JP2008045877A - Method of manufacturing biosensor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method of manufacturing a biosensor for reducing its production cost and enhancing its productivity. <P>SOLUTION: A catalyst layer of this biosensor is transferred to a receptive layer in a catalyst-layer transfer process utilizing a transfer device 12 for a liquid body including a catalyst material. Further, a substrate electrode layer of the biosensor is applied with electroless-plating so as to cover the catalyst layer in a substrate-electrode-layer plating process utilizing a substrate plating bath 14 including a substrate electrode material. A coated electrode layer of the biosensor is applied with electroless-plating so as to cover the substrate electrode layer in a coated electrode layer plating process utilizing a coating/plating bath 16 including a coated electrode material. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a method for manufacturing a biosensor.

  Biosensors using enzymes, ion channels, and the like are widely used as sensors for detecting components in liquids. For example, a glucose sensor using enzyme activity is widely used as a sensor for detecting a glucose concentration in blood. A patient with diabetes, which is one of the adult diseases, has to measure blood glucose level several times a day and administer insulin and control diet based on the measurement result. Therefore, a glucose sensor that can easily detect the glucose concentration is indispensable for diabetic patients.

  The biosensor detects a change in a specific component as an electrical signal using the substrate specificity of the target liquid. The biosensor has a working electrode, a counter electrode, an enzyme, an electron transfer medium (mediator), and the like inside a storage chamber that stores a target liquid. The storage chamber introduces the target liquid into the interior using capillary force or the like. Enzymes and mediators are dissolved by the target liquid introduced into the storage chamber, and the enzyme reaction of the target liquid is started. The working electrode and the counter electrode detect and output a current associated with the enzyme reaction.

As the material of the electrode, a noble metal such as palladium, platinum, or gold is used for corrosion resistance. In Patent Document 1, a noble metal thin film is formed on the entire surface of a substrate by sputtering or vacuum vapor deposition, and laser processing is performed on the thin film to form a predetermined electrode pattern. In Patent Document 2, a heated relief printing plate is pressed against a noble metal foil deposited on a base film, and the foil is thermally transferred to a substrate to form a predetermined electrode pattern. In Patent Document 3, a predetermined electrode pattern is formed by sequentially depositing a nickel layer, a palladium nickel layer, and a platinum layer on a patterned copper foil on a substrate by a plating method.
JP 2005-114359 A Republished Patent Publication WO2004 / 017057 JP 2002-189012 A

  However, in Patent Document 1, it is necessary to form a noble metal thin film in a mask pattern region such as a metal mask in order to make the electrode film thickness and electrode film quality uniform. In Patent Document 2, in order to make the electrode shape uniform, it is necessary to form a noble metal thin film over substantially the entire surface of the base film. For this reason, there is a problem that the consumption of expensive noble metals is increased to increase the production cost of the biosensor.

  On the other hand, in Patent Document 3, although the consumption of noble metal can be limited to the electrode formation region, it requires a copper foil masking process, an exposure process, an etching process, etc., so the number of production processes is increased, and biosensor production is performed. There has been a problem of significantly lowering the properties.

  The present invention has been made to solve the above problems, and an object of the present invention is to provide a method of manufacturing a biosensor that enables reduction in production cost and improvement in productivity.

The biosensor manufacturing method of the present invention includes a catalyst layer forming step of wet-transferring a liquid material containing a catalyst material to an electrode forming region of a substrate to form a catalyst layer in the electrode forming region, and a plating solution containing the electrode material. And an electrode layer forming step of plating the electrode layer on the electrode forming region in contact with the catalyst layer.

  According to the method for manufacturing a biosensor of the present invention, the catalyst material and the electrode material are used for the respective electrode formation regions, so that the production cost of the biosensor can be reduced. In addition, since the electrode layer can be formed without requiring a masking step, an exposure step, an etching step, and the like, the number of manufacturing steps can be reduced, and the productivity of the biosensor can be improved.

