JP2008077061A - Adjustment method of guiding device for objective lens, adjustment device, and screen for adjustment - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To easily place the front end surface of a fine-diameter front end part, in an appropriate and satisfactory manner, the position with respect to an object of observation, in a state of the observation position not being fixed, and to simply and quickly acquire the image of the region of an object to be observed. <P>SOLUTION: An adjusting method for a guide device 3 which is mounted on the front end of an objective lens 2, being position adjustable in the optical axis direction and contacted with an observation object so as to make the focal position of the objective lens 2 coincide with an observation position, includes steps where in addition, a screen 1 for adjustment having a prescribed pattern is fixed to the front end surface of a guidance device 3; the guidance device 3, to which the screen 1 for adjustment is fixed, and the objective lens 2 are relatively moved in the optical axis direction; and then the relative position between the objective lens 2 and the guide device 3 is fixed, at a position where an image which is focused on the pattern of the screen 1 for adjustment is gained, or at a position at which the screen 1 and the guide device 3 are relatively moved farther by a prescribed distance from the position, and then the screen 1 for adjustment is removed from the guidance device 3. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an adjustment method, an adjustment device, and an adjustment screen for an objective lens guide device.

Conventionally, in order to eliminate the need for alignment work that has been performed each time when observing the inside of an experimental small animal or the like, an objective lens insertion tool that can be positioned by inserting an objective lens is used for the observation small experimental animal. A technique for fixing to the epidermis is known (for example, see Patent Document 1).
JP 2005-122947 A

  However, in the objective lens insertion tool of Patent Document 1, once the observation position in the body of the experimental small animal that is the observation target is once determined, the objective lens insertion tool is fixed to the observation target, so that the objective lens is repeatedly attached and detached. However, it is possible to position and observe at the same position, but in the process of searching for the observation position, it is necessary to adjust the focus position each time. In addition, when observing the surface of an organ or the like by inserting it into a living body into a thin tip provided at the tip of the objective lens, the tip surface of the thin tip is inserted into the living body and cannot be seen from the outside. There is a disadvantage that the positional relationship with the surface of the organ or the like cannot be accurately grasped, and the focal position must be aligned with the surface of the organ or the like by trial and error.

  The present invention has been made in view of the above-described circumstances, and easily disposes the distal end surface of the small-diameter distal end portion at an appropriate position with respect to the observation object in a state where the observation position is not fixed. It is an object of the present invention to provide an adjustment method, an adjustment device, and an adjustment screen for a guide device for an objective lens that can easily and quickly acquire an image of a site to be observed.

In order to achieve the above object, the present invention provides the following means.
The present invention is an adjustment method for a guide device, which is attached to the tip of an objective lens so that the position of the objective lens can be adjusted, and is brought into contact with an observation target so that the focal position of the objective lens coincides with the observation position. A guide device in which an adjustment screen having a predetermined pattern is fixed to a position where the optical axis of the objective lens is blocked when the guide device is attached to the front end surface of the device, and the adjustment screen is fixed And the objective lens are moved relative to each other in the direction of the optical axis at a position where an image in focus with the pattern of the adjustment screen is obtained, or a position where the image is further moved by a predetermined distance from the position. A method for adjusting the guide device is provided in which a relative position between the objective lens and the guide device is fixed and the adjustment screen is removed from the guide device.

  According to the present invention, when the guide device having the adjustment screen attached to the distal end surface is attached to the objective lens, the optical axis of the objective lens is blocked by the adjustment screen. Since the adjustment screen has a predetermined pattern, the objective lens is attached to the microscope for observation, and the objective lens and the guide device are moved relative to each other in the optical axis direction. An image in focus with the screen pattern is obtained. At this position, since the tip surface of the guide device coincides with the focal position of the objective lens, the objective lens and the guide device are fixed at this position or a position further moved by a predetermined distance from the position, and the adjustment screen is used. By removing the, the focal point can be accurately matched to the desired observation position when the tip surface of the guide device is brought into contact with the observation target.

In the above invention, the adjustment screen may be supported by the guide device, and the guide device and the objective lens may be moved relative to each other.
In this way, the adjustment screen can be moved relative to the objective lens by simply moving the guide device and the objective lens relative to each other without using other support members. The position can be adjusted.

