JP2010002395A - Reaction plate and automatic analyzer - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an automatic analyzer capable of reducing an analysis time and the work of an analytical process, in an analytical process for liquid-feeding a reagent or a specimen to a reaction cell by centrifugal force. <P>SOLUTION: The automatic analyzer 1, comprising a reaction plate 20 equipped with a dispensing cell, the reaction cell, and a transfer passage for connecting the cells and a rotor 11 holding the reaction plate 20: includes a barrier in the transfer passage on the reaction plate 20 and a rotation controller 32 for controlling the rotation speed; and can make the reagent and the specimen react gradually by varying the rotation speed. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a reaction plate used for analyzing a sample component by reacting a sample and a reagent in a reaction vessel and optically measuring the result of the reaction, and an automatic analyzer using the reaction plate. is there.

  Conventionally, as a device that automatically analyzes specimens such as blood and body fluids, the specimen and reagent are dispensed into a reaction container, the specimen and reagent are reacted, and then the absorbance of the reaction liquid of this specimen and reagent is measured. Thus, an analyzer that automatically analyzes a sample is known. In addition, a sample and a reagent or a mixed solution of a sample and a reagent are dispensed into a dispensing cell of a reaction plate arranged on a rotating body and connected between cells by a flow path, and the rotating body is rotated. A liquid-feeding device and a liquid-feeding method that control the liquid-feeding of a reaction liquid of a specimen and a reagent using the generated centrifugal force are disclosed (for example, see Patent Document 1).

JP 2007-330857 A

  However, in the liquid feeding method shown in Patent Document 1, since the specimen and the reagent are dispensed into one cell, when reacting stepwise, it is necessary to repeat dispensing or dispensing and rotation. It took time and effort. In addition, if the barrier is disposed in the direction of centrifugal force, it is difficult to perform a continuous step reaction. Furthermore, if a reagent is arranged between the flow paths, it is difficult to secure a reaction time, which may affect analysis accuracy.

  The present invention has been made in view of the above, and provides a reaction plate capable of shortening the working time by adjusting the feeding of a specimen and a reagent to a cell, and an automatic analyzer using the reaction plate The purpose is to do.

  In order to solve the above-described problems and achieve the object, a reaction plate according to the present invention has a plurality of cells and a transfer flow path connecting the cells, and dispenses a specimen and a reagent to a predetermined cell, respectively. Then, in the reaction plate that causes the sample and the reagent to flow through the transfer channel by the centrifugal force obtained from the rotation of the rotating body, the barrier is provided in the transfer channel, and the sample, the reagent, or the reaction It is characterized by controlling the flow of the liquid.

  In the reaction plate according to the present invention as set forth in the invention described above, the barriers have different heights.

  In the reaction plate according to the present invention as set forth in the invention described above, the barrier is formed of a hydrophobic protrusion.

  In the reaction plate according to the present invention as set forth in the invention described above, the height of the barrier increases according to the order in which the reactions are performed.

  Further, in the automatic analyzer according to the present invention, a reaction plate that forms a plurality of cells and a transfer channel that connects the cells, a barrier that controls the flow of a sample, a reagent, or a reaction solution in the transfer channel; A rotating body that holds the reaction plate, and a rotation control unit that controls the flow of the specimen, the reagent, or the reaction liquid through the transfer channel by rotation of the rotating body.

  The automatic analyzer according to the present invention is characterized in that, in the above invention, the rotation control means controls the flow of the sample, the reagent or the reaction solution by changing a rotation speed of the rotating body. To do.

  Further, the automatic analyzer according to the present invention includes a moving means for moving the reaction plate on the rotating body in a radial direction in the above invention, and the difference in angular velocity that the reaction plate receives by moving the position is determined. The flow of the sample, the reagent, or the reaction liquid in the transfer channel is controlled by using.

  The automatic analyzer according to the present invention further includes a dispensing mechanism for dispensing the sample and the reagent in the above-described invention, and the reaction of the sample and the reagent before the rotating body starts rotating. Dispensing into predetermined cells of the plate.

  According to the present invention, a barrier is provided in the flow path connecting cells formed in the reaction plate, and the rotation control unit that controls the rotation of the rotating body that holds the reaction plate is provided. Since all the reagents to be used can be dispensed and fed in stages by controlling the rotation, there is no need to repeat the rotation stop and dispensing, and the analysis processing time can be shortened. Moreover, since the transfer of the solution from the cell to the cell can be adjusted by rotation control, it is possible to cope with a multistage reaction.

