JP2009136982A - Tweezers - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To hold a sheet-like cultured cell on a part of a planar adhesive holding face 6a in a state of being developed in a wide area, and effectively prevent suspended other part of the sheet-like cultured cell from being overlapped on each other and adhered to each other. <P>SOLUTION: Tweezers 1 have two leg portions 2, 3 connected to each other openably and closably, so that grip portions 4, 5 provided on the tip part of each leg portion are mutually approached or separated to grip an article. An adhesive holding member 6 is provided in a leg portion 3 in such a manner as protruding to the opposite side to the side where both grip portions 4 and 5 are faced with each other, with this adhesive holding member 6 having a planar adhesive holding face 6a. By using the tweezers 1, when the sheet-like cultured cell cultured in a Petri dish is gripped and suspended, an end portion of a sheet-like cultured cell is held by both grip portions 4 and 5, and the adhesive holding face 6a can be brought into contact with the sheet-like cultured cell and adhered thereto, while raising the tweezers. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to tweezers, and more particularly to tweezers suitable for gripping sheet-like cultured cells such as cultured cells.

As is well known in the art, a tweezer has two legs connected to each other so as to be openable and closable, and grips an article by bringing a gripping portion provided at the tip of each leg into contact with and away from each other. It has become.
In general, the grip portion of the tip portion has a rectangular shape or a long and narrow triangle shape in which the tip side is slightly narrowed.
Conventionally, in order to easily grip sheet-like cultured cells and sheet-like firibs, tacocombs, navels, etc. used for hemostasis, the gripping part has a U-shaped or Y-shaped shape with a wider area. There has been proposed one that can hold the sheet-like cultured cells (Patent Document 1).
JP 2007-159823 A

By the way, for example, when holding and suspending a sheet-like cultured cell cultured in a petri dish, first, one gripping part of the tweezers is inserted between the petri dish and the sheet-like cultured cell, and in that state If the edge part of a sheet-like cultured cell is hold | gripped with both holding parts, a sheet-like cultured cell will be gradually taken out from a petri dish from the edge part, and will be suspended.
When conventionally known tweezers are used, when the end of the sheet-like cultured cell is gripped and suspended, other parts away from the end gripped by the gripping part are close to each other. If the portions overlap each other and adhere to each other, it becomes a troublesome work to develop the sheet again.
On the other hand, when the tweezers disclosed in Patent Document 1 are used, when the end of the sheet-like cultured cell is gripped and suspended, the end is gripped in a wide area. Therefore, it is possible to effectively prevent other portions that are separated from the end portion gripped by the grip portion from being overlapped and bonded. However, on the other hand, since the area of the gripping part is large, it becomes difficult to insert the gripping part between the petri dish and the sheet-like cultured cells. The larger the area of the gripping part in order to prevent it, the more prominent.
In view of the circumstances described above, the present invention provides tweezers that can easily insert a gripping portion between a petri dish and a sheet-like cultured cell and that can effectively prevent overlapping of sheet-like cultured cells. It is to provide.

That is, the present invention has two legs connected to each other so as to be openable and closable, and in a tweezers for gripping an article by bringing a gripping part provided at a tip part of each leg into and out of each other,
One leg is provided with a close contact holding member that protrudes on the opposite side of the side where the gripping portions face each other, and the close contact holding member has a flat close contact holding surface. It is.

According to the above configuration, when gripping the ends of the sheet-like cultured cells with both gripping portions, since the tightly holding member protrudes on the opposite side to the side where the both gripping portions face each other, The planar contact holding surface of the contact holding member may be brought into contact with the sheet-like cultured cell to adhere the sheet-like cultured cell to the contact holding surface.
In this state, the sheet-like cultured cells may be gradually lifted from the end of the petri dish and suspended, and in this state, adjacent portions of the ends of the sheet-like cultured cells gripped by both grips. Is held in a state of being developed in a wide area by the flat contact holding surface. Therefore, it can prevent effectively that the other suspended part of the sheet-like cultured cell overlaps and adheres.
In particular, in the present invention, the shape and area of the grip portion and the shape and area of the flat contact holding surface can be set separately. At the same time as setting the shape and area that can be easily inserted between the cultured cells, the shape and area of the flat contact holding surface is adhered to the other parts of the sheet-like cultured cells that are suspended. Can be set to a shape and area that can effectively prevent overlapping, and this makes it easy to insert the gripping part between the petri dish and the sheet-like cultured cells, and also makes the sheet-like culture. Tweezers that can effectively prevent cell overlap can be provided.