Moreover, in the said invention, it is good also as moving the said guide apparatus and the said objective lens relatively, measuring the load concerning either the said guide apparatus or the said objective lens.
In this way, when the guide device and the objective lens are pressed against each other, the load can be measured, and the inconvenience of over-pressing can be prevented, and the guide device and the objective lens can be prevented from being damaged. it can.

Further, the present invention is an adjustment screen used in any of the above-described adjustment methods of the guide device, the screen main body having a flat surface that is brought into close contact with the tip of the guide device, and the screen main body being detachable from the guide device. And an attaching / detaching portion to be attached, and an adjustment screen having a predetermined pattern on the flat surface.
By doing so, it is possible to attach the flat surface of the screen body in close contact with the tip of the guide device by the operation of the attaching / detaching portion, and a clear image of a predetermined pattern provided on the flat surface can be obtained. By fixing the guide device and the objective lens at the position, the focus of the objective lens can be easily matched with a desired observation position with high accuracy.

In the above invention, the screen main body may be formed of a fluorescent material, and the pattern may be formed of a recess provided on a flat surface.
By doing so, the fluorescence generated in the fluorescent material is collected by the objective lens, and the pattern formed by the concave portion of the flat surface is acquired as a shadow image due to the influence of diffraction of the fluorescence and irregular reflection. By forming the pattern with the recesses, a fine pattern can be easily formed. The recess may be a flawed groove formed on a flat surface.

In the above invention, the screen body may be made of a fluorescent material, and the pattern may be printed on a flat surface.
Patterns can be easily formed by fine printing techniques such as screen printing.

Moreover, in the said invention, the said screen main body is comprised by the fluorescent material, and the said pattern is good also as being printed on the transparent sheet | seat which coat | covers a flat surface.
In this way, the screen body can be sterilized, disinfected, etc., and reused simply by replacing the sheet.

In the above invention, the screen main body may be made of a non-fluorescent material, and a pattern may be printed on the flat surface with the fluorescent material.
In this way, the focus position of the objective lens is accurately set at a desired observation position by fixing the guide device and the objective lens at a position where a clear image of fluorescence emitted from the pattern made of the fluorescent material is obtained. Can be matched. In addition, since the fluorescence is generated from the pattern, a clear image of the fluorescence can be obtained at the position where the fluorescence intensity reaches its peak, so the relative position between the guide device and the objective lens is automatically adjusted by measuring the amount of fluorescence. Can also be performed.

Moreover, in the said invention, it is good also as the said pattern being finely formed in the center part rather than the peripheral part.
By doing so, it is possible to observe a desired pattern by observing a large pattern at the periphery for a low-magnification objective lens and by observing a fine pattern at the center for a high-magnification objective lens. The focal position of the objective lens can be accurately matched with the position.

  Further, the present invention is an adjusting device for a guide device that is attached to the tip of an objective lens so that the position of the objective lens can be adjusted, and is brought into contact with an observation target so that the focal position of the objective lens coincides with the observation position. An objective lens fixing portion for fixing an objective lens to which a guide device having an adjustment screen having a predetermined pattern attached at a tip portion is attached so that the position of the guide device can be adjusted; a visible light source that illuminates the adjustment screen; There is provided an adjusting device for a guide device, comprising: an imaging optical system that forms an image of the pattern that has passed through the objective lens on the retina of an observer when a distal end surface coincides with a position corresponding to an observation position. .

  By doing so, the adjustment work of the guide device with respect to the objective lens can be performed without using the excitation light, and processing such as shielding of the excitation light during work is unnecessary, and the adjustment work can be performed more easily. Can do.

In the above invention, the relay optical system that forms an intermediate image of the pattern when the tip surface of the guide device coincides with the position corresponding to the observation position, and the intermediate image formed by the relay optical system. It is good also as providing the 2nd screen provided with the 2nd pattern arranged at a position.
The guide device is fixed to the objective lens at a position where an observation image in which the focus is simultaneously matched to both the pattern of the adjustment screen and the second pattern of the second screen is obtained, or a position moved from the position by a predetermined distance. By doing so, the position of the guide device can be adjusted with higher accuracy.

