JP2008237210A5 - - Google Patents

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Publication number
JP2008237210A5
JP2008237210A5 JP2007293325A JP2007293325A JP2008237210A5 JP 2008237210 A5 JP2008237210 A5 JP 2008237210A5 JP 2007293325 A JP2007293325 A JP 2007293325A JP 2007293325 A JP2007293325 A JP 2007293325A JP 2008237210 A5 JP2008237210 A5 JP 2008237210A5
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Japan
Prior art keywords
modified fadgdh
substituted
fadgdh
modified
glucose
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JP2007293325A
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Japanese (ja)
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JP2008237210A (en
JP4348563B2 (en
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Priority to JP2007293325A priority Critical patent/JP4348563B2/en
Priority claimed from JP2007293325A external-priority patent/JP4348563B2/en
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Claims (8)

野生型のアスペルギルス・オリゼ由来のフラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼ（ＦＡＤＧＤＨ）よりも熱安定性が向上した改変型ＦＡＤＧＤＨであって、（１）配列番号２のアミノ酸配列において、１６３位のグリシンがアルギニンに置換され、かつ、５５１位のバリンがシステインに置換されているか、または、（２）配列番号２のアミノ酸配列において、１６３位のグリシンがリジンに置換され、かつ、５５１位のバリンがシステインに置換されている、熱安定性が向上した改変型ＦＡＤＧＤＨ。 A modified FADGDH having improved thermostability over a flavin adenine dinucleotide-dependent glucose dehydrogenase (FADGDH) derived from wild-type Aspergillus oryzae, (1) In the amino acid sequence of SEQ ID NO: 2, glycine at position 163 is Arginine is substituted, and valine at position 551 is substituted with cysteine, or (2) in the amino acid sequence of SEQ ID NO: 2, glycine at position 163 is substituted with lysine, and valine at position 551 is cysteine modified FADGDH substituted, thermal stability is improved to. 請求項１に記載の改変型ＦＡＤＧＤＨをコードする遺伝子。 A gene encoding the modified FADGDH according to claim 1 . 請求項２に記載の遺伝子を含むベクター。 A vector comprising the gene according to claim 2 . 請求項３に記載のベクターで形質転換された形質転換体。 A transformant transformed with the vector according to claim 3 . 請求項４に記載の形質転換体を培養することを特徴とする改変型ＦＡＤＧＤＨの製造方法。 A method for producing a modified FADGDH, wherein the transformant according to claim 4 is cultured. 請求項１に記載の改変型ＦＡＤＧＤＨを含むグルコースアッセイキット。 A glucose assay kit comprising the modified FADGDH according to claim 1 . 請求項１に記載の改変型ＦＡＤＧＤＨを含むグルコースセンサー。 A glucose sensor comprising the modified FADGDH according to claim 1 . 請求項１に記載の改変型ＦＡＤＧＤＨを含むグルコース測定法。 A glucose measurement method comprising the modified FADGDH according to claim 1 .
JP2007293325A 2006-11-14 2007-11-12 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase Active JP4348563B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007293325A JP4348563B2 (en) 2006-11-14 2007-11-12 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP2006308337 2006-11-14
JP2006336351 2006-11-14
JP2007035980 2007-02-16
JP2007035978 2007-02-16
JP2007035979 2007-02-16
JP2007045372 2007-02-26
JP2007293325A JP4348563B2 (en) 2006-11-14 2007-11-12 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase

Related Child Applications (3)

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JP2009006900A Division JP5282581B2 (en) 2006-11-14 2009-01-15 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009126142A Division JP5169991B2 (en) 2006-11-14 2009-05-26 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009126143A Division JP2009225801A (en) 2006-11-14 2009-05-26 Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase

Publications (3)

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JP2008237210A JP2008237210A (en) 2008-10-09
JP2008237210A5 true JP2008237210A5 (en) 2009-03-05
JP4348563B2 JP4348563B2 (en) 2009-10-21

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Family Applications (4)

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JP2007293325A Active JP4348563B2 (en) 2006-11-14 2007-11-12 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009006900A Active JP5282581B2 (en) 2006-11-14 2009-01-15 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009126142A Active JP5169991B2 (en) 2006-11-14 2009-05-26 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009126143A Withdrawn JP2009225801A (en) 2006-11-14 2009-05-26 Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase

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JP2009006900A Active JP5282581B2 (en) 2006-11-14 2009-01-15 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009126142A Active JP5169991B2 (en) 2006-11-14 2009-05-26 Modified flavin adenine dinucleotide-dependent glucose dehydrogenase
JP2009126143A Withdrawn JP2009225801A (en) 2006-11-14 2009-05-26 Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase

Country Status (4)

