the gel G, the observation and cutting of the gel G are performed in units of lanes 82, in the non-irradiation region where the excitation light in the gel G is not irradiated, the gel G is irradiated by the excitation light. It is possible to prevent inconvenience such as damage of the nucleic acid therein.
As a result, it becomes possible to secure a sufficient work time for cutting out the part where the gel band is located, so that it is possible to reduce the case of failing to cut out due to work, and to improve the work certainty of cutting. .

  In addition, irradiation of the excitation light to the gel G, observation and cutting of the gel G are not limited to being performed in units of lanes 82 (every lane), for example, for two or more lanes 82 adjacent to each other. There is no particular limitation.

(Second Embodiment)
Next, a transilluminator 10A according to a second embodiment of the present invention will be described with reference to FIG. 10, FIG. 11 (a), and (b). In addition, the same code | symbol is attached | subjected and demonstrated to the same component as 1st Embodiment.
As shown in FIG. 10, this transilluminator 10 </ b> A has an appearance box-shaped housing 20 </ b> A, similar to the transilluminator 10 of the first embodiment. As shown in FIGS. 10, 11A, and 11B, the housing 20A is similar to the housing 20 of the transilluminator 10 of the first embodiment. It has a side wall 24 '. Further, the four side wall portions 24 ′ correspond to the four side wall portions 24 of the housing 20 of the transilluminator 10 of the first embodiment, and the operation side wall portions 241 ′, the end walls 242 ′, 243 ′, the back It may be described as a wall portion 244 ′. This housing 20A has dimensions in the direction perpendicular to the bottom plate 23 and the gel installation plate 25 of the four side wall portions 24 ′ compared to the four side wall portions 24 of the housing 20 of the transilluminator 10 of the first embodiment. The distance between the bottom plate 23 and the gel installation plate 25 is larger than that of the casing 20 of the transilluminator 10 of the first embodiment, not only the operation side wall portion 241 ′ but also a pair of end walls. One of 242 ′ and 243 ′ (here, an end wall denoted by reference numeral 242 ′. Hereinafter, the end wall 242 ′ is also referred to as a first end wall, and the opposite end wall 243 ′ is also referred to as a second end wall. ) Is also different from the case 20 of the transilluminator 10 of the first embodiment in that a long hole (second long hole 27A) is formed.
Further, the transilluminator 10A of this embodiment differs from the transilluminator 10 of the first embodiment in that the housing 20A is used and two light shielding units 90 are added.

Hereinafter, the light shielding unit 90 is also referred to as an auxiliary light shielding unit. For convenience of explanation, one of the two auxiliary light shielding units 90 may be described with reference numeral 90A and the other with reference numeral 90B.
Hereinafter, the light shielding unit 60 including the movable member 50 that is movable along the longitudinal direction (extending direction) of the long hole 27 of the operation side wall portion 241 ′ is also referred to as a main light shielding unit.

In the transilluminator 10A, the direction along the longitudinal direction of the elongated hole 27 is referred to as a lateral direction, and the direction perpendicular to the lateral direction and along the light transmitting plate 21 (specifically, the gel installation surface 21a) is referred to as a longitudinal direction. It is the same as in the first embodiment.
The second long hole 27A is formed to extend in the vertical direction on the first end wall 242 ′ of the housing 20A.

  As shown in FIGS. 10 and 11B, in the transilluminator 10A, the two auxiliary light shielding units 90 each have a belt-like flexible sheet-like member that can shield the excitation light output from the light source 30. A light-shielding sheet 91 (auxiliary light-shielding sheet) formed with a mountain fold and a valley fold, and a base end attached to the casing 20A at one end in the longitudinal direction of the sheet-like member constituting the light-shielding sheet 91 A movable member 92 (auxiliary movable member) attached to the end opposite to 91a is provided.

  As the light shielding sheet 91 of the auxiliary light shielding unit 90, one having the same structure as the light shielding sheet 40 of the main light shielding unit 60 can be used. The mountain folds and the valley folds are formed in a line parallel to each other in a direction perpendicular to the longitudinal direction of the sheet-like member, and a plurality of folds are formed so as to be alternately arranged in the longitudinal direction of the sheet-like member. Yes. 10 and FIG. 11B, the mountain fold portion refers to a bent portion protruding upward, and the valley fold portion refers to a bent portion protruding downward in FIG. 10 and FIG. 11B. The light shielding sheet 91 is housed in the housing 20A in such a direction that the mountain folds and the valley folds extend in the horizontal direction.

