JP2009040760A - Sterilizing and virus-removing agent and sterilizing and virus-removing method - Google Patents
Sterilizing and virus-removing agent and sterilizing and virus-removing method Download PDFInfo
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本発明は、除菌、除ウイルス剤及び除菌、除ウイルス方法に関する。詳しくは、食品工場、病院、養老施設、畜舎等の施設の床面、壁面、各種の機器等の物品に用いる除菌、除ウイルス剤及び方法に関する。 The present invention relates to a sterilization and virus removal agent and a bacteria removal and virus removal method. More specifically, the present invention relates to a sterilization and virus removal agent and method used for articles such as floor surfaces, wall surfaces, and various devices of facilities such as food factories, hospitals, nursing facilities, and barns.
食品工場、病院、養老施設、畜舎等の環境殺菌剤、医療機器、食品加工機器、調理器具等の殺菌剤として、これまで種々の殺菌剤が開発されており、アルコール系、アルデヒド系、塩素系やカチオン系の殺菌剤を例示することができる。しかし、これらの殺菌剤にはそれぞれ問題がある。例えば、アルコール系殺菌剤は、芽胞に効果がなく、揮発し易いため残効性に乏しい。アルデヒド系殺菌剤は、毒性が高く、芽胞、黴類に対する効果が弱い等の問題がある。更に、塩素系殺菌剤は、結核菌、芽胞に対する効果が弱く、有機物の存在下で速やかに分解し効力を失うという欠点がある。 Various disinfectants have been developed so far, such as environmental disinfectants for food factories, hospitals, nursing facilities, barns, medical devices, food processing equipment, cooking utensils, etc., alcohol-based, aldehyde-based, chlorine-based And cationic fungicides. However, each of these fungicides has problems. For example, alcohol-based fungicides have no effect on spores and are apt to volatilize, resulting in poor residual effect. Aldehydic fungicides have problems such as high toxicity and weak effects on spores and mosses. Furthermore, the chlorine-based disinfectant has a drawback that it has a weak effect on M. tuberculosis and spores, and it quickly degrades in the presence of organic matter and loses its efficacy.
各種施設の床面、壁面等は、細菌により汚染されており、食中毒の原因として除去、駆除すべき細菌として黄色ブドウ球菌（Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ｓｐｐ．）やセレウス菌（Ｂａｃｉｌｌｕｓ ｃｅｒｅｕｓ）などが知られているが、近年ではウイルス性の感染症が大きな社会問題となっていることもあり、ウイルスに対しても効果的な薬剤が望まれる。しかし、ノロウイルスやポリオウイルスのような一部のウイルスは、薬剤に対して強い耐性を示すため有効な薬剤が少なく、緊急時には高濃度の塩素系殺菌剤を用いて不活化されているが、日常の環境衛生において塩素系殺菌剤を用いることは臭気、腐食の点から好ましくないため、作業性に優れた除菌、除ウイルス剤が望まれている。 The floors and walls of various facilities are contaminated with bacteria, and Staphylococcus spp. And Bacillus cereus are known as bacteria to be removed and removed as the cause of food poisoning. In recent years, viral infectious diseases have become a major social problem, and effective drugs against viruses are desired. However, some viruses, such as Norovirus and Poliovirus, have few effective drugs because of their strong resistance to drugs, and are inactivated with high concentrations of chlorinated fungicides in emergency situations. Since it is not preferable to use a chlorine-based disinfectant in environmental hygiene in terms of odor and corrosion, a disinfectant and a virus disinfectant excellent in workability are desired.
細菌やウイルスへ有効な薬剤として過酸化水素が知られている。過酸化水素は、実質的に無色無臭であり、分解して水と酸素になることから人や環境負荷の少ない薬剤として知られているが、３０〜６０秒程度の短時間でその除菌効果を得るためには高濃度を必要とする。例えば３％の過酸化水素水がオキシドールとして創傷、潰瘍部位の消毒に用いられており、また、欧米においては６％以上の濃度は医療機具の高水準消毒として用いられている。このような濃度では、過酸化水素は、刺激性、腐食性が大きいという欠点がある。 Hydrogen peroxide is known as an effective drug against bacteria and viruses. Hydrogen peroxide is substantially colorless and odorless and is known as a drug with little human and environmental impact because it decomposes into water and oxygen, but its disinfection effect in a short time of about 30 to 60 seconds In order to obtain a high concentration. For example, 3% hydrogen peroxide is used as oxidol for disinfection of wounds and ulcers, and in Europe and the United States, a concentration of 6% or more is used for high-level disinfection of medical devices. At such concentrations, hydrogen peroxide has the disadvantage of being highly irritating and corrosive.
