JP2007267619A - Tray for cultured tissue and operation unit for tray - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a tray for cultured tissue excellent in handling property in transplantation of tissue culture. <P>SOLUTION: The tray 30 for cultured tissue has a slope-shaped part, a transferring part 40 having an opening part, a placing face 32 having a transparency, a holdable handle for conveyance, a lid body, etc. and the tray 30 is stored in a packaging container. Since the tray 30 for cultured tissue having a placing surface 32 capable of placing a cultured tissue 100 is equipped with a transferring part 40 of the cultured tissue 100, the tray 30 can move the cultured tissue to an any place in a state placed on the tray for cultured tissue while avoiding risk directly holding the cultured tissue or dropping the cultured tissue and then can transfer the cultured tissue from the transferring part 40. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a culture tissue tray used for handling a culture tissue and an operation unit thereof.

  In recent years, a cultured tissue is produced by culturing cells and tissue pieces, and this cultured tissue has been used for research and medical purposes. In general, the cultured tissue is delicate with respect to various conditions, and therefore, it is necessary to minimize the addition of external force to the cultured tissue in addition to controlling the temperature during transportation. In addition, when used for medical purposes, it is necessary to work in a clean environment as much as possible in the trimming, cleaning, and transplantation operations for transplantation.

Various transport containers (packaging containers) have been devised for transporting such cultured tissues. For example, the packaging container described in patent document 1-patent document 5 is mentioned. Patent Document 1 discloses a package of cultured tissue in which a skin wound dressing composed of epidermal cells is held by a substrate sheet and wrapped with a tray and a sheet-like lid. Patent Document 2 discloses a culture tissue package in which a flat membrane-like membrane tissue derived from a living tissue is sandwiched between sheet-like materials, wound into a cylindrical shape, and inserted into a tube. Patent Document 3 discloses a cultured tissue package in which a cultured cell sheet is supported by a support, and is sandwiched between upper and lower net-shaped interposition members and packaged with a tray and a lid. Patent Document 4 discloses a package of cultured tissue in which the entire membranous tissue is held by a protrusion provided on the container main body and the container lid surface. Patent Document 5 discloses a package in which a carrier with an attached tissue equivalent is placed in a tray and packaged with a lid having a depression.
Japanese Patent No. 2733800 JP 2003-009845 A JP 2004-187518 A Japanese Patent Laying-Open No. 2005-047866 Japanese National Patent Publication No. 11-501298

  However, in the package of Patent Document 1 to Patent Document 4, the cultured tissue or the like is directly taken out from the package, washed with another container, and then transplanted to the transplantation wound. There is a risk of dropping cultured tissue and the like when moving to a transplant. Further, in any container, since the side surface of the container is erected from the bottom surface, it is difficult to take out the cultured tissue or the like. Furthermore, in the package of Patent Document 5, by taking out the carrier from the tray, the attached tissue equivalent can also be taken out. However, since the carrier has a shape in which the side faces up from the bottom along the tray shape, Difficult to remove tissue from carrier. Furthermore, since the cultured tissue is attached to the bottom surface of the carrier, it was necessary to perform further peeling work in order to take the cultured tissue. Moreover, the packaging containers described in Patent Document 1 to Patent Document 5 are all package bodies that assume a sheet-like culture tissue, and are not necessarily suitable for transporting a three-dimensional culture tissue. Thus, in any packaging container, it is not considered to take out the cultured tissue after opening the packaging container and carry it or transfer it to the transplanted wound, and it did not assume a three-dimensional shaped cultured tissue. .

  Thus, an object of the present invention is to provide a tray for cultured tissue that is excellent in handleability in transplanting cultured tissue. Another object of the present invention is to provide a tray for cultured tissue that is excellent in handleability when packaging or carrying the cultured tissue. Furthermore, another object of the present invention is to provide a culture tissue tray in which the culture tissue can be easily taken in and out of the packaging container.

  As a result of various studies, the inventors have found that at least one of the above-described problems can be solved by providing a transfer unit for transferring the cultured tissue from the tray to the tray on which the cultured tissue is placed. Was completed. That is, according to the present invention, the following means are provided.

  ADVANTAGE OF THE INVENTION According to this invention, it is a culture tissue tray which has the mounting surface which can mount a culture tissue, Comprising: The culture tissue tray which has the transfer part of the said culture tissue is provided.

  In the cultured tissue tray of the present invention, the export part may have an opening in the side wall of the tray. Moreover, the said transfer part can also have a slope-shaped site | part in the side wall of the said tray.

  In the cultured tissue tray of the present invention, the mounting surface described above can have transparency. Further, at least one through hole may be provided on either the placement surface or the side wall of the tray.

  The culture tissue tray of the present invention can have a gripping conveyance handle. Moreover, the tray for culture tissues of this invention may be an inner tray accommodated in a packaging container provided with a container main body and a lid body that can be applied to the container main body. In this aspect, the tray may have an abutting portion that abuts on the lid body when housed in the packaging container. Further, the tray may have a take-out handle portion that can be gripped when housed in the container body, and the contact portion may be a part of the take-out handle portion. The take-out handle portion may be formed so as to be graspable at an upper central portion of the tray. The take-out handle portion may be formed to be retractable.

  Further, according to the present invention, there is provided a culture tissue tray operation unit, wherein the culture tissue tray is the culture tissue tray according to any one of the above, and a support portion that supports the culture tissue tray. And an operation unit including a tray operation unit having a handle portion that can be gripped.

  The present invention relates to a culture tissue tray having a placement surface on which the culture tissue can be placed, the culture tissue tray having a transfer portion of the culture tissue.

