JP2007127740A5 - - Google Patents
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- JP2007127740A5 JP2007127740A5 JP2005319100A JP2005319100A JP2007127740A5 JP 2007127740 A5 JP2007127740 A5 JP 2007127740A5 JP 2005319100 A JP2005319100 A JP 2005319100A JP 2005319100 A JP2005319100 A JP 2005319100A JP 2007127740 A5 JP2007127740 A5 JP 2007127740A5
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- laser
- optical path
- unit
- laser light
- observation
- Prior art date
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- 230000003287 optical Effects 0.000 claims 23
- 230000000638 stimulation Effects 0.000 claims 7
- 230000004936 stimulating Effects 0.000 claims 4
- 230000002194 synthesizing Effects 0.000 claims 2
- 230000001678 irradiating Effects 0.000 claims 1
Claims (8)
前記レーザ光を２つの光路に分割する分割手段と、
分割された一方のレーザ光の光路に設けられ、該レーザ光から標本を励起するための所定の波長成分の光を選択する観察用波長選択手段と、
分割された他方のレーザ光の光路に設けられ、該レーザ光から前記標本に刺激を与えるための所定の波長成分の光を選択する刺激用波長選択手段と、
前記観察用波長選択手段からのレーザ光を、対物レンズを介して標本の所定の観察面上で２次元的に走査する観察用走査光学系と、
前記刺激用波長選択手段からのレーザ光を、前記対物レンズを介して前記標本に照射する刺激用光学系と
を具備し、
前記分割手段は、分割比率の異なる複数のビームスプリッタまたはダイクロイックミラーと、この複数のビームスプリッタまたはダイクロイックミラーを選択的に光路へ配置する切り替え手段を備える走査型レーザ顕微鏡装置。 A light source unit for generating laser light having a plurality of different wavelengths;
Splitting means for splitting the laser light into two optical paths;
An observation wavelength selection unit that is provided in an optical path of one of the divided laser beams and that selects light of a predetermined wavelength component for exciting the sample from the laser beam;
A wavelength selection unit for stimulation that is provided in the optical path of the other divided laser beam and selects light of a predetermined wavelength component for stimulating the specimen from the laser beam;
An observation scanning optical system that two-dimensionally scans a laser beam from the observation wavelength selection unit on a predetermined observation surface of the sample via an objective lens;
A stimulating optical system for irradiating the sample with laser light from the stimulating wavelength selecting means via the objective lens , and
The splitting means is a scanning laser microscope apparatus comprising a plurality of beam splitters or dichroic mirrors having different split ratios, and a switching means for selectively arranging the plurality of beam splitters or dichroic mirrors in the optical path .
前記分割手段と前記刺激用光学系との間の光路に刺激用調光手段が設けられている請求項１に記載の走査型レーザ顕微鏡装置。 Observation dimming means is provided in the optical path between the dividing means and the observation scanning optical system,
The scanning laser microscope apparatus according to claim 1, wherein stimulation light adjustment means is provided in an optical path between the dividing means and the stimulation optical system.
互いに異なる波長のレーザ光を発する複数のレーザ光源と、
前記レーザ光源から発せられた各レーザ光の光路を合成する光路合成手段と
を備える請求項１から請求項３のいずれかに記載の走査型レーザ顕微鏡装置。 The light source unit is
A plurality of laser light sources that emit laser beams of different wavelengths;
The scanning laser microscope apparatus according to any one of claims 1 to 3 , further comprising: an optical path combining unit that combines optical paths of the laser beams emitted from the laser light source.
前記分割手段と前記観察用波長選択手段との間に設けられ、前記分割手段からのレーザ光の光路と前記観察用レーザ光源からのレーザ光の光路とを合成する光路合成手段と
を具備する請求項１から請求項４のいずれかに記載の走査型レーザ顕微鏡装置。 At least one observation laser light source that emits laser light of a wavelength that is used only to excite the specimen;
An optical path synthesizing unit that is provided between the dividing unit and the observation wavelength selecting unit and synthesizes the optical path of the laser beam from the dividing unit and the optical path of the laser beam from the observation laser light source. The scanning laser microscope apparatus according to any one of claims 1 to 4 .
前記分割手段と前記刺激用波長選択手段との間に設けられ、前記分割手段からのレーザ光の光路と前記刺激用レーザ光源からのレーザ光の光路とを合成する光路合成手段と
を具備する請求項１から請求項４のいずれかに記載の走査型レーザ顕微鏡装置。 At