このような欠点を改善するものとして、様々な混合剤が提案されているが、特に細菌、ウイルスに対する効力を向上させる組み合わせとして、過酸化水素とベンジルアルコールからなる消毒剤が提案されている（特許文献１）。これは、過酸化水素とベンジルアルコールの相乗効果により実質的に使用濃度を低減するものであるが、この除菌効果は、持続性が無いという欠点がある。そのため、環境表面の消毒に使用する場合、日に数度の消毒作業をしなければ、実質的な除菌、除ウイルス効果が得られず、施設の床面、壁面等の清掃には不適当であった。この他、過酸化水素と銀成分からなる消毒剤（特許文献２）や、過酸化水素、銀成分、過カルボン酸及びそれに対応するカルボン酸を含有する水性組成物が提案されている（特許文献３）。しかしこれらの消毒剤も即効性を得るためには過酸化水素を高濃度にしなければならず、刺激性、腐食性の面で更なる改善が求められている。 Various mixture agents have been proposed to remedy such drawbacks, and disinfectants consisting of hydrogen peroxide and benzyl alcohol have been proposed as a combination that improves the efficacy against bacteria and viruses. Reference 1). This substantially reduces the use concentration due to the synergistic effect of hydrogen peroxide and benzyl alcohol, but this sterilization effect has the disadvantage that it is not persistent. Therefore, when it is used for disinfection of the environmental surface, if it does not perform disinfection work several times a day, it cannot obtain a substantial sterilization and virus removal effect, and it is inappropriate for cleaning the floor and wall surfaces of facilities. Met. In addition, a disinfectant comprising hydrogen peroxide and a silver component (Patent Document 2), and an aqueous composition containing hydrogen peroxide, a silver component, a percarboxylic acid and a corresponding carboxylic acid have been proposed (Patent Document). 3). However, these disinfectants must also have a high concentration of hydrogen peroxide in order to obtain immediate effects, and further improvements are required in terms of irritation and corrosivity.
本発明は、このような事情に鑑み、各種施設等の床面、壁面等の処理、清掃に用いることに適した刺激性、腐食性の少ない、即効性及び残効性に優れた除菌、除ウイルス剤及び除菌、除ウイルス方法を提供することを課題とする。 In view of such circumstances, the present invention is sterilization suitable for use in cleaning, flooring and wall surfaces of various facilities, etc., cleaning, corrosiveness, sterilization excellent in immediate effect and residual effect, It is an object of the present invention to provide a virus eliminating agent, a sterilization method, and a virus eliminating method.
本発明を概説すれば、本発明は、
（１）過酸化水素、ベンジルアルコール及び銀成分を有効成分として含有することを特徴とする除菌、除ウイルス剤、
（２）過酸化水素、ベンジルアルコール及び銀成分が質量で、１０，０００〜３０，００００：１０，０００〜４００，０００：１〜１，０００の割合で含有する（１）項記載の除菌、除ウイルス剤、
（３）銀成分が銀塩である（１）項又は（２）項記載の除菌、除ウイルス剤及び、
（４）過酸化水素、ベンジルアルコール及び銀成分を使用する除菌、除ウイルス方法を提供するものである。In summary, the present invention provides:
(1) Disinfection and virus removal agent characterized by containing hydrogen peroxide, benzyl alcohol and silver component as active ingredients,
(2) Disinfection according to (1), wherein hydrogen peroxide, benzyl alcohol and silver component are contained in a mass ratio of 10,000 to 30,000: 10,000 to 400,000: 1 to 1,000. Antiviral agent,
(3) The sterilization, virus removal agent according to (1) or (2), wherein the silver component is a silver salt,
(4) To provide a sterilization and virus removal method using hydrogen peroxide, benzyl alcohol and silver components.
本発明者は、上記課題を解決するため鋭意検討を重ねた結果、過酸化水素、ベンジルアルコール及び銀成分を併用することで予想外に優れた除菌、除ウイルス効力を有することを見出し、更に処理表面に抗菌性が付与されることを見出し、本発明を完成するに至った。 As a result of intensive studies to solve the above-mentioned problems, the present inventor has found that by using hydrogen peroxide, benzyl alcohol and a silver component in combination, it has unexpectedly excellent sterilization and virus removal effects, and The inventors have found that antibacterial properties are imparted to the treated surface and have completed the present invention.