  According to the tray for cultured tissues of the present invention (hereinafter, also simply referred to as “main tray”), the cultured tissue can be placed and provided with a transfer section for transferring the cultured tissue. By providing a transfer unit, avoid the danger of directly gripping or removing the cultured tissue, move the cultured tissue to any place with it placed on the culture tissue tray, and then transfer it from the transfer unit Can do. Therefore, it can be reliably transported and avoid the load on the cultured tissue, and can be supplied to a necessary location, for example, transplanted to a transplanted wound. As a result, a tray for cultured tissue that is excellent in handleability in transplanting the cultured tissue is provided. Further, a culture tissue tray excellent in handling properties such as packaging and carrying of the cultured tissue is provided. This tray can be accommodated in a packaging container for cultured tissue and used as an inner tray. In this case, when the main tray is provided with a take-out handle portion that can be gripped by the container body, a culture tissue tray is provided in which the culture tissue can be easily taken in and out of the packaging container.

  Hereinafter, an embodiment of the present invention will be described with reference to FIG. 4 as an example of an embodiment in which the present tray is used as an inner tray of a culture tissue packaging container.

(Packaging container for cultured tissue)
As shown in FIG. 1, the packaging container 2 includes a container body 4, a lid 20, a tray 30 that is a main tray, and a tray operation unit 50. 4 shows the main tray 30, FIG. 1 shows an outline of the entire packaging container 2, and FIGS. 2 and 3 each contain the tray 30 and the tray operation unit 50 in the container body 4. The cross-sectional view of the state covered with the lid body 20 is shown.

(Container body)
The container body 4 only needs to be formed of a liquid-tight material capable of ensuring the liquid-tightness of a liquid (hereinafter referred to as “storage medium”) for storing and maintaining the cultured tissue. The container body 4 is preferably in a form having an opening 4 a upward from the handling of the cultured tissue 100. The overall shape of the container body 4 can take various forms such as a cylindrical shape and a rectangular box shape.

(Lid)
The lid 20 only needs to be able to cover the opening 4a of the container main body 4 to the extent that the liquid tightness can be secured with respect to the storage medium injected into the container main body 4, and may be formed of a liquid-tight material. preferable. As a mounting form of the lid 20 with respect to the opening 4a of the container body 4, in addition to screwing as shown in FIG. 1, it may be by mechanical coupling depending on the shape such as fitting, or adhesive. It may be through a chemical bonding phase such as welding. When the lid 20 is attached to the container main body 4 by screwing or fitting, the tray 30 or the like will be described later with the attaching operation of the lid 20 to the container main body 4 by screwing or fitting. The operation part 50 can be easily pressed and fixed in the direction of the bottom of the container body 4 and the fixing strength can be easily ensured.

(tray)
The tray 30 can be used as a tray for carrying the cultured tissue 100 independently of the packaging container 2 or performing processing such as washing and trimming. On the other hand, it can be accommodated in the space formed by the container body 4 and the lid 20 and can also be used as an inner tray. Here, the packaging space is formed by covering the opening 4 a of the container body 4 with the lid 20. Therefore, “accommodated in the packaging space” is synonymous with accommodating in the packaging container 2. On the other hand, “accommodated in the container body 4” means that the container body 4 is not covered by the lid body 20 or is accommodated.

  The tray 30 has a placement surface 32 on which the cultured tissue 100 can be placed. The placement surface 32 is not particularly limited as long as the cultured tissue 100 can be placed thereon, and the surface shape, planar shape, and size thereof are not particularly limited. The mounting surface 32 is preferably formed to be approximately flat. Moreover, it is preferable that the entire mounting surface 32 on which the cultured tissue 100 is mounted or at least the region where the cultured tissue 100 is mounted has transparency. By having transparency, for example, after taking out the tray 30 from the packaging container 2, it is placed on a scale display such as a scale or a pattern with a predetermined shape, and the like through the transparent region of the placement surface 32. The scale below can be visually recognized, and the cultured tissue 100 can be cut into a necessary size and shape using such scale. In addition, since the placement surface 32 has transparency, it is easy to confirm the lower state of the tray 30 when the culture tissue 100 is moved to a desired position while the culture tissue 100 is placed on the tray 30. For example, even when the tray 30 is carried to the transplantation site and the cultured tissue 100 is transferred from the transfer portion 40 of the tray 30 and transferred to the transplantation site, the situation around the transplantation site is easily visible and the transplantation operation is performed reliably. Can do. It is preferable that the entire mounting surface 32 has such transparency, more preferably, the side wall 34, particularly the side wall serving as the transfer portion 40, preferably has transparency, and further the entire tray 30. It is preferable to have such transparency.

  A side wall 34 may be provided around the mounting surface 32. The side wall 34 is provided along the periphery of the placement surface 32, but suppresses the culture tissue 100 from falling from the placement surface 32 when the culture tissue 100 is placed and held on the placement surface 32. It is preferable that it is formed at a height and a portion as much as possible. Note that the side wall 34 may not be provided on the entire periphery of the mounting surface 32, or may not have a vertical wall shape rising vertically.

  An example of the side wall 34 of the mounting surface 32 is shown in FIG. The tray 30 shown in FIG. 4 has a side wall 36 having a vertical wall shape and a side wall 38 having a slope shape. Any side wall shape plays a role of preventing the cultured tissue 100 from being released to the outside of the placement surface 32 during normal use.

  The tray 30 can have a transfer unit 40 that can transfer the cultured tissue 100 placed on the placement surface 32 from the tray 30. The transfer unit 40 is a part for transferring the cultured tissue 100 out of the mounting surface 32 when the cultured tissue 100 is transferred from the mounting surface 32 to another container, a transplanted part of the cultured tissue 100, or the like. The transfer unit 40 only needs to have a structure suitable for moving the cultured tissue 100 out of the tray 30. Therefore, for example, it is preferable to form the transfer part 40 by making a part of the side wall 34 of the tray 30 into an opening part and / or a slope-like part. According to these embodiments, the cultured tissue 100 can be easily transferred out of the tray 30 by simply tilting the tray 30. In the form shown in FIGS. 1 and 4, the exporter 40 is formed as a slope-like side wall 38 that the cultured tissue 100 can follow, and in the form shown in FIG. 5A, at the outer peripheral edge of the placement surface. It is formed as an opening portion 37 of the side wall 34. Moreover, as shown in FIG.5 (b), you may have the slope-shaped site | part 39a which points to the downward direction from the opening site | part 37. FIG. Furthermore, as shown in FIG.5 (c), you may make the export part 40 lower than the height of the other side wall 34, and may be formed by the slope-shaped side wall part 39b. Furthermore, as shown in FIG. 5 (d), it may have a slope-shaped side wall 39c whose tip side is narrowed for the transfer of the cultured tissue 100. Providing such a transfer portion 40 on the tray 30 is particularly preferable in a relatively soft cultured tissue that is difficult to grasp with tweezers or the like.