  In this biosensor manufacturing method, the electrode layer forming step includes a coating layer forming step of plating a coating electrode layer on the electrode formation region by bringing a plating solution containing a coating electrode material made of a noble metal into contact with the catalyst layer. A configuration is preferred.

According to this biosensor manufacturing method, the amount of noble metal used can be reduced, and the production cost of the biosensor can be reduced.
In the biosensor manufacturing method, the electrode layer forming step includes a base layer forming step of plating a base electrode layer on the electrode forming region by bringing a plating solution containing a base electrode material made of a non-noble metal into contact with the catalyst layer. And a coating layer forming step of plating a coating electrode layer on the electrode formation region by bringing a plating solution containing a coating electrode material made of a noble metal into contact with the base electrode layer.

  According to this biosensor manufacturing method, since the base electrode layer is formed of the non-noble metal, the amount of the noble metal used for the electrode layer can be further reduced by the amount of the base electrode layer.

  In this biosensor manufacturing method, the catalyst layer may be a fine particle having catalytic activity or a layer of a coupling agent having catalytic activity for plating a coated electrode layer on the electrode forming region.

  In this biosensor manufacturing method, the catalyst layer forming step may be configured such that the catalyst layer is formed by wet-transferring a coupling agent having catalytic activity to the electrode forming region.

  According to this biosensor manufacturing method, the uniformity of the electrode layer and the adhesion between the electrode layer and the substrate can be improved by fixing the coupling agent having catalytic activity to the substrate. it can.

  In this biosensor manufacturing method, the catalyst layer forming step may be configured such that the catalyst layer is formed by wet-transferring a coupling agent for immobilizing the catalyst material to the electrode formation region.

According to this biosensor manufacturing method, the uniformity of the electrode layer and the adhesion between the electrode layer and the substrate can be improved by fixing the catalyst material to the substrate.
In this biosensor manufacturing method, in the catalyst layer forming step, a coupling agent for fixing the catalyst material to the substrate is formed on the entire surface, and the coupling agent is irradiated by irradiating ultraviolet rays outside the electrode formation region. It may be configured to be inactivated.

  According to this biosensor manufacturing method, the electrode forming region can be defined by irradiation with ultraviolet rays, and the size and shape of the electrode forming region can be formed with higher accuracy.

  In this biosensor manufacturing method, the catalyst layer forming step includes wet transfer of a liquid material containing fine particles having catalytic activity to an electrode forming region of a substrate and drying, and then irradiating the electrode forming region with ultraviolet rays to form fine particles. The structure which activates may be sufficient.

  According to this biosensor manufacturing method, a photocatalyst activated by ultraviolet rays can be used as a catalyst material. Therefore, the selection range of the catalyst material can be expanded, and the application range of the present manufacturing method can be expanded.

  Hereinafter, a biosensor 1 embodying the present invention will be described with reference to FIGS. FIG. 1 is an exploded perspective view of the biosensor 1, and FIG. 2 is a diagram illustrating the biosensor 1. In FIGS. 1 and 2, a thin gradation is given to the conductive region.

  In FIG. 1, the biosensor 1 is provided with a support plate 2 as a long substrate. On the upper side of the support plate 2, a strip-shaped spacer plate 3 formed in a size shorter than the support plate 2 and a cover plate 4 are sequentially stacked.

  The support plate 2, the spacer plate 3, and the cover plate 4 are each an insulating substrate. These three plates include, for example, polyethylene terephthalate, polyethylene naphthalate, polycarbonate, polyimide, polyethylene, polypropylene, polystyrene, polyvinyl chloride, polyoxymethylene, monomer cast nylon, polybutylene terephthalate, methacrylic resin, ABS resin, glass Etc. can be used.

  A notch 3a extending to the other end (base end) is formed at one end of the spacer plate 3 (lower left end in FIG. 1: tip). The notch 3a is surrounded by the support plate 2 and the cover plate 4 and forms a space (accommodating chamber S) having an opening (introduction port H) on the tip side. When the sample solution is introduced from the introduction port H, the storage chamber S stores the sample solution therein by a capillary force or the like.

