JP2008143861A - Alcohol metabolism promoter - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new alcohol metabolism promoter promoting alcohol metabolism through a path of oxidation by an enzyme in gastric mucosa, and having the contribution of such a mechanism as to be neither alcohol metabolism promotion by hepatic function advance nor alcohol absorption hindrance via digestive tracts. <P>SOLUTION: The new alcohol metabolism promoter or an aldehyde dehydrogenase expression promoter, comprises, as an active ingredient, α-lactalbumin (a globular protein accounting for about 25 wt.% of whey protein and having a molecular weight of about 14,100). <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an alcohol metabolism promoter containing α-lactalbumin as an active ingredient.

  Alcohol drink intake is said to have a good physical and mental effect if it is in an appropriate amount, but alcohol also has a peculiar preference. There may be symptoms of poisoning.

  In order to prevent or alleviate such unfavorable symptoms due to alcohol intake, conventionally, drugs or foods or drinks that suppress an increase in blood alcohol concentration after alcohol intake have been proposed. For example, an alcohol absorption inhibitor (Patent Document 1: Japanese Patent Application Laid-Open No. 2005-289933) containing one or more selected from the group consisting of lactulose, maltitol and lactitol as an active ingredient, and blood containing glycerol as an active ingredient Alcohol concentration-inhibiting agent (Patent Document 2: JP-A-6-14751), alcohol absorption inhibitor characterized by containing at least one glycoside having saponigen as oleanolic acid, presenegenin, hederagenin and protoesigenin (Patent Document 3: Japanese Patent Laid-Open No. 7-53385), an alcohol absorption inhibitor (Patent Document 4: Japanese Patent Laid-Open No. 8-277221) containing caffeine as an active ingredient, a herbal powder selected from One or more of extract and carnitine chloride Alcohol absorption inhibitor as an active ingredient (Patent Document 5: Japanese Patent No. 2891738), alcohol absorption inhibitor as an active ingredient glycyl-glycine: (Patent Document 6 Japanese Patent No. 2905231) is.

α-Lactalbumin is a globular protein with a molecular weight of about 14,100 that occupies about 25% of whey protein (weight, hereinafter the same unless otherwise specified), and is known to be a protein involved in lactose synthesis. (Non-patent Document 1: Kunio Yamauchi, edited by Kenkichi Yokoyama, “Milk General Encyclopedia”, Asakura Shoten Co., Ltd., page 35, 1992). In addition, α-lactalbumin has gelling properties and is known to be used in foods as a masking effect and quality improver in addition to egg white substitutes and paste products (non-patent literature). 2: “Current Status and Future Prospects of '94 Protein & Peptide Foods”, Seed Planning, Inc., page 37, 1994). As for this α-lactalbumin, an anti-ulcer agent containing α-lactalbumin as an active ingredient (Patent Document 7: WO01 / 007077), a mucin production secretion promoter containing α-lactalbumin as an active ingredient (Patent Document) 8: JP-A-2002-255848), α-lactalbumin, and one or a mixture of two or more selected from the group consisting of pharmaceutically acceptable salts of α-lactalbumin as an active ingredient Gastrointestinal motility regulators (Patent Document 9: JP-A-2004-231643) and the like are known.
However, it is not known that α-lactalbumin has an alcohol metabolism promoting action, and it is not described in the literature.

JP 2005-289933 A Japanese Patent Laid-Open No. 6-14751 Japanese Unexamined Patent Publication No. 7-53385 JP-A-8-277221 Japanese Patent No. 2891738 Japanese Patent No. 2905231 WO01 / 007077 Publication JP 2002-255848 A JP 2004-231643 A Kunio Yamauchi, Kenkichi Yokoyama, “Milk General Encyclopedia”, Asakura Shoten Co., Ltd., page 35, 1992 “Current Status and Future Prospects of '94 Protein & Peptide Foods”, Seed Planning, Inc., page 37, 1994

  The above-mentioned drugs and foods and drinks that have been proposed in order to suppress the increase in blood alcohol concentration after alcohol intake will suppress the increase in blood alcohol concentration by enhancing liver function and promoting alcohol metabolism. The role can be broadly divided into those which are intended to suppress the increase in blood alcohol concentration by inhibiting the absorption of alcohol from the digestive tract.

  However, when alcohol metabolism in the liver is promoted by enhancing liver function, there is a big difference between the amount of alcohol ingested and the metabolic capacity of the liver. Even if the liver function is enhanced, it is not expected that all the alcohol absorbed from the digestive tract and carried to the liver will be instantly detoxified. Alcohol that has not been broken down by the liver goes around the whole body and returns to the liver to be broken down again, so that the action of alcohol through the blood reaches the whole body. The same applies to aldehydes which are alcohol metabolites. That is, from this viewpoint, it is desirable that alcohol is not taken into the blood from the digestive tract.

  In contrast, when the absorption of alcohol from the gastrointestinal tract is to be inhibited, the action of alcohol on the whole body via the blood and aldehydes are inhibited because the incorporation of alcohol from the gastrointestinal tract into the blood is inhibited. However, unabsorbed alcohol remains in the gastrointestinal tract and the gastrointestinal tract is exposed to the unabsorbed alcohol until it is metabolized or excreted. That is, even when absorption from the digestive tract is inhibited, it is desirable that the unabsorbed alcohol is actively metabolized.

  Therefore, there has been a demand for a novel alcohol metabolism promoter for solving such problems and suppressing an increase in blood alcohol concentration after alcohol intake.

  That is, an object of the present invention is to provide a novel alcohol metabolism promoter having a blood alcohol concentration increase suppressing effect or a blood alcohol concentration decrease promoting effect. It is another object of the present invention to provide a novel alcohol metabolism promoter having a contribution of a mechanism that neither promotes metabolism of alcohol by enhancing liver function nor inhibits absorption of alcohol from the digestive tract.

  The inventors focused on the fact that alcohol taken orally is metabolized mainly by two routes. One of the pathways is a pathway that is oxidized by enzymes in the gastric mucosa before being absorbed into the body, and the other is a pathway that is absorbed from the gastric mucosa and the small intestine and is mainly oxidized in the liver. And if alcohol metabolism through the pathway oxidized by enzymes of the gastric mucosa can be promoted, it contributes to a novel mechanism that contributes neither to the promotion of alcohol metabolism by enhancing liver function nor to the inhibition of alcohol absorption from the digestive tract. The idea was that an alcohol metabolism promoter could be provided.