  When the transfer part 40 is an opening part in the side wall 34, the cultured tissue 100 is transferred as it is from the edge of the mounting surface 32, and when the transfer part 40 is the slope part 38 of the side wall 34, it is sloped. The cultured tissue 100 is transferred through the side wall 34. The slope-shaped portion 38 may be a curved surface such as a concave surface, a straight slope, or a fine step shape. In particular, it is preferable to form a slope from the mounting surface 32 to the side wall. Furthermore, appropriate irregularities may be formed on the surface of the export part 40 so that the export of the cultured tissue 100 can be controlled. For example, the concavo-convex portion may be formed in a vertical streak shape or a dot shape along the direction in which the cultured tissue 100 is transferred. By providing a convex guide along the export direction, the export direction can also be regulated. Further, an appropriate surface treatment may be performed so that the cultured tissue 100 is not slippery or slippery. For example, treatments such as increasing hydrophilicity or increasing water repellency can be mentioned.

  It is preferable that at least one such export unit 40 is provided, but it may be provided at two or more locations.

  The tray 30 can have a through hole 42. By having the through-hole 42, it is possible to easily inject / discharge liquids such as a cleaning liquid and a storage medium to the tray 30 in a state where the cultured tissue 100 is mounted on the mounting surface 32. Specifically, when the tray 30 is immersed in the container body 4 filled with the storage medium, the storage medium can be smoothly immersed without resistance by the penetration of the storage medium from the through hole 42. Conversely, when taking out from the storage medium, the storage medium can be easily moved upward by being discharged from the through hole 42. In addition, when the tray 30 is used as an inner tray, the tray 30 can be smoothly moved up and down when the tray 30 is taken in and out of the storage medium filled in the container body 4, and in a packaged state. The preservation medium in the container body 4 can be distributed to the cultured tissue on the tray 30. Furthermore, when the removed tray 30 is placed on a container such as a petri dish and a cleaning solution is injected into the tray 30, the cultured tissue 100 is cleaned and the cleaning solution is discharged from the through hole 42 as needed. Although the formation location of the through-hole 42 is not specifically limited, it can be provided in the mounting surface 32, the side wall 34, or the like. When the through hole 42 is in the vicinity of the bottom of the mounting surface 32 or the side wall 34, it is easy to enter and discharge the liquid, and particularly when the tray 30 is taken out from the container body 4 filled with the storage medium. It is possible to effectively avoid the liquid remaining on the surface 32. 4 shows the through hole 42 formed in the vicinity of the bottom of the tray, and FIGS. 5A, 5B and 5E show the through hole 42 formed in the side wall 34. FIG.

  The tray 30 is preferably formed so as to be supported by the support unit 60 of the tray operation unit 50. By forming the tray 30 so as to be operable by the operation unit 50, an opportunity for the operator to touch the tray 30 itself can be reduced, and handling errors can be reduced. As will be described later, the support portion 60 preferably has a configuration for supporting the tray 30 from below. In particular, in order to enable or ensure such support, it is preferable that the lower surface of the tray 30 has an engagement portion 48 that can be easily locked to the support portion 60. For example, as shown in FIG. 6, the engaging portion 48 can be formed as a convex portion that protrudes downward so that the tray 30 is not displaced relative to the ring-shaped support portion 60.

  When the tray 30 is used as an inner tray, the shape of the tray 30 may be substantially the same as the horizontal sectional shape of the portion where the tray 30 is located when the tray 30 is accommodated in the container body 4. However, it is preferable that at least a part of the tray 30 communicated with each other has an outer peripheral shape separated from the inner wall of the container body 4. That is, in at least a part of the tray 30, the mounting surface 32 and the side wall 34 have an outer peripheral shape separated from the inner wall of the container body 4, so that when the tray 30 is set on the container body 4, A space 35 for liquid passage can be formed between the tray 30 and the liquid can be satisfactorily passed through the tray 30 and in the mounting surface 32, and the tray 30 can be removed from the container body 4 as will be described later. The degree of freedom in the layout of the easy contact portion 46 is increased. Furthermore, it is possible to secure a space through which a part of a tray operation unit 50 described later can be inserted. There may be at least one such separated portion, but preferably there are two or more, and more preferably, the tray 30 is provided symmetrically.

  For example, the tray 30 shown in FIG. 4 has a substantially rectangular outer peripheral shape inserted into the container body 4 having a circular cross section, and can be used as an inner tray of the packaging container 2. That is, the slope-shaped side wall 38 of the peripheral side walls 34 of the substantially circular mounting surface 32 has an arc shape, and the vertical wall-shaped side wall 36 is formed in a straight line shape away from the inner wall of the container body 4. Has been. Therefore, as shown in FIG. 3, a liquid passing space 35 is formed between the vertical side wall 36 and the container body 4. Note that the portion of the tray 30 that is separated from the inner wall of the container main body 4 is only required to form a space between the inner wall and the inner wall. In addition to the form shown in FIG. You may form in.

