JP2007330857A - Apparatus and method for transferring liquid - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a simple-structured apparatus for transferring liquid with a small surface area wherein its flow path, chamber and the like are disposed capable of achieving a complex control of liquid transfer, and a method for transferring liquid. <P>SOLUTION: The apparatus for transferring liquid is constituted of a rotary base body comprised of a rotation shaft 13 and a plurality of chamber chips 17 each disposed in the rotary base body in the radius directions. Each of the chamber chips 17 is constituted of an injection chamber 21, a branched chamber 22, post-branch chambers 23A, 23B, an introduction flow path 24, and post-branch flow paths 25A, 25B. If the rotation of the rotation shaft 13 reaches a certain rotation speed, the centrifugal force exceeds the capillary force to cause the liquid in the injection chamber 21 to flow into a branch chamber 29A of the branched chamber 22 via the introduction flow path 24. The liquid is further supplied from the injection chamber 21 to the branch chamber 29A even after the branch chamber 29A is fully filled with the liquid. The liquid flows into an adjacent branch chamber 29B from the branch chamber 29A through an opening 28 between the top of a barrier wall 22c and a wall surface 22a. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a liquid feeding device and a liquid feeding method. In particular, the present invention relates to a liquid-feeding apparatus and a liquid-feeding method for feeding a liquid by controlling the flow of a small amount of liquid in a microchannel.

  In recent years, various biosensors used as a health check chip for POCT (Point of Care Test) in clinics and homes have been developed. Many of these biosensors are card-type devices having a micro-channel structure called micro-TAS (μ-TAS: Micro Total Analysis System). Patent Document 1 discloses a liquid feeding device using centripetal force or centrifugal force as a technique for quantitatively feeding a microfluid in this type of biosensor or the like. Hereinafter, the liquid feeding device of Patent Document 1 will be described with reference to FIG.

  15 includes a rotatable substrate 1, which includes an inlet 2, chambers 3A, 3B, and 3C, a plurality of metering capillaries 4 that connect the inlet 2 and the chamber 3A, an inlet 2, and the like. An overflow capillary 6 for connecting the overflow chamber 5 and a channel 7 for venting air from the inlet 2 and the chambers 3A to 3B are provided. A liquid such as a liquid sample injected from the inlet 2 flows into the measuring capillary 4 and the overflow capillary 6 by capillary action, and joins the measuring capillary 4 and the first stage chamber 3A, and the overflow capillary 6 and the overflow chamber 5. Reach the junction. When the substrate 1 is rotated at the first rotation speed, excess liquid is introduced into the overflow chamber 5 and the amount of liquid in the metering capillary 4 is quantified. Next, when the substrate 1 is rotated at a second rotational speed larger than the first rotational speed, the liquid in the metering capillary 4 flows into the chamber 3A and further into the chambers 3B and 3C.

  In the configuration disclosed in Patent Document 1, since the liquid is guided to the measuring capillary 4 using the capillary phenomenon in a state where the substrate 2 does not rotate, the permeation induction occurs together with the introduction of the liquid into the inlet 2. . Therefore, the overflow capillary 6 and the overflow chamber 5 are necessary for quantifying the liquid, the structure is complicated, and the arrangement area of the chamber and the like is increased. In addition, with the configuration disclosed in Patent Document 1, it is difficult to realize complicated liquid feeding control such as a combination of determination, branching, and liquid feeding order control.

Japanese Patent No. 3469585

  In view of the above-described conventional problems, an object is to provide a liquid feeding apparatus and a liquid feeding method that have a simple structure, a small arrangement area of a flow path, a chamber, and the like and that can realize complicated liquid feeding control.

  According to a first aspect of the present invention, there is provided a rotatable rotating substrate having a rotation center, an injection chamber provided on the rotating substrate and spatially closed except for an injection port, and the injection chamber of the rotating substrate. Is provided at a position away from the center of rotation, is spatially closed except for the air opening, and has a first wall surface in the centripetal direction, and a second wall surface in the centrifugal direction opposite to the first wall surface, At least one barrier extending from the second wall surface toward the first wall surface, the barrier defining a plurality of branch chambers including an initial branch chamber having a defined volume, wherein the adjacent branch chambers are A branch chamber communicating with each other via a gap between the front end of the barrier and the first wall surface; and a position farther from the center of rotation than the branch chamber of the rotating base, Except for spatially closed branches A first end connected to the injection chamber holds the liquid in the injection chamber by capillary force, and is formed on the rotating base, and is connected to the injection chamber. A second end portion that opens to the branch chamber at the wall surface of 1 is formed in the guide channel facing the initial branch chamber, the rotating base, and connects the branch chamber and the post-branch chamber to form the branch An end portion connected to the chamber provides a liquid feeding device including a post-branch flow path that holds the liquid in the branch chamber by capillary force, and a rotation driving unit that rotates the rotating base around the rotation center. The volume of the initial branch chamber is preferably smaller than the volume of the injection chamber.

  Liquid is supplied from the inlet to the injection chamber. Liquid is held in the injection chamber by the capillary force at the first end of the guide channel. When the rotation driving unit rotates the rotating substrate, centrifugal force acts on the liquid held at the end of the first flow path. When the rotation speed of the rotating substrate reaches a certain speed (first rotation speed) and the centrifugal force exceeds the capillary force, the liquid in the injection chamber flows into the initial branch chamber of the branch chamber through the guide channel. Even after the initial branch chamber is filled with liquid, liquid is further supplied from the injection chamber to the initial branch chamber. The liquid flows from the initial branch chamber to another adjacent branch chamber through a gap between the tip of the barrier and the first wall surface of the branch chamber. In other words, the liquid is supplied from the initial branch chamber to another adjacent branch chamber by overflowing the barrier. As a result, a prescribed volume of liquid is stored at least in the initial branch chamber. The liquid is held in the branch chamber by the capillary force at the end of the post-branch flow path. When the rotational drive unit increases the rotational speed of the rotating substrate to a certain speed (second rotational speed) faster than the first rotational speed described above, the centrifugal force exceeds the capillary force at the end of the post-branch flow path, The liquid in the branch chamber flows into the post-branch chamber through the post-branch flow path.

  The above-described post-branch flow path and post-branch chamber can be provided for each branch chamber. In other words, the individual post-branch flow paths may be connected to the individual branch chambers of the branch chamber, and the post-branch flow paths may be connected to the separate post-branch chambers. With this configuration, the liquid in each branch chamber can be sent and stored in a separate post-branch chamber.

  One common post-branch chamber may be provided for a plurality of branch chambers. That is, the individual post-branch flow paths may be connected to the individual branch chambers of the branch chamber, and the individual post-branch flow paths may be connected to the common post-branch chamber. With this configuration, the liquid once branched into the individual branch chambers in the branch chamber can be sent to the common post-branch chamber in a desired order.

  An additional chamber provided at a position farther from the center of rotation than the post-branch chamber of the rotating base and closed except for an air port, and the post-branching chamber and the additional chamber formed in the rotating base. And an end connected to the post-branch chamber may further include an additional flow path for holding the liquid in the branch chamber by capillary force.

