JP2008507261A5 - - Google Patents

Claims (39)

An isolated polynucleotide comprising a nucleic acid sequence having at least about 85% identity with any one of SEQ ID NOs: 20-21 . The polynucleotide of claim 1, wherein the nucleic acid sequence has at least about 90% identity with any one of SEQ ID NOs: 20-21 . The polynucleotide of claim 2 wherein the nucleic acid sequence has at least about 95% identity with any one of SEQ ID NOs: 20-21. The polynucleotide according to claim 3, wherein the nucleic acid sequence is that described in any one of SEQ ID NOs: 20 to 21. The nucleic acid sequence encodes a polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs: 1299 to 1300;
The polynucleotide according to any one of claims 1 to 4. The nucleic acid sequence encodes a polypeptide comprising consecutive amino acids having at least about 85% homology with a polypeptide having the amino acid sequence set forth in any one of SEQ ID NOS: 1299-1300. 5. The polynucleotide according to any one of 4. The nucleic acid sequence encodes a polypeptide comprising consecutive amino acids having at least about 90% homology with a polypeptide having the amino acid sequence set forth in any one of SEQ ID NOS: 1299-1300. The polynucleotide described. 8. The nucleic acid sequence encodes a polypeptide comprising consecutive amino acids having at least about 95% homology with a polypeptide having the amino acid sequence set forth in any one of SEQ ID NOs: 1299-1300. The polynucleotide described. The polynucleotide according to any one of claims 1 to 4,
The nucleic acid sequence encodes a polypeptide comprising consecutive amino acids having at least about 85% homology with amino acids 128-142 of SEQ ID NO: 1300; or
The nucleic acid sequence encodes a polypeptide comprising consecutive amino acids having at least about 90% homology with amino acids 128-142 of SEQ ID NO: 1300; or
The nucleic acid sequence encodes a polypeptide comprising consecutive amino acids having at least about 95% homology with amino acids 128-142 of SEQ ID NO: 1300; or
The nucleic acid sequence encodes a polypeptide comprising amino acids 128-142 of SEQ ID NO: 1300;
The polynucleotide. Comprising a nucleic acid sequence complementary to the nucleic acid sequence of any one of claims 1 to 9; or
Comprising a nucleic acid sequence that hybridizes under stringent conditions to the polynucleotide sequence of any one of claims 1-9.
Isolated polynucleotide. A polypeptide having an amino acid sequence encoded by the nucleic acid sequence according to any one of claims 1 to 9. A polypeptide having an amino acid sequence at least about 85% homologous to the amino acid sequence set forth in any one of SEQ ID NOs: 1299-1300. 13. The polypeptide of claim 12, wherein the amino acid sequence is at least about 90% homologous to the amino acid sequence set forth in any one of SEQ ID NOs: 1299-1300. 14. The polypeptide of claim 13, wherein the amino acid sequence is at least about 95% homologous to the amino acid sequence set forth in any one of SEQ ID NOs: 1299-1300. The polypeptide of claim 14, comprising the amino acid sequence set forth in any one of SEQ ID NOs: 1299-1300. A polypeptide comprising consecutive amino acids having at least about 90% homology with amino acids 128-142 of SEQ ID NO: 1300. 17. The polypeptide of claim 16, having amino acids 128-142 of SEQ ID NO: 1300. A polypeptide comprising a first amino acid sequence that is at least about 90% homologous to SEQ ID NO: 1300 1-127 and a second amino acid sequence that is at least about 85% homologous to amino acids 128-142 of SEQ ID NO: 1300. And
The first amino acid sequence and the second amino acid sequence are contiguous and in sequence;
Said polypeptide. The first amino acid sequence is at least about 95% homologous to amino acids 1-127 of SEQ ID NO: 1300 and the second amino acid sequence is at least about 90% homologous to amino acids 128-142 of SEQ ID NO: 1300 The polypeptide according to claim 18. An expression vector comprising the polynucleotide sequence according to any one of claims 1-9. A host cell comprising the vector of claim 20. A method for producing a polypeptide comprising the steps of:
Culturing the host cell of claim 21 under conditions suitable for producing the polypeptide encoded by the polynucleotide, and recovering the polypeptide.
The above method. An antibody that binds to at least one epitope of a polypeptide having the amino acid sequence according to any one of claims 11 to 19. An isolated polynucleotide segment comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 335-339. A nucleic acid sequence complementary to any one of the polynucleotide segments of claim 24; or
A nucleic acid sequence that hybridizes under stringent conditions with any one of the polynucleotide segments of claim 24,
Isolated polynucleotide segment. A kit for detecting different expression of at least one polypeptide according to any one of claims 11 to 19 or at least one polypeptide according to any one of claims 1 to 9, 24 or 25. Kit for detecting lung cancer. 26. The kit comprises NAT-based technology, and the kit is capable of selectively hybridizing to a polynucleotide according to any one of claims 1-9, 24 or 25. The kit according to claim 26, further comprising: 28. The kit of claim 27, wherein the at least one primer pair amplifies an amplicon having SEQ ID NO: 1684. 29. A kit according to claim 27 or 28 comprising a pair of isolated oligonucleotides having SEQ ID NOs: 1646 and 1647. 27. The kit of claim 26, wherein the kit comprises at least one oligonucleotide capable of selectively hybridizing to the polynucleotide of any one of claims 1-9, 24 or 25. 27. The kit of claim 26, wherein the kit comprises the antibody of claim 23 and the kit further comprises at least one reagent for performing an immunoassay. 32. The immunoassay is selected from the group consisting of ELISA, RIA (radioimmunoassay), slot blot, immunohistochemical analysis, FACS (fluorescence activated cell sorting), radioimaging assay or western blot. Kit. Detecting the differential expression of at least one polypeptide according to any one of claims 11 to 19 or at least one polypeptide according to any one of claims 1 to 9, 24 or 25. , Lung cancer detection method. 34. The method of claim 33, wherein the step of detecting the different expression of a polypeptide is performed by the antibody of claim 23. 35. Performing a method according to claim 33 or 34 or using a kit according to any one of claims 26-32 in a sample obtained from a subject and / or disease progression of lung cancer and / or Or an in vitro method of monitoring therapeutic efficacy and / or detection of acute or chronic exacerbations. In selecting a lung cancer treatment comprising performing a method according to claim 33 or 34 or using a kit according to any one of claims 26-32 in a sample obtained from a subject. Vitro method. 37. The kit or method according to any one of claims 26 to 36, wherein the lung cancer comprises one or more of small cell or non-small cell lung cancer. 38. The kit of claim 37, wherein the non-small cell lung cancer comprises at least one of adenocarcinoma, squamous cell carcinoma and large cell carcinoma. The kit or method according to any one of claims 26 to 38, wherein lung cancer is detected by using a sample, the sample comprising blood, serum, plasma, blood cells, urine, sputum, saliva, Any feces, cerebrospinal fluid or CSF, lymph fluid, skin exocrine secretions, airways, intestinal tract, and urogenital tract, tears, nerve tissue, lung tissue, any human organ or tissue, optionally any obtained by lavage of the bronchial system A kit or method as described above, comprising one or more of a sample or cell culture component.