  The cover plate 4 has a communication hole 4a that allows communication between the storage chamber S and the atmosphere. When the sample liquid is introduced from the introduction port H, the communication hole 4a allows the sample liquid to be smoothly accommodated by setting the internal pressure of the accommodation chamber S to atmospheric pressure.

  In FIG. 2, a receiving layer 2 a is formed on the upper surface of the support plate 2. The receiving layer 2a is a porous layer having pores, and absorbs the liquid component of the liquid droplets that have landed on the receiving layer 2a so that the fine particles in the liquid droplets remain on the upper surface of the receiving layer 2a. As the receiving layer 2a, for example, a material obtained by drying a mixture of at least one of alumina and alumina hydrate, silica particles, and a binder can be used.

  On the upper surface of the receiving layer 2a, three catalyst layers 5 extending over substantially the entire width in the longitudinal direction of the support plate 2 are formed. The catalyst layer 5 includes a working catalyst layer 5w, a counter catalyst layer 5c, and a detection catalyst layer 5r. Each catalyst layer 5 is a layer for performing electroless plating on the surface of the catalyst layer 5 (depositing an electrode material), and is a layer made of a catalyst material selected according to the electrode material.

Each of the working catalyst layer 5w, the counter catalyst layer 5c, and the detection catalyst layer 5r is formed in a band shape having a wide base end portion when viewed from the spacer plate 3 side.
The distal end portion of the working catalyst layer 5w is formed in a substantially L shape extending in the region of the storage chamber S when viewed from the spacer plate 3 side. The front end portion of the counter catalyst layer 5c is formed in a substantially F shape that sandwiches the front end of the working catalyst layer 5w when viewed from the spacer plate 3 side. The distal end portion of the detection catalyst layer 5r is formed in a strip shape extending in the region of the storage chamber S when viewed from the spacer plate 3 side.

In the present embodiment, the regions occupied by the working catalyst layer 5w, the counter catalyst layer 5c, and the detection catalyst layer 5r above the receiving layer 2a are defined as electrode formation regions.
As the catalyst material, for example, metal catalyst fine particles having a catalytic activity for the oxidizing action of a reducing agent in electroless plating and electrolessly depositing an electrode material can be used. As the metal catalyst, palladium, platinum, gold, nickel, copper, silver, or the like can be used.

  Moreover, a coupling agent for fixing the metal catalyst to the electrode layer forming region can be used as the catalyst material. The coupling agent has an amino organic functional group to form a metal catalyst and a metal complex, and holds the metal catalyst on the surface of the receiving layer 2a by chemical adsorption. As the coupling agent, γ-aminopropyltrimethoxysilane, N-β-aminoethyl-γ-aminopropyltrimethoxysilane, isopropyltri (N-aminoethyl-aminoethyl) titanate, trimethoxysilylpropyldiethylenetriamine, etc. are used. be able to.

  Further, the catalyst layer 5 may be one using silica fine particles or alumina fine particles as a metal catalyst carrier. For example, the above-mentioned coupling agent is adsorbed on the surface of silica fine particles, the silica fine particles adsorbed on the coupling agent are immersed in a solution composed of a compound of a metal catalyst, and the metal catalyst fixed on the surface of the fine particles is reduced. Can be used. The metal catalyst compound may be chloride, hydroxide or oxide of the above metal catalyst, and the reducing agent may be sodium hypophosphite, dimethylamine borane, sodium borohydride or the like. it can.

  Each catalyst layer 5 is formed by a wet transfer method. That is, each catalyst layer 5 is formed by bringing a stamp or the like impregnated with a liquid material containing the above catalyst material into contact with the electrode layer forming region, transferring the pattern of the liquid material containing the catalyst material, and drying it. . As a result, the use of the catalyst material can be limited only to the electrode layer forming region, and the use of useless catalyst material can be avoided.