  Hereinafter, an automatic analyzer which is the best mode for carrying out the present invention will be described with reference to the drawings. In addition, this invention is not limited by each embodiment. In the description of the drawings, the same parts are denoted by the same reference numerals.

(Embodiment 1)
FIG. 1 is a schematic diagram illustrating a configuration of an automatic analyzer 1 according to the embodiment. As shown in FIG. 1, the automatic analyzer 1 dispenses a specimen and a reagent to a reaction plate 20, and optically measures a reaction occurring in the dispensed reaction plate 20, and the measurement mechanism 10. And a control mechanism 30 that analyzes the measurement result in the measurement mechanism 10 and controls the entire automatic analyzer 1 including The automatic analyzer 1 automatically performs biochemical, immunological or genetic analysis of a plurality of specimens by cooperation of these two mechanisms.

  The measurement mechanism 10 includes a rotator 11, a photometric unit 12, reagent dispensing units 13 and 14, a sample transfer unit 17, and a sample dispensing unit 18. The control mechanism 30 includes a control unit 31, a rotation control unit 32, a dispensing control unit 33, an input unit 34, an analysis unit 35, a storage unit 36, and an output unit 37. These units included in the measurement mechanism 10 and the control mechanism 30 are electrically connected to the control unit 31.

  The rotating body 11 is rotatable about a vertical line passing through the center of the rotating body 11 as a rotation axis when driven by a drive mechanism (not shown). It is preferable to provide an openable / closable lid and a temperature adjusting mechanism (not shown) above and below the rotating body 11.

  The photometric unit 12 receives the light transmitted through the sample in the reaction plate 20 conveyed to a predetermined photometric position and measures the intensity. The measurement result by the photometry unit 12 is output to the control unit 31 and analyzed by the analysis unit 35. Moreover, you may have a photomultiplier tube which measures the weak light which the reaction liquid in the reaction plate 20 light-emits. In addition, when measuring the fluorescence generated from the reaction solution, the photometry unit 12 may be provided with a light source for irradiating excitation light.

  The reagent dispensing units 13 and 14 include arms 13a and 14a that freely move up and down in the vertical direction and rotate around a vertical line passing through the base end of the reagent dispensing unit 13 and 14, respectively. Probes for aspirating and discharging the specimen are attached to the distal ends of the arms 13a and 14a. The reagent dispensing units 13 and 14 include an intake / exhaust mechanism using an unillustrated intake / exhaust syringe or piezoelectric element. The reagent dispensing units 13 and 14 suck the sample from the reagent containers 15a and 16a by the probe, rotate the arms 13a and 14a in the direction of the arrow in the figure, and discharge the sample to a predetermined cell of the reaction plate 20. Do dispensing.

  The reagent containers 15 and 16 can store a plurality of reagent containers 15 a and 16 a in which reagents to be dispensed in the reaction plate 20 are stored. A plurality of storage chambers are arranged at equal intervals in the reagent storages 15 and 16, and reagent containers 15a and 16a are detachably stored in the storage chambers. Under the control of the control unit 31, the reagent storages 15 and 16 are driven clockwise or counterclockwise around a vertical line passing through the center of each of the reagent storages 15 and 16 as a driving mechanism (not shown) is driven. It is rotatable, and the desired reagent containers 15a, 16a are transferred to the reagent aspirating position by the reagent dispensing units 13, 14. An openable / closable lid (not shown) is provided above the reagent containers 15 and 16. In addition, a cold storage tank is provided below the reagent containers 15 and 16. Therefore, when the reagent containers 15a and 16a are stored in the reagent containers 15 and 16 and the lid is closed, the reagent stored in the reagent container 15a is kept at a constant temperature state, and the reagent containers 15a and 16a Evaporation and denaturation of the reagent contained in the container can be suppressed.

  The sample transfer unit 17 includes a plurality of sample racks 17b that hold a plurality of sample containers 17a that store liquid samples such as blood and urine, and sequentially transfer them in the direction of the arrows in the figure. The sample in the sample container 17 a transferred to a predetermined position on the sample transfer unit 17 is dispensed by the sample dispensing unit 18 to the reaction plate 20 conveyed to a predetermined position on the rotating body 11.

