JP2008292313A - Knife for biological sample - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a knife for a biological sample which has a structure shaped so as to reduce the probability to injure a worker and is manufactured by a disposable material thermally disposed. <P>SOLUTION: The knife for cutting a gel containing a biological tissue or a soft biological tissue and preparing a biological sample piece comprises a vulcanized paper shaped sheet element having a side edge as a blade, and a grip section other than the blade. A product for wrapping the knife for the biological sample is shaped and wraps the knife so as to keep the knife in an aseptic condition. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a knife used for cutting a gel or biological soft tissue containing a biological tissue to create a biological specimen, and a method for producing a biological specimen using the knife.

Conventionally, steel knives, razors, cutter knives, and the like have been used as knives for cutting biological samples. Although these knives are sharp and convenient, the following problems have been pointed out.
That is, these knives are often thrown away to prevent contamination between samples, but in that case, the problem is that by making these durable steel knives disposable, resources are significantly wasted, In addition, steel blades are sharp and need to be disposed of with special care during disposal. Steel blades are nonflammable and cannot be incinerated, requiring landfill or special treatment. There is a problem that a worker may be injured by a sharp blade edge at the time of use or after use, or there is a risk of causing infection or poisoning due to injury by a contaminated blade.

  For workers who handle biological samples on a daily basis, the above-mentioned problems require a significant effort in addition to the work content, and are a burden on the work. Therefore, there is a need for a knife for producing a biological sample piece that is disposable and can be incinerated, but there is still no inexpensive knife that can meet such a demand.

By the way, a sheet of synthetic resin film, paper, metal foil or processed paper is wound and stored in a roll shape, and a durable incisor that is used while being pulled out to a predetermined length and cut. In the carton products with incisors provided, the conventional metal teeth have been devised and put on the market in place of the incisors made of vulcanized fibers, and the incisors made of vulcanized fibers have been put on the market. Is a sharp sawtooth incision for cutting a wrap film having a relatively large elongation rate such as polyethylene (for example, Patent Document 1 and Patent Document 2). When such a vulcanized paper sheet having sharp serrated incisors is used as the knife for producing the biological specimen, it becomes a burnable disposable knife. It is not preferable because the danger cannot be avoided.
Japanese Utility Model Publication No. 07-011527 JP 2002-105894 A Paper and Pulp Technology Association, “Paper and Paper Encyclopedia” Revised 5th edition, page 392 “Vulcanized paper” 4th edition issued on February 20, 2000

  Biological samples are relatively soft and easy to cut. In addition, some cutting knives need to be disposable to prevent contamination between samples, and in some cases, the samples may be dangerous to the human body. Need to be detoxified. Furthermore, even when disposable, if the sharpness is more than necessary, the container to be discarded may be broken and the worker may be injured. Therefore, it is also necessary to have a cutting edge shape that does not injure a person.

  The present invention eliminates the above-mentioned problems associated with conventional steel knives for biological specimen preparation, has a shape structure that does not hurt the operator, and is made of a disposable material that can be incinerated. An object of the present invention is to provide a knife for a biological sample.

  The present inventors pay attention to vulcanized paper having a relatively high rigidity and water resistance, and by providing a cutting edge function to the side edge portion of the living body, which is a relatively soft sample. It has been found that the present invention can be used as a knife for cutting a biological sample comprising a tissue-containing gel or a soft biological tissue. The present invention is based on an invention comprising a knife for biological samples formed from a vulcanized paper sheet, and is an invention selected from the inventions having the following technical matters.

(1) A knife for cutting a gel or biological soft tissue containing biological tissue to produce a biological sample piece, which is made of vulcanized paper having a side edge portion serving as a blade portion and a grip portion other than the blade portion. A knife for a biological sample comprising a sheet body.

(2) The blade portion is formed by projecting and fixing a vulcanized paper sheet that is thinner than the molded sheet body from a side edge portion at a partial side edge portion of the vulcanized paper molded sheet body. The knife for biological samples as described in (1) characterized by these.

(3) The blade part is formed by compressing a part of the side edge of the vulcanized paper molded sheet body so that the side edge is thinned into a wedge shape (1) or ( The biological sample knife as described in 2).