  Based on this idea, we have been eagerly searching for substances that have such an action, and found that α-lactalbumin has the property of promoting alcohol metabolism through a pathway oxidized by enzymes in the gastric mucosa. . This alcohol metabolism promotion was carried out based on the promotion of aldehyde dehydrogenase expression in the gastric mucosa. The present invention has been completed based on these findings.

Accordingly, the present invention includes the following [1] and [2].
[1] An alcohol metabolism promoter containing α-lactalbumin as an active ingredient.
[2] An aldehyde dehydrogenase expression promoter containing α-lactalbumin as an active ingredient.
The present invention also includes the following [3] to [8].
[3] A blood alcohol concentration increase inhibitor comprising the alcohol metabolism promoter of [1].
[4] A blood alcohol concentration reduction promoter comprising the alcohol metabolism promoter of [1].
[5] An alcohol absorption inhibitor into blood, comprising the alcohol metabolism promoter of [1].
[6] An alcohol metabolism promoter in the gastric mucosa, comprising the alcohol metabolism promoter according to [1].
[7] An aldehyde dehydrogenase expression promoter in the gastric mucosa comprising the aldehyde dehydrogenase expression promoter according to [2].
[8] An alcohol metabolism promoter in the gastric mucosa comprising the aldehyde dehydrogenase expression promoter according to [2].

  That is, the alcohol metabolism promoter of the present invention, in a preferred embodiment thereof, is a blood alcohol concentration increase inhibitor, a blood alcohol concentration decrease accelerator, an alcohol absorption inhibitor into the blood, and / or alcohol metabolism in the gastric mucosa. It can be used as a promoter. In addition, the aldehyde dehydrogenase expression promoter of the present invention can be used as an aldehyde dehydrogenase expression promoter in the gastric mucosa and / or an alcohol metabolism promoter in the gastric mucosa in a preferred embodiment thereof.

  In accordance with the present invention, blood alcohol concentration increase inhibitor, blood alcohol concentration decrease promoter, blood alcohol absorption inhibitor, alcohol metabolism promoter, alcohol metabolism promoter in gastric mucosa, aldehyde dehydrogenase expression The promoter and the aldehyde dehydrogenase expression promoter in the gastric mucosa are collectively referred to as an alcohol metabolism promoter or the like (or alcohol metabolism promoter or the like) according to the present invention. Further, according to the present invention, blood alcohol concentration increase suppression, blood alcohol concentration decrease promotion, blood alcohol absorption suppression, alcohol metabolism promotion, gastric mucosal alcohol promotion, aldehyde dehydrogenase expression promotion, and The action of promoting the expression of aldehyde dehydrogenase in the gastric mucosa is collectively referred to as the action of promoting alcohol metabolism (or promoting alcohol metabolism) according to the present invention.

Furthermore, the present invention also includes the following [9] to [16].
[9] An alcohol metabolism promoter comprising α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier.
[10] A food and drink for promoting alcohol metabolism, comprising α-lactalbumin as an active ingredient.
[11] The food or drink according to [10], wherein the food or drink is in the form of a health food, a functional food, a special purpose food, a nutritional functional food, or a food for specified health use.
[12] Use of α-lactalbumin for producing an alcohol metabolism promoter.
[13] Use of α-lactalbumin for producing a food or drink for promoting alcohol metabolism.
[14] A food additive for promoting alcohol metabolism, comprising α-lactalbumin as an active ingredient.
[15] A feed for promoting alcohol metabolism, comprising α-lactalbumin as an active ingredient.
[16] A method for promoting alcohol metabolism of mammals by administering an alcohol metabolism promoter comprising α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier.

Furthermore, the present invention also includes the following [17] to [24].
[17] An aldehyde dehydrogenase expression promoter comprising α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier.
[18] A food and drink for promoting expression of aldehyde dehydrogenase, containing α-lactalbumin as an active ingredient.
[19] The food / beverage product according to [18], wherein the food / beverage product is in the form of a health food, a functional food, a special-purpose food, a functional nutrition food, or a food for specified health use.
[20] Use of α-lactalbumin for producing an aldehyde dehydrogenase expression promoter.
[21] Use of α-lactalbumin for producing a food or drink for promoting expression of aldehyde dehydrogenase.
[22] A food additive for promoting expression of aldehyde dehydrogenase, comprising α-lactalbumin as an active ingredient.
[23] A feed for promoting expression of aldehyde dehydrogenase, containing α-lactalbumin as an active ingredient.
[24] A method for promoting reduction of blood alcohol concentration in mammals by administering an aldehyde dehydrogenase expression promoter comprising α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier.

Furthermore, the present invention also includes the following [25] to [32].
[25] An alcohol metabolism promoter comprising α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier.
[26] A food and drink for promoting alcohol metabolism and the like, containing α-lactalbumin as an active ingredient.
[27] The food or drink according to [26], wherein the food or drink is in the form of a health food, a functional food, a special-purpose food, a nutrient functional food, or a food for specified health use.
[28] Use of α-lactalbumin for producing an alcohol metabolism promoter and the like.
[29] Use of α-lactalbumin for producing a food or drink for promoting alcohol metabolism.
[30] A food additive for promoting alcohol metabolism and the like, containing α-lactalbumin as an active ingredient.
[31] A feed for promoting alcohol metabolism, which contains α-lactalbumin as an active ingredient.
[32] A method for promoting alcohol metabolism in mammals by administering an alcohol metabolism promoter containing α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier.

  As the α-lactalbumin, α-lactalbumin isolated from a natural product can be used, or artificially synthesized α-lactalbumin can also be used. Preferably, α-lactalbumin isolated from natural products is used. In addition, α-lactalbumin is selected from the group consisting of α-lactalbumin, pharmaceutically acceptable solvates of α-lactalbumin, and pharmaceutically acceptable salts of α-lactalbumin. Alternatively, a mixture of two or more can be used.