The light shielding sheet 91 of the auxiliary light shielding unit denoted by reference numeral 90A (indicated by reference numeral 91A in FIGS. 10 and 11B) has one longitudinal end (base end portion 91a) of the sheet-like member constituting the light shielding sheet 91A. The movable member 92 (movable member denoted by reference numeral 92A in the figure) is attached to the operation side wall portion 241 'of the housing 20A, and the end portion (movable end 91b) of the sheet-like member opposite to the base end portion 91a. It becomes the structure which attached.
The light shielding sheet 91 of the auxiliary light shielding unit denoted by reference numeral 90B (indicated by reference numeral 91B in FIGS. 10 and 11B) has one end in the longitudinal direction of the sheet-like member constituting the light shielding sheet 91B as the operation side wall of the housing 20. The movable member 92 (movable member denoted by reference numeral 92B in the figure) is attached to the end portion (movable end 91b) of the sheet-like member opposite to the base end portion 91a. ing.

  As shown in FIG. 11B, a base end portion 91a fixed to the housing 20A of one light shielding sheet 91A of the pair of auxiliary light shielding units 90A and 90B, and a base fixed to the housing 20 of the other light shielding sheet 91B. The end portion 91a is located at a position facing the end portion 91a through the sheet drawing space 22 in a direction along the longitudinal direction of the second long hole 27A. The base end portions 91a of the light shielding sheets 91 of the two auxiliary light shielding units 90 are both provided at positions avoiding the sheet drawing space 22 in the housing 20 in the direction along the longitudinal direction of the second long hole 27A. Yes.

  As shown in FIGS. 10 and 11A, the movable member 92 of the auxiliary light shielding units 90A and 90B is parallel to the operation side wall 241 ′ (perpendicular to the pair of end walls 242 ′ and 243 ′ of the housing 20A). ) And a bar-like portion 92a provided so as to span the side wall portions 24 ′ (end wall 242 ′, 243 ′) on both sides via the sheet pull-in space 22, and the first portion of the bar-like portion 92a. 2 includes an operation handle portion 92c protruding from the end (hereinafter also referred to as operation side end portion 92b) inserted into the long hole 27A to the outside of the housing 20A.

  The attachment structure of the light shielding sheet 91 to the movable member 92 may be the same as the attachment structure of the light shielding sheet 40 to the movable member 50 in the main light shielding unit 60 (see FIG. 5).

The rod-like portion 92a of the movable member 92 can freely move in the longitudinal direction along the longitudinal direction of the second elongated hole 27A while the operation side end portion 92b is guided by the second elongated hole 27A. The second long hole 27A functions as a guide portion (second operation side guide portion) for guiding the operation side end portion 92b of the rod-like portion 92a of the movable member 92.
An end of the movable member 92 opposite to the operation side end 92b (hereinafter also referred to as a back side end 92d) of the rod-like portion 92a is a first end on the second end wall 243 ′ of the housing 20A. The second back end guide while being guided by a guide portion (hereinafter also referred to as a second back end guide portion; in FIG. 11A, a guide groove 28A) formed to extend in parallel with the second long hole 27A of the wall 242 ′. It moves freely in the extending direction of the part (longitudinal direction of the second long hole 27A).

  In other words, the transilluminator 10A has side wall portions 24 ′ on both sides via the sheet drawing space 22 of the housing 20A in the direction (longitudinal direction) along the longitudinal direction (extending direction) of the second long hole 27A, that is, Here, a guide portion for guiding the movement along the longitudinal direction of the second long hole 27A of the movable member 92 (specifically, the rod-shaped portion 92a) to the first end wall 242 ′ and the second end wall 243 ′. It has composition which comprises. The movable member 92 is configured such that the operator operates the operation handle portion 92c, whereby the guide portion (second long hole 27A) of the first end wall 242 ′ and the guide portion (first portion of the second end wall 243 ′). The second operation side guide part (specifically, the second long hole 27A) and the second back end guide part are moved along the extending direction (moved in the vertical direction) while being guided by the second back end guide part). it can.

  FIG. 11A illustrates a guide groove 28A formed as a second back end guide portion in the second end wall 243 ′ so as to extend in parallel to the second long hole 27A of the first end wall 242 ′. ing. The rear end portion 92d of the rod-shaped portion 92a is inserted into the guide groove 28A, and can freely move along the longitudinal direction (extending direction) of the guide groove 28B while being guided by the guide groove 28A.

