JP2008301807A - Array of nucleotide sequence and method of using the same for detection and identification of gene encoding protein having biotechnology-related activity presented in microbiological sample - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for rapidly and simultaneously identifying the presence of a gene encoding a protein having biotechnology-related activity in a microbiological sample. <P>SOLUTION: Disclosed are an array of nucleotide sequence and a method for using the array for the identification of a gene. Especially disclosed is a gene encoding a protein associated with biofilm formation, carbon dioxide fixation, energy metabolism, chemotaxis and motility, oxidation of iron, nitrogen metabolism, sulfur anabolism, oxidation and reduction of sulfide. The array of nucleotide sequence is provided as a useful tool in biotechnology in every case where the evaluation of characteristics of microbiological community is needed. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

Scope of the present invention

The present invention provides an array of nucleotide sequences for the rapid and simultaneous identification of the presence of a specific gene encoding a protein for activity in biotechnology present in a microbiological sample, as well as identifying the array in the identification of said gene. Relates to the method used. Specifically related to biofilm formation, CO ₂ fixation, energy metabolism, chemiotaxis and mobility, iron oxidation, nitrogen metabolism, sulfur assimilation, and oxidation / reduction of sulfide compounds A gene encoding the protein to be identified is identified. The array of nucleotide sequences is provided as a useful tool in biotechnology whenever it is necessary to assess the quality of the microbiological community.

Background information of the present invention

  Biotechnology generally refers to biological systems (such as complex microbiological communities), organisms (microorganisms, animals or plants) or their factions in the creation or modification of products or processes for specific uses. Any technical application that uses organisms (metabolites, proteins, nucleic acids). The present invention focuses on biotechnology using microorganisms, particularly on biomining and microbiological bioremediation.

  Many processes of microbiological biotechnology are in the base being treated, such as communities present in ores in the case of biomining, or communities present in contaminated soil or water in the case of bioremediation. Reinforce the development of the microbiological community that originally exists in the world. In those cases, having a tool that makes it possible to assess the metabolic characteristics of an existing community is, for example, whether the process we are interested in is feasible by the existing community, Alternatively, it is very useful in determining whether other appropriate strains should be seeded.

  To date, the most common approaches used to evaluate the microbiological community have been selective culture, fluorescence in situ hybridization (FISH), detection probes, polymerase chain reaction (PCR) and macro and micro DNA arrays. The identification of existing species by various techniques such as binding using such specific markers. Of the above techniques, those belonging to molecular biology, ie FISH, detection probes, PCR, and macro and micro DNA arrays are all DNA of unknown samples and microorganisms or microorganisms that are required to be detected or identified Based on hybridization with known sequences specific to a group, for example a species or genus.

  The problem with the above approach is that there are many different types of microorganisms that can exist in their microbiological community, so many tests have been performed to establish their composition, and then The acquired data must be correlated with the characteristics of each identified taxon to ultimately verify whether the function of interest is present in the sample. In the presence of microorganisms not previously described, no correlation can be made, even if their metabolic activity is known and important to the process. Therefore, a need arises for a method that directly assesses and controls the metabolic characteristics of the microbiological community and does not identify the microorganisms that comprise it.

  The technical problem is to create an array of nucleotide sequences and a method of using the array for detecting and identifying a gene encoding a protein having a biotechnology-related activity present in a microbiological sample By the method of the present invention.

  In changing the focus of the approach from the taxonomics of the communities studied to the identification of proteins related to the activities present in the communities, we have identified the essence of biotechnological processes-the metabolism that fundamentally executes those processes. Direct focus on function. For example, whenever the presence of a Leptospirillum spp. Is explored for a bioleaching process, what is actually explored is iron oxidation, which is a microorganism associated with a biotechnological process. The same applies to each of the above.

The new approach we designed requires biotechnological process operators: does this community oxidize iron? Oxidize sulfur? To fix CO ₂ ? Do you fix nitrogen? It is possible to respond accurately to; According to the existing approach to the technology, the operator first knows which species are present, and then he or she can answer this same question and the respective characteristics of those species to answer the same question. Must be related.

  In each case, a DNA fragment was designed to identify a gene encoding a protein with an activity related to biotechnology depending on the basic conditions: the specificity of the fragment corresponds only to the target gene. There must be. On the other hand, whenever it is possible, a search is made for regions that are specific for each target gene and that are conserved in orthologues. This is based on the idea of detecting not only the genes that have been sequenced but also the orthologous genes of each target gene that are not described. The results of this search correspond to fragments that are specific for a gene or function within a specific taxon in a specific region.

  With these designed DNA fragments, we are able to carry out the identification of the presence of those genes encoding proteins with biotechnology-related activity that are present in microbiological samples, A fragment array was developed.

  A good definition of a DNA array is that proposed by Schena and colleagues: “Microscopic and systematic arrangement of nucleic acids to allow simultaneous analysis of complex DNA samples” (Schena M., Heller, RA, Theriault, P., Konrad, K., Lachenmeier, E. and Davis, RW Trends Biotechnol. 16, 301-306, 1998).

  Depending on the diameter of the printed DNA dot, there are two types of arrays: macroarrays (300 microns and above) and microarrays (less than 100 microns). The former can be made manually in the laboratory and the dots can be visualized without the aid of special equipment. The latter requires an automated printing system (usually a robot printing platform) and specialized acquisition and image processing equipment.

  In the present invention, a DNA fragment array comprises an aligned system of dots printed on a flat surface such as glass, silicon or nylon sheets, each dot being known and of interest in biotechnology. It contains large copies of DNA fragments that are specific for certain genes.

  Detection methods using a DNA fragment array include simultaneous hybridization of a set of “dots” in the array and an extract of labeled DNA from the sample under study. Usually, the labeled and in some cases fragmented sample DNA is subjected to a denaturation step which separates the DNA duplexes, for example by heat. When the temperature is low, DNA tends to hybridize to its most exact complementary strand due to its physicochemical characteristics. When the DNA comes into contact with the array, if there is a match between the DNA in the sample and the DNA fragment contained in the dot, the labeled DNA copy of the sample binds specifically to the dot. There is a high possibility of remaining. This occurs because the number of complementary DNA copies contained in the array dots is large. In the hybridized array image acquisition and processing stage, this label allows detection of microorganisms present in the sample under investigation.

  DNA labeling can be done by any known labeling technique, the most common being fluorescent or radioactive labels.

  Arrays and their uses are known, and in the state of the art we find examples of arrays that detect the presence of microorganisms in a sample, but genes that code for proteins with biotechnology-related activity There is nothing focused on the detection of.

  There are currently various published protocols for producing arrays of DNA fragments, and there are factories that service the production of this type of array. Manufacturing depends on the protocol used by the company commissioned to manufacture the array or the manufacturing site where it is performed, the carrier, the method of binding the DNA fragments to the carrier, the spatial distribution of the dots on the carrier. As a result, only gene selection and design of the DNA fragments used define the specificity and utility of the array (Ye et al JouRNAl of Microbiological Methods 47 (2001) 257- 272).

Summary of the Invention

  The present invention relates to an array for rapidly and simultaneously identifying the presence of a gene encoding a protein having an activity related to biotechnology present in a microbiological sample, and a method of using the array in the identification of the gene Is disclosed.

Can identify the presence of genes encoding proteins involved in biofilm formation, CO ₂ fixation, energy metabolism, chemotaxis and motility, iron oxidation, nitrogen metabolism, sulfur assimilation, and oxidation-reduction of sulfide compounds A DNA fragment with a nitrogenated base length of less than 100 was designed. All designed fragments are specific for the gene encoding the particular protein associated with the function of interest, and therefore they can be said to be specific for certain metabolic functions. In some cases, the gene on which the oligonucleotide is designed is specific to only one species, and as a result, the oligonucleotide is specific to both the function and the species. In other cases, it is found that the gene of interest is conserved orthologically. In those cases, the oligonucleotides are designed in a consensus region between the described orthologues and are thus specific to the function and the taxon discussed.

If there is at least one of those DNA fragments in the array, it is possible to assess the metabolic characteristics of the microbiological community. Preferably, the presence of some or all of the genes encoding proteins related to biofilm formation, CO ₂ fixation, energy metabolism, chemotaxis, nitrogen metabolism, sulfur assimilation and oxidation-reduction of sulfide compounds, simultaneously and uniquely An array is provided that includes some of the designed DNA fragments that allow for identification in one process.

  The method is provided as a useful tool in biotechnology whenever an assessment or control of the microbiological community characteristics regarding its specific metabolic function is required.

Detailed Description of the Invention

  The provision of an array enabling the rapid and simultaneous detection of the presence of a gene encoding a protein having a biotechnology-related activity present in a microbiological sample, and a method of using the array Will be applied in various fields. For example, minerals for biomining processes; contaminated soil or water for bioremediation processes, or wild microorganisms present in the base of biotechnological processes such as any other biotechnological application Is an academic community evaluation. The second aspect is that it makes it possible to control microbiological communities that are already involved in biotechnological processes.

In order to meet the demands of their biotechnology industry, we designed DNA fragments with less than 100 hydrogenated bases, which are biofilm formation, CO ₂ fixation, energy metabolism, chemotaxis and motility It is specific for genes encoding proteins involved in sex, iron oxidation, nitrogen metabolism, sulfur assimilation, and oxidation-reduction of sulfide compounds. These fragments are distributed in the DNA array to identify the presence of those genes in samples obtained from the microbiological community, whether wild or already involved in biotechnological processes. enable.

All designed fragments are specific for genes that code for specific proteins associated with the function of interest, and therefore they are specific for certain metabolic functions specific for the following functions: Say: biofilm formation, CO ₂ fixation, energy metabolism, chemotaxis and motility, iron oxidation, nitrogen metabolism, sulfur assimilation, and redox of sulfide compounds.

  If the gene on which the oligonucleotide is designed is specific for only one species, then the oligonucleotide is specific for both the function and the species. In other cases, the gene of interest is orthologically conserved in different species of a specific genus. In those cases, the oligonucleotides are designed in a consensus region between the described orthologues and are thus specific to the function and the taxon discussed.

To help understand how we developed this method, we briefly describe the strategy used.
• First, we established the functions of interest in biotechnology that we would like to detect and the key genes for each of those functions.
Second, we selected a microorganism with a gene associated with the function of interest, subject to two basic conditions: one possessing any function of interest, and another complete Or have been partially sequenced.
Third, a list of reference sequences that code for a protein or function of interest in a well-known microorganism such as E. coli is created and the homology between the known gene and the genome of interest is examined. If homology is found, it is defined as a “homologous” zone that matches the gene of interest in the microorganism of interest.

  A start-up database containing each sequence of the gene of interest in each of the microorganisms of interest was obtained with this method. It is clear that not all of the genes of interest are present in all of the microorganisms of interest (this is a known microorganism / sequenced genome).

  Select only the region that is specific to the gene of interest, in other words, select only the region that does not show homology with genes with other functions, and select the sequence of the gene contained in the startup database. Compared with all genes of the microorganism of interest. This selection is made by a search algorithm with those restrictions.

Specific regions chosen in this way are grouped by high similarity, understood as a match of about 80% of the final length of the designed oligonucleotide for the purpose of detecting possible consensus regions To be
These specific regions are identified as useful regions for detection taking into account the physicochemical variables of the oligonucleotide (GC composition level, hybridization temperature, possible secondary structure). Regions subject to these conditions are considered to be oligonucleotide candidates.

  The oligonucleotide candidate is compared to all of the known gene sequences by homology, but this comparison excludes the gene of interest for which the oligonucleotide was designed based on which it is clearly homologous.

  The oligonucleotides used as being specific are what we use to detect the function of interest.

As indicated above, the first step is to select the function of interest. We are present in the microbiological community and are useful for the biotechnology contained in our DNA array: eight metabolic functions: biofilm formation, CO ₂ fixation, energy metabolism, chemotaxis and motility, Iron oxidation, nitrogen metabolism, sulfur assimilation, and oxidation and reduction of sulfide compounds were selected. Their respective importance is apparent to any person who has complete knowledge of the art.

Some of their functions provide us with general and relevant information in any microbiological community, such as both energy metabolism and whether the community can fix CO ₂ or nitrogen Some are related to establishing the nutrition required by the community.

  Knowing the presence of the community's biofilm-forming ability or migratory microorganisms within the community is equally essential to establish the culture conditions for the biotechnological processes we wish to perform . In other respects, iron oxidation, sulfur assimilation, and oxidation / reduction processes of sulfide compounds are important processes in very important and specific biotechnological processes such as biomining and bioremediation. .

  Secondly, the microorganism of interest is determined on which the oligonucleotides contained in the microarray are designed. The selection was carried out while maintaining the two relevant aspects intended: the first being that the microorganism has at least one function of interest, and the second being the sequence of all or part of its genome. That has been decided. Considering those factors, it is of the nature of “Biosigma: Wenelen (DSM 16786)” and “Licanantay (DSM 17318)”, has one or more of the above metabolic functions, and the genome is sequenced A stock is selected. Also included was Acidithiobacillus ferrooxidans ATCC 23270, which has the ability to oxidize iron and sulfide compounds. Two publicly available Burkholderia pseudomallei species are used: Burkholderia pseudomallei 1710b and Burkholderia pseudomallei K96243. Desulfovibrio desulfuricans is selected for sulfur oxidation activity and its published Desulfovibrio desulfuricans G20 species is used; Thiobacillus denitrificans ATCC 25259, whose function is the oxidation of sulfide compounds Was also included. Also included were the biomining microorganisms Ferroplasma, Leptospirillum, Sulfolobus and Thermoplasma, whose published sequences correspond to the following microorganisms; Ferroplasma acidalmanus ( Ferroplasma acidarmanus), Ferroplasma sp. (II), Leptospirillum sp. (II), Leptospirillum sp. (III), Sulfolobus acidocaldarius ) DSM 639, Sulfolobus solfataricus, Sulfolobus tokodaii, Thermoplasma acidophilum and Thermoplasma volcanium.

  Once the function of interest and the microorganism being studied are known, the gene used to identify the presence of the function is determined. A brief description of each of the functions of interest and the genes selected to represent them is provided below.

A description of the function of interest.
Iron oxidation. Iron oxidation from Fe ⁺² to Fe ⁺³ is the iron (III) produced is a very oxidant, which can oxidize sulfides present or any compound that requires oxidation This is necessary for the development of bioleaching in order to be able to release the metal of interest in the solution.

In order to identify the presence of the “iron oxidation” function, the following genes encoding proteins involved in the process were selected based on A. ferrooxidan:
rus, cyc1, cyc2, cycA y cycA2
Since the sequence definitions of all those genes within the microorganism of interest (known microorganism / sequenced genome) are available, it is not necessary to use a reference sequence.

  Biofilm formation. The ability to form a biofilm or silt is important for the development of any microbial community in order to ensure the durability of the biomass bound to the support.

To identify the presence of the “biofilm formation” function, the following genes were selected that code for proteins related within the process.
galE, galU, rfbA, rfbB, rfbC, rfbD, epsD, gdh, manB, manC, pgm, uppS, wza, wzc
Since there was no information available about those genes in the microorganism of interest, the study was conducted using reference genes and the genes and the microorganism of interest were determined to determine the “probable” presence of those genes. We searched for homology between genomes. In most cases, the E. coli gene was used as a reference, except for epsD, where the Lactobacillus lactis gene was used as a reference.