本発明の除菌、除ウイルス剤は、従来の過酸化水素系殺菌剤に比べ飛躍的な除菌、除ウイルス効果を有し、処理した表面に抗菌性が付与されという特性を併せ持つため持続性にも優れる。従って、既存の殺菌剤に比べ臭気、刺激性、腐食性が大きく改善されるだけでなく、除菌効果の持続性が改善されることにより消毒作業を軽減することができ、施設の床面、壁面等の除菌、除ウイルス剤として好適に使用することができる。 The sterilization and virus removal agent of the present invention has a dramatic sterilization and virus removal effect compared to conventional hydrogen peroxide-based disinfectants, and also has the property that antibacterial properties are imparted to the treated surface. Also excellent. Therefore, not only odor, irritation and corrosiveness are greatly improved compared to existing disinfectants, but also the disinfection work can be reduced by improving the sustainability of the sterilization effect, the floor surface of the facility, It can be suitably used as a sterilizing agent and a virus eliminating agent for walls and the like.
本発明の除菌、除ウイルス剤は、必須成分として過酸化水素、ベンジルアルコール及び銀成分及び溶媒を含有してなる。また、本発明の除菌、除ウイルス剤には、必要に応じて、安定剤、界面活性剤、防錆剤や他の殺菌剤を混合することもできる。本発明に使用する過酸化水素、ベンジルアルコール及び銀成分は、いずれも市販されており容易に入手することができる。過酸化水素は、広く市販されている有効成分として１０％〜５０％のものを使用することができる。ベンジルアルコールは、各種の化学工業分野で用いられているものが使用可能である。また、銀成分としては、硝酸銀、硫酸銀及び塩化銀等の一般的な銀塩、チオスルファイト銀錯体及び塩化銀ナトリウム錯体等の銀錯体、これらをゼオライト、シリカゲル、ケイ酸塩、リン酸塩類、溶解性ガラス、活性炭又は金属担体等に担持させたもの並びに１ｎｍ〜１μｍの範囲に微粒子化したコロイダル銀から選択することができる。溶媒としては、水が挙げられるが、塩素等の添加物のある水道水より浄化水、すなわち蒸留水や脱イオン水を用いるのが好ましい。 The sterilizing and antiviral agent of the present invention comprises hydrogen peroxide, benzyl alcohol, a silver component and a solvent as essential components. Moreover, a stabilizer, surfactant, a rust preventive agent, and another disinfectant can also be mixed with the bacteria elimination and virus elimination agent of this invention as needed. The hydrogen peroxide, benzyl alcohol and silver component used in the present invention are all commercially available and can be easily obtained. Hydrogen peroxide having 10% to 50% can be used as an active ingredient widely marketed. As benzyl alcohol, those used in various chemical industrial fields can be used. Examples of the silver component include general silver salts such as silver nitrate, silver sulfate and silver chloride, silver complexes such as thiosulfite silver complex and silver chloride sodium complex, and these include zeolite, silica gel, silicate, and phosphates. Further, it can be selected from those supported on soluble glass, activated carbon, a metal carrier or the like, and colloidal silver finely divided in the range of 1 nm to 1 μm. Examples of the solvent include water, but it is preferable to use purified water, that is, distilled water or deionized water, rather than tap water with additives such as chlorine.
本発明の除菌、除ウイルス剤で過酸化水素、ベンジルアルコール及び銀成分の配合割合は、質量で、１０，０００〜３００，０００：１０，０００〜４００，０００：１〜１，０００である。また、過酸化水素、ベンジルアルコール及び銀成分の使用時の濃度は、１，０００〜３０，０００ｐｐｍ、１，０００〜４０，０００ｐｐｍ及び銀として０．１〜１００ｐｐｍであり、好ましくは、２，０００〜１０，０００ｐｐｍ、５，０００〜３０，０００ｐｐｍ、銀として１〜５０ｐｐｍの銀成分である。これらの濃度は、除菌、除ウイルス剤として使用時に上記濃度となれば良く、従って高濃度品を作成し、使用時に適宜希釈して使用することもできる。 The mixing ratio of hydrogen peroxide, benzyl alcohol and silver component in the sterilization and virus removal agent of the present invention is 10,000 to 300,000: 10,000 to 400,000: 1 to 1,000 in mass. . Moreover, the density | concentration at the time of use of hydrogen peroxide, benzyl alcohol, and a silver component is 1,000-30,000 ppm, 1,000-40,000 ppm, and 0.1-100 ppm as silver, Preferably, it is 2,000. It is a silver component of 1 to 50 ppm as silver to 10,000 ppm, 5,000 to 30,000 ppm, and silver. These concentrations only have to be the above concentrations when used as a sterilizing and virus eliminating agent. Therefore, a high-concentration product can be prepared and used by appropriately diluting at the time of use.