A base electrode layer 6 that covers the entire corresponding catalyst layer 5 and a covering electrode layer 7 that covers the entire base electrode layer 6 are laminated on the upper side of each catalyst layer 5.
That is, the base electrode layer 6 and the covering electrode layer 7 corresponding to the working catalyst layer 5w are formed in the biosensor 1. The working electrode 7W is constituted by the working catalyst layer 5w and the corresponding base electrode layer 6 and covering electrode layer 7.

  In addition, the base electrode layer 6 and the covering electrode layer 7 corresponding to the counter catalyst layer 5c are formed in the biosensor 1. The counter electrode 7C is constituted by the counter catalyst layer 5c, the corresponding base electrode layer 6, and the covering electrode layer 7.

  In addition, the base electrode layer 6 and the covering electrode layer 7 corresponding to the detection catalyst layer 5r are formed in the biosensor 1. The detection electrode 7R is constituted by the detection catalyst layer 5r, the corresponding base electrode layer 6, and the covering electrode layer 7.

The coated electrode layer 7 protects each base electrode layer 6 from the sample solution when the sample solution is stored in the storage chamber S. When the storage chamber S is filled with the sample solution, the detection electrode 7R is connected to an external measurement device and detects whether or not the sample solution is filled in the storage chamber S. When the storage chamber S is filled with the sample solution, the working electrode 7W and the counter electrode 7C are connected to an external measuring device, respectively, and the working electrode 7W.
A predetermined voltage is applied between the electrode and the counter electrode 7C.

  The base electrode layer 6 is a layer made of a non-noble metal having conductivity (base electrode material: for example, nickel, copper, cobalt), and is formed by electroless plating using the catalyst layer 5 as a catalyst core. That is, each base electrode layer 6 is obtained by immersing the support plate 2 having each catalyst layer 5 in a plating bath containing the above base electrode material, and applying the base electrode material to the region of each catalyst layer 5, that is, the electrode formation region. It is formed by making it precipitate. As a result, the use of the base electrode material can be limited only to the electrode formation region, and the amount of the base electrode material used can be controlled by the shape and size of the catalyst layer 5.

  The covered electrode layer 7 is a layer made of a noble metal (for example, palladium, platinum, gold) having corrosion resistance, and is formed by electroless plating using the base electrode layer 6 as a catalyst core. That is, each coated electrode layer 7 is formed by immersing the support plate 2 having each base electrode layer 6 in a plating bath containing the above-described coated electrode material, and covering the region of each base electrode layer 6, that is, the electrode forming region. It is formed by precipitating the material. As a result, the use of the coated electrode material can be limited only to the electrode formation region, and the amount of the coated electrode material used can be controlled by the shape and size of the catalyst layer 5.

  A common reagent layer E is laminated on the surfaces of the working electrode 7W, the counter electrode 7C, and the detection electrode 7R, which are located inside the storage chamber S (regions with a dark gradation in FIG. 2). ). The reagent layer E contains oxidoreductase, mediator, and the like. The mediator functions as an electron transfer medium between the electrode and the enzyme. Examples of the mediator include one of redox organic or inorganic compounds such as alkali metal ferricyanide (potassium ferricyanide, lithium ferricyanide, sodium ferricyanide, etc.) p-benzoquinone, methylene blue, phenazine etsulfate, or the like. Two or more types can be used in combination. An oxidoreductase is an oxidoreductase of a target component (for example, glucose, lactic acid, uric acid, bilirubin, cholesterol, etc.), and uses glucose oxidase, lactate oxidase, cholesterol oxidase, bilirubin oxidase, glucose dehydrogenase, lactate dehydrogenase, etc. Can do.

  The reagent layer E is prepared by preparing an aqueous solution in which the components (for example, oxidoreductase and mediator) of the reagent layer E are dissolved, and dropping the aqueous solution onto the surfaces of the working electrode 7W, the counter electrode 7C, and the detection electrode 7R. It is formed by letting.