Further, the present invention is an adjusting device for a guide device that is attached to the tip of an objective lens so that the position of the objective lens can be adjusted, and is brought into contact with an observation target so that the focal position of the objective lens coincides with the observation position. An objective lens fixing portion for fixing an objective lens to which a guide device having an adjustment screen having a predetermined pattern attached at the tip thereof is attached so that the position of the guide device can be adjusted; a visible light source that illuminates the adjustment screen; and the objective lens When the image sensor that captures the pattern that has passed and the tip surface of the guide device coincide with the position corresponding to the observation position, the pattern that passes through the objective lens is imaged on the image sensor. An adjusting device for a guide device including an image optical system is provided.
By forming a pattern on the image sensor, the guide device can be adjusted more easily with respect to the objective lens.

  According to the present invention, it is possible to easily arrange the distal end surface of the small-diameter distal end portion at an appropriate position with respect to the observation object in a state where the observation position is not fixed, and to easily and quickly There is an effect that an image can be acquired.

An adjustment screen of a guide device according to an embodiment of the present invention will be described below with reference to FIGS.
As shown in FIG. 1, the adjustment screen 1 according to the present embodiment is a position adjustment screen for a guide device 3 that is attached to the tip of an objective lens 2 so as to be capable of position adjustment in the optical axis direction.

  For example, as shown in FIG. 1, the objective lens 2 includes a large diameter portion 2a and a small diameter tip portion 2b having an outer diameter smaller than that of the large diameter portion 2a. As shown in FIG. 3, the objective lens 2 is used by being attached to the fluorescence microscope apparatus 4. In FIG. 1, reference numeral 2c denotes a mounting screw.

  For example, in the example shown in FIG. 2, the fluorescence microscope apparatus 4 is a laser scanning confocal microscope, and includes a light source unit 9 including a laser light source 5, a collimating optical system 6, a mirror 7, and a dichroic mirror 8, and the light source unit. A scanner 10 that two-dimensionally scans the laser light emitted from 9, a pupil projection lens 11 that condenses the laser light scanned by the scanner 10, and a laser beam condensed by the pupil projection lens 11 An imaging lens 12 for making the light substantially parallel; the objective lens 2 for condensing the laser light emitted from the imaging lens 12 on the specimen A; A dichroic mirror 13 for branching the fluorescence from the specimen A returning through the pupil projection lens 11 and the scanner 10 from the laser light, and the fluorescence branched by the dichroic mirror 13 And a light detector 14 for output.

  The light detection unit 14 includes a dichroic mirror 15 and a mirror 16 for separating the fluorescence for each wavelength, a barrier filter 17 for cutting the laser light included in the fluorescence, and a confocal lens for collecting the fluorescence transmitted through the barrier filter 17. 18, a confocal pinhole 19 disposed in an optically conjugate positional relationship with the focal position of the objective lens 2, and a photodetector 20 that detects fluorescence transmitted through the confocal pinhole 19. . In the figure, reference numeral 21 denotes an eyepiece optical system, and reference numeral 22 denotes a bright field illumination optical system.

As shown in FIG. 1, the guide device 3 includes a cylindrical portion 3 a that houses the small-diameter tip portion 2 b, a fitting portion 3 b that is fitted to the large-diameter portion 2 a of the objective lens 2, and the fitting portion. A push screw 23 that protrudes inward in the radial direction 3b and presses the outer surface of the large diameter portion 2a to fix the relative position between the objective lens 2 and the guide device 3 is provided.
A transparent window member 3c is provided at the distal end of the tubular portion 3a so as to close the distal end opening of the tubular portion 3a. By fixing the guide device 3 to the objective lens 2 and bringing the window member 3c into close contact with the specimen A, the focal position of the objective lens 2 can be quickly and accurately placed at a predetermined observation position on the specimen A, and the window By suppressing the pulsation of the specimen A brought into close contact with the member 3c, it is possible to acquire a clear image with little blur of the living specimen A.

The adjustment screen 1 includes a screen main body 1a that is brought into close contact with the front end surface of the window member 3c of the guide device 3, and an attachment / detachment portion 1b that removably fixes the screen main body 1a to the guide device 3.
The screen body 1a is, for example, a flat plate member made of a fluorescent material, and includes a flat surface 1c that is brought into close contact with the distal end surface of the window member 3c. As shown in FIG. 3, the flat surface 1c is provided with a recess 24 made of fine scratches. In the example shown in FIG. 3, the recesses 24 are provided in a lattice shape, but may have any shape such as a stripe shape or a dot shape.

