JP2009296914A - Cardiopulmonary function-improving composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cardiopulonary function-improving composition which is safe even when taken for a long period of time, improves cardiopulmonary function in a short time by oral intake before exercise, and holds promise in exercise performance-improving effect. <P>SOLUTION: The cardiopulmonary function-improving composition contains an egg albumen hydrolysate and organic acid as its active ingredients. The cardiopulmonary function-improving composition preferably contains 0.2-1.2 pts.mass of the organic acid based on 1 pt.mass of the egg albumen hydrolysate (solid). The organic acid preferably is citric acid. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a composition that can be expected to improve cardiopulmonary function.

  The heart rate that is close to the heart rate at the end of exercise is called the recovery heart rate. If the heart rate is 120 beats / minute or more 5 minutes after the end of exercise, exercise is strong against the physical condition at that time. It is said that it is too much. People who are training regularly are known to recover this heart rate faster, and as a result, they can adapt to high exercise intensity. For example, in many sports, there is a short interval during the competition time, but how much fatigue can be recovered during that interval also determines the victory or defeat, so the heart rate is recovered quickly. This is considered significant.

  In general, it is said that the decrease in performance during exercise is caused by accumulation of fatigue substances, lack of energy sources, loss of moisture and minerals due to sweating, and the like. Therefore, many foods and drinks have been developed for the purpose of reducing fatigue substances, supplementing sugars and vitamins, which are energy sources, and moisture and minerals.

  For example, Patent Document 1 discloses a sports beverage to which a predetermined amount of citric acid is added, and describes that the effect of citric acid can reduce the lactic acid level and promote fatigue recovery.

In addition, the present applicant has found that egg white peptides have an inhibitory effect on the increase in blood lactic acid level and an inhibitory effect on the increase in deep body temperature. In Patent Document 2, an egg white peptide obtained by hydrolyzing egg white is used as an active ingredient. Disclosed is a composition for suppressing an increase in blood lactic acid level, characterized by containing, and in Patent Document 3, containing egg white peptide obtained by hydrolyzing egg white as an active ingredient The beverage is disclosed.
JP 2006-42602 A JP 2006-273850 A JP 2008-72968 A

  However, many of the conventional foods and drinks as described above are used for coping therapy, and are aimed at maintaining exercise performance rather than improving exercise performance.

  On the other hand, the improvement of the cardiopulmonary function leads to the improvement of the exercise performance, but it can be obtained only by daily training, and it is usually difficult to improve the cardiopulmonary function within a short period of time.

  Therefore, an object of the present invention is to provide a composition that can be safely taken for a long period of time and can improve cardiopulmonary function in a short time by taking it orally before exercise, and can be expected to improve exercise performance. It is in.

  That is, the composition for improving cardiopulmonary function of the present invention is characterized by containing an egg white hydrolyzate and an organic acid as active ingredients.

  In the composition for improving cardiopulmonary function of the present invention, the organic acid is preferably contained in an amount of 0.2 to 1.2 parts by mass with respect to 1 part by mass of the egg white hydrolyzate (solid content), and the organic acid is citric acid. Is preferred.

  The composition for improving cardiopulmonary function of the present invention can improve cardiopulmonary function and accelerate recovery of heart rate after exercise by the synergistic effect of egg white hydrolyzate and organic acid. As a result, it is possible to recover from fatigue even during a short interval during the competition time, and an improvement in exercise performance can be expected.

  According to the present invention, it is possible to provide a composition that can be expected to improve exercise performance by improving cardiopulmonary function, speeding up recovery of heart rate after exercise, and taking exercise orally. Egg whites and organic acids, which are the active ingredients of this composition, are safe ingredients that have been eaten for a long time, so there is no concern about side effects and they can be taken continuously.

  Hereinafter, the raw material of the composition for improving cardiopulmonary function of the present invention will be described.

  In the present invention, the egg white hydrolyzate used as an active ingredient is an egg white peptide composition obtained by hydrolyzing egg white using an acid or an enzyme, and preferably obtained by enzymatic decomposition of egg white. .

  The raw egg white is, for example, egg white of chicken, quail, duck, goose, etc., but egg white of chicken eggs that are easily available in large quantities is preferred, egg white liquid, frozen egg white liquid, egg white powder, etc. can be used, In particular, those subjected to desugaring treatment are preferably used.

  For example, the egg white hydrolyzate is prepared by adding hydrolase to 1 part by weight of liquid egg white and adding 0.1 to 1 part by weight of water and stirring the enzyme, followed by an enzyme reaction for a predetermined time, and then subjecting the enzyme to inactivation. It can be obtained by separating the liquid and recovering the liquid part and making it into dry powder. If necessary, fractionation and purification may be performed using an ultrafiltration membrane, a reverse osmosis membrane, gel filtration, various column chromatography, a membrane filter, or the like.

  As the hydrolase, a protein hydrolase having protease activity or peptidase activity and usable for food production is used. Specifically, papain (EC.3.4.22.2), trypsin (EC.3.4.21.4 ), Protease derived from Bacillus bacteria (for example, trade name “Protease S“ Amano ”G”, trade name “Protease N“ Amano ”G”, trade name “Pro Leather FG-F” (all manufactured by Amano Enzyme, Inc.) ), A peptidase derived from Rhizopus fungi (for example, trade name “peptidase R” (manufactured by Amano Enzyme Inc.)) and the like are preferably used in combination. What is necessary is just to adjust suitably the usage-amount of protein hydrolase, reaction temperature, reaction time, etc. according to the enzyme to be used.

  In this invention, the organic acid used as another active ingredient should just be a thing usable for food-drinks, Specifically, a citric acid, malic acid, an acetic acid etc. can be illustrated. In the present invention, citric acid is particularly preferably used.

