JP2009213451A - Food and drink preventing life-style disease - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a food and drink produced for preventing life-style disease. <P>SOLUTION: The food and drink for preventing life-style disease includes a plant (the scientific name: Brassica oleracea var, gemmifera DC.×Barassica oleracea var. acepha DC) obtained by breeding Brussels sprouts and kale. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention has a plant obtained by crossing brussels sprouts and kale (scientific name: Brassica oleracea var. Gemmifera DC. X Barassica oleracea var. Acepha DC, trade name: petit veil, hereinafter also simply referred to as petit veil). In particular, the present invention relates to foods and drinks for preventing lifestyle-related diseases such as weight gain, lipid accumulation, and visceral fat.

  “Petit veil (hereinafter also referred to as PV)” was first established in Japan by the applicant of the present invention by crossing brussels sprouts and kale. Petit veil has attracted attention in recent years from companies and consumers who are focusing on improving the health of the public, including multiple media because it contains abundant nutrients such as vitamins and minerals compared to other general vegetables. Yes. However, few experiments have been conducted to examine its functionality in detail.

  In particular, considering the fact that kale, the parent strain of PV, has been reported for lifestyle-related diseases such as metabolic syndrome, there are many benefits such as high nutritional value and easy-to-eat characteristics such as sweetness. It is no exaggeration to say that PV that surpasses this point has more possibilities. Demonstration of the usefulness of PV as a functional food material is important from the above viewpoint, and future demand growth is also expected. However, the food and drink using PV which paid attention to such functionality is not yet obtained.

  The present invention has been made in view of the above circumstances, and focuses on lifestyle-related diseases such as metabolic syndrome, and by clarifying the functionality of PV, food and drink for preventing lifestyle-related diseases The purpose is to provide goods.

  The food and drink according to the present invention is a food and drink for preventing lifestyle-related diseases, and the food and drink is a plant obtained by crossing brussels sprouts and kale (scientific name: Brassica oleracea var. Gemmifera DC. X Barasica oleracea). var.acepha DC).

  In the food and drink according to the present invention, the plant is an outer leaf of the plant.

  In the food and drink according to the present invention, the plant is an inner leaf of the plant.

  The food and drink according to the present invention is characterized in that the prevention of lifestyle-related diseases is suppression of weight gain.

  In the food and drink according to the present invention, the prevention of the lifestyle-related diseases is suppression of an increase in blood triglycerides.

  The food and drink according to the present invention is characterized in that the prevention of lifestyle-related diseases is suppression of an increase in visceral fat.

  The food and drink according to the present invention is characterized in that the visceral fat is a fat selected from the group consisting of mesenteric fat, perirenal fat and testicular fat.

  In the food or drink according to the present invention, the suppression of the increase in blood triglyceride is expressed by the suppression of activation of liver fatty acid synthase.

  In the food and drink according to the present invention, the suppression of the increase in visceral fat is expressed by the suppression of activation of liver fatty acid synthase.

  In the food and drink according to the present invention, the suppression of the increase in blood triglyceride is not mediated by fluctuations in the activity of liver carnitine acyltransferase.

  The food or drink according to the present invention is characterized in that the suppression of the increase in visceral fat is not mediated by fluctuations in the activity of liver carnitine acyltransferase.

  On the other hand, the food and drink according to the present invention is a food and drink for suppressing the activation of liver fatty acid synthase, and the food and drink is a plant obtained by crossing kale and Brussels sprouts (scientific name: Brassica oleracea var). Gemmifera DC. × Barassica oleracea var.acepha DC).

  According to the present invention, it is possible to prevent various lifestyle-related diseases such as an increase in body weight, an increase in blood triglycerides, and an increase (accumulation) of visceral fat.

  Further, according to the present invention, it is possible to suppress the activation of liver fatty acid synthase.

  The food and drink according to the present invention has a petit veil. The petit veil may be derived from various parts constituting the petit veil, and may be, for example, the outer leaf of the petit veil or the inner leaf of the petit veil.

  There is no restriction | limiting in particular as the form of the food-drinks by this invention, For example, flake form, powder form, granule form, fine granule, tablet, pellet, paste, emulsion, suspension, solution are mentioned.

  Examples of the food and drink according to the present invention include noodles such as rice noodles, pasta and ramen, confectionery such as cakes, strawberries and cookies, beverages such as fresh juices, concentrated juices, juices obtained by dissolving / suspending powders, and the like. Illustrated.

  Various ingredients such as excipients, diluents and / or additives known in the art may be added to the preparation of the above-mentioned form of the food and drink according to the present invention.