  When the tray 30 is used as an inner tray, the tray 30 is preferably formed so as to be held at a predetermined portion of the container body 4. The tray 30 may be placed and accommodated on the bottom of the container body 4, but considering the ease of taking out from the container body 4, the tray 30 is closer to the opening 4 a than the vicinity of the bottom. It is preferable that it is held. In order to hold the tray 30 other than the bottom of the container main body 4, a holding portion for holding the tray 30 by engagement or engagement of the tray 30 can be provided on the inner wall of the container main body 4 or the edge of the opening 4 a.

  In FIGS. 1 and 6, as an example of the holding structure of the container body 4 of the tray 30, the inner wall of the container body 4 at the position where the tray 30 is held is formed into a drawn shape so that the slope-shaped side wall 38 of the tray 30 is formed. A configuration in which the lower surface side is held is described. In particular, as shown in FIG. 6, if the receiving portion 8 is formed by cutting out the inner wall of the container body 4 and the engaging portion 44 that locks the receiving portion 8 is provided also on the tray 30 side, 30 can be stably held. Further, as another example, a receiving portion projecting inwardly on the inner wall of the container body 4 is formed, or a part of the upper edge of the tray 30 is locked to the edge of the opening 4a of the container body 4 Possible locking pieces can also be provided. In consideration of mounting and removal of the tray 30 on the container body 4, a holding structure that supports the tray 30 from below is preferable.

  When the tray 30 is used as an inner tray, the tray 30 can include an abutting portion 46 that can abut against the lower surface of the lid 20 when the lid is applied. The abutting portion 46 is abutted and pressed by the lid body 20, whereby the tray 30 can be stably held in the receiving portion 8. The shape of the contact portion 46 is not particularly limited as long as the contact portion 46 is formed so as to be directed upward from the tray 30 and contact the lower surface of the lid 20. In particular, it is preferable to direct upward from a part of the outer peripheral edge of the tray 30. Further, since the contact portion 46 is formed so as to be directed upward from the tray 30, it can also function as a handle portion when the tray 30 is carried alone. The tray 30 shown in FIG. 4 has a tab-like contact portion 46 that protrudes upward from the side wall 36 having a vertical wall shape of the tray 30.

(Tray control unit)
Next, a tray operation unit (hereinafter also simply referred to as an operation unit) 50 will be described. The operation unit 50 is for accommodating the tray 30 in the container body 4, taking it out, or carrying it. The operation unit 50 includes one or two or more handle units 52 that are gripped (including sandwiched and locked) by fingers, tweezers, a robot, or the like. The operation unit 50 only needs to be able to operate the tray 30 and may be provided integrally with the tray 30, but may be separate from the tray 30. When the tray 30 is used as an inner tray of the packaging container 2, it is preferably formed so as to be accommodated in the packaging container 2.

  The operation part 50 in the packaging container 2 shown in FIG. 1 is formed separately from the tray 30, has a support part 60 that supports the tray 30 from below, and has a handle part 52 connected to the support part 60. ing. The form of the support part 60 is not particularly limited as long as the tray 30 can be supported and carried, and the tray 30 can be supported in a form such as locking, fitting, placement, and clamping. If it is formed so as to be supportable from below, it is easy to handle.

  When the tray 30 is used as an inner tray, the support portion 60 can easily release the support state of the tray 30 so that the tray 30 can be easily attached to and detached from the predetermined portion when the tray 30 is put in and out. Is preferred. The tray 30 is preferably fixed to a predetermined site in the container body 4 from the viewpoint of operability. In addition, the holding structure of the tray 30 in the container body 4 and the operation unit 50 itself can be obtained by holding the tray 30 at a predetermined position in the container body 4 even when the tray 30 is not supported by the support part 60 at the time of storage. The degree of freedom of the holding structure is improved. From such a viewpoint, it is preferable that the support state of the tray 30 is easily released, and the support portion 60 preferably supports the tray 30 from below by locking or placing. The tray 30 is supported from below, so that the tray 30 can be stably supported and the support portion 60 can be stored on the lower side of the container body 4, so that the storage form of the cultured tissue 100 is not limited. The space below the container body 4 can be used effectively.

  As the support portion 60 that supports the tray 30 by placing or locking it from below, a frame shape such as a ring shape shown in FIG. 1 or a form such as a tray shape, a net shape, or a sheet shape can be adopted. Considering the resistance of movement and the like in a state where the container body 4 is filled with a liquid, it is preferable to have a frame shape or a net shape.

  When the tray 30 is used as an inner tray, the support portion 60 configured to support the tray 30 from below is preferably formed so as to be placed on the bottom portion of the container body 4 when accommodated in the packaging container 2. As will be described later, when a part of the operation unit 50 is pressed and fixed by the lid body 20, the support unit 60 is placed on the bottom, so that the operation unit 50 is stably held in the packaging container 2. be able to. In addition, you may make it provide the part which supports the support part 60 in the container main body 4 separately from a bottom part, and may support it in the vicinity of a bottom part or other than a bottom part. Moreover, it is preferable that the support part 60 has an external shape which the movement on a bottom part is suppressed by the inner wall (preferably inner wall of a bottom part) of the container main body 4. FIG. By doing so, the accommodation position of the support part 60 in the container main body 4 can be positioned, and the support part 60 and thus the operation part 50 can be held by the inner wall of the container main body 4.

  The operation unit 50 may include a handle unit 52 connected to the support unit 60. The handle 52 is not particularly limited as long as it can be operated by gripping, locking, or pinching with fingers, tweezers, a robot, or the like. Therefore, it can be a small piece such as a tab or a knob, a stick, an arm, a ring, an arch, or the like. The handle portion 52 can function as a tray transport handle portion so that at least the tray 30 can be carried.

