JP2008009186A - Container holder - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a container holder capable of easily and speedily adjusting the position of a specimen container in three axial directions of the direction of an optical axis and directions orthogonal to the optical axis with respect to the objective lens of a micro-imaging apparatus. <P>SOLUTION: The container holder 1 is installed or mounted on the micro-imaging apparatus and used for imaging or observing a specimen held in at least one of the specimen holding parts of the specimen container. The container holder 1 includes: a container accommodating member 2 that accommodates the specimen container 5; and a positioning member 4 having a positioned part 4c, which is positioned to the objective lens of the micro-imaging apparatus, and used to position the specimen container to the container storage member by freely removably fitting it in the container accommodating member. The specimen container is positioned in the direction of the optical axis of the objective lens by the positioning member, and also the specimen container is positioned in the directions orthogonal to the optical axis of the objective lens by the positioning member. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a container holder that is installed or attached to a microscopic imaging device and used for imaging or observing a sample held in a sample container.

  Conventionally, in the field of biochemistry or pathology, for example, when a biological sample is imaged or observed with a microscopic imaging device, a sample container holding the biological sample is placed on a stage or attached to a base portion mounted on the stage to perform imaging or the like (For example, refer to Patent Document 1).

JP 2003-107364 A

  By the way, the sample container used for imaging and observation of such a biological sample is placed on the stage of the microscopic imaging apparatus so as to be optically coupled with the microscopic imaging apparatus to clearly image or observe the sample in the sample container. Each time, it is necessary to adjust the position of the objective lens in the plane direction along the stage surface and in the optical axis direction orthogonal to the stage surface. However, this position adjustment has a problem that it requires skill and takes time to adjust, and it cannot be easily performed by anyone. In addition, when observing and imaging a plurality of samples over a long period of time, the microscopic imaging device may have to be installed on the stage while sequentially exchanging the plurality of sample containers. There has been a problem that the position adjustment work becomes complicated.

  The present invention has been made in view of the above, and easily and quickly adjusts the position of the sample container in the three-axis directions, ie, the optical axis direction and the direction perpendicular to the optical axis, with respect to the objective lens of the microscopic imaging apparatus. It is an object of the present invention to provide a container holder that can be used.

  In order to solve the above-described problems and achieve the object, a container holder according to claim 1 is installed or attached to a microscopic imaging device, and images or observes a sample held in at least one sample holder of the sample container. A container holder for holding the sample container and a positioning portion positioned with respect to the objective lens of the microscopic imaging apparatus, and is detachably assembled to the container holder A positioning member for positioning the sample container with respect to the container housing member, and positioning the sample container in the optical axis direction of the objective lens by the positioning member, and the positioning unit to position the sample container by the positioning unit. Positioning is performed in a direction perpendicular to the optical axis of the objective lens.

  In the container holder according to claim 2, in the above invention, the container housing member includes a bottom wall and a side wall having an internal thread formed on an inner periphery, and the positioning member includes the internal thread. A male screw part that is screwed to the outer periphery, the positioning part is a light shielding hole that engages the tip of the objective lens and shields light from the outside and guides the light emitted from the sample to the objective lens It is characterized by that.

  In the container holder according to claim 3, in the above invention, the light shielding hole has an internal thread formed on an inner periphery, and the microscopic imaging device is formed on an outer periphery of a lens holder of the objective lens. It has the external thread part screwed together in the internal thread part of a hole, It is characterized by the above-mentioned.

  In order to solve the above-described problems and achieve the object, a container holder according to claim 4 is installed or attached to a microscopic imaging apparatus, and images a sample held in at least one sample holding part of the sample container. Or it is a container holder used for observation, Comprising: The microscopic imaging which positions the sample container with respect to the container storage member by detachably assembling the container storage member for storing the sample container and the container storage member An objective lens of the apparatus, and the sample container is positioned by the objective lens in an optical axis direction and a direction orthogonal to the optical axis.

  According to a fifth aspect of the present invention, in the container holder according to the fifth aspect, the container housing member has a bottom wall and a side wall formed with an internal thread portion on the inner surface, and the objective lens is disposed on the internal thread portion. A male screw part to be screwed is formed.