  The second aspect of the present invention is an injection chamber that is spatially closed except for the injection port, and is provided at a position farther from the center of rotation than the injection chamber, and is spatially closed except for the air port, A centripetal first wall, a centrifugal second wall facing the first wall, and at least one barrier extending from the second wall toward the first wall; The barrier defines a plurality of branch chambers including an initial branch chamber having a defined volume, and the adjacent branch chambers communicate with each other via a gap between the tip of the barrier and the first wall surface. A branch chamber, a post-branch chamber which is provided at a position farther from the rotation center than the branch chamber and is spatially closed except for an air port, the injection chamber and the branch chamber are connected, and the injection A first end connected to the chamber A second end that holds the liquid in the injection chamber by capillary force and opens to the branch chamber on the first wall surface is a guide channel facing the initial branch chamber, the branch chamber, and the branch Provided is a rotating base that is connected to a rear chamber, and an end portion that is connected to the branch chamber includes a post-branch flow path that holds a liquid in the branch chamber by capillary force.

  The rotating substrate includes, for example, a substrate body having a rotation center and a plurality of chamber chips that are detachably attached to the substrate body. The chamber chip is provided with the above-described injection chamber, branch chamber, post-branch chamber, induction channel, and branch channel. Alternatively, the rotating base may have a structure in which the base body itself includes an injection chamber, a branch chamber, a post-branch chamber, a guide channel, and a branch channel.

  The third aspect of the present invention provides a liquid feeding method including the following steps. First, the aforementioned rotating base is prepared. Next, the liquid is injected into the injection chamber from the injection port. Subsequently, the rotating base is rotated at a first rotation speed, and a centrifugal force exceeding the capillary force is applied to the liquid at the first end of the induction channel, thereby causing the liquid to pass through the induction channel. The liquid is allowed to flow from the injection chamber into the initial branch chamber of the branch chamber. Further, even after the initial branch chamber is full, the liquid supplied from the injection chamber to the initial branch chamber by the rotation of the first rotation speed of the rotating base passes through the gap to the initial branch chamber. To the other adjacent branch chamber. Thereafter, the rotary base is rotated at a second rotational speed that is faster than the first rotational speed, and a centrifugal force that exceeds the capillary force is applied to the liquid at the end of the post-branch channel, thereby The liquid is caused to flow from the branch chamber into the post-branch chamber via the post-branch flow path.

  In the liquid feeding apparatus and the liquid feeding method of the present invention, the liquid supplied from the injection chamber to the initial branch chamber of the branch chamber by the centrifugal force overflows the partition wall and overflows to another adjacent branch chamber, thereby causing the liquid branching. Quantification is realized. Accordingly, the structure of the rotating base is simple in that it is not necessary to provide an overflow capillary or the like necessary for quantification using the capillary phenomenon, and the arrangement area of the flow paths and chambers in the rotating base can be reduced. In addition, there is a high degree of freedom in the arrangement of chambers and flow paths, including the number of branch chambers, the volume of each branch chamber, the number of chambers after branching with respect to the branch chamber, etc. Liquid feeding control can be realized.

(First embodiment)
1 to 3 show a liquid delivery device 11 according to a first embodiment of the present invention.

  The liquid feeding device 11 includes a rotary base 12, a rotary shaft 13 to which the rotary base 12 is fixed, a motor 14 that rotationally drives the rotary shaft 13, and a drive circuit 15 for the motor 14. The rotation shaft 13 is arranged in a posture in which the rotation center C, which is the axis thereof, extends in the vertical direction. The rotating base 12 is driven to rotate by a motor 14 and can rotate in a clockwise direction R1 and a counterclockwise direction R2 in a plan view. In the present embodiment, the rotary base 12 includes a disc-shaped rotary base body 16 and a plurality of chamber chips 17 that are detachably accommodated in the accommodation holes 16 a provided in the rotary base body 16. .

  The chamber chip 17 will be described with reference to FIGS. In the following description, the position and orientation with respect to the rotation center C are based on the state in which the chamber chip 17 is attached to the rotating base body 16. The chamber chip 17 is provided with an injection chamber 21, a branch chamber 22, and a pair of post-branch chambers 23A and 23B. Among these chambers 21, 22, 23 </ b> A, and 23 </ b> B, the injection chamber 21 is disposed closest to the rotation center C in plan view. The branch chamber 22 is provided at a position farther from the rotation center C than the injection chamber 21 in plan view. In other words, the branch chamber 22 is located outside the injection chamber 21 in the radial direction r (see FIG. 1) of the rotating shaft 13. Further, the post-branch chambers 23A and 23B are provided at positions farther from the rotation center C than the branch chamber 22 in plan view. In other words, the post-branch chambers 23 </ b> A and 23 </ b> B are located outside the branch chamber 22 in the radial direction r of the rotating shaft 13. The injection chamber 21 and the branch chamber 22 are connected by a guide channel 24 extending in the radial direction r of the rotary shaft 13. The branch chamber 22 and the post-branch chambers 23A and 23B are connected to each other by separate post-branch flow paths 25A and 25B extending in the radial direction r of the rotating shaft 13, respectively. As will be described in detail later, the liquid injected into the injection chamber 21 branches in the branch chamber 22, and the branched liquid flows into the chambers 23A and 23B, respectively. The liquid moves in the flow path and the chamber by the centrifugal force generated by the rotation of the rotating base 12.

  The injection chamber 21 is formed inside the chamber chip 17 and is spatially closed. In the present embodiment, the injection chamber 21 is a substantially rectangular parallelepiped space, and has a rectangular shape in plan view. The chamber chip 17 is formed with an injection port 26 that penetrates from the top wall of the injection chamber 21 to the upper surface of the chamber chip 17 and communicates the inside of the injection chamber 21 with the outside of the chamber chip 17. An inlet end 24a of the guide channel 24 is opened on the wall surface in the centrifugal direction of the injection chamber 21, that is, the wall surface 21a located outside the radial direction r. The inlet 26 is formed at a position closer to the rotation center C than the inlet end 24 a of the guide channel 24.

  The branch chamber 22 is formed inside the chamber chip 17 and is spatially closed. In the present embodiment, the branch chamber 22 is a substantially rectangular parallelepiped space as a whole, and has a rectangular shape elongated in a direction perpendicular to the radial direction r of the rotation shaft 13 in plan view. The chamber chip 17 is formed with an air port 27 that penetrates from the top wall of the branch chamber 22 to the upper surface of the chamber chip 17 and communicates the inside of the branch chamber 22 with the outside of the chamber chip 17. The air port 27 has a function of discharging the air in the branch chamber 22 to the outside of the chamber chip 17 when the liquid flows into the branch chamber 22. An outlet end portion 24b of the guide channel 24 is opened in a centripetal wall surface of the branch chamber 22, that is, a wall surface (first wall surface) 22a located inside the radial direction r.