The present invention will be described below with reference to the illustrated embodiments. In FIGS. 1 to 3, tweezers 1 has two legs 2, 3 whose base ends are connected to each other so as to be openable and closable. Grip portions 4 and 5 that can be brought into contact with and separated from each other are provided at the respective tip portions of the portions 2 and 3.
The tweezers 1 are made of metal or synthetic resin, and the two gripping portions 4 and 5 are usually held in a state separated by a predetermined distance due to the elasticity of the tweezers 1 itself. When the legs 2 and 3 are pressed against each other with a finger against each other, the gripping parts 4 and 5 can be brought into contact with each other and the article can be gripped between them. .
At this time, the front ends of the legs 2 and 3 are bent in a U-shape in the direction in which the grips 4 and 5 approach each other (see FIG. 1), thereby making the legs 2 and 3 mutually When pressing in the direction approaching the two, only the grips 4 and 5 are in contact with each other.

In the illustrated embodiment, in order to efficiently grip a sheet-like cultured cell or the like (not shown), one gripping portion 4 has a shape that is wider in the lateral direction than the longitudinal direction of the leg portion 2. Yes. That is, the width of the distal end side of one leg portion 2 is narrower than that of the proximal end side, and the grip portion 4 provided at the distal end portion of the leg portion 2 is It is wider than the width and is about the width on the base end side of the leg 2.
As a result, the tip of the leg 2 has a T-shape as a whole (see FIG. 2), and the relatively wide gripping portion 4 can grip the sheet-shaped cultured cells without damaging them. At the same time, the size is set so that it can be easily inserted between the petri dish and the sheet-like cultured cells.
In addition, the holding part 4 is not limited to the T shape shown in figure, The shape and area of the said holding part 4 are set to the shape and area which are easy to insert between a petri dish and a sheet-like cultured cell. For example, it may be a rectangular shape or a long and narrow triangular shape with a slightly narrowed tip side like a general tweezers.

  The other gripping part 5 is provided with the same width as that of the leg part 3 provided with the gripping part 4. That is, the distal end side of the other leg portion 3 is about the width of the proximal end portion side, so that the grip portion 5 provided at the distal end portion of the leg portion 3 also remains the width of the distal end portion of the leg portion 3. ing. As a result, the widths of the gripping portions 4 and 5 are made substantially the same, and the sheet-shaped cultured cells are damaged by the gripping portions 4 and 5 with the same width from the front and back surfaces. It can be gripped without any problems.

On the outside of the grip portion 5, that is, on the side opposite to the side where the grip portions 4 and 5 face each other, there is provided a thin flat plate-like close-contact holding member 6 protruding to the opposite side. The holding member 6 is provided with a flat contact holding surface 6 a at an adjacent position continuous to the grip portion 5. Then, the sheet-like cultured cells grasped by the two grasping portions 4 and 5 are adhered to the planar close-contact holding surface 6a using the adhesive force of the sheet-like cultured cells themselves or the surface tension of the culture solution. Thus, the sheet-like cultured cells can be held in a stably expanded state.
In the illustrated embodiment, the contact holding surface 6a is provided so as to form an angle of approximately 90 degrees with respect to the longitudinal direction of the leg portion 3, and is formed in a ring shape. By forming the close contact holding surface 6a in a ring shape, the adhesion state of the sheet-like cultured cells to the close contact holding surface 6a can be easily confirmed through the central through hole 6b.

Further, in the illustrated embodiment, the close contact holding surface 6a can be exchanged for a different size according to the size of the sheet-like cultured cells. That is, the leg portion 3 having the close contact holding surface 6a can be detachably separated into the leg portion of the base end portion 3a and the leg portion of the distal end portion 3b. For this purpose, the engaging means 7 is provided between them. is there.
In the illustrated embodiment, the engaging means 7 is provided with a pair of engaging claws 7a on the distal end portion 3b side provided with the close contact holding surface 6a, and each engaging claw 7a is provided on the proximal end portion 3a side. By engaging with the joint portion 7b, the base end portion 3a and the tip end portion 3b can be integrally connected.
Two slits 7c are formed on both sides of the distal end portion 3b on the base end portion 3a side, and a body portion 7d having a square cross section is formed between both the slits 7c, and the outer portions of the slits 7c are respectively operated. Part 7e is provided. The engaging claws 7a are provided at the distal end portions of the respective operation portions 7e so that the respective engaging claws 7a face outward, and the respective operation portions 7e are elastically deformed by pressing the respective operation portions 7e toward the main body portion 7d. Thus, the engaging claw 7a is moved to the main body portion 7d side so that the engagement with the engaging portion 7b can be released.

A connecting protrusion 7f having a square cross section protruding toward the base end 3a is formed at the distal end of the main body 7d, and an engaging step 7g is formed between the connecting protrusion 7f and the main body 7d. It is.
On the other hand, a connecting hole 7h for receiving the connecting protrusion 7f is formed on the base end 3a side, and the engaging step 7g is fitted to the connecting hole 7h in close contact with the engaging step 7g on the end face on the base end 3a side. The base end portion 3a and the tip end portion 3b can be connected to each other at that position. At this time, the engaging claw 7a enters the proximal end portion 3a side and engages with the engaging portion 7b, thereby connecting the proximal end portion 3a and the distal end portion 3b integrally.