  According to a sixth aspect of the present invention, in the container holder according to the sixth aspect, the container housing member is provided with a displacement prevention means for preventing a displacement between the bottom wall and the microscopic imaging device. Features.

  The container holder according to claim 7 is characterized in that, in the above invention, the positioning member is provided with imaging means for imaging the light emitted from the sample at a predetermined position in the light shielding hole. To do.

  The container holder according to claim 8 is characterized in that, in the above invention, the imaging means is a convex lens or a plurality of optical fiber bundles having a microlens at one end.

  The container holder according to claim 9 is characterized in that, in the above invention, the image forming means has a changing mechanism for changing at least one of a focal length, an enlargement magnification, and a viewing angle. .

  The container holder according to claim 10 is characterized in that, in the above invention, the container holder further comprises display means for displaying light emitted from the sample.

  The container holder according to an eleventh aspect of the invention is characterized in that in the above invention, a heating means is further provided.

  A container holder according to a twelfth aspect of the present invention is characterized in that, in the above invention, the container holder further includes a fluid circulating means for circulating the fluid necessary for culturing the sample to the sample container.

  The container holder according to the present invention positions the sample container in the optical axis direction with respect to the objective lens of the microscopic imaging device by the positioning member, and orthogonally intersects the optical axis with respect to the objective lens by the positioning unit. Positioning in the direction makes it possible to easily and quickly adjust the position of the sample container in the three axis directions, ie, the optical axis direction and the direction orthogonal to the optical axis with respect to the objective lens.

(Embodiment 1)
Hereinafter, Embodiment 1 concerning the container holder of this invention is demonstrated in detail, referring FIGS. 1-11. FIG. 1 is a perspective view illustrating a container holder according to the first embodiment. FIG. 2 is a cross-sectional view of a container housing member used in the container holder according to the first embodiment. FIG. 3 is a perspective view of a positioning member used in the container holder according to the first embodiment. FIG. 4 is an exploded perspective view showing an assembled state of the container holder according to the first embodiment.

  As shown in FIG. 1, the container holder 1 includes a container housing member 2 and a positioning member 4, and is used when a sample held in the sample container 5 is imaged or observed with a microscopic imaging device.

  The container accommodating member 2 accommodates the sample container 5 in a replaceable manner, and has a bottom wall 2a and a side wall 2b as shown in FIGS. 2 and 4, and is formed by the bottom wall 2a and the side wall 2b. The sample container 5 is accommodated in the space. The side wall 2b has an opening 2c in the upper part, and a female screw part 2d is formed up to the center of the inner surface in the height direction.

  The positioning member 4 is detachably assembled to the container housing member 2 to position the sample container 5 with respect to the container housing member 2. As shown in FIGS. 1 and 3, the positioning member 4 has a male threaded portion 4b that is screwed into the female threaded portion 2d on the outer periphery of a cylindrical main body 4a, and shields light from outside the container holder 1 A light blocking hole 4c is formed in the center for guiding light from bioluminescence or chemiluminescence emitted from the sample held in the container 5 to the microscopic imaging device. The light blocking hole 4c serves as a positioning portion for positioning with respect to the objective lens 8b of the microscopic imaging device 8, and the inner surface is painted black.

  Here, the sample container 5 is a container (petri dish) made of a transparent material in which the inner dish 5a is covered with the outer dish 5b, and a biological sample such as a cell is held inside.

  As shown in FIG. 4, the container holder 1 configured in this way accommodates the sample container 5 in the container housing member 2 through the opening 2c, and then covers the positioning member 4 to screw the male screw portion 4b onto the female screw portion 2d. The positioning member 4 is assembled. As a result, the container holder 1 sandwiches the sample container 5 between the bottom wall 2 a and the positioning member 4, and positions the sample container 5 at a fixed position with respect to the bottom wall 2 a of the container housing member 2.