  However, the second back end guide part is not limited to the guide groove 28A, and may be any structure that can guide the movement along the longitudinal direction of the second long hole 27A of the back end part 92d of the movable member 92, It is not limited to the guide groove 28A illustrated in FIG. As the second back end guide portion, a configuration using a guide rail similar to the back end guide portion for guiding the back side end portion of the movable member 50 of the main light shielding unit 60, or a sheet on the second end wall 243 ′ is used. An elongated hole formed at a position corresponding to the second elongated hole 27 through the drawing space 22 can also be adopted. Further, as the movable member 92, one having a configuration in which a flange portion projects from the rear end portion 92d of the rod-shaped portion 92a or a configuration having a ring that is rotatable around the axis of the rear end portion 92d is adopted. In addition, it is possible to reduce the movement resistance of the back end 92d of the rod-shaped portion 92a in the extending direction of the second back end guide portion such as a guide groove or a guide rail.

An inner surface of the first end wall 242 ′ of the housing 20A is provided on the outer periphery of the operation side end portion 92b of the rod-shaped portion 92a of the movable member 92 in order to restrict the movable member 92 from coming out of the housing 20 outside. A retaining ring 92e that is in contact with the (surface facing the inner space 26 ′ of the housing 20A) protrudes. When the movable member 92 moves along the longitudinal direction of the second elongated hole 27A, the retaining ring 92e slides on the inner surface of the first end wall 242 ′. The retaining ring 92e also functions as a posture maintaining member that maintains the orientation of the rod-shaped portion 92a of the movable member 92 relative to the first end wall 242 ′ perpendicular to the first end wall 242 ′.
Therefore, in this transilluminator 10A, the movable member 92 freely moves along the longitudinal direction of the second elongated hole 27A while the rod-shaped portion 92a is kept perpendicular to the first end wall 242 ′. Can be made.
In addition, the retaining ring 92e of the movable member 92 in the illustrated example is a member fixed to the entire outer periphery of the rod-shaped portion 92a. For example, it can freely rotate around the outer periphery of the operation-side end portion 92b of the rod-shaped portion 92a. It may be a ring-shaped member provided in the.

The movable member 92 is manually moved in the longitudinal direction of the second elongated hole 27A by an operator who performs an operation such as observation of the electrophoretic gel G using the transilluminator 10A and manually operates the operation handle portion 92c. Can move freely along the direction.
Movement along the longitudinal direction of the second elongated hole 27A of the movable member 92 is movement within a virtual plane S1 that passes through the sheet drawing space 22 and is parallel to the light transmitting plate 21 (specifically, the gel installation surface 21a). is there. With the movement of the movable member 92, the movable end 91b of the light shielding sheet 91 is moved along the virtual plane S1.

In the illustrated transilluminator 10A, a configuration in which the pair of auxiliary light shielding units 90 are provided at positions shifted from the pair of main light shielding units 60 toward the bottom plate 23 of the housing 20A is illustrated.
However, in the transilluminator according to the present embodiment, a configuration in which the pair of auxiliary light shielding units 90 are provided at positions shifted from the pair of main light shielding units 60 toward the gel installation plate 25 side of the housing 20A can be employed.

The movable member 92A of the auxiliary light shielding unit denoted by reference numeral 90A and the movable member 92B of the auxiliary light shielding unit denoted by reference numeral 90B are provided on a pair of guide portions (second operation side guide portion and second rear end guide portion) provided on the housing 20A. By being guided, each can move independently along the longitudinal direction of the second elongated hole 27A.
In the light shielding sheet 91 of the auxiliary light shielding units 90A and 90B, the movable member 92 to which the movable end 91b of the light shielding sheet 91 is attached is moved in the vertical direction, and the base end portion 91a of the light shielding sheet 91 and the movable member 92 are moved. Expansion and contraction (longitudinal dimensions of the belt-like sheet-shaped member constituting the light shielding sheet 91 are constant. Here, the base end portion 91a and the base end portion 91a are deformed by bending portions such as a mountain fold portion and a valley fold portion. It means that the distance to the movable end 91b fluctuates, meaning that it can be extended and folded freely).
The auxiliary light shielding units 90 </ b> A and 90 </ b> B can open and close between the light source 30 and the translucent plate 21 independently by moving the movable member 92.