過酸化水素、ベンジルアルコールと銀成分を混合する場合、希釈した過酸化水素にベンジルアルコール及び銀成分を加えることが好ましい。また、一液製剤化した場合、適当な安定剤を加えることが好ましい。安定剤としては、強鉱酸や有機酸が挙げられる。強鉱酸としては、リン酸、硝酸、塩酸、硫酸及びホウ酸等が好ましく、また有機酸としては、酒石酸、クエン酸、マレイン酸、マロン酸及び馬尿酸等が好ましい。これらの酸を加えてｐＨを酸性に保つことは、過酸化水素によるガスの発生を抑制する目的にも好ましい。 When hydrogen peroxide, benzyl alcohol and a silver component are mixed, it is preferable to add benzyl alcohol and a silver component to diluted hydrogen peroxide. In addition, when a one-component preparation is made, it is preferable to add an appropriate stabilizer. Stabilizers include strong mineral acids and organic acids. As the strong mineral acid, phosphoric acid, nitric acid, hydrochloric acid, sulfuric acid, boric acid and the like are preferable, and as the organic acid, tartaric acid, citric acid, maleic acid, malonic acid, hippuric acid and the like are preferable. The addition of these acids to keep the pH acidic is also preferable for the purpose of suppressing the generation of gas due to hydrogen peroxide.
本発明に使用できる界面活性剤としては、カチオン性界面活性剤、アニオン性界面活性剤、ノニオン性界面活性剤及び両面界面活性剤等が挙げられる。施設の床面、壁面等の清掃に用いる際には油汚れに対する洗浄力を付与するため、ノニオン性界面活性剤を配合することが好ましい。ノニオン系界面活性剤としては、高級アルコールエチレンオキシド付加物、高級アルキルアミン付加物、アルキルフェノール付加物、多価アルコール脂肪酸エステル付加物、プロピレンオキシド共重合体、多価アルコールアルキルエステル等が挙げられる。 Examples of the surfactant that can be used in the present invention include a cationic surfactant, an anionic surfactant, a nonionic surfactant, and a double-sided surfactant. When used for cleaning floors, wall surfaces, etc. of facilities, it is preferable to add a nonionic surfactant to impart detergency against oil stains. Nonionic surfactants include higher alcohol ethylene oxide adducts, higher alkylamine adducts, alkylphenol adducts, polyhydric alcohol fatty acid ester adducts, propylene oxide copolymers, polyhydric alcohol alkyl esters, and the like.
防錆剤としては、例えばアミノホスホン酸、そのナトリウム、カリウム、アンモニウム及びアルカノールアミン等塩、ヒドロキシエタンジホスホン酸、ジメチルアミノメタンジホスホン酸、これらのナトリウム、カリウム、アンモニウム及びアルカノールアミン塩、並びにこれらの混合物等が適している。１，２，３−ベンゾトリアゾールもまた銅や黄銅の防錆剤として適している。 Examples of rust preventives include aminophosphonic acid, its sodium, potassium, ammonium and alkanolamine salts, hydroxyethanediphosphonic acid, dimethylaminomethanediphosphonic acid, their sodium, potassium, ammonium and alkanolamine salts, and these A mixture of these is suitable. 1,2,3-benzotriazole is also suitable as a rust inhibitor for copper and brass.