In the above configuration, when the sheet-shaped cultured cells are gripped by the tweezers 1, first, the back side of the sheet-shaped cultured cells that are in close contact with the petri dish or the like by the one gripping portion 4 provided on the leg 2 ( The lower edge is removed from the petri dish and inserted between them. When the grip portion 4 is inserted between the sheet-like cultured cell and the petri dish, the other grip portion 5 and the close-contact holding surface 6a are positioned away from the surface side (upper side) of the sheet-like cultured cell. It is located and does not come into contact with the sheet-like cultured cells.
When the grip 4 is inserted into an appropriate position on the back side of the sheet-like cultured cells, the legs are held with the fingers while the tweezers 1 are tilted so that the close contact holding surface 6a does not contact the surface of the sheet-like cultured cells. The sheet-like cultured cells can be grasped from the front and back surfaces by the grasping portions 4 and 5 by pressing the two and three in the direction approaching each other.
Next, when the sheet-like cultured cells are grasped by the grasping portions 4 and 5, the tweezers 1 are raised to bring the close contact holding surface 6a into contact with the surface of the sheet-like cultured cells. At this time, the close contact state between the sheet-like cultured cells and the close contact holding surface 6a can be confirmed by the through hole 6b of the close contact holding surface 6a. When the adhesion holding surface 6a is brought into contact with the surface of the sheet-like cultured cell and the sheet-like cultured cell is adsorbed to the adhesion holding surface 6a by the adhesive force of the sheet-like cultured cell, the sheet-like cultured cell is detached from the petri dish. You can do it.
In this state, since the sheet-like cultured cells are tightly held by the close-contact holding surface 6a having a relatively large area, the sheet-like cultured cells are held in an expanded state by the close-contact holding surface 6a. This makes it possible to effectively prevent the sheet-like cultured cells from overlapping and adhering.
Further, the sheet-like cultured cells cultured on the petri dish are characterized in that the surface in contact with the petri dish has higher engraftment with the affected part. The operator can easily recognize that the lower surface of the sheet-like cultured cell that is tightly held on the close contact holding surface 6a by using the tweezers of the present invention is a surface having high engraftment with the affected part.

In addition, when exchanging the contact holding surface 6a with a different size according to the size of the sheet-like cultured cells to be grasped, first, each operation portion 7e of the engaging means 7 is moved to the body portion 7d side. The engaging claws 7a are separated from the engaging portions 7b by pressing the connecting projections 7f on the distal end portion 3b side in this state and pulled out from the connecting holes 7h on the proximal end portion 3a side. The leg part of the front-end | tip part 3b and the contact | adherence holding member 6 can be isolate | separated from a leg part.
Then, when the tip portion 3b having the necessary size of the contact holding surface 6a is selected from the various sizes of the contact holding surface 6a, the connecting projection 7f of the engaging means 7 provided on the tip portion 3b is selected. While inserting into the connection hole 7h, the engagement step part 7g of the engagement means 7 is made to contact | abut to the end surface by the side of the base end part 3a. At this time, each engaging claw 7a passes over each engaging portion 7b while being expanded by each engaging portion 7b. When the engaging step 7g comes into contact with the end surface on the base end 3a side, the engaging claws 7a simultaneously engage with the engaging portions 7b by their own elasticity beyond the engaging portions 7b. Therefore, in this state, the leg portion on the distal end portion 3b side having the close contact holding surface 6a is integrally connected to the leg portion on the proximal end portion 3a side.

  Further, when transplanting the sheet-shaped cultured cells to the affected area or moving to another container, the engaged sheet 7 is placed on the affected area and then the engagement means 7 is disengaged. The leg portion of the distal end portion 3b and the close contact holding member 6 can be used separately from the leg portion of the base end portion 3a. Thereby, when transplanting a sheet-like cultured cell to an affected part or moving to another container, the tweezers 1 do not get in the way, which is convenient. Then, the operator uses the tweezers 1 on the separated base end 3a side to slowly peel off the sheet-shaped cultured cells from the legs of the distal end portion 3b and the close-contact holding member 6 while pressing the sheet-shaped cultured cells. Thus, the following operation (for example, transplantation) can be performed.