  When the tray 30 is used as an inner tray, the handle portion 52 can also function as a handle portion for taking out the tray 30 from the container body 4. The container body 4 is preferably disposed at a position away from the inner wall of the container body 4 so as to avoid or suppress interference with the inner wall of the container body 4 and be held with tweezers. For example, a portion of the stick-like body that extends upward or obliquely upward from the support portion 60 and is separated from the inner wall of the container body 4 can be used as the handle portion 52. Further, when the container body 4 is bent or bent so as to be gripped by a portion near the center of the container body 4, the tray 30 can be stably supported. For example, the handle portion 52 can be a portion that is directed upward from the support portion 60 and projects in a cantilever shape toward the center of the container body 4. Furthermore, in consideration of the storage property of the container body 4, it is also preferable that the tray 30 is movable or retractable so that it can be easily gripped or gripped only when necessary.

  When the handle portion 52 functions as a handle for taking out, as shown in FIG. 1, the handle portion 52 extends upward from the support portion 60 and is further bent to the center side of the container body 4. It is a front-end | tip, Comprising: It can be provided in the center part of the container main body 4 above the tray 30. FIG. That is, in the operation unit 50 shown in FIG. 1, the handle unit 52 is provided above the support unit 60 by a predetermined distance so that the handle unit 52 is positioned above the tray 30 when stored. Moreover, the handle | steering-wheel part 52 can also be provided as a stick shape orient | assigned upwards from the support part 60, as shown to Fig.7 (a). Moreover, as shown in FIG.7 (b), it can also provide in the arm shape curved to the center side of the container main body 4. FIG. Furthermore, as shown in FIG.7 (c), it can be set as the arch shape which straddles the tray 30. FIG. In any of these forms, it can be made into a retractable type in consideration of the accommodation in the packaging container 2. FIG. 7D shows a state in which the arch portion of the arched handle in FIG. 7C is formed to be retractable with respect to the tray 30 and is folded.

  When the tray 30 is used as an inner tray, the operation unit 50 preferably has a holding structure for holding the operation unit 50 itself in the packaging container 2. For example, the operation unit 50 can include a contact portion 54 that contacts the lower surface of the lid 20 when the operation unit 50 is accommodated in the container body 4 and covered. The contact part 54 may be a part of the support part 60 or the handle part 52, or may be the handle part 52 itself. By doing so, the operation unit 50 itself is stably held in the packaging container 2 by the lid. When the support 60 is placed on the bottom of the container body 4, the support 60 is more stably held by the bottom of the container body 4 and the lid 20. The operation unit 50 shown in FIG. 1 uses the handle portion 52 as the contact portion 54 so that the upper end of the handle portion 52 is brought into contact with the lower surface of the lid 20 when the lid is applied. It should be noted that any of the handle portions 52 shown in FIG.

  The tray operation unit 50 includes a tray pressing unit 56 that can press at least a part of the upper edge of the tray 30 when the tray 30 is stored in a predetermined position by the receiving unit 8 of the container body 4. Is preferred. By doing so, when the lid body 20 is mounted on the container body 4 and the abutting portion 54 is pressed by the lower surface of the lid body 20, the tray 30 is surely secured to the receiving portion 8 via the tray pressing portion 56. It will be pressed. As a result, the tray 30 is reliably and stably supported by the receiving portion 8. Such a tray pressing portion 56 can be provided in a part of the regulating portion 90 of the tray operation portion 50 as shown in FIGS. 3, 9B, 10B, and 11B. The tray pressing portion 58 may be a concave portion that can accommodate a part of the upper edge of the tray 30. By setting it as a concave part, the tray 30 can be stably pressed against the receiving part 8 of the container body 4. Further, the tray pressing portion 56 is preferably provided at a position facing the contact portion 46 provided on the tray 30. That is, the tray 30 can be held in the receiving portion 8 more stably by pressing the upper end edge on the opposite side of the contact portion 46 of the tray 30. Further, as shown in the figure, the tray pressing portion 56 is formed in a concave shape with respect to a regulating portion 90 described later, and the upper end edge of the tray 30 is fitted, so that the horizontal movement of the tray 30 against the swing during transportation is achieved. Can be effectively suppressed.

  In addition, the tray operation part 50 demonstrated above is corresponded to the operation unit used for the tray for culture tissues of this invention.

(Regulation Department)
The packaging container 2 can be provided with a regulating portion 90 that can suppress the upward movement of the cultured tissue 100 on the placement surface 32 of the tray 30 to be accommodated. It is preferable that the restricting unit 90 shields at least the upper surface of the placement surface 32 and restricts the vertical movement range of the cultured tissue 100 to the height range of the placement surface 32. Therefore, it may be formed only above the placement surface 32 to restrict vertical movement, or may be formed like a lid that completely shields the opening of the tray 30. The shielding state of the tray 30 by the restricting portion 90 only needs to be formed at least during packaging.

  As shown in FIG. 1, the restricting portion 90 can be provided integrally with the operation portion 50. Such a restricting portion 90 can be formed in various forms with respect to the operation portion 50, but as shown in FIG. 1, it can be provided integrally with a handle portion 52 provided above the tray 30 during packaging. . By doing so, the handle portion 52 can be stably supported. Further, when the handle portion 52 also serves as the contact portion 54, the restricting portion 90 is pressed and fixed to the tray 30 side by the lid body 20 via the contact portion 54, and the operation portion 50 and the restricting portion 90. Is stably held at a predetermined position, and the tray 30 can be stably held by the receiving portion 8 at the same time. In addition, according to such a configuration, the tray 30 is shielded by the restricting portion 90 during packaging, but when the tray 30 is taken out, the operating portion 50 is moved upward by holding the handle portion 52 of the operating portion 50. Then, the regulating portion 90 is relatively moved upward from the tray 30 and the tray 30 is opened. And after taking out, since the control part 90 exists in the upper part fully away from the tray 30, the control part 90 does not become obstructive and subsequent operation becomes easy.