  A wall 22c protrudes from the wall surface of the branch chamber 22 in the centrifugal direction facing the wall surface 22a, that is, from the wall surface (second wall surface) 22b located outside the radial direction r toward the wall surface 22a. The barrier 22c is integrally connected to the wall surface 22b at the base end side, and the distal end faces the wall surface 21a with a gap 28 therebetween. The lower end of the barrier 22c is in close contact with the bottom wall of the branch chamber 22 (the upper surface of the lower surface substrate 36 described later), and the upper end of the barrier 22c is in close contact with the top wall of the branch chamber 22 (the lower surface of the upper surface substrate 37 described later). ing. By providing the barrier 22c, the inside of the branch chamber 22 is divided into two branch chambers 29A and 29B adjacent to each other. These branch chambers 29A and 29B communicate with each other through a gap 28 between the tip of the barrier 22c and the wall surface 21a. Referring to FIG. 2, the outlet end 24b of the guide channel 24 opened at the wall surface 22a of the branch chamber 22 is located on the branch chamber (initial branch chamber) 29A side of the barrier 22c in plan view. In other words, the outlet end portion 24 b of the guide channel 24 faces the branch chamber 29 </ b> A in the radial direction r of the rotating shaft 13. The capacity of the branch chamber 29A is precisely defined. Here, the capacity of the branch chamber 29A refers to the maximum volume of liquid that can be stored in the branch chamber 29A without overflowing the barrier 22c and causing the liquid to flow into the branch chamber 29B or overflow. The volume of the branch chamber 29 is set smaller than the volume of the injection chamber 21.

  In the present embodiment, the barrier 22c has a rectangular shape with a substantially constant width (dimension in the direction orthogonal to the radial direction r) in plan view. However, the shape of the barrier 22c ensures a gap 28 between the wall surface 21a and accurately defines the volume of the branch chambers 29A and 29B, particularly the volume of the branch chamber 29A facing the outlet end 24b of the guide channel 24. It is not particularly limited as long as it is done.

  The inlet wall 25a of the after-branch flow paths 25A and 25B is opened in the outer wall surface 22b of the branch chamber 22. Specifically, the inlet end 25a of one post-branch channel 25A opens to the branch chamber 29A, and the inlet end 25a of the other post-branch channel 25B opens to the branch chamber 29B.

  The post-branching chambers 23A and 23B are formed inside the chamber chip 17 and are spatially closed. In the present embodiment, the whole is a flat cylindrical space, and is generally circular in plan view. The chamber chip 17 is formed with air ports 31A and 31B that penetrate from the top walls of the post-branch chambers 23A and 23B to the upper surface of the chamber chip 17 and communicate the inside of the post-branch chambers 23A and 23B with the outside of the chamber chip 17. ing. The air ports 31A and 31B have a function of discharging the air in the post-branch chambers 23A and 23B to the outside of the chamber chip 17 when the liquid flows into the post-branch chambers 23A and 23B. Outlet end portions 25b of the after-branch flow paths 25A and 25B are opened in the centripetal wall surfaces of the after-branch chambers 23A and 23B.

In order for the liquid to be reliably sent from the injection chamber 21 to the branch chamber 22 through the guide channel 24, the guide channel 24 needs to be a fine channel. Similarly, the post-branch flow paths 25A and 25B are fine flow paths so that the liquid can be reliably fed from the branch chamber 22 to the post-branch chambers 23A and 23B through the post-branch flow paths 25A and 25B. There is a need. Specifically, the volumes of the guide channel 24 and the after-branch channels 25A and 25B are preferably equal to or smaller than the volume of the injection chamber 21, the branch chamber 22, and the after-branch chambers 23A and 23B. The width and depth of the guide channel 24 and the after-branch channels 25A and 25B are preferably smaller than the width and depth of the injection chamber 21, the branch chamber 22, and the after-branch chambers 23A and 23B. Preferably, the induction passage 24 and the branch after passage 25A, the cross-sectional area of 25B is at 1 [mu] m ² or more 4 mm ² or less, preferably is a 25 [mu] m ² or more 100000 ² or less, more preferably a 10000 ^2. The effect of retaining the solution in the branch chamber at the time of branching is greater when the cross-sectional area of the guide channel 24 is larger than the cross-sectional areas of the post-branch channels 25A and 25B. However, the present invention is not limited to this, and the cross-sectional area of the guide flow path 24 may be the same as or smaller than the cross-sectional areas of the post-branch flow paths 25A and 25B. The structure for generating the force for starting the liquid feeding is not dependent on the cross-sectional area of the flow path, and the centrifugal force Fg acting on the branch chambers 29A and 29B can be designed by freely designing the width and depth of the chamber (see FIG. (See 5). Details of the liquid feeding method will be described later.

  The inlet end 24a of the guide channel 24 connected to the injection chamber 21 has a function as a valve for releasably holding the liquid stored in the injection chamber 21. Similarly, the inlet end portions 25a of the after-branch flow paths 25A and 25B connected to the branch chamber 22 function as valves that releasably hold the liquid stored in the branch chamber 22 (branch chambers 29A and 29B). Have Hereinafter, this valve function will be described in detail. First, the inlet end portions 24a and 25a are hydrophobic. Referring to FIG. 5, the liquid end of the liquid 32 is caused by the capillary force Fc due to the surface tension by making the inlet end portions 24a and 25a of the guide passage 24 and the post-branch passages 25A and 25B, which are fine passages, hydrophobic. It is held by the end portions 24a and 25a, and the inside of the guide channel 24 and the branched channels 25A and 25B is not wetted by the liquid 32. Since the flow path wall surfaces of the inlet ends 24a and 25a are hydrophobic, the liquid 32 does not get wet and the contact angle θc between the liquid 32 and the flow path wall surface becomes an obtuse angle, so that the liquid 32 is filled in the injection chamber 21 and the branch chambers 22A and 22B. Capillary force Fc is generated in a direction to be held in the tube. Specifically, surface tensions T <b> 1 to Tn are generated at the interface between the flow path wall surface and the liquid 32, and the capillary force Fc, which is a resultant force, is directed inward in the radial direction r (centric direction) of the rotating shaft 13. In other words, the capillary force Fc is generated in a direction from the inlet end portions 24 a and 25 a toward the inside of the injection chamber 21 and the branch chamber 22. The magnitude of the capillary force Fc is expressed by the following formula (1).

  Here, the symbol T represents the surface tension of water, θc represents the contact angle of the liquid 9 with respect to the channel wall surface, and c represents the perimeter of the channel.

  As described above, the wall surfaces of the inlet channels 24a and 25a of the guide channel 24 and the post-branch channels 25A and 25B are hydrophobic, but the guide channel 24 and the post-branch channel 25A including the outlet ends 24b and 25b. , 25B and the walls of the injection chamber 21, branch chamber 22, and post-branch chambers 23A, 23B may be hydrophilic or hydrophobic.