  In the example shown in FIG. 1, the detachable portion 1 b is formed in a cylindrical shape into which the cylindrical portion 3 a of the guide device 3 is fitted. With the tubular portion 3a fitted to the detachable portion 1b and inserted to a position where the flat surface 1c abuts against the window member 3c at the tip of the tubular portion 3a, the flat surface 1c is in close contact with the window member 3c. The adjustment screen 1 can be kept attached to the guide device 3.

The adjustment method of the guide device 3 and the operation of the adjustment screen 1 according to the present embodiment configured as described above will be described below.
In order to adjust the position of the guide device 3 using the adjustment screen 1 according to the present embodiment, first, the attachment / detachment portion 1b of the adjustment screen 1 is fitted into the cylindrical portion 3a of the guide device 3, and the screen body 1a. The flat surface 1c is attached in a state where the flat surface 1c is in close contact with the front end surface of the window member 3c.

  Next, the small-diameter tip portion 2b of the objective lens 2 attached vertically downward to the fluorescence microscope apparatus 4 is accommodated in the cylindrical portion 3a of the guide device 3, and the fitting portion 3b of the guide device 3 is accommodated in the large diameter of the objective lens 2. Fit into the part 2a. Then, as shown in FIG. 4, the adjustment screen 1 attached to the tip of the guide device 3 is held in a state of being sandwiched between the stage 25. Thus, the objective lens 2 and the guide device 3 are moved relative to each other in the optical axis direction of the objective lens 2 by moving the objective lens 2 in the vertical direction using a focusing mechanism (not shown) of the fluorescence microscope apparatus 4. Can be moved.

  In this state, the fluorescence microscope apparatus 4 is operated, and the laser light emitted from the light source unit 9 is scanned two-dimensionally by the scanner 10. The laser light is emitted from the tip of the objective lens 2 and then focused on the focal position of the objective lens 2. Since the guide device 3 is disposed at the distal end of the objective lens 2, the laser light passes through the window member 3c of the guide device 3 and is in close contact with the window member 3c. Is incident on.

  Since the screen body 1a is made of a fluorescent material, fluorescence is generated when the laser light is irradiated. The generated fluorescence returns through the objective lens 2, the imaging lens 12, the pupil projection lens 11, and the scanner 10, and is detected by the photodetector 20. Thereby, a fluorescence image is acquired based on the swing angle position of the scanner 10 and the amount of fluorescence detected by the photodetector 20.

  In this case, in the fluorescence microscope apparatus 4, only the fluorescence that has passed through the focal position of the objective lens 2 is detected by the photodetector 20, so the focal position of the objective lens 2 is the tip surface of the window member 3 c of the guide device 3. If they do not match, only a blurred image of the pattern is obtained. Furthermore, when the deviation is large, the light intensity also decreases. Therefore, by operating the focusing mechanism of the fluorescence microscope apparatus 4 and moving the objective lens 2 and the guide apparatus 3 relative to each other in the optical axis direction, the focal position of the objective lens 2 becomes the position of the window member 3c of the guide apparatus 3. At the time of matching with the front end surface, the pattern of the recess 24 formed on the flat surface 1c of the screen body 1a is acquired as a fluorescent image. As a result of the fluorescence being reflected or diffusely reflected in the recess 24, a fluorescence image in which the pattern of the recess 24 is shaded is acquired.

  At this time, the operation of the focusing mechanism is stopped, the push screw 23 is fastened, and the fitting portion 3b of the guide device 3 is fixed to the large diameter portion 2a of the objective lens 2, whereby the cylindrical portion 3a of the guide device 3 is fixed. The guide device 3 and the objective lens 2 can be fixed in a state in which the focal position of the objective lens 2 is matched with the distal end surface of the distal window member 3c. In this state, by removing the adjustment screen 1 from the cylindrical portion 3a of the guide device 3, the adjustment of the guide device 3 is completed.

  After adjusting in this way, the focal position of the objective lens 2 is made to coincide with the outer surface of the sample A simply by bringing the tip surface of the window member 3c of the guide device 3 into close contact with the sample A actually observed. The fluorescence image of the outer surface of the specimen A can be acquired quickly and accurately. In addition, the pulsation of the specimen A can be suppressed by pressing the distal end surface of the window member 3c of the guide device 3 against the specimen A with a predetermined pressing force, and a fluorescent image with less blur can be acquired.