  The blending ratio of the egg white hydrolyzate and the organic acid in the composition of the present invention is 0.2 to 1.2 parts by mass of the organic acid with respect to 1 part by mass of the egg white hydrolyzate (solid content), preferably the egg white hydrolyzate ( The solid content is 0.4 to 1 part by mass of organic acid with respect to 1 part by mass.

  The composition of the present invention can be used as a supplement as it is, but can be blended in various foods and drinks including sports drinks, jelly drinks, and nutrition / energy bars.

  By taking 500 mg to 10 g, preferably 1 g to 5 g of the composition of the present invention before exercise, an effect of improving cardiopulmonary function can be expected. Therefore, it is preferable to set the addition amount of the present composition in the food and drink so that the above amount can be ingested per serving.

  In addition to the above basic components, the composition of the present invention includes food additives such as excipients, emulsifiers, stabilizers, sweeteners, pH adjusters, thickeners, fragrances, acidulants, vitamins, minerals And physiologically active components such as branched chain amino acids (valine, leucine, isoleucine), anserine, carnosine, carnitine, creatine, and γ-aminobutyric acid.

  Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not particularly limited thereto. Unless otherwise specified, “%” represents “% by mass”.

  After 12 kg of liquid egg white (solid content 12.5%) was subjected to a desugaring treatment (yeast fermentation method) according to a conventional method, 3.7 L of water was added and stirred uniformly. The temperature of the egg white solution was adjusted to 60 ° C., and the enzyme reaction was performed in the following order.

  1) 30 g of papain (trade name “Papain 300”, manufactured by Nippon Biocon Co., Ltd.) was added and reacted for 1.5 hours.

  2) Adjust pH with sodium hydroxide (pH 8.0), add 5g of trypsin, and react at 55 ° C for 1 hour.

  3) Bacillus genus-derived protease (trade name “Protease S“ Amano ”G”, Amano Enzyme Co., Ltd.) 15 g was added and reacted at 65 ° C. for 1 hour.

  4) 10 g of protease derived from Bacillus genus (trade name “Protease N“ Amano ”G, manufactured by Amano Enzyme Co., Ltd.) was added and reacted at 55 ° C. for 1 hour.

  5) Add 5 g of Rhizopus fungi-derived peptidase (trade name “Peptidase R”, manufactured by Amano Enzyme) and react at 40 ° C. for 4 hours.

  After completion of the enzyme reaction, the mixture was heated at 90 ° C. for 10 minutes, filtered, and the obtained filtrate was spray-dried to obtain 900 g of egg white hydrolyzate (sample 1).

  The obtained egg white hydrolyzate was analyzed for molecular weight by high performance liquid chromatography under the following conditions. The result is shown in FIG.

Column: “YMC-Pack Dio1-60 (8.0 × 500mm)” (trade name, manufactured by YMC Corporation)
Eluent: 0.1M potassium phosphate buffer (pH 7.0)
Flow rate: 0.7mL / min Detection wavelength: 215nm

  Then, the above egg white hydrolyzate is dissolved in a suitable amount of water, and 1 part by weight of citric acid (anhydrous) is added to 1 part by weight of the egg white hydrolyzate (solid content), and then uniformly lyophilized. Thus, the composition for improving cardiopulmonary function of the present invention (Sample 2) was obtained.

Similarly, 0.4 part by mass of citric acid (anhydrous) was added to 1 part by mass of the egg white hydrolyzate (solid content) to prepare the composition for improving cardiopulmonary function (Sample 3) of the present invention.
(Test example)

An exercise load test was performed using the sample obtained above.
i) Grouping and subjects Control group: 100 mL water
Comparative group: Egg white hydrolyzate (Sample 1) 0.5% aqueous solution 100mL
Test group: Composition for improving cardiopulmonary function (Sample 2) 1% aqueous solution 100mL

  Each group consisted of 6 people (3 males and 3 females (mean age 26.3 ± 1.8 years)), and each group was tested at 1 week intervals.

ii) Test method An exercise load test was conducted with a exercise intensity of 60% VO2max using a bicycle exercise apparatus according to the protocol shown in FIG. Then, during the exercise test, the subject's heart rate was monitored, and the time from the end of the exercise test until the heart rate reached 100 beats / minute was measured to obtain the heart rate recovery time. The result of comparing the heart rate recovery time in each group is shown in FIG.

  FIG. 3 shows that the heart rate recovery time after the exercise stress test is shortened in the comparison group (sample 1) and the test group (sample 2) compared to the control group. In particular, it can be seen that the test group (sample 2) is shortened to about 2/3 compared to the control group. In addition, even when Sample 3 was used, the heart rate recovery time was reduced as much as in the above test group (Sample 2).

  From the above results, by ingesting the cardiopulmonary function improving composition of the present invention, the cardiopulmonary function can be further enhanced, and as a result, improvement in exercise performance can be expected.

  The composition for improving the cardiopulmonary function of the present invention can be suitably used not only for athletes but also for people who start exercising with metabolic measures.

It is the figure which showed the result of the gel filtration chromatography of the obtained egg white hydrolyzate. It is the figure which showed the protocol of the human test using a bicycle exercise apparatus. It is the figure which showed the result of having measured the heart rate recovery time after the exercise load test.

Claims (4)

  A composition for improving cardiopulmonary function, comprising an egg white hydrolyzate and an organic acid as active ingredients.   The composition for improving cardiopulmonary function according to claim 1, comprising 0.2 to 1.2 parts by mass of an organic acid per 1 part by mass of the egg white hydrolyzate (solid content).   The composition for improving cardiopulmonary function according to claim 1 or 2, wherein the organic acid is citric acid.   Food-drinks containing the composition for cardiopulmonary function improvement as described in any one of Claims 1-3.

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