JP2008279238A - Regeneration treatment of arthropathy (heberden's nodositas), rheumatism, severe collagenosis marginal disease patient by gene therapy - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To solve issues that the cause of disease has high probability of genetic disposition and the mechanism of disease generation and individual difference cannot be eludicated at genetic level though the cause of disease can be explained by genome in genome and gene judgement, genome drug creation, etc. <P>SOLUTION: Though it seems to be not executed in orthopedics, cell-regenerated tissue, erythropoietin, a kind of hormone, etc. and gene vector HGF protein etc. autologously transplanted, a small amount of appropriate tissue or cell collected from a patient to culture and increase the cell. structure of the tissue made according to circumstances, tissue regeneration by somatic stem cell, somatic stem cell separately collected from cells, and so forth are applied. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

Regenerative medicine is a treatment method that regenerates the function of organs that have become dysfunctional or tissue that has been lost due to any cause, or promotes the regeneration of organs or tissues, and performs bioactive injection to help the body regenerate itself. is.
Broadening the interpretation of regenerative medicine includes the treatment of anemia with erythropoietin injection and the treatment of leukopenia with granular colony stimulating factor.

In 1995, gene therapy was implemented for ADA deficiency and several types of cancer.

Regenerative treatment with gene therapy has not yet been implemented in the orthopedic field.
In order to cure the skin that has been lost with a wide range of hot water, there is a treatment that uses "regenerated skin" that has been cultivated and increased to form a sheet.

In order to cure the cornea lost by corneal ulcers, there are treatments that cultivate mucosal cells in the mouth, increase the number of ulcers by regenerating epithelium, and so on.
The composition of DNA (gene) is a sequence of three bases. The DNA that makes up a chromosome is a combination of two molecules in a spiral. The structure of the molecule is a columnar structure formed by connecting sugars and phosphoric acid called deoxyribose. One helix has a diameter of 2 nm (1 nm is 1 / 1,000,000 mm). It is. Inside the helix, four types of bases (adenine, guanine, cytonin, thymine) are combined and arranged like a spiral staircase. The bases are paired with each other, adenine binds thymine and guanine binds to cytonin, and other combinations do not. Genetic information is transmitted only by the arrangement of these four types of bases. Protein synthesis is based on a sequence of three bases, which is called a codon. Twenty kinds of amino acids are determined by codons, and amino acids are combined to form various proteins. Approximately 60 trillion cells of the human body are created, and each person is acting in a different form and shape. Gene therapy is expected by human genome analysis. The total number of human genome and mouse genes is 23,000, and flies are 1 to 20,000, which is close to human DNA. What can be understood from the human genome analysis is all genetic information of human chromosomes. The Human Genome Project unveiled the entire genetic information of human chromosomes, that is, the outline of all DNA base sequence information in 2003, and was completely decoded in the fall of 2004. What is revealed here is the sequence of all bases. That alone does not reveal the function of genes and nucleotide sequences. The next genome research goal is to clarify the significance of the human genome sequence information. Its purpose is to determine how the nucleotide sequence is related to its function and, as a result of the interaction of the genes, the cell function, its relationship with disease development, and the diversity that affects individual differences in gene expression and function. It is to clarify. How does the genome react to environmental changes? What genes are expressed? Which proteins interact with each other. A new way of understanding and interpreting the significance of genetic information, etc. has been born in the name of networks and circuits (overall interaction).

It is positioned as an important pillar in human progress in the 21st century.
Biotechnology, biotechnology-related trends, genome and gene diagnosis, genome drug discovery, and other biotechnology headings are prominent. The cause of the disease is being explained by genes.
Adult diseases such as diabetes, hyperlipidemia, and hypertension, or lifestyle-related diseases are more likely to occur in the family if they occur frequently in the family, and the cause may be a genetic predisposition to the disease. I came.
There are various cases such as heart disease, cerebral infarction, infectious disease, malignant tumor (cancer).
Today, the causes of many of these diseases are more or less thought to be related to genes.
Advances in gene analysis technology have made it possible to elucidate the mechanisms of various diseases and individual differences at the genetic level.
Genetic testing detects the causative genes of these diseases, so that early treatment of the diseases and the selection of appropriate treatments can help prevent effective treatment and even worsening of the disease.
These efforts are accelerating because the complete genetic sequence information (genome) of the human chromosome was completed in 2003.
There is a limit to the information that can be obtained by genetic testing, and misuse can not only waste expensive testing costs but also provide harmful information.
Appropriate knowledge and understanding of genetic testing is required.

It would be necessary to conduct a lot of genetic testing with mice and gain knowledge of each disease.
Since arthropathy (Heberden tuberculosis), rheumatism, and collagen disease are genetic diseases, it is better to treat them as genetic diseases and to perform regenerative treatment for them.

I think that the worn cartilage part that is lost for some reason (immune dysfunction) will be regenerated by regenerative treatment.

To raise the level of orthopedics and to improve genetic research as a measure against genetic diseases.
The Ministry of Health and Welfare needs to review genetic diseases.

There is no working example due to lack of recognition of genetic disease.

We believe that implementing the Human Genome Project will bring significant benefits to the industry.

Illustration of regenerative medicine administered by local injection of cells / regenerative tissues or physiologically active substances or genes that act on regeneration Structure diagram of gene (DNA) and sequence diagram of three bases

Explanation of symbols

A Adenine T Thymine G Guanine C Cytonin

Claims (1)

It is reproductive medicine by gene therapy for regenerative treatment with arthropathy (Heberden tuberculosis) rheumatism / collagen disease related diseases.
Like cancer, arthropathy, rheumatism, and collagen disease are thought to be mainly due to a decline in immune function.
Although it has not been applied in practice in the field of orthopedics, erythropoietin, a kind of hormone, is injected into a patient, and cells and regenerated tissue are transplanted by self-transplantation by cell culture, tissue regeneration, tissue by somatic dry cells Regeneration, tissue regeneration by embryonic dry cells (ES cells), that is, administration of cell tissues or physiologically active substances and genes that also work by local injection.
In short, I think that it is possible to regenerate the worn cartilage part for people with arthropathy, rheumatism, and collagen disease.

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