  In the present invention, the term “lifestyle-related diseases” is a term that collectively refers to various diseases related to lifestyle habits, including metabolic syndrome, and in particular, a series of diseases caused by a high-fat diet. A group. Therefore, the term “lifestyle-related diseases” includes an increase in body weight, an increase in visceral fat (accumulation), and an increase in blood triglyceride level.

  In the present invention, the term "plant obtained by crossing brussels sprouts and kale" refers to a plant obtained by crossing brussels sprouts and kale as described above, for example, cabbage x kale (Brassica oleracea var) Gemmifera DC. X Barassica oleracea var. Acepha DC) refers to a group of plants. The term “outer leaf” refers to a leaf directly attached to and extending from the central axis of a plant (petit veil) obtained by crossing brussels sprouts and kale, and the term “inner leaf” A side bud directly attached to the central axis of the petit veil.

  The food and drink according to the present invention is for preventing lifestyle-related diseases. Preventing this lifestyle-related disease includes suppressing weight gain, suppressing blood triglyceride increase, and suppressing visceral use (accumulation). Therefore, the food and drink according to the present invention may be a food and drink for suppressing weight gain, may be a food and drink for suppressing an increase in blood triglycerides, and / or increase in visceral fat ( It may be a food or drink for suppressing (accumulation).

  In the present invention, the visceral fat includes various visceral fats, and may be visceral fats related to so-called metabolic syndrome. In particular, mesenteric fat, perirenal fat and testicular fat are mentioned.

  As described above, the food and drink according to the present invention include food and drink for suppressing an increase in blood triglycerides, and also includes a food and drink for suppressing an increase (accumulation) of visceral fat. Such an inhibitory effect may be expressed, for example, by suppressing the activity of fatty acid synthase in the liver activated by ingestion of a high fat diet. Further, such an inhibitory effect may not be mediated by a change in the activity of liver carnitine acyltransferase. Therefore, the food and drink according to the present invention may be a food and drink for suppressing activation of liver fatty acid synthase.

[Animals and their breeding]
A 10-week-old male ICR mouse was used, and this was used as a high-fat, high-sucrose, high-cholesterol obesity-inducing diet (Western diet) having the composition shown in FIG. Each of the feeds to which Minivale No. 6) (PV) was added was bred for a predetermined period (hereinafter also referred to as W group and WPV group, respectively). As a control group, a normal feed having the composition shown in FIG. 1-2 and a feed obtained by adding PV to the normal feed at 5% were used. In each group, 5 or more animals were used.

[Measurement of weight]
The weight (g) of the test animal was measured according to a conventional method.

[Measurement of organ weight]
After the animals were sacrificed under anesthesia, each organ was surgically collected and the weight of each organ was measured according to a standard method.

[Measurement of fat weight]
After the animals were sacrificed under anesthesia, the mesentery, kidney and testis were surgically collected. The fat adhering to these organs / tissues was surgically removed, and the weight of each fat was measured according to a standard method.

[Quantification of blood cholesterol and blood triglycerides]
Venous blood was collected from the inferior vena cava of a subject animal under anesthesia and its plasma was obtained. About this plasma, blood cholesterol and blood triglyceride (mg / mL) were measured according to the cholesterol oxidase method and the glycerol-3-phosphate oxidase method.

[Quantification of liver fatty acid synthase activity and carnitine acyltransferase activity]
After sacrifice of the animal under anesthesia, the liver was surgically collected. The collected liver was subjected to FAS extraction buffer (0.1 M KH ₂ PO ₄ , 0.25 M sucrose, 1 mM EDTA, 0.05 M 2-mercaptoethanol (pH 7.0)) and CPT extraction buffer (3 mM Tris-HCl, 1 mM EDTA). , 0.25M sucrose (pH 7.2)), the resulting suspension was centrifuged to obtain the supernatant.

  For fatty acid synthase, a reaction solution comprising the following components was prepared, and the supernatant obtained as described above was subjected to liver fatty acid synthase activity (NADPH / min) by a colorimetric method using NADPH at a wavelength of 340 nm. / Mg soluble protein) was quantified.

Final composition of the reaction solution Preparative amount Potassium phosphate buffer (pH 7.0) 100 mM
EDTA 0.2 mM buffer 500 μL
NADPH 0.30 mM 15 μL
Acetyl-CoA 0.05 mM 10 μL
Malonyl-CoA 0.20 mM 10 μL
Crude enzyme solution (supernatant) 50 μL
ddH ₂ O 415 μL
1mL total volume

  For carnitine acyltransferase, a reaction solution comprising the following components was prepared, and the supernatant obtained as described above was subjected to a ratio of 412 nm using 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB). Liver carnitine acyltransferase activity (DTNB / min / mg soluble protein) was quantified by colorimetry.

