JP2009011163A - Antioxidant food - Google Patents
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Description
本発明は、緑色植物由来の素材を天然水で懸濁して得られた組成物に関する。 The present invention relates to a composition obtained by suspending a green plant-derived material in natural water.
近年、緑色植物由来の素材は、コレステロールの吸収抑制、有害物質の吸着、食後血糖値の急上昇防止、および抗高血圧予防に効果があることが知られている（特許文献１）。その中でも、緑色植物由来の素材に含まれるポリフェノール（カテキン、フラボン等）は、スーパーオキサイドディスムターゼ（ＳＯＤ）様活性を有することが知られている（特許文献２）。そのため、ポリフェノールを含む緑茶、青汁等の食品が健康食品として注目を集めている。 In recent years, it has been known that green plant-derived materials are effective in suppressing absorption of cholesterol, adsorbing harmful substances, preventing rapid increase in postprandial blood glucose level, and preventing antihypertension (Patent Document 1). Among them, polyphenols (catechin, flavone, etc.) contained in green plant-derived materials are known to have superoxide dismutase (SOD) -like activity (Patent Document 2). Therefore, foods such as green tea and green juice containing polyphenol are attracting attention as health foods.
しかしながら、消費者の多くは、緑色植物由来の素材を水道水に懸濁し、食している。また、緑色植物由来の素材に含まれるポリフェノールは、水道水では十分に抽出されない。そのため、緑色植物由来の素材のポリフェノールは、体内の腸管内では吸収されず、排出されるという問題がある。 However, many consumers suspend and eat green plant-derived materials in tap water. Moreover, the polyphenol contained in the green plant-derived material is not sufficiently extracted with tap water. Therefore, there is a problem that the polyphenol, which is a green plant-derived material, is not absorbed in the intestinal tract of the body and is discharged.
そこで、緑色植物のポリフェノールを高濃度で抽出可能な天然水を使用した、組成物の開発が求められている。
本発明は、緑色植物由来の素材を天然水で懸濁して得られた、抗酸化に優れた組成物を提供することを目的とする。 An object of this invention is to provide the composition excellent in the antioxidant obtained by suspending the raw material derived from a green plant with natural water.
本発明者らは、上記目的を達成すべく鋭意検討した結果、緑色植物由来の素材を天然水で懸濁することにより、抗酸化に優れた組成物を見出した。 As a result of intensive studies to achieve the above object, the present inventors have found a composition excellent in antioxidation by suspending a green plant-derived material in natural water.
すなわち本発明は、緑色植物由来の素材を天然水で懸濁して得られた組成物に関する。 That is, the present invention relates to a composition obtained by suspending a green plant-derived material in natural water.
好ましくは、前記緑色植物由来の素材が、大麦、ケール、甘藷および茶からなる群より選択される一または二以上である、上記の組成物に関する。 Preferably, the green plant-derived material is one or more selected from the group consisting of barley, kale, sweet potato, and tea.
さらに好ましくは、前記天然水が、日本アルプスの地下水脈から取水した硬度１６ｍｇ／Ｌ及びｐＨ６．８であることを特徴とする、上記の組成物に関する。 More preferably, the natural water has a hardness of 16 mg / L and a pH of 6.8 taken from a groundwater vein in the Japanese Alps.
本発明によれば、緑色植物由来の素材を、硬度１６ｍｇ／ＬおよびｐＨ６．８の天然水で抽出することにより、抗酸化に優れた食品組成物を提供することができる。 ADVANTAGE OF THE INVENTION According to this invention, the food composition excellent in antioxidant can be provided by extracting the raw material derived from a green plant with the natural water of hardness 16mg / L and pH6.8.
以下、本発明の実施形態について説明する。なお、本発明は、下記実施形態の記載により限定して解釈するべきでなく、特許請求の範囲における記載の範囲内で種々の変更が可能である。 Hereinafter, embodiments of the present invention will be described. In addition, this invention should not be limited and interpreted by description of the following embodiment, A various change is possible within the range of description in a claim.
本発明で使用する緑色植物由来の素材としては、イネ科植物、アブラナ科植物、ヒルガオ科植物、ツバキ科植物などが挙げられる。これらの素材に含まれる食物繊維には体内の老廃物を排出する効果が期待できる。食物繊維を含有する食品組成物を摂取することにより、体内浄化効果が期待でき、さらには便通改善効果、肌荒れ改善効果などが期待できる。 Examples of the green plant-derived material used in the present invention include gramineous plants, cruciferous plants, convolvulaceae plants, and camellia plants. The dietary fiber contained in these materials can be expected to excrete waste products in the body. By ingesting a food composition containing dietary fiber, a body purification effect can be expected, and a bowel movement improving effect, a rough skin improving effect, and the like can be expected.