FIG. 5 shows a second embodiment of the present invention. In the above-described embodiment, the engaging means 7 is a leg portion on the tip portion 3b side where the close contact holding member 6 is provided on one leg portion 3 and the opposite side thereof. In this embodiment, the engaging means 8 is provided between the base end of one leg 2 and the base end of the other leg 3. It is provided.
The engaging means 8 of this embodiment includes an engaging groove 8a formed along the longitudinal direction of the leg 3 at the base end of one leg 3, and the leg at the base end of the other leg 2. And an engagement protrusion (not shown) formed along the longitudinal direction of the portion 2. The engagement groove 8 a is formed in, for example, a trapezoidal cross section or a T shape, and one end thereof opens to the base end side of the leg 3.
The other engagement projection (not shown) has a cross section corresponding to the cross-sectional shape of the engagement groove 8a. By engaging the engagement protrusion with the engagement groove 8a from the opening side, both leg portions 2 are formed. 3 can be integrally and detachably connected.
Further, when the engaging protrusion and the engaging groove 8a are engaged at the regular connecting position, a hemisphere is formed at the base end portion of one leg portion 3 so that both the leg portions 2 and 3 are integrally connected and held. Two engaging convex portions 8b are provided, and two hemispherical engaging concave portions (not shown) are provided at the base end portion of the other leg portion 2.
The engaging protrusions 8b and the engaging recesses are engaged with each other when the engaging protrusion and the engaging groove 8a are engaged with each other at the normal connection position, whereby the two leg portions 2, 3 is stably connected and held together. On the other hand, when separating the two leg portions 2 and 3, if the engagement protrusion is separated from the engagement groove 8a while forcibly releasing the engagement between the engagement projection 8b and the engagement recess. Good.
Also in the present embodiment, as in the above embodiment, the leg portion 3 provided with the close contact holding member 6 is provided with a plurality of types in which the close contact holding member 6 is different in size, that is, the close contact holding surface 6a is different in size. One leg portion is selected from the plurality of types of leg portions 3 and is detachably attached to the other leg portion 2 via the engaging means 8.

In the above-described embodiment, the close contact holding surface 6a forms an angle of approximately 90 degrees with respect to the longitudinal direction of the leg portion 3, but is not limited to an angle of approximately 90 degrees. For example, it can be in the range of 30 to 150 degrees with respect to the longitudinal direction.
Further, the shape of the close contact holding surface 6a is not limited to the ring shape. For example, it may be square, trapezoidal, or fan-shaped.
Further, the contact holding surface 6a is planar, but it may not be completely planar. For example, it may be a curved surface that is curved toward the proximal end portion 3a side as it is farther from the grip portion 5, and the flat shape of the close contact holding surface 6a is a concept including such a curved surface.
Moreover, you may manufacture the said contact | adherence holding | maintenance part 6 with a transparent or translucent material.
Furthermore, the sheet-like cultured cells include not only cultured skin, cultured epithelium and cultured dermis, but also corneal cells, retinal cells, periosteal cells and other adherent cells cultured in a sheet shape, for example.

The front view which shows 1st Example of this invention. The perspective view of FIG. The left view of FIG. The perspective view which shows the leg part of the front-end | tip part 3b in one leg part 3. FIG. The disassembled perspective view in 2nd Example of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Tweezers 2, 3 Leg part 3a Base end part 3b Tip part 4, 5 Grasping part 6 Contact | adherence holding member 6a Contact | adherence holding surface 6b Through-hole 7, 8 Engagement means

Claims (7)

In tweezers having two legs connected to each other so as to be openable and closable, and gripping an article by bringing a gripping part provided at a tip part of each leg into contact with each other,
A tweezers characterized in that one leg is provided with a close-contact holding member that protrudes on the opposite side of the side where both gripping portions face each other, and the close-contact holding member has a flat close-contact holding surface. .   The tweezers according to claim 1, wherein the contact holding surface is provided at an angle of approximately 90 degrees with respect to the longitudinal direction of the leg portion.   The tweezers according to claim 1 or 2, wherein the contact holding surface is formed in a ring shape having a through hole in a central portion.   The contact holding member provided on the one leg is separable from the other leg, and the contact holding member is detachable from the other leg via an engaging means. The tweezers according to any one of claims 1 to 3, wherein the tweezers are attached.   The engaging means is provided between a leg portion on the distal end side where the close contact holding member in the one leg portion is provided and a leg portion on the base end side on the opposite side, and the close contact holding member 5. The tweezers according to claim 4, wherein the leg portion on the distal end side provided with the detachable attachment is detachably attached to the leg portion on the base end portion side through the engaging means.   The engaging means is provided between a base end portion of the one leg portion and a base end portion of the other leg portion, and a base end portion of the one leg portion provided with the close-contact holding member is The tweezers according to claim 4, wherein the tweezers are detachably attached to the base end portion of the other leg portion via the engaging means.   The leg portion provided with the close-contact holding member is provided with a plurality of types having different sizes of the close-contact holding member, and one leg portion is selected from the plurality of types of leg portions, and the other leg portion is selected. The tweezers according to claim 5 or 6, wherein the tweezers are detachably attached to the legs.

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