  As the transilluminator 10A, in order to ensure that the movable member 90 is stationary at a desired position by manual operation of the operation handle portion 92c, for example, the second long illuminator is similar to the long hole 27 of the transilluminator 10 of the first embodiment. A flexible contact member is provided on the inner surface of the hole 27A in the longitudinal direction along the longitudinal direction of the second elongated hole 27A by providing a flexible contact member to be brought into contact with the operation side end 92b of the rod-shaped portion 92a of the movable member 92. The movement resistance of the movable member 90 to be increased may be increased. The material of the contact member can be the same as that described in the transilluminator 10 of the first embodiment. The configuration for increasing the movement resistance of the movable member 90 (movement resistance increasing means) is not limited to the installation of the contact member described above. For example, the inner surface of the second long hole 27A is roughened, and the rod-shaped portion 92a. It is also possible to employ roughening of the outer peripheral surface of the operation side end portion 92b, formation of a rubber coating layer on the outer peripheral surface of the operation side end portion 92b of the rod-shaped portion 92a, and the like.

The two auxiliary light shielding units 90 provided in the transilluminator 10 </ b> A each independently cover the entire back surface side of the light transmitting plate 21 so that the excitation light output from the light source 30 is not irradiated onto the light transmitting plate 21. Can be shielded from light.
Further, in the housing 20A of the transilluminator 10A, auxiliary light shielding sheet storage portions 29A, which are spaces for accommodating the light shielding sheets 91 of the auxiliary light shielding units 90, are provided on both sides in the vertical direction via the sheet drawing space 22. It is secured.

  As shown in FIG. 11B, the gel installation plate portion 25 of the housing 20 of the transilluminator 10A has a pair of side wall portions 24 ′ (operation side walls) in which the frame plates 25a are positioned on both sides in the longitudinal direction of the housing 20. A projecting portion 25d which is a portion projecting from the portion 241 ′ and the rear wall portion 244 ′) toward the opposing side wall portions 241 ′ and 244 ′ facing each other. The auxiliary light-shielding sheet storage portion 29A is a space secured between the side wall portions 24 ′ located on both sides in the longitudinal direction of the housing 20A and the sheet drawing space 22, and the overhanging portion 25d and the bottom plate of the housing 20A. 23. The base end portion 91a of the light shielding sheet 91 is located in the auxiliary light shielding sheet storage portion 29A.

  As shown in FIG. 10, the longitudinal dimension of the second long hole 27 </ b> A and the second back end guide portion is larger than the longitudinal dimension of the sheet drawing space 22. In the longitudinal direction of the transilluminator 10A, the second long hole 27A and the second back end guide portion are extended from the portion corresponding to the longitudinal extension range of the sheet pull-in space 22 and both sides in the longitudinal direction. Part. The movable ranges (movable ranges in the vertical direction) of the movable members 92A and 92B guided by the second elongated hole 27A and the second back end guide portion respectively overlap the entire vertical extending range of the sheet drawing space 22. At the same time, it extends from the sheet drawing space 22 into the auxiliary light shielding sheet storage portion 29A for accommodating the light shielding sheet 91 of the auxiliary light shielding unit 90 provided with the movable member. For this reason, the auxiliary light-shielding units 90A and 90B bring the movable member 92 close to the base end portion 91a of the light-shielding sheet 91 in which the movable end 91b is attached to the movable member 92. 29A, and can be stored in the sheet pull-in space 22 and shifted to the base end portion 91a side of the light-shielding sheet 91, and there is no portion that has entered the sheet pull-in space 22 (FIG. 11B). State, fully open state, and stored state).

Since the transilluminator 10A includes two auxiliary light shielding units 90A and 90B in addition to the two main light shielding units 60A and 60B, the light source 30 in the gel G installed on the gel installation surface 21a of the translucent plate 21. Can be narrowed down to a desired range in the lateral direction (hereinafter also referred to as a horizontal diaphragm) by the light irradiation space 22a between the two main light-shielding units 60A and 60B. The light irradiation space 22b between the auxiliary light shielding units 90A and 90B can be narrowed down to a desired range in the vertical direction (hereinafter also referred to as a vertical diaphragm). Therefore, the irradiation range of the excitation light in the gel G can be narrowed down to a narrower range than the transilluminator 10 of the first embodiment.
As a result, nucleic acids that are damaged by irradiation with excitation light can be reduced.

