JP2009269895A - Drug-induced liver disorder inhibitory composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition having a drug-induced liver disorder inhibitory action, and as a result, to provide foods, beverages, medicines or feeds containing the composition. <P>SOLUTION: This drug-induced liver disorder inhibitory composition is characterized by containing the components of seeds of a balsam pear (Momordica charantia), and the foods, beverages, medicines or feeds containing the same composition are also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

The present invention relates to a drug-induced liver injury-suppressing composition comprising a bitter gourd seed component.
The drug-induced liver injury-suppressing composition in the present invention refers to prevention of drug-induced liver injury, prevention of progression and progression of drug-induced liver injury, and / or improvement of liver function in patients with drug-induced liver injury, It means compositions for pharmaceuticals (including animal therapeutics), foods and drinks, and feeds given to humans or non-human animals in order to maintain normal liver function.

Drug-induced liver injury is a side effect that develops in the course of continuing drug therapy. In recent years when the frequency of drug use and the types of drugs have increased, this disease has increased. Drug-induced liver injury can be divided into two developmental mechanisms, allergic and toxic. As liver function improving agents, drugs with liver protective action, biliary action and antioxidant action are considered, and ursodeoxycholic acid, aminoethyl sulfonic acid, etc. are used, but in terms of clinical efficacy and side effects However, there is still no drug that can be satisfactorily satisfied, and a liver function improving agent that is highly effective and has few side effects is desired. In addition, turmeric, silymarin and the like are used as foods for improving liver function.

Bitter gourd (Scientific name Momorica charantia L.) is a cucurbitaceae plant also known as bitter gourd and is native to tropical Asia centering on India and is cultivated in Japan in Okinawa and southern Kyushu. The bitter gourd normal edible part (fruit) has cytotoxicity protection / restoration effect, alcohol dehydrogenase promotion effect, carcinogenesis prevention effect, blood sugar level lowering effect, lipid metabolism improving effect, hair growth effect, whitening effect, deodorizing effect It is said. However, there is no report that bitter gourd seed is effective in improving liver function.

The problem to be solved by the present invention is to provide a composition having an effect of improving liver function, and to provide a food, drink, medicine or feed containing the composition.

As a result of repeated studies to solve the above-mentioned problems, it was found that bitter gourd seeds suppress drug-induced liver injury in a carbon tetrachloride-induced drug-induced liver injury model mouse. That is, the present invention has the following configuration.
(1) A drug-induced liver injury-suppressing composition comprising a bitter gourd seed component (2) The drug-induced liver injury-suppressing composition according to (1), wherein the component is a methanol extract of bitter gourd seed (3) Food / beverage products or pharmaceuticals or feeds containing the drug-induced liver injury-suppressing composition according to (1) or (2)

According to the present invention, it is possible to provide a drug-induced liver injury-suppressing composition containing a bitter gourd seed component, and a food, drink, medicine or feed containing the composition.

“Goya seed” relating to the present invention refers to (English name: Bitter melon, scientific name: Modica charantia).

The bitter gourd seed according to the present invention can be used as it is, and can also be used as a dried or pulverized powder after drying. It can also be used as an extract extracted with an organic solvent such as hot water extraction, ethanol, or ethyl acetate.

In order to produce the drug-induced hepatic disorder inhibitor according to the present invention, bitter gourd seed powder or extract produced by the above method can be used and formulated in combination with a known non-toxic pharmaceutical guarantee according to a conventional method. That's fine. The anti-osteoporosis agent according to the present invention can be administered in various dosage forms. For example, as oral administration agents, solid agents such as tablets, granules, powders, capsules, soft capsules, solutions, suspensions Liquids such as suppositories and emulsions, lyophilized preparations, and the like, and parenteral administration agents include suppositories, propellants, transdermal absorption agents, etc., in addition to injections. It can be adjusted by means. Examples of the non-toxic collateral for pharmaceutical use include glucose, lactose, sucrose, starch, mannitol, dextrin, fatty acid glyceride, polyethylene glycol, hydroxyethyl starch, ethylene glycol, polyoxyethylene sorbitan fatty acid ester, amino acid, albumin, water And physiological saline. In addition, conventional additives such as stabilizers, lubricants, wetting agents, emulsifiers, binders and the like can be appropriately added as necessary. In the anti-osteoporosis agent according to the present invention, the dosage of momotamana powder or extract is appropriately selected and determined according to the patient's age, weight, symptoms, degree of disease, administration schedule, formulation form, etc. The dose is about 0.01 to 10 g / body weight and may be administered in several divided doses a day.
A similar formulation may be used for animals. It is appropriately selected and determined according to the age, body weight, symptom, degree of disease, administration schedule, formulation form, etc. of the animal. For example, it is about 0.01 to 10 g / kg body weight per day and divided into several times a day. May be administered.

