JP2008003051A5 - - Google Patents

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JP2008003051A5
JP2008003051A5 JP2006175494A JP2006175494A JP2008003051A5 JP 2008003051 A5 JP2008003051 A5 JP 2008003051A5 JP 2006175494 A JP2006175494 A JP 2006175494A JP 2006175494 A JP2006175494 A JP 2006175494A JP 2008003051 A5 JP2008003051 A5 JP 2008003051A5
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fluorescence
observation
microscope
intensity
photometry
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Abandoned
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JP2006175494A
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JP2008003051A (en
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Priority to JP2006175494A priority Critical patent/JP2008003051A/en
Priority claimed from JP2006175494A external-priority patent/JP2008003051A/en
Priority to US11/588,646 priority patent/US7696491B2/en
Publication of JP2008003051A publication Critical patent/JP2008003051A/en
Publication of JP2008003051A5 publication Critical patent/JP2008003051A5/ja
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Claims (16)

低蛍光なイマージョン物質を用いてなる蛍光観察又は蛍光測光システムであって、
前記低蛍光なイマージョン物質が次の条件式(1-1)を満足し、且つ、
前記低蛍光なイマージョン物質におけるｄ線（５８７．５６ｎｍ）における屈折率ｎｄが１．７０以上であることを特徴とする蛍光観察又は蛍光計測システム。
ＢIM'／ＢIM≦０．７ …(1-1)
ただし、ＢIM'は前記低蛍光なイマージョン物質の自家蛍光の強度の平均値、ＢIMは従来一般的に使用されているイマージョン物質の自家蛍光の強度の平均値である。 A fluorescence observation or fluorescence photometry system using a low-fluorescence immersion material,
The low-fluorescence immersion material satisfies the following conditional expression (1-1), and
A fluorescence observation or fluorescence measurement system, wherein a refractive index nd at d-line (587.56 nm) in the low-fluorescence immersion material is 1.70 or more.
B IM '/ B IM ≦ 0.7 (1-1)
However, B IM 'is an average intensity value of auto-fluorescence from the low immersion substance, B IM is an average value of the intensity of the autofluorescence of immersion substance generally used conventionally. 前記低蛍光なイマージョン物質が、ジヨードメタンに硫黄を溶解させてなることを特徴とする請求項１に記載の蛍光観察又は蛍光計測システム。The fluorescence observation or fluorescence measurement system according to claim 1, wherein the low-fluorescence immersion material is obtained by dissolving sulfur in diiodomethane. 蛍光観察又は蛍光測光方法において、次の工程Ａ，Ｂ，Ｃからなることを特徴とする蛍光観察又は蛍光測光方法。In the fluorescence observation or fluorescence photometry method, the fluorescence observation or fluorescence photometry method characterized by comprising the following steps A, B, and C.
Ａ．生細胞を用いた蛍光を発する試料を選択する工程。  A. A step of selecting a sample that emits fluorescence using living cells.
Ｂ．前記工程Ａで選択した試料を観察又は測光するためのアプリケーション、及び次の条件式(1-1)を満足し、且つ、ｄ線（５８７．５６ｎｍ）における屈折率ｎｄが１．７０以上である低蛍光なイマージョン物質を用いてなる蛍光観察又は蛍光測光システムを選択する工程。  B. An application for observing or measuring the sample selected in the step A and the following conditional expression (1-1) are satisfied, and the refractive index nd at the d-line (587.56 nm) is 1.70 or more. A step of selecting a fluorescence observation or fluorescence photometry system using a low fluorescence immersion material.
Ｃ．前記工程Ｂで選択したアプリケーション及びシステムを用いて、前記工程Ａで選択した試料を蛍光観察又は蛍光測光する工程。  C. A step of fluorescence observation or fluorescence photometry of the sample selected in the step A using the application and system selected in the step B.
Ｂ      B IMIM '／Ｂ'/ B IMIM ≦０．７ …(1-1)≦ 0.7… (1-1)
ただし、Ｂ  However, B IMIM 'は前記低蛍光なイマージョン物質の自家蛍光の強度の平均値、Ｂ'Is the average value of the autofluorescence intensity of the low-fluorescence immersion material, B IMIM は従来一般的に使用されているイマージョン物質の自家蛍光の強度の平均値である。Is an average value of the intensity of autofluorescence of an immersion material generally used conventionally. 前記工程Ａで選択する生細胞を用いた蛍光を発する試料が、次の条件式(2-1)，(3-1)の少なくとも一方を満足することを特徴とする請求項３に記載の蛍光観察又は蛍光測光方法。The fluorescence according to claim 3, wherein the sample that emits fluorescence using the living cells selected in step A satisfies at least one of the following conditional expressions (2-1) and (3-1). Observation or fluorescence photometry.
（Ｓ−ｓ）／（Ｂ＋ｂ）≦５ …(2-1)      (S−s) / (B + b) ≦ 5 (2-1)
３Ｂ      3B IMIM ／Ｂ≧０．２ …(3-1)/B≧0.2 (3-1)