  Similar to the reagent dispensing units 13 and 14, the sample dispensing unit 18 includes an arm 18 a to which a probe for aspirating and discharging a sample is attached to the tip. The arm 18a freely moves up and down in the vertical direction and rotates around a vertical line passing through its base end as a central axis. The sample dispensing unit 18 sucks the reagent in the sample container 17a with a probe, rotates the arm 18a in the direction of the arrow in the figure, and dispenses it to a predetermined cell of the reaction plate 20 conveyed to a predetermined position on the rotating body 11. Note.

  The reaction plate 20 is an optically transparent material that transmits 80% or more of the light contained in the analysis light (340 to 800 nm) emitted from the light source, such as glass containing heat-resistant glass, cyclic olefin, polystyrene, or the like. A plate made of resin is used. Reagents are dispensed from the reagent containers 15a and 16a of the reagent containers 15 and 16 to the predetermined cells formed on the reaction plate 20 by the reagent dispensing units 13 and 14 which are operating units provided in the vicinity. The part 18 dispenses the sample from the sample container 17a of the sample rack 17b.

  Next, the control mechanism 30 will be described. The control unit 31 is configured using a CPU or the like, and controls processing and operation of each unit of the automatic analyzer 1. The control unit 31 performs predetermined input / output control on information input / output to / from each of these components, and performs predetermined information processing on this information. The control unit 31 includes a rotation control unit 32 and a dispensing control unit 33. The rotation control unit 32 controls the rotation start and stop or the rotation speed of the rotating body 11. The dispensing control unit 33 controls the dispensing of the specimen and the reagent.

  The input unit 34 is configured by using a keyboard, a mouse, and the like, and acquires various information necessary for analyzing a sample, instruction information for analysis operation, and the like from the outside. The analysis unit 35 calculates absorbance or luminescence intensity based on the measurement result acquired from the photometry unit 12, and performs component analysis of the specimen. The storage unit 36 is configured using a hard disk that magnetically stores information and a memory that loads various programs related to the process from the hard disk and electrically stores them when the automatic analyzer 1 executes the process. Various information including the analysis result of the specimen is stored. The storage unit 36 may include an auxiliary storage device that can read information stored in a storage medium such as a CD-ROM, a DVD-ROM, or a PC card. The output unit 37 is configured using a printer, a communication mechanism, and the like, and outputs various information including the analysis result of the sample.

  In the automatic analyzer 1 configured as described above, the sample dispensing unit 18 dispenses the sample in the sample container 17a with respect to the reaction plate 20, and the reagent dispensing units 13 and 14 include the reagent containers 15a and 16a. After dispensing the reagent in the sample, the sample is reacted with the reagent, the photometry unit 12 performs optical measurement of the reaction solution, and the analysis result is analyzed by the analysis unit 35 to automatically analyze the component of the sample. To be done. This series of analysis operations is repeated continuously.

  Next, the reaction plate 20 according to the first embodiment will be described in detail with reference to FIG. FIG. 2 is a schematic view of the reaction plate 20 as viewed from above, in which dispensing cells 21, 22, 24 and reaction cells 23, 25 are formed. The cells are connected by transfer channels 21a, 22a, 23a, and 24a, and barriers 21b, 22b, 23b, and 24b are formed in the transfer channels. Here, the heights of the barriers 21b and 22b are set so that the reagent and the sample dispensed in the dispensing cells 21 and 22 can simultaneously overcome the barriers 21b and 22b by the centrifugal force applied by the rotation of the rotating body, The barriers 23b and 24b are set so that the reagent and the reaction solution can get over the barriers 23b and 24b when the speed is increased by one step without being overcome at the rotational speed described above. In addition, in order to provide a barrier in the transfer flow path having an angle different from the centrifugal force direction, it is necessary to set the height of the barrier in consideration of the angle and force applied to the barrier.

  In the analysis process, first, the first reagent is dispensed into the dispensing cell 21, the sample is dispensed into the dispensing cell 22, and the second reagent is dispensed into the dispensing cell 24 (FIG. 2A). Thereafter, the first reagent and the sample flow through the transfer channels 21a and 22a and flow into the reaction cell 23 by the centrifugal force generated by the rotation control unit 32 rotating the rotator 11 (FIG. 2B). Note that the centrifugal force applied to the reaction plate 20 is vertically downward in FIG.