本発明に使用されるイネ科植物としては、大麦、小麦、ライ麦、燕麦などが挙げられる。これらのイネ科植物の若葉を乾燥して粉末化した乾燥粉末、乾燥前に圧搾して得られた搾汁、このような搾汁を乾燥した搾汁乾燥粉末または有機溶媒で抽出して得られた抽出物などが使用される。 Examples of the Gramineae plant used in the present invention include barley, wheat, rye, and buckwheat. Dry powder obtained by drying and pulverizing the young leaves of these grasses, squeezed by pressing before drying, obtained by extracting such squeezed dry powder or organic solvent Extract is used.
本発明に使用されるアブラナ科植物としては、キッチンケール、ツリーケール、ブッシュケール、マローケール、コラードおよび緑葉カンランなどが挙げられる。これらのアブラナ科植物の若葉を乾燥して粉末化した乾燥粉末、乾燥前に圧搾して得られた搾汁、このような搾汁を乾燥した搾汁乾燥粉末または有機溶媒で抽出して得られた抽出物などが使用される。 Examples of the cruciferous plant used in the present invention include kitchen kale, tree kale, bush kale, mallow kale, collard, and green leaf kanran. Dry powder obtained by drying and pulverizing young leaves of these cruciferous plants, squeezed by pressing before drying, obtained by extracting such squeezed dry dried powder or organic solvent Extract is used.
本発明に使用されるヒルガオ科植物としては、すいおう、ジョイホワイト、コガネセンガン、シロユタカ、サツマスターチおよびアヤムラサキなどが挙げられる。これらのヒルガオ科植物の若葉を乾燥して粉末化した乾燥粉末、乾燥前に圧搾して得られた搾汁、このような搾汁を乾燥した搾汁乾燥粉末または有機溶媒で抽出して得られた抽出物などが使用される。 Examples of the Convolvulaceae plant used in the present invention include Suio, Joy White, Koganesengan, Shirotaka, Satsumasuchi and Ayamurasaki. Dry powder obtained by drying and pulverizing the young leaves of these convolvulaceae plants, squeezed by squeezing before drying, obtained by extracting such squeezed dry dried powder or organic solvent Extract is used.
本発明に使用されるツバキ科植物としては、茶樹（学名：Camellia sinensis ）が挙げられる。これらのツバキ科植物の若葉を乾燥して粉末化した乾燥粉末、乾燥前に圧搾して得られた搾汁、このような搾汁を乾燥した搾汁乾燥粉末または有機溶媒で抽出して得られた抽出物などが使用される。 Examples of camellia plants used in the present invention include tea trees (scientific name: Camellia sinensis). Dry powder obtained by drying and pulverizing the young leaves of these camellia plants, squeezed by squeezing before drying, obtained by extracting such squeezed dry dried powder or organic solvent Extract is used.
本発明で使用する天然水としては、中央アルプスの地下１，５００ｍの水脈から汲み上げた天然水が挙げられる。これらの天然水が硬度１６ｍｇ／ＬおよびｐＨ６．８であることを特徴とする非加熱ろ過方式によって汲み上げた天然水として使用される。 Examples of natural water used in the present invention include natural water pumped from a 1,500 m underground water vein in the Central Alps. These natural waters are used as natural water pumped by a non-heated filtration system characterized by a hardness of 16 mg / L and pH 6.8.
以下、本発明の実施例について説明する。なお、本発明は、下記の実施例に限定して解釈すべきではなく、特許請求の範囲における記載の範囲内で種々の変更が可能である。 Examples of the present invention will be described below. It should be noted that the present invention should not be construed as being limited to the following examples, and various modifications can be made within the scope of the claims.
（実施例１：ポリフェノール抽出の検証）
緑色植物由来の素材と飲食可能な天然水を使用し、以下の方法によりポリフェノールの抽出を検証した。
(Example 1: Verification of polyphenol extraction)
The extraction of polyphenols was verified by the following method using green plant-derived materials and natural water that can be consumed.