Final composition of reaction liquid Preparative amount Tris-HCl (pH 8.0) 58.0 mM
EDTA 1.25mM
Triton X-100 0.10%
DTNB 0.25 mM Buffer 500 μL
Palmitoyl-CoA 0.04 mM 20 μL
L-carnitine 1.25 mM 10 μL
Crude enzyme solution (supernatant) 10 μL
ddH ₂ O 460 μL
1mL total volume

[statistics]
Statistical significance was determined by Turkey-Kramer's multiple comparison test (P <0.05, significant difference between different characters).

Example 1
As described above, mice were used as test animals, and changes in mouse weights were observed for groups W and WPV when they were raised to 30 weeks of age. The results are shown in FIG. The total feed intake during this example is shown in FIG. 2-2.

  In CT in which a normal diet was fed to 10-week-old mice, body weight increased to 55 grams until 30-week-old. In the PV section where 5% of PV was added to the normal diet, the growth was almost the same and increased to 55 grams, and no significant difference was observed between them (FIG. 2-1). On the other hand, in mice fed a high fat, high sucrose, high cholesterol obesity-inducing diet (Western diet: W), the body weight began to increase markedly from around 17 weeks of age, and finally increased significantly compared to CT. Indicated.

  On the other hand, in the mice fed with the feed obtained by adding PV to the Western diet, the significant increase in the body weight of the mice fed with the Western diet alone remained at almost the same level as the CT.

  In addition, the total intake of the feed consumed less Western feed compared to the normal feed, which was 231 kCal per 100 grams of feed compared to 450 kCal for the Western feed. It was thought that this was because the calories were nearly doubled (FIG. 2-2). However, no significant increase or decrease in intake due to the addition of PV was observed in any feed.

(Example 2)
In Example 1, the blood cholesterol level and blood triglyceride level were quantified as described above for the blood of a mouse (30 weeks old) reared 20 weeks after the start of the experiment. The results are shown in FIGS. 3 and 4, respectively.

  As a result, although the influence of PV was not recognized in the blood component at the time of giving a normal feed similarly to the weight increase observed in Example 1, when a Western feed was fed, the remarkable increase was confirmed. . Moreover, about the group reared with the Western feed which added PV, it confirmed that the increase by a Western feed was suppressed especially in the quantity of the blood triglyceride.

(Example 3)
In Example 1, organs of each group (10 weeks old) reared at 10 weeks were collected as described above, and as described above, the weight of each organ and the weight of each tissue and fat were measured as described above. . The result is shown in FIG. In addition, in this example, in addition to the WPV group, a group reared using a feed obtained by adding 5% petitvale outer leaves and inner leaves to Western feed was set, and the same treatment as the WPV group was performed. It was. About these groups, it shows as "PV (outer leaf)" and "PV (bud)", respectively in FIG. Moreover, the group which added the outer leaf and the inner leaf to the normal feed at the respective ratios was used as the target group of the group raised using the Western feed to which the outer leaf and the inner leaf were added.

  Since changes in lipid components in the blood were confirmed as shown in Example 2, it was examined what changes were actually made in the weights of animal organs and the like. Increased fat (mesenteric fat, adrenal fat, testicular fat) was observed. On the other hand, increases in liver weight, intraperitoneal fat (mesenteric fat, adrenal gland fat, testicular fat) and the like observed in the W group can be suppressed in the group fed with Western feed supplemented with PV. It was confirmed (FIG. 5). Furthermore, it was found that this result shows not only the outer leaves of PV used so far but also the inner leaves (buds).

Example 4
In Example 1, liver homogenates were prepared from mice reared at 10 weeks of age (20 weeks of age), and the activities of fatty acid synthase and carnitine acyltransferase were each quantified as described above. The results are shown in FIGS. 6 and 7, respectively.

  As in Examples 2 and 3, in order to examine the anti-obesity inhibitory action of PV observed in blood components and organ weights in more detail, fatty acid synthase (FAS) in the liver that controls lipid metabolism in the body and The activity of carnitine acyltransferase (CPT) was examined using the liver obtained in the same animal experiment as described above. In the Western feed section, FAS activity increased remarkably, but in PV, both outer leaves and inner leaves (buds) suppressed the increase due to W (FIG. 6). On the other hand, no significant difference was observed in the feed section in CPT (FIG. 7).