CO ₂ fixed. CO ₂ fixation is microbiological to allow the community to investigate whether it is an autotrophic organism that gives the process an economic advantage because it does not need to add carbon substrates to the community. Important in biotechnological processes.

In order to identify the presence of the “CO ₂ -fixed” function, the following genes were selected that code for proteins involved in the process:
cbbL1, cbbL2, cbbM, cbbS1, cbbS2, cbbArchea, ppc, prk
Information on some of those genes in the microorganisms of interest, cbbL1, cbbL2, cbbM, cbbS1 y cbbS2 was available. Others have studied with reference genes and searched for homology between those reference genes and the genome of the microorganism of interest in order to determine the “probable” presence of those genes. For the gene cbb of Archaeas, Methanosarcina acetivorans was used as a reference, for the gene ppc, the gene of E. coli was used as a reference, and for the gene prk, the gene of Synechococcus was used.

  Chemotaxis and motility. Microbial motility is provided by the presence of flagella; in some cases, movement develops as a response to the concentration of metabolites in the medium, known as chemotaxis. Knowing whether the microbiological community has chemotaxis and motility characteristics makes it possible to better establish the culture conditions for the biotechnological processes being developed.

For identification of the presence of “chemotaxis and motility” function, the following genes were selected that encode proteins associated with the process:
flhA, flhB, fliF, fliG, fliM, fliN, motA, motB
Because there was no information available about those genes in the microorganism of interest, the study was conducted using reference gene genes, and the reference genes and the interests of interest were determined to determine the “probable” presence of those genes. We searched for homology between the genomes of certain microorganisms. In all cases, the E. coli gene was used as a reference.

  Nitrogen metabolism. There are two processes that are considered important for the metabolism of nitrogenated compounds. One is nitrogen fixation, whose essential component is the enzyme nitrogenase. The other is the degradation of the nitrogenated compounds involving nitrate reductase.

In order to identify the presence of the “nitrogen metabolism” function, the following genes were selected that encode proteins related within the process:
nifK, narH, nirA
Since no information was available about those genes in the microorganism of interest, the study was performed using reference gene genes, and the genes and the microorganism of interest were determined to determine the “probable” presence of those genes. We searched for homology between the genomes. In the case of narH, the E. coli gene was used as a reference; in the case of nifK, Azotobacter vinelandii was used as a reference; in the case of nirA, the reference was Synechococcus.

  Sulfur assimilation. Sulfur assimilation is a process in which environmental sulfur is fixed as organic sulfur for use in cell metabolism. The two main end products of this process are the essential amino acids cysteine and methionine.

In order to identify the presence of the “sulfur assimilation” function, the following genes were selected that encode proteins related within the process:
cysI, cysJ
Since there was no information available about those genes in the microorganism of interest, the study was carried out using those reference genes and said genes and said interest of interest to determine the “probable” presence of those genes The homology between microbial genomes was explored. For the genes cysI and cysJ, the E. coli gene was used as a reference.

  Oxidation / reduction of sulfide compounds. A process that is highly relevant to bioleaching is the oxidation of sulfur compounds. For example, microorganisms of the genus Acidithiobacillus can catalyze the oxidation of reduced sulfur compounds (such as sulfides, elemental sulfur, thionates, etc.) using oxygen as an electron acceptor and It is produced as an end product and species such as sulfite and thiosulfate are reduced to intermediate products that solubilize metals associated with sulfides that may be present in the ore.

In order to identify the presence of the “sulfur oxidation” function, the following genes were selected that encode proteins related within the process:
doxA, doxD, doxDA1, doxDA2, dsrA, dsrB, dsrC, dsrE, dsrF, dsrK, dsrL, dsrM, dsrN, dsrO, dsrP, dsrS, soxB
Information was only available on the doxDA1 and doxDA2 genes in the microorganisms of interest. Others have studied with reference genes and searched for homology between the reference genes and the genome of the microorganism of interest in order to determine the “probable” presence of those genes.

For the genes doxA and doxD, the Acidamanus Ambivalens is used as a reference, for the gene soxB the Paracoccus denitrificans gene is used as a reference, and for all dsr genes, allochromati The gene for Allochromatium vinosum was used.

Energy Metabolism To identify the presence of the “energy metabolism” function, the following genes were selected that code for the proteins involved in the process:
pfk, korA, korB, idh, pdhA, pdhB, pdhC, gltA, accA, accB, aaC, accD
Only information about the pdhC gene in the microorganism of interest was available. Others have studied with reference genes and searched for homology between the reference genes and the genome of the microorganism of interest in order to determine the “probable” presence of those genes. For genes pfk, idh, gltA, accA, accB, aaC and accD, E. coli genes were used as references. For the genes korA and korB, Methanocaldococcus jannaschii was used as a reference, and for the genes pdhA and pdhB, the gene of Mycobacterium tuberculosis was used.

The disclosed array of nucleotide sequences for detecting and identifying a gene encoding a protein as defined above having a biotechnology-related activity present in a microbiological sample is one of the following DNA fragments: Contains some or all representatives attached to the surface:
a. At least one DNA fragment that specifically identifies a gene encoding a protein associated with biofilm formation;
b. At least one DNA fragment that specifically identifies a gene encoding a protein associated with CO ₂ fixation;
c. At least one DNA fragment that specifically identifies a gene encoding a protein associated with energy metabolism;
d. At least one DNA fragment that specifically identifies a gene encoding a protein associated with chemotaxis and motility;
e. At least one DNA fragment that specifically identifies a gene encoding a protein associated with iron oxidation;
f. At least one DNA fragment that specifically identifies a gene encoding a protein associated with nitrogen metabolism;
g. At least one DNA fragment that specifically identifies a gene encoding a protein associated with sulfur assimilation;
h. At least one DNA fragment that specifically identifies a gene encoding a protein associated with oxidation / reduction of sulfide compounds;
Here, hundreds of copies of each DNA fragment exist, form dots of a uniform composition, and are spatially distributed on the surface of the carrier.

  For each of these genes, we designed 80 to 100 specific nitrogenated bases, preferably 100 specific nitrogenated base fragments. They are listed in the sequence listing.

  Overall, we have designed a total of 232 DNA fragments for the identification of genes encoding proteins with biotechnology-related activity, all of which are 100 nucleotides long. The sequences of the designed 232 DNA fragments are included in the sequence listing.

SEQ ID NOs: 1-86 outside of the designed 232 sequences are specific for biofilm forming functions.
Sequence 87-101 is, CO ₂ - is specific to a fixed function.
Sequences 102-156 are specific for energy metabolism function.
Sequences 157-192 are specific for chemotaxis and motility functions.
SEQ ID NOs: 193-197 are specific for iron oxidation function.
SEQ ID NOs: 198-203 are specific for nitrogen metabolism function.
SEQ ID NOs 204-210 are specific for sulfur assimilation function.
The sequences 211 to 232 are specific to the redox function of the sulfide compound.

  The designed fragment is complete or is a larger fragment that contains it, or is partial, such as any sub-fragment that contains the fragment, or the reverse of all the above options It can be imprinted into the array as a complementary sequence. A subfragment of 50-70 nucleotides is conveniently imprinted.

  Arrays included in the present invention are completely contained; just like a PCR product, the larger region in which the fragment represents more than 20% of the region; or, in part, in each of the fragments disclosed herein It should be noted that at least one of the DNA fragments contained in SEQ ID NOs: 1-232 is included, either as one of the subfragments to be produced, or as all reverse complement sequences of the above options.

  The above aspects are very important because the specificity of the nucleotide sequence is the same as that of its reverse complement. This feature, specificity, is the most difficult to achieve DNA fragment design. Despite the knowledge of the technology, a set of tools that exist in the technology can identify thermodynamically stable and unstable oligonucleotides, but the stability of complementary sequences It may happen that it is not suitable for. The reverse complementary sequences of SEQ ID NOs: 1-232 of the fragments of the present invention are all complete or are larger fragments containing it, such as PCR products; or partial, each of the fragments disclosed herein Regardless of whether it is one of the sub-fragments containing, it is considered within the scope of the present invention.

  The array preferentially includes at least one fragment or a sub-fragment of each fragment of interest. An array containing all of the disclosed DNA fragments or subfragments can be prepared, or in some cases, an array containing only one of them can be prepared. All of these options, as well as all possible intermediate combinations, are included within the scope of the present invention.

  The effectiveness of the arrays of the present invention is given by the specificity and stability of the imprinted fragments. Those features persist in each of the subfragments contained in the designed fragment. This means that the specificity remains the same even when nucleotides 1-100, or 42-92 or 15-65 or any other possible selection is used. All selections correspond to subfragments and are within the scope of the present invention.

  It is also possible to obtain DNA fragments comprising the fragments or subfragments of the invention flanked by other oligonucleotides through synthesis or as the product of PCR. Larger regions containing the fragments of the present invention, whose specificity is given by the fragments or subfragments designed as disclosed in the present invention, are also found within the scope of the present invention.

  To implement an array according to the present invention, each selected fragment or subfragment is synthesized in hundreds of copies and applied to an array such as glass, silicon, nylon, or any others present in the art Must be imprinted on the carrier to be imprinted as a homogeneous dot.

  What is shown when discussing background information for the present invention is that DNA fragment synthesis and array fabrication techniques are known, and any of them can be used to create the arrays of the present invention. That is all.

  In addition to those DNA fragments specific for genes encoding proteins with biotechnology related activity, each array advantageously includes a negative control and a positive control. Negative controls correspond to nucleotide sequences that are never found in a biotechnological context. Positive controls correspond to nucleotide sequences that are always present in the sample in question.

  Those skilled in the art will recognize that the 232 DNA fragments designed for the present invention are not limited to using arrays, but also other molecular biologicals based on hybridization of unknown DNA to specific fragments. It is clear that the technology also allows the identification of genes that code for proteins with activities related to biotechnology. These molecular biological techniques can include, for example, detection probes, FISH, PCR and sequencing.

  In any molecular biology technique, completely; just like a PCR product, the larger region where the fragment represents more than 20% of the region; or, in part, each of the fragments disclosed herein Using one of the 232 DNA fragments known in the present invention, either as one of the subfragments included; or as all the reverse complement sequences of the above options, is an obvious application of the present disclosure. And are considered to be within the scope of the present invention.

Use of the Array To detect and identify the presence of a gene that encodes a protein with biotechnology-related activity present in a microbiological sample, the array of the present invention is primarily of interest to be evaluated. It is necessary to isolate a sample of DNA. This also allows studies using cDNA, in which case the only difference is that the RNA of the sample is isolated in the first step. In the art, different methods are known for extracting DNA and RNA from environmental samples or from cultures, and any of those methods can be used taking into account each case of the particular nature of the sample.

During the second step, all DNA or RNA of the sample needs to be converted into short fragment labeled DNA that is applied to hybridize with fragments imprinted on the array dots. . If the sample DNA has already been isolated, it needs to be fragmented and labeled. If the sample RNA is not used for research, there is no need for fragmentation, and the production step is performed only to obtain labeled cDNA. The technique allows both DNA fragmentation and production to be made using 6 nucleotide aleatory splitters of DNA. Marking can be performed using nucleotides that are labeled or sensitive to being labeled by any of the techniques existing in the art such as radioactivity, biotin, fluorescence, and the like. If a macroarray is used, the marking is preferably done with radioactivity ³² P, and if a microarray is used, it is done with fluorescence, eg, Cy5 or Cy3.

  The present invention is not limited to the methods described for the preparation of DNA or cDNA for arrays, and any method present in the state of the art for the preparation of DNA or cDNA can be used. Is not meant to be separable from the scope of the invention.

  Once the DNA is prepared, it is subjected to a DNA denaturation step. This denatured DNA is then placed in an amount of DNA mixture on the array and incubated on the array. The array is hybridized at a temperature in the range of 40-70 ° C. for at least 1 hour and preferably overnight.

  After hybridization, the array is carefully washed. Washing is usually performed with a softening solution at a moderate temperature, for example 35-50 ° C, preferably 40-45 ° C.

  Once the array is washed, it is preferentially dried and conveniently, for example, in a falcon tube or something similar, for a short time, at moderate speed (200-3000 xg per 1-5 minutes) Dry by centrifugation.

  Finally, it is necessary to visualize the markings indicated by the dots. The location of each labeled dot indicates the presence of a gene encoding a protein having activity related to biotechnology, on which the DNA fragment is designed.

  It is necessary to check that the dot corresponding to the negative control remains unlabeled. Because if it had hybridized with their DNA fragments, it indicated that the reaction was non-specific, and because they could contain false positives, the results obtained were This is to show that it must be abandoned.

  Dots corresponding to positive controls are similarly labeled. This is because if no hybridization with this DNA fragment occurred, it indicates that there was interference during the reaction, and therefore a dot without signal could represent a false negative. Indicated.

  In accordance with the above, the determination of the presence of a gene encoding a protein with activity related to biotechnology in the microbiological community translates into the reading of labeled dots in the arrays of the invention.

  Example 1: A microarray for detecting and identifying the presence of a gene encoding a protein having an activity related to biotechnology.

Microarrays with 23 different DNA fragments of the present invention were prepared that identify genes encoding proteins with activity related to biotechnology. Their activities are related to the following functions: sulfur assimilation, CO ₂ fixation, iron oxidation, nitrogen metabolism, energy metabolism, chemotaxis and motility, and biofilm formation.

Two positive controls and two negative controls were also included in the microarray. The contents of each position in the microarray are detailed in Table 1 below.

All imprinted fragments were 60 nucleotides long. The selected DNA fragment of the present invention corresponds to a sub-fragment of 60 oligonucleotides of the fragment shown in Table 2 defined in the sequence listing.

  Microarray preparation was commissioned to a company specializing in the service.

  Example 2: Use of a microarrangemt to detect and identify a gene encoding a protein having an activity related to biotechnology.

  The microarray obtained in Example 1 is used to determine the composition of the microbiological community in two samples of bioleaching heap effluents, Sample 1 (S1) and Sample 2 (S2) It was.

Total DNA present in S1 and S2 was extracted by traditional DNA extraction methods.
2 μL of DNA was taken from the sample and placed in an Eppendorf tube. The following method was performed in each case:
36 μL of ddH ₂ O and 3, 3 mL of commercially available aleatory splitters 6 nucleotides (hexamers) were added. Boiled for 5 minutes and then worked in ice.

  2 μL of the nucleotide mixture (where the nucleotide dUTP is labeled with Fluorphor Cy) was added. Cy5 showing red fluorescence was used for S1, while Cy3 showing green fluorescence was used for S2. After this, 4 μL of Klenow-type polymerase and 5 μL of a buffer solution suitable for the polymerase were added (according to the supplier); it was incubated at 37 ° C. for 4 hours.

  The reaction was stopped with 5 μL of AEDT 0,5 M pH 8. Labeled DNA was recovered using an ion exchange column. The effluent containing DNA was dried under reduced pressure.

  Further, 100 μL of Tris buffer solution pH 7 was added as a basic component to resuspend the DNA, and subjected to DNA denaturation at 100 ° C. for 1 minute and a half. Hybridization was performed on a microarray while moving overnight at 55 ° C.

Next morning, 2 × SSC, 0.1% SDS (sodium dodecyl sulfate), twice at 45 ° C .; 0.2 × SSC 0,1% SDS, once at 42 ° C., and 0.2 × SSC, once at 42 ° C. Washed. (Adjusted to 500 SSC 20 ×, 87.6 g NaCl, 50 g sodium citrate (2H ₂ O) and pH 7.0).

