JP2010014586A5 - - Google Patents

Download PDF

Info

Publication number
JP2010014586A5
JP2010014586A5 JP2008175668A JP2008175668A JP2010014586A5 JP 2010014586 A5 JP2010014586 A5 JP 2010014586A5 JP 2008175668 A JP2008175668 A JP 2008175668A JP 2008175668 A JP2008175668 A JP 2008175668A JP 2010014586 A5 JP2010014586 A5 JP 2010014586A5
Authority
JP
Japan
Prior art keywords
antibody
hemoglobin
measurement method
carrier
haptoglobin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2008175668A
Other languages
Japanese (ja)
Other versions
JP5191291B2 (en
JP2010014586A (en
Filing date
Publication date
Application filed filed Critical
Priority to JP2008175668A priority Critical patent/JP5191291B2/en
Priority claimed from JP2008175668A external-priority patent/JP5191291B2/en
Publication of JP2010014586A publication Critical patent/JP2010014586A/en
Publication of JP2010014586A5 publication Critical patent/JP2010014586A5/ja
Application granted granted Critical
Publication of JP5191291B2 publication Critical patent/JP5191291B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Claims (14)

便検体中のヘモグロビンを測定する免疫学的測定方法であって、抗ハプトグロビン抗体を反応系中に共存させることを特徴とする、便検体中のヘモグロビンの測定方法。 A method for measuring hemoglobin in a stool specimen, which is an immunological measurement method for measuring hemoglobin in a stool specimen, wherein an anti-haptoglobin antibody coexists in a reaction system. 免疫学的測定方法が、抗ヘモグロビン抗体を担持した担体と便検体抽出液とを反応させて生じる凝集反応に由来する光学的変化に基づく測定方法である、請求項１記載の測定方法。 The measurement method according to claim 1, wherein the immunological measurement method is a measurement method based on an optical change derived from an agglutination reaction caused by reacting a carrier carrying an anti-hemoglobin antibody with a stool specimen extract. 光学的変化が、抗ヘモグロビン抗体を担持した担体とヘモグロビンの反応開始後、反応液の光学的測定を適当な間隔で２回行った時の測定値の差、又は抗ヘモグロビン抗体を担持した担体とヘモグロビンとの反応開始後の反応液の光学的変化率である、請求項２に記載の測定方法。The optical change is the difference between the measured value when the optical measurement of the reaction solution is performed twice at an appropriate interval after the reaction between the carrier carrying the anti-hemoglobin antibody and the hemoglobin starts, or the carrier carrying the anti-hemoglobin antibody. The measuring method according to claim 2, which is an optical change rate of the reaction solution after the start of the reaction with hemoglobin. 担体が金コロイド又はラテックス粒子である請求項２又は３に記載の測定方法。 The measuring method according to claim 2 or 3, wherein the carrier is gold colloid or latex particles. 担体が金コロイドである請求項２又は３に記載の測定方法。 The measurement method according to claim 2 or 3, wherein the carrier is a gold colloid. 抗ヘモグロビン抗体がモノクローナル抗体である請求項２〜５の何れかに記載の測定方法。 Measurement method according to any of claims 2-5 an anti-hemoglobin antibody is a monoclonal antibody. 抗ハプトグロビン抗体がポリクローナル抗体である請求項２〜６の何れかに記載の測定方法。 The measurement method according to any one of claims 2 to 6 , wherein the anti-haptoglobin antibody is a polyclonal antibody. 光学的変化が吸光度変化である、請求項２〜７の何れかに記載の測定方法。 The measurement method according to claim 2, wherein the optical change is a change in absorbance. 光学的変化が500〜550nmの吸光度変化である、請求項５に記載の測定方法。 The measurement method according to claim 5, wherein the optical change is an absorbance change of 500 to 550 nm. 抗ヘモグロビン抗体を担持した担体を含有する試薬及び抗ハプトグロビン抗体を含有する試薬を含んでなる、或いは抗ヘモグロビン抗体を担持した担体及び抗ハプトグロビン抗体を含有する試薬を含んでなる、便検体中のヘモグロビン測定用試薬キット。 Hemoglobin in a stool specimen comprising a reagent containing a carrier carrying an anti-hemoglobin antibody and a reagent containing an anti-haptoglobin antibody, or comprising a carrier carrying an anti-hemoglobin antibody and a reagent containing an anti-haptoglobin antibody Reagent kit for measurement. 担体が金コロイド又はラテックス粒子である請求項１０記載のキット。 The kit according to claim 10, wherein the carrier is colloidal gold or latex particles. 担体が金コロイドである請求項１１記載のキット。 The kit according to claim 11, wherein the carrier is a colloidal gold. 抗ヘモグロビン抗体がモノクローナル抗体である請求項１０〜１２の何れかに記載のキット。 The kit according to any one of claims 10 to 12, wherein the anti-hemoglobin antibody is a monoclonal antibody. 抗ハプトグロビン抗体がポリクローナル抗体である請求項１０〜１３の何れかに記載のキット。 The kit according to any one of claims 10 to 13, wherein the anti-haptoglobin antibody is a polyclonal antibody.
JP2008175668A 2008-07-04 2008-07-04 Method and reagent kit for measuring hemoglobin in stool specimen Active JP5191291B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008175668A JP5191291B2 (en) 2008-07-04 2008-07-04 Method and reagent kit for measuring hemoglobin in stool specimen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2008175668A JP5191291B2 (en) 2008-07-04 2008-07-04 Method and reagent kit for measuring hemoglobin in stool specimen