  The container holder 1 that positions the sample container 5 in this way is installed at a preset position on the stage 8a of the microscopic imaging device 8, as shown in FIG. At this time, the stage 8a and the objective lens 8b are previously separated from each other by a working distance or more. Next, the revolver 8c is operated to move the objective lens 8b having a desired magnification directly above the light shielding hole 4c of the container holder 1 as shown in FIG. Then, the fine focus handle is operated while viewing the image with the eyepiece 8d to focus the light emitted from the sample, and then image is taken.

  Here, in the microscopic imaging device 8, by focusing, the tapered portion 8e at the tip of the objective lens 8b comes into contact with the upper edge of the light shielding hole 4c as shown in FIG. For this reason, in the container holder 1, when the objective lens 8b is lowered as the microscopic imaging device 8 is focused, the positioning member 4 is orthogonal to the optical axis of the objective lens 8b by the tapered portion 8e engaged with the light shielding hole 4c. Moved in the direction. For this reason, the light blocking hole 4c serves as a positioning portion that positions the positioning member 4, and thus the container holder 1, in a direction perpendicular to the optical axis. The microscopic imaging device 8 focuses in advance with the low-magnification objective lens 8b, and then focuses with the high-magnification objective lens 8b.

  At this time, if the sample container 5 having the same size is used, the container holder 1 can be positioned at the same position in the optical axis direction of the microscopic imaging device 8 simply by being placed on the stage 8a. Accordingly, in the container holder 1, the sample container 5 is positioned by the positioning member 4 in the three axial directions, ie, the optical axis direction of the objective lens 8b and the direction orthogonal to the optical axis, so that the sample container 5 is positioned on the optical axis of the objective lens 8b. The position can be adjusted easily and quickly in three directions perpendicular to the direction and the optical axis, and a clear image of light emitted from the sample can be observed and imaged.

  Here, as shown in FIG. 8, the container holder 1 is preferably provided with an elastic ring 3 on the lower outer periphery of the bottom wall 2 a of the container housing member 2. As shown in FIG. 9, the elastic ring 3 is a misalignment prevention means made of an elastic body having a C-shaped cross section, and the C-shaped portion serves as a suction portion 3a. For this reason, when the container holder 1 is installed on the stage 8a, the adsorbing portion 3a is adsorbed on the upper surface of the stage 8a, and the positional deviation in the direction perpendicular to the optical axis of the microscopic imaging device 8 can be prevented. Here, when the stage 8a is made of a magnetic material, the container holder 1 can be microscopically provided by providing a magnetic rubber or a ring-shaped permanent magnet below the bottom wall 2a of the container housing member 2 instead of the elastic ring 3. A positional shift in the direction orthogonal to the optical axis of the imaging device 8 can be prevented.

  On the other hand, as shown in FIG. 10, the positioning member 4 may form a light-shielding female screw portion 4d in the light-shielding hole 4c. In this way, if a male screw portion that is screwed into the light-shielding female screw portion 4d is formed in advance on the objective lens 8b, the container holder 1 is positioned on the objective lens 8b of the microscopic imaging device 8 as shown in FIG. The member 4 can be detachably attached by screwing. Therefore, in the container holder 1, the sample container 5 is positioned by the positioning member 4 in the three axial directions, ie, the optical axis direction of the objective lens 8b and the direction orthogonal to the optical axis. Position adjustment can be performed easily and quickly.

(Embodiment 2)
Next, Embodiment 2 according to the container holder of the present invention will be described in detail with reference to FIGS. Although the container holder 1 of the first embodiment uses the positioning member 4, the container holder 10 of the second embodiment uses the objective lens 8b of the microscopic imaging device 8 as the positioning member. FIG. 12 is a perspective view of the container holder according to the second embodiment. FIG. 13 is an exploded perspective view illustrating an assembled state of the container holder according to the second embodiment. Here, in the embodiment described below, the same components as those in the first embodiment are denoted by the same reference numerals.

  As shown in FIGS. 12 and 13, the container holder 10 includes a container housing member 2 and an objective lens 8 b serving as a positioning member. The objective lens 8b has a male screw portion 8f attached to the revolver 8c of the microscopic imaging device 8 at one end and a male screw portion 8g to be screwed into the female screw portion 2d of the container housing member 2 at the other end. .

