JP2009198709A5 - - Google Patents

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JP2009198709A5
JP2009198709A5 JP2008039066A JP2008039066A JP2009198709A5 JP 2009198709 A5 JP2009198709 A5 JP 2009198709A5 JP 2008039066 A JP2008039066 A JP 2008039066A JP 2008039066 A JP2008039066 A JP 2008039066A JP 2009198709 A5 JP2009198709 A5 JP 2009198709A5
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image
low
resolution
magnification
imaging optical
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JP2008039066A
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JP2009198709A (en
JP5292846B2 (en
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Claims (8)

被観察物の像を撮像し、少なくとも2つの前記被観察物の分解能の異なる画像を取得する撮像手段と、
前記撮像手段によって相対的に高い分解能で得られた画像の所定領域における画像特徴量に、相対的に低い分解能で得られた画像の前記所定領域における画像特徴量略等しくなるように、前記分解能の低い画像の画像処理のための基準値を算出する制御手段と、
前記基準値に基づき前記分解能の低い画像全体に亘り画像処理する画像処理手段と
有することを特徴とする観察装置。
An imaging means for imaging an image of the observed object, it acquires a different image resolution of at least two of said object under observation,
The image characteristic amount in a predetermined area of an image obtained at a relatively high resolution by the imaging means, as the image feature amount in the predetermined region of an image obtained at a relatively low resolution is substantially equal, the resolution Control means for calculating a reference value for image processing of a low image ,
Image processing means for performing image processing over the entire low-resolution image based on the reference value ;
Observation apparatus characterized by having a.
前記撮像手段は、前記被観察物の像を撮像する倍率の異なる少なくとも2つの撮像光学系を含み、
前記制御手段は、前記分解能の高い前記撮像光学系の高倍率画像で求められる所定領域における画像特徴量が、前記分解能の低い前記撮像光学系の低倍率画像で求められる前記所定領域における画像特徴量と略等しくなるように、前記低倍率画像の画像処理のための基準値を算出することを特徴とする請求項1に記載の観察装置。
The imaging means includes at least two imaging optical systems having different magnifications for imaging an image of the object to be observed,
The control means is configured such that an image feature quantity in a predetermined area obtained from a high-magnification image of the imaging optical system with a high resolution is an image feature quantity in the predetermined area obtained from a low-magnification image of the imaging optical system with a low resolution. The observation apparatus according to claim 1 , wherein a reference value for image processing of the low-magnification image is calculated so as to be substantially equal to .
前記高倍率画像を撮像する前記撮像光学系の視野は、前記低倍率画像を撮像する前記撮像光学系の視野に含まれることを特徴とする請求項に記載の観察装置。 The observation apparatus according to claim 2 , wherein a field of view of the imaging optical system that captures the high-magnification image is included in a field of view of the imaging optical system that captures the low-magnification image . 前記高い分解能の撮像光学系の視野中心と前記低い分解能の撮像光学系の視野中心とが一致することを特徴とする請求項1から3のいずれか1項に記載の観察装置。 Observation device according to any one of claims 1 to 3, characterized in that the center of the field of view of the imaging optical system of the lower resolution and field of view center of the imaging optical system of the high resolution match. 前記画像特徴量は、前記被観察物である細胞の前記撮像された画像に占める細胞占有率、あるいは前記撮像された画像の輝度ヒストグラムからなることを特徴とする請求項1からのいずれか1項に記載の観察装置。 The said image feature amount consists of the cell occupation rate which occupies for the said imaged image of the cell which is the said to-be-observed object , or the luminance histogram of the said imaged image, The any one of Claim 1 to 4 characterized by the above-mentioned. The observation apparatus according to item. 前記基準値は、前記被観察物である細胞の前記撮像された画像に占める細胞占有率が所定値となるときの前記画像の輝度値からなることを特徴とする請求項1からのいずれか1項に記載の観察装置。 The said reference value consists of the luminance value of the said image when the cell occupation rate which occupies for the said imaged image of the cell which is the said to-be-observed object becomes a predetermined value, The any one of Claim 1 to 5 characterized by the above-mentioned. The observation apparatus according to item 1. 前記被観察物を照明し、明部と暗部とが周期的に配置された面光源と、  Illuminating the object to be observed, a surface light source in which bright parts and dark parts are periodically arranged;
前記画像処理手段で処理された前記分解能の低い画像の処理結果を表示する表示手段と、  Display means for displaying the processing result of the low resolution image processed by the image processing means;
を備えたことを特徴とする請求項1から6のいずれか1項に記載の観察装置。The observation apparatus according to any one of claims 1 to 6, further comprising:
被観察物を倍率の異なる少なくとも2つの撮像光学系で同じ領域を含んで高倍率画像と低倍率画像とを撮像し、前記同じ領域の前記高倍率画像と低倍率画像との画像特徴量が略等しくなるように前記低倍率画像の基準値を算出し、算出された前記基準値に基づき前記低倍率画像全体を処理し前記低倍率画像の処理結果を表示手段に表示する観察方法。   A high-magnification image and a low-magnification image including the same area are captured by at least two imaging optical systems having different magnifications of the object to be observed, and the image feature amounts of the high-magnification image and the low-magnification image in the same area are approximately An observation method of calculating a reference value of the low-magnification image so as to be equal, processing the entire low-magnification image based on the calculated reference value, and displaying the processing result of the low-magnification image on a display unit.
JP2008039066A 2008-02-20 2008-02-20 Observation device and observation method Active JP5292846B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008039066A JP5292846B2 (en) 2008-02-20 2008-02-20 Observation device and observation method

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Application Number Priority Date Filing Date Title
JP2008039066A JP5292846B2 (en) 2008-02-20 2008-02-20 Observation device and observation method

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JP2009198709A JP2009198709A (en) 2009-09-03
JP2009198709A5 true JP2009198709A5 (en) 2011-09-08
JP5292846B2 JP5292846B2 (en) 2013-09-18

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WO2011089908A1 (en) * 2010-01-20 2011-07-28 株式会社ニコン Cell observation device and cell culture method
WO2012029817A1 (en) 2010-08-30 2012-03-08 三洋電機株式会社 Observation device, observation program, and observation system
JP5274731B1 (en) 2011-12-22 2013-08-28 三洋電機株式会社 Observation system, program, and control method for observation system
JP5867194B2 (en) * 2012-03-13 2016-02-24 株式会社島津製作所 microscope
JP6742724B2 (en) * 2015-12-28 2020-08-19 シスメックス株式会社 Cell region determination method, cell imaging system, cell image processing device, and computer program
JP7030953B2 (en) * 2018-02-20 2022-03-07 富士フイルム株式会社 Judgment device, cell detachment recovery device and judgment method
JP7109130B2 (en) * 2018-04-04 2022-07-29 株式会社エビデント microscope

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JPH1195125A (en) * 1997-09-22 1999-04-09 Olympus Optical Co Ltd System and method for photographing microscopic digital image
JP4462959B2 (en) * 2004-02-25 2010-05-12 富士通株式会社 Microscope image photographing system and method
JP5059637B2 (en) * 2008-01-18 2012-10-24 オリンパス株式会社 Microscope imaging device

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