  The present invention provides a novel alcohol metabolism promoter. The alcohol metabolism promoter of the present invention has a contribution of a mechanism that is neither the promotion of alcohol metabolism by the enhancement of liver function nor the inhibition of alcohol absorption from the digestive tract. That is, it has a contribution of a mechanism for promoting alcohol metabolism by a pathway oxidized by enzymes of the gastric mucosa.

  Therefore, in the alcohol metabolism promotion according to the present invention, alcohol is promoted to be metabolized before it is taken into the blood from the digestive tract, unlike when the liver function is simply enhanced to promote alcohol metabolism in the liver. Therefore, it is possible to reduce the load on the liver and at the same time reduce the effect of alcohol through the blood on the whole body. Similarly, aldehyde, which is a metabolite of alcohol, can also be reduced from reaching the whole body.

  Further, in the alcohol metabolism promotion according to the present invention, unlike the case where the absorption of alcohol from the digestive tract is simply inhibited, the metabolism of unabsorbed alcohol is promoted, so that unabsorbed alcohol remains in the digestive tract. As a result, the digestive tract can be kept from being exposed to unabsorbed alcohol.

  Furthermore, since the alcohol metabolism promotion according to the present invention is performed based on the promotion of the expression of aldehyde dehydrogenase in the gastric mucosa, the enhanced action of aldehyde dehydrogenase in the gastric mucosa causes the aldehyde, which is a toxic component, to enter the blood. Degradation is promoted without migrating, and it is possible to further reduce bad effects of drinking such as so-called sickness.

  Furthermore, since the alcohol metabolism promotion according to the present invention has the contribution of a unique mechanism as described above, the active ingredient and / or digestion of a metabolism promoter that enhances liver function and promotes alcohol metabolism in the liver. By combining with an active ingredient of an absorption inhibitor that inhibits the absorption of alcohol from the tube, a more powerful effect can be expected.

  The alcohol metabolism promoter and the like according to the present invention contain α-lactalbumin as an active ingredient. For this reason, the alcohol metabolism promoter according to the present invention is highly safe for humans and animals, and can be taken daily for a long time without worrying about side effects. Furthermore, since α-lactalbumin, which is an active ingredient of the present invention, has heat resistance, is water-soluble, and is stable even in an aqueous solution, the alcohol metabolism promoter according to the present invention is stable as a drug or food and drink. It is. Furthermore, since the alcohol metabolism promoter according to the present invention can be administered orally, it is simple and highly versatile. Furthermore, the alcohol metabolism promoter and the like according to the present invention can be produced stably and in large quantities from relatively inexpensive raw materials such as milk and whey as biomaterials. Furthermore, α-lactalbumin, which is an active ingredient of the present invention, has a good flavor as compared with conventionally known crude drugs, amino acids and the like, and thus can be taken orally together with luxury products. .

  Next, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the following preferred embodiments, and can be freely changed within the scope of the present invention. In the present specification, percentages are expressed by mass unless otherwise specified.

  The alcohol metabolism promoter and the like according to the present invention can be produced by containing α-lactalbumin as an active ingredient and a pharmaceutically acceptable carrier and formulating it by a known method. In the formulation, pharmaceutically acceptable excipients and other optional additives can be used. This formulation will be described in more detail below.

  As α-lactalbumin used as an active ingredient in the alcohol metabolism promoter and the like according to the present invention, those derived from humans or mammals can be suitably used. As amino acid sequences of these α-lactalbumins, EMBL-Accession No. P00709 (human-derived α-lactalbumin) registered in the database of European Molecular Biology Laboratory (EMBL), EMBL-Accession No. The amino acid sequence described in P00711 (bovine-derived α-lactalbumin) and the like can be exemplified.

  As α-lactalbumin used in the present invention, those derived from mammalian milk can be preferably used. When used for pharmaceutical compositions and foods and drinks for humans, α-lactalbumin derived from milk of cows, sheep, goats, etc., whose milk is traditionally used for food and drink is preferred, especially milk The thing of origin is preferable. This is because these have been used for human consumption during historical years, and thus have a very high level of safety for humans. In addition, whey derived from milk can be obtained stably and in large quantities as a by-product of dairy production, and thus is particularly suitable as a raw material for α-lactalbumin used in the present invention.

  Α-Lactalbumin derived from milk of mammals should be produced by the ammonium sulfate precipitation method in a conventional manner (for example, Kinjiro Yukawa, “Latest Revised Dairy Technology Handbook”, Dairy Technology Promotion Association, pages 120-122, 1975). In addition, the iron chloride method [Journal of Food Science, Vol. 50, pp. 1531 to 1536, 1985], ultrafiltration method (Japanese Patent Laid-Open No. 5-268879) Gazette), ion exchange method (Japanese Patent No. 2916047) and the like. More simply, commercially available α-lactalbumin (for example, Sigma, Davisco Foods, etc.) can be used. Furthermore, recombinant α-lactalbumin produced by microorganisms, animal cells, transgenic animals, etc. by genetic manipulation may be used.

  In the present invention, pharmaceutically acceptable salts of α-lactalbumin obtained by the above chemical or genetic engineering method can also be used, and examples include acid addition salts and carboxylates. can do. The pharmaceutically acceptable solvate of α-lactalbumin obtained by the above chemical or genetic engineering method can also be used. α-lactalbumin, pharmaceutically acceptable solvates of α-lactalbumin, and pharmaceutically acceptable salts of α-lactalbumin may be used alone or in combination of two or more. May be.

  As an active ingredient such as an alcohol metabolism promoter according to the present invention, an α-lactalbumin hydrolyzate obtained by hydrolyzing α-lactalbumin with a hydrolase can be used. In the present specification, α-lactalbumin hydrolyzate includes peptides derived from α-lactalbumin hydrolyzate.

  Using α-lactalbumin hydrolyzate in combination with the above-mentioned α-lactalbumin α-lactalbumin pharmaceutically acceptable solvate and / or pharmaceutically acceptable salts of α-lactalbumin Also good.

  The α-lactalbumin hydrolyzate can be produced, for example, by the method described later. Hereinafter, a method for hydrolyzing α-lactalbumin with a hydrolase will be specifically described.

  First, α-lactalbumin is dispersed and dissolved in water or hot water. As the raw material α-lactalbumin, α-lactalbumin derived from human or mammal is preferably used, and those listed above can be suitably used.