  Therefore, as shown in FIG. 14, the container holder 10 can be mounted in the three axial directions of the optical axis direction and the direction orthogonal to the optical axis only by attaching the objective lens 8b to the revolver 8c of the microscopic imaging device 8 by the male screw portion 8f. Positioned. Therefore, the container holder 10 can easily and quickly adjust the optical position of the sample container 5 with respect to the microscopic imaging device 8. In this case, the container holder 10 performs focusing when observing or imaging the sample in the sample container 5 by rotating the container housing member 2 in the left-right direction and adjusting the screwing amount with the male screw portion 8g.

(Embodiment 3)
Next, Embodiment 3 according to the container holder of the present invention will be described in detail with reference to FIGS. In the container holder 1 of the first embodiment, the positioning member 4 has the light shielding hole 4c. However, in the container holder 15 of the third embodiment, a condensing lens is provided in the light shielding hole 4c. FIG. 15 is a cross-sectional view illustrating the container holder according to the third embodiment. FIG. 16 is a perspective view of a modified example of the container holder according to the third embodiment. 17 is a perspective view showing an optical fiber lens used in the container holder of FIG.

  As shown in FIG. 15, the container holder 15 is provided with a condensing lens 6 using a convex lens in the light shielding hole 4c. The condensing lens 6 forms an image on the object-side focal point of the microscopic imaging device 8 with light emitted by bioluminescence or chemiluminescence emitted from the sample held in the sample container 5. Therefore, the container holder 15 can easily and quickly adjust the optical position of the sample container 5 with respect to the microscopic imaging device 8 when performing the focus adjustment of the microscopic imaging device 8. At this time, the container holder 15 positions the sample container 5 in the three-axis directions of the objective lens 8b and the direction orthogonal to the optical axis by the positioning member 4 in the same manner as the container holder 1 of the first embodiment. To do.

  Here, as shown in FIG. 16, the container holder 15 may use an optical fiber lens 7 instead of the condenser lens 6. As shown in FIG. 17, the optical fiber lens 7 is a bundle of a plurality of optical fibers 7 a each having a micro lens 7 b formed at one end, and the other end of each optical fiber 7 a serves as an imaging plane F. For this reason, the optical fiber lens 7 uses a microlens 7b whose focal plane is located at the optical fiber lens 7 so that the triaxial positioning described in the first embodiment is possible. The optical fiber lens 7 is moved along the light shielding hole 4c along with the focusing by the driving mechanism, and is immersed in the light shielding hole 4c, as shown in FIG. 18, and at least one of a focal length, an enlargement magnification, or a viewing angle. To change. For this reason, when the optical fiber lens 7 is used, the container holder 15 can perform the optical position adjustment of the sample container 5 with respect to the microscopic imaging device 8 more easily and more quickly, and the sample container 5 A sharp image of light emitted from the held sample can be observed and imaged more by the microscopic imaging device 8. Here, the container holder 15 shown in FIG. 16 is drawn in a state in which the optical fiber lens 7 protrudes from the light shielding hole 4c, but the imaging plane F is submerged in the light shielding hole 4c in a state of focusing. The same applies to the drawings described below.

(Embodiment 4)
Next, a fourth embodiment of the container holder of the present invention will be described in detail with reference to FIGS. Although the container holder 1 of the first embodiment includes the container housing member 2 and the positioning member 4, the container holder 20 of the fourth embodiment further emits light emitted from the sample held in the sample container 5. A display device that receives light and displays the received light is provided. FIG. 19 is a perspective view of the container holder according to the fourth embodiment. FIG. 20 is a perspective view showing how the display device is operated while holding the container holder of FIG. FIG. 21 is a perspective view showing a use state of the container holder shown in FIG.