  Referring to FIG. 3, in this embodiment, the chamber chip 17 has a three-layer structure in which a chamber substrate 35, a lower surface substrate 36, and an upper surface substrate 37 are joined in a stacked state. In the chamber substrate 35, an injection chamber 21, a branch chamber 22, and post-branch chambers 23A and 23B are provided so as to penetrate in the thickness direction, and a guide channel 24 and post-branch channels 25A and 25B are formed on the upper surface side. ing. The lower substrate 36 is bonded to the lower surface of the chamber substrate 35. No holes or the like are formed in the lower substrate 36. The upper surface of the lower substrate 36 constitutes the bottom walls of the injection chamber 21, the branch chamber 22, and the post-branch chambers 23A and 23B. In FIG. 3, the three-layer chamber chip 17 including the chamber substrate 35, the lower substrate 36, and the upper substrate 37 is shown as an example, but the stacked structure of the chamber chips 17 is not limited to this. For example, the chamber chip 17 may have a two-layer structure including a chamber substrate 35 and a lower substrate 36 integrated with an upper substrate 37 by forming a flow path and a chamber by cutting. Further, the position of the flow path with respect to the chamber is not limited to that shown in FIG. In FIG. 3, the flow path is formed so as to be in contact with the upper surface substrate 37 of the chamber cross section, but the flow path may be disposed on the lower surface substrate 36 side, and the flow path is near the center of the cross section on the centrifugal side of the chamber. It may be arranged.

  Next, the liquid feeding method using the liquid feeding apparatus 11 of 1st Embodiment is demonstrated. First, as shown in FIG. 4A, the liquid 32 is injected into the injection chamber 21 from the injection port 26, and the inside of the injection chamber 21 is filled with the liquid 32. Due to the capillary force generated at the inlet end 24a having hydrophobicity in the guide channel 24, the liquid 32 is held at the inlet end 24a without entering the guide channel 24 (see FIG. 5).

  Next, the rotating base body 16 is rotated around the rotating shaft 13. By this rotation, the centrifugal force Fg (see FIG. 5) acts on the liquid 32 held by the capillary force at the inlet end 24a. When the rotational speed of the rotating base body 16 reaches a certain speed (rotational speed RV1) and the centrifugal force Fg exceeds the capillary force Fc at the inlet end 24a of the guide flow path 24 (see the above formula (1)), the induced flow The liquid 32 held by the inlet end 24a of the passage 24 is released. As a result, the liquid 32 in the injection chamber 21 flows into the branch chamber 22 through the guide channel 24 as shown in FIG. 4B. As described above, the outlet end portion 24b of the guide channel 24 is provided to face the branch chamber 29A, so that the liquid 32 flowing from the injection chamber 21 into the branch chamber 22 via the guide channel 24 flows into the branch chamber. Enter 29A.

  As described above, the capacity of the branch chamber 29A is set to be smaller than the capacity of the injection chamber 21. Therefore, if the rotation at the rotation speed RV1 is continued, the injection chamber 21 is further filled after the branch chamber 29A is filled with the liquid 32. The liquid 32 is supplied to the branch chamber 21 from the first chamber. As a result, as shown in FIG. 4C, the liquid 32 flows from the branch chamber 29A into the branch chamber 29B through the gap 28 between the tip of the barrier 22c and the wall surface 22a. In other words, the liquid 32 is supplied from the branch chamber 29A to the branch chamber 29B by overflowing over the barrier 22c.

  As shown in FIG. 4D, in a state where all the liquid 32 in the injection chamber 21 is supplied to the branch chamber 22, the branch chamber 29A stores a volume of liquid 32 corresponding to the specified volume, and the branch chamber 29B stores the volume. A volume of liquid 32 corresponding to the difference obtained by subtracting the volume of the branch chamber 29A from the volume of the injection chamber is stored. Centrifugal force acts on the liquid 32 stored in the branch chambers 29A and 29B by the rotation at the rotation speed RV1. However, the liquid 32 in the branch chambers 29A and 29B is held without flowing into the post-branch channels 25A and 25B due to the capillary force generated at the inlet end portions 25a of the post-branch channels 25A and 25B.

  The rotating base body 16 reaches a certain speed (rotational speed RV2) exceeding the above-mentioned rotational speed RV1, and the centrifugal force Fg is the capillary force Fc at the inlet end 25a of the post-branch flow paths 25A and 25B (see the above formula (1) ), The holding of the liquid 32 by the inlet end 25a of the after-branch flow paths 25A and 25B is released. As a result, as shown in FIG. 4E, the liquid 32 in the branch chambers 29A and 29B of the branch chamber 22 flows into the after-branch chambers 23A and 23B through the after-branch flow paths 25A and 25B, respectively. Eventually, the post-branch chamber 23A stores a volume of liquid 32 corresponding to the capacity of the branch chamber 29A of the branch chamber 22, and the post-branch chamber 23B has a difference obtained by subtracting the volume of the branch chamber 29A from the volume of the injection chamber. A liquid 32 having a volume corresponding to is stored. For example, when the volume of the injection chamber 21 is 10 μL and the volume of the branch chamber 29A of the branch chamber 22 is 5 μL, 5 μL of the liquid 32 is supplied to the post-branch chambers 23A and 23B, respectively.

  As described above, the liquid 32 supplied from the injection chamber 21 to the branch chamber 29A of the branch chamber 22 by the centrifugal force overflows the partition wall 22c and overflows to the other adjacent branch chamber 29B. Quantification is realized. Therefore, it is not necessary to provide the rotating base 12 with an overflow capillary or the like necessary for quantification using the capillary phenomenon described with reference to FIG. In this respect, the structure of the rotating base 12 is simple, and the arrangement area of the flow paths and chambers in the rotating base 12 can be reduced. In addition, as shown in various alternatives and other embodiments below, the liquid delivery device of the present invention includes a chamber and flow including the number of branch chambers, the volume of each branch chamber, the number of post-branch chambers relative to the branch chamber, and the like. The degree of freedom of arrangement of the path is high, and complex liquid feeding control combining quantitative, branching, liquid feeding order, etc. can be realized.

  6 to 10 show alternative chamber chips 17. These fluid chips 17 include a single post-branch chamber 23 that is a closed space except for the air port 31. The branch chambers 29A and 29B of the branch chamber 22 are connected to the common post-branch chamber 23 by post-branch flow paths 25A and 25B. Further, these fluid chips 17 are provided with a rear chamber (additional chamber) 41 on the outer side in the radial direction r of the rotating shaft 13 with respect to the post-branch chamber 23 in plan view. The rear chamber 41 is spatially closed except for the air port 42 communicating with the outside of the fluid chip 17. Further, the rear chamber 41 has a rectangular shape elongated in a direction orthogonal to the radial direction r of the rotary shaft 13 in plan view. Further, a rear-stage flow path 43 extending in the radial direction r of the rotary shaft 13 is formed in the fluid chamber 17, and the post-branch chamber 23 and the rear-stage chamber 41 are connected to each other by the rear-stage flow path 43. . One end of the post-stage side channel 43 connected to the post-branch chamber 23 has hydrophobicity so that the liquid in the post-branch chamber 23 can be held by capillary force. Further, in these chamber chips 17, in order to realize a desired liquid feeding sequence, the distance from the rotary shaft 13 to the inlet end portions 24a and 25b of the guide flow path 24 and the post-branch flow paths 25A and 25B, the branch chamber 29A, By setting the width or depth of 29B, the centrifugal force acting on the liquid 32 at the inlet end portions 24a and 25b of the guide passage 24 and the post-branch passages 25A and 25B, and the liquid 32 at the inlet end portions 24a and 25b. The capillary force to hold is adjusted.