  The concentration of the α-lactalbumin solution thus obtained is not particularly limited, but it is usually desirable from the viewpoint of efficiency and operability to make the concentration range around 5 to 15% in terms of protein. Moreover, it is desirable from the viewpoint of preventing deterioration due to contamination with various bacteria that the α-lactalbumin solution is sterilized by heating at 70 to 90 ° C. for about 10 minutes to 15 seconds.

  Subsequently, it is preferable to add an alkali agent or an acid agent to the α-lactalbumin solution and adjust the pH of the solution to or near the optimum pH of the hydrolase used. The alkali agent or acid agent used here may be any alkali agent or acid agent as long as it is acceptable for foods or pharmaceuticals. Specifically, examples of the alkali agent include sodium hydroxide, potassium hydroxide, potassium carbonate and the like, and examples of the acid agent include hydrochloric acid, citric acid, phosphoric acid, acetic acid and the like.

  Next, a hydrolase solution is added to the α-lactalbumin solution. The hydrolase is not particularly limited as long as it is an enzyme that hydrolyzes a protein, and is derived from an animal (eg, trypsin, chymotrypsin, pepsin, pancreatin), a plant (eg, papain), or a microorganism (eg, lactic acid bacteria). Yeast, mold, Bacillus subtilis, actinomycetes, and the like) and peptidases are preferred, and endopeptidases are preferred. Among them, it is preferable to use pig-derived pepsin. One type of hydrolase may be used, or two or more types may be used in combination. When using 2 or more types of enzymes, each enzyme reaction may be performed simultaneously or separately.

  And the solution which added the hydrolase is hold | maintained at suitable temperature according to the kind of enzyme, for example, 30-60 degreeC, Desirably 45-55 degreeC, The hydrolysis of (alpha) -lactalbumin is started. The hydrolysis reaction time is a time to reach a preferable decomposition rate while monitoring the decomposition rate of the enzyme reaction. In the present invention, the decomposition rate is preferably 6 to 80%, and particularly preferably 10 to 70%. In order for the hydrolyzate of α-lactalbumin to exert actions such as alcohol metabolism promotion, it is preferable that at least the decomposition rate is not more than the upper limit of the above range. When the upper limit is exceeded, when the α-lactalbumin hydrolyzate is ingested by adding it to food or drink, there is a risk of causing a decrease in flavor. In addition, when the value is lower than the lower limit of the above range, the decomposition is hardly progressed, but it is considered that it is substantially difficult to perform the decomposition in a certain condition, so the decomposition rate is 6%. The above is preferable.

The method for calculating the degradation rate of the protein (here α-lactalbumin) in the present specification is a sample obtained by the Kjeldahl method (edited by the Japan Food Industry Association, “Food Analysis Method”, page 102, Korin Co., Ltd., 1984). The total nitrogen content of the sample was measured, and the formol-type nitrogen content of the sample was measured by the formol titration method (Mitada et al., “Food Engineering Experiments”, Volume 1, page 547, Yokendo, 1970). This is a method of calculating the decomposition rate from the value by the following equation.
Decomposition rate (%) = (formol nitrogen amount / total nitrogen amount) × 100

  The enzyme reaction is stopped by deactivation of the enzyme in the hydrolyzed solution, and can be performed by heat deactivation treatment by a conventional method. The heating temperature and holding time of the heat deactivation treatment are appropriately set under conditions that allow for sufficient deactivation in consideration of the thermal stability of the enzyme used. For example, it can be performed in a temperature range of 80 to 130 ° C. with a holding time of 30 minutes to 2 seconds.

  And it is preferable to adjust the pH of the reaction solution obtained after stopping the enzyme reaction to near neutrality by adding acid or alkali.

  The solution containing the α-lactalbumin hydrolyzate thus obtained can be used as it is, and if necessary, this solution can be used as a concentrated solution concentrated by a known method. Further, this concentrated solution can be used as a powder dried by a known method.

  The administration form of the alcohol metabolism promoter and the like according to the present invention can be administered before ingestion of alcohol, at the time of ingestion of alcohol, or after ingestion. Considering the mechanism of action according to the present invention, oral administration which is easy to reach the gastric mucosa and is a simple administration form is the most preferable administration form. However, not only oral administration but also various forms such as parenteral administration such as enteral administration can be applied. If the alcohol metabolism promoter of the present invention is administered before alcohol intake, particularly 30 minutes to 1 hour before, the increase in blood alcohol concentration after alcohol intake can be effectively suppressed from the initial stage. It is possible to enjoy the effects of alcohol metabolism promotion and the like according to the present invention to the maximum extent.

  In addition, the alcohol metabolism promoter of the present invention may be administered in an effective amount at once, or can be administered in several divided portions at intervals. In the case of administration in several divided doses, the total daily dose may be an effective dose.

  The content (effective amount) of α-lactalbumin in the alcohol metabolism promoter of the present invention is such that the total amount of α-lactalbumin contained in the alcohol metabolism promoter administered daily is 100 mg or more, Preferably, it is prepared to be 500 mg or more. Moreover, when the form which adds and ingests the alcohol metabolism promoter of this invention etc. to daily food-drinks is assumed, content (effective amount) of the alpha-lactalbumin contained in the alcohol metabolism promoter etc. of this invention is The content of α-lactalbumin contained in daily foods and drinks can be set as appropriate, and in this case, it is preferable to prepare at least 10 mg or more.

  Examples of alcohol metabolism promoters and the like (particularly sometimes referred to as oral alcohol metabolism promoters) applied to oral administration include tablets, capsules, granules, fine granules, powders and the like. Moreover, it is not limited to a solid state, and various liquids such as a suspension, an emulsion, a syrup, and an elixir can be used.

  Examples of alcohol metabolism promoters and the like (particularly described as parenteral alcohol metabolism promoters and the like) applied to parenteral administration include injections and suppositories.

  In the case of an oral alcohol metabolism promoter or the like, it can be produced according to a conventional method using excipients such as starch, lactose, sucrose, mannitol, carboxymethylcellulose, corn starch, and inorganic salts. In addition to the above excipients, binders, disintegrants, surfactants, lubricants, fluidity promoters, corrigents, flavoring agents, colorants, fragrances and the like can be included.