  In the container holder 20, the display device 21 is attached to the positioning member 4 having the container housing member 2 and the optical fiber lens 7 shown in FIG. The display device 21 is positioned on the main body 4 a of the positioning member 4 and placed on the upper side wall 2 b of the container housing member 2. In the display device 21, the light receiving means provided on the main body 21 a abuts on the imaging surface F of the optical fiber lens 7, and the light emitted from the sample held in the sample container 5 is emitted by the bioluminescence or chemiluminescence by the light receiving means. 7 is received and the light emission image is displayed on the screen 21b. The display device 21 has an operation button for performing an operation of displaying a light emission image on the screen 21b, and an alarm lamp for notifying an abnormality in the amount of light emitted from the sample. At this time, in the optical fiber lens 7, the other end of each optical fiber 7 a is an image plane F. Therefore, the display device 21 can display the light received by the light receiving means on the screen 21b as it is.

  For this reason, as shown in FIG. 20, the container holder 20 is operated by the operator to operate the operation button of the display device 21, and the light emitted from the sample held in the sample container 5 is displayed on the screen 21b. Can be made.

  On the other hand, the container holder 20 removes the display device 21 after being placed on the stage 8a as shown in FIG. 21 when observing or imaging light emitted from the sample held in the sample container 5 by the microscopic imaging device 8. Then, the fine focus handle is operated to finely move the objective lens 8b to focus the light emitted from the sample. In the container holder 20, the optical fiber lens 7 forms an image of the light emitted from the sample held in the sample container 5 at the object side focal point of the microscopic imaging device 8, and the positioning member 4 causes the sample container 5 to be optical axis of the objective lens 8 b. Since the positioning is performed in the three axis directions orthogonal to the direction and the optical axis, the optical position adjustment of the sample container 5 with respect to the microscopic imaging device 8 can be performed easily and quickly.

(Embodiment 5)
Next, a fifth embodiment of the container holder of the present invention will be described in detail with reference to FIG. Although the container holder 1 of Embodiment 1 was provided with the container accommodating member 2 and the positioning member 4, the container holder 25 of Embodiment 5 was further provided with a heat retaining means. FIG. 22 is a perspective view of the container holder according to the fifth embodiment.

  As shown in FIG. 22, the container holder 25 is provided with heating devices 26 and 27 above and below the container housing member 2 and the positioning member 4 shown in FIG. The heating device 26 is attached on the positioning member 4, has a window 26 b made of a transparent member at the top of the main body 26 a, and a heater for heating the window 26 b is provided inside. The heating device 27 is attached to the lower surface of the container housing member 2 and holds the sample held in the stored sample container 5 at a predetermined culture temperature. Here, both the heating devices 26 and 27 have a control means and a switch for setting the heating temperature to a predetermined temperature.

  Therefore, the container holder 25 can culture the sample in a state where the sample held in the sample container 5 is held at a predetermined temperature. When the light emitted from the sample is observed or imaged by the microscopic imaging device 8, the container holder 25 is removed from the heating devices 26 and 27 and placed on the stage 8a. Then, the container holder 25 positions the sample container 5 in the three-axis directions of the objective lens 8b and the direction perpendicular to the optical axis by the positioning member 4 in the same manner as the container holder 1 of the first embodiment. Therefore, the optical position of the sample container 5 with respect to the microscopic imaging device 8 can be easily and quickly adjusted, and a clear image of light emitted from the sample can be observed and captured.

  Here, when culturing the sample, when the container holder 25 heats the sample to a predetermined culture temperature by the heating device 27, water vapor generated by evaporation of the liquid in the sample container 5 is condensed on the window 26b. For this reason, the container holder 25 may be prevented from being observed in a state where the heating devices 26 and 27 are mounted. Accordingly, in such a case, the container holder 25 turns on the switch of the heating device 26 and heats the window 26b with the heater to prevent condensation.

(Embodiment 6)
Next, a sixth embodiment of the container holder of the present invention will be described in detail with reference to FIGS. Although the container holder 25 of the fifth embodiment includes the container housing member 2 and the positioning member 4, the container holder 30 of the sixth embodiment further includes a fluid circulation device. FIG. 23 is a perspective view of the container holder according to the sixth embodiment. FIG. 24 is a perspective view of a sample container used in the container holder according to the sixth embodiment.