(4) The biological sample knife according to any one of (1) to (3), wherein the blade portion is formed at one side edge of a rectangular vulcanized paper-molded sheet body.

(5) Any of (1) to (4), wherein the formed vulcanized paper sheet has a thickness of the grip portion of 0.1 to 2.0 mm, preferably 0.5 to 2.0 mm. A knife for biological samples according to any one of the above.

(6) The biological sample knife according to (4) or (5), wherein the vulcanized paper molded sheet body has a length of the grip portion of at least 10 to 140 mm, preferably 50 to 100 mm.

(7) The biological sample knife according to any one of (1) to (6), wherein the vulcanized paper molded sheet body has a length of the blade portion of 10 to 100 mm, preferably 20 to 50 mm.

(8) The blade part has a thickness of at least a tip part of 0.1 to 2.0 mm, preferably 0.2 to 1.0 mm, according to any one of (1) to (7) Knife for biological samples.

(9) The knife for biological samples according to any one of (1) to (8), wherein corners of both end portions in the longitudinal direction of the blade portion are blunted.

(10) The gel according to any one of (1) to (9), wherein the gel containing the living tissue is a gel formed by solidifying a water-soluble polymer aqueous solution containing the living tissue. Biological sample knife.

(11) The biological sample knife according to (10), wherein the water-soluble polymer is a polyacrylamide polymer or an agarose polymer.

(12) A biological sample knife package product, wherein the biological sample knife according to any one of (1) to (11) is packaged in a sterile state.

(13) A method for producing a biological sample piece comprising cutting a biological tissue-containing gel or biological soft tissue using the biological sample knife according to any one of (1) to (11) above .

  According to the present invention, it is disposable, can be discarded / incinerated, and there is no risk of hand injuries, so no significant effort is required other than the work content, and the vulcanized can reduce the mental burden on the operator A knife comprising a paper molded sheet body is provided. In addition, a knife can be formed by cutting a vulcanized paper sheet into an arbitrary shape with scissors or the like. For example, a vulcanized paper sheet formed into a rectangular shape can be bent or curved, The operator can freely create a cut piece having an arbitrary shape and size from a scientific sample, which is almost impossible to use with a metal blade, and is very convenient.

Hereinafter, the knife for biological samples of the present invention, its packaged product, and its method of use will be further described.
The knife for biological samples of the present invention can be used to remove cut pieces for testing and testing from gel bodies and various living soft tissues formed by solidifying a water-soluble polymer aqueous solution containing biological tissues among biological samples. A knife used to make.

  The biological sample knife of the present invention is a molded sheet body made of vulcanized paper. As a result of studying various materials from the viewpoints of ease of use, environmental burden, production cost, etc., in order to produce a knife that meets the purpose of the present invention, the present inventors are most excellent those produced from vulcanized paper. I found out.

  Vulcanized paper is manufactured through a process such as washing, drying, pressing, and glossing of raw paper made from carefully selected chemical pulp and cotton fiber, treated with zinc chloride solution, and the structure is It is dense and excellent in rigidity and strength (Patent Document 2, Non-Patent Document 1). Since such vulcanized paper has high strength, if the thickness of the side edge of the sheet body is selected so that it can be easily used as a knife, the above gel body and biological soft tissue can be cut. It shows sufficient cutting ability as a blade that can be used. In addition, since it does not easily swell or soften even when it gets wet, sufficient strength is maintained even when it is used for cutting water-containing living tissue, and the renewable resource cellulose is Since it is a raw material, it has the advantage of less impact on the environment than other materials in recycling and disposal.

  Molded sheet made of vulcanized paper shows sufficient cutting ability as a blade that can cut the gel body and soft tissue above by adjusting the thickness of the side edge, but the sharpness of the side edge Is suitable for cutting the biological soft tissue and the like, and in particular, when handling biological samples, there is no possibility of penetrating a rubber glove that is regularly used by an operator, and there is no possibility of damaging the operator.

  The IF of the present invention comprising a molded sheet body made of vulcanized paper, when discarded after use, does not cause the plastic film waste storage bag to break through and contaminates the surrounding environment. Therefore, it can be discarded or recycled as ordinary combustible waste.