本発明の除菌、除ウイルス剤を対象物品に接触させる手段に制限はなく、散布、清掃及び浸漬等が挙げられる。すなわち、モップを用いて床面に広げることや、スプレーで対象機器に直接噴霧することができ、処理表面を自然乾燥させることで抗菌性が付与され、持続的な除菌が可能となる。また、除菌、除ウイルス剤に洗浄用の界面活性剤を配合することで除菌と洗浄を同時に行なうことも可能である。 There is no restriction | limiting in the means to which the disinfection and the virus removal agent of this invention are made to contact target object, A spraying, cleaning, immersion, etc. are mentioned. That is, it can be spread on the floor using a mop or sprayed directly on the target device with a spray, and the treated surface is naturally dried to impart antibacterial properties and enable continuous sterilization. It is also possible to perform sterilization and washing at the same time by incorporating a detergent for washing into the sterilization and virus removal agent.
本発明は、また物品の除菌、除ウイルス方法に関する。すなわち、本発明は、過酸化水素、ベンジルアルコール及び銀成分を含む水溶液からなる除菌、除ウイルス剤を得、当該薬剤を物品に接触させることを特徴とする対象物品の除菌、除ウイルス方法も包含する。過酸化水素、ベンジルアルコール及び銀成分は、同時に又は別々に添加し、共存させることにより相乗的な除菌、ウイルス不活性効果を得ることもできる。除菌、除ウイルス対象物品は、特に制限がなく、食品工場、病院、養老施設、畜舎等の施設の床面、壁面、各種の機器等が挙げられる。別々に添加する場合も、希釈した過酸化水素に、ベンジルアルコールや銀成分を加えることが好ましい。 The present invention also relates to a method for sterilizing and erasing articles. That is, the present invention provides a sterilization / virus removal method for a target article characterized by obtaining a sterilization / virus removal agent comprising an aqueous solution containing hydrogen peroxide, benzyl alcohol and a silver component, and bringing the drug into contact with the article. Is also included. Hydrogen peroxide, benzyl alcohol and silver components can be added simultaneously or separately and allowed to coexist to obtain a synergistic sterilization and virus inactivation effect. The articles to be sterilized and eradicated are not particularly limited, and examples thereof include floors, wall surfaces, and various devices of facilities such as food factories, hospitals, nursing facilities, and barns. Also when adding separately, it is preferable to add benzyl alcohol and a silver component to the diluted hydrogen peroxide.
以下に、製剤例、比較例及び試験例等を挙げて本発明を更に詳細に説明するが、これらによりなんら本発明を限定するものではない。 Hereinafter, the present invention will be described in more detail with reference to formulation examples, comparative examples, and test examples, but the present invention is not limited thereto.
製剤例１
３５％過酸化水素、硝酸銀及びベンジルアルコールを用意し、これらの所定量を適宜混合し、水で希釈して除菌除ウイルス剤を得る。
製剤例２
３５％過酸化水素１４重量部、ベンジルアルコール２５重量部、硝酸銀０．０１５８重量部及びポリオキシエチレンアルキルエーテル（竹本油脂（株）製パイオニンＤ−１１０７）１重量部及び水９５９．９８４２重量部を配合して除菌除ウイルス剤を得、本品はリン酸でＰＨ３．０に調整する。Formulation Example 1
Prepare 35% hydrogen peroxide, silver nitrate, and benzyl alcohol, mix these predetermined amounts as appropriate, and dilute with water to obtain a sterilization virus removal agent.
Formulation Example 2
14 parts by weight of 35% hydrogen peroxide, 25 parts by weight of benzyl alcohol, 0.0158 parts by weight of silver nitrate, 1 part by weight of polyoxyethylene alkyl ether (Pionin D-1107 manufactured by Takemoto Oil & Fat Co., Ltd.) and 959.9842 parts by weight of water Mix to obtain a sterilizing virus product, and this product is adjusted to pH 3.0 with phosphoric acid.
比較例１
３５％過酸化水素１４重量部、ベンジルアルコール２５重量部、ポリオキシエチレンアルキルエーテル（竹本油脂（株）製パイオニンＤ−１１０７）１重量部及び水９９６０重量部を配合した後、リン酸でＰＨ３．０に調整する。Comparative Example 1
After blending 14 parts by weight of 35% hydrogen peroxide, 25 parts by weight of benzyl alcohol, 1 part by weight of polyoxyethylene alkyl ether (Pionine D-1107 manufactured by Takemoto Yushi Co., Ltd.) and 9960 parts by weight of water, PH3. Adjust to zero.