大麦若葉末３．０ｇに硬度１６ｍｇ／ＬおよびｐＨ６．８であることを特徴とする天然水１００ｍｌで懸濁し、薄型多連式スターラーで２時間攪拌した。次に、５０ｍｌ遠沈管に懸濁液を４０ｍｌ移し、３，５００ｒｐｍで１０分間遠心分離を行った。そして、得られた上清を蒸留水で３倍希釈しサンプルとした。さらに、サンプル５００μＬとＦｏｌｉｎ試薬５００μＬを混合し、３分間静置した。その後、１０％炭酸ナトリウム溶液に加え、１時間静置後、３，５００ｒｐｍで２０分間遠心し、上清を７３０ｎｍにおける吸光度で測定した。結果を表１に示す。 Suspended with 100 ml of natural water characterized by having a hardness of 16 mg / L and pH 6.8 on 3.0 g of barley young leaf powder, and stirred for 2 hours with a thin multiple stirrer. Next, 40 ml of the suspension was transferred to a 50 ml centrifuge tube and centrifuged at 3,500 rpm for 10 minutes. The obtained supernatant was diluted 3 times with distilled water to prepare a sample. Further, 500 μL of sample and 500 μL of Folin reagent were mixed and allowed to stand for 3 minutes. Thereafter, in addition to a 10% sodium carbonate solution, the mixture was allowed to stand for 1 hour, centrifuged at 3,500 rpm for 20 minutes, and the supernatant was measured by absorbance at 730 nm. The results are shown in Table 1.
（比較例１：ポリフェノール抽出の検証）
大麦若葉末３．０ｇを硬度及びｐＨが異なるそれぞれの天然水１００ｍｌで懸濁し、薄型多連式スターラーで２時間攪拌した。次に、５０ｍｌ遠沈管に懸濁液を４０ｍｌ移し、３，５００ｒｐｍで１０分間遠心分離を行った。そして、得られた上清をＤＷで３倍希釈しサンプルとした。さらに、サンプル５００μＬとＦｏｌｉｎ試薬５００μＬを混合し、３分間静置した。その後、１０％炭酸ナトリウム溶液に加え、１時間静置後、３，５００ｒｐｍで２０分間遠心し、上清を７３０ｎｍにおける吸光度で測定した。結果を表１に示す。
(Comparative Example 1: Verification of polyphenol extraction)
3.0 g of barley young leaf powder was suspended in 100 ml of natural water of different hardness and pH, and stirred with a thin multiple stirrer for 2 hours. Next, 40 ml of the suspension was transferred to a 50 ml centrifuge tube and centrifuged at 3,500 rpm for 10 minutes. The obtained supernatant was diluted 3 times with DW to prepare a sample. Further, 500 μL of sample and 500 μL of Folin reagent were mixed and allowed to stand for 3 minutes. Thereafter, in addition to a 10% sodium carbonate solution, the mixture was allowed to stand for 1 hour, centrifuged at 3,500 rpm for 20 minutes, and the supernatant was measured by absorbance at 730 nm. The results are shown in Table 1.
表１の結果より、比較例１〜４に比べて、実施例のポリフェノールが有意に抽出されていることを示す。 From the results in Table 1, it is shown that the polyphenols of the examples are significantly extracted as compared with Comparative Examples 1 to 4.
（実施例２：ＳＯＤ様活性の測定）
大麦若葉末３．０ｇを硬度１６ｍｇ／ＬおよびｐＨ６．８であることを特徴とする天然水１００ｍｌに懸濁し、薄型多連式スターラーで２時間攪拌した。次に、９６ウェルプレートに７．５μＬを摂取し、発色試薬７５μＬおよび酵素液を７５μＬ加えて、３７℃で２０分間インキュベートを行った。さらに、反応停止液を１５０μＬ加えて反応を停止させ、よく攪拌後、５６０ｎｍにおける吸光度で測定した。結果を表２に示す。
(Example 2: Measurement of SOD-like activity)
3.0 g of barley young leaf powder was suspended in 100 ml of natural water characterized by a hardness of 16 mg / L and pH 6.8, and stirred with a thin multiple stirrer for 2 hours. Next, 7.5 μL was taken into a 96-well plate, 75 μL of a coloring reagent and 75 μL of enzyme solution were added, and incubation was performed at 37 ° C. for 20 minutes. Furthermore, 150 μL of a reaction stop solution was added to stop the reaction, and after stirring well, the absorbance at 560 nm was measured. The results are shown in Table 2.
（比較例２：ＳＯＤ様活性の測定）
大麦若葉末３．０ｇを硬度及びｐＨが異なるそれぞれの天然水１００ｍｌに懸濁し、薄型多連式スターラーで２時間攪拌した。次に、９６ウェルプレートに７．５μＬを摂取し、発色試薬７５μＬおよび酵素液を７５μＬ加えて、３７℃で２０分間インキュベートを行った。さらに、反応停止液を１５０μＬ加えて反応を停止させ、よく攪拌後、５６０ｎｍにおける吸光度で測定した。結果を表２に示す。
(Comparative Example 2: Measurement of SOD-like activity)
3.0 g of barley young leaf powder was suspended in 100 ml of natural water with different hardness and pH, and stirred with a thin multiple stirrer for 2 hours. Next, 7.5 μL was taken into a 96-well plate, 75 μL of a coloring reagent and 75 μL of enzyme solution were added, and incubation was performed at 37 ° C. for 20 minutes. Furthermore, 150 μL of a reaction stop solution was added to stop the reaction, and after stirring well, the absorbance at 560 nm was measured. The results are shown in Table 2.