ただし、Ｓは前記試料が発する蛍光の強度の平均値、ｓは該蛍光の強度の揺らぎ幅、Ｂは試料が存在しない背景ノイズの強度の平均値、ｂは該背景ノイズの強度の揺らぎ幅、Ｂ  However, S is the average value of the intensity of the fluorescence emitted from the sample, s is the fluctuation width of the intensity of the fluorescence, B is the average value of the intensity of the background noise in the absence of the sample, b is the fluctuation width of the intensity of the background noise, B IMIM は従来一般的に使用されているイマージョン物質の自家蛍光の強度の平均値である。Is an average value of the intensity of autofluorescence of an immersion material generally used conventionally. 前記工程Ｂで選択したアプリケーションがＦＲＥＴ（Fluorescence Resonance Energy Transfer：蛍光共鳴エネルギー移動）であることを特徴とする請求項４に記載の蛍光観察又は蛍光測光方法。The fluorescence observation or fluorescence photometry method according to claim 4, wherein the application selected in the step B is FRET (Fluorescence Resonance Energy Transfer). 前記工程Ｂで選択したシステムが蛍光顕微鏡システムであることを特徴とする請求項５に記載の蛍光観察又は蛍光測光方法。6. The fluorescence observation or fluorescence photometry method according to claim 5, wherein the system selected in step B is a fluorescence microscope system. 前記工程Ｂで選択したシステムが全反射顕微鏡システムであることを特徴とする請求項５に記載の蛍光観察又は蛍光測光方法。6. The fluorescence observation or fluorescence photometry method according to claim 5, wherein the system selected in step B is a total reflection microscope system. 前記工程Ｂで選択したシステムが蛍光顕微鏡と全反射顕微鏡のいずれか一方を２つ又は両方を１つずつ有し、前記試料を挟んで対物光学系が対向配置された顕微鏡システムとして構成されていることを特徴とする請求項５に記載の蛍光観察又は蛍光測光方法。The system selected in the step B is configured as a microscope system having either one or both of a fluorescence microscope and a total reflection microscope and an objective optical system facing each other with the sample interposed therebetween. The fluorescence observation or fluorescence photometric method according to claim 5. 前記工程Ｂで選択したアプリケーションがカルシウムイメージングであることを特徴とする請求項４に記載の蛍光観察又は蛍光測光方法。The fluorescence observation or fluorescence photometric method according to claim 4, wherein the application selected in step B is calcium imaging. 前記工程Ｂで選択したシステムが蛍光顕微鏡システムであることを特徴とする請求項９に記載の蛍光観察又は蛍光測光方法。The fluorescence observation or fluorescence photometric method according to claim 9, wherein the system selected in the step B is a fluorescence microscope system. 前記工程Ｂで選択したシステムが全反射顕微鏡システムであることを特徴とする請求項９に記載の蛍光観察又は蛍光測光方法。The fluorescence observation or fluorescence photometric method according to claim 9, wherein the system selected in step B is a total reflection microscope system. 前記工程Ｂで選択したシステムが蛍光顕微鏡と全反射顕微鏡のいずれか一方を２つ又は両方を１つずつ有し、前記試料を挟んで対物光学系が対向配置された顕微鏡システムとして構成されていることを特徴とする請求項９に記載の蛍光観察又は蛍光測光方法。The system selected in the step B is configured as a microscope system having either one or both of a fluorescence microscope and a total reflection microscope and an objective optical system facing each other with the sample interposed therebetween. The fluorescence observation or fluorescence photometric method according to claim 9. 前記工程Ｂで選択したアプリケーションが動画観察又はタイムラプス観察であることを特徴とする請求項４に記載の蛍光観察又は蛍光測光方法。5. The fluorescence observation or fluorescence photometry method according to claim 4, wherein the application selected in the step B is moving image observation or time lapse observation. 前記工程Ｂで選択したシステムが蛍光顕微鏡システムであることを特徴とする請求項１３に記載の蛍光観察又は蛍光測光方法。The fluorescence observation or fluorescence photometry method according to claim 13, wherein the system selected in the step B is a fluorescence microscope system. 前記工程Ｂで選択したシステムが全反射顕微鏡システムであることを特徴とする請求項１３に記載の蛍光観察又は蛍光測光方法。14. The fluorescence observation or fluorescence photometry method according to claim 13, wherein the system selected in step B is a total reflection microscope system. 前記工程Ｂで選択したシステムが蛍光顕微鏡と全反射顕微鏡のいずれか一方を２つ又は両方を１つずつ有し、前記試料を挟んで対物光学系が対向配置された顕微鏡システムとして構成されていることを特徴とする請求項１３に記載の蛍光観察又は蛍光測光方法。The system selected in the step B is configured as a microscope system having either one or both of a fluorescence microscope and a total reflection microscope and an objective optical system facing each other with the sample interposed therebetween. The fluorescence observation or fluorescence photometry method according to claim 13.
JP2006175494A 2006-06-26 2006-06-26 Fluorescence observation or fluorescence photometry system, and method thereof Abandoned JP2008003051A (en)

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JP2006175494A JP2008003051A (en) 2006-06-26 2006-06-26 Fluorescence observation or fluorescence photometry system, and method thereof
US11/588,646 US7696491B2 (en) 2006-06-26 2006-10-27 Fluorescence observation or fluorescence measuring system, and fluorescence observation or fluorescence measuring method

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JP2006175494A JP2008003051A (en) 2006-06-26 2006-06-26 Fluorescence observation or fluorescence photometry system, and method thereof

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JP2008003051A JP2008003051A (en) 2008-01-10
JP2008003051A5 true JP2008003051A5 (en) 2009-08-13

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JP5365736B1 (en) * 2012-07-24 2013-12-11 富山県 Microwell array chip and cell recovery method

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