(Summary)
In the present invention, normal feed group (CT), normal feed + petit veil group (PV), high fat high sucrose high cholesterol obesity induction group (Western feed) (W), Western feed + Petit veil group (WPV) The following four experimental plots were set and the weight gain of the mice was compared. As a result, although the feed intake was almost the same, the PV mixed feed significantly suppressed the weight gain ( Fig. 2-1). Furthermore, in visceral fat (mesenteric fat, perirenal fat, testicular fat), which is an index of metabolic syndrome, it was confirmed that weight increase was clearly suppressed in WPV compared with W (FIG. 5). . In addition, liver weight showed a similar tendency (FIG. 5). The weight gained by the obesity-induced diet is closely related to the increase in visceral fat weight, suggesting that petitvale may be able to suppress metabolic syndrome.

  In biochemical tests of blood, petitval has also been observed to suppress increases in total lipid and triglycerides in blood caused by an obesity-induced diet. These results indicate that PV has the action of suppressing fat accumulation by controlling lipid metabolism in the living body, and is a powerful material that can present the direction of prevention or improvement of metabolic syndrome from the viewpoint of lipid metabolism. It means to become. Therefore, the activity of liver fatty acid synthase (FAS) and carnitine acyltransferase (CPT), which play an important and decisive role in lipid metabolism in the living body, was examined. (Fig. 6). On the other hand, none of the feeds showed any effect on CPT (FIG. 7). This means that FAS and CPT have an important role in fat accumulation and degradation, and that petitval suppresses FAS activity, which is particularly responsible for accumulation. Except that 5% petit veil is contained, petit veil suppressed the fat accumulation mechanism of the living body as represented by FAS, despite the same feed composition and feed intake as the W ward. It is clear that this fact strongly supports the data obtained in FIGS. Furthermore, these results are the same not only in the outer leaves of petit veil but also in the inner leaves (side buds) (FIGS. 5, 6, and 7), and it is clear that the results can be obtained as a whole vegetable called petit veil. It is.

The composition of the Western feed is shown. The composition and nutritional value of normal feed are shown. It is a graph which shows a weight change. It is a graph which shows feed total intake. A graph showing the amount of total cholesterol in the blood is shown. 2 is a graph showing the amount of total triglycerides in blood. It is a graph which shows the weight of each organ and each visceral fat. It is a graph which shows the activity of the fatty acid synthetase (FAS) of a liver. It is a graph which shows the activity of a liver carnitine acyltransferase (CPT).

Claims (14)

Food and drink to prevent lifestyle-related diseases,
The said food-drinks have the plant (scientific name: Brassica oleracea var. Gemmifera DC. X Barassica oleracea var. Acepha DC) obtained by crossing the brussels sprouts and kale.   The food or drink according to claim 1, wherein the plant is an outer leaf of the plant.   The food or drink according to claim 1, wherein the plant is an inner leaf of the plant.   The food and drink according to any one of claims 1 to 3, wherein the prevention of lifestyle-related diseases is suppression of weight gain.   The food / beverage product according to any one of claims 1 to 4, wherein the prevention of lifestyle-related diseases is suppression of an increase in blood triglycerides.   The food or drink according to any one of claims 1 to 5, wherein the prevention of lifestyle-related diseases is suppression of an increase in visceral fat.   The food and drink according to claim 6, wherein the visceral fat is a fat selected from the group consisting of mesenteric fat, perirenal fat, and peritesticular fat.   The food / beverage product according to claim 5, wherein the suppression of increase in blood triglyceride is expressed by suppression of activation of liver fatty acid synthase.   The food / beverage product according to claim 6 or 7, wherein the suppression of the increase in visceral fat is expressed by suppression of activation of fatty acid synthase in the liver.   The food / beverage product according to claim 5 or 8, wherein the suppression of the increase in blood triglyceride is not mediated by a change in the activity of liver carnitine acyltransferase.   10. The food or drink according to claim 6, 7 or 9, wherein the suppression of the increase in visceral fat is not mediated by fluctuations in the activity of liver carnitine acyltransferase. It is a food or drink for suppressing the activation of fatty acid synthase in the liver,
The said food-drinks have the plant (scientific name: Brassica oleracea var. Gemmifera DC. X Barassica oleracea var. Acepha DC) obtained by mating kale and Brussels sprouts.   The food / beverage product according to claim 12, wherein the plant is an outer leaf of the plant.   The food or drink according to claim 12, wherein the plant is an inner leaf of the plant.