JP2008024647A - Adiponectin secretion promoting agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a composition for eating/feeding capable of actively increasing the adiponectin concentration in blood. <P>SOLUTION: The adiponectin secretion promoting agent is an oil-and-fat composition containing an SUU-type triglyceride (S is a 20-24C saturated fatty acid residue, and U is a ≥16C unsaturated fatty acid residue) as an active component in an amount of 5-30 wt.% in the base oil-and-fat. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an adiponectin secretion promoter comprising SUU type triglyceride as an active ingredient.

  Japan's dietary habits have changed greatly in recent years, and the intake of animal foods such as meat has increased, while the intake of plant foods such as rice, vegetables, and beans accompanying the withdrawal from Japanese food has decreased. Against this background, patients with lifestyle-related diseases such as diabetes, arteriosclerosis and obesity are increasing.

  By the way, it is proposed that the adipose tissue of a living body is not a mere temporary storage of energy but a “secretory tissue” that secretes various physiologically active substances collectively called adipocytokines (Non-patent Document 1). Adipocytokines include TNF-α, which is involved in insulin resistance, leptin, angiotensinogen, which is associated with hypertension, and PAI-1, which is an important regulator of the fibrinolytic system and promotes thrombus formation. Among them, “adiponectin” has attracted the most attention in recent years. Adiponectin is a secreted protein specific to adipose tissue and has a collagen-like structure.

  One of the important roles of adiponectin physiological action has been shown to improve insulin resistance by regulating the secretion of PAI-1, TNF-α, resistin and free fatty acids (Non-patent Document 2). , 3). In a test using mice, it has been reported that adiponectin acts directly on the area of the atherosclerotic lesion mainly composed of macrophages and suppresses foaming of macrophages and atherosclerosis (Non-patent Document 4). From these, it is assumed that each disease can be prevented by positively increasing the adiponectin concentration.

  On the other hand, behenic acid, which is a long-chain saturated fatty acid, is known to have a low digestive absorption rate in the digestive tract of animals due to its high melting point. A low-absorbency triglyceride containing behenic acid as a constituent fatty acid using this property has been disclosed (Patent Document 1). These triglycerides can be used as a low-calorie food when used as a substitute for normal fats and oils, but the recognition of the effect is limited to the amount of behenic acid ingested without being absorbed. In addition, there is no disclosure of an example of using SUU type triglycerides in which the binding position of fatty acid to glycerin is specified at a low concentration. There is no suggestion of adiponectin secretion.

Annals of the New York Academy of Sciences 892, 146-154, 1999. Matsuzawa, Soy Protein Research, 6, 1-10, 2003. Shimomura, History of Medicine, 207, 647-652, 2003. Takahashi, History of Medicine, 192,541-545, 2001. JP-A-1-85040

  In view of this situation, if a substance capable of more strongly promoting the secretion of adiponectin can be elucidated, the increase in the blood concentration of adiponectin makes it possible to treat diabetes, arteriosclerotic diseases, develop preventive drugs and foods. That is, an object of the present invention is to find a substance capable of promoting the secretion of adiponectin and to develop an oral product useful for the treatment and prevention of various diseases based on this adiponectin secretion promoting substance.

  In order to solve the above-mentioned problems, the present inventors have found for the first time that oral administration of a small amount of SUU type triglyceride strongly enhances blood adiponectin concentration. In accordance with this finding, it was found that SUU-type triglycerides can be used as an active ingredient of an adiponectin secretion promoter, and the present invention was completed.

That is, the present invention is (1) an adiponectin secretion promoter comprising SUU type triglyceride as an active ingredient. In the formula, S is a saturated fatty acid residue having 20 to 24 carbon atoms, and U is an unsaturated fatty acid residue having 16 or more carbon atoms.
(2) The secretion promoter of (1), wherein S is a behenic acid residue.
(3) The secretion promoter of (1), wherein U is an oleic acid residue.
(4) The secretion promoter according to (1), which is an oil or fat composition containing 5 to 30% by weight of SUU type triglyceride.
(5) A food containing the secretion promoter of (1), with a health label based on the adiponectin secretion promoting effect.
It is.

  Since the secretion enhancer provided by the present invention has a high secretion promoting effect of adiponectin, it can provide functional agents and foods and drinks using it, and can increase blood adiponectin concentration by oral intake of these. .