  In the chamber chip 17 shown in FIG. 6, the volumes of the injection chamber 21 and the branch chambers 29A and 29B are set to 15 μL, 10 μL, and 5 μL, respectively. In the chamber chip 17 of FIG. 6, the distances from the rotation axis of the inlet ends 25a of the two post-branch flow paths 25A and 25B are set equal. The liquid 32 flows from the injection chamber 21 to the branch chamber 29A of the branch chamber 22 at the first rotation speed (for example, 813 rpm), and the liquid 32 overflows from the branch chamber 29A to the branch chamber 29B. When the overflow is completed, 10 μL and 5 μL of liquid 32 are stored in the branch chambers 29A and 29B, respectively. When the rotational speed increases to the second rotational speed (for example, 979 rpm), 10 μL of the liquid 32 in the branch chamber 29A flows into the post-branch chamber 23 through the post-branch flow path 25A. Further, when the rotational speed is increased to the third rotational speed (for example, 1068 rpm), 10 μL of the liquid 32 in the post-branch chamber 23 flows into the rear chamber 41 through the rear flow path 43. When the rotation speed increases to the fourth rotation speed (for example, 1312 rpm), 5 μL of the liquid 32 in the branch chamber 29B flows into the post-branch chamber 23 through the post-branch flow path 25B. Further, when the rotational speed is increased to the fifth rotational speed (for example, 1502 rpm), 5 μL of the liquid 32 in the post-branch chamber 23 flows into the rear chamber 41 through the rear flow path 43. By this liquid feeding operation, 10 μL of solution and 5 μL of solution can be injected and discharged into the chamber 23 after branching in this order. In these liquid feeding operations, not only the quantitative liquid feeding in the chamber but also the action of sequential reaction can be given. That is, the reaction solution can be sent into the post-branch chamber 23, drained after the reaction, and then another reaction solution can be fed into the post-branch chamber 23. Such a liquid feeding operation is particularly important when a plurality of chemical reactions are performed on the chip. In addition, the reaction solution can be sent to the post-branch chamber 23, drained after the reaction, and then the cleaning solution can be sent to the post-branch chamber 23. These operations are particularly important when an immune reaction such as an antigen-antibody reaction is performed on a chip.

  In the chamber chip 17 shown in FIG. 7, the volumes of the injection chamber 21 and the branch chambers 29A and 29B are set to 15 μL, 10 μL, and 5 μL, respectively. In the chamber chip 17 of FIG. 7, the width of the branch chamber 29B having a small capacity is set sufficiently narrower than that of the branch chamber 29A having a large capacity, and the inlet end 25b of the post-branch flow path 25B connected to the branch chamber 29B is branched. It arrange | positions in the position away from the rotating shaft 13 rather than the inlet end part 25a of the flow path 25A after branching connected to the chamber 29A. The liquid 32 flows from the injection chamber 21 to the branch chamber 29A of the branch chamber 22 at the first rotation speed (for example, 813 rpm), and the liquid 32 overflows from the branch chamber 29A to the branch chamber 29B. When the overflow is completed, 10 μL and 5 μL of liquid 32 are stored in the branch chambers 29A and 29B, respectively. When the rotational speed increases to the second rotational speed (for example, 880 rpm), 5 μL of the liquid 32 in the branch chamber 29B flows into the post-branch chamber 23 through the post-branch flow path 25B. Further, when the rotation speed increases to the third rotation speed (for example, 990 rpm), 10 μL of the liquid 32 in the branch chamber 29A flows into the post-branch chamber 23 through the post-branch flow path 25A. As a result of this inflow, 15 μL of the liquid 32 in the post-branch chamber 23 then flows into the rear chamber 41 through the rear flow path 43 as it is. By this liquid feeding operation, it is possible to inject 5 μL of solution first and then 10 μL of solution into the chamber 23 after branching. In these liquid feeding operations, not only the quantitative liquid feeding in the chamber but also the reaction can be given sequentially. That is, the reaction solution can be fed into the post-branch chamber 23 and another reaction solution can be fed into the post-branch chamber 23 after a predetermined time. These operations are particularly important when performing a multistage enzyme reaction on a chip.

  In the chamber chip 17 shown in FIG. 8, the volumes of the injection chamber 21 and the branch chambers 29A and 29B are set to 15 μL, 5 μL, and 10 μL, respectively. Further, in the chamber chip 17 of FIG. 8, the width of the large-capacity branch chamber 29B is set to be sufficiently wider than the small-capacity branch chamber 29A, so Are set equal to each other. The liquid 32 flows from the injection chamber 21 to the branch chamber 29A of the branch chamber 22 at the first rotation speed (for example, 813 rpm), and the liquid 32 overflows from the branch chamber 29A to the branch chamber 29B. When the overflow is completed, 5 μL and 10 μL of the liquid 32 are stored in the branch chambers 29A and 29B, respectively. When the rotational speed is increased to the second rotational speed (for example, 1044 rpm), 5 μL of the liquid 32 in the branch chamber 29A flows into the post-branch chamber 23 through the post-branch flow path 25A. Further, when the rotational speed increases to the third rotational speed (for example, 1163 rpm), 10 μL of the liquid 32 in the branch chamber 29B flows into the post-branch chamber 23 through the post-branch flow path 25B. As a result of this inflow, 15 μL of the liquid 32 in the post-branch chamber 23 then flows into the rear chamber 41 through the rear flow path 43 as it is. By this liquid feeding operation, 5 μL of solution can be injected first, and then 10 μL of solution can be injected into the chamber 23 after branching. As can be seen in the chamber chip 17 of FIGS. 7 and 8, the liquid feeding can be started from either of the branch chambers 29A and 29B.

  In the chamber chip 17 shown in FIG. 9, the volumes of the injection chamber 21 and the branch chambers 29A and 29B are set to 15 μL, 5 μL, and 10 μL, respectively. In the chamber chip 17 of FIG. 9, the branch chamber 29B having a large capacity is set wider than the branch chamber 29A having a small capacity, and the inlet end portion 25b of the post-branch flow path 25B connected to the branch chamber 29B is used as the branch chamber 29A. It is arranged at a position farther from the rotary shaft 13 than the inlet end 25a of the post-branch flow path 25A connected to. The liquid 32 flows from the injection chamber 21 to the branch chamber 29A of the branch chamber 22 at the first rotation speed (for example, 813 rpm), and the liquid 32 overflows from the branch chamber 29A to the branch chamber 29B. When the overflow is completed, 5 μL and 10 μL of the liquid 32 are stored in the branch chambers 29A and 29B, respectively. When the rotational speed increases to the second rotational speed (for example, 867 rpm), 10 μL of the liquid 32 in the branch chamber 29B flows into the post-branch chamber 23 through the post-branch flow path 25B. Furthermore, when the rotational speed increases to a third rotational speed (for example, 1060 rpm), 5 μL of the liquid 32 in the branch chamber 29A flows into the post-branch chamber 23 through the post-branch flow path 25A. As a result of this inflow, 15 μL of the liquid 32 in the post-branch chamber 23 then flows into the rear chamber 41 through the rear flow path 43 as it is. By this liquid feeding operation, 10 μL of solution can be injected first, and then 5 μL of solution can be injected into the chamber 23 after branching. As can be seen in the chamber chip 17 of FIGS. 8 and 9, liquid feeding can be started from any of the 5 μL solution and the 10 μL solution from either of the branch chambers 29A and 29B.