  As shown in FIG. 23, the container holder 30 is provided with a fluid circulation device 31 in the container housing member 2 and the positioning member 4 shown in FIG. The fluid circulation device 31 circulates a fluid necessary for culturing a sample to the sample container 35, and supplies a chemical solution to the sample container 35 between the main body 31 a provided with a control means and the container housing member 2. 31b, a gas supply tube 31c for supplying a gas such as carbon dioxide to the sample container 35 and a recovery tube 31d for recovering the supplied chemical solution or gas are connected.

  As shown in FIG. 24, the sample container 35 is a container made of a transparent material in which an inner dish 35a is covered with an outer dish 35f, and a biological sample such as a cell is held therein. The inner dish 35a is formed with a chemical liquid injection hole 35b, a gas injection hole 35c, and a recovery hole 35d for recovering the injected chemical liquid or gas on the side wall.

  Accordingly, the container holder 30 supplies the chemical solution or gas to the container housing member 2 by the chemical solution supply tube 31b or the gas supply tube 31c in a state where the container holder 30 is installed on the stage 8a of the microscopic imaging device 8. As a result, the chemical solution or gas supplied to the container housing member 2 passes through the chemical solution injection hole 35b or the gas injection hole 35c of the sample container 35 accommodated in the space formed by the bottom wall 2a and the side wall 2b. After the chemical solution or gas is supplied to the biological sample, it is discharged from the recovery hole 35d together with the waste material discharged from the biological sample, and is recovered to the main body 31a of the fluid circulation device 31 by the recovery tube 31d. In this way, the container holder 30 circulates the chemical solution or gas between the fluid circulation device 31 and the sample container 35 to culture the sample held in the sample container 35.

  When the microscopic imaging device 8 observes or images the light emitted from the biological sample being cultured, the container holder 30 operates the fine focus handle of the microscopic imaging device 8 to finely move the objective lens 8b to emit the biological sample. You only need to focus the light. At this time, in the container holder 30, as in the container holder 1 of the first embodiment, the positioning member 4 positions the sample container 5 in the three-axis directions of the objective lens 8b and the direction orthogonal to the optical axis. To do. For this reason, the container holder 30 can easily and quickly adjust the optical position of the sample container 5 with respect to the microscopic imaging device 8, and observes and captures a clear image of light emitted from the biological sample. be able to.

It is a perspective view which shows the container holder concerning Embodiment 1. FIG. It is sectional drawing of the container accommodating member used with the container holder concerning Embodiment 1. FIG. It is a perspective view of the positioning member used with the container holder concerning Embodiment 1. FIG. It is a disassembled perspective view which shows the assembly | attachment state of the container holder concerning Embodiment 1. FIG. It is a perspective view which shows the use condition which installed the container holder shown in FIG. 1 in the microscopic imaging device. It is a perspective view which shows the state which adjusted the position of the objective lens to the light-shielding hole of a container holder. It is sectional drawing which shows the state which the objective lens contact | abutted to the upper edge of the light shielding hole by focusing of a microscopic imaging device. It is a perspective view which shows the 1st modification of the container holder which provided the position shift prevention means which consists of an elastic body in the container accommodating member. It is principal part sectional drawing which cut | disconnected the position shift prevention means of FIG. 8 on the diameter. It is a perspective view which shows the 2nd modification of the container holder which formed the internal thread part in the light shielding hole of the positioning member. It is a perspective view which shows the use condition which attached the container holder shown in FIG. 10 to the micro imaging device. It is a perspective view which shows the container holder concerning Embodiment 2. FIG. It is a disassembled perspective view which shows the assembly | attachment state of the container holder concerning Embodiment 2. FIG. It is a perspective view which shows the use condition which attached the container holder shown in FIG. 12 to the microscopic imaging device. It is sectional drawing which shows the container holder concerning Embodiment 3. It is a perspective view which shows the modification of the container holder concerning Embodiment 3. FIG. It is a perspective view which shows the optical fiber lens used with the container holder of FIG. It is a perspective view which shows the use condition of the container holder shown in FIG. It is a perspective view which shows the container holder concerning Embodiment 4. FIG. 20 is a perspective view showing how the display device is operated while holding the container holder of FIG. It is a perspective view which shows the use condition of the container holder shown in FIG. It is a perspective view which shows the container holder concerning Embodiment 5. FIG. It is a perspective view which shows the container holder concerning Embodiment 6. FIG. It is a perspective view which shows the sample container used with the container holder concerning Embodiment 6. FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Container holder 2 Container accommodation member 2a Bottom wall 2b Side wall 2c Opening 2d Female screw 3 Elastic ring 3a Adsorption part 4 Positioning member 4a Main body 4b Male screw 4c Light-shielding hole 4d Female screw part 5 Sample container 5a Inner plate 5b Outer plate 6 Condensing lens 7 Optical fiber lens 7a Optical fiber 7b Microlens lens 8 Microscopic imaging device 8a Stage 8b Objective lens 8c Revolver 8d Eyepiece 8e Tapered part 8f, 8g Male thread part 10, 15 Container holder 20 Container holder 21 Display device 21a Main body 21b Screen 25 Container holder 26, 27 Heating device 30 Container holder 31 Fluid circulation device 31a Main body 31b Chemical liquid supply tube 31c Gas supply tube 31d Recovery tube 35 Sample container 35a Inner dish 35b Chemical liquid injection hole 35c Gas injection hole 35d Recovery hole 35f Outer dish The image plane