試験例１（殺菌力試験）
過酸化水素、ベンジルアルコール及び硝酸銀を滅菌した蒸留水で所定濃度に希釈した薬液、普通ブイヨン培地で１晩前培養したスタフィロコッカス・アウレウス菌液並びに２％チオ硫酸ナトリウム、０．５％レシチン及び１．５％ポリソルベート８０を含むニュートリエントブロス液体培地からなる中和液を用意した。薬液５０ｍｌに、菌液を、菌数が１０７ＣＦＵ／ｍｌ程度となるように添加接種し、室温で３０秒静置して接触させた。薬剤との接触を停止させるため、この薬液０．１ｍｌを中和液１０ｍｌに加えた。次に、生菌数の測定を容易にするため、この中和液を滅菌蒸留水で段階的に希釈して１０倍系列希釈液を作製し、それぞれ１ｍｌを標準寒天培地に接種した。３０℃で７２時間培養した後、生菌数（ＣＦＵ／ｍｌ）を測定した。結果を表１に示す。Test Example 1 (Bactericidal power test)
Chemical solution diluted with hydrogen peroxide, benzyl alcohol and silver nitrate to a predetermined concentration with sterilized distilled water, Staphylococcus aureus bacterial solution pre-cultured overnight in normal bouillon medium, 2% sodium thiosulfate, 0.5% lecithin and A neutralization solution consisting of a nutrient broth liquid medium containing 1.5% polysorbate 80 was prepared. The bacterial solution was added to and inoculated into 50 ml of the chemical solution so that the number of bacteria was about 10 7 CFU / ml, and left at room temperature for 30 seconds to contact. In order to stop the contact with the drug, 0.1 ml of this drug solution was added to 10 ml of the neutralized solution. Next, in order to facilitate the measurement of the number of viable bacteria, this neutralized solution was diluted stepwise with sterilized distilled water to prepare 10-fold series diluted solutions, and 1 ml each was inoculated on a standard agar medium. After culturing at 30 ° C. for 72 hours, the viable cell count (CFU / ml) was measured. The results are shown in Table 1.
表１より、本発明の除菌、除ウイルス剤は、それぞれの成分単独又は２成分の混合では、殺菌効果が得られなかった濃度においても、相乗的に優れた殺菌効果を示すことがわかる。 From Table 1, it can be seen that the sterilizing and antibacterial agents of the present invention show synergistically excellent bactericidal effects even at concentrations where the bactericidal effects could not be obtained by mixing each component alone or two components.
試験例２
（細胞増殖液の調製）
イーグルＭＥＭ培地（「ニッスイ」（１）日水製薬（株）製）に、牛胎児血清５％、グルタミン０．０３％、アンホテリシンＢ溶液０．１％及び抗生物質混合液１％の割合で加え、７．５％炭酸水素ナトリウム水溶液でｐＨを７．１〜７．４（３７℃）に調整したものを細胞増殖液とした。Test example 2
(Preparation of cell growth solution)
Added to Eagle MEM medium ("Nissui" (1) manufactured by Nissui Pharmaceutical Co., Ltd.) at a ratio of 5% fetal calf serum, 0.03% glutamine, 0.1% amphotericin B solution and 1% antibiotic mixture A cell growth solution was prepared by adjusting the pH to 7.1 to 7.4 (37 ° C.) with a 7.5% sodium hydrogen carbonate aqueous solution.
（ポリオウイルス浮遊液の調製）
細胞増殖液５０ｍｌを用いてＨｅＬａ細胞の培養を行なった後、ポリオウイルス浮遊液４００μｌを接種して、ＣＯ２インキュベーター（３７℃、湿度９０％以上、炭酸ガス濃度５％）でウイルスを増殖させた。細胞変性効果の出現後、凍結融解を行い、３０００ｒｐｍで５分間遠心分離して得られた上清をウイルス浮遊液とした。(Preparation of poliovirus suspension)
After culturing HeLa cells using 50 ml of cell growth solution, 400 μl of poliovirus suspension was inoculated, and the virus was propagated in a CO 2 incubator (37 ° C., humidity 90% or more, carbon dioxide concentration 5%). . After the appearance of cytopathic effect, it was frozen and thawed, and the supernatant obtained by centrifugation at 3000 rpm for 5 minutes was used as a virus suspension.
