JP2009109933A5 - - Google Patents
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- JP2009109933A5 JP2009109933A5 JP2007284758A JP2007284758A JP2009109933A5 JP 2009109933 A5 JP2009109933 A5 JP 2009109933A5 JP 2007284758 A JP2007284758 A JP 2007284758A JP 2007284758 A JP2007284758 A JP 2007284758A JP 2009109933 A5 JP2009109933 A5 JP 2009109933A5
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- fluorescence
- band
- excitation light
- laser
- excitation
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Claims (9)
前記2種類の励起光を標本へ集光する集光部と、
前記レーザ光源部と前記集光部との間に配置され、前記2種類の励起光を前記集光部へ入射させると共に、それら2種類の励起光に応じて前記標本で発生した2種類の蛍光を検出部へ導く誘電体多層膜からなるダイクロイックミラーと、
を備えたレーザ励起蛍光顕微鏡において、
前記ダイクロイックミラーの波長特性カーブは、
前記2種類の励起光のうち一方の励起光の波長帯域をカバーする第1の励起光帯域と、
前記一方の励起光に応じて発生する蛍光の波長帯域をカバーする第1の蛍光帯域と、
前記2種類の励起光のうち他方の励起光の波長帯域をカバーする第2の励起光帯域と、
前記他方の励起光に応じて発生する蛍光の波長帯域をカバーする第2の蛍光帯域とを有し、
前記第1の励起光帯域、前記第2の励起光帯域、前記第1の蛍光帯域、及び前記第2の蛍光帯域の各々の反射率又は透過率は95%以上であり、
前記第1の蛍光帯域の波長幅及び前記第2の蛍光帯域の波長幅の各々は25nm以上であり、
前記第1の励起光帯域と前記第1の蛍光帯域との間の変化幅、及び前記第2の励起光帯域と前記第2の蛍光帯域との間の変化幅の各々は6nm以下である
ことを特徴とするレーザ励起蛍光顕微鏡。 A laser light source unit that emits at least two types of excitation light having different wavelengths;
A condensing unit for condensing the two types of excitation light onto the sample;
Is disposed between the condensing section and the laser light source unit, the two types of excitation light causes incident on the condensing unit, two kinds of fluorescence generated in the specimen in accordance with two kinds of excitation light a dialog black dichroic mirror comprising a dielectric multilayered film that leads to the detection section,
In a laser-excited fluorescence microscope equipped with
Wavelength characteristic curve of the dichroic mirror,
The first excitation light band covering a wavelength band of the excitation light of one of the two types of excitation light,
A first fluorescence band covering a wavelength band of fluorescence generated according to the one excitation light;
A second excitation light band covering a wavelength band of the other excitation light of the two kinds of excitation light,
A second fluorescence band covering a wavelength band of fluorescence generated according to the other excitation light,
The reflectance or transmittance of each of the first excitation light band , the second excitation light band , the first fluorescence band, and the second fluorescence band is 95% or more ,
Each of the wavelength width of the first fluorescence band and the wavelength width of the second fluorescence band is 25 nm or more,
The first excitation light band and the first variation width between the fluorescent band, and that each variation between the second excitation light band and the second fluorescent zone is 6nm or less A laser-excited fluorescence microscope.
前記第1の蛍光帯域及び前記第2の蛍光帯域は、透過帯域であることを特徴とする請求項1記載のレーザ励起蛍光顕微鏡。 The laser-excited fluorescence microscope according to claim 1, wherein the first fluorescence band and the second fluorescence band are transmission bands.
前記第1の蛍光帯域及び前記第2の蛍光帯域の各々の透過率は、
波長幅の90%以上に亘り98%以上の値を示す
ことを特徴とするレーザ励起蛍光顕微鏡。 In the laser excitation fluorescence microscope of Claim 2 ,
The transmittance of each of the first fluorescence band and the second fluorescence band is:
A laser-excited fluorescence microscope characterized by a value of 98% or more over 90% or more of the wavelength width.
前記第1の蛍光帯域と前記第2の蛍光帯域との間隙は、
20nm以下である
ことを特徴とするレーザ励起蛍光顕微鏡。 In the laser excitation fluorescence microscope as described in any one of Claims 1-3 ,
The gap between the first fluorescence band and the second fluorescence band is:
A laser-excited fluorescence microscope characterized by being 20 nm or less.
前記ダイクロイックミラーに対する前記励起光及び前記蛍光の入射角度θは、
0°<θ<45°の式を満たす
ことを特徴とするレーザ励起蛍光顕微鏡。 In the laser excitation fluorescence microscope as described in any one of Claims 1-4 ,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by satisfying an equation of 0 ° <θ <45 °.