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JP (4) JP4348563B2 (en)
CN (1) CN101535476B (en)
AT (1) ATE496124T1 (en)
DE (1) DE602007012130D1 (en)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2022850T3 (en) * 2006-05-29 2011-12-19 Amano Enzyme Inc Flavin-adenine dinucleotide-binding glucose dehydrogenase
JP5398004B2 (en) * 2007-12-28 2014-01-29 池田食研株式会社 Modified glucose dehydrogenase gene
JP4648993B2 (en) 2009-06-04 2011-03-09 キッコーマン株式会社 Flavin-binding glucose dehydrogenase
WO2011034108A1 (en) 2009-09-16 2011-03-24 東洋紡績株式会社 Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase
JP5803081B2 (en) * 2009-10-09 2015-11-04 東洋紡株式会社 Method for improving temperature dependence of FAD dinucleotide-dependent glucose dehydrogenase
JP5850291B2 (en) * 2009-11-06 2016-02-03 東洋紡株式会社 A method to improve the temperature dependence of flavin adenine dinucleotide-dependent glucose dehydrogenase
JP5660271B2 (en) * 2009-11-13 2015-01-28 東洋紡株式会社 Glucose dehydrogenase and method for producing the same
CN103981158A (en) 2009-12-05 2014-08-13 天野酶株式会社 Mutant enzyme and application thereof
JP5899616B2 (en) * 2009-12-28 2016-04-06 東洋紡株式会社 A method to improve the temperature dependence of flavin adenine dinucleotide-dependent glucose dehydrogenase
EP2589655A4 (en) 2010-06-29 2013-12-04 Ultizyme Int Ltd Glucose dehydrogenase
JP2012029677A (en) * 2010-07-08 2012-02-16 Toyobo Co Ltd Method for modifying substrate specificity of flavin adenine dinucleotide-dependent glucose dehydrogenase
JP5811521B2 (en) * 2010-09-09 2015-11-11 東洋紡株式会社 Method for improving the specific activity of flavin adenine dinucleotide-dependent glucose dehydrogenase
WO2012073987A1 (en) 2010-12-01 2012-06-07 キッコーマン株式会社 E. coli transformant, production method for flavin-bound glucose dehydrogenase using same, and mutant flavin-bound glucose dehydrogenase
JP5969392B2 (en) 2010-12-02 2016-08-17 キッコーマン株式会社 Flavin-binding glucose dehydrogenase, method for producing flavin-binding glucose dehydrogenase, and yeast transformant used therefor
WO2012169512A1 (en) * 2011-06-07 2012-12-13 キッコーマン株式会社 Flavin-binding glucose dehydrogenase, method for manufacturing flavin-binding glucose dehydrogenase, and glucose measurement method using same
JP6079038B2 (en) 2011-08-11 2017-02-15 東洋紡株式会社 Novel glucose dehydrogenase
JP5584740B2 (en) * 2011-09-26 2014-09-03 アークレイ株式会社 Glucose sensor
JP6212852B2 (en) 2011-10-31 2017-10-18 東洋紡株式会社 Novel glucose dehydrogenase
US9493814B2 (en) 2011-11-02 2016-11-15 Kikkoman Corporation Flavin-binding glucose dehydrogenase having improved substrate specificity
WO2013118798A1 (en) 2012-02-09 2013-08-15 東洋紡株式会社 Novel glucose dehydrogenase
JP6258298B2 (en) * 2012-05-03 2018-01-10 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト Glycosylated modified flavin adenine dinucleotide-dependent glucose dehydrogenase
WO2014002973A1 (en) 2012-06-29 2014-01-03 東洋紡株式会社 Novel glucose dehydrogenase
US9796963B2 (en) 2012-09-10 2017-10-24 Toyobo Co., Ltd. Glucose dehydrogenase
GB201223166D0 (en) * 2012-12-21 2013-02-06 Alere Switzerland Gmbh Test strip
JP6493212B2 (en) 2013-10-21 2019-04-03 東洋紡株式会社 Novel glucose dehydrogenase
JP6311270B2 (en) * 2013-10-29 2018-04-18 東洋紡株式会社 Flavin adenine dinucleotide-dependent glucose dehydrogenase with excellent thermostability
WO2015099112A1 (en) * 2013-12-27 2015-07-02 キッコーマン株式会社 Flavin-binding glucose dehydrogenase exhibiting improved heat stability
JP6826315B2 (en) 2015-01-16 2021-02-03 東洋紡株式会社 FAD-dependent glucose dehydrogenase
WO2016163448A1 (en) 2015-04-09 2016-10-13 東洋紡株式会社 Enzyme preparation for use in measurement of glucose
JP7084867B2 (en) 2016-05-09 2022-06-15 キッコーマン株式会社 Flavin-bound glucose dehydrogenase variant
JP6390776B2 (en) * 2017-09-15 2018-09-19 東洋紡株式会社 Flavin adenine dinucleotide-dependent glucose dehydrogenase with excellent thermostability
WO2021210282A1 (en) * 2020-04-17 2021-10-21 東洋紡株式会社 Mutant flavin adenine dinucleotide-dependent glucose dehydrogenase and method for producing same
CN113393900B (en) * 2021-06-09 2022-08-02 吉林大学 RNA state inference research method based on improved Transformer model

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000350588A (en) * 1999-04-08 2000-12-19 Koji Hayade Glucose dehydrogenase
JP2004344145A (en) * 2002-05-27 2004-12-09 Toyobo Co Ltd Pyrroloquinoline quinone (pqq) dependent modified glucosedehydrogenase substance excellent in substrate specificity and stability
CN100415877C (en) * 2002-12-24 2008-09-03 池田食研株式会社 Coenzyme-binding glucose dehydrogenase
WO2005045016A2 (en) * 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides
JP4639302B2 (en) * 2004-04-23 2011-02-23 アークレイ株式会社 Mutant glucose dehydrogenase
EP1862543B1 (en) * 2005-03-25 2013-04-03 Ikeda Food Research Co. Ltd. Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same
WO2006109578A1 (en) * 2005-04-05 2006-10-19 Amano Enzyme Inc. Altered pyrroloquinoline-quinone-dependent glucose dehydrogenase and method of improving substrate specificity of pyrroloquinoline-quinone-dependent glucose dehydrogenase

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