  Next, the rotation speed of the rotating body 11 is increased by the rotation control unit 32, and the first reaction liquid in which the first reagent reacts with the sample passes through the transfer channel 23a and the barrier 23b from the reaction cell 23 and reacts. It flows into the cell 25. Here, since the second reagent dispensed in the dispensing cell 24 also passes through the transfer flow path 24a and the barrier 24b and flows into the reaction cell 25, the second reagent and the reaction liquid are mixed and reacted. . This second reaction liquid is accommodated in the reaction cell 25, and the photometric unit 12 performs optical measurement (FIG. 2C).

  Subsequently, the shapes of the cell, the transfer flow path, and the barrier of the reaction plate 20 will be described with reference to FIGS. 3 shows a cross-sectional view taken along the line AA in FIG. 2, and FIG. 4 shows a cross-sectional view taken along the line BB in FIG.

  In FIG. 3, the specimen or reagent dispensed into the dispensing cell 22 is prevented from flowing out into the reaction cell 23 by the barrier 22b even when vibration occurs due to an external factor or the like. Similarly, in FIG. 4, the liquid is prevented from flowing out by the barrier 24b. Further, the barriers 22b and 24b have different heights, and liquid feeding can be performed in stages according to the rotational speed of the rotating body 11 by making different centrifugal forces that allow the solution to get over the barrier. Further, by giving a height difference to the dispensing cell 22 and the reaction cell 23 and the transfer flow path 22a, and forming the bottom of the dispensing cell 22 and the reaction cell 23 in a circular shape, by centrifugal force or external force Even when the reagent or the reaction liquid flows into the transfer channel 22a, the unreacted reagent does not remain in the transfer channel 22a by aggregating at the cell bottom due to the difference in height.

  FIG. 5 shows a flowchart relating to the analysis processing of the first embodiment, and a series of analysis processing will be described using this flowchart.

  First, when receiving an analysis instruction, the control unit 31 instructs the sample dispensing unit 18 and the reagent dispensing units 13 and 14 to dispense the sample and the reagent into a predetermined dispensing cell. (Step S102). When the dispensing of the sample and the reagent is completed, the control unit 31 instructs the rotation control unit 32 to rotate the rotating body 11 at the first speed (step S104). After a predetermined time has elapsed, the rotation control unit 32 rotates the rotating body 11 at the second speed (step S106). Thereafter, the photometric unit 12 measures the optical characteristics of the reaction solution stored in the reaction cell of the reaction plate (step S108). After the photometry process is completed, if there is an instruction to analyze the next sample in the control unit 31 (step S110: Yes), the process proceeds to step S102 and the analysis process for the next sample is performed. If there is no analysis instruction for the next sample (step S110: No), the analysis process is terminated. The sample is analyzed by repeatedly performing the analysis process described above.

  The barrier of the reaction plate 20 of the present invention is preferably hydrophobic. FIG. 6 shows a case where the volume of the reagent or specimen stored in the cell is large in the cross section taken along the line AA of FIG. Even when the liquid level is higher than the barrier 22b, the barrier 22b is made hydrophobic, so that it is possible to prevent the reagent or specimen from flowing out to the reaction cell 23 due to the surface tension of the reagent. Moreover, even when liquid enters the transfer channel due to capillary action, outflow can be prevented by making the barrier a hydrophobic wall. Furthermore, in order to efficiently send the liquid to the reaction cell, it is preferable to make the surface of the dispensing cell and the reaction cell and the transfer channel hydrophilic so as to improve the fluidity of the liquid. Here, when the material used for the reaction plate 20 is hydrophilic, the barrier may be coated with a water repellent or the like.

  The reaction plate of the present invention can also be used for analysis requiring a reaction process of three or more stages, such as immunoassay using a fluorescent substance or an enzyme. FIG. 7 shows a schematic diagram of a reaction plate 40 used for immunoassay. Dispensing cells 41, 42, 44, 46 and reaction cells 43, 45, 47 are formed in the same manner as the reaction plate 20. By setting the speed in three stages and setting the height of the barrier from each centrifugal force, a three-stage reaction becomes possible. First, the rotation control unit 32 is set so as to get over the barriers 41 b and 42 b at the first speed, and causes the specimen and the first reagent to flow through the first reaction cell 43 through the transfer channels 41 a and 42 a. Thereafter, the rotation control unit 32 rotates the rotator 11 at the second speed to pass the first reaction liquid and the second reagent through the barriers 43b and 44b to the second reaction cell 45 from the transfer channels 43a and 44a. And send. Finally, the rotation control unit 32 rotates the rotator 11 at the third speed, so that the second reaction liquid and the substrate or enzyme pass through the barriers 45b and 46b and the transfer channels 45a and 46a, thereby causing the reaction cell 47. Send to. After performing the third reaction, the optical characteristics are measured by the photometric unit 12.