前記ダイクロイックミラーに対する前記励起光及び前記蛍光の入射角度θは、
10°<θ<25°の式を満たす
ことを特徴とするレーザ励起蛍光顕微鏡。 In the laser excitation fluorescence microscope as described in any one of Claims 1-4 ,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by satisfying an equation of 10 ° <θ <25 °.
前記ダイクロイックミラーに対する前記励起光及び前記蛍光の入射角度θは、
10°<θ<15°の式を満たす
ことを特徴とするレーザ励起蛍光顕微鏡。 The laser excitation fluorescent microscope according to any one of claims 1 to 4,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by satisfying an equation of 10 ° <θ <15 °.
前記ダイクロイックミラーに対する前記励起光及び前記蛍光の入射角度θは、
12°である
ことを特徴とするレーザ励起蛍光顕微鏡。 In the laser excitation fluorescence microscope as described in any one of Claims 1-4 ,
The dichroic incident angle θ of the excitation light and the fluorescence to dichroic mirror,
A laser-excited fluorescence microscope characterized by being 12 °.
前記検出部は、
前記ダイクロイックミラーを透過した光のスペクトルを検出する
ことを特徴とするレーザ励起蛍光顕微鏡。 In the laser excitation fluorescence microscope as described in any one of Claims 1-8 ,
The detector is
Laser excitation fluorescent microscope and detects the spectrum of the light transmitted through the dichroic mirror.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007284758A JP2009109933A (en) | 2007-11-01 | 2007-11-01 | Laser-exciting fluorescence microscope |
KR1020107009514A KR20100087141A (en) | 2007-10-31 | 2008-10-29 | Laser-exciting fluorescence microscope |
AU2008320236A AU2008320236A1 (en) | 2007-10-31 | 2008-10-29 | Laser-exciting fluorescence microscope |
PCT/JP2008/003094 WO2009057301A1 (en) | 2007-10-31 | 2008-10-29 | Laser-exciting fluorescence microscope |
EP08844871.7A EP2204685B1 (en) | 2007-10-31 | 2008-10-29 | Laser-exciting fluorescence microscope |
CN200880112675.8A CN101836152B (en) | 2007-10-31 | 2008-10-29 | Laser-exciting fluorescence microscope |
US12/748,031 US8310754B2 (en) | 2007-10-31 | 2010-03-26 | Laser excitation fluorescent microscope |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007284758A JP2009109933A (en) | 2007-11-01 | 2007-11-01 | Laser-exciting fluorescence microscope |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2009109933A JP2009109933A (en) | 2009-05-21 |
JP2009109933A5 true JP2009109933A5 (en) | 2010-12-16 |
Family
ID=40778444
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007284758A Pending JP2009109933A (en) | 2007-10-31 | 2007-11-01 | Laser-exciting fluorescence microscope |
Country Status (1)
Country | Link |
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JP (1) | JP2009109933A (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5841315B2 (en) | 2010-04-28 | 2016-01-13 | ソニー株式会社 | Fine particle analyzer |
JP2017219400A (en) * | 2016-06-07 | 2017-12-14 | オリンパス株式会社 | Laser microscope |
JP6489167B2 (en) * | 2017-07-06 | 2019-03-27 | ソニー株式会社 | Data display method and fine particle analyzer |
JP2019066706A (en) | 2017-10-02 | 2019-04-25 | ソニー株式会社 | Fluorescence microscope device and fluorescence microscope system |
EP3835845A4 (en) | 2018-08-09 | 2021-09-08 | Sony Group Corporation | Optical microscope device and optical microscope system |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2798434B2 (en) * | 1989-08-31 | 1998-09-17 | 旭硝子株式会社 | Projection type color liquid crystal display |
JPH05249408A (en) * | 1992-03-06 | 1993-09-28 | Seiko Epson Corp | Projection type liquid crystal display device |
JP2004177495A (en) * | 2002-11-25 | 2004-06-24 | Olympus Corp | Microscope |
JP4493302B2 (en) * | 2003-08-06 | 2010-06-30 | オリンパス株式会社 | Scanning optical microscope |
JP2005140981A (en) * | 2003-11-06 | 2005-06-02 | Nikon Corp | Microscope |
JP4720146B2 (en) * | 2004-10-28 | 2011-07-13 | 株式会社ニコン | Spectroscopic apparatus and spectral system |
JP2006153692A (en) * | 2004-11-30 | 2006-06-15 | Nikon Corp | Spectrum detector, and microscope system |
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2007
- 2007-11-01 JP JP2007284758A patent/JP2009109933A/en active Pending
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