For example, when performing operations such as observing and cutting out the gel G, the irradiation range in which the gel G is irradiated with excitation light by adjusting the position of the movable member 50 (lateral position adjustment) of the two main light shielding units 60A and 60B. The target lane 82 is narrowed down (horizontal stop), and the irradiation range where the gel G is irradiated with the excitation light is cut out by adjusting the position of the movable member 92 (vertical position adjustment) of the two auxiliary light shielding units 90A and 90B. It is possible to narrow down to the target band to be performed and the vicinity thereof (vertical diaphragm).
Moreover, there is also an advantage that the comparative observation of the portions having the same distance from the sample installation recess G2 can be easily performed with respect to the plurality of lanes 82 (see FIG. 9B) of the gel G by the vertical diaphragm of the irradiation range.

(Third embodiment)
Next, a transilluminator according to a third embodiment of the present invention will be described with reference to FIG.
As shown in FIG. 12, this transilluminator 10B is obtained by omitting one of the pair of light shielding units 60A and 60B of the transilluminator 10 of the first embodiment (in FIG. 1, the light shielding unit 60A is omitted). .
In the figure, the same components as those in FIG.

  In the case of the transilluminator 10B, for example, the gel G is placed so as to be superimposed on the light transmitting plate 21 (the extending direction of the lane 82 of the gel G is aligned with the longitudinal direction of the transilluminator). After the gel G is observed and cut out so that only the leftmost lane 82 is irradiated with the excitation light output from the light source 30, the movable member 50 of the light unit 60B is accommodated with the light shielding sheet 40 of the light shielding unit 60B. To the light shielding sheet storage unit 29 side (right side in FIG. 12), the irradiation area of the excitation light in the gel G is extended to the lane 82 adjacent to the right of the lane 82 at the left end of the gel G in FIG. The gel G is observed and cut out, and the movement of the movable member 50 and the observation and cut out of the gel G are sequentially performed in units of lanes 82. Possible it is.

  However, when considering the reexamination for re-observation and analysis of the gel G cut-out lane, the nucleic acid in the cut-out lane should not be affected by the irradiation of excitation light. In addition, it is preferable to block the irradiation of the excitation light to the cut-out lane. In this respect, it is preferable to employ the transilluminator of the first or second embodiment rather than the transilluminator of the third embodiment.

(Fourth embodiment)
Next, a fourth embodiment according to the present invention will be described with reference to FIGS. 13 (a) and 13 (b).
In the above-described first to third embodiments, as the light shielding sheet, for example, as shown in FIGS. 5 and 6, bent portions (mountain folds, valley folds) are formed at a plurality of positions in the longitudinal direction of the belt-like sheet-like member. Although the thing was illustrated, as a light shielding sheet which concerns on this invention, it is not limited to this, The thing in which the bending part is not formed is also employable. For example, a belt-like flexible sheet-like member in which no bent portion is formed may be used as the light shielding sheet.

  When a belt-like flexible sheet-like member is used as a light-shielding sheet, for example, as shown in FIGS. 13A and 13B, a sheet support member for supporting the light-shielding sheet 110 is provided in the housing 20 to shield the light. It is preferable to support a plurality of locations in the longitudinal direction of the sheet (sheet-like member). The sheet supporting member is provided so as to be movable in the same direction as the moving direction (longitudinal direction of the long hole) of the movable member attached to the light shielding sheet.

Here, first, a transilluminator having a configuration in which two light shielding units 112 having a sheet support bar denoted by reference numeral 111 as a sheet support member are provided in place of the two light shielding units 60 of the transilluminator 10 of the first embodiment. Will be described. In FIG. 13B, reference numeral 10C denotes a transilluminator.
The light shielding unit 112 has a movable member 50 at a movable end 110b opposite to the base end portion 110a attached to the side wall portion 24 (the end wall 242 in FIG. 13A) of the housing 20 of the light shielding sheet 110. The sheet support rods 111 are attached to a plurality of positions spaced apart from each other in the longitudinal direction of the light shielding sheet 110, and a strip-like flexible sheet-like member is used as the light shielding sheet 110. This is different from the light shielding unit 60 described in the first embodiment in that the sheet support bar 111 is attached.
In this transilluminator 10C, the base end portion 110a of the light shielding sheet 110 is replaced with an end wall denoted by reference numeral 242 in place of the first light shielding unit 60A of the transilluminator 10 of the first embodiment (see FIGS. 4A to 4C). The base end portion 110a of the light shielding sheet 110 is replaced with an end wall denoted by reference numeral 243 (FIG. 4A to FIG. 4A) in place of the light shielding unit 112 attached to the second light shielding unit 60B of the transilluminator 10 of the first embodiment. This is a configuration in which a light shielding unit 112 attached to (c) is provided.