  In addition, the control part 90 can also be made into another form. For example, you may make it restrict | limit only the upper direction of the mounting surface 32 instead of the whole tray 30. FIG. Further, as shown in FIG. 8A, the tray 30 may be opened and closed by rotating the restricting portion 90 in the horizontal direction around a suitable member such as the handle portion 52 as a rotation center. Further, as shown in FIG. 8B, the tray 30 may be formed in a box-like body that can be taken in and out in the horizontal direction. Further, the restricting portion 90 can be provided separately from the operation portion 50. For example, a part (lower surface) of the lid 20 may be used. When the abutting portion 46 is provided at the periphery of the tray 30 or is retractable, the lower surface of the lid 20 is not in the tray 30 when the lid 20 is covered. By forming the packaging container 2 so as to shield the opening, the restricting portion 90 can be configured without additional members.

  One such restricting portion 90 is provided to ensure the reflux of the storage medium filled in the container body 4 and the operability of the restricting portion 90, and to eliminate bubbles in the tray 30 restricted by the restricting portion 90. It is preferable to have the above through holes 92. Moreover, as long as the control part 90 can suppress the upward movement of the culture | cultivation structure | tissue 100, it can also be made into the shape of a net | network or nonwoven fabric which can permeate | transmit a liquid. In addition, the restricting portion 90 does not necessarily have the through hole 92. For example, if there is an appropriate space between the upper edge of the tray 30 and the restricting portion 90, the recirculation property and the bubble discharge property are ensured. Can do. For example, the lower surface of the restricting portion 90 is intentionally formed obliquely so that the upper end edge of the tray 30 and a part of the restricting portion 90 do not come into contact with each other so that a gap is generated between them. Good. Further, the outer peripheral shape of the restricting portion 90 may be smaller than the opening of the tray 30.

  The tray operation unit 50 includes a tray pressing unit 58 that can be pressed by at least a part of the upper end edge of the tray 30 when the tray 30 is stored in a predetermined position by the receiving unit 8 of the container body 4. Is preferred. By doing so, when the lid body 20 is mounted on the container body 4 and the abutting portion 54 is pressed by the lower surface of the lid body 20, the tray 30 is surely secured to the receiving portion 8 via the tray receiving portion 58. It will be pressed. As a result, the tray 30 is reliably and stably supported by the receiving portion 8. Such a tray pressing part 58 can be provided in a part of the regulation part 90 of the tray operation part 50 as shown in FIG. The tray pressing portion 58 may be a concave portion that can accommodate a part of the upper edge of the tray 30. By setting it as a concave part, the tray 30 can be stably pressed against the receiving part 8 of the container body 4.

  Next, a method of packaging the cultured tissue 100 using the tray 30, a package 200 obtained by packaging the cultured tissue 100 in the packaging container 2, and further, taking out the cultured tissue 100 from the package 200 using the tray 30. Will be described. In addition, about the preparation method of a culture tissue, since there is little relationship with this invention, description is abbreviate | omitted.

(Packaging method)
Hereinafter, the packaging method will be described with reference to FIGS. The operator prepares the tray 30 by placing the tray 30 on a clean table so that the lower surface of the tray 30 is in contact with the table, and the operation unit 50 and the container body that are mounted so that the support unit 60 is in contact with the table. 4 and the lid 20 are prepared. Thereafter, the prepared cultured tissue 100 is peeled off from the culture container with a spatula or the like, the peeled cultured tissue 100 is scooped up with a spatula, and gently placed on the mounting surface 32 of the tray 30 so that the surface in contact with the culture container is downward. Placed on.

  After placing the cultured tissue 100 on the placement surface 32, the operator picks up the tray 30 with tweezers and gently places it on the support unit 60 of the operation unit 50. Thereafter, the handle portion 52 is grasped, and the operation portion 50 is lifted to lift the tray 30 and inserted into the container body 4 from the support portion 60 as shown in FIG.

  As shown in FIG. 10, in the middle of inserting the operation unit 50 into the bottom of the container main body 4, the tray 30 is locked and held by a receiving portion 8 formed on a part of the inner wall of the container main body 4. Finally, as shown in FIG. 11, the support part 60 reaches the bottom of the container body 4 as it is and is placed there, and at the same time, the restriction part 90 is positioned so as to cover the entire opening of the tray 30. The restriction unit 90 is set with respect to the tray 30.

After the tray 30 and the operation unit 50 are arranged in the container body 4, a predetermined amount of preservation medium is gently poured into the container body 4. The predetermined amount here may be set as appropriate according to the type of the cultured tissue 100 placed on the placement surface 32, but is preferably at least an amount that allows the cultured tissue 100 to be immersed. The preservation medium may be any liquid as long as it can maintain and preserve the packaged cultured tissue 100 during the transfer period such as transportation, and may be appropriately set according to the cultured tissue 100 to be packaged. Specifically, basal medium, physiological saline, Ringer's solution, phosphate buffer (PBS) and the like can be mentioned.
The operator gently injects the storage medium into the container body 4 through the liquid space 35. When the storage medium is gradually accumulated and reaches the bottom surface of the tray 30, the storage medium enters the tray 30 through the through hole 42 and immerses the cultured tissue 100 placed on the placement surface 32. Furthermore, the air in the container body 4 is removed upward through the space 35, the through hole 42, and the through hole 92 by the injected storage medium. Therefore, air is excluded from the storage space constituted by the tray 30 and the restriction portion 90 and is filled with the storage medium.
In the above description, the storage medium is injected into the container body into which the inner tray is inserted. However, the inner tray may be inserted into the container body into which the storage medium has been injected in advance. In addition, after the cultured tissue 100 is placed on the inner tray, the inner tray is placed on the support portion. However, the cultured tissue 100 may be placed on the inner tray placed on the support portion.