In addition, bitter gourd seeds according to the present invention are considered to be highly safe because of their rich dietary experience, and can be taken as a functional food for the purpose of suppressing drug-induced liver injury. A functional food characterized by containing components of bitter gourd seeds according to the present invention can be positioned as a food for specified health use, a nutritional functional food, or a health food. As a functional food, for example, after adding an appropriate auxiliary agent to an extract of bitter gourd seeds, a form suitable for consumption, for example, granular, granular, tablet, capsule, paste, using conventional means What was formed in etc. can be used. This functional food may be used for food as it is, and added to various foods such as ham, sausage, kamaboko, chikuwa, bread, butter, powdered milk, confectionery, etc., water, alcoholic beverages, fruit juice, milk It may be used by adding to drinks such as soft drinks. The ingredient intake of bitter gourd seeds of the present invention in the form of such food is appropriately selected and determined according to the age, weight, symptoms, intake schedule, formulation form, etc. of the subject. For example, 0.01 to 10 g / day About kg body weight.
Similarly, in animals, for example, after adding a suitable auxiliary agent to an extract of bitter gourd seeds, it is formed into a form suitable for food, granules, granules, tablets, capsules, pastes, etc. using conventional means. Can be used. Moreover, you may add and use for feed and pet food, or may add to drinking water, such as water. The ingredient intake of bitter gourd seeds of the present invention in the form of such food is appropriately selected and determined according to the age, weight, symptoms, intake schedule, formulation form, etc. of the animal. For example, 0.01 to 10 g / day About kg body weight.

Examples are given below to describe the present invention in more detail, but the present invention is not limited by these.

Examples Inhibitory effect of bitter gourd seeds on drug-induced liver injury Carbon tetrachloride is widely used as an acute liver injury model because it provides symptoms similar to those of human drug-induced liver injury. Therefore, the following experiment was conducted to examine the effect of bitter gourd seeds on drug-induced liver injury.
Seven-week-old female ddY mice were used as experimental animals. After fasting for 24 hours, 0.6% carbon tetrachloride was forcibly administered orally to induce liver damage. 1000 mg / kg body weight of extract extracted from methanol after crushing bitter gourd seeds with a mill was forcibly administered orally 1 hour and 24 hours before administration of 0.6% carbon tetrachloride. Tail blood was collected 20 hours after administration of 0.6% carbon tetrachloride, and the serum AST / ALT activity value, which is a marker of liver damage, was measured. In the control group, distilled water was administered instead of bitter gourd seed extract. When significant suppression of serum AST / ALT activity is observed in the sample combination group as compared with the control group, it is determined that there is an effect of improving liver function. Table 1 shows the serum AST activity value ± standard error (IU / L) and the serum ALT activity value ± standard error (IU / L) of each group. The test of the significant difference of each group with respect to the carbon tetrachloride administration group was performed by Student'st-test. Compared with the carbon tetrachloride administration group, there is a significant difference at a risk rate of 0.5%, which is indicated by ## in Table 1.

From these results, it was found that bitter gourd seed methanol extract is effective for drug-induced liver injury.

Industrial applicability

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a drug-induced liver injury-suppressing composition that contains bitter gourd seed components, and a food or drink, a pharmaceutical product, or a feed containing the composition.

Claims (3)

A composition for suppressing drug-induced liver injury, comprising a bitter gourd seed component. The composition for suppressing drug-induced liver injury according to claim 1, wherein the ingredient is bitter gourd seed methanol extract. A food, beverage, medicine or feed comprising the composition for suppressing drug-induced liver injury according to claim 1 or 2.