  When the sample liquid (region with gradation) is introduced from the introduction port H, the sample liquid is drawn into the storage chamber S by capillary force. The communication hole 4a makes the internal pressure of the storage chamber S an atmospheric pressure and avoids an increase in the internal pressure of the storage chamber S. That is, the inside of the storage chamber S is filled with the sample solution.

  When the storage chamber S is filled with the sample solution, the reagent layer E dissolves in the sample solution and generates a measurement solution as a measurement target. The measurement solution causes the enzyme reaction between the substrate of the sample solution and the oxidoreductase to reduce the mediator. When electrochemically oxidizing the reduced mediator, a current value corresponding to the substrate concentration of the sample solution is detected.

  Next, the manufacturing method of the biosensor 1 will be described with reference to FIGS. FIG. 3 is a diagram illustrating the biosensor 1 manufacturing system 10, and FIG. 4 is a flowchart illustrating the manufacturing process of the biosensor 1. 5-7 is explanatory drawing explaining each manufacturing process, respectively.

  In FIG. 3, the biosensor 1 manufacturing system 10 includes a supply roller R1 and a take-up roller R2. The supply roller R1 supplies the mother plate 2M that can cut out the plurality of support plates 2 to various processing apparatuses (rear stages), and the winding roller R2 sequentially winds up the mother plate 2M that has passed through various manufacturing processes.

  A cleaning device 11 for cleaning the receiving layer 2a is disposed following the supply roller R1. The cleaning device 11 performs a process for cleaning the surface of the mother plate 2M (receiving layer 2a) to obtain adhesion between the receiving layer 2a and the catalyst layer 5. The cleaning device 11 includes, for example, a light source that emits ultraviolet rays, and irradiates the receiving layer 2a with ultraviolet rays to decompose and clean (clean) organic molecules attached to the surface of the receiving layer 2a. Alternatively, the cleaning device 11 has a plasma source that generates oxygen plasma, exposes the receiving layer 2a to oxygen plasma, and decomposes and removes (cleans) organic molecules attached to the surface of the receiving layer 2a. Also good.

  A transfer device 12 is provided following the cleaning device 11. The transfer device 12 performs a wet transfer process for transferring the catalyst layer 5 to the receiving layer 2a (catalyst layer transfer step of FIG. 4: step S11). The transfer device 12 includes, for example, a stamp base 12 a and a stamp 12 b for reproducing the catalyst layer 5. As the stamp 12b, a stamp for micro contact printing can be used.

  As shown in FIG. 5, the transfer device 12 first immerses the stamp 12b in a liquid containing the catalyst material to adsorb the catalyst material to the stamp 12b, and the catalyst layer liquid made of the catalyst material is formed on the convex portion of the stamp 12b. A pattern 5L is formed (broken line in FIG. 5). Next, the stamp 12b is brought into contact with the receiving layer 2a, and the catalyst layer liquid pattern 5L formed on the convex portion is transferred to the receiving layer 2a.

  Thereby, the use of the catalyst material can be limited only to the electrode layer forming region, and the amount of the catalyst material used can be reduced. Moreover, since the masking process, the exposure process, the etching process and the like for patterning the catalyst layer 5 are not required, the number of production processes can be greatly reduced.

  In FIG. 3, a drying device 13 is provided following the transfer device 12. The drying device 13 performs drying / activation of the catalyst layer liquid pattern 5L according to the catalyst material (catalyst layer activation step of FIG. 4: step S12). The drying device 13 has a light source that irradiates, for example, an infrared region or ultraviolet rays. The drying device 13 irradiates the catalyst layer liquid pattern 5L (for example, a liquid film of a metal catalyst complexed with a coupling agent or a coupling agent) with light in the infrared region, and the solvent or dispersion medium of the catalyst layer liquid pattern 5L. Is evaporated to form the catalyst layer 5 (coupling agent or metal catalyst is fixed to the receiving layer 2a). Alternatively, the drying device 13 irradiates the catalyst layer liquid pattern 5L (liquid film in which metal fine particles are dispersed) with ultraviolet rays, decomposes and removes organic molecules that protect the metal fine particles, or activates an optically active metal catalyst. Thus, the catalyst layer 5 is formed. Thereby, the catalyst layer 5 made of the activated catalyst material can be formed only in the electrode layer forming region.