  Gel bodies are frequently used for various operations in the inspection, measurement and analysis of biological samples. Electrophoresis is widely used for the analysis of DNA, nucleic acids containing DNA, and proteins, but a gel is used for developing a sample by electrophoresis, and the sample after development is fixed as a gel depending on the purpose. It is cut out and used for inspection, analysis, etc.

  The gel body is formed by solidifying various water-soluble polymers dissolved in water. Examples of the substance for forming a gel used in the method for producing a biological sample piece of the present invention include polyacrylamide alone, a polyacrylamide synthetic polymer composed of a copolymer of acrylamide and other monomers, an agarose, etc. In general, water-soluble polymers derived from natural products are used.

  Examples of the biological soft tissue to which the knife for biological samples of the present invention is applied include soft and wet substances containing moisture excluding biological hard tissues such as bones, specifically, liver, adipose tissue, spleen, Not only those related to the human body such as the kidney, lungs, brain, fetus, embryo, but also soft tissues that are not dried or frozen, including animals (dogs and cats, insects, etc.) and plant tissues. .

  The knife made of a molded sheet body made of vulcanized paper of the present invention can be a knife that uses, as a blade portion, one side edge of the molded sheet body 1 punched into a specific shape from a vulcanized paper sheet. Further, as shown in FIG. 1a, it is also possible to form a blade portion 2 having improved cutting ability by compressing or grinding one side edge portion of the molded sheet body 1 to make it thin. FIG. 1B shows a blade 2 portion formed by joining a vulcanized paper sheet piece 3 having a thickness smaller than that of the molded sheet body to a part of the side edge of the molded sheet body serving as a blade portion of a knife. Reference numeral 4 denotes a portion that becomes a gripping portion.

  The thickness suitable for the knife of the present invention is 0.1 mm to 2.0 mm, more preferably 0.2 mm to 1.0 mm as a molded sheet body. If the thickness is less than 0.1 mm, the strength and rigidity are insufficient and the work is difficult to deform when cutting the sample, and it becomes difficult for the operator to hold the knife correctly. On the other hand, when the thickness is larger than 2.0 mm, the cutting ability of the edge portion is inferior, and the possibility that the cut surface of the sample becomes uneven is increased.

  The planar outer shape of the knife suitable for the present invention is generally a quadrangle formed of side 1, side 2, side 3, and side 4 as shown in FIG. 2, and the size of the knife has a hindrance to the biological sample cutting operation. Although there is no particular limitation as long as there is no limitation, generally, among the sides 1, 2, and 3 constituting the gripping portion, the preferable length of the side 1 is 20 mm to 150 mm, and the preferable length of the side 2 is 10 mm. 140 mm, the preferable length of the side 3 is 5 mm to 50 mm, and the preferable length of the side 4 serving as the blade portion is 10 mm to 100 mm.

  As an example of a preferable sheet processing method for manufacturing the knife of the present invention, a vulcanized paper sheet in which a blade portion 2 is formed by compressing or grinding one side edge as shown in FIG. There is a method of punching 1 into the shape shown in FIG. 3b to form a knife having the shape shown in FIG. According to such a punching method, a knife with a blade can be manufactured with high efficiency.

  Further, as shown in FIG. 4b, it is possible to perform a process of blunting the both end corners 5 of the blade part 2 of the knife of FIG. 4a formed in FIG. As a means for blunting the corner, a method of scraping off the corner or rounding at the time of punching is suitable. By applying such a process of blunting the corners, safety during disposal and work is further enhanced. Furthermore, the knives manufactured as described above are sterilized and then packaged in a packaging material capable of maintaining aseptic conditions, thereby further increasing the added value as a biological sample knife.

  EXAMPLES Hereinafter, although an Example demonstrates this invention concretely, this invention is not limited by a following example.

<Example 1>
A base paper having a CED viscosity of 7 cps was made by a paper machine from bleached kraft pulp made from cotton pulp, softwood and hardwood, and pulp made from non-wood. This base paper was treated with a solution having a zinc chloride concentration of 69.8Be and a temperature of 44 ° C., and 6 sheets were laminated and joined to produce a vulcanized paper sheet having a thickness of 0.3 mm.
This vulcanized paper is slit to a width of 70 mm to form a sheet, and a blade is formed on one side edge in the longitudinal direction of the sheet by grinding as shown in FIG. As shown, a 60 mm long molded sheet was punched from the paper sheet body to produce a knife having the shape shown in FIG.