  Specifically, examples of the binder include starch, dextrin, gum arabic powder, gelatin, hydroxypropyl starch, methylcellulose, sodium carboxymethylcellulose, hydroxypropylcellulose, crystalline cellulose, ethylcellulose, polyvinylpyrrolidone, macrogol and the like.

  Examples of the disintegrant include starch, hydroxypropyl starch, carboxymethylcellulose sodium, carboxymethylcellulose calcium, carboxymethylcellulose, and low-substituted hydroxypropylcellulose.

  Examples of the surfactant include sodium lauryl sulfate, soybean lecithin, sucrose fatty acid ester, polysorbate 80 and the like.

  Examples of the lubricant include talc, waxes, hydrogenated vegetable oil, sucrose fatty acid ester, magnesium stearate, calcium stearate, aluminum stearate, polyethylene glycol and the like.

  Examples of the fluidity promoter include light anhydrous silicic acid, dry aluminum hydroxide gel, synthetic aluminum silicate, magnesium silicate and the like.

  The effects such as alcohol metabolism promotion according to the present invention are not only exhibited by the alcohol metabolism promoter produced as a pharmaceutical composition through the formulation as described above, but also contain α-lactalbumin as an active ingredient. It is also exhibited by food and drink, food additives and feed for promoting alcohol metabolism. By formulating as described above, it is also possible to produce foods and drinks, food additives and feeds for promoting alcohol metabolism. However, when it is set as the aspect of food-drinks, a food additive, and feed, it is preferable to manufacture as the following description.

  The food / beverage products for alcohol metabolism promotion etc. of this invention can be manufactured by containing (alpha) -lactalbumin, when manufacturing various food / beverage products. As alpha-lactalbumin, the thing similar to alpha-lactalbumin used for manufacture of the alcohol metabolism promoter mentioned above etc. can be used.

  The intake form of the food and drink for promoting alcohol metabolism of the present invention can be taken before ingestion of alcohol, at the time of ingestion of alcohol, or after ingestion. In addition, if the food / beverage products for alcohol metabolism promotion etc. of this invention are ingested before alcohol intake especially 30 minutes-1 hour ago, the raise of the blood alcohol concentration after alcohol intake will be effective effectively from an initial stage. It can be suppressed, and the effects such as alcohol metabolism promotion according to the present invention can be enjoyed to the maximum.

  Moreover, the food / beverage products for alcohol metabolism promotion etc. of this invention may take an effective amount at once, and can also take it divided into several times at intervals. When ingesting in several divided doses, the total daily amount should be an effective amount.

  The content (effective amount) of α-lactalbumin in the food or drink for promoting alcohol metabolism of the present invention is α-lactalbumin contained in the food or drink for promoting alcohol metabolism administered daily. It is preferable that the total amount of is adjusted to be 100 mg or more, preferably 500 mg or more. Moreover, when the form taken with a daily food / beverage product is assumed, the content (effective amount) of α-lactalbumin contained in the food / beverage product for promoting alcohol metabolism of the present invention is included in the daily food / beverage product. What is necessary is just to set suitably in view of content of (alpha) -lactalbumin, and it is preferable to prepare so that it may become at least 10 mg or more in this case.

  In the food and drink for promoting alcohol metabolism of the present invention, in addition to the above-mentioned α-lactalbumin, which is an active ingredient, for example, alcohol absorption-inhibiting saccharides such as lactulose, maltitol, and lactitol, and other Sugars such as dextrin and starch; proteins such as gelatin, soy protein and corn protein; amino acids such as alanine, glutamine and isoleucine; polysaccharides such as cellulose and gum arabic; fats and oils such as soybean oil and medium chain fatty acid triglyceride Can be contained.

  Specific examples of food and drink for promoting alcohol metabolism include beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid bacteria drinks (including concentrated concentrates and powders for preparation of these drinks); ice creams, Frozen desserts such as ice sherbet and shaved ice; noodles such as buckwheat, udon, harusame, gyoza skin, sukimai skin, chinese noodles, instant noodles; rice cakes, chewing gum, candy, gum, chocolate, tablet confectionery, snacks, biscuits, jelly, Sweets such as jam, cream, baked confectionery; processed fishery and livestock products such as kamaboko, ham, sausage; dairy products such as processed milk and fermented milk; prepared foods, breads; enteral nutrition food, food for specified health use, health Examples include functional foods such as supplements.

  The form of the functional food is preferably a granular, tablet or liquid supplement from the viewpoint of easily grasping the intake amount of the active ingredient.

  The food and drink for promoting alcohol metabolism of the present invention is used for promoting alcohol metabolism, for suppressing increase in blood alcohol concentration, for promoting decrease in blood alcohol concentration, for suppressing alcohol absorption into blood, or for alcohol metabolism in gastric mucosa. It is preferable to sell as a food / beverage product for which the use such as promotion is indicated, for example, “a food / beverage product displayed as an alcohol metabolism promotion product containing α-lactalbumin as an active ingredient”.

  The “display” act (display act) includes all acts for informing consumers of the use, and if it is a display that can recall and analogize the use, the purpose of the display Regardless of the contents of the display, the object / medium to be displayed, etc., all fall under the “display” act of the present invention. However, it is preferable to display in such an expression that the consumer can directly recognize the application. Specifically, the act of describing the above-mentioned use in the product or the product packaging relating to the food or drink of the present invention can be cited as a display act, and further, the product or product packaging describing the above-mentioned use is assigned, Display for distribution, transfer or delivery, importing, displaying advertisements on products, price lists or transaction documents for display or distribution, or listing the use for information Thus, an act provided by an electromagnetic (Internet etc.) method can be exemplified.

  On the other hand, the displayed content (display content) is a display approved by the government or the like (for example, a display that is approved based on various systems determined by the government and is performed in a mode based on such approval). It is preferable to attach such display contents to advertising materials at sales sites such as packaging, containers, catalogs, pamphlets, POPs, and other documents.

  Further, for example, indications as health food, functional food, enteral nutrition food, special purpose food, health functional food, food for specified health, functional nutrition food, quasi drug, etc. can be exemplified. In particular, a display approved by the Ministry of Health, Labor and Welfare, for example, a display approved by a food system for specific health use or a system similar thereto can be exemplified. Examples of the latter can include a display as a food for specified health use, a display as a condition specific food for specified health use, a display that affects the structure and function of the body, a display for reducing disease risk, etc. Speaking of the health promotion law enforcement regulations (April 30, 2003 Ministry of Health, Labor and Welfare Ordinance No. 86) labeling as food for specified health use (especially labeling the use of health), and similar The display can be listed as a typical example.