  Here, in the conventional automatic analyzer, since the transfer channel is not provided with a barrier, all the solutions flow out simultaneously when a centrifugal force is applied. Therefore, in a multistage reaction, the rotation is stopped for each reaction, and the reagent used for the next reaction is dispensed.

  On the other hand, in the reaction plate and the automatic analyzer according to the first embodiment, it is possible to control the liquid feeding by providing a barrier in the transfer channel, and the sample used before rotating the rotating body and All reagents can be dispensed. As a result, it is not necessary to stop the rotation and dispense the reagent, thereby shortening the analysis time and reducing the labor required for the analysis process. In addition, it is possible to cope with multistage reactions by forming cells on the reaction plate.

  In the reaction plate according to the first embodiment, the order in which the liquids are fed is such that if the reagents are mixed in the order of the reaction, the second and third reagents are first allowed to flow out to a predetermined reaction cell. Anyway. Further, when the rotating body 11 is stopped due to the volume of the reagent, if the reagent does not flow out from the dispensing cell to the reaction cell, the barrier may be extremely low, but the solution outflow due to capillary action is considered. Thus, it is preferable to provide a hydrophobic barrier at least. The barrier may have any shape as long as the solution does not spontaneously flow out to the next cell, and a valve or a membrane may be used instead of a protrusion. Further, when the liquid is sent by applying centrifugal force, if there is a possibility that the liquid may scatter, it may be prevented by a lid or the like, or the cell and the transfer channel may be formed in the plate. Furthermore, in order to improve the analysis efficiency, a plurality of reaction plates 20 may be installed on the rotating body 11 for analysis.

(Embodiment 2)
FIG. 8 is a schematic diagram illustrating a configuration of the automatic analyzer 1 according to the second embodiment. As in the first embodiment, as shown in FIG. 8, the automatic analyzer 1 dispenses a sample and a reagent to the reaction plate 20 and optically measures the reaction occurring in the dispensed reaction plate 20. 10 and a control mechanism 30 that controls the entire automatic analyzer 1 including the measurement mechanism 10 and analyzes a measurement result in the measurement mechanism 10.

  In the second embodiment, by adjusting the position of the reaction plate 20 without changing the speed of the rotating body 11, the liquid feeding is adjusted by the difference in the angular velocity that the reaction plate 20 receives from the rotation of the rotating body. As shown in FIG. 8, the reaction plate 20 is held by the plate holding unit 19, and the movement control unit 38 moves the position by moving the reaction plate 20 in the plate holding unit 19 in the radial direction.

  FIG. 9 is a schematic diagram showing the plate holding unit 19 and the reaction plate 20. The holder 19a holds the reaction plate 20 extending in the radial direction, and is placed in the holder stacking hole 19b. Further, a convex portion is formed on the holder 19a so that the reaction plate 20 does not slide outwardly due to centrifugal force. The holder 19a slides by driving a drive unit (not shown) by the movement control unit 38, and moves the position of the reaction plate 20.

  FIG. 10 shows a flowchart relating to the analysis processing of the second embodiment, and a series of analysis processing will be described using this flowchart.

  First, when receiving an analysis instruction, the control unit 31 instructs the sample dispensing unit 18 and the reagent dispensing units 13 and 14 to dispense the sample and the reagent into a predetermined dispensing cell. (Step S202). When the dispensing of the sample and the reagent is completed, the control unit 31 instructs the movement control unit 38 to move the holder 19a and place the reaction plate at the first rotation position (step S204). Next, the control unit 31 instructs the rotation control unit 32 to rotate the rotating body 11 (step S206). After a predetermined time has elapsed, the rotation control unit 32 stops the rotating body 11 (step S208), and the movement control unit 38 moves the holder 19a to place the reaction plate 20 at the second rotation position (step S210). When the reaction plate 20 is disposed at the second rotation position, the rotation control unit 32 rotates the rotating body 11 (step S212), and stops rotating after a predetermined time has elapsed (step S214). Thereafter, the photometric unit 12 measures the optical characteristics of the reaction solution stored in the reaction cell of the reaction plate 20 (step S216). After the photometric process is completed, if there is an instruction to analyze the next sample in the control unit 31 (step S218: Yes), the process proceeds to step S202 to perform an analysis process for the next sample. If there is no analysis instruction for the next sample (step S218: No), the analysis process is terminated. The sample is analyzed by repeatedly performing the analysis process described above.

