JP2008109867A5 - - Google Patents

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JP2008109867A5
JP2008109867A5 JP2006293750A JP2006293750A JP2008109867A5 JP 2008109867 A5 JP2008109867 A5 JP 2008109867A5 JP 2006293750 A JP2006293750 A JP 2006293750A JP 2006293750 A JP2006293750 A JP 2006293750A JP 2008109867 A5 JP2008109867 A5 JP 2008109867A5
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Prior art keywords
urate oxidase
amino acid
activity
action
oxidase
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JP2006293750A
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Japanese (ja)
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JP5207430B2 (en
JP2008109867A (en
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Priority claimed from JP2006293750A external-priority patent/JP5207430B2/en
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Publication of JP2008109867A5 publication Critical patent/JP2008109867A5/ja
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Claims (15)

下記特性を有する尿酸オキシダーゼ。
（１）酵素作用：酸素と水の存在下、尿酸に作用して、５−ヒドロキシイソウレア、過酸化水素、及び二酸化炭素を生成する作用を有する。
（２）阻害作用：ハロゲン化物イオン、硝酸イオン、及び／又はトリス緩衝液で（１）の作用が阻害されない。かつ、キサンチンで（１）の作用が阻害されない。
（３）至適ｐＨ：６．３〜７．８。
（４）至適温度：５５℃以上。
（５）作用適温の範囲：すくなくとも３０〜６０℃の範囲。
（６）安定性：安定化剤の非存在下、３０℃、１５分間で９８％以上の活性を保持する。 Urate oxidase having the following characteristics.
(1) Enzyme action: It acts on uric acid in the presence of oxygen and water to produce 5-hydroxyisourea, hydrogen peroxide, and carbon dioxide.
(2) Inhibitory action: The action of (1) is not inhibited by halide ions, nitrate ions and / or Tris buffer. And xanthine does not inhibit the action of (1).
(3) Optimal pH: 6.3 to 7.8.
(4) Optimal temperature: 55 ° C. or higher.
(5) Range of temperature suitable for action: At least 30 to 60 ° C.
(6) Stability: Retains 98% or more of activity at 30 ° C. for 15 minutes in the absence of a stabilizer. ６１０±２０ｎｍに吸収スペクトルが存在することを特徴とする、請求項１に記載の尿酸オキシダーゼ。 The urate oxidase according to claim 1, wherein an absorption spectrum exists at 610 ± 20 nm. Lysobacter sp. T-15株（受託番号ＦＥＲＭ Ｐ−２１０５６）由来であることを特徴とする、請求項１又は２に記載の尿酸オキシダーゼ。 The urate oxidase according to claim 1 or 2, characterized in that it is derived from Lysobacter sp. T-15 strain (Accession No. FERM P-21056). 下記（ａ）又は（ｂ）の何れかのアミノ酸配列からなる尿酸オキシダーゼ。
（ａ）配列番号２で表わされるアミノ酸配列；
（ｂ）配列番号２で表されるアミノ酸配列において、１又は数個のアミノ酸が欠失、置換もしくは付加されたアミノ酸配列であって、尿酸オキシダーゼ活性を有するアミノ酸配列： A urate oxidase comprising the amino acid sequence of either (a) or (b) below.
(A) the amino acid sequence represented by SEQ ID NO: 2;
(B) an amino acid sequence represented by SEQ ID NO: 2, one or several amino acids are deleted, substituted or added in the amino acid sequence, amino acid sequence having a urate oxidase activity: ラッカーゼ活性を併せ持つ、請求項１から４のいずれか１項に記載の尿酸オキシダーゼ。 The urate oxidase according to any one of claims 1 to 4, which also has laccase activity. ビリルビンオキシダーゼ活性を併せ持つ、請求項１から５のいずれか１項に記載の尿酸オキシダーゼ。 The urate oxidase according to any one of claims 1 to 5, which also has bilirubin oxidase activity. 請求項１から６のいずれか１項に記載の尿酸オキシダーゼを生産することを特徴とする微生物。 A microorganism characterized by producing the urate oxidase according to any one of claims 1 to 6. 受託番号ＦＥＲＭ Ｐ−２１０５６を有する、Lysobacter sp. T-15株。 Lysobacter sp. T-15 strain having accession number FERM P-21056. 以下の（ａ）または（ｂ）の塩基配列からなるポリヌクレオチド。
（ａ）配列番号１で表される塩基配列；
（ｂ）配列番号１で表される塩基配列において、１又は複数の塩基が欠失、置換もしくは付加された塩基配列であって、かつ、尿酸オキシダーゼ活性を有するタンパク質をコードする塩基配列： A polynucleotide comprising the following base sequence (a) or (b):
(A) the base sequence represented by SEQ ID NO: 1;
(B) a base sequence in which one or more bases are deleted, substituted or added in the base sequence represented by SEQ ID NO: 1 and which encodes a protein having urate oxidase activity: 請求項９に記載のポリヌクレオチドを含む組換えベクター。 A recombinant vector comprising the polynucleotide according to claim 9. 請求項１０に記載の組換えベクターを有する形質転換体。 A transformant comprising the recombinant vector according to claim 10. 請求項１１に記載の形質転換体を培地に培養し、培養物中に請求項１から６のいずれか1項に記載の尿酸オキシダーゼを生成蓄積させ、該培養物から該尿酸オキシダーゼを採取することを特徴とする尿酸オキシダーゼの製造方法。 A transformant according to claim 11 is cultured in a medium, the urate oxidase according to any one of claims 1 to 6 is produced and accumulated in the culture, and the urate oxidase is collected from the culture. A method for producing urate oxidase, characterized in that 請求項１から６のいずれか１項に記載の尿酸オキシダーゼを含有する組成物。 The composition containing the urate oxidase of any one of Claim 1 to 6. 請求項１から６のいずれか１項に記載の尿酸オキシダーゼ又は請求項１３に記載の組成物を少なくとも含む、尿酸測定キット。 A kit for measuring uric acid, comprising at least the uric acid oxidase according to any one of claims 1 to 6 or the composition according to claim 13. キサンチン、硝酸イオン、及びハロゲン化物イオンからなる群から選ばれる１以上の成分を含む試料において、請求項１から６のいずれか１項に記載の尿酸オキシダーゼ、請求項１３に記載の組成物、又は請求項１４に記載の尿酸測定キットを用いて尿酸を測定する方法。 A sample comprising one or more components selected from the group consisting of xanthine, nitrate ions, and halide ions, the urate oxidase according to any one of claims 1 to 6, the composition according to claim 13, or A method for measuring uric acid using the uric acid measurement kit according to claim 14.
JP2006293750A 2006-10-30 2006-10-30 NOVEL ENZYME AND COMPOSITION CONTAINING THE SAME Active JP5207430B2 (en)

Priority Applications (1)

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JP2008109867A JP2008109867A (en) 2008-05-15
JP2008109867A5 true JP2008109867A5 (en) 2009-11-12
JP5207430B2 JP5207430B2 (en) 2013-06-12

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5493935B2 (en) * 2010-02-03 2014-05-14 トヨタ自動車株式会社 Uricase inhibitor
US20170056439A1 (en) * 2015-08-25 2017-03-02 Oxy Young Co., Ltd. Oxygen-enriched water composition, biocompatible composition comprising the same, and methods of preparing and using the same
CN108888768A (en) * 2018-09-07 2018-11-27 大连大学 A kind of compound preparation of the urate oxidase containing marine low temperature

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