  Thereafter, the operator screws the lid 20 into the container body 4. Thereby, the package 200 in which the tray 30 on which the cultured tissue 100 is placed and the operation unit 50 are accommodated in the form shown in FIGS. 2 and 3 is obtained. By screwing the lid 20 to a predetermined position of the container body 4, the lower surface (ceiling surface) of the lid 20 is brought into contact with the contact portion 46 of the tray 30 and pressed with a predetermined force. It is held by the lid 20 and the receiving part 8. At the same time, the upper end of the handle portion 52 is also brought into contact with and pressed against the lower surface of the lid body 20, whereby the operation portion 50 is held by the bottom portion of the container body 4 and the lid body 20. Further, when the handle portion 52 is pressed, the restricting portion 90 provided integrally with the handle portion 52 is pressed and fixed to the opening of the tray 30, and the entire tray 30 is stably held by the receiving portion 8. Further, when the handle portion 52 of the operation unit 50 is pressed, the tray pressing portion 56 presses the tray 30 against the receiving portion, and the tray 30 is securely held by the receiving portion 8. Thus, the package 200 in which the cultured tissue 100 is held in the tray 30 of the packaging container 2 can be obtained.

(Packaging body)
Since such a package 200 accommodates the operation unit 50 for operating the tray 30 in the packaging container 2, it is easy to take out the cultured tissue 100 after opening. Further, the operation unit 50 is stably held in the packaging container 2 by the container body 4 and the lid 20 so as not to hinder the transportation of the cultured tissue 100. Further, since the tray 30 is held in the packaging container 2 by the contact portion 46 and the receiving portion 8 and is also pressed by the contact portion 54 and the restricting portion 90, the tray 30 is stably held. On the other hand, the operation unit 50 is also stably held in the container body 4.

  In addition, in the accommodation space of the cultured tissue 100 formed in the packaging container 2 by the tray 30 and the regulating portion 90, the circulation of the storage medium is ensured by the through holes 42 and 92 and the state of filling the storage medium is maintained. Is done. In addition, since the accommodation space is configured to be narrow and the movement of the cultured tissue 100 is restricted, even if the packaging container 2 vibrates, the vibration of the cultured tissue 100 can be effectively suppressed and safe transportation can be realized.

(Removal of cultured tissue, etc.)
When using the cultured tissue 100, the package 200 is placed on a clean table, and the operator releases the screwing of the lid 20 and opens the packaging container 2. The operator holds the handle portion 52 of the operation unit 50 and pulls the operation unit 50 and the tray 30 upward. Since the restriction part 90 has the through-hole 92, the storage medium on the restriction part 90 flows down through the through-hole 92, so that the operation part 50 can be easily pulled up. When the handle portion 52 is further lifted, the support portion 60 locks and supports the tray 30 and further pulls up the operation portion 50, so that the operation portion 50 is supported by the support portion 60 while the tray 30 is supported. 4 is taken out. When pulling up from the storage medium, the storage medium in the tray 30 is discharged from the through hole 42 and can be easily pulled up. That is, the storage medium is removed from the tray 30 on which the cultured tissue 100 is placed, and the cultured tissue 100 is placed on the placement surface 32. Further, after removal, the restricting portion 90 is positioned above the tray 30 together with the handle portion 52, and the cultured tissue 100 in the tray 30 is open.

  The tray 30 is taken out by grasping the contact portion 46 of the tray 30 with tweezers or the like from the operation unit 50 taken out, and the tray 30 is placed on the pattern written along the size of the transplant wound 300. Since the placement surface 32 of the tray 30 has transparency, the pattern paper under the placement surface 32 can be viewed from above the placement surface 32. Then, unnecessary portions of the cultured tissue 100 are excised (trimmed) along a pattern that can be visually recognized through the placement surface 32.

  In addition, you may add a washing | cleaning process as needed. In this case, the tray 30 is placed together with the cultured tissue 100 in a dished container such as a petri dish, and the cultured tissue 100 is washed by adding a cleaning solution to the tray 30. Since the tray 30 has the through hole 42, the cleaning liquid accumulates in the container and the tray 30 through the through hole 42. By pulling up the tray 30 from the cleaning liquid, the cleaning liquid drops through the through-hole 32, so that the cleaning can be completed while leaving only the cultured tissue 100 in the tray 30.

  After trimming the cultured tissue 100, the excised unnecessary portion is removed from the tray 30 with tweezers. After the tray 30 on which the cultured tissue 100 trimmed to the transplanted wound 300 is placed is transported to the vicinity of the transplanted wound 300, the tray 30 is tilted as shown in FIG. 12, and the cultured tissue 100 is transplanted from the transfer portion 40. Move to wound 300 and perform transplantation. Since the transfer part 40 has the slope-shaped part 38 which the cultured tissue 100 can follow, the cultured tissue 100 can be reliably transferred toward the target transplantation wound 300. At this time, since the placement surface 32 and the tray 30 are transparent, the cultured tissue 100 can be transplanted while confirming the transplant wound 300 even when the transplant wound 300 is below the tray 30. When the transfer unit 40 is provided with unevenness and surface treatment for controlling the directionality and speed when transferring the cultured tissue 100, the cultured tissue 100 can be transplanted more smoothly or reliably.

  As described above, according to the packaging container using the tray of the present invention, the culture tissue can be smoothly packaged, transported and transplanted. Furthermore, the tray of the present invention can be used not only for transporting cultured tissues but also for storage. Further, the tray of the present invention can easily carry out processing such as packaging, taking out, carrying, trimming and washing of the cultured tissue, and transplantation of the cultured tissue to the transplant site. Furthermore, according to the tray operation unit (operation unit) used for the tray of the present invention, the cultured tissue can be easily packed, taken out and carried, and the tray can be stably held in the packaging container. It is also possible to suppress shaking and vibration of the cultured tissue.

  Further, according to the packaging method of the present invention, by using the packaging container of the present invention, the cultured tissue is packaged in a state suitable for storage, transportation, etc., and an easy-to-use package after opening can be easily obtained. it can. Furthermore, according to the packaging body of the present invention, the cultured tissue can be stored and transported in a good state by using the packaging container of the present invention, and the packaging body has excellent usability after opening.