（ウイルス不活化試験法）
過酸化水素、ベンジルアルコール及び硝酸銀を、ＰＢＳを用いて所定濃度となるように希釈した薬液２５ｍｌに、ポリオウイルス浮遊液０．１ｍｌを添加し、攪拌しながら１０分間室温で反応させた。その後、０．１ｍｌを、２％チオ硫酸ナトリウム、０．２％レシチン及び１．５％ポリソルベート８０を含む細胞増殖液からなる中和液１０ｍｌへ加えて反応を停止させた。次いで、必要に応じて細胞増殖液を１０倍段階希釈し、単層培養を行なったＨｅＬａ細胞へ希釈した試験液０．２ｍｌを加え攪拌した後、ＣＯ２インキュベーター内で６０分間静置した。その後、メチルセルロース入りの細胞増殖液を３ｍｌずつ重層し、ＣＯ２インキュベーターで３日間培養した。１０％ホルマリンで固定した後、メチレンブルーを用いて染色し、感染価（ＰＦＵ／ｍｌ）を測定した。試験結果を表２に示す。(Virus inactivation test method)
0.1 ml of poliovirus suspension was added to 25 ml of a chemical solution obtained by diluting hydrogen peroxide, benzyl alcohol and silver nitrate to a predetermined concentration using PBS, and allowed to react at room temperature for 10 minutes with stirring. Thereafter, 0.1 ml was added to 10 ml of a neutralization solution consisting of a cell growth solution containing 2% sodium thiosulfate, 0.2% lecithin and 1.5% polysorbate 80 to stop the reaction. Then, if necessary, the cell growth solution was diluted 10-fold serially, 0.2 ml of the diluted test solution was added to HeLa cells subjected to monolayer culture, stirred, and then allowed to stand for 60 minutes in a CO 2 incubator. Thereafter, 3 ml of cell growth solution containing methylcellulose was layered and cultured in a CO 2 incubator for 3 days. After fixing with 10% formalin, staining was carried out with methylene blue, and the infectivity titer (PFU / ml) was measured. The test results are shown in Table 2.
表２より、本発明の除菌、除ウイルス剤は、各２成分の混合ではウイルスへの効果が得られなかった濃度においてウイルス不活性化効果が得られることがわかる。 From Table 2, it can be seen that the antibacterial and antiviral agent of the present invention provides a virus inactivating effect at a concentration at which mixing of the two components did not provide an effect on the virus.
試験例３
（試験片の調製）
プラスチックタイル（素材ＰＶＣ）を５ｃｍ角の正方形に切り取り、表面を洗剤及びエタノールで洗浄した後、乾燥させる。不織布のキムワイプにサンプル２ｍｌを染込ませ、タイル表面（表一面のみ）を細かな水滴が残る程度に拭く。その後クリーンベンチ内で自然乾燥させる。Test example 3
(Preparation of test piece)
A plastic tile (raw material PVC) is cut into a square of 5 cm square, and the surface is washed with a detergent and ethanol, and then dried. Dip 2 ml of sample into a non-woven Kimwipe and wipe the tile surface (only one surface) to the extent that fine water droplets remain. Then let it air dry in a clean bench.
（抗菌性試験法ＪＩＳ Ｚ ２８０１に準じる。）
５００倍に希釈したニュートリエントブロス液体培地中に、前培養したスタフィロコッカス・アウレウス菌を接種し、１０５程度になるよう供試菌液を調製した。前記試験片を、試験面を上にして滅菌済みシャーレ内に置き、供試菌液０．４ｍｌをシャーレ内の試験片に滴下した。滴下した試験菌液の上にポリエチレン製のフィルムを被せ、試験液がフィルム全体に行きわたるように軽く押さえた後シャーレに蓋をした。加工及び無加工試験片は室温に２４時間静置し、その後試験片を滅菌バッグに移し、ＳＣＤＬＰ培地１０ｍｌを加えてフィルムを十分に揉んで試験菌を洗い出した。洗い出し液を１０倍段階希釈して１ｍｌを標準寒天培地に接種し、３０℃で７２時間培養後、生菌数（ＣＦＵ／ｍｌ）を測定した。初期菌数は、２４時間静置前の無加工試験片の一つを洗い出し、同様の方法で標準寒天培地を用いて菌数を測定した。結果を表３に示す。結果は、下式で求めた値抗菌活性値で示す。
抗菌活性値＝１０ｇ（抗菌無加工試験片の２４時間後の生菌数／抗菌加工試験片の２４時間後の生菌数）(According to antibacterial test method JIS Z 2801.)
