JP2009109933A5

JP2009109933A5 -

Info

Publication number: JP2009109933A5
Application number: JP2007284758A
Authority: JP
Filing date: 2007-11-01
Publication date: 2010-12-16

Claims

A laser light source unit that emits at least two types of excitation light having different wavelengths;
A condensing unit for condensing the two types of excitation light onto the sample;
Is disposed between the condensing section and the laser light source unit, the two types of excitation light causes incident on the condensing unit, two kinds of fluorescence generated in the specimen in accordance with two kinds of excitation light a dialog black dichroic mirror comprising a dielectric multilayered film that leads to the detection section,
In a laser-excited fluorescence microscope equipped with
Wavelength characteristic curve of the dichroic mirror,
The first excitation light band covering a wavelength band of the excitation light of one of the two types of excitation light,
A first fluorescence band covering a wavelength band of fluorescence generated according to the one excitation light;
A second excitation light band covering a wavelength band of the other excitation light of the two kinds of excitation light,
A second fluorescence band covering a wavelength band of fluorescence generated according to the other excitation light,
The reflectance or transmittance of each of the first excitation light band , the second excitation light band , the first fluorescence band, and the second fluorescence band is 95% or more ,
Each of the wavelength width of the first fluorescence band and the wavelength width of the second fluorescence band is 25 nm or more,
The first excitation light band and the first variation width between the fluorescent band, and that each variation between the second excitation light band and the second fluorescent zone is 6nm or less A laser-excited fluorescence microscope.

Each of the first excitation light band and the second excitation light band is a reflection band;
The laser-excited fluorescence microscope according to claim 1, wherein the first fluorescence band and the second fluorescence band are transmission bands.

In the laser excitation fluorescence microscope of Claim 2 ,
The transmittance of each of the first fluorescence band and the second fluorescence band is:
A laser-excited fluorescence microscope characterized by a value of 98% or more over 90% or more of the wavelength width.

In the laser excitation fluorescence microscope as described in any one of Claims 1-3 ,
The gap between the first fluorescence band and the second fluorescence band is:
A laser-excited fluorescence microscope characterized by being 20 nm or less.

In the laser excitation fluorescence microscope as described in any one of Claims 1-4 ,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by satisfying an equation of 0 ° <θ <45 °.

In the laser excitation fluorescence microscope as described in any one of Claims 1-4 ,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by satisfying an equation of 10 ° <θ <25 °.

The laser excitation fluorescent microscope according to any one of claims 1 to 4,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by satisfying an equation of 10 ° <θ <15 °.

In the laser excitation fluorescence microscope as described in any one of Claims 1-4 ,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by being 12 °.

In the laser excitation fluorescence microscope as described in any one of Claims 1-8 ,
The detector is
Laser excitation fluorescent microscope and detects the spectrum of the light transmitted through the dichroic mirror.