Publications (3)

Publication Number Publication Date
JP2010014586A JP2010014586A (en) 2010-01-21
JP2010014586A5 true JP2010014586A5 (en) 2011-07-21
JP5191291B2 JP5191291B2 (en) 2013-05-08

Family

ID=41700826

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008175668A Active JP5191291B2 (en) 2008-07-04 2008-07-04 Method and reagent kit for measuring hemoglobin in stool specimen

Country Status (1)

Country Link
JP (1) JP5191291B2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5877150B2 (en) * 2010-03-31 2016-03-02 積水メディカル株式会社 Method for producing conjugate for measuring multivalent antigen
JP5861977B2 (en) * 2014-06-18 2016-02-16 関根　弘一 Fecal color detection device
WO2017209001A1 (en) * 2016-05-30 2017-12-07 栄研化学株式会社 Anti-human hemoglobin monoclonal antibody or antibody kit, insoluble carrier particle to which anti-human hemoglobin monoclonal antibody is immobilized, and measurement reagent and measurement method using same
SG11202102265UA (en) * 2018-09-26 2021-04-29 Eiken Chemical Hemoglobin assay reagent, assay kit and assay method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02193071A (en) * 1989-01-20 1990-07-30 Green Cross Corp:The Kit of reagent for measuring haptoglobin-hemoglobin complex and measuring method of haptoglobin-hemoglobin complex using the same
JPH07103978A (en) * 1993-09-30 1995-04-21 Wakamoto Pharmaceut Co Ltd Measurement of free hemoglobin
JPH10132824A (en) * 1996-10-28 1998-05-22 Eiken Chem Co Ltd Hemoglobin stabilizing method
JPH11201969A (en) * 1998-01-14 1999-07-30 Wakamoto Pharmaceut Co Ltd Simple free hemoglobin measuring method and kit for measurement
JP4261665B2 (en) * 1999-03-05 2009-04-30 アルフレッサファーマ株式会社 Method for stabilizing human hemoglobin in solution and stabilizing solution

Similar Documents

Publication Publication Date Title
Oh et al. Vertical flow immunoassay (VFA) biosensor for a rapid one-step immunoassay
Yamaguchi et al. Immunosensor with fluid control mechanism for salivary cortisol analysis
JP2012073273A5 (en)
JP5855246B2 (en) Determination of active concentration by calibration-free analysis
JP2010014586A5 (en)
WO2006083367A3 (en) Immunoassay employing two-step internal calibration reaction
JP2015523054A5 (en)
JP2012509477A5 (en)
JP2014508298A5 (en)
JP2008185527A5 (en)
JP2017508971A5 (en)
JP2015531487A5 (en)
JP2017517753A5 (en)
WO2010150254A3 (en) A method and system for the detection of cancer
JP2022033935A5 (en)
JP2013174612A5 (en)
EP1801590A4 (en) Method of assaying antigen and reagent therefor
SG157244A1 (en) Point of care test for the detection of exposure or immunity to dengue virus
ATE496300T1 (en) IMMUNE TEST METHOD AND CHIP
Glass et al. Least detectable concentration and dynamic range of three immunoassay systems using the same antibody
CN201045616Y (en) Clip type mycoplasma pneumonia antibody detecting reagent box
JP2008527372A5 (en)
WO2011159741A3 (en) Tetranor-pgem/pgam specific immunogens, antibodies, tracers, assay kits and methods for making same
CN107436360A (en) A kind of small-sized ELISA detecting systems of GP73 and application method
JP2022033286A5 (en)