  Each microarray was placed in a box with MilliQ water for 15 minutes and then dried in a falcon tube or similar at 1.100 rpm for 1 minute.

  Finally, observation of the microarray results became possible, as shown in FIG. 1 for S1 and FIG. 2 for S2.

In Table 3, the location of the different fragments in the microarray is shown and the results of the hybridization with the DNA obtained from S1, shown in F1, are summarized. ? ? All positive controls showed hybridization and negative controls remained unlabeled.

This result indicates the function of sulfur assimilation, CO ₂ -fixation, iron oxidation, and nitrogen fixation, and the presence of genes encoding proteins related to key proteins in energy metabolism. The sample did not appear to have the genes examined for chemotaxis and biofilm formation.

Table 4 again shows the location of the different fragments on the microarray. The results of hybridization with DNA obtained from S2 shown in F2 are summarized. All positive controls showed hybridization and negative controls remained unlabeled.

  The results indicate the presence of a gene that encodes a protein associated with all the functions investigated. Clearly this is the case for mixed cultures. Unlike the above samples, high cellular presentation was observed for cellular motility and chemotaxis and for biofilm formation.

  The examples are illustrative of the features of the invention and are not to be considered as limiting the scope of the invention as defined by the claims.

[Explanation of drawings]
FIG. 1 corresponds to the result of hybridization between a biomining sample 1 and the microarray of the present invention. The fragments of the present invention correspond to the subfragments of the fragments listed in Table 2. Table 1 shows the contents of each position of the microarray. Table 3 summarizes the positive or negative results of hybridization.

The results indicate the presence of genes that code for proteins associated with the following functions: sulfide metabolism, CO ₂ fixation, iron oxidation, nitrogen fixation, and enzymes key to energy metabolism. The sample does not appear to have genes that are evaluated for chemotaxis and biofilm formation. The positive control showed a hybridization signal and the negative control remained unlabeled.

  FIG. 2 corresponds to the result of hybridization between the second biomining sample and the microarray of the present invention. The microarray used is the same as that used for sample 1. The contents are shown in Table 1. Fragments used in this example are included in the sequences listed in Table 2, and Table 4 summarizes the positive or negative results of hybridization.

  The results indicate the presence of genes encoding proteins associated with all assessed functions, which is likely the case with mixed cultures. Unlike the above samples, cellular motility and chemotaxis, and high gene expression for biofilm formation are observed. The positive control showed a hybridization signal and the negative control remained unlabeled.