  In the chamber chip 17 shown in FIG. 10, the volumes of the injection chamber 21 and the branch chambers 29A and 29B are set to 15 μL, 5 μL, and 10 μL, respectively. In the chamber chip 17 of FIG. 10, the width of the branch chamber 29B having a large capacity is set wider than that of the branch chamber 29A having a small capacity, and the inlet end 25b of the post-branch flow path 25B connected to the branch chamber 29B is used as the branch chamber 29A. It is arranged at a position farther from the rotary shaft 13 than the inlet end 25a of the post-branch flow path 25A connected to. The difference from the chamber chip 17 of FIG. 9 is that the width of the branch chamber 29A is slightly wider than that shown in FIG. 9, and the width of the post-branch chamber 23 is narrower than that shown in FIG. It is. The liquid 32 flows from the injection chamber 21 to the branch chamber 29A of the branch chamber 22 at the first rotation speed (for example, 813 rpm), and the liquid 32 overflows from the branch chamber 29A to the branch chamber 29B. When the overflow is completed, 5 μL and 10 μL of the liquid 32 are stored in the branch chambers 29A and 29B, respectively. When the rotational speed increases to the second rotational speed (for example, 867 rpm), 10 μL of the liquid 32 in the branch chamber 29B flows into the post-branch chamber 23 through the post-branch flow path 25B. Further, when the rotational speed is increased to the third rotational speed (for example, 978 rpm), 10 μL of the liquid 32 in the post-branch chamber 23 flows into the rear chamber 41 through the rear flow path 43. When the rotational speed increases to the fourth rotational speed (for example, 1171 rpm), 5 μL of the liquid 32 in the branch chamber 29A flows into the post-branch chamber 23 through the post-branch flow path 25A. When the rotation speed increases to the fifth rotation speed (for example, 1372 rpm), 5 μL of the liquid 32 in the branch chamber 29 </ b> A flows into the rear chamber 41 through the rear flow path 43. In this liquid feeding operation, the solution first fed to the post-branch chamber 23 is subsequently fed to the rear chamber 41. The first solution fed to the branch chamber 23 and the post-branch chamber 23 thereafter. The solution to be fed to the first chamber may be collected and fed to the latter chamber 41.

  FIG. 11 shows an alternative regarding the structure of the rotating base 12. In this alternative, the rotary base body 16 has a three-layer structure as shown in FIG. 3, for example, and the rotary base body 16 itself includes an injection chamber 21, a branch chamber 22, post-branch chambers 23A and 23B, a guide channel 24, and a post-branch. Channels 25A and 25B are provided.

(Second Embodiment)
The second embodiment of the present invention shown in FIG. 12 is an example in which the chamber chip 17 is configured as a biosensor that electrochemically measures the concentration of CRP (C-reactive protein), which is a kind of antigen contained in serum. is there.

  The volume of the injection chamber 21 of the chamber chip 17 is set to 13 μL. The branch chamber 22 includes two barriers 22c and 22d, and is divided into three branch chambers 29A, 29B, and 29C. The capacities of these branch chambers 29A, 29B, and 29C are set to 5 μL, 5 μL, and 3 μL, respectively. The three branch chambers 29A, 29B, and 29C are connected to a common post-branch chamber 23 by separate post-branch channels 25A, 25B, and 25C. Further, the outlet end of the guide channel 24 faces the central branch chamber 29B among the three branch chambers 29A, 29B, 29C (the branch chamber 29B functions as the initial branch chamber in the present invention). The inlet end portion 25b of the post-branch channel 25B connected to the branch chamber 29B is disposed at a position closer to the rotating shaft 13 than the inlet end portion 25a of the post-branch channel 25A connected to the branch chamber 29A. Further, the inlet end portion 25c of the post-branch channel 25C connected to the branch chamber 29C is disposed at a position closer to the rotating shaft 13 than the inlet end portion 25b of the post-branch channel 25B. In addition to the position of the inlet end portion 25b of the post-branch flow paths 25A to 25C, the setting of the chamber width of the branch chambers 29A, 29B, and 29C increases the rotational speed of the rotary substrate body 16 (see FIG. 1). Thus, the liquid in the branch chambers 29A, 29B, and 29C is supplied to the chamber 23 after branching. A post-stage chamber 41 similar to the alternative shown in FIGS. 6 to 10 is provided outside the post-branch chamber 23, and the post-branch chamber 23 and the post-stage chamber 41 are connected by a post-stage side channel 43. A partition wall 41a protrudes from the centrifugal wall surface of the rear chamber 41 toward the centripetal wall surface. The rear chamber 41 is divided into a detection chamber 44 and a discard chamber 45 by the partition wall 41a. The discarding chamber 45 is opposed to the outlet end portion of the rear stage side channel 43.

  A reagent is carried in the injection chamber 21. The post-branch chamber 23 carries a CRP (hereinafter referred to as an adherent CRP 51A) and an ALP (alcal phosphatase) labeled antibody 53. Further, an electrochemical detection oxidation-reduction body 54 is carried in the post-branch channel 25C. Furthermore, a measurement electrode 55 is arranged in the detection chamber 44 of the rear chamber 41.

  Hereinafter, the procedure for measuring the CRP concentration will be described. First, serum 56 containing CRP 51B to be measured is injected into the injection chamber 21 from the injection port 26. By being held by capillary force at the inlet end of the guide channel 24, the serum 56 is held in the injection chamber 21 (FIG. 12). Next, the rotary base body 16 is rotated around the rotation axis 13 in the clockwise direction R1 at a first rotation speed RV1 (for example, 868 rpm). Due to the centrifugal force generated by this rotation, the serum 56 in the injection chamber 21 flows into the branch chamber 29B of the branch chamber 23 through the guide flow path 24, passes over the barriers 22c and 22d from the branch chamber 29B, and is adjacent to the branch chamber 29A. , 29C (FIG. 13A). When the overflow is completed, 5 μL, 5 μL, and 3 μL of serum 56 are accommodated in the branch chambers 29A, 29B, and 29C, respectively.