  The present invention can be implemented in various forms without being limited to the above-described embodiments. For example, in the above embodiment, the tray operation unit 50 is provided separately from the tray 30. However, the present invention is not limited to this, and the tray operation unit 150 is provided to the tray 30 as shown in FIG. It can also be provided integrally. By doing so, the tray 30 can be operated not only as an inner tray of the packaging container 2 but also when it is handled independently from the packaging container 2. The form of the handle portion 152 in this case can take various forms as in the case where the operation portion 50 is provided separately from the tray 30, and can also be a retractable type. That is, in addition to a stick shape or tab shape directed upward from the outer peripheral edge of the tray 30, an arm shape that is bent or curved toward the center side of the container body 4, and an arch shape as shown in FIG. It can be. When the outer periphery of the tray 30 has a portion separated from the inner wall of the container body 4, the handle portion 152 can be easily formed by providing the handle portion 152 so as to be directed upward from the separated portion. Can do. These various types of handle portions 152 can be used as a transport handle portion, and can be used as a transport handle portion and a take-out handle portion. Furthermore, like the handle portion 52 of the embodiment, such a handle portion 152 can be used as the contact portion 154 to the lid body 20 to stably hold the operation portion 150 in the packaging container 2. Note that the contact portion 154 may be separately provided in the operation portion 150. Further, in this modification example, the tray 30 and the operation unit 150 are integrated, so that the handle portion 152 that is in contact with the lower surface of the lid 20 has an abutment portion 146 for fixing the tray 30 to the packaging container 2. Can also function. Further, also in such an operation unit 150, the restriction unit 90 can be provided so as to be openable and closable above the tray 30 or the mounting surface 32. As shown in FIG. Thus, the lower surface of the lid 20 can be used as the restricting portion 90. Furthermore, in the present invention, in order to stably hold the tray 30 in the packaging container, contact portions 46, 54, 154 directed from the tray 30 or the tray operation portions 50, 150 to the lid body 20 are provided. However, an abutting portion directed from the lid 20 toward the tray 30 or the tray operating portions 50 and 150 may be provided. Further, a contact portion directed from the tray operation units 50 and 150 to the tray 30 may be provided.

  Examples of the cultured tissue in which the present invention can be used include various types such as cultured epidermis, cultured skin, cultured cartilage, cultured bone, and cultured corneal epithelium. Suitable for cultured tissue cultured in combination with a three-dimensional scaffold. It is. Moreover, it is suitable for the case where the cultured tissue cultured in a state adhered to the culture container is peeled off and transported.

  The embodiment of the present invention has been described above, but the present invention is not limited to the above description, and can be modified with respect to the same technical idea of the present invention.

  The tray and operation unit of the present invention can be used for transferring cultured tissue at the transplantation site and transporting cultured tissue from the manufacturer to the transplantation destination.

The figure which shows the outline of an example of a packaging container. The figure which shows the AA line cross section of the packaging container shown in FIG. The figure which shows the BB line cross section of the packaging container shown in FIG. The figure which shows the tray for culture tissues. The figure which shows the other example of the transfer part of a tray. The figure which shows the example of the holding structure in the container main body of a tray. The figure which shows the other example of a handle | steering-wheel part. The figure which shows the other example of a control part. It is a figure which shows the state at the time of insertion of the tray to a container main body, (a) is a figure which shows an AA line cross section, (b) is a figure which shows a BB line cross section. It is a figure which shows the state in the middle of insertion of the tray to a container main body, (a) is a figure which shows an AA line cross section, (b) is a figure which shows a BB line cross section. It is a figure which shows the accommodation state of the tray to a container main body, (a) is a figure which shows the AA line cross section, (b) is a figure which shows the BB line cross section. The figure which shows the state which is moving the culture tissue to a transplant wound. The figure which shows the other example of a tray operation part. The figure which shows the other usage type of a tray.

Explanation of symbols

2 packaging container, 4 container body, 4a opening, 8 receiving part, 20 lid, 30 tray, 32 mounting surface, 34 side wall, 36 vertical wall side wall, 38 slope side wall, 40 export part, 42 through hole, 44 Engagement part, 46, 54, 154 Contact part, 48 Engagement part, 50, 150 Tray operation part, 52, 152 Handle part, 54, 154 Contact part, 56 Tray pressing part, 60 Support part, 90 Restriction part , 92 through-hole, 100 cultured tissue, 200 package, 300 transplant wound.

Claims (13)

A culture tissue tray having a placement surface on which the culture tissue can be placed,
A culture tissue tray having the culture tissue transfer portion.   The culture tissue tray according to claim 1, wherein the transfer portion has an opening in the side wall of the tray.   The culture tissue tray according to claim 1 or 2, wherein the export part has a slope-like portion on a side wall of the tray.   The culture tissue tray according to any one of claims 1 to 3, wherein the placement surface has transparency.   The culture tissue tray according to any one of claims 1 to 4, wherein at least one through-hole is provided in any of the placement surface and the side wall of the tray.   The tray for cultured tissues according to any one of claims 1 to 5, wherein the tray has a handle portion for conveyance that can be gripped.   The tray for cultured tissues according to any one of claims 1 to 6, wherein the tray is an inner tray accommodated in a packaging container including a container main body and a lid that can be applied to the container main body.   The culture tissue tray according to claim 7, further comprising an abutting portion that abuts against the lid body when housed in the packaging container.   The culture tissue tray according to claim 7 or 8, wherein the tray has a take-out handle portion that can be gripped when housed in the container body.   The culture tissue tray according to claim 9, wherein the contact portion is at least a part of the take-out handle portion.   The culture tissue tray according to claim 9 or 10, wherein the take-out handle portion is formed so as to be graspable at an upper central portion of the tray.   The culture tissue tray according to any one of claims 9 to 11, wherein the take-out handle portion is formed to be retractable. An operation unit for a culture tissue tray,
The culture tissue tray is the culture tissue tray according to any one of claims 1 to 12,
A support part for supporting the culture tissue tray;
An operation unit comprising a tray operation unit having a handle unit that can be gripped.