In the present embodiment, the catalyst layer forming step is configured by the catalyst layer activation step and the catalyst layer transfer step.
In FIG. 3, a base plating bath 14 is provided at the subsequent stage of the drying device 13. The base plating bath 14 has an electroless plating solution 14L containing a metal ion of a non-noble metal (base electrode material: for example, nickel, copper, cobalt), and performs electroless plating using the catalyst layer 5 as a catalyst core ( Base electrode layer plating step: Step S13).

The electroless plating solution 14L includes non-noble metal salts, reducing agents such as sodium hypophosphite, hydrazine, sodium borohydride, carboxylic acids such as sodium acetate, sodium citrate, sodium succinate, sodium potassium tartrate, and water Acid salts, amines such as ethylenediamine, phenylenediamine, and EDTA, and other base electrode material complexing agents.

  As shown in FIG. 6, the base plating bath 14 immerses the mother plate 2M (catalyst layer 5) for a predetermined time to deposit the base electrode material in the region of the catalyst layer 5, that is, the electrode formation region. As a result, the use of the base electrode material can be limited only to the electrode formation region, and the amount of the base electrode material used can be controlled by the shape and size of the catalyst layer 5.

  In FIG. 3, a drying device 15 is provided following the base plating bath 14. The drying device 15 dries the mother plate 2M immersed in the base plating bath 14 (base electrode layer drying step: step S14). The drying device 13 has a light source that emits light in the infrared region, for example, irradiates the mother plate 2M with light in the infrared region, evaporates the solvent contained in the deposited base electrode material, and forms the base electrode layer 6. Form.

In the present embodiment, the base layer forming step is constituted by the base electrode layer drying step and the base electrode layer plating step.
In FIG. 3, a coating plating bath 16 is provided following the drying device 15. The coating plating bath 16 has an electroless plating solution 16L containing a metal ion of a noble metal (coated electrode material: for example, palladium, platinum, gold), and performs electroless plating using the base electrode layer 6 as a catalyst nucleus ( Clothing electrode layer plating step: Step S15). Electroless plating solution 16L includes noble metal salts, reducing agents such as sodium hypophosphite, hydrazine, sodium borohydride, carboxylic acids such as sodium acetate, sodium citrate, sodium succinate, sodium potassium tartrate, and their water solubility. Salts, amines such as ethylenediamine, phenylenediamine, EDTA, and other complexing agents for coated electrode materials.

  As shown in FIG. 7, in the coating plating bath 16, the mother plate 2 </ b> M (the base electrode layer 5) is immersed for a predetermined time to deposit the cover electrode material in the region of the base electrode layer 6, that is, the electrode formation region. Since the base electrode material (nickel, copper, cobalt) has higher catalytic activity than the coated electrode material (palladium, platinum, gold), it is not necessary to further activate the surface of the base electrode layer 6 and electroless The coated electrode material can be deposited by a plating process. Thus, the use of the coated electrode material can be limited only to the electrode formation region, and the amount of the coated electrode material used can be controlled by the shape and size of the base electrode layer 6 (catalyst layer 5).

  In FIG. 3, a drying device 17 and a die cutting device 18 are provided following the coating plating bath 16. The drying device 17 dries the mother plate 2M immersed in the coating plating bath 16 (coated electrode layer drying step: step S16). The drying device 17 has a light source that irradiates light in the infrared region, for example, irradiates the mother plate 2M with light in the infrared region, evaporates the solvent contained in the deposited coated electrode material, and forms the coated electrode layer 7. Form. The die cutting device 18 includes, for example, a die cutting base 18a and a mold 18b for cutting out the outer shape of the support plate 2, and performs the outer cutting of the support plate 2 from the mother plate 2M conveyed to the die cutting base 18a. .