During nutrient broth liquid medium diluted to 500-fold, inoculated with Staphylococcus aureus bacteria were precultured, to prepare a test bacteria liquid so that about 10 5. The test piece was placed in a sterilized petri dish with the test surface facing up, and 0.4 ml of the test bacteria solution was dropped onto the test piece in the petri dish. A polyethylene film was placed on the dropped test bacterial solution, and lightly pressed so that the test solution would reach the entire film, and then the petri dish was covered. The processed and unprocessed test specimens were allowed to stand at room temperature for 24 hours, and then the test specimens were transferred to a sterilized bag, and 10 ml of SCDLP medium was added, and the test bacteria were washed out by thoroughly shaking the film. The washing solution was diluted 10-fold and 1 ml was inoculated on a standard agar medium. After culturing at 30 ° C. for 72 hours, the viable cell count (CFU / ml) was measured. The initial number of bacteria was determined by washing one untreated specimen before standing for 24 hours and measuring the number of bacteria using a standard agar medium in the same manner. The results are shown in Table 3. A result is shown by the value which was calculated | required by the following formula and the antibacterial activity value.
Antibacterial activity value = 10 g (viable count after 24 hours of antibacterial unprocessed test piece / viable count after 24 hours of antibacterial processed test piece)
ＪＩＳの評価によれば、抗菌加工製品の抗菌効果は、製品上の２４時間後の試験菌の生菌数が無加工製品上の生菌数の１％以下（抗菌活性値２．０以上）であることを求めているが、本発明の薬剤は、高い抗菌活性値を示し優れた抗菌性を付与していることがわかる。 According to JIS evaluation, the antibacterial effect of antibacterial processed products is that the number of viable bacteria of the test bacteria after 24 hours on the product is 1% or less of the number of viable bacteria on the unprocessed product (antibacterial activity value 2.0 or more) However, the drug of the present invention shows a high antibacterial activity value and imparts excellent antibacterial properties.
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| GB2479712A (en) * | 2010-04-08 | 2011-10-26 | 2Pure Products Ltd | Aqueous composition for suppressing malodour |
| WO2013085960A1 (en) * | 2011-12-05 | 2013-06-13 | Larson Brian G | Destruction of genetic material |
| US8834939B2 (en) | 2009-02-13 | 2014-09-16 | Probe Industries Limited | Aqueous compositions of copper salts and hydrogen peroxide |
| JP2015097989A (en) * | 2013-11-19 | 2015-05-28 | 昭和電工株式会社 | Virus removing agent and virus removing method |
| JP2016002384A (en) * | 2014-06-18 | 2016-01-12 | ｎ−ｔｅｃｈ株式会社 | Antibacterial/deodorant composition |
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| JPH11107033A (en) * | 1997-09-30 | 1999-04-20 | Kenji Nakamura | Antimicrobial cellulose fiber and its production |
| JP2001288020A (en) * | 1999-04-23 | 2001-10-16 | Sumika Agrotech Co Ltd | Antimicrobial agent and method for producing the same |
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| JPH11107033A (en) * | 1997-09-30 | 1999-04-20 | Kenji Nakamura | Antimicrobial cellulose fiber and its production |
| JP2001288020A (en) * | 1999-04-23 | 2001-10-16 | Sumika Agrotech Co Ltd | Antimicrobial agent and method for producing the same |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US8834939B2 (en) | 2009-02-13 | 2014-09-16 | Probe Industries Limited | Aqueous compositions of copper salts and hydrogen peroxide |
| US9700646B2 (en) | 2009-02-13 | 2017-07-11 | Probe Industries Limited | Compositions and their use |
| US10940228B2 (en) | 2009-02-13 | 2021-03-09 | 2Pure Products Limited | Aqueous solutions of copper salts and hydrogen peroxide |
| GB2479712A (en) * | 2010-04-08 | 2011-10-26 | 2Pure Products Ltd | Aqueous composition for suppressing malodour |
| WO2013085960A1 (en) * | 2011-12-05 | 2013-06-13 | Larson Brian G | Destruction of genetic material |
| JP2015097989A (en) * | 2013-11-19 | 2015-05-28 | 昭和電工株式会社 | Virus removing agent and virus removing method |
| JP2016002384A (en) * | 2014-06-18 | 2016-01-12 | ｎ−ｔｅｃｈ株式会社 | Antibacterial/deodorant composition |
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