  In the present embodiment, the adjustment screen 1 is attached to the guide device 3 by fitting the attachment / detachment portion 1b provided on the adjustment screen 1 to the cylindrical portion 3a of the guide device 3. Instead, as shown in FIG. 5, the adjustment screen 1 may be simply constituted by a flat fluorescent plate and sandwiched between the guide device 3 and the stage 25.

Moreover, it is good also as bonding with the adhesive agent which peels easily instead of attaching by the fitting of the attachment / detachment part 1b, or in addition to this.
The adjustment screen 1 is made of a non-fluorescent material on the flat surface 1c of the screen body 1a made of a fluorescent material, in addition to the flat surface 1c of the screen body 1a made of a fluorescent material formed with a recess 24 made of a scratch. A pattern printed with ink, a pattern printed on a flat surface 1c of a screen body 1a made of a non-fluorescent material, and a non-fluorescent material printed on a flat surface 1c of a screen body 1a made of a fluorescent material. A transparent film (not shown) on which a pattern is formed by printing with ink made of a fluorescent material may be used.

  By printing and forming a pattern with an ink made of a fluorescent material, the amount of fluorescent light is highest when the focal position of the objective lens 2 coincides with the flat surface 1c of the screen body 1a. There is. In addition, by adopting a method of overlaying transparent films, the transparent films can be exchanged for each use, and problems such as cross contamination can be prevented.

  Further, the pattern is not limited to that shown in FIG. 3, and for example, as shown in FIG. 6, it is possible to adopt a pattern composed of the dot-shaped concave portions 24 that are fine in the center and rough in the periphery. Good. By doing in this way, even if a visual field range changes by changing the magnification, the ease of focusing can be improved.

  In the present embodiment, the screen body 1a of the adjustment screen 1 is sandwiched between the guide device 3 and the stage 25. Instead of this, as shown in FIG. A support base 26 that supports the fitting portion 3 b may be provided, and the adjustment screen 1 at the tip of the guide device 3 may be supported in a state of floating above the stage 25. By doing in this way, even if the small diameter front-end | tip part 2b of the objective lens 2 is lowered | lowered excessively by operation | movement of a focusing mechanism and contacts the window member 3c, an objective lens will only be damaged. It is possible to prevent an excessive force from acting on 2 and to prevent the objective lens 2 from being damaged.

  In this case, as shown in FIG. 8, a load sensor 27 may be disposed on the support base 26 and the pressing force transmitted from the objective lens 2 to the guide device 3 may be measured. By doing in this way, it can prevent that the small diameter front-end | tip part 2b of the objective lens 2 falls too much, and can avoid the failure | damage of the window member 3c.

  Further, in order to adjust the position of the adjustment screen 1 by pressing the adjustment screen 1 in a close contact state without attaching the adjustment screen 1 to the cylindrical portion 3a of the guide device 3, as shown in FIG. It is good also as providing the lift 28 moved up and down below the guide device 3 made. By doing so, the lift 28 is raised and lowered according to the difference in the length of the cylindrical portion 3 a depending on the type of the guide device 3, and the adjustment screen 1 placed on the lift 28 is attached to the window member 3 c of the guide device 3. It can be adjusted to any height position that will be in close contact. Reference numeral 29 is a set screw.

  Further, as shown in FIG. 10, a spring 30 that biases the lift 28 upward may be provided. By doing in this way, even if the length of the cylindrical portion 3a differs depending on the type of the guide device 3, the adjustment screen 1 is brought into close contact with the window member 3c of the guide device 3 by the urging force of the spring 30. Can be maintained. Reference numeral 31 denotes a set screw.

  Further, in the present embodiment, the guide device 3 is illustrated as having the fitting portion 3b fitted to the large-diameter portion 2a of the objective lens 2 and the push screw 23 for fixing both. Instead of this, as shown in FIG. 11, the present invention may be applied to a guide device 3 that is detachably fitted to the small-diameter tip 2 b of the objective lens 2.