  In this embodiment, the covering electrode layer drying step and the covering electrode layer plating step constitute a covering layer forming step, and the covering layer forming step and the base layer forming step constitute an electrode layer forming step. ing.

Next, effects of the present embodiment configured as described above will be described below.
(1) According to the above embodiment, the catalyst layer 5 of the biosensor 1 is transferred to the receiving layer 2a by the catalyst layer transfer process using the wet transfer of the liquid material containing the catalyst material. The base electrode layer 6 of the biosensor 1 is electrolessly plated so as to cover the catalyst layer 5 by a base electrode layer plating process using a base plating bath 14 containing a base electrode material. The coated electrode layer 7 of the biosensor 1 is electrolessly plated so as to cover the base electrode layer 6 by a coated electrode layer plating process using a coated plating bath 16 containing a coated electrode material.

  Therefore, the catalyst material, the base electrode material, and the coated electrode material are used for the respective electrode formation regions. Therefore, the usage amount of the catalyst material, the base electrode material, and the coated electrode material can be kept to the minimum amount, and the production cost of the biosensor 1 can be reduced. Moreover, the catalyst layer 5, the base electrode layer 6, and the covering electrode layer 7 can be formed without requiring a masking step, an exposure step, an etching step, and the like. Therefore, the number of production steps of the biosensor 1 can be reduced, and the productivity of the biosensor 1 can be improved.

  (2) Moreover, according to the said embodiment, a catalyst layer transfer process transfers the catalyst layer liquid pattern 5L over the whole electrode formation area. In the base electrode layer plating step, the base electrode layer 6 is formed so as to cover the entire catalyst layer 5 over the entire electrode formation region. In the covering electrode layer plating step, the covering electrode layer 7 is formed so as to cover the entire base electrode layer 6 over the entire electrode formation region. Therefore, corrosion of the base electrode layer 6 due to the measurement solution can be prevented by the coated electrode layer 7 made of a noble metal. Therefore, the base electrode material can be composed of a low-cost non-noble metal that does not have corrosion resistance. Therefore, the production cost of the biosensor 1 can be further reduced.

  (3) Moreover, according to the above embodiment, the catalyst layer liquid pattern 5L is wet-transferred to the porous receiving layer 2a. Therefore, the catalyst layer liquid pattern 5L of the stamp 12b can be more reliably transferred to the mother plate 2M, and the reproducibility of the size and shape of the catalyst layer 5 can be obtained.

  (4) According to the above embodiment, in the catalyst layer transfer step, the coupling agent is transferred as a part of the catalyst layer 5, and the metal catalyst is fixed to the receiving layer 2a. Therefore, the catalyst material can be fixed in the electrode formation region, and the film thickness uniformity of the base electrode layer 6 and the adhesion between the base electrode layer 6 and the receiving layer 2a can be improved.

  (5) According to the embodiment, in the catalyst layer activation step, the catalyst layer liquid pattern 5L is irradiated with ultraviolet rays. Therefore, the photocatalyst activated by light in the ultraviolet region can be used as the catalyst material, and the selection range of the catalyst material can be expanded. Therefore, the application range of this manufacturing method can be expanded.

In addition, you may change the said embodiment as follows.
In the above embodiment, the catalyst layer liquid pattern 5L is transferred over the entire electrode formation region. For example, the catalyst layer liquid pattern 5L is transferred to substantially the whole of the mother plate 2M including the electrode formation region, and the region other than the electrode formation region is irradiated with ultraviolet rays so that the catalyst layer in the region other than the electrode formation region. The liquid pattern 5L may be deactivated. According to this, the catalyst layer 5 can be prescribed | regulated by irradiation of an ultraviolet-ray, and the size and shape of an electrode formation area can be formed with a higher precision.