<Reference example>
A white paperboard (basis weight: 270 g / mm ² , thickness: 0.3 mm) made from bleached kraft pulp was processed in the same manner as in Example 1 to produce a knife.

  Table 1 shows the results of using the knives produced in Example 1 and the reference example as a knife for cutting biological samples.

The knife using the vulcanized paper of Example 1 showed good cutting ability for both a relatively soft agarose gel body and a relatively hard polyacrylamide gel body.
On the other hand, the knife using the white paperboard of the reference example showed a good cutting ability for the agarose gel body, but for the polyacrylamide gel body, the blade softened with moisture when repeatedly used. The tendency to end up was seen.

  Moreover, the knife of Example 1 and the reference example did not penetrate even when the blade was pressed against a surgical rubber glove (Thoma New Surgical Glove manufactured by Utsunomiya Seisakusho). Furthermore, as shown in FIG. 4, the blunted blade edge did not penetrate even when the blade was pressed against a household wrap film (Mitsubishi Resin Dia Wrap).

  For comparison, the same operation was performed using a blade of a cutter knife often used for such operations. The cutting ability of the gel was good for both agarose gels and polyacrylamide gels, but it penetrated easily when a surgical rubber glove was pierced at the tip of the blade.

  The knife made of vulcanized paper of the present invention can be suitably used as a knife used for cutting biological samples, particularly gel bodies. Moreover, since it can cut | disconnect without damaging a wrap film even if it cuts a gel-like thing in the state which laid the wrap film for home use, it can utilize also as a disposable knife which does not injure a finger in a general household.

It is a figure which shows the shape of the knife for biological samples of this invention. It is a top view of an example of the knife for biological samples of the present invention. It is a figure which shows the manufacture example of the knife for biological samples of this invention. It is a figure which shows the example which raised the safety | security of the knife for biological samples of this invention.

Explanation of symbols

1: Sheet of vulcanized paper 2: Blade part 3: Sheet piece (blade part)
4: Grasping part 5: Blunt part

Claims (13)

  A knife for producing a biological sample piece by cutting a gel or biological soft tissue containing a biological tissue, from a vulcanized paper molded sheet body having a side edge part serving as a blade part and a grip part other than the blade part A biological sample knife characterized by comprising:   The blade portion is formed by fixing and arranging a vulcanized paper sheet that is thinner than the molded sheet body at a part of the side edge of the vulcanized paper molded sheet body. The biological sample knife according to claim 1.   The said blade part is formed by thinning the side edge part into a wedge shape by compressing a part of the side edge part of the molded sheet body made of vulcanized paper. Biological sample knife.   The biological blade knife according to any one of claims 1 to 3, wherein the blade portion is formed at one side edge of a rectangular vulcanized paper molded sheet body.   The biological sample knife according to any one of claims 1 to 4, wherein in the molded sheet body made of vulcanized paper, the grip portion has a thickness of 0.1 to 2.0 mm.   The biological sample knife according to claim 4 or 5, wherein the vulcanized paper molded sheet body has a length of the grip portion of at least 10 to 140 mm.   The biological sample knife according to any one of claims 1 to 6, wherein the vulcanized paper molded sheet body has a length of the blade portion of 10 to 100 mm.   The knife for biological samples according to any one of claims 1 to 7, wherein the blade portion has a thickness of at least a tip portion of 0.1 to 2.0 mm.   The biological sample knife according to any one of claims 1 to 8, wherein the blade has blunted corners at both ends in the longitudinal direction.   The biological sample knife according to any one of claims 1 to 9, wherein the gel containing biological tissue is a gel formed by solidifying a water-soluble polymer aqueous solution containing biological tissue. .   The biological sample knife according to claim 10, wherein the water-soluble polymer is a polyacrylamide polymer or an agarose polymer.   A biological sample knife package product, wherein the biological sample knife according to any one of claims 1 to 11 is packaged in a sterile state. A method for producing a biological sample piece comprising cutting a gel or biological soft tissue containing biological tissue using the biological sample knife according to any one of claims 1 to 11.