表２の結果より、比較例１〜４に比べて、実施例のＳＯＤ様活性が有意に高いことを示す。 From the results of Table 2, it is shown that the SOD-like activity of the examples is significantly higher than those of Comparative Examples 1 to 4.
本発明によれば、緑色植物由来の素材を日本アルプスから取水した硬度１６ｍｇ／ＬおよびｐＨ６．８の天然水を用いることにより、多くのポリフェノールを抽出でき、そのうえ、抗酸化力が強い組成物を提供することができる。
According to the present invention, by using natural water having a hardness of 16 mg / L and a pH of 6.8 obtained from a green plant-derived material from the Japanese Alps, it is possible to extract many polyphenols, and in addition, a composition having a strong antioxidant power Can be provided.
Claims (3)
The composition according to claim 1 or 2, wherein the natural water has a hardness of 16 mg / L and a pH of 6.8 taken from a groundwater vein in the Japanese Alps.
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Citations (11)
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| JPH08231369A (en) * | 1995-02-20 | 1996-09-10 | Shiseido Co Ltd | Cosmetic |
| JPH10304853A (en) * | 1997-05-07 | 1998-11-17 | Daichiyuu:Kk | Production of health food and drink |
| JP2000060506A (en) * | 1998-08-21 | 2000-02-29 | Ogiwara Fumitake | Composition of highly concentrated mineral solution for addition and its production |
| JP2002034525A (en) * | 2000-07-24 | 2002-02-05 | Kazuhiro Kotani | Extract drink using marine deep water |
| JP2004215578A (en) * | 2003-01-15 | 2004-08-05 | Junko Sendo | Composition for health food product and health food product or health beverage product |
| JP2005087894A (en) * | 2003-09-18 | 2005-04-07 | Goshu Yakuhin Kk | Hardness-regulated natural water |
| JP2005204598A (en) * | 2004-01-23 | 2005-08-04 | Yamamoto Kanpo Seiyaku Kk | Young leaf powder of gramineous plant and method for producing the same |
| JP2005278509A (en) * | 2004-03-30 | 2005-10-13 | Fuji Keiki:Kk | Method for producing functional beverage |
| JP2005318866A (en) * | 2004-05-11 | 2005-11-17 | Toyo Shinyaku:Kk | Method for producing tea beverage and method for improving flavor of tea beverage |
| JP2005348626A (en) * | 2004-06-08 | 2005-12-22 | Takashi Omori | Healthy drinking water and method for producing the same |
| JP2006288366A (en) * | 2005-04-08 | 2006-10-26 | Joho Kagaku Kenkyusho:Kk | Reduced colloid percolating method for drink tea, reduced colloid tea, and production system for the colloid tea |
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2007
- 2007-06-29 JP JP2007172943A patent/JP2009011163A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08231369A (en) * | 1995-02-20 | 1996-09-10 | Shiseido Co Ltd | Cosmetic |
| JPH10304853A (en) * | 1997-05-07 | 1998-11-17 | Daichiyuu:Kk | Production of health food and drink |
| JP2000060506A (en) * | 1998-08-21 | 2000-02-29 | Ogiwara Fumitake | Composition of highly concentrated mineral solution for addition and its production |
| JP2002034525A (en) * | 2000-07-24 | 2002-02-05 | Kazuhiro Kotani | Extract drink using marine deep water |
| JP2004215578A (en) * | 2003-01-15 | 2004-08-05 | Junko Sendo | Composition for health food product and health food product or health beverage product |
| JP2005087894A (en) * | 2003-09-18 | 2005-04-07 | Goshu Yakuhin Kk | Hardness-regulated natural water |
| JP2005204598A (en) * | 2004-01-23 | 2005-08-04 | Yamamoto Kanpo Seiyaku Kk | Young leaf powder of gramineous plant and method for producing the same |
| JP2005278509A (en) * | 2004-03-30 | 2005-10-13 | Fuji Keiki:Kk | Method for producing functional beverage |
| JP2005318866A (en) * | 2004-05-11 | 2005-11-17 | Toyo Shinyaku:Kk | Method for producing tea beverage and method for improving flavor of tea beverage |
| JP2005348626A (en) * | 2004-06-08 | 2005-12-22 | Takashi Omori | Healthy drinking water and method for producing the same |
| JP2006288366A (en) * | 2005-04-08 | 2006-10-26 | Joho Kagaku Kenkyusho:Kk | Reduced colloid percolating method for drink tea, reduced colloid tea, and production system for the colloid tea |
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