  [Sequence Listing]
SEQ ID NO: 1
Function: Biofilm formation
Code: BMS92
Gene: epsD
Sequence: GGG AAG TCA TCC AGG TCA TCT CCG TCC TGG CTG TCC TCA ATG CGG CTT TCG CCT TTT CCG GCA GGC TGC CAT TGT CGC GGC TGT GGC TGT TCA GTA CCT G
SEQ ID NO: 2
Function: Biofilm formation
Code: BMS196
Gene: epsD
Sequence: ATG GCC ACC ATA AGA ACC AGT ATG CGC ACA CAC TCT ACC TTC CAA TGG TCT AGC TGG ACG CCT TTT ATT ATG GCG CTG GGA GAT ATT TTA GCC TTT CTT G
SEQ ID NO: 3
Function: Biofilm formation
Code: BMS229
Gene: epsD
Sequence: GGG TGC TTT ATC CCG TCG CCA TGG CGG CAG GTG ATC TGG TTG CGT TCT ATT CGG CGT TGC TGG CAG CGT ATT TCC TCA GGG TCC ATC TTT TGG GGC ACT G
SEQ ID NO: 4
Function: Biofilm formation
Code: BMS10308
Gene: epsD
Sequence: CTC GCC GCG AAG CCC GGC ATG ACG GGG CTT TGG CAA GTG AGC GGG CGC AAC GAT ACC AGC TAC GCG ACA CGG GTG TCG CTC GAC GTG TCG TAC GTG AAG G
SEQ ID NO: 5
Function: Biofilm formation
Code: BMS598
Gene: epsD
Sequence: ATG AAT GAG GGA TGT ACG CTG CAG GGC GGT TGC CGG CTG ATG ACT TCC GGC ATG CGT ATG ACG CTG CTG ATG CTG GCA TCG GAT ATC TTT GCA CTG GTC T
SEQ ID NO: 6
Function: Biofilm formation
Code: BMS678
Gene: epsD
Sequence: GGG GAT TTG TCT GCG TTC TAT CTG GCT CTG TGG ATC TCT TAC ATT TTG AGA GTG GAC CTT CTC AGT CGG TGG ATT CCA GTT CCC TTT ACC CGA AGC TTC G
SEQ ID NO: 7
Function: Biofilm formation
Code: BMS10025
Gene: galE
Sequence: TTA CCC AAC GCC CGA TGG CAC CTG CRT TCG GGA TTA CAT TCA TGT CTG GGA TCT GTG CAG CGC CCA CTT GCT GGC TTT AGA GCA TTT GCT GGC YGA TGG G
SEQ ID NO: 8
Function: Biofilm formation
Code: BMS607
Gene: galE
Sequence: CGG CAT AGG TGA GGC GCA TTC GCC CGA AAC CCA TCT GCT GCC CAA CGT ACT GCT GGC GGC ATT GGG CAG AAA CGA CGG CCT GAA GAT ATT CGG CGA TGA T
SEQ ID NO: 9
Function: Biofilm formation
Code: BMS10080
Gene: galE
Sequence: GAT CGA GGC GGT CAT CCA TTT TGC GGC CGC GAT CGA AGT CGG GGA ATC CGT CCA GGA TCC YCT CAA ATA CTG GGA CAA CAA CCT GAA CGG CAC GCT CCG G
SEQ ID NO: 10
Function: Biofilm formation
Code: BMS673
Gene: galE
Sequence: GGT GCG GAT CGA GCT ACA GCA TCC TCG ACC TCG TCA AGG CCC TCT CTG AGA TCC TGA AGG TCA CGC CGG AGA TCC GCC ACA ACC CTC CCC GGG CCG GGG A
SEQ ID NO: 11
Function: Biofilm formation
Code: BMS920
Gene: galE
Sequence: ACG ATG GCC GAG GTC GGG CTG AAG ACA ATC GTG TTC TCG TCG TCG GCG ACG GTC TAC GGC GAC CCG GTC CGG GTG CCG ATC ACC GAA GAT TTT CCG CTG T
SEQ ID NO: 12
Function: Biofilm formation
Code: BMS65
Gene: galU
Sequence: TGA TGG CCC GTC CGG TGG TGG GTG ACG AAC CCT TTG CGG TCT TGC TGG CGG ACG ATC TTA TGC TGA GCG AGT CTC CTG TGC TTG CGC AAA TGG TGG AAC A
SEQ ID NO: 13
Function: Biofilm formation
Code: BMS155
Gene: galU
Sequence: GGG GTA AAC GTG CTA TTG CCG ACC ATT TTG ATG TCG CCT ATG AGC TGG AAA CAG AAC TTG AAA AGC GCG GTA AAA CAG CTC TAC TGA AGG AAA TTC AGA A
SEQ ID NO: 14
Function: Biofilm formation
Code: BMS247
Gene: galU
Sequence: TTG TCG ACG GAC CCG CAC CGG CCC TGC GCC AGC TGG TGG AGG TGT TCA GAA AAG TCA AGG GAC CTG TGA TTG GAG TCC AGA AAG TTC CGC AGC AGG ATG T
SEQ ID NO: 15
Function: Biofilm formation
Code: BMS540
Gene: galU
Sequence: CCC CGG TAT TTT CAG AGT GCA CGA TCT TGT GGA AAA GCC CGC CGT CAG CGA AGC TCC GTC GCG TCT TGC CAT TGT GGG ACG TTA CAT TCT GAC TCC TGA T
SEQ ID NO: 16
Function: Biofilm formation
Code: BMS717
Gene: galU
Sequence: GGG GAA GCG CGC GAT CGA GGA TCA CTT TGA TAT CTC CTA CGA ACT CGA AGA CGT CCT CCG TCA GAA AGG CAA GAT GGC CCT CCT GGA AGA GGT CCG CAA G
SEQ ID NO: 17
Function: Biofilm formation
Code: BMS703
Gene: galU
Sequence: CGC TTT GCC CTC AAG CGG CCG GAT CTT GCG GAG GAG TTC CGG CAC TTC CTG GCC GCG ACC CTC GGG AAG GAG GGC TCT TCG TCC CTG GGA GAC GGA CAC G
SEQ ID NO: 18
Function: Biofilm formation
Code: BMS1012
Gene: galU
Sequence: GAC GGC GCG ACG TCG GTC ATG AAG CAG ATG GTC GAC CAG TAC AGC TAT TAC CAG TGC TCG GTC CTC GGC GTA CAG CAG GTC GCG CCG GAA GAC ACG GCA T
SEQ ID NO: 19
Function: Biofilm formation
Code: BMS10249
Gene: gdh
Sequence: TAG CCG ACG CCG TGG GCC GAC ATC TRC AGC GCC CGG CYA TCG TCA TCA ACA AAT CCA CCG TYC CAG TGG GCA CGG CGC AAA AAG TGC GGG AAC GCA TCG C
SEQ ID NO: 20
Function: Biofilm formation
Code: BMS171
Gene: gdh
Sequence: ATG AAA GTA ACG GTT GTC GGA ACC GGA TAT GTG GGT CTG GTC ACT GGA GCC TGC CTT GCC CAG GTC GGT AAT CAG GTG CTT TGC GTG GAT ATT GAT GCG G
SEQ ID NO: 21
Function: Biofilm formation
Code: BMS10146
Gene: gdh
Sequence: GCT CGG CAT CGA GTA CTA CCC GAT CGG GCG GCC CGG CGC GGC GCC GCG CAC GGC GGC GGG CGA GTC CGA CGC CGC TCG CGC CGC GCG CGC CGG CGC GTG A
SEQ ID NO: 22
Function: Biofilm formation
Code: BMS584
Gene: gdh
Sequence: ATG AAC GTT TGT ATT GTC GGT ACT GGC TAT GTA GGA CTT GTC AGC GCC GCC TGC TTT GCT GAA ATG GGT AAT ACC GTC CGT TGC GTG GAC GTG AAT CCC G
SEQ ID NO: 23
Function: Biofilm formation
Code: BMS766
Gene: gdh
Sequence: TAG ATT TCT CTT CAC CAC AGA TTA CTC CGC GTT AAA AGA CGT GGA TAT TGT CTT TAT TAC AGT CGC TAC ACC TAC CGT AGA AGG GAA GAA CTA TAT AGG C
SEQ ID NO: 24
Function: Biofilm formation
Code: BMS1077
Gene: gdh
Sequence: GCG TTT CCG CCT ACG ACC CGG CGG CGA TGG ACG AGA CCC GCC GTA TCT ACG GCG AGC GGG CTG ACC TGC AAC TCG TCG ACA GCC CGA TGG ACG CCT TGA A
SEQ ID NO: 25
Function: Biofilm formation
Code: BMS322
Gene: manB
Sequence: GGG ATC TCT ACA GCT CCC TGG GAA TTG ACG CCA AAT TTC TGT ACG AGG AAC CGG ACG GAC ATT TTC CCC ACC ACC ACC CGG ACC CGA CCG TTC CCG AAA A
SEQ ID NO: 26
Function: Biofilm formation
Code: BMS591
Gene: manB
Sequence: TAA AAT TTT TTG ATA AAG ACG GAT TCA AGC TGC CGG ATT CGC AGG AAG ATG CCA TAA CTG AAA TGG TTC TGA ACC AGA ACC ACC GCT GGG ACT ACC CGG A
SEQ ID NO: 27
Function: Biofilm formation
Code: BMS656
Gene: manB
Sequence: GAA TCG GTT AAT AGT GAA GCC ATC AGA GAA AGG CAT TTG AAT GTG GCA ATT GAT ACA GGA AAT GGT GCA TCA TAT TAC ACA AGC CCG GCA ATA TTG TCA G
SEQ ID NO: 28
Function: Biofilm formation
Code: BMS798
Gene: manB
Sequence: GTT AAA AAT CCT AAA GCT ATA AAG AAG ATT GTT ACT GCT GTT TCA AGC TCA AGC TTA GTA GAA GAG TAT TTA AGT AAA TAT AAT ATT CAA GTT GAT TGG A
SEQ ID NO: 29
Function: Biofilm formation
Code: BMS827
Gene: manB
Sequence: GGG TTG GGA AGA GCA TAG GCA TGT TCG TAT CTG GCG ACG TTG CCG TCG GAA GGG ATA CAC GGA TCA GCG GCG ATA TGA TAG CCT CTT CCG TTC TGG CTG G
SEQ ID NO: 30
Function: Biofilm formation
Code: BMS847
Gene: manB
Sequence: GTA CTT GCT GAT GCA GGA AAT GGT GCG GCA TAC TAT TCA ACT CCT AAA TTG CTT GAA AAA CTT GGA TGC TCA ATC ACT ACG TTA AAT GCA AAT CCG GAT G
SEQ ID NO: 31
Function: Biofilm formation
Code: BMS969
Gene: manB
Sequence: GAC CTT CAC GCC TGA AAT CGT CGA GGC CAT CGG TCA TGC CAT CGG CTC GGA AGC CGC CGC CCG CGG CCA GAA GGA AAT CTG CAT CGG CCG CGA CGG CCG T
SEQ ID NO: 32
Function: Biofilm formation
Code: BMS10015
Gene: manC
Sequence: TAT YCA CRC CAC CGT CCA YCG YCC CTG GGG CAC CTA CAC CAC GCT GGA AGA RGG CGA CCR YTT CAA GAT CAA GCG GAT TGT CGT CAA GCC CGG TGA AAA G
SEQ ID NO: 33
Function: Biofilm formation
Code: BMS301
Gene: manC
Sequence: GGG CCG AAG CAT GAA TCG GAA ACC CGC CGC ATT CTT CGT CTC GCG GAA AAG GAA GGT CTT CCG GCG AAG GTA TTT GTC GAA CCC CAG TCC CGG AAC ACC C
SEQ ID NO: 34
Function: Biofilm formation
Code: BMS10130
Gene: manC
Sequence: GGG TAC TAC TGG AAT AGC GGC ATG TTC ATG CTG AAA GCC GCC ACG TAC ATG GAA GAA CTG CAT CGC CAT GCA CCG GAA ATT GCC CGC CAG GCC GAA TTC G
SEQ ID NO: 35
Function: Biofilm formation
Code: BMS535
Gene: manC
Sequence: AGC GTG CCG TGC AGG CAG GGG CGG AAC TGG CCG CAC AGA ACC ACT TTG TGA CGT TCG GCA TAC CTC CGG CAC ATC CGG AAA CTG GTT ACG GAT ACA TTC A
SEQ ID NO: 36
Function: Biofilm formation
Code: BMS927
Gene: manC
Sequence: ACG AGG TTA TGC TCC TGG CCG AAA ACC AGT CGA CCT ACA TAC CGC TCG GCA CCA CCC ACC GGC TCG AGA ACC CCG GCA AGG TCG ATC TTC ACA TGA TCG A
SEQ ID NO: 37
Function: Biofilm formation
Code: BMS227
Gene: pgm
Sequence: TCT TCC TGG GGT TCG ATA CCC ATG CCC TTT CCT GGC CCG CTT TTG TCT CGG CCG TGG AAG TCC TGG CGG CAA ACG ATG TGG ATG TCC GGA TCG CCG AAA A
SEQ ID NO: 38
Function: Biofilm formation
Code: BMS567
Gene: pgm
Sequence: ATG ACT ACT GCC GCC AAA GCC GGC CAG TTG CCT GCT CAC GAC GAG CTT ATA AAC ATA GCA CGG CTT GTA AGC GAC TTT TAC ACC GTC AGC CCG GAC CGC C
SEQ ID NO: 39
Function: Biofilm formation
Code: BMS11
Gene: rfbA
Sequence: CGA CCA CCA GGC CGT CGG TCT CGA AGA AAA ACC GGA CGT TCC GCG CTC GTG CTA CGC GGT AAC CGG GCT GTA TTT CTA TGA CGG AAG TGG CAG TCG GAT G
SEQ ID NO: 40
Function: Biofilm formation
Code: BMS166
Gene: rfbA
Sequence: GGG GCA ACG ATT TTT GCC TAC CAC GTT GCC AAT CCC AAG GCT TAT GGC GTG GTG GAG TTT GAC CGT CAG GGG ATT GCC ATT GGA CTG GAA GAA AAA CCT G
SEQ ID NO: 41
Function: Biofilm formation
Code: BMS253
Gene: rfbA
Sequence: TCT TCG GCC ACG GAC TTC TGG AGA GTC TCC GCC GGG GAA CAA ACC TCC GCA AGG GTG CAC TGA TTT TCG GCT ATC CGG TCC GGG ACC CGG AGC GAT ATG G
SEQ ID NO: 42
Function: Biofilm formation
Code: BMS10265
Gene: rfbA
Sequence: CCG ATC ACG CAT GCG GTG TCG AAG CAG CTG YTG CCG RTY TAC GAC AAG CCG ATG ATC TAT TAC CCG CTG TCG ACG CTG ATG GTG GCG GAC ATT CGC GAT G
SEQ ID NO: 43
Function: Biofilm formation
Code: BMS600
Gene: rfbA
Sequence: CCG GTA TAT GAC AAG CCC ATG ATA TAT TAT CCG CTT TCC GTG CTG ATG CTG GCA GGC ATC CGC GAG GTG TGC ATC ATC TCC ACG CCT GCC GAC CTG CCG C
SEQ ID NO: 44
Function: Biofilm formation
Code: BMS671
Gene: rfbA
Sequence: GGG GTA GCC TGG TTC GAC ACC GGA TTT TTC TGT TTT CCC GTT GTT TGC CCG CGC CCT TTC CTC TCC TTG CTT TTT GAG GAT ATC ATT GTC ATG TTT TCG T
SEQ ID NO: 45
Function: Biofilm formation
Code: BMS744
Gene: rfbA
Sequence: ACA CAC AGG ACC AAA GCA ACT CAT CAA GAT CGC CGG CAA ACC CAT ATC CTT ATG GGG CGT TTT ATC ACT GAG GGA TAT AGG CAT AAG AGA CTT TGG GAT T
SEQ ID NO: 46
Function: Biofilm formation
Code: BMS10237
Gene: rfbA
Sequence: ATG GAG GCA GTA ATC TTA CAC GGA GGA CAA GGA ACT CGT TTA AGA CCA TTA ACT CAY ACT GGY CCT AAR CAR TTA ATA AAA GTT GCA GGA AAG CCA ATA T
SEQ ID NO: 47
Function: Biofilm formation
Code: BMS843
Gene: rfbA
Sequence: CTC TAA TCT ATA TGA AGA TTT TAC AAA TGC TGG ATC GGA TGG CCA TCT AGG TTT AGT TCC AGT TGA TAA TCC TTC CCA ATT CGG TAT AGC TGA GGT CGA C
SEQ ID NO: 48
Function: Biofilm formation
Code: BMS10049
Gene: rfbB
Sequence: ATC ATC GTC AAT ATT CTT CAC AAT AAA GCC CTG CCC ATY TAT GGY GAT GGY CRG CAG ATC CGT GAC TGG CTC TAT GTG GAA GAY CAY TGC CGG GGG ATC G
SEQ ID NO: 49
Function: Biofilm formation
Code: BMS296
Gene: rfbB
Sequence: CTG TCG ATC CTG GAC GCA AGG CGG CCG CGT CCG TCG GGG GCT TCC TAC CGG GAT CTG ATC CGC TTT GTT CCG GAC CGC CCC GGC CAC GAC AGA CGA TAC G
SEQ ID NO: 50
Function: Biofilm formation
Code: BMS10312
Gene: rfbB
Sequence: AAG CCG GCC GAG ACG TTC GAG ACC GGA CTT GCG AAG ACC GTG CGC TGG TAT CTC GAC CAT CAG GAG TGG GTC GAC GAA GTG GTG TCG GGC GAT TAC CGC A
SEQ ID NO: 51
Function: Biofilm formation
Code: BMS617
Gene: rfbB
Sequence: ATA AAG CGT TTT GTC CAC ATA TCC ACG GAC GAG GTG TAC GGT TCG CTG CTG CCC CAC GAG GCA CCC TTC ACG GAA AGC AAC CCT CTG CTG CCC AAC AGC C
SEQ ID NO: 52
Function: Biofilm formation
Code: BMS682
Gene: rfbB
Sequence: TCC GGG GGA GAC CTA CGC CAT TGG CGC GGG GAA CCC CCG CAC CAA TAA AGA GCT GGT CCT GAC CCT CTG CGA GAT CCT CGA CCG GCT GGC TCC ACG TCC C
SEQ ID NO: 53
Function: Biofilm formation
Code: BMS729
Gene: rfbB
Sequence: TGG AGT AGT AAA CTT GTT AGA AAT ATG CAG AAG GTA TGA TAC CCG CCT AGT TCA AAT CTC AAC TGA CGA AGT TTA CGG AGA ACA GGA GAA TGC TAC AGA G
SEQ ID NO: 54
Function: Biofilm formation
Code: BMS10334
Gene: rfbB
Sequence: GGA AAT TCA ATT TCA AAT ACG TCC ACA TCT CTA CAG ATG AGG TTT AYG GTG AGG AGT GYG GGG ATG AGR AYT CAC CTT TAA AAC CCT CTT CTC CTT ATA G
SEQ ID NO: 55
Function: Biofilm formation
Code: BMS862
Gene: rfbB
Sequence: CAA TTT TAG AAT ACC TAT TTA CGG AAG TGG AAG GCA AAT TAG AGA CTG GAT ACA TGT ACT GGA TCA CTG TAG TGC GAT CGA AGC TAT ACT GGA AAG AGG C
SEQ ID NO: 56
Function: Biofilm formation
Code: BMS1094
Gene: rfbB
Sequence: ACA CCT ACG GGC TGC CGG TGC TGA CGA CCA ACT GCT CCA ACA ACT ACG GTC CCT ACC AAT TCC CCG AAA AAC TCA TTC CGC TGA TCG TGC TCA ATG CCG T
SEQ ID NO: 57
Function: Biofilm formation
Code: BMS30
Gene: rfbC
Sequence: CTC CTG GTT TCG CCC ACG GCT TTT ACG TGC TCA GCG AGT CGG CGG ACT TTC TCT ACA AAA CCA CGG AGT TTT ATG CCC CGG AAG CGG AGC GCT GCG TGA T
SEQ ID NO: 58
Function: Biofilm formation
Code: BMS181
Gene: rfbC
Sequence: CTT TGC GCA TGG CTT TTA TGT ATT AAG TAA TTC CGC TGA CTT CCT CTA TAA AAC AAC CGA ATT TTA CGC CCC GGA AGC CGA GCG CTG CAT AGT CTG GAA T
SEQ ID NO: 59
Function: Biofilm formation
Code: BMS250
Gene: rfbC
Sequence: CAC ATT CGG CCA GTG GTT CGG ACT CGT TCT CAC GGC CCG TGA TCC GGA GTT CCT GTA TGT TCC GGA GGG ATT GGC CCA CGG CTT TCT GTC GCT CGA AGA A
SEQ ID NO: 60
Function: Biofilm formation