  In the present embodiment, the guide device 3 and the objective lens 2 are fixed at a position focused on the pattern of the adjustment screen 1. Thereby, the focal position of the objective lens 2 can be accurately arranged on the distal end surface of the window member 3c of the guide device 3. However, when the observation position of the specimen A is arranged on the inner side rather than the outer surface of the specimen A, the focal position of the objective lens 2 is arranged on the distal end surface of the window member 3c of the guide device 3 Further, it is necessary to move the objective lens 2 and the guide device 3 relative to each other so that the focal position of the objective lens 2 is arranged in front of the tip surface of the window member 3c in the optical axis direction. In this case, the objective lens 2 and the guide device 3 may be moved relative to each other with high accuracy by the operation of a focusing mechanism or the like.

  Further, in the adjustment method of the guide device 3 according to the present embodiment, the method of attaching the guide device 3 to the objective lens 2 attached to the fluorescence microscope device 4 and adjusting using the fluorescence microscope device 4 has been described. . However, when the fluorescence microscope apparatus 4 is used, it is necessary to irradiate laser light that is excitation light, and from the viewpoint of preventing leakage of the laser light, it is necessary to perform sealing in a dark box or the like. For this reason, there is an inconvenience that adjustment work cannot be performed while visually confirming the objective lens 2 and the guide device 3.

  In order to solve this, for example, it is preferable to use an adjusting device 32 of the guide device 3 as shown in FIG. The adjusting device 32 includes an objective lens support 33 to which the objective lens 2 to which the guide device 3 with the adjusting screen 1 is attached, a visible light source 34, a diffusion plate 35 that diffuses visible light emitted from the visible light source 34, and A relay lens 36 that focuses visible light that has passed through the adjustment screen 1 and the objective lens 2 from the diffusion plate 35 to form an intermediate image, and a second lens that is disposed at the imaging position of the intermediate image and has a predetermined pattern. A screen 37 and an imaging lens 38 for condensing visible light transmitted through the second screen 37 and forming an image on the retina of the observer B are provided.

  By observing the imaging lens 38, the observer B moves the guide device 3 attached with the adjustment screen 1 in the optical axis direction with respect to the objective lens 2, thereby matching the pattern provided on the adjustment screen 1. A position where a sharp and clear observation image is acquired is searched. In this state, the position of the guide device 3 is further finely adjusted to simultaneously focus on the pattern of the adjustment screen 1 and the pattern of the second screen 37 to search for a position where a clear observation image is acquired. Thereby, the position of the front end surface of the window member 3c of the guide device 3 can be matched with the focal position of the objective lens 2 with high accuracy.

  In this case, since excitation light such as laser light is not used, it is not necessary to seal with a dark box or the like, and the observer B can perform adjustment work while directly viewing the objective lens 2 and the guide device 3. Therefore, the workability of the adjustment work can be improved.

  12 shows the upright adjustment device 32, but an inverted adjustment device 39 may be adopted as shown in FIG. Reference numeral 40 denotes a mirror, and reference numeral 41 denotes a relay lens. Further, as shown in FIG. 14, an adjusting device 43 in which an imaging element 42 such as a CCD is arranged instead of the imaging lens 38 may be employed. In this case, it is not necessary to consider the focusing function of the eyes of the observer B, and the second screen 37 is not necessary, so that the configuration can be simplified.

It is a typical longitudinal section showing the state where the adjustment screen concerning the 1st embodiment of the present invention was attached to the guide device. It is a whole block diagram which shows typically the fluorescence microscope apparatus containing the objective lens which attaches the guide apparatus of FIG. It is a front view which shows an example of the pattern formed in the flat surface of the screen for adjustment of FIG. It is a typical longitudinal cross-sectional view explaining the adjustment method of the guide apparatus using the adjustment screen of FIG. It is a typical longitudinal cross-sectional view which shows the case where it replaces with the adjustment screen of FIG. 1, and the adjustment screen which consists of fluorescent plates is used. It is a front view which shows the modification of the pattern formed in the flat surface of the screen for adjustment of FIG. It is a typical longitudinal cross-sectional view explaining the 1st modification of the adjustment method of the guide apparatus using the adjustment screen of FIG. It is a typical longitudinal cross-sectional view explaining the 2nd modification of the adjustment method of the guide apparatus using the adjustment screen of FIG. It is a typical longitudinal cross-sectional view explaining the 3rd modification of the adjustment method of the guide apparatus using the adjustment screen of FIG. It is a typical longitudinal cross-sectional view explaining the 4th modification of the adjustment method of the guide apparatus using the adjustment screen of FIG. It is a typical longitudinal cross-sectional view explaining the adjustment method at the time of using the guide apparatus different from the guide apparatus of FIG. It is a schematic diagram which shows the adjustment apparatus of the guide apparatus which concerns on the 1st Embodiment of this invention. It is a schematic diagram which shows the 1st modification of the adjustment apparatus of FIG. It is a schematic diagram which shows the 2nd modification of the adjustment apparatus of FIG.