Claims (12)

A container holder used for imaging or observation of a sample installed or attached to a microscopic imaging device and held in at least one sample holder of a sample container,
A container housing member for housing the sample container;
A positioning member that is positioned with respect to the objective lens of the microscopic imaging apparatus, and that is detachably assembled to the container housing member and positions the sample container with respect to the container housing member;
With
A container holder characterized by positioning the sample container in the optical axis direction of the objective lens by the positioning member, and positioning the sample container in a direction orthogonal to the optical axis of the objective lens by the positioning unit. The container housing member has a bottom wall and a side wall in which an internal thread is formed on the inner periphery,
The positioning member has a male threaded portion that is screwed into the female threaded portion on the outer periphery. The container holder according to claim 1, wherein the container holder is a light shielding hole that leads to an objective lens. The light shielding hole is formed with a female screw portion on the inner periphery,
The container holder according to claim 2, wherein the microscopic imaging device has a male screw portion that is formed on an outer periphery of a lens holder of the objective lens and is screwed into a female screw portion of the light shielding hole. A container holder used for imaging or observation of a sample installed or attached to a microscopic imaging device and held in at least one sample holder of a sample container,
A container housing member for housing the sample container;
An objective lens of the microscopic imaging device that detachably attaches to the container housing member and positions the sample container with respect to the container housing member;
A container holder characterized by positioning the sample container in an optical axis direction and a direction orthogonal to the optical axis by the objective lens. The container housing member has a bottom wall and a side wall in which an internal thread portion is formed on the inner surface,
The container holder according to claim 4, wherein the objective lens is formed with a male screw portion that is screwed into the female screw portion.   The container holder according to claim 2, wherein the container housing member is provided with a displacement prevention means for preventing a positional displacement between the bottom wall and the microscopic imaging apparatus.   The container holder according to claim 2, wherein the positioning member is provided with an image forming unit that forms an image of light emitted from the sample at a predetermined position in the light shielding hole.   The container holder according to claim 7, wherein the imaging means is a convex lens or a plurality of optical fiber bundles each having a microlens at one end.   The container holder according to claim 8, wherein the imaging unit includes a changing mechanism that changes at least one of a focal length, an enlargement magnification, and a viewing angle.   The container holder according to claim 7, further comprising display means for displaying light emitted by the sample.   Furthermore, a heating means is provided, The container holder as described in any one of Claims 1-10 characterized by the above-mentioned.   The container holder according to any one of claims 1 to 10, further comprising a fluid circulation means for circulating a fluid necessary for culturing the sample to the sample container.

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