JP2009100702A5 - - Google Patents
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- JP2009100702A5 JP2009100702A5 JP2007277285A JP2007277285A JP2009100702A5 JP 2009100702 A5 JP2009100702 A5 JP 2009100702A5 JP 2007277285 A JP2007277285 A JP 2007277285A JP 2007277285 A JP2007277285 A JP 2007277285A JP 2009100702 A5 JP2009100702 A5 JP 2009100702A5
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- JP
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- Prior art keywords
- cells
- hepatoblasts
- medium
- embryonic stem
- stem cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Claims (4)
（ａ）胚性幹細胞から胚様体を形成する工程、及び
（ｂ）該胚様体を下記の培地：
・該培地は、肝細胞の成育に必須である１以上の物質を実質的に含まない
・該物質は、肝細胞の細胞に存在しかつ該細胞群に含まれる肝細胞の細胞以外の細胞には存在しない代謝酵素の産物である
・該培地は、該代謝酵素が該産物を生成するための基質を含む
で胚様体形成開始4日以後に培養する工程を含む方法。 A method of inducing differentiation of hepatoblasts from embryonic stem cells and obtaining a group of hepatoblasts substantially from a group of cells comprising hepatoblasts differentiated from embryonic stem cells,
(A) a step of forming an embryoid body from embryonic stem cells, and (b) the embryoid body in the following medium:
The medium is substantially free of one or more substances essential for hepatocyte growth. The substance is present in hepatocyte cells and is present in cells other than hepatocyte cells contained in the cell group. Is a product of a non-existing metabolic enzyme. The method wherein the medium comprises a step of culturing 4 days after the start of embryoid body formation, wherein the metabolic enzyme contains a substrate for producing the product.
（ａ）胚性幹細胞から胚様体を形成する工程、及び
（ｂ）該胚様体を下記の培地：
・該培地は、肝細胞の成育に必須であるＬ−アルギニン、Ｌ−チロシン、及びピルビン酸からなる群から選ばれる１以上の物質を実質的に含まない
・該培地は、さらにＤ−グルコースを実質的に含まず、Ｌ−プロリンを含む
・該培地は、肝細胞の細胞に存在する代謝酵素が代謝産物を生成するための、上記以外の基質を含む
で胚様体形成開始4日以後に培養する工程を含む方法。 A method of inducing differentiation of hepatoblasts from embryonic stem cells and obtaining a group of hepatoblasts substantially from a group of cells comprising hepatoblasts differentiated from embryonic stem cells,
(A) a step of forming an embryoid body from embryonic stem cells, and (b) the embryoid body in the following medium:
The medium is substantially free of one or more substances selected from the group consisting of L-arginine, L-tyrosine, and pyruvate that are essential for hepatocyte growth. The medium further contains D-glucose. Substantially free, including L-proline
-The medium comprises a step of culturing at least 4 days after the start of embryoid body formation , containing a substrate other than the above, so that a metabolic enzyme present in hepatocyte cells produces a metabolite .
（ａ）胚性幹細胞から胚様体を形成する工程、及び
（ｂ）該胚様体を下記の培地：
・該培地は、肝細胞の成育に必須であるＬ−アルギニン、Ｌ−チロシン、及びピルビン酸を実質的に含まない
・該培地は、さらにＤ−グルコースおよびＬ−シスチンを実質的に含まず、Ｌ−プロリンを含む
・該培地は、肝細胞の細胞に存在する代謝酵素が代謝産物を生成するための、上記以外の基質を含む
で胚様体形成開始4日以後に培養する工程を含む方法。 A method of inducing differentiation of hepatoblasts from embryonic stem cells and obtaining a group of hepatoblasts substantially from a group of cells comprising hepatoblasts differentiated from embryonic stem cells,
(A) a step of forming an embryoid body from embryonic stem cells, and (b) the embryoid body in the following medium:
The medium is substantially free of L-arginine, L-tyrosine, and pyruvate essential for hepatocyte growth. The medium is further free of D-glucose and L-cystine. Contains L-proline
· The medium, the method comprising for metabolic enzymes present in cells of the liver cells to produce a metabolite, a step of culturing the embryoid bodies formed after 4 days after in <br/> comprising a substrate other than the above.
A cell culture substantially consisting of hepatoblasts obtained by the method according to any one of claims 1 to 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007277285A JP5257971B2 (en) | 2007-10-25 | 2007-10-25 | Method for obtaining hepatoblasts from embryonic stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007277285A JP5257971B2 (en) | 2007-10-25 | 2007-10-25 | Method for obtaining hepatoblasts from embryonic stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009100702A JP2009100702A (en) | 2009-05-14 |
| JP2009100702A5 true JP2009100702A5 (en) | 2010-12-16 |
| JP5257971B2 JP5257971B2 (en) | 2013-08-07 |
Family
ID=40703183
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007277285A Expired - Fee Related JP5257971B2 (en) | 2007-10-25 | 2007-10-25 | Method for obtaining hepatoblasts from embryonic stem cells |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5257971B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012056997A1 (en) * | 2010-10-28 | 2012-05-03 | 国立大学法人熊本大学 | Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency |
| WO2013183571A1 (en) * | 2012-06-08 | 2013-12-12 | 公立大学法人名古屋市立大学 | Method for inducing differentiation of artificial pluripotent stem cell into hepatocyte |
| JP6351018B2 (en) * | 2014-02-21 | 2018-07-04 | 国立大学法人 熊本大学 | Stem cell differentiation induction method using amino acid composition modified medium, and medium kit containing the medium |
| US10006005B2 (en) * | 2014-06-27 | 2018-06-26 | National University Corporation Chiba University | Culture medium and method for inducing differentiation of pluripotent stem cells to hepatoblasts |
| WO2016027850A1 (en) | 2014-08-21 | 2016-02-25 | 味の素株式会社 | Culture medium for mesenchymal stem cells |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4759723B2 (en) * | 2004-03-12 | 2011-08-31 | 国立大学法人 千葉大学 | Method for obtaining target cells from embryonic stem cells |
-
2007
- 2007-10-25 JP JP2007277285A patent/JP5257971B2/en not_active Expired - Fee Related
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