Code: BMS470
Gene: rfbC
Sequence: GGG CAT CGA ATT CGT TCA GGA CAA TCA CTC CCG ATC CGC CCG TGG CGT GCT TCG CGG CCT GCA CTA TCA AAT TCA GCA CGC GCA AGG GAA ACT CGT GCG T
SEQ ID NO: 61
Function: Biofilm formation
Code: BMS531
Gene: rfbC
Sequence: GTA AAG CCT ACA CAG CGG TGC TCA GTG CCG CAA ACT TTC TGC GCC TGT TCA TTC CCC GGG GCT TCG CGC ACG GCT ACA TGA CAC TGG AAC CCC ACA CCG A
SEQ ID NO: 62
Function: Biofilm formation
Code: BMS837
Gene: rfbC
Sequence: TAT TGT GTG CAA TAG ATA TGA AGA TAA CAG AGG GTA TTT TGA AGA GCG TTA CAA AGC TTC AGA ATT TGA TCG AAT ACT ACA TGT TAG ATT TGT TCA GGA T
SEQ ID NO: 63
Function: Biofilm formation
Code: BMS980
Gene: rfbC
Sequence: TCG CCG AAT TTC GGC CGC TGG GTG GGT TAC GAG CTG TCG GCC GAA AAT CAG AGA ATG CTG TGG ATT CCG CCC GGC TTC GCC CAT GGC TTT CTG GTG TTG T
SEQ ID NO: 64
Function: Biofilm formation
Code: BMS5
Gene: rfbD
Sequence: ACG GTG AGC GGC AGG TAC AGG CCA TTC TCG GTG ATA CCG CAC TCA TCC TGC GTA CCG CCT GGG TCT ATA GCG CCC ACG GCA GCA ACT TCG TCA AAA CCA T
SEQ ID NO: 65
Function: Biofilm formation
Code: BMS168
Gene: rfbD
Sequence: GGG ATG GTG CCG TTC ATT GTG CAG AGG CTG CCC GTA AAC ATT CTG CCC GGC TCA TCC ATA TTT CCA CGG ATT TTG TAT TTG ATG GGA GTC AGG CGA CCC C
SEQ ID NO: 66
Function: Biofilm formation
Code: BMS10142
Gene: rfbD
Sequence: TGG CGG TTG CGG AGA CCG GCG GCG ACT GGC TGA CGT TTC GCA CCA CGT GGG TGT TCG CCG CAC GCG GCA AGA ACT TCC TGC GAA CGA TGC TTC GTC TTG C
SEQ ID NO: 67
Function: Biofilm formation
Code: BMS622
Gene: rfbD
Sequence: GGC AAC ATG CAT CTT GTC CAC TAC AGC ACC GAC TTC GTC TTT AAC GGA CGC AAG GAA ACG CCG TAT ACA GAA GAT GAC GAA ACC GCC CCG CAG AGC ATC T
SEQ ID NO: 68
Function: Biofilm formation
Code: BMS940
Gene: rfbD
Sequence: GCG CGA GGC AGC AAC TTC CTG CTG ACG ATG ACG CGC CTC ATG CGC GAA CGG CCG GAA CTC AAG ATC GTC GCC GAT CAG ATG GGC GCC CCG ACC TGG TGT C
SEQ ID NO: 69
Function: Biofilm formation
Code: BMS10060
Gene: uppS
Sequence: GGG AGA TGG TGC AGT CCT GCG CGG ATC TCG GTA TTC CCT ACC TGA CGC TTT TCG CTT TCA GCA CCG AGA ACT GGC GAC GTC CAG CCA TTG AAG TTC GTT T
SEQ ID NO: 70
Function: Biofilm formation
Code: BMS186
Gene: uppS
Sequence: GGG AGA TGG TTC AGT CGT GTG TAG ACT TGA ATA TTC CCC ATC TTA CTC TCT TTG CGT TTA GCA CTG AAA ATT GGC GGC GGC CTC CTT TGG AGG TGC GTC T
SEQ ID NO: 71
Function: Biofilm formation
Code: BMS10109
Gene: uppS
Sequence: CGG GCG TCG AAT ATC TGA CGC TCT TCG CGT TCA GCT CCG AGA ACT GGC GCC GGC CGA ACG ACG AAG TGT CGT TCC TGA TGC GCC TGT TCA TCA CCG CGC T
SEQ ID NO: 72
Function: Biofilm formation
Code: BMS595
Gene: uppS
Sequence: GAC TCT TTA CGC GTT TTC GCA GGA AAA CTG GGG ACG GCC CAA AAA AGA AGT GGG GTT CCT CTT TGA TCT GCT GGT CAG CTT TCT GCG TGA TGA ACT GCC C
SEQ ID NO: 73
Function: Biofilm formation
Code: BMS642
Gene: uppS
Sequence: ATG AGT ATA GCC CAT AAG ATA GGA GAT ATC GCC TCA GGC GTT TAC GAA CAG GCT TTA ATG GAA GAG ATA AGG AAA GCC GAT GTC CCA CTT CAT CTA GGG A
SEQ ID NO: 74
Function: Biofilm formation
Code: BMS659
Gene: uppS
Sequence: GGG TTA TCT ACA AAT GAG GGG CAT GTG AGG GGA AAA GAT AAA TTA GAA GAG GTC CTT GAC TGG GCT ATG GAG GTT GGA ATC AAA ATA GTT ACA GTA TAC G
SEQ ID NO: 75
Function: Biofilm formation
Code: BMS10074
Gene: uppS
Sequence: TGG CCT AYA CCG AAA TGT ACT TTA CRC CGA CCY TGT GGC CGG ATT TCA GAG AAG AGG ATT TTC GGC AGG CGA TCG AAG ATT TCC AGG AGA GAA GAC GCC G
SEQ ID NO: 76
Function: Biofilm formation
Code: BMS733
Gene: uppS
Sequence: CGA TTT GTT ATG GAG GCA GGC AAG AAA TAT TGG ATG CTA TAA GGA AAA TCA TGA ATG ATT ATA AAT TAG GTA TAA TAG ATT CAA AAT CCA TAG ATG AGT C
SEQ ID NO: 77
Function: Biofilm formation
Code: BMS775
Gene: uppS
Sequence: GCT ATC AAT AAT AAC AAC ATA TAT TAA GAG AGG TAT ACA AGA TTT ACT AGA AGA TCC CAT CGT AGA TAA GTA TGA AGT TAA AGT AAG TGC AAT AGG TAA A
SEQ ID NO: 78
Function: Biofilm formation
Code: BMS810
Gene: uppS
Sequence: ATA CGA ATA AGT AAT TTC CTT TTA TGG CAT ATA GCG TAT TCA GAA CTC TTT TTT GTA GAC ACA TAT TGG CCA GAC TTT AGA AAG ATA GAC TTA TGG AGA G
SEQ ID NO: 79
Function: Biofilm formation
Code: BMS822
Gene: uppS
Sequence: TTG TTG ACT GGT GCA TGG AAC TAG ACA TAA GAA TCG TAA CGT TTT ATG CAT TCT CGA CAG AGA ACT TTC GTA GGA GTC CCG AAG AGG TCG ATT TCC TCT T
SEQ ID NO: 80
Function: Biofilm formation
Code: BMS857
Gene: uppS
Sequence: TCA ATG CTC CCA GAG TAT CTA AGA GAG ACC ATT AAG ATA ACA GAG GAA ACG ACT AAA AAC TTC TCT AAT TAC CAT ATG AAC CTC GCC ATA GGG TAC GGA G
SEQ ID NO: 81
Function: Biofilm formation
Code: BMS1044
Gene: uppS
Sequence: GAC AAC AGC CGC CTC ACG TTC ACG GTC GCC GCG AAT TAC GGC GGA CGC TGG GAC GTC GTG CAG GCC GTC AAG AAA CTC GTC GCG TCG GGC GAA CCG GTG A
SEQ ID NO: 82
Function: Biofilm formation
Code: BMS2
Gene: wza
Sequence: GGG AGA TAT CGT CTA CGT GCC GCA AAC GAC CGT CAG CAG CTG GAA TCA AGC CAT ACT GCT CCT GTT GCC GTC CCT GCA GGC CAT CAA CCA GTT GCT CAA C
SEQ ID NO: 83
Function: Biofilm formation
Code: BMS208
Gene: wza
Sequence: TTT GGG TGG CAC AGT GAA TCT ACC AGA GGC CGA TTT GCG TGG GGC TTA TAT CGT ACA AAA CGG TAA GAA ATT GCC GGT CAA CTT CCA TCA GCT ACT CAG A
SEQ ID NO: 84
Function: Biofilm formation
Code: BMS10190
Gene: wza
Sequence: CGC GCT CAC GGC GGG AGG CGG CAT TCT CGA TAC CGA TGC GAA TCC GCG TCA GGT GTA CGT GTT GCG CGA TCT GCA GGA CAA GCC GAA CAC ACC GGA CAT C
SEQ ID NO: 85
Function: Biofilm formation
Code: BMS10037
Gene: wzc
Sequence: CAG TAT CAG ATT CTC CAT CAC GGC CAA GCC TTG CTC ACC GGC AAG ATC GAY CAG CCY GCC GTT GGA CCC GAG TTG CGG CTG ATG CTG ACT GCG GTG AAG T
SEQ ID NO: 86
Function: Biofilm formation
Code: BMS209
Gene: wzc
Sequence: GTG ACT GAT CAC ACT GAA AAT ATG CAT CCG TCC GAG AAC GTC GAT CTG CAA CGG CGG CCA TCA ATT GCC AAC AAC TCT GAA CGT ATG GGA GAT GAA GGA G
SEQ ID NO: 87
Function: CO₂fixation
Code: BMS10077
Gene: cbbArchea
Sequence: TGG AGG AAC TGG CCC GCC TTG AAA AGG CGC CGA TCA TGG CCC AYA TGG CCA TGT CCG GYT GGC TCT GGC ACA AGG ACG GGA TGT CGG TGC GTT CCT GGA C
SEQ ID NO: 88
Function: Fixation of CO₂
Code: BMS692
Gene: cbbArchea
Sequence: AGT CCT GGG ACG CCT GGT CCC AGA AGA TCC CTC TTG AAA AAT ACC GAA AGG GCC ACA AGG CTC TGG ATC GGG CAC TCA CAG CCT TTG CTA CCT ATG TGT G
SEQ ID NO: 89
Function: Fixation of CO₂
Code: BMS10344
Gene: cbbL1
Sequence: AAG GCA AGG CTG TTC TCA CTG AGG CGG CCA AGC ACT CCC CCG AAC TCA AGA TYG CCA TGG AAA CGT GGA AAG AGA TCA AGT TCG ART TCG ATA CGG TCG A
SEQ ID NO: 90
Function: Fixation of CO₂
Code: BMS10053
Gene: cbbL2
Sequence: CCC CGA GTA CGT GCC TTT GGA CTC TGA TAT CTT AGC CTG TTT CAA AAT TAC GCC ACA ACC AGG GGT GGA TCG TGA AGA AGC CGC CGC CGC GGT AGC CGC C
SEQ ID NO: 91
Function: Fixation of CO₂
Code: BMS10069
Gene: cbbM
Sequence: ATG GAC CAG TCC TCA CGT TAT GCC GAT CTC TCT CTG CGC GAA GAA GAT CTG ATC GCC GGT GGC AAG CAC ATA CTG GTT GCC TAC AAA ATG AAA CCC AAA G
SEQ ID NO: 92
Function: Fixation of CO₂
Code: BMS10325
Gene: cbbM
Sequence: GGT CAG GCC AAG CTG TTC TCG ATG AAC ATC ACC GCY GAC GAC CAY TAC GAR ATG TGC GCC CGC GCC GAC TAC GCG CTC GAA GTC TTC GGC CCC GAC GCC G
SEQ ID NO: 93
Function: Fixation of CO₂
Code: BMS64
Gene: cbbS1
Sequence: GGC AAC TAC TGG TAT ATG TGG AAG TTG CCC ATG TTC GGC GAA ACG GAC GTG GAC ACC ATT CTG AAA GAA GCG GAA GCC TGC CAT AAG GCG AAT CCC CAT A
SEQ ID NO: 94
Function: Fijacion de CO₂
Code: BMS10030
Gene: cbbS2
Sequence: CGC ATC AAC ATA CTA CTG GTA CAT GTG GAA ACT GCC CAT GTT CGG CGA GCA GTC AGT GGA CAC CGT TAT TGC CGA ACT GGA GGC ATG CCA TCG CGC CCA C
SEQ ID NO: 95
Function: Fixation of CO₂
Code: BMS203
Gene: cbbS2
Sequence: GGG CTC AGA TTC AGT ACA TTG TCG CTC AGG GTT GGA ACC CTG CAG TAG AGC ATG TCG AGC CCA GTC GCT CGT TCA CGC ATT ACT GGT ACA TGT GGA AGC T
SEQ ID NO: 96
Function: Fijacion de CO₂
Code: BMS1037
Gene: cbbS2
Sequence: GCG GAC ATC CGC AAG CAG GTC GAG TAC CTG GTC TCC AAG GGC TGG AAC CCG GCC ATC GAG CAC ACC GAG CCC GAG CAC CTG ATG GAC TCG TAC TGG TAC A
SEQ ID NO: 97
Function: Fixation of CO₂
Code: BMS10006
Gene: ppc
Sequence: GGG ACT YTA CAG YGA RAT YGC CGT YAC CCC RCT GTT TGA RAC CAT TCA CGA TCT GGA GCG TAT GGA TRC TGT CAT GTC CAC GCT GCT CGA TAA CCC GGT T
SEQ ID NO: 98
Function: Fijacion de CO₂
Code: BMS10137
Gene: ppc
Sequence: AGG AAG TGA TGC TCG GCT ATT CGG ACA GCA ACA AGG ACG GCG GCT TCC TCA CGT CGA ACT GGG AGC TCT ATC GCG CGG AAC TGG CGC TCG TCG ATC TGT T
SEQ ID NO: 99
Function: Fixation of CO₂
Code: BMS1032
Gene: ppc
Sequence: CAG GAG GTC ATG CTC GGC TAT TCC GAC TCG TGC AAG GAC GGC GGC ATC CTC GCG TCG AAC TGG AAC CTC TAT CAG GCG CAG CTT TCG ATC ATC GCA CTG A
SEQ ID NO: 100
Function: Fixation of CO₂
Code: BMS9
Gene: prk
Sequence: GGG CGC TGT CGA TGT AAC CAA TTA CGT CGA TCT GCT GGT GGG TGT GGT GCC GGT GGT CAA CTT GGA GTG GAT TCA GAA GAT TCA CCG TGA CAA TGC CCA G
SEQ ID NO: 101
Function: Fixation of CO₂
Code: BMS1098
Gene: prk
Sequence: GCC GAA GCC GAA GGC AAT TTC TCG TTC AGC CAC TTC GGC CCG CAG GCC AAC CAT TTC GAC AAG CTC GCG GAA CTG TTC AAG ACC TAT GGC GAG ACC GGC G
SEQ ID NO: 102
Function: Energetic metabolism
Code: BMS10009
Gene: aaC
Sequence: GTC GAC AGT CAT ATT TAT GCG GGT TAT CGG GTA CCR CCT TTC TAT GAY TCC ATG ATC GGY AAA ATC ATC GCC TTC GGG GGT GAC CGC GAG GAA GCC AAC A
SEQ ID NO: 103
Function: Energetic metabolism
Code: BMS10000
Gene: aaC
Sequence: GGG GCA TGC GGA TCG TCC ATC AGG AAA GTG AAT TCG AAA ACG CTC TCC ATG CAG CCC AGA CGG AAG CTT TTC AGG CCT TCG GAT CGA AAG AGG TTT ACA T
SEQ ID NO: 104
Function: Energetic metabolism
Code: BMS564
Gene: aaC
Sequence: GGT GGC CGA AAT TTC GGC CAA GGG ATA TAA CCC GCA TGT GAA GCT TGA CGA GTA CCG CAC CCG CGA GAC TCC GCG CAT GCC TGA TTT CAG GCC TGT GCT G
SEQ ID NO: 105
Function: Energetic metabolism
Code: BMS10001
Gene: aaC
Sequence: CTG TCG TTC CCG GAA GTC AGG GAG CGA TCT CGT CTG AAG AGG AAG CCA TCA CGC TCT CCC GAG AAA TCG GCT ATC CCC TGA TCA TCA AGG CTT CCG CAG G
SEQ ID NO: 106
Function: Energetic metabolism
Code: BMS10235
Gene: aaC
Sequence: CGA GAC AGT GTT CTC CGA TGT AAG CAG AGA RTT YTA YTT CCT TGA RTT AAA YAA RAG RYT ACA AGT TGA ACA TCC AAT AAC AGA GAC CAT ATT CAG AAT A
SEQ ID NO: 107
Function: Energetic metabolism
Code: BMS748
Gene: aaC
Sequence: GGG AGG TGG AGC AGG AGT GGG TAT TAT AAA GGT TGA TAA TCC TAG TGA ACT AGC TGA GGC TTT TGA AAG AAG CAA AAG ATT AGC GTA CTC TGC CTT TGG C
SEQ ID NO: 108
Function: Energetic metabolism
Code: BMS10331
Gene: aaC
Sequence: CGT CGG GYT TCA CGT TCA TCG GCC CGC GCC CCG ACA ACA TCC GCC TGA TGG GCG ACA AAG TCG CGG CCA AGC AGG CCA TGA TCG ACG CCA AGG TGC CCT G
SEQ ID NO: 109
Function: Energetic metabolism
Code: BMS10034
Gene: accA
Sequence: TAT TAT CGG CGG TCT GGC GCG CTT CAA TGG ACA GCC GAT CGT CTG GAT GGG ACA TCA GAA GGG CCG YGA CAC YAA GGA AAA RAT YCA GCG YAA YTT TGG G
SEQ ID NO: 110
Function: Energetic metabolism
Code: BMS271
Gene: accA
Sequence: GGG CCC AGG AAA GGC CGA CGG CTC TCG ACT ATA TCC AGC GGC TCT GCA CCC AGT TCA TCG AAA TTC ACG GTG ACC GTT CCT TCA GGG ATG ACC CTT CCA T
SEQ ID NO: 111
Function: Energetic metabolism
Code: BMS10087
Gene: accA
Sequence: GGC GAC CGC GCG TTC GCG GAC GAC CAG TCG ATC GTC GGC GGC CTT GCT CGC TTC AAC GGC CAT GCG TGC ATG GTG ATC GGC CAT CAG AAG GGC CGC GAT A
SEQ ID NO: 112
Function: Energetic metabolism
Code: BMS897
Gene: accA
Sequence: CGA TCC TGA CCT TCA TCG ACA CCC CGG GCG CCT ATC CCG GGA TCG GTG CAG AGG AGC GCG GCC AGT CGG AAG CGA TCG CCC GCA ACC TCT ATG TCA TGG C
SEQ ID NO: 113
Function: Energetic metabolism
Code: BMS99
Gene: accB
Sequence: CCA GGG CTA TAT GAT CAA ATC CCC CAT GGT CGG GAC TTT TTA TCG CGC ATC CTC TCC GGA ATC TCC ACC CTT CGT GGA AGA AGG CAG CAT GGT GAA GGC C
SEQ ID NO: 114
Function: Energetic metabolism
Code: BMS204
Gene: accB
Sequence: ATG GAT ATC CAA TTC ATT CGT CGC CTT GCC GAA TTA CTT GAA AAA AGC GCT ATT GAC GAA ATT GAA GTT GTG GAA GGT GAA AGT AAG GTG CGT ATT ACC C
SEQ ID NO: 115
Function: Energetic metabolism
Code: BMS342
Gene: accB
Sequence: GAA TCC GGC ATC TCC GAA CTG GAA GTC ACC GAA GGC GAA GGT AAG GTG CGC ATC GTC AAG AAC GCG CCG CCG GTC TAT GTG CAG CCG TCG GCC GGC TTC G
SEQ ID NO: 116
Function: Energetic metabolism
Code: BMS458
Gene: accB
Sequence: CAT CTC CGA ACT GGA AGT CAC CGA AGG CGA AGG TAA GGT GCG CAT CGT CAA GAA CGC GCC GCC GGT CTA TGT GCA GCC GTC GGC CGG CTT CGC GCC GCA G
SEQ ID NO: 117
Function: Energetic metabolism
Code: BMS986
Gene: accB
Sequence: ATG GAT CTC AGA AAA CTG AAA AAG CTC ATT GAT CTG GTC GAG GCC TCC GGC ATC GCC GAA CTC GAA ATC ACC GAA GGC GAG GAA AAG GTC CGC ATC GCC A
SEQ ID NO: 118
Function: Energetic metabolism
Code: BMS10039
Gene: accD
Sequence: CTG GAT TTT TTC CTG GAT GCA GAA CCG CGC CTG GAA ATT GCC AAG GAA TAT GTC CCC GTT GAT CCC CTG CGY TTC AAR GAT CAG AAG CGC TAY AAG GAG C
SEQ ID NO: 119