Explanation of symbols

A specimen (observation target)
B Observer 1 Adjustment screen 1a Screen body 1b Detachable part 1c Flat surface 2 Objective lens 3 Guide device 24 Recess 32, 39, 43 Adjustment device 33 Objective lens support (objective lens fixing part)
34 Visible light source 36, 41 Relay lens (relay optical system)
37 Second screen 38 Imaging lens (imaging optical system)

Claims (12)

An adjustment method of a guide device that is attached to the tip of an objective lens so that the position of the objective lens can be adjusted, is brought into contact with an observation target, and matches the focal position of the objective lens with the observation position.
An adjustment screen having a predetermined pattern is fixed to a position where the optical axis of the objective lens is blocked when the guide device is attached to the distal end surface of the guide device,
Moving the guide device and the objective lens, to which the adjustment screen is fixed, in the optical axis direction,
The relative position between the objective lens and the guide device is fixed at a position where an image in focus with the pattern of the adjustment screen is obtained, or at a position further moved by a predetermined distance from the position. ,
A guide device adjustment method for removing the adjustment screen from the guide device.   The guide device adjustment method according to claim 1, wherein the guide screen and the objective lens are relatively moved while the adjustment screen is supported by the guide device.   The method for adjusting a guide device according to claim 1 or 2, wherein the guide device and the objective lens are relatively moved while measuring a load applied to either the guide device or the objective lens. An adjustment screen used in the adjustment method of the guide device according to any one of claims 1 to 3,
A screen body having a flat surface closely attached to the tip of the guide device;
An attaching / detaching portion for detachably attaching the screen body to the guide device;
An adjustment screen having a predetermined pattern on the flat surface. The screen body is made of a fluorescent material;
The adjustment screen according to claim 4, wherein the pattern is constituted by a recess provided on a flat surface. The screen body is made of a fluorescent material;
The adjustment screen according to claim 4, wherein the pattern is printed on a flat surface. The screen body is made of a fluorescent material;
The adjustment screen according to claim 4, wherein the pattern is printed on a transparent sheet covering a flat surface. The screen body is made of a non-fluorescent material;
The adjustment screen according to claim 4, wherein a pattern is printed on the flat surface with a fluorescent material.   The adjustment screen according to any one of claims 4 to 8, wherein the pattern is formed more finely in a central portion than in a peripheral portion. An adjustment device for a guide device, which is attached to the tip of the objective lens so that the position of the objective lens can be adjusted, is brought into contact with an observation target, and matches the focal position of the objective lens with the observation position,
An objective lens fixing unit that fixes an objective lens that is mounted so that the position of the guide device that has an adjustment screen having a predetermined pattern attached to the tip portion thereof can be adjusted; and
A visible light source that illuminates the adjustment screen;
Adjustment of a guide device including an imaging optical system that forms an image on the retina of an observer when the tip surface of the guide device coincides with a position corresponding to an observation position. apparatus. A relay optical system that forms an intermediate image of the pattern when a tip surface of the guide device coincides with a position corresponding to an observation position;
The adjusting device for a guide device according to claim 10, further comprising: a second screen having a second pattern arranged at a position of an intermediate image formed by the relay optical system. An adjustment device for a guide device, which is attached to the tip of the objective lens so that the position of the objective lens can be adjusted, is brought into contact with an observation target, and matches the focal position of the objective lens with the observation position,
An objective lens fixing unit that fixes an objective lens that is mounted so that the position of the guide device that has an adjustment screen having a predetermined pattern attached to the tip portion thereof can be adjusted; and
A visible light source that illuminates the adjustment screen;
An image sensor that photographs the pattern that has passed through the objective lens;
An adjusting device for a guide device, comprising: an imaging optical system that forms an image on the image sensor when the tip surface of the guide device coincides with a position corresponding to an observation position. .

Images