  The wording used to display the above uses is “for alcohol metabolism promotion”, “for suppression of increase in blood alcohol concentration”, “for promotion of decrease in blood alcohol concentration”, “alcohol absorption into blood” The present invention is not limited to the words “for suppressing” or “for promoting alcohol metabolism in the gastric mucosa”, and any other words can be used as long as they express the effects of promoting alcohol metabolism as described above. Needless to say, it is included in the range.

  The food additive for promoting alcohol metabolism and the like of the present invention can be produced by incorporating α-lactalbumin when producing various food additives. As alpha-lactalbumin, the thing similar to alpha-lactalbumin used for manufacture of the alcohol metabolism promoter mentioned above etc. can be used.

  In addition to α-lactalbumin as an active ingredient, food additives can contain additives such as commonly used excipients as desired. Further, other components known as food additives can be included as desired. There is no restriction | limiting in particular in the form of a food additive, It can take the usual form of a food additive, such as a powder, a granule, a tablet, a liquid.

  The food additive for promoting alcohol metabolism and the like of the present invention can be added to produce food and drink for promoting alcohol metabolism. Therefore, as long as it contains α-lactalbumin as an active ingredient and can be added to produce a food or drink for promoting alcohol metabolism, the food additive for promoting alcohol metabolism of the present invention is added. It is contained in the range of the agent.

  The feed for promoting alcohol metabolism of the present invention includes alcohol metabolism promotion, blood alcohol concentration increase suppression, blood alcohol concentration decrease promotion, blood alcohol absorption suppression, or alcohol in the gastric mucosa. It is preferable to sell it as a feed displaying the use such as for promoting metabolism, for example, “a feed containing α-lactalbumin as an active ingredient, which is displayed for promoting alcohol metabolism”.

  About the act of a display, it is as having mentioned above about food-drinks. The wording used to display the above uses is “for alcohol metabolism promotion”, “for suppression of increase in blood alcohol concentration”, “for promotion of decrease in blood alcohol concentration”, “alcohol absorption into blood” The present invention is not limited to the words “for suppressing” or “for promoting alcohol metabolism in the gastric mucosa”, and any other words can be used as long as they express the effects of promoting alcohol metabolism as described above. Needless to say, it is included in the range.

  The feed for promoting alcohol metabolism and the like of the present invention can be produced by incorporating α-lactalbumin as an active ingredient when various feeds are produced. As alpha-lactalbumin, the thing similar to alpha-lactalbumin used for manufacture of the alcohol metabolism promoter mentioned above etc. can be used.

  In the feed of the present invention, in addition to the active ingredient according to the present invention, for example, grains such as corn, wheat, barley, rye, and milo; vegetable oils such as soybean oil meal, rapeseed oil meal, coconut oil meal, and linseed oil meal; Rice bran such as bran, wheat straw, rice bran and defatted rice bran; Manufactured rice such as corn gluten meal and corn jam meal; Animal feed such as fish meal, skim milk powder, whey, yellow grease and tallow; Yeasts such as: mineral feeds such as tribasic calcium phosphate and calcium carbonate; fats and oils; simple amino acids; sugars and the like. Specific examples of feed include pet food, livestock feed, and fish feed.

  Although there is no restriction | limiting in particular in the form of feed, It is preferable at the point that it is easy to grasp | ascertain the intake of an active ingredient that it is a granular form, a tablet form, or a liquid supplement.

  The feed for promoting alcohol metabolism of the present invention includes alcohol metabolism promotion, blood alcohol concentration increase suppression, blood alcohol concentration decrease promotion, blood alcohol absorption suppression, or alcohol in the gastric mucosa. It is preferable to sell it as a feed displaying the use such as for promoting metabolism, for example, “a feed containing α-lactalbumin as an active ingredient, which is displayed for promoting alcohol metabolism”.

  About the act of a display, it is as having mentioned above about food-drinks. The wording used to display the above uses is “for alcohol metabolism promotion”, “for suppression of increase in blood alcohol concentration”, “for promotion of decrease in blood alcohol concentration”, “alcohol absorption into blood” The present invention is not limited to the words “for suppressing” or “for promoting alcohol metabolism in the gastric mucosa”, and any other words can be used as long as they express the effects of promoting alcohol metabolism as described above. Needless to say, it is included in the range.

  The alcohol metabolism promoter of the present invention, and the food and drink, food additive and feed for promoting alcohol metabolism, etc. are administered or ingested before ingestion of drinking alcohol. An increase in concentration can be effectively suppressed, and a decrease in blood alcohol concentration can be effectively promoted. This is thought to be due to the promotion of alcohol metabolism, particularly in the gastric mucosa, resulting in suppression of alcohol absorption into the blood from the digestive tract. This promotion of alcohol metabolism is considered to be due to the further promotion of aldehyde dehydrogenase expression, particularly in the gastric mucosa, by the active ingredient α-lactalbumin. Therefore, the alcohol metabolism promoter and the like of the present invention, and the foods and drinks, food additives and feeds for promoting alcohol metabolism are effective for prevention of wrinkles by alcohol consumption, promotion of sickness, prevention of alcoholism, etc. .

  As described above, the promotion of alcohol metabolism according to the present invention has a unique mechanism that α-lactalbumin, which is an active ingredient, exhibits an action such as promotion of alcohol metabolism in the gastric mucosa. Therefore, in addition to α-lactalbumin, which is an active ingredient, an active ingredient of a metabolic promoter that enhances liver function and promotes alcohol metabolism in the liver, and / or from the digestive tract, as conventionally known. A more powerful effect can be expected by combining with an active ingredient of an absorption inhibitor that attempts to inhibit the absorption of alcohol. That is, the alcohol metabolism promoter of the present invention, foods and drinks, food additives and feeds for alcohol metabolism promotion, etc., in addition to α-lactalbumin which is an active ingredient, alcohol function in the liver by enhancing liver function An active ingredient of a known alcohol metabolism promoter that promotes metabolism and / or an active ingredient of a known alcohol absorption inhibitor that inhibits the absorption of alcohol from the digestive tract can be included as desired. By this, it can be set as the alcohol metabolism promoter etc. which have a more powerful effect, and the food-drinks, food additive, and feed for alcohol metabolism promotion etc.