Function: Energetic metabolism
Code: BMS217
Gene: accD
Sequence: GGG TTC CTG AGG GAT TGT GGA TCA AGT GTT CCC TCT GTC GAC AAA TTG TCT ACC GGA AAG AAG TCG AAA AAG CAG GAA AAG TCT GTC CGA AAT GCA ATT A
SEQ ID NO: 120
Function: Energetic metabolism
Code: BMS10215
Gene: accD
Sequence: CCC GAG GGC CTG TGG GTC AAG TGC CCG TCG TGC GAG GCC GTG CTG TAC CGC AAC GAC GTC GAT GCG AAT CAG CAC GTG TGC CCG AAG TGC GAT CAC CAC A
SEQ ID NO: 121
Function: Energetic metabolism
Code: BMS694
Gene: accD
Sequence: GGG TCT CGA AGA TGC CGT CAA GGT CGG AGA GTG TCG GAT CGA GGG AAT CCC GAC CGT TCT TGG TCT CTT CTC CTT TCA TTT CAT GGG AGG ATC TAT GGG G
SEQ ID NO: 122
Function: Energetic metabolism
Code: BMS928
Gene: accD
Sequence: CCC AGT TGT CGC GCA ACC GGC TGC CCT TCG TTT CGG TGC TGA CCG ACC CGA CGA TGG GTG GCG TTT CCG CCA GCT TCG CGA TGC TCG GCG ACC TGA TCG T
SEQ ID NO: 123
Function: Energetic metabolism
Code: BMS101
Gene: gltA
Sequence: GGA ACC GAA CTT TGC GCC AGG TCT GGA GGG TGT GGC TGC AAC CCA GTC CAG CAT TTC CAA CAT CGA TGG CGC TGC CGG CCT GCT GAG TTA CCG TGG TTT T
SEQ ID NO: 124
Function: Energetic metabolism
Code: BMS10147
Gene: gltA
Sequence: ATG ACC CCG TCT GAT GTT AAA GCC ACG CTA TCG TTC AGC GAT AAT TCG CCG AGC GTC GAA CTG CCG ATC TAC AAG GGT ACG ATG GGC CCC GAC GTG ATC G
SEQ ID NO: 125
Function: Energetic metabolism
Code: BMS10229
Gene: gltA
Sequence: CTC TTT GAA ATT GCA GTR AAA CTY GAG GAA CTT GGT GTA AAR GAR TTC GGT GGC AAA GGG AAT ATA TCC AAT ACA GAT TAT TAT TCC GGC ATA GTT TTC A
SEQ ID NO: 126
Function: Energetic metabolism
Code: BMS723
Gene: gltA
Sequence: TAA GAA GTA TCT TGA CAT TGC TGA AAG ACT TGA AAA GTT AGG AGT AGA AAC ATT CTC AGG TAA AGG AGT CTA TCC AAA TAC AGA CTT CTA CTC AGG GAT T
SEQ ID NO: 127
Function: Energetic metabolism
Code: BMS755
Gene: gltA
Sequence: TAT AGA AGA AAG GAG GGT AAT AAG CCA AGA ATT CCA GAG CCC TCA GAT AGT TTT GCA AAA AGC TTT CTT TTA GCG AGT TTC GCT AGG GAA CCT ACT ACA G
SEQ ID NO: 128
Function: Energetic metabolism
Code: BMS804
Gene: gltA
Sequence: ACG CAT CAA CCT TTG CCT CAT TAG TTG TAG CCT CTA CTT TTT CAG ATT TAT ATT CTT CTA TAG TAG CTG GGA TAT CAG CAT TGA AAG GGC CTT TGC ACG G
SEQ ID NO: 129
Function: Energetic metabolism
Code: BMS10243
Gene: gltA
Sequence: CAT CGG ARA YAA AGT TCA AGT GGA AYA AGG ATA CGG ACA GRG AYG TTG CAG CAG AAA TGA TCG GCA GGA TGT CAG CGA TAA CCG TGA ACG TGT ACA GGC A
SEQ ID NO: 130
Function: Energetic metabolism
Code: BMS1014
Gene: gltA
Sequence: ACG AAG GCC TGC TGT ACT ACC GGG GCT ACC CGA TCG ACC AAC TCG CCT ACC AGT CGG ACT TCA TGG AAG TCA GCT ACC TGC TGC TGC ACG GCG AAC TGC C
SEQ ID NO: 131
Function: Energetic metabolism
Code: BMS50
Gene: idh
Sequence: GTG ACC ATG GCG CAT GTT CAG ATT CCG GCT CAG GGG AAG CCC ATC ACC CAG GTG GAC GGG GTG CTG CGG GTG CCC GAT CAA CCG ATT ATT CCC TTC ATC G
SEQ ID NO: 132
Function: Energetic metabolism
Code: BMS275
Gene: idh
Sequence: GGG TCA GGA TAT CTG GAA CGC CTC CGT GCG CGT GTT CGA TGC CGC TGT CGA AAA AGC TTA CAA GGG CAA GAA AAA AGT GGT CTG GTT CGA AGT GTA TGC C
SEQ ID NO: 133
Function: Energetic metabolism
Code: BMS542
Gene: idh
Sequence: CCC GTG GGC AAG GGG TTC CGC AGT CTC AAC GTG ACC ATG CGC CAG ACT CTC GAC CTG TAT GCC TGC ATC CGG CCC ATA CGG TAT TTC GAA GGC ATC GAA T
SEQ ID NO: 134
Function: Energetic metabolism
Code: BMS646
Gene: idh
Sequence: AGA GTG GAA AGA AAT TCT TGT AGG GGA CAA AGC ACT GAA AGA GAA AAA TGA TCG TTT CCC GGA AGA ATC ACA GGA GGC AAT AAA ACA ATA CCG TGT ACT G
SEQ ID NO: 135
Function: Energetic metabolism
Code: BMS669
Gene: idh
Sequence: GGG AGT GAT GAT GCT CCG CTG GAT CGG TGA AAA TGC CGC CGC GGA CAA GAT CGA GAA GGG CAT GAA CAA GGT TCT GGC CGA GGC CAA GAC CCT GAC CTA C
SEQ ID NO: 136
Function: Energetic metabolism
Code: BMS742
Gene: idh
Sequence: ACA AAA GAA AGT CAC CCA GGA TAT AGC GAG ATA CCT AGG TAT CAC ACC TCT AGG AAC AAA GGA ATA TAC TGA CAC ACT TGT GCA AAT AAT GGA CTC CTT C
SEQ ID NO: 137
Function: Energetic metabolism
Code: BMS778
Gene: idh
Sequence: GTG GGT AGA AGT TCT AGC AGG AGA TAA GGC AGA AAA GCT AAC CGG AAA TAG ATT TCC TAA AGA GTC AGA GGA ATT GAT AGA GAA GTA TAG AGT CTT ATT A
SEQ ID NO: 138
Function: Energetic metabolism
Code: BMS823
Gene: idh
Sequence: GGC ATA TAT TCA AGT GAA GGA GGA TGG AAC CCT TCA GGT TCC AGA TAG AGT AAC TAT AGG ATA TAT AGA GGG CGA TGG AAT AGG ACC TGA TAT ATC AAA G
SEQ ID NO: 139
Function: Energetic metabolism
Code: BMS858
Gene: idh
Sequence: GGG GAT GGT ATA GGC CCC GAT ATC ACG AAG ACC ACG ATA AAT GTA ATA AAT GCT GCC CTT GAG GTC GCC TAT GGT GGA AAG AGA TCA ATA GAA TGG CAC A
SEQ ID NO: 140
Function: Energetic metabolism
Code: BMS916
Gene: idh
Sequence: CTG GAT GGA GGT CTT TGC CGG CGA GAA AGC CGT CAA GGT CTA CGG CGG CGA CCA GTG GTT GCC CGA AGA AAC CGT TTC CGC CGT CAG GGA CTA CGT GAT T
SEQ ID NO: 141
Function: Energetic metabolism
Code: BMS57
Gene: pdhA
Sequence: CAA CGG TCG AGC GTA TGC GCC AAG AGG TTC AGG AAG AAG TGG CAG ATG CGG TCA CTT TTG CCG AAC AGA GCG CCG CAC CGG ATA TGG AGG ATG CCT GGA A
SEQ ID NO: 142
Function: Energetic metabolism
Code: BMS10222
Gene: pdhA
Sequence: GTC CGG GAT GCA AGA CCG ATT TTT CTT ACC TGC GCC TGT CGC CAG CCG GCA AGG TGC GCA AAC CCC CCA TCG ACG TCG CCC CCG CCG ACA CGA GCG ACT T
SEQ ID NO: 143
Function: Energetic metabolism
Code: BMS758
Gene: pdhA
Sequence: GGG ATT TGT GCA TCT CTA TGT AGG TGA AGA GGC TGT AGC TGT AGG GGT TAT GAG TAC GCT AAG AGA TGA TGA CTA TAT AAC TAG TAC GCA TAG AGG ACA T
SEQ ID NO: 144
Function: Energetic metabolism
Code: BMS819
Gene: pdhA
Sequence: CAG ATC TGG AAA TCC GAT TCT GGT GGA GGC GAG AAG CTA CAG GAT GGG GCC GCA TTC AAC ATC GGA CGA TCC CAG CAA GTA CCG CAA GAA CGA GGT GCA G
SEQ ID NO: 145
Function: Energetic metabolism
Code: BMS854
Gene: pdhA
Sequence: GTG TTT ACG TTG ATG GAA ACG ACT TCA TAA AAA CTT ACA ATG CCG TAA AGG AGG CTG TTG AAT ACG CAA GAT CAG GAA ATC CGA TAC TTG TCG AGG CCA G
SEQ ID NO: 146
Function: Energetic metabolism
Code: BMS115
Gene: pdhB
Sequence: GTG GTT CCC GCT ACT CCG CAG GAT GCC TAC TGG CAG TTG CGC CAA GCC ATC CGC AGC GAT GAT CCC GTG ATA GTT CTC GAG CAT GAG TTG CTC TAT TTC G
SEQ ID NO: 147
Function: Energetic metabolism
Code: BMS138
Gene: pdhB
Sequence: CGG CGA TGC TGG GTC TGC GCC CGG TGG TCG AAA TCA TGA CCA TCA ATT TTG CTT TGT TTG CCA TTG ACG CCA TCA TCA ACA TGG CCG CCA AGA TCC CGT T
SEQ ID NO: 148
Function: Energetic metabolism
Code: BMS225
Gene: pdhB
Sequence: AGA TGC AAA GGG ACT GAT GCT TTC CTC CAT CGA GGA CAA CAA TCC TGT CCT GAT CTT TGA ACA CCG CTG GCT CAT GAA AAG GAA AGG GGC CGT TCC GGA A
SEQ ID NO: 149
Function: Energetic metabolism
Code: BMS10180
Gene: pdhB
Sequence: GCG GCT CTA CAA CGG CCC GTT CGA CGG CCA CCA CGA ACG GCC GGT GAC GCC GTG GAG CAA GCA TCC GGC GAG CCT CGT GCC CGA CGG CTA CTA CAC GGT G
SEQ ID NO: 150
Function: Energetic metabolism
Code: BMS637
Gene: pdhB
Sequence: TAT TTT TGA TAA AAA GGT TAA TTT CGC CTG TGC CGG AAT TGT AGG TTC ATC TTT TCC CTC TGC ACT TGG AGC AGC CCT GGC ATC GAA ACT CGA TGG TAA A
SEQ ID NO: 151
Function: Energetic metabolism
Code: BMS653
Gene: pdhB
Sequence: ATG ACT CAG ATG ACA ATG GTA AAA GCA CTG AAC AGC GGT TTA AAT AAT GCC ATG GAG AGA GAT GAT TCC ATC ATA CTT CTG GGA GAA GAT GTT GGT ACC G
SEQ ID NO: 152
Function: Energetic metabolism
Code: BMS704
Gene: pdhB
Sequence: ACC ACG GAG GGT CTT CTG GCA ACG TAC GGA GAT TGG CGG GTC CGG GAC ACG CCC ATC TCC GAG AAC AGC TTT ACC GGC CTC GGC GTG GGA GCG GCC ATG G
SEQ ID NO: 153
Function: Energetic metabolism
Code: BMS750
Gene: pdhB
Sequence: ATA CAC CCA TTA CTG AAC AAA CAT TTA TGG GCA TTA GCG TTG GCG CTG CTT CCT CTG GCT TAC ACC CAG TTG TTT CAT TAA TGT TCG TAG ACT TTC TAG G
SEQ ID NO: 154
Function: Energetic metabolism
Code: BMS814
Gene: pdhB
Sequence: ATG AAC ATG GTT CAG GCA CTG AAC AGT GCA ATG GAT CTC AAG ATG TCC GAG GAT GAC AGC GTA ATA ATA CTC GGA GAG GAC GTA GGC AGG GAT GGA GGT G
SEQ ID NO: 155
Function: Energetic metabolism
Code: BMS865
Gene: pdhB
Sequence: CAT TTA CAC CGC AAT GGA TCA AAT AAT AAA TCA GAT GGC AAA GAT AAG GTA CAG ATC TGG TGG AGA TTA CAC AGT GCC GCT TGT CCT TAG GAC TCC TGT T
SEQ ID NO: 156
Function: Energetic metabolism
Code: BMS1041
Gene: pdhB
Sequence: CTT CGG CTA TAA CAT TTC TGT GCA CTG GTG CCT GAA AGC CGC CGA AAT CCT CGA CAA GCA GTA CGG CAT CTC GGC CGA GGT CGT CGA CCT TTA TTC GCT C
SEQ ID NO: 157
Function: Chemiotaxis and mobility
Code: BMS149
Gene: flhA
Sequence: GAT AAA AGC GTG GCT TTG GGG TAT ACC GTA GTG GAC CCG GCC ACC GTC ATT GCC ACC CAG TTG CAC CAG ATT CTC CAA AGC CAT GGT GCG GAC TTA CTG G
SEQ ID NO: 158
Function: Chemiotaxis and mobility
Code: BMS264
Gene: flhA
Sequence: GGG AAA AAC AGG AGT TTG CCG ATC ATC AGT TTT GAC CGG AGA TGG GAA GAT GTG TTT CTC AAG CAG GCC GCA CAG ACA CCG CAG GGA TCG CCC CTC GCC C
SEQ ID NO: 159
Function: Chemiotaxis and mobility
Code: BMS10328
Gene: flhA
Sequence: GGG ATG GCT CAG CAC GAC ATG AGC TTC GCC GCG GCG GGC AAG AAC TAC ACG CTG CTC ACG ATC GGC GAC GGC CTC GTC GCG CAG ATC CCG TCG CTC GTG A
SEQ ID NO: 160
Function: Chemiotaxis and mobility
Code: BMS612
Gene: flhA
Sequence: TGT GAC TTC CAT GTT CAT GCT TTC CCC GCT TGA ATT TTC CAT TTT TCC TTC GCT TCT GCT GGT TAC CAC GCT GCT GCG TCT TGC TCT TAA CGT GGC AAG T
SEQ ID NO: 161
Function: Chemiotaxis and mobility
Code: BMS144
Gene: flhB
Sequence: GGG AAA TGG CGC GCC GGC GGA TGA TGG CAG CCA TTC CTA AAG CCG ATG TCA TTG TGA CCA ACC CAA CCC ACT ATG CCG TTG CCC TGC GTT ATA AAG AAG G
SEQ ID NO: 162
Function: Chemiotaxis and mobility
Code: BMS265
Gene: flhB
Sequence: AGC CGC GAG GTC GGT TCG CTG TTT TTT CTG ATC TCG GCC CTT CTC CTG TTC GGC GCG ACC GGA CAC GGC ATC ATG GTC AGT CTT GAA GAC CAG ATG GCC A
SEQ ID NO: 163
Function: Chemiotaxis and mobility
Code: BMS10113
Gene: flhB
Sequence: GCA CCA TGC TGC ACA CCG CAT TCT CGT TCG ACC GCG CAG CCG CGT TCG ATA CGA ACC GGA TGC TGT CGC ACG CGG GCA CGC TGA GCC TCG AAG GGC TCT A
SEQ ID NO: 164
Function: Chemiotaxis and mobility
Code: BMS631
Gene: flhB
Sequence: CAA GCT GGC GCT CAT GCT GCT GCC CAT CAT GCT GTT TCT CGC GCT TGT GGC GTA TGT GGT CAT CCG TCT GCA GGT GGG CAA ACT GTG GGC GCC CAA AGT T
SEQ ID NO: 165
Function: Chemiotaxis and mobility
Code: BMS983
Gene: flhB
Sequence: CTG GCC AAG GTC GGT CTG CTC GGC GGC GTT GCC GCA TGG CTC GTC TGG TCG AAC GTC GAG GCG ATC GTG AGC CTG AGC CTC GAA GCA CCC ACG GCC GCG A
SEQ ID NO: 166
Function: Chemiotaxis and mobility
Code: BMS202
Gene: fliF
Sequence: ATG CCA AAC GTA TTT ACA CGT TCA GGA GAC CTG CGC AAG AAA GCT ATG CAA TGG TGG TCT GAT AAA AGT CAG CAA CAC AAG TTA TTT ATT CTC ATA GGC G
SEQ ID NO: 167
Function: Chemiotaxis and mobility
Code: BMS238
Gene: fliF
Sequence: GGG AAA GTG GCT TCT CCG CCG CTG GGG CCG AAC AAT ACC TCG GAA ACG CGC TAC TCC AAG AAA AAA CAG ATC GCC CAT TAT CAT GTC AGC CGG ACG GAC C
SEQ ID NO: 168
Function: Chemiotaxis and mobility
Code: BMS10124
Gene: fliF
Sequence: AAC AGC GCG TTC TCG GCC GCG GCC GAT CCG TTC GCG AAC CTG CCG TGG TGG CGC CAG CCG GAC ATG ATC GAA CTC GGC AAG GAC ATC GCG AAA TGG CTG G
SEQ ID NO: 169
Function: Chemiotaxis and mobility
Code: BMS582
Gene: fliF
Sequence: GGG CAA GAT TCT GTA CTA CCC CAA AGA CGA ACA GTC GCT TGA CGG CAT GAC CTC CAC CCA GCT GGA ACA CAA GCT GAC CAT GCA GCA GAA TTT CGA ACG C
SEQ ID NO: 170
Function: Chemiotaxis and mobility
Code: BMS687
Gene: fliF
Sequence: CCC CTT GGG CCC CAA GGA TGG AAC GCA GAC GAA ATA TTC GAA GAA GAA GGA TAT CGA ACA CTA CGA CGT GAG CCA TAC CAT GAG CCA CAT CGT TCT CCC C
SEQ ID NO: 171
Function: Chemiotaxis and mobility
Code: BMS933
Gene: fliF
Sequence: TCA CCG TCG TCG ACC AGA ACG GCA ATC TGC TGT CCG ACG CCG GCA AGG CCC CGC GCG TCA ACG GCA TGG ATC CGA GCC AGA TCA AAT ACG TGC AAG AGC T
SEQ ID NO: 172
Function: Chemiotaxis and mobility
Code: BMS179
Gene: fliG
Sequence: ATG GCT GAT CTG ACA GGC CGT ACA GGC ACG TTG AAT AGT GGG TTG GAT TAT GCC GCA GTC TTT CTC CTG GGC GTC GGC CCA GAT GAT GCT TCT TTG ATT A
SEQ ID NO: 173
Function: Chemiotaxis and mobility
Code: BMS288
Gene: fliG
Sequence: GGG AAG GAG CAG GCG GAC AAG ATT CTC GAG ATG ATG AAC GAT CAG GAA GGG AGC AGT CTC CAG TCG CTG AAG TGG ATG GAT CCC AAA TCC ATC GCC AAC C
SEQ ID NO: 174
Function: Chemiotaxis and mobility
Code: BMS10110
Gene: fliG
Sequence: CTG AAG GCT TGA ACA AGA GCG CGC TCC TGC TGA TGT CGA TCG GCG AGG AAG AGG CCG CGC AGG TAT TCA AAT TCC TCG CGC CGC GCG AAG TGC AGA AGA T
SEQ ID NO: 175
Function: Chemiotaxis and mobility
Code: BMS528
Gene: fliG
Sequence: CTT ATC AGC AAC CTG CCG GCA GGT GTG CGC CCC GAA GTG CTG ATG CGC CTT GCC AAA CTG GAA GCA GTG CCC GAA GAC ATG CTG ATG GAA GTG GAC AAG G
SEQ ID NO: 176
Function: Chemiotaxis and mobility
Code: BMS675
Gene: fliG
Sequence: TCA CGA GTC TCG GCG TCA GCC GGA TCG AGA AGA CGG CGA ACC TTT TGA ACG AGA TGG ACC GCT CCA CCT CGG AGG GGA TCC TCG ACT TCA TCA GCC AGA A