  In the above embodiment, the biosensor 1 has a working electrode, a counter electrode, and a detection electrode. For example, a reference electrode may be provided instead of the detection electrode, or a configuration in which a reference electrode is added separately may be used. Or the structure which has only a working electrode and a counter electrode may be sufficient.

In the above embodiment, the support plate 2 has the receiving layer 2a. For example, the upper surface of the support plate 2 may be subjected to a surface treatment such as an ultraviolet irradiation treatment or a plasma irradiation treatment to give a desired wettability to the catalyst layer liquid pattern 5L. That is, when the transferred catalyst layer liquid pattern 5L forms a pattern having a desired size, the support plate 2 may not have the receiving layer 2a.

  In the above embodiment, the base electrode layer 6 has a single layer structure. However, the present invention is not limited to this, and the base electrode layer 6 may be formed in a multilayer structure, and a barrier layer that suppresses diffusion of the base electrode material may be interposed between the base electrode layer and the covering electrode layer.

The exploded perspective view explaining the biosensor of this embodiment. Similarly, the figure explaining a biosensor. The figure explaining the manufacturing system of a biosensor similarly. Similarly, the flowchart explaining the manufacturing process of a biosensor. Similarly, the figure explaining the manufacturing process of a biosensor. Similarly, the figure explaining the manufacturing process of a biosensor. Similarly, the figure explaining the manufacturing process of a biosensor.

Explanation of symbols

L ... Sample solution, S ... Storage chamber, 1 ... Biosensor, 2 ... Support plate as substrate, 5 ... Catalyst layer, 6 ... Base electrode layer, 7 ... Coated electrode layer.

Claims (8)

A catalyst layer forming step of wet-transferring a liquid material containing a catalyst material to an electrode forming region of a substrate to form a catalyst layer in the electrode forming region;
An electrode layer forming step in which a plating solution containing an electrode material is brought into contact with the catalyst layer to plate the electrode layer on the electrode forming region;
A method for producing a biosensor, comprising: In the manufacturing method of the biosensor of Claim 1,
The electrode layer forming step includes
A biosensor manufacturing method comprising a coating layer forming step of plating a coating solution containing a coated electrode material made of a noble metal in contact with the catalyst layer and plating the coated electrode layer on the electrode forming region. In the manufacturing method of the biosensor of Claim 1,
The electrode layer forming step includes
A base layer forming step of plating a base electrode layer on the electrode forming region by bringing a plating solution containing a base electrode material made of a non-noble metal into contact with the catalyst layer;
A coating layer forming step of plating the coating electrode layer on the electrode formation region by bringing a plating solution containing a coating electrode material made of a noble metal into contact with the base electrode layer;
A method for producing a biosensor, comprising: In the manufacturing method of the biosensor of Claims 1-3,
The catalyst layer is
The electrode forming region is a layer of catalytically active fine particles or a catalytically active coupling agent for plating a coated electrode layer.
A biosensor manufacturing method characterized by the above. In the manufacturing method of the biosensor of Claim 4,
The catalyst layer forming step includes
A catalyst having a catalytic activity is wet-transferred to the electrode forming region to form the catalyst layer;
A biosensor manufacturing method characterized by the above. In the manufacturing method of the biosensor of Claim 4,
The catalyst layer forming step includes
A coupling agent for immobilizing the catalyst material is wet transferred to the electrode forming region to form the catalyst layer;
A biosensor manufacturing method characterized by the above. In the manufacturing method of the biosensor of Claim 4,
The catalyst layer forming step includes
A coupling agent for fixing the catalyst material to the substrate is formed on the entire surface, and the coupling agent is inactivated by irradiating ultraviolet rays outside the electrode formation region.
A biosensor manufacturing method characterized by the above. In the biosensor manufacturing method according to claim 4,
The catalyst layer forming step includes
A liquid containing fine particles having catalytic activity is wet-transferred to the electrode formation region of the substrate and dried, and the electrode formation region is irradiated with ultraviolet rays to activate the fine particles.
A biosensor manufacturing method characterized by the above.

Images