JP2007286022A - Method for noninvasively and quantitatively measuring antioxidant or other similar substance existing in human skin tissue including mucous tissue such as periodontal tissue or like - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for simply and quantitatively measuring/evaluating an amount of an antioxidant or other nutritional components of a human skin tissue which includes a mucous tissue such as a periodontal tissue or the like, in vivo and noninvasively to a test subject. <P>SOLUTION: A surface of the skin tissue which includes the mucous tissue such as the periodontal tissue or the like of the test subject, is irradiated with visible light, and an obtained absorption spectrum is analyzed by using a multivariate analysis technique, whereby a specific substance such as the antioxidant or the like which is included in the skin tissue irradiated with the visible light can be quantitatively measured. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

Detailed Description of the Invention

In recent years, the involvement of oxidative stress due to active oxygen has been pointed out as one of the causes of various diseases and aging including lifestyle-related diseases such as arteriosclerosis, diabetes, and cancer. Attention has been focused on various antioxidants that protect the body from oxygen attack.

The present invention relates to a method for measuring the abundance of an antioxidant substance or other similar substance in a living body, and is particularly suitable for a method for measuring the abundance of an antioxidant substance or other similar substance in vivo. Technology.

Conventionally, a method of directly quantifying individual antioxidant substances in blood or tissue using a phlegm test or chromatography is generally used as a method for examining the antioxidant power of a living body. Examples of in vivo antioxidants include SOD (superoxide dismutase), catalase, glutathione peroxidase, vitamin E, ascorbic acid (vitamin C), bilirubin, β-carotene and the like. Accordingly, the antioxidant power of a living body is measured by examining the activity or abundance of these individual components in living tissue such as blood.

Problems to be solved by the invention

In the conventional method of examining antioxidant power, it is necessary to collect and analyze the blood, urine, or cells of the subject, so it is not a simple method, and the subject will feel pain and annoyance, There is a problem that it is not a versatile method because it takes both cost and time.

For this reason, there has been a strong demand for the development of methods and methods for measuring and evaluating non-invasive and simple and accurate antioxidant power in vivo.

Means for solving the problem

As a method for solving such a conventional problem, the present invention irradiates visible light from the vicinity of the surface of human skin tissue including mucosal tissues such as periodontal or in contact with the surface, and from the absorption spectrum obtained, Each calibration curve suitable for estimating the abundance of antioxidants or other similar substances in the object is obtained by a variable analysis method, and the absorbance at the wavelength adopted in the calibration curve for the object with an unknown abundance Shows a method for non-invasively determining antioxidant substances or other similar substances present in human skin tissue including mucosal tissues such as periodontal by calculating each abundance using the inspection line from Is.

Visible light means light having a wavelength in the vicinity of 380 to 800 nm. When a living body is irradiated with visible light, the absorption spectrum of scattered light shows a characteristic spectrum attributed to each component existing in the living body. In an object consisting of multiple components, the absorption by each component interferes with each other, and the absorption spectrum is affected in proportion to the amount of each component. Each component content can be measured.

By using visible light, the manufacturing cost is low, it is compact, and it can be used more easily and widely used than any currently used measuring instrument in that it can measure accurately in a very short time of a few seconds. It can be done.

Claims (1)

Irradiate visible light near or in contact with human skin tissue surface including mucosal tissues such as periodontal, etc. Antioxidants or other similar substances in the object by multivariate analysis from the obtained absorption spectrum Each calibration curve suitable for estimating the abundance such as, is obtained, and each abundance is calculated using the inspection line from the absorbance at the wavelength adopted for the calibration curve for an object with an unknown abundance. Non-invasive determination method of antioxidant substances or other similar substances in human skin tissue including mucous membranes such as periodontal by visible light.