  For this purpose, known active ingredients that can be added in addition to α-lactalbumin include, for example, Patent Document 1: JP 2005-289933 A, Patent Document 2: JP 6-147551 A, Patent Document 3: JP-A-7-53385, Patent Document 4: JP-A-8-277221, Patent Document 5: Japanese Patent No. 2891738, Patent Document 6: Japanese Patent No. 2905231, etc. Specifically, one or more selected from the group consisting of lactulose, maltitol, and lactitol, one or more glycosides having saponigen as oleanolic acid, presenegenin, hederagenin, and protoecigenin, caffeine, chiku Any of herbal powder or extract selected from seth ginseng and kyoukyo, and carnitine chloride Mention may be made of the species or more, and glycyl-glycine.

Examples The present invention will be described in more detail with reference to the following test examples and examples, but the present invention is not limited to the following test examples and examples.

[Test Example 1]
[Method]
Test substance was orally administered to 11-week-old Wistar rats that had been fasted for 24 hours and water supply was stopped 3 hours before the start of test substance administration. After 30 minutes, 5 mL / kg of 60% ethanol whose gastric juice and pH were adjusted with 100 mM hydrochloric acid. Orally administered. Further, 60 minutes later, the entire stomach was removed, and the stomach tissue was dissolved and denatured with RNA-Bee (TEL-TEST). Total RNA was extracted from the lysate and purified by RNeasy (QUIAGEN). As the DNA chip, Affymetrix rat genome array 2.0 was used. A cRNA was prepared from 5 μg of purified RNA using an Affymetrix kit, and reacted with a DNA chip at 45 ° C. for 16 hours. After the reaction, washing was performed and reading was performed with a scanner. The obtained data was analyzed by Gene spring 7 (Silicon Genetics), and the gene expression of aldehyde dehydrogenases 1a4 and 2 was examined.
The test substances were water, α-LA (α-lactalbumin) (Davisco Foods), and OVA (ovalbumin) (Sigma). The dose of water was 5 mL / kg, and the doses of α-LA and OVA were 500 mg / kg per rat body weight. This was performed with 3 animals in each group, and the relative gene expression of each individual was compared.

[result]
The graph of a test result is shown in FIG.1 and FIG.2. Both the expression of aldehyde dehydrogenase 1a4 (FIG. 1) and the expression of aldehyde dehydrogenase 2 (FIG. 2) are highest in the group pre-administered with α-LA (α-lactalbumin), followed by OVA ( Ovalbumin) was the group pre-administered and the lowest in the group pre-administered with water.
That is, as a result of gene chip analysis of rat stomach tissue administered with alcohol, it was found that the expression of aldehyde dehydrogenase gene was promoted by the administration of α-LA (α-lactalbumin). This indicates that alcohol metabolism is promoted in rat stomach tissue.

[Test Example 2]
[Method]
Test substance was orally administered to 11-week-old Wistar rats that had been fasted for 24 hours and water supply was stopped 3 hours before the start of test substance administration. After 30 minutes, 5 mL / kg of 60% ethanol whose gastric juice and pH were adjusted with 100 mM hydrochloric acid. Orally administered. Blood was collected over time 15 to 240 minutes after ethanol administration, and the blood ethanol concentration was measured.
Water and α-LA were administered as test substances, and the dosage was 5 mL / kg of water and 200 mg / kg of α-LA rat body weight.

[result]
FIG. 3 shows a graph of the test results. From FIG. 3, it was confirmed that the ethanol concentration in blood was lower in the α-LA administration group than in the water administration group.
That is, as a result of experiments with rats administered with alcohol, it was found that the increase in blood alcohol concentration was suppressed by the administration of α-LA (α-lactalbumin). This indicates that promotion of alcohol metabolism occurs in the rat.

[Test Example 3]
[Method]
Twenty-four healthy men were divided into three groups of eight. Group 1 was ingested with water, group 2 with 100 g of an aqueous solution containing 0.5 g of α-LA, and group 3 with an aqueous solution containing 5 g of α-LA. Thirty minutes later, 60 mL of whiskey was drunk, blood was collected over time every hour, and blood alcohol concentration was measured.

[result]
FIG. 4 shows a graph of the test results. Table 1 is a table showing the test numerical values that are the basis of the graph of FIG. 4, and is a table of numerical data showing the promotion of the decrease in human blood ethanol concentration due to the intake of α-LA. From the graph of FIG. 4, in the α-LA intake group, a rapid decrease in blood alcohol concentration was observed compared to the water intake group. In addition, the blood alcohol concentration decreased more rapidly in the α-LA5g intake group than in the α-LA0.5g intake group.
That is, as a result of experiments in humans administered with alcohol, it was found that administration of α-LA (α-lactalbumin) promotes a decrease in blood alcohol concentration. This indicates that promotion of alcohol metabolism occurs in humans.

  The blood alcohol concentration is a value that reflects both the amount absorbed from the digestive tract and the amount processed in the liver. If a high blood alcohol concentration is maintained, it can be interpreted that the amount of alcohol absorbed from the digestive tract exceeds the amount processed in the liver, and a rapid decrease in blood alcohol concentration Can be interpreted as being less than the amount of liver processed.

  That is, the results of Test Examples 1 to 3 show that alcohol metabolism in the gastric mucosa was promoted by ingestion of α-LA, resulting in a decrease in alcohol absorbed into the blood from the gastrointestinal tract and suppression of an increase in blood alcohol concentration. It is also understood that the decrease in blood alcohol concentration was promoted.