  Next, the rotational speed is increased to a second rotational speed RV2 (for example, 1054 rpm), and the serum 56 in the branch chamber 29A is caused to flow from the post-branch flow path 25A into the post-branch chamber 23 (FIG. 13B). A competitive reaction occurs in the branch chamber 23. Specifically, ALP-labeled antibody 53 competitively binds to CRP51B and fixed CRP51A of serum 56. The rate at which ALP-labeled antibody 53 binds to adherent CRP51A depends on the concentration of CRP51B in serum 56. ALP-labeled antibodies 53 include those that bind to CRP51B in serum, those that bind to fixed CRP51A, and those that do not bind to any CRP51A or B.

  After the competitive reaction, the rotational speed is increased to a third rotational speed RV3 (for example, 1123 rpm), and the serum 56 in the post-branch chamber 23 is caused to flow from the rear-stage side flow path 43 into the rear-stage chamber 41 (FIG. 13C). Since the rear-stage flow path 43 is directed to the discard chamber 45, the serum 56 flows into the discard chamber 45 on the right side in the figure. The serum flowing into the discarding chamber 45 includes an ALP-labeled antibody 53 bound to CRP51B in the serum and an ALP-labeled antibody 53 not bound to CRP51. On the other hand, the adhering CRP 51A and the ALP labeled antibody 53 remain in the post-branch chamber 23. The serum solution at this rotational speed RV3 has a function of separating the ALP-labeled antibody 53 bound to the adherent CRP51A and the ALP-labeled antibody 53 released from the adherent CRP51A.

  Subsequently, the rotational speed is increased to a fourth rotational speed RV4 (for example, 1254 rpm), the serum 56 in the branch chamber 29B is caused to flow from the post-branch flow path 25B to the post-branch chamber 23, and further from the branch chamber 23 to the downstream side stream. It flows into the discarding chamber 45 of the rear chamber 41 through the path 43 (FIG. 13D). The feeding of serum 56 at this rotational speed RV4 has a function of washing the fixed CRP 51A in the chamber 23 after branching.

  After cleaning, the rotational speed is increased to a fifth rotational speed RV5 (eg, 1391 rpm). By the rotation at the rotation speed RV5, the serum 56 in the branch chamber 29C flows into the post-branch chamber 23 from the post-branch channel 25C (FIG. 13E). The electrochemical detection redox material 54 carried in the post-branch channel 25 </ b> C flows into the post-branch chamber 23 together with the serum 56. An oxidation-reduction reaction occurs between the ALP-labeled antibody 53 bound to the fixed CRP 51A and the reductant 54 for electrochemical detection.

  Next, the rotational speed is increased to a sixth rotational speed RV6 (for example, 1443 rpm), and the serum 56 in the post-branch chamber 23 is caused to flow into the rear chamber 41 from the rear stage flow path 43 (FIG. 13F). The serum 56 passes through the partition wall 41a and flows into the detection chamber 44 on the left side in the drawing. The amount of ALP-labeled antibody 53 bound to fixed CRP51A can be measured by an electrochemical reaction between electrode 55 and serum 56 (depending on the concentration of CRP51B contained in serum 56 as described above). .

  Since other configurations and operations of the second embodiment are the same as those of the first embodiment, the same elements are denoted by the same reference numerals and description thereof is omitted.

(Example)
The chamber chip 17 according to the first embodiment of the present invention shown in FIGS. 1 to 3 was actually manufactured and a liquid feeding operation was performed.

  First, the manufacture of the chamber chip 17 will be described. A mold made of steel material is produced in which the protruding portions corresponding to the injection chamber 21, the branch chamber 22, the post-branch chambers 23A and 23B, the guide passage 24, and the post-branch passages 25A and 25B are formed by cutting in the cavity. did. The chamber substrate 36 was manufactured by injection molding of urethane resin using this mold. The volumes of the branch chambers 29A and 29B of the branch chamber 22 were both set to 5 μL. The guide channel 24 and the post-branch channels 25A and 25B have a width of 200 μm and a depth of 35 μm. A lower substrate 36 made of PET (polyethylene terephthalate) was joined to the lower surface of the chamber substrate 36 made of urethane resin. Furthermore, an upper surface substrate 37 made of a resin adhesive sheet was bonded to the upper surface of the chamber substrate 36. The top substrate 37 was formed with an inlet 26 and air ports 27, 31A, 31B.

  For comparison, a chamber chip having the same structure as the chamber chip 17 of the first embodiment was manufactured by the same method, except that the barrier 22c was not provided in the branch chamber 22 as shown in FIG. In the following description, reference numeral 17A is used when referring to the chamber chip of the first embodiment, and reference numeral 17B is used when referring to the chamber chip of the comparative example that does not have the barrier 22c.

  Next, the liquid feeding operation will be described. A liquid 32 obtained by mixing a predetermined amount of the sample solution and the reagent was injected into the injection chamber 21 of the chamber chips 17A and 17B (see FIG. 4A). Next, when the rotating base body (see FIG. 1) on which the chamber chips 17A and 17B are mounted is rotated at 800 rpm, the liquid 32 flows through the inlet end portion 24a of the guide channel 24 in any of the chamber chips 17A and 17B. Retained and remained in the injection chamber 21. Subsequently, the rotation of the rotating body base body was continuously increased from 800 rpm at a rate of 20 rpm / second. In both the chamber chips 17A and 17B, the liquid 32 in the injection chamber 21 flows into the branch chamber 22 through the induction flow path 24 by 1620 rpm (see FIG. 4B). The detailed rotation speed at which the liquid flowed out was 1608 rpm for the chamber chip 17A and 1540 rpm for the chamber chip 17B, which were almost simultaneous. As for the chamber chip 17A, the liquid 32 first accumulates only in the branch chamber 29A, and the liquid 32 in the branch chamber 29A moves to the branch chamber 29B through the gap 28 (over the barrier 22c) as the amount of inflow increases ( (See FIG. 4C). The liquid 32 was held in the branch chamber 22 for any of the chamber chips 17A and 17B. In this state, the rotational speed was increased again at the same rate. In any of the chamber chips 17A and 17B, the liquid 32 moved from the branch chamber 22 to the post-branch chambers 23A and 23B before the rotation speed reached 2100 rpm. The detailed rotation speed at which the liquid flows out is as shown in Table 1.

  When the chamber chip 17A is used, the rotational speed at which the flow out of the branch chamber 22 occurs slightly differs between 1990 rpm and 2060 rpm by the above liquid feeding operation. However, after the branch, the chambers 23A and 23B are filled with 5 μL of liquid. It was. In other words, it was confirmed that the liquid 32 supplied to the injection chamber 21 can be branched and quantified. On the other hand, in the chamber chip 17B of the comparative example having no barrier 22c, all the liquid 32 (10 μL) was sent to one post-branch chamber 23A, and the liquid 32 was not supplied to the other branch chamber 23B.