  Hereinafter, the present invention will be described in detail. The adiponectin secretion promoter of the present invention is characterized by containing SUU-type triglyceride as an active ingredient. The fatty acid residue indicated as S in the present invention is a saturated fatty acid residue having 20 to 24 carbon atoms, but this effect is weak for saturated fatty acids having 18 or less carbon atoms, and for saturated fatty acids having 26 or more carbon atoms, the melting point In addition, the versatility is reduced, and there are few sources of fatty acids, so it is not practical. In view of the effect and feasibility, behenic acid having 22 carbon atoms is preferred.

  The fatty acid residue indicated as U is an unsaturated fatty acid residue having 16 or more carbon atoms, but unsaturated fatty acids having 14 or less carbon atoms are not highly practical because they have few sources. The upper limit of the carbon number of U is not particularly set, but considering the supply source, it is preferably 22 or less and most preferably 18 or less. The degree of unsaturation is not particularly limited, but considering the oxidative stability of fatty acids, extremely polyunsaturated fatty acids are difficult to use, preferably unsaturated below dienoic acid, more preferably monounsaturated acids, Oleic acid is most preferred.

  The active ingredient of the present invention is represented by the SUU type in which the fatty acid residue represented by S is ester-bonded to the hydroxyl group at the 1- or 3-position of glycerol, and the fatty acid residue represented by U is ester-bonded to the remaining two hydroxyl groups of glycerol. 1 long chain saturated-2 long chain unsaturated triglyceride. In addition, in the notation of this invention specification, the 1st position and the 3rd position are not distinguished about the coupling | bonding position to the glycerol of a fatty acid.

  The present invention is characterized by containing a small amount of SUU-type triglyceride in fats and oils. And by this ingestion, adiponectin secretion can be promoted and the blood adiponectin concentration can be increased without greatly affecting the digestibility. The effective intake as an adiponectin secretion enhancer varies depending on the purpose of use, the target of use, and the form, but by increasing the adiponectin concentration in the blood by containing 5 to 30% by weight, preferably 10 to 20% by weight in the base oil or fat Can do.

  When SUU-type triglycerides are present in oils and fats in excess of 30% by weight, other effects due to low digestion and absorption such as a decrease in calorie intake are exhibited, and problems such as absorption inhibition of fat-soluble vitamins occur. Furthermore, the melting point of the oil and fat composition containing SUU-type triglyceride is increased, and the versatility is lacking. Further, if it is 5% by weight or less in fats and oils, the effect relating to the increase in blood adiponectin concentration is not clearly shown, so that it is inappropriate.

  The present invention is characterized by containing a small amount of SUU type triglyceride in fats and oils, but the base fats and oils are edible animal and vegetable fats and oils such as soybean oil, sunflower oil, rapeseed oil, canola oil, rice bran oil, safflower oil, palm Oil, palm kernel oil, coconut oil, fish oil, milk fat, cocoa butter, etc. are not limited. In many drugs, ingestion in excess of the proper amount may cause safety problems, whereas the active ingredient of the present invention is a triglyceride using natural plant-derived ingredients. From the viewpoint of safety, the upper limit of intake is hardly a problem.

  The SUU type triglyceride which is an active ingredient of the present invention is a fatty acid or ester of a highly hardened oil of Hyelin rapeseed oil or fish oil, or further purified by distillation, using a general unsaturated fatty acid as a source of S fatty acid. By using a lipase having a specificity at positions 1 and 3 as a fatty acid source such as rapeseed oil, canola oil, soybean oil, sunflower oil, safflower oil, hyolein sunflower oil, hyolein safflower oil, etc. Obtained by transesterification. The obtained active ingredient can be concentrated by solvent fractionation, which is preferable.

  The secretagogue of the present invention can be used for various uses of foods, drugs or feeds. The form at that time varies depending on the melting point of the base oil and fat and the addition amount of the SUU type triglyceride of the present invention, but the form in which the liquid oil is used as it is, the form of a liquid such as emulsion or suspension, cream, margarine, shortening, hard It can take the form of common food ingredients such as butter. The food using this secretagogue is preferably a food with a relatively high fat content, for example, mayonnaise, dressings, various creams, chocolate and the like. In addition, it can mix | blend with confectionery, such as baked confectionery, bread, etc., and can also be used as a capsule.