  Both ends of the sheet support rod 111 are guided by a pair of guide portions (in the illustrated example, a long hole 27 and a guide groove 28) for guiding the rod-shaped portion 51 of the movable member 50, so that the movable member 50 and Similarly, it is movable in the horizontal direction (left and right direction in FIG. 13A). In the illustrated example, one end in the longitudinal direction of the sheet support bar 111 is inserted into the elongated hole 27 so as to be movable in the longitudinal direction of the elongated hole 27, and the other longitudinal end is inserted into the guide groove 28. 28 is movable in the longitudinal direction.

  Reference numeral 113 in the figure denotes a retaining ring that protrudes in the form of a flange on the outer periphery of the sheet support bar 111, and a side wall portion 24 (in the illustrated example, an operation side wall portion) in which a long hole 27 is formed. 241) is in contact with the inner surface (the surface facing the inner space 26 of the housing 20). When the sheet support bar 111 moves while being guided by the guide portion, the contact member 113 is slid on the side wall portion 24 in which the long hole 27 is formed.

The light shielding unit 112 in the illustrated example can also freely extend and fold the light shielding sheet 110 by moving the movable member 50 along the longitudinal direction of the long hole 27. By moving the movable member 50 closer to the base end portion 110 a side of the light shielding sheet 110, the entire light shielding sheet 110 can be stored in the light shielding sheet storage portion 29, and the movable member 50 is disposed at a position that does not enter the sheet drawing space 22. be able to. At this time, as the distance between the sheet support bars 111 is shortened, the light shielding sheet 110 is bent with a small radius between the sheet support bars 111, and the light shielding sheet 110 is folded (in a folded state). Stored inside. On the contrary, the light shielding sheet 110 stored in the light shielding sheet storage unit 29 can be pulled into the sheet drawing space 22 so as to be extended from the folded state by the moving operation of the movable member 50.
This light shielding unit 112 can also be applied to the auxiliary light shielding unit described in the second embodiment. Needless to say, the present invention can also be applied as a light shielding unit of the transilluminator described in the third embodiment.

In addition, this invention is not limited to embodiment mentioned above, A change is possible suitably.
In the above-described embodiment, in order to guide both ends of the rod-shaped portion of the movable member, a pair of guide portions (a long hole and a back-end guide portion or a long pair) corresponding to both ends of the rod-shaped portion of the movable member on the casing. However, the present invention is not limited to this, and only one of the both ends of the rod-shaped portion of the movable member is used as the guide portion for guiding the movement of the movable member. It is also possible to adopt a configuration provided corresponding to the above.
In the above-described embodiment, the configuration in which the retaining ring provided on the movable member functions as the posture maintaining member is illustrated. However, in the present invention, the movable member is provided with a projecting elongated hole of the operation handle portion of the movable member. Any structure can be used as long as it is movable along the longitudinal direction of the long hole by the guide portion while maintaining a vertical direction with respect to the side wall portion of the formed casing, and the posture of the movable member is maintained. There are no particular limitations on the configuration of the means (posture maintaining means) and the guide section. For example, a configuration in which the guide portion itself functions as the posture maintaining means of the movable member can be employed. In this case, a configuration in which the retaining ring is omitted can also be employed.

Further, in the above-described embodiment, the movable member having the operation handle portion at the position where the operation side end portion of the rod-shaped portion is extended to the outside of the housing is illustrated, but the movable member according to the present invention is not limited thereto. For example, as shown in FIG. 14, a rod-shaped portion 101 provided in the housing 20, and an operation handle portion 102 protruding in an L shape from the outer peripheral surface of the operation-side end portion 101 a of the rod-shaped portion 101. It is also possible to employ the movable member 100 having a configuration including:
In this case, in addition to the guide portion (the long hole 245 in FIG. 14) for guiding the operation side end portion 101 a of the rod-like portion 101 to the side wall portion 24 of the case, the operation handle portion 102 is placed outside the case 20. By adopting a configuration in which a long hole 246 for projecting is formed in parallel with the long hole 245, the movable member 100 is moved in the longitudinal direction of the long hole 246 through which the operation handle portion 102 is inserted by operating the operation handle portion 102. To move along. The elongated hole 246 for the operation handle portion 102 can also function as a guide portion for guiding the movement of the movable member 100.
The guide portion for guiding the operation side end of the rod-like portion of the movable member is not limited to the long hole 245, and a guide rail or a guide groove may be employed.