The typical top view showing the liquid sending device concerning a 1st embodiment of the present invention. The top view of the chamber chip of 1st Embodiment. Sectional drawing in the III-III line of FIG. The top view of the chamber chip of the state which supplied the sample solution to the injection | pouring chamber. The top view of the chamber chip currently rotating at the rotational speed RV1 (before overflow). The top view of the chamber chip in overflow. The top view of the chamber chip at the time of completion of overflow. The top view of the chamber chip in the liquid supply to the chamber after a branch. The schematic diagram which shows the capillary force and centrifugal force which act on a liquid. The top view which shows the 1st alternative regarding a chamber chip | tip. The top view which shows the 2nd alternative regarding a chamber chip | tip. The top view which shows the 3rd alternative regarding a chamber chip | tip. The top view which shows the 4th alternative regarding a chamber chip | tip. The top view which shows the 5th alternative regarding a chamber chip | tip. The typical top view which shows the alternative regarding the structure of a rotary base | substrate. The typical top view showing the chamber chip concerning a 2nd embodiment of the present invention ( The typical top view showing the chamber chip concerning a 2nd embodiment of the present invention (at the time of overflow). The typical top view (competitive reaction) which shows the chamber chip concerning a 2nd embodiment of the present invention. The typical top view (separation) showing the chamber chip concerning a 2nd embodiment of the present invention. The typical top view (cleaning) which shows the chamber chip concerning a 2nd embodiment of the present invention. The typical top view (reaction with an enzyme) which shows the chamber chip concerning a 2nd embodiment of the present invention. The typical top view (measurement) which shows the chamber chip concerning a 2nd embodiment of the present invention. The typical top view which shows the chamber chip of a comparative example. The partial top view which shows an example of the conventional liquid feeding apparatus.

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 Liquid feeder 12 Rotating base body 13 Rotating shaft 14 Motor 15 Drive circuit 16 Rotating base body 16a Accommodating hole 17 Chamber chip 21 Injection chamber 21a, 21b Wall surface 22 Branch chamber 22c, 22d Barrier 23, 23A, 23B Post-branch chamber 24 Inductive flow Channel 24a Inlet end 24b Outlet end 25A, 25B, 25C Flow path after branching 25a Inlet end 25b Outlet end 26 Inlet 27, 31, 31A, 31B Air outlet 28 Clearance 29A, 29B, 29C Branch chamber 32 Liquid 35 Chamber substrate 36 Lower surface substrate 37 Upper surface substrate 41 Rear chamber 42 Air port 43 Rear flow channel 44 Detection chamber 45 Discard chamber 51A Adhered CRP
51B CRP to be measured
53 ALP-labeled antibody 54 Redox body for electrochemical detection 55 Electrode 56 Serum C Rotation center

Claims (7)

A rotatable rotating substrate having a center of rotation;
An injection chamber provided on the rotating base and spatially closed except for an injection port;
The rotary base is provided at a position farther from the center of rotation than the injection chamber, is spatially closed except for an air port, and has a first wall in the centripetal direction and a centrifuge facing the first wall. A directional second wall and at least one barrier extending from the second wall toward the first wall, the barrier defining a plurality of branch chambers including an initial branch chamber having a defined volume. The adjacent branch chambers communicate with each other via a gap between the tip of the barrier and the first wall surface;
A post-branch chamber that is provided at a position farther from the rotation center than the branch chamber of the rotating base and is spatially closed except for an air port;
A first end connected to the injection chamber is formed on the rotating base, connects the injection chamber and the branch chamber, holds liquid in the injection chamber by capillary force, and the first wall surface. And the second end opening to the branch chamber has a guide channel facing the initial branch chamber,
A post-branch flow path formed on the rotating base, connecting the branch chamber and the post-branch chamber, and an end connected to the branch chamber holding a liquid in the branch chamber by capillary force;
And a rotation driving unit that rotates the rotating base around the rotation center.   The liquid feeding device according to claim 1, wherein a volume of the initial branch chamber is smaller than a volume of the injection chamber.   2. The separate post-branch channel is connected to each of the branch chambers of the branch chamber, and each of the post-branch channels is connected to the separate post-branch chamber. Liquid feeding device.   2. The feeding device according to claim 1, wherein the individual post-branch flow paths are connected to the individual branch chambers of the branch chamber, and the individual post-branch flow paths are connected to the common post-branch chamber. Liquid device. An additional chamber that is provided at a position farther from the center of rotation than the post-branching chamber of the rotating base and is closed except for an air port;
An additional flow path formed on the rotating base, connecting the post-branch chamber and the additional chamber, and an end connected to the post-branch chamber holding a liquid in the branch chamber by capillary force; The liquid feeding device according to claim 1, further comprising: An injection chamber spatially closed except for the inlet;
It is provided at a position farther from the center of rotation than the injection chamber, is spatially closed except for the air port, and has a first wall in the centripetal direction and a second in the centrifugal direction opposite to the first wall. A wall surface and at least one barrier extending from the second wall surface toward the first wall surface, the barrier defining a plurality of branch chambers including an initial branch chamber having a defined volume, and adjacent to the branch chamber A branch chamber communicates with each other through a gap between the tip of the barrier and the first wall surface;
A post-branch chamber that is provided at a position farther from the center of rotation than the branch chamber and is spatially closed except for an air port;
The injection chamber and the branch chamber are connected, the first end connected to the injection chamber holds the liquid in the injection chamber by capillary force, and opens to the branch chamber at the first wall surface. A second end portion of the guide channel facing the initial branch chamber;
A rotary base comprising: the branch chamber and the post-branch chamber connected to each other, and an end connected to the branch chamber having a post-branch flow path for holding the liquid in the branch chamber by capillary force. An injection chamber spatially closed except for the inlet;
It is provided at a position farther from the center of rotation than the injection chamber, is spatially closed except for the air port, and has a first wall in the centripetal direction and a second in the centrifugal direction opposite to the first wall. A wall surface and at least one barrier extending from the second wall surface toward the first wall surface, the barrier defining a plurality of branch chambers including an initial branch chamber having a defined volume, and adjacent to the branch chamber A branch chamber communicates with each other through a gap between the tip of the barrier and the first wall surface;
A post-branch chamber that is provided at a position farther from the center of rotation than the branch chamber and is spatially closed except for an air port;
The injection chamber and the branch chamber are connected, the first end connected to the injection chamber holds the liquid in the injection chamber by capillary force, and opens to the branch chamber at the first wall surface. A second end portion of the guide channel facing the initial branch chamber;
Connecting the branch chamber and the post-branch chamber, and preparing a rotating base body having an after-branch flow path in which an end connected to the branch chamber holds the liquid in the branch chamber by capillary force;
Injecting the liquid from the inlet into the injection chamber;
The rotary base is rotated at a first rotational speed, and a centrifugal force exceeding the capillary force is applied to the liquid at the first end of the guide channel, thereby causing the injection chamber to pass through the guide channel. The liquid is allowed to flow into the initial branch chamber of the branch chamber from
Even after the initial branch chamber is full, the liquid supplied from the injection chamber to the initial branch chamber by the rotation of the first rotation speed of the rotating base is adjacent to the initial branch chamber through the gap. Flow into the other branching chamber,
The rotary base is rotated at a second rotational speed that is faster than the first rotational speed, and a centrifugal force that exceeds the capillary force is applied to the liquid at the end of the post-branch flow path, thereby the branch. A liquid feeding method, wherein the liquid is caused to flow from the branch chamber into the post-branch chamber via a rear flow path.

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