  The standard for intake of the secretion promoter of the present invention and foods containing the same is not particularly set, and an adiponectin secretion promoting effect can be obtained by using it instead of normal foods and oil and fat compositions in foods. . Examples of the intake of the secretagogue to be targeted are 5 to 100 g, preferably 10 to 50 g per day.

  Examples of the present invention will be described below, but the technical scope of the present invention is not limited by these examples. In the following, “%” means “% by weight” unless otherwise specified.

[Production Example 1]
A highly hardened oil of high erucin rapeseed oil containing 45% of unsaturated fatty acids having 22 carbon atoms was hydrolyzed and esterified to obtain fatty acid ethyl ester. This fatty acid ester was rectified to obtain a fraction containing 97.9% of a saturated fatty acid ester having 20 to 24 carbon atoms in the acyl group. 70 parts of this fatty acid ester fraction was mixed with 30 parts of high oleic sunflower oil, transesterified using a 1,3-position specific enzyme agent, and then the fatty acid ester was distilled off to give an iodine value of 45. A reaction oil was obtained. Further fractionation was performed, and the low melting point fraction was collected at a yield of 42.4% and purified by a conventional method (test fat and oil). The test fats and oils have a melting point of 24 ° C. and an iodine value of 55.8. The fatty acid composition and triglyceride (TG) composition are shown in Table 1.

(Table 1) Fatty acid and triglyceride composition of test fats and oils

[Test Example 1]
The effect of the test fats and oils on blood adiponectin concentration was investigated by animal experiments. Twelve 7-week-old SD male rats (sold by Japan SLC Co., Ltd.) were used as model animals. After one week of preliminary breeding, the group was divided into a comparative oil and fat group (6 animals) and a test oil and fat group (6 animals) so that the average body weights between groups were almost equal. The dietary feed was 28% fat based on the AIN-93G composition. These were ad libitum for 6 weeks. The diet composition is shown in Table 2 below.

(Table 2) Test meal composition

  Six weeks later, blood was collected from the abdominal aorta. Plasma was prepared according to a conventional method, and the amount of adiponectin in the blood was measured with an ELISA method kit (manufactured by Otsuka Pharmaceutical Co., Ltd.). As shown in Table 3, an increase in blood adiponectin concentration was observed by replacement of SUU type triglyceride. On the other hand, the fecal production rate of rats was only slightly increased from 8.0% (comparison test group) to 9.4% (test group), and it was estimated that this effect was not caused by low absorption of behenic acid. From the above results, it was shown that SUU type triglyceride has an adiponectin secretion promoting action.

(Table 3) Blood adiponectin concentration and fecal production rate

[Example 1] Production of mayonnaise While mixing 30 parts of egg yolk with 25 parts of vinegar, 4 parts of salt and 1 part of sodium glutamate, and separately stirring 28 parts of test oil prepared in Production Example 1, 112 parts of rapeseed oil The mayonnaise which has an adiponectin secretion promoting effect can be prepared by gradually adding the mixed oil mixed with.

[Example 2] Production of cream In 51 parts of water, 4 parts of skim milk powder, 9 parts of test oil prepared in Production Example 1, 36 parts of milk fat, 0.2 part of sugar ester, 0.2 part of lecithin, sodium hexametaphosphate 0 .1 part of the mixed solution is heated to 70 ° C., stirred with a homomixer (manufactured by Tokushu Kika Kogyo Co., Ltd.) at 10,000 rpm for 1 minute, and pre-emulsified. Next, this solution is homogenized using a high-pressure homogenizer under a force of 10 kgf / cm 2 and then sterilized by heating at 144 ° C. for 4 seconds. This solution is rapidly cooled to 5 ° C. and then aged at 5 ° C. overnight to prepare a cream having an adiponectin secretion promoting effect.

Claims (5)

Adiponectin secretion promoter containing SUU-type triglyceride as an active ingredient. In the formula, S is a saturated fatty acid residue having 20 to 24 carbon atoms, and U is an unsaturated fatty acid residue having 16 or more carbon atoms. The secretagogue according to claim 1, wherein S is a behenic acid residue. The secretagogue according to claim 1, wherein U is an oleic acid residue. The secretion promoter according to claim 1, which is an oil or fat composition containing 5 to 30% by weight of SUU type triglyceride. A food comprising the secretion enhancer according to claim 1 with a health label based on an adiponectin secretion promoting effect.