SEQ ID NO: 177
Function: Chemiotaxis and mobility
Code: BMS961
Gene: fliG
Sequence: CAA CTT CAT GAG CGG CGA AAA CGA GGC AAT CGC GAT GGA GTA CCT GAA AAG CTA CGA CCC CGA CAT GGC GCA GAA GAT CAT GGA CGA GAT GTT CGT GTT C
SEQ ID NO: 178
Function: Chemiotaxis and mobility
Code: BMS150
Gene: fliM
Sequence: ATG GTA CAG GAT ATT CTT TCT CAG GAT GAA GTG GAT GCC CTA TTT AAG GGC ATG TCA GAT GGT GAT GTC GAA ATG CAC GCT GAA CCT GAG CCT GAT CCG G
SEQ ID NO: 179
Function: Chemiotaxis and mobility
Code: BMS316
Gene: fliM
Sequence: TGG AAA ACC GGA GAT GTC TTT TCC CTG GAC CGG TAT GTC AGC GAG CCT GTG GAG GTT CGC GTG GAG GGC ATC CTC CGC TTT CTG GGG AGG CCC GGA ATT T
SEQ ID NO: 180
Function: Chemiotaxis and mobility
Code: BMS10091
Gene: fliM
Sequence: GCC TCG AAA TCA TCA ACG ATC GCT TCG CGC GCC TCT TGC GGA TCG GCA TCT TCA ACT TCA TGC GGC GCA CGG CGG AAA TCT CCG TGA GCC AGG TGA AGG T
SEQ ID NO: 181
Function: Chemiotaxis and mobility
Code: BMS628
Gene: fliM
Sequence: CTG CCA TTG TGC CGC CCA GTG ACG TTG TTG TGG TCA TCA CCT TTG AGG TGG AAC TGG AAT CGG CCA TCG GTT CGC TGG TTA TGT GTC TGC CGT ATG CCA C
SEQ ID NO: 182
Function: Chemiotaxis and mobility
Code: BMS665
Gene: fliM
Sequence: CCC CGT CTC CAC GGA GAT GAT CAA GTT TGG GGA GTT CAT CAA GAA AAT CCC TCT CCC GTC GAA CAT CAA CAT TCT TCG CCT CGA ACC GAT GAA ACG CAA C
SEQ ID NO: 183
Function: Chemiotaxis and mobility
Code: BMS1011
Gene: fliM
Sequence: ATG CGC ATA CGG AAA ATT CAA CGG CCA GTA CAC CCT GCG CGT CGA AAA GCT GTT GTC GAC CAG CGC GGT CGA ACC GAA AAC AGG AGA GCA GCA TGT CTG A
SEQ ID NO: 184
Function: Chemiotaxis and mobility
Code: BMS332
Gene: fliN
Sequence: GAT CGC GCA GGG CGA AGT GGT GGT CGT CAA CGA CAA GTT CGG CAT CCG GCT CAC CGA CAT CAT CAC GCC GTC CGA ACG CAT CCG GAA GCT GAA CCG ATG A
SEQ ID NO: 185
Function: Chemiotaxis and mobility
Code: BMS472
Gene: fliN
Sequence: GAT CGC GCA GGG CGA AGT GGT GGT CGT CAA CGA CAA GTT CGG CAT CCG GCT CAC CGA CAT CAT CAC GCC GTC CGA ACG CAT CCG GAA GCT GAA CCG ATG A
SEQ ID NO: 186
Function: Chemiotaxis and mobility
Code: BMS1079
Gene: fliN
Sequence: GAA GAC ACC ACG CAA ACG ATC GCC GAG GAC GAT TGG GCC GCG GCC ATG GCC GAG CAG GCC GCG CCG GCC GCC GCC CCC GCC CCC GAG ATC TTC AAG GAA T
SEQ ID NO: 187
Function: Chemiotaxis and mobility
Code: BMS216
Gene: motA
Sequence: GGG AGA TGG AAA TTC CCA TTC ATG CCC TGG TTC GTA CCG CCG ACG GAA TGC CTG CCT TCG GTA TTG TTG CTG CGG TAA TGG GCG TGG TAA ATA CCA TGG G
SEQ ID NO: 188
Function: Chemiotaxis and mobility
Code: BMS10097
Gene: motA
Sequence: CAT CAT CGG CAA CGG CAT CAA GAC GAT CAA GGC GAC GCT GCG CGT GCT GCC CAC GCT GTT CAA GGG CTC GAA GTA CAA CAA GGA CAT CTA CAT GGA GCT G
SEQ ID NO: 189
Function: Chemiotaxis and mobility
Code: BMS1082
Gene: motA
Sequence: CCT TTC TCG TCG GCA ACA ACA ACA AGG TGA TCA AGG CGA CGC TGA AGG CCT TGC CGA CCG TGC TCA AAG GCT CGA AGT ACA CGC GCG CGC TTT ACA TGG A
SEQ ID NO: 190
Function: Chemiotaxis and mobility
Code: BMS195
Gene: motB
Sequence: GGG ACG TGC CAA CGC TGT TCG CCA GGT GCT GGT TCA GAA TGG TCT GGA TGA AAG TAA AAT ACT GAG AGT AGT CGG CAT GGG ATC GGC GGT GTT GTA CGT G
SEQ ID NO: 191
Function: Chemiotaxis and mobility
Code: BMS10108
Gene: motB
Sequence: AGG TGA AGC TGA TGG CRG CCA TCG AGG CGA ATC CGA CGC TGC GCC AGT TCA AGC AGC AGA TCC GCA TCG ATT CGA CGC TGA TGG GGC TGC GCA TCG AAA T
SEQ ID NO: 192
Function: Chemiotaxis and mobility
Code: BMS912
Gene: motB
Sequence: GGC CAA TGT TCA ACC TCG CGA GCG CCG AGT TGC AGC CCT ATA CCA AGG TCA TCC TGC ACG AGA TCG CAC AGG TCC TCA ACG ACG TCG AAA ACC GCA TCA G
SEQ ID NO: 193
Function: Iron oxidization
Code: BMS10031
Gene: cyc1
Sequence: ATG ATG GAA ACC GGG TGA ATC CCA TCA TGC AGC CCG TGG CGG AAA AGC TGA CCA ACC GGC AAA TGC GGG AGG TGG CGG CTT ATC TGT CCG GTC TGC ATT G
SEQ ID NO: 194
Function: Iron oxidization
Code: BMS10057
Gene: cyc2
Sequence: GGG TAT CGG CAC TTA CGA AGC CTA TAT TTT TGG GCA GGA TAA GGC CAA CTG GCT CTA CTT CGA GGC GGA CGG ATA CAC CAA CGC GGA CGG TAT TGG TGT A
SEQ ID NO: 195
Function: Iron oxidization
Code: BMS124
Gene: cycA
Sequence: AAG ATG GTG GCC ATG GTA TCT GTC GTG TTT ATT GGT ATG GGT GCG ATG CCT TTT GAC ACA ATT GCT TAT GGA GCG GCA AGC GCA CCG AAC AGC AAG ATT G
SEQ ID NO: 196
Function: Iron oxidization
Code: BMS10013
Gene: cycA2
Sequence: GGG ACA CGT TTA CGA TCC CAA ACT CGT GGC CGA AGG GAA AAA GCT GTA CTT CGG TGG TCT GCC CGA CAA ACA CAT GCC GGC CTG CAT GGC CTG CCA TGG C
SEQ ID NO: 197
Function: Iron oxidization
Code: BMS10042
Gene: rus
Sequence: GCC CAA CAT CAA ACC CAT CGT CGC GGG TAC CGG ATT CAG TCC TGT TCC CAA GGG TGG AAA GTT CGG RTA YAC GGA YTT YAC CTG GCA TCC GAC RGC CGG C
SEQ ID NO: 198
Function: Nitrogen metabolism
Code: BMS10066
Gene: narH
Sequence: ATG AAA GTA CGT GCA CAA TTT GCT CTC GTT TTT AAT CTA GAT AAG TGC ATC GGT TGT CAT ACA TGC TCG GTG ACC TGC AAA AAT ACC TGG ACC AAC CGT C
SEQ ID NO: 199
Function: Nitrogen metabolism
Code: BMS10100
Gene: narH
Sequence: AAC TGG GAA GAC GAC CTC GGC GGC GAG TTC AAG TCG CGC AGC CGC GAC AAG CTG TTC GAG GCC GTG CAA AAG GAG ATG TAC TCG ACG TTC GAG AAC ACC T
SEQ ID NO: 200
Function: Nitrogen metabolism
Code: BMS999
Gene: narH
Sequence: ATG AAA GTC AGA GCG CAA TTC GCC TTC GTC TTC AAC CTG GAC AAG TGC ATC GGA TGT CAC ACC TGC TCG GTG ACC TGC AAG AAC GTC TGG ACC AAC CGC A
SEQ ID NO: 201
Function: Nitrogen metabolism
Code: BMS10064
Gene: nifK
Sequence: GCG GAA GTG ATC GGT GAC GAC CTG AAC GCC TTC ATC AAA ACC TCC AAG GAA AAG GGC AGC GTA CCA GAA GAA TAT GAT GTG CCT TTC GCC CAT ACT CCG G
SEQ ID NO: 202
Function: Nitrogen metabolism
Code: BMS698
Gene: nifK
Sequence: AAA AAG GAT CGG TTC CCC AGG AAT ACG ATG TTC CCT TTG CCC ATA CCC CGG CCT TCG TGG GGT CGC ACA TCA CCG GCT ACG ACA ATG CGC TGA AGG GAA T
SEQ ID NO: 203
Function: Nitrogen metabolism
Code: BMS284
Gene: nirA
Sequence: GGG GCC TGC GGA GAC GAT ACG AGA AAC ATC ACC ACC TGC CCG ATG AGC GGT CTG GAT CCC CAT CCC GAA AAC AAT TTT TCG GAT CTG GTC GAA GAA GCG A
SEQ ID NO: 204
Function: Sulfur assimilation
Code: BMS76
Gene: cysI
Sequence: CAG GTG TTG GCC ATA GCC GAA GCC GTG CTC ATT ACC CAG CGT GAC CAC GGA GAC CGA AGC AAT CGA AGC CAC GCC CGC CTC AAA TAC ACG GTA GAC CGT A
SEQ ID NO: 205
Function: Sulfur assimilation
Code: BMS737
Gene: cysI
Sequence: TAG AGA AAT ATC AGT TGT ATG TTG GTG GAG GAA TGG GCG AGA ATA ATG GTT ATC CGA CAT TTT CTG CTT TAG CAC TTC CTT TGG GTA CAG TAA GTG AGG A
SEQ ID NO: 206
Function: Sulfur assimilation
Code: BMS772
Gene: cysI
Sequence: CTG GAT ATC CCT CAC CAT CTC TAA AAC TGA CTA CGA GAC AAG CTA TTC AAT TTC ACC ACG TAA AGA AAA GGG ATT TAC CTA ACC TTA TAA GAG AGG TCG C
SEQ ID NO: 207
Function: Sulfur assimilation
Code: BMS800
Gene: cysI
Sequence: TAG ATG CTA TAG TTA GGA TAC AAC AAG AAT GGG GTG ATA GAA AGA ATC GTC ATT GGG CTA GGT TGA AAT ATT TAG TAT ATA AAA TGG GAG TAG AAT GGA T
SEQ ID NO: 208
Function: Sulfur assimilation
Code: BMS46
Gene: cysJ
Sequence: GGG GTT GCC TCG ACA TTT CTG GCG GAT CGT GTC GCC ATC GGC CGG CCT GTA CCC ATA TTT ATC GAA CCC AAT GCC GAA TTC CGG CTA CCC GAA GAT TCC G
SEQ ID NO: 209
Function: Sulfur assimilation
Code: BMS300
Gene: cysJ
Sequence: AAC GGC GTG CAC GGG GTG ACA AGG GCC GCA ACT GGT TGT TCT TTG GCG AGC ACA CGC AGC CAC CGA TTT CTA CTA TCG CGA CGA ACT TGC TAG CTA GCT A
SEQ ID NO: 210
Function: Sulfur assimilation
Code: BMS10168
Gene: cysJ
Sequence: TCG CGG CTC GTC GCG AAC CTG CGG CTC AAC GAG CCG GGT GCG GCG AAG GAC ACG CGC TGC GTG TCG CTG TCG RCG GAC GGC GCG GCG ATC GAA TAC GAA A
SEQ ID NO: 211
Function: Oxide reduction of sulfide compounds
Code: BMS789
Gene: doxA
Sequence: AGG TAC TGG GCA GTG GGC ATA TGG AAA CGT TGT AGG ACC TCT ATT TAA TAA CTC TAA GCT TCC AAA ACT TGA AAT AAC ATA CAT AAG TGC ATC CCC AGA G
SEQ ID NO: 212
Function: Oxide reduction of sulfide compounds
Code: BMS809
Gene: doxA
Sequence: CTC CGC AAG GCT TAT GCT TGA TCA TGA ATA TCA CTG ATA TTG ATG GTC CAG ATG CAT ATC CAG CAT CTG CTC CCT TAA TGG AAA TAT CTA ATG GAA CAT G
SEQ ID NO: 213
Function: Oxide reduction of sulfide compounds
Code: BMS760
Gene: doxD
Sequence: GTG GGA AAG ATG GCA AAT AAA CAA GTC GTA GAT AAC CTA ACT AGA AAG GAA TAC TTG TTC CCT ATA AGG TTC GCA GTA GGT TGG ATG TTC TTC GAT GGA G
SEQ ID NO: 214
Function: Oxide reduction of sulfide compounds
Code: BMS793
Gene: doxD
Sequence: TAT TCT TAG TTA TAG GAC TTT TGA CAA GAT TAG CTG CCT TTG GTG CAG CTG TAA TGG CTA TTG GTA TGG CTC CAG CTT ACT GGT TAG GTT CAA CGT GCG A
SEQ ID NO: 215
Function: Oxide reduction of sulfide compounds
Code: BMS10027
Gene: doxDA1
Sequence: CCC CGG ATC ATC AGG AGA AAG ATC ATG GGT AGT GCA ACG AGC GTA AAC GGC AGT ACA TTT TCA GTG GAC AGA GGA TGG CGC CTG GCG GCG ATA GGG CTG C
SEQ ID NO: 216
Function: Oxide reduction of sulfide compounds
Code: BMS620
Gene: dsrA
Sequence: ATA TCT GGG ACC TGC GCG GTT CCG GCC TGA CCA ACA TGC ACG GCT CCA CCG GTG ACA TCG TTC TGC TGG GTA CCA CCA CTC CCC AGC TGG AAG AGA TTT T
SEQ ID NO: 217
Function: Oxide reduction of sulfide compounds
Code: BMS10336
Gene: dsrA
Sequence: GGC CCT TCG CTG CGG ACC TCG ATG TCC TGC GTC GGC GCC GCA CGC TGC GAG CAT TCC TGT TAT GAC GAG GCC CAT GCG CTG CGC ACT GTC ATC AAC AAC A
SEQ ID NO: 218
Function: Oxide reduction of sulfide compounds
Code: BMS575
Gene: dsrB
Sequence: TGT GCG AAA TCC CGC TGG CCG TTG CTT CCT GCC CCA CCG CCG CTG TTC GTC CCA CCA AGG TTG AAC TGG ACG GCA AAA AGG TGA ACA GCA TCG CCA TCA A
SEQ ID NO: 219
Function: Oxide reduction of sulfide compounds
Code: BMS955
Gene: dsrB
Sequence: TCA AGC TGT CGA CCT CCT GCT GCG AAA TCA ACT GCG GCG GCC AGG CTG ACA TCG CCA TCA TCG TCC AGC ACA CCA AGC CGC CGA AGA TCA ACC ACG ATC T
SEQ ID NO: 220
Function: Oxide reduction of sulfide compounds
Code: BMS978
Gene: dsrC
Sequence: AGG CTA TCT GGT CGA CCT GTC GCA GTG GAA CGA AGA CAT CGC CAA GTA CAT GGC CGT CGA AGA AAA AGT CGA ACT GAC CGA CAG CCA CTG GGA AGT CGT C
SEQ ID NO: 221
Function: Oxide reduction of sulfide compounds
Code: BMS998
Gene: dsrE
Sequence: TCA AGG ACG AAC TCC TTG CCC CCG GAT TCC GGA TCT CCG GAC TGG GTC AGT TGG TCG AGA TGG GCA TTC AGT ACG ATC GTC TCG TGA CGT TCG GTG ATT A
SEQ ID NO: 222
Function: Oxide reduction of sulfide compounds
Code: BMS908
Gene: dsrF
Sequence: TTC ACC GAC GAC GGG ATC TAC CAG TTG ATG AAG GGC ATC GAC ACC AAG GGC ATC GAG GTC AAG GAC TTC TCG AAG ACC TAT CGT GCG CTG GAA GGC TAC G
SEQ ID NO: 223
Function: Oxide reduction of sulfide compounds
Code: BMS579
Gene: dsrK
Sequence: GGG TAT TTA CAC CTT CAT GGG ATA TCT GAT GCT CTT CCA CGA GAT AGG GCT GGA CTA TAC CCT GTC CAC CTA CGC ATC AGA AGG CGG CAA CTT CGG TCT G
SEQ ID NO: 224
Function: Oxide reduction of sulfide compounds
Code: BMS963
Gene: dsrK
Sequence: GTG GCG GCT GCT GAA TTT GAC GTT CCG GAA CTG AAG GAC GAC ATC GAA GTT CCC AAG ATA AGG GAA GGC TCG ATG GCG CAC GCC AAG TCG TTC CTC GCG C
SEQ ID NO: 225
Function: Oxide reduction of sulfide compounds
Code: BMS867
Gene: dsrL
Sequence: ATG GCT GTC ACG ACG AAC AAA GCA GCA AAG ACC GAA GGC ATG ACG TGG CGT CGC TAC AAG GAT GGC GAG TCG GAA GCG AAC ATC CGT TCG GCT CAG GAC C
SEQ ID NO: 226
Function: Oxide reduction of sulfide compounds
Code: BMS547
Gene: dsrM
Sequence: GGG ATT CGT GTA CAG AAT CAT GGA TTG GGC AAA ATC GCC CGT TCC TTT CCG CAT CCC GAC AAC GAG TGG ACA GCA GAA ATC TTT GCC CTG GAT CAA GCC C
SEQ ID NO: 227
Function: Oxide reduction of sulfide compounds
Code: BMS900
Gene: dsrM
Sequence: CCA GAA GGG CGT CAT CTA CCG GAT GGC CAA GGA AGT CGT GCT GTT CGA ATC CCT GTT CAA GTC CAA CAA GTG GAT CTG GGT GTT CGG CTG GCT CTT CCA T
SEQ ID NO: 228
Function: Oxide reduction of sulfide compounds
Code: BMS893
Gene: dsrN
Sequence: TTC TCG CGG GCT ACG TGC ATC GCC GCG GCG CGG GGG CGC GCG GCT GGA TCG TCC CGT TTC TGA ATC AGG TAC GCG CGC ACA AGG CGC GCG TTT CCG CTT A
SEQ ID NO: 229
Function: Oxide reduction of sulfide compounds
Code: BMS952
Gene: dsrO
Sequence: CCC CGA TAG CGA GAT CTC GAA GCG TTT GCG CGA ACT GCC CAC CAA GCA GGT GCG TGC CGA CCT CAA GCT CAA TAC CGG CGT TCG CTA TCA CGG AAT CTA A
SEQ ID NO: 230
Function: Oxide reduction of sulfide compounds
Code: BMS1090
Gene: dsrP
Sequence: AAC TCT ACG GCA GCG CGA TGC TGG CGC CGA TGT TCA TCA TCA TGT CCT TCG CCT ACG GTC TCG CGA TCT ACC TGA TGG TGC TGG TTG CTG CCT ACG CCT G
SEQ ID NO: 231
Function: Oxide reduction of sulfide compounds
Code: BMS882
Gene: dsrS
Sequence: ACG ACG CGC TGT CCC CGG AAG ACG AGC TCC GCC TGC ACG TAC TCT TCA ACA CCG AGC TGC GGG CGG TTC GCA TCG ACG AGT CGA GCA TGA CCC TAT GGG C
SEQ ID NO: 232
Function: Oxide reduction of sulfide compounds
Code: BMS993
Gene: soxB
Sequence: ACC ACG CTC ACC GAG ATG ACG GGC GAA ACG ATC AAG ACG ATC ATG GAA GAC GTC GCC GAC AAT CTG TTC AAC GCC GAC CCG TAC TAC CAG CAG GGC GGC G