As the movable member according to the present invention, the portion (sheet attachment portion) to which the movable end of the light shielding sheet is attached is moved along the elongated hole by the operation of the operation handle portion on the outside of the housing. Any structure may be used as long as the movable end can be moved along the long hole in the side wall of the housing, and the structure is not necessarily limited to a structure having a rod-shaped portion (sheet attachment portion).
For example, a block-shaped seat mounting portion that is movably provided along the elongated hole in the casing between the sidewall portion (side wall portion of the casing) in which the elongated hole is formed and the sheet drawing space 22. (Seat mounting block) and an operation handle portion protruding from the elongated hole and projecting to the outside of the housing, and the operating handle portion is moved in the longitudinal direction of the elongated hole. Thus, it is possible to adopt a configuration in which the sheet attaching portion and the movable end of the light shielding sheet attached to the sheet attaching portion are moved integrally with the operation handle portion.

The transilluminator according to the present invention is not limited to the one used in the horizontal orientation, but can be applied to one used in the vertical orientation (direction in which the gel installation surface is up and down).
The transilluminator exemplified in the first to fourth embodiments can be used not only horizontally but also vertically.

It is a front view which shows the transilluminator of 1st Embodiment which concerns on this invention. It is a perspective view which shows the transilluminator of FIG. FIG. 2 is a cross-sectional view taken along line AA in FIG. 1. (A)-(c) of the excitation light in the electrophoretic gel installed in the translucent plate and the gel installation surface of the translucent plate by the change of the position of the movable end of the light shielding sheet provided in the transilluminator of FIG. It is a figure explaining the change of an irradiation range. It is a figure which expands and shows the structure of the movable member vicinity of the light-shielding unit provided in the transilluminator of FIG. It is a top view which shows the structure of the light-shielding unit provided in the transilluminator of FIG. It is a figure which shows an example of the structure which provided the contact member in the long hole inner surface formed in the side wall part of the housing | casing of the transilluminator of FIG. It is a figure which shows an example of the jig | tool for gel preparation, Comprising: (a) is a front view, (b) is a BB sectional view taken on the line of (a). (A) is a figure explaining the electrophoresis of the nucleic acid using the jig for gel preparation of FIG. 8, (b) is a figure which shows the gel after electrophoresis. It is a perspective view which shows the transilluminator of 2nd Embodiment which concerns on this invention. It is sectional drawing which shows typically the internal structure of the transilluminator of FIG. 10, (a) is a front sectional view, (b) is a sectional side view. It is a front view which shows the transilluminator of 3rd Embodiment which concerns on this invention. It is a figure explaining 4th Embodiment which concerns on this invention, Comprising: (a) is a figure which shows a light-shielding unit, (b) is sectional drawing which shows the structure of a transilluminator. It is a figure which shows the other example of the movable member used for the transilluminator which concerns on this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 ... Transilluminator for electrophoretic gels, 20 ... Housing, 21 ... Translucent plate, 21a ... Gel installation surface, 21b ... Back surface, 22 ... Space secured between translucent plate and light source (sheet drawing space) ), 22a ... light irradiation space, 23 ... bottom plate, 24 ... side plate, 241 ... side plate (operation side wall), 242 ... side wall (end wall, first end wall), 243 ... side wall (end) Part wall, second end wall), 244 ... side wall part (back wall part), 25 ... gel installation plate, 25a ... frame plate, 25b ... opening, 25c, 25d ... projecting part, 26 ... inner space, 27 ... Long hole, guide part, 28 ... guide part (guide groove), 29 ... light shielding sheet storage part, 30 ... light source, 31 ... switch, 32 ... excitation light, 40 ... light shielding sheet, 40A ... light shielding sheet, first light shielding sheet, 40B ... light shielding sheet, second light shielding sheet, 41 ... base end portion, 42 ... movable end 43 ... Bending part, 43a ... Mountain folding part, 43b ... Valley folding part, 50 ... Movable member, 50A ... Movable member, first movable member, 50B ... Movable member, second movable member, 51 ... Bar-shaped part, 51a ... Operation Side end, 51b ... back side end, 52 ... operation handle, 53 ... retaining ring, 60 ... light shielding unit, 60A ... light shielding unit, first light shielding unit, 60B ... light shielding unit, second light shielding unit,
10A ... transilluminator for electrophoresis gel, 20A ... housing, 24 '... side plate, 241' ... side plate (operation side wall), 242 '... side wall (end wall, first end wall), 243' ... Side wall part (end wall, second end wall), 244 '... side wall part (back wall part), 26' ... inner space, 27A ... guide part, second elongated hole, 28A ... guide part (guide groove), 29A: Auxiliary light shielding sheet storage unit, 90, 90A, 90B ... Light shielding unit, 91, 91A, 91B ... Light shielding sheet, 91a ... Base end, 91b ... Movable end, 92, 92A, 92B ... Movable member, 92a ... Rod-shaped part 92b: operation side end, 92c ... operation handle, 92d ... back side end, 92e ... retaining ring,
10B ... transilluminator for electrophoresis gel,
DESCRIPTION OF SYMBOLS 100 ... Movable member, 101 ... Bar-shaped part, 101a ... Operation side edge part, 102 ... Operation handle part, 245 ... Guide part (elongate hole), 246 ... Elongate hole,
10C: transilluminator for electrophoresis gel, 110: light shielding sheet, 110a: base end, 110b: movable end, 111: sheet support rod, 112: light shielding unit, 113: retaining ring.