  20 g of crystalline cellulose (manufactured by Wako Pure Chemical Industries, Ltd.) is collected in a 1000 ml capacity mortar (manufactured by Nakajima Seisakusho Co., Ltd.), mixed with 20 ml of water, and then sieved in advance with a 48-mesh sieve (manufactured by Wakanamori). 25 g of divided lactose (Megure) was added, and 55 g of α-lactalbumin (Davisco Foods) was added and mixed. The obtained wet mass is taken on a stainless steel 20 mesh sieve (manufactured by Wakashi Seisha Co., Ltd.) and extruded by hand onto a drying stainless steel plate to form granules, quickly and evenly distributed, put in a dryer, 25 Dry at 2 ° C. for 2 days to obtain fine granules. The granules are sieved with a polyethylene 20-mesh sieve (manufactured by Wakanamori), the granules that have passed through the sieve are spread on a wide paper, and 2 g of magnesium stearate (manufactured by Kanto Chemical Co., Inc.) that has been sieved with 48 meshes in advance is added. Add and mix by hand to homogeneity. Using a tableting machine (model KT-2, manufactured by Kimura Seisakusho Co., Ltd.), using an R punch with a diameter of 11 mm, the number of tablets is 10, the tablet weight is 0.5 g, and the Monsanto hardness is 9.5 to 11.5 kg. The compression pressure was set under the conditions, and tableting was performed to obtain 180 tablets containing about 50% α-lactalbumin. This has the effect of promoting alcohol metabolism.

  10.8 kg of whey protein enzyme degradation product (manufactured by Morinaga Milk Industry Co., Ltd.), 36 kg of dextrin (manufactured by Showa Sangyo Co., Ltd.), and a small amount of water-soluble vitamins and minerals were dissolved in 200 kg of water, and an aqueous phase was prepared in the tank. Separately, soybean salad oil (manufactured by Taiyo Yushi Co., Ltd.) 3 kg, palm oil (manufactured by Taiyo Yushi Co., Ltd.) 8.5 kg, safflower oil (manufactured by Taiyo Yufu Co., Ltd.) 2.5 kg, lecithin (manufactured by Ajinomoto Co., Inc.) 0.2 kg, An oil phase was prepared by mixing and dissolving 0.2 kg of a fatty acid monoglyceride (manufactured by Kao Corporation) and a small amount of a fat-soluble vitamin. The oil phase was added to the aqueous phase in the tank, stirred and mixed, then heated to 70 ° C., and further homogenized with a homogenizer at a pressure of 14.7 MPa. Next, the mixture was sterilized at 90 ° C. for 10 minutes, concentrated and spray-dried to prepare about 59 kg of intermediate product powder. To 50 kg of this intermediate product powder, 6.8 kg of sucrose (manufactured by Hokuren Co., Ltd.), 167 g of amino acid mixed powder (manufactured by Ajinomoto Co., Inc.), and 1.2 kg of α-lactalbumin (manufactured by Davisco Foods) are added and mixed uniformly. Approximately 57 kg of enteral nutritional powder containing α-lactalbumin was produced. This has the effect of promoting alcohol metabolism.

  90 g of skim milk powder (Morinaga Milk Industry Co., Ltd.) is dissolved in 800 ml of hot water at 50 ° C., 30 g of sugar (Nisshin Sugar Co., Ltd.), 14 g of instant coffee powder (Nestlé Co., Ltd.), 2 g of caramel (made by Showa Kako Co., Ltd.), and coffee Flavor (manufactured by Sanei Chemical Co., Ltd.) 0.01 g was sequentially added and dissolved while stirring, cooled to 10 ° C., 10 g of α-lactalbumin (manufactured by Davisco Foods) was added, and about 1% α-lactalbumin was contained. A milk drink was prepared. This has the effect of promoting alcohol metabolism.

A sports drink having the following composition was prepared by a conventional method.
α-Lactalbumin (Davisco Foods) 0.500 (mass%)
Sugar (manufactured by Nissin Sugar Co., Ltd.) 2.000
Fructose grape sugar solution (Nisshin Sugar Co., Ltd.) 4.000
Citric acid (manufactured by Saneigen FFI) 0.100
Sodium chloride (Kanto Chemical Co., Ltd.) 0.040
Vitamin C (manufactured by Saneigen FFI) 0.015
Potassium chloride (Kanto Chemical Co., Ltd.) 0.040
Water 93.305
This has the effect of promoting alcohol metabolism.

As a one-time composition, a powdered drink for sports having the following components was prepared by a conventional method.
α-Lactalbumin hydrolyzate (produced as described above) 2.0 (g)
Sun Sweet (Stevia / Saumatine Mixture: Saneigen FFI) 0.1
Aspartame (manufactured by Ajinomoto Co.) 0.06
Citric acid (manufactured by Saneigen FFI) 1.51
Malic acid (manufactured by Riken Chemical Co., Ltd.) 0.49
Gluconic acid (Fujisawa Pharmaceutical Co., Ltd.) 1.02
Citrus flavor (manufactured by Saneigen FFI) 0.3
Grapefruit flavor (manufactured by Saneigen FFI) 0.5
Soy lecithin (manufactured by Taiyo Chemical Co., Ltd.) 0.003
The sports powder drink was dissolved in 200 ml of water, and α-lactalbumin hydrolyzate was prepared as a sports drink containing about 1.0% by mass in terms of α-lactalbumin. It has the effect | action which accelerates | stimulates.

As a composition for one batch, a powdered drink for a racehorse having the following components was prepared by a conventional method.
α-Lactalbumin hydrolyzate (produced in the same manner as in Reference Example) 40.0 (g)
Lactose (Morinaga Milk Industry Co., Ltd.) 6.0
Citric acid (manufactured by Saneigen FFI) 5.0
Ascorbic acid (manufactured by San-Eigen FFI) 2.0
Dextrin (Matsuya Chemical Co., Ltd.) 8.0
In addition, the said powder drink for racehorses melt | dissolved in 2000 ml of tap water so that the final density | concentration of alpha-lactalbumin hydrolyzate might be about 2 mass% in conversion of alpha-lactalbumin, and prepared as a drink for racehorses.

FIG. 1 shows changes in the expression of aldehyde dehydrogenase 1a4 gene by pre-administration of α-LA. FIG. 2 shows changes in the expression of aldehyde dehydrogenase 2 gene by pre-administration of α-LA. FIG. 3 is a graph showing suppression of increase in blood alcohol concentration in rats by pre-administration of α-LA. FIG. 4 is a graph showing promotion of reduction in human blood ethanol concentration by ingestion of α-LA.

Claims (2)

  An alcohol metabolism promoter containing α-lactalbumin as an active ingredient.   An aldehyde dehydrogenase expression promoter containing α-lactalbumin as an active ingredient.