The result of hybridization with the biomining sample 1 and the microarray of this invention is shown. The result of hybridization between the second biomining sample and the microarray of the present invention is shown.

Claims (27)

An array of nucleotide sequences for the detection and identification of genes encoding proteins with biotechnology-related activity present in microbiological samples, representative of one, some or all of the following DNA fragments An array characterized in that it is attached to its surface:
a. At least one DNA fragment that specifically identifies a gene encoding a protein associated with biofilm formation;
b. At least one DNA fragment that specifically identifies a gene encoding a protein associated with CO ₂ fixation;
c. At least one DNA fragment that specifically identifies a gene encoding a protein associated with energy metabolism;
d. At least one DNA fragment that specifically identifies a gene encoding a protein associated with chemotaxis and motility;
e. At least one DNA fragment that specifically identifies a gene encoding a protein associated with iron oxidation;
f. At least one DNA fragment that specifically identifies a gene encoding a protein associated with nitrogen metabolism;
g. At least one DNA fragment that specifically identifies a gene encoding a protein associated with sulfur assimilation;
h. At least one DNA fragment that specifically identifies a gene encoding a protein associated with oxidation / reduction of sulfide compounds;
Here, hundreds of copies of each DNA fragment exist, form dots of a uniform composition, and are spatially distributed on the surface of the carrier.   The DNA fragment specifically identifying a gene encoding a protein related to biofilm formation is selected from the DNA fragments defined by the sequences of SEQ ID NOs: 1 to 86 and their reverse complement sequences The array of claim 1.   3. The DNA fragment according to claim 2, characterized in that the DNA fragment is complete or is within a large sequence whose specificity is conferred by the fragment or any subfragment contained therein. array.   4. An array according to claim 3, characterized in that said subfragments preferentially comprise 50 to 70 nucleotides. CO ₂ - wherein DNA fragments to identify specifically a gene encoding a protein associated with the stationary process, characterized in that it is selected from the DNA fragment defined by SEQ ID NO: 87-101 and reverse complementary sequences thereof The array of claim 1.   6. The DNA fragment according to claim 5, characterized in that the DNA fragment is complete or is within a large sequence whose specificity is given by the fragment or any subfragment contained therein. array.   The array of claim 6, wherein the subfragments comprise 50 to 70 nucleotides.   The array of claim 1, wherein the DNA fragment that specifically identifies a gene that encodes a protein associated with energy metabolism is selected from DNA fragments defined by SEQ ID NOs: 102-156 and their reverse complement sequences. .   9. The DNA fragment according to claim 8, characterized in that the DNA fragment is complete or is within a large sequence whose specificity is conferred by the fragment or any subfragment contained therein. array.   10. Array according to claim 9, characterized in that the subfragments preferentially comprise 50 to 70 nucleotides.   The DNA fragment specifically identifying a gene encoding a protein related to chemotaxis and motility is selected from DNA fragments defined by SEQ ID NOs: 157 to 192 and their reverse complement sequences The array of claim 1.   12. The DNA fragment according to claim 11, characterized in that the DNA fragment is complete, or is within a large sequence whose specificity is conferred by the fragment, or any subfragment contained therein. array.   13. Array according to claim 12, characterized in that the subfragments preferentially comprise 50 to 70 nucleotides.   The DNA fragment that specifically identifies a gene encoding a protein associated with iron oxidation is selected from DNA fragments defined by SEQ ID NOS: 193-197 and their reverse complement sequences. The array according to 1.   15. The DNA fragment according to claim 14, characterized in that the DNA fragment is complete or any subfragment that is within or contained within a large sequence given specificity by the fragment. array.   16. An array according to claim 15, wherein the subfragments preferentially comprise 50 to 70 nucleotides.   2. The DNA fragment that specifically identifies a gene encoding a protein associated with nitrogen metabolism is selected from DNA fragments defined by SEQ ID NOs: 198-203 and their reverse complement sequences. Array as described in   18. The DNA fragment according to claim 17, characterized in that the DNA fragment is complete or any subfragment that is within or contained within a large sequence of specificity conferred by the fragment. array.   19. An array according to claim 18, wherein the subfragments preferentially comprise 50 to 70 nucleotides.   The DNA fragment that specifically identifies a gene encoding a protein associated with sulfur assimilation is selected from DNA fragments defined by SEQ ID NOs: 204-210 and their reverse complement sequences. The array according to 1.   21. The DNA fragment according to claim 20, characterized in that the DNA fragment is complete or is any subfragment contained in or contained within a large sequence given specificity by the fragment. array.   The array of claim 21, wherein the subfragments preferentially comprise 50 to 70 nucleotides.   The DNA fragment that specifically identifies a gene encoding a protein related to the oxidation-reduction of a sulfide compound is selected from DNA fragments defined by SEQ ID NOs: 211 to 232 and their reverse complement sequences The array of claim 1.   24. The DNA fragment according to claim 23, characterized in that the DNA fragment is complete, or is within a large sequence whose specificity is conferred by the fragment, or any subfragment contained therein. array.   25. The array of claim 24, wherein the subfragments preferentially comprise 50-70 nucleotides. A method of detecting a gene encoding a protein having an activity related to biotechnology present in a microbiological sample, comprising:
a. Incubating a sample of labeled nucleic acid obtained from a microbiological sample on the array of claim 1;
b. After incubation, washing the array to remove unhybridized labeled DNA fragments;
c. Visualizing the dots of the array showing marks d. Verify that genes encoding proteins with activities related to the following functions are present in the sample: biofilm formation, CO ₂ fixation, energy metabolism, chemotaxis and motility, iron oxidation, Nitrogen metabolism, sulfur assimilation, and redox of sulfide compounds. 27. A method for identifying a gene encoding a protein having an activity related to biotechnology present in a microbiological sample, comprising incubating a sample of labeled nucleic acids on the array of claim 1 according to claim 26. If necessary, before
a. Extracting said nucleic acid from a microbiological sample; and / or b. A method comprising the step of fragmenting and marking a sample of nucleic acid with a labeled or sensitive nucleotide.

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