  Note that the rotation speed of the rotating body 11 according to the second embodiment may be changed as long as the liquid feeding timing can be controlled. Moreover, when performing reaction of 3 steps | paragraphs or more, even if the movement of the holder 19a is set to 3 steps | paragraphs, you may carry out liquid feeding control of the reaction process after the 3rd time with a rotational speed.

  Here, in the automatic analyzer according to the second embodiment, similarly to the first embodiment, it is possible to control liquid feeding by providing a barrier in the transfer flow path, and before rotating the rotating body. All specimens and reagents used can be dispensed. As a result, it is not necessary to stop the rotation and dispense the reagent, thereby shortening the analysis time and reducing the labor required for the analysis process. In addition, the liquid feeding can be adjusted with easy control by keeping the speed constant.

  In the first and second embodiments described above, when stirring is required, a stirring mechanism such as a vibrator, a sound wave, or an ultrasonic wave may be provided.

  Further, the present invention is not limited to Embodiments 1 and 2 described above, and can be applied to, for example, an automatic analyzer that performs genetic analysis of a specimen. That is, the present invention can include various embodiments and the like not described herein, and various design changes and the like can be made without departing from the technical idea specified by the claims. Is possible.

1 is a schematic diagram illustrating an automatic analyzer according to a first embodiment. It is a schematic diagram explaining the liquid feeding in a reaction plate. It is the sectional view on the AA line of FIG. FIG. 3 is a sectional view taken along line B-B in FIG. 2. 3 is a flowchart illustrating analysis processing according to the first embodiment. FIG. 3 is a cross-sectional view taken along the line AA of FIG. 2 and showing a case where a large amount of solution is stored in the dispensing cell. It is a schematic diagram which shows the reaction plate applied to the immunoassay. FIG. 3 is a schematic diagram showing an automatic analyzer according to a second embodiment. It is a schematic diagram which shows a reaction plate and a plate holding | maintenance part. 10 is a flowchart illustrating analysis processing according to the second embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Automatic analyzer 10 Measurement mechanism 11 Rotating body 12 Photometry part 13,14 Reagent dispensing part 15,16 Reagent storage 15a, 16a Reagent container 17 Specimen transfer part 17a Specimen container 17b Specimen rack 18 Specimen dispensing part 19 Plate holding part 19a Holder 20, 40 Reaction plate 30 Control mechanism 31 Control unit 32 Rotation control unit 33 Dispensing control unit 34 Input unit 35 Analysis unit 36 Storage unit 37 Output unit 38 Movement control unit

Claims (8)

A plurality of cells and a transfer flow path connecting the cells, each of the sample and the reagent being dispensed into a predetermined cell, and the transfer of the sample and the reagent by centrifugal force obtained from rotation of a rotating body. In the reaction plate that is circulated and reacted in the road,
A reaction plate, wherein a barrier is provided in the transfer flow path to control the flow of the specimen, the reagent, or the reaction liquid.   The reaction plate according to claim 1, wherein the barriers have different heights.   The reaction plate according to claim 1, wherein the barrier is formed of a hydrophobic protrusion.   The reaction plate according to any one of claims 1 to 3, wherein the height of the barrier increases according to the order in which the reactions are performed. A reaction plate that forms a plurality of cells and a transfer channel that connects the cells, and a barrier that controls the flow of a sample, a reagent, or a reaction solution in the transfer channel;
A rotating body for holding the reaction plate;
Rotation control means for controlling the flow of the specimen, the reagent or the reaction liquid in the transfer channel by rotation of the rotating body;
An automatic analyzer characterized by comprising:   6. The automatic analyzer according to claim 5, wherein the rotation control unit controls the flow of the specimen, the reagent, or the reaction liquid by changing a rotation speed of the rotating body. A moving means for moving the reaction plate on the rotating body in a radial direction;
6. The automatic analysis according to claim 5, wherein the flow of the sample, the reagent, or the reaction liquid in the transfer flow path is controlled using a difference in angular velocity that the reaction plate receives by moving the position. apparatus. A dispensing mechanism for dispensing the sample and the reagent;
The automatic analyzer according to any one of claims 5 to 7, wherein the sample and the reagent are dispensed into a predetermined cell of the reaction plate before the rotating body starts rotating.

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