Claims (6)

A transilluminator for electrophoresis gel that irradiates an electrophoresis gel of nucleic acid or protein with excitation light that excites a fluorescent staining agent that stains the nucleic acid or protein molecule in the gel to generate fluorescence,
A housing, a light source that is housed in the housing and outputs the excitation light, and a space secured between the light source and a light transmitting plate that forms part of the housing and transmits the excitation light. Attached to an end of the light-shielding sheet opposite to a base end attached to the casing, and the light-transmitting sheet is perpendicular to the light-transmitting plate in the casing. A movable member that is moved in the longitudinal direction of a long hole that extends along the translucent plate in a side wall provided so as to surround the space between the plate and the light source, The electrophoresis gel is installed on the gel installation surface, which is the surface opposite to the inner space of the casing of the translucent plate,
The movable member has an operation handle portion that protrudes to the outside of the casing through the elongated hole,
The base end portion of the light shielding sheet is provided at a position avoiding the space between the light source and the translucent plate in the longitudinal direction of the elongated hole, and the transparent member is moved from the light source by moving the movable member. A transilluminator for electrophoresis gel, wherein an irradiation range of the excitation light to the light plate is changed. A first light-shielding sheet that is the light-shielding sheet having the base end portion on one of both sides through the space between the light source and the light-transmitting plate in the longitudinal direction of the elongated hole, and the first light-shielding sheet The first movable member, which is the movable member attached to the end opposite to the base end, and the first light shielding sheet via the space between the light source and the translucent plate A second light-shielding sheet that is the light-shielding sheet having the base end on the opposite side, and a second movable member that is attached to the end of the second light-shielding sheet opposite to the base end. A movable member,
The operation handle portion of the first movable member and the operation handle portion of the second movable member are projected to the outside of the casing through the elongated hole. Transilluminator for electrophoresis gel.   Furthermore, in the extending direction of this 2nd long hole, the 2nd long hole formed in the said side wall part of the said housing along the said translucent board in the direction orthogonal to the longitudinal direction of the said long hole, The auxiliary light-shielding sheet, which is the light-shielding sheet attached to the housing at a position avoiding the space between the light source and the light-transmitting plate, and the auxiliary light-shielding sheet attached to the housing The auxiliary movable member which is the movable member which is attached to the edge part on the opposite side to a base edge part, and is moved along the extension direction of the said 2nd long hole is characterized by the above-mentioned. 2. A transilluminator for electrophoresis gel according to 2. The movable member includes a rod-shaped portion provided on the side wall portion located on both sides through a space secured between the light-transmitting plate and the light source in the housing, and the rod-shaped portion. The operation handle portion protruding,
The transilluminator for electrophoretic gel according to any one of claims 1 to 3, wherein a guide portion for guiding movement of the rod-shaped portion of the movable member is provided on a side wall portion of the casing. .   The housing includes a light shielding sheet storage for storing the light shielding sheet between the space between the light source and the light transmitting plate and a base end portion of the light shielding sheet. The transilluminator for electrophoretic gels according to any one of claims 1 to 4.   The transilluminator for electrophoretic gel according to any one of claims 1 to 5, wherein the light shielding sheet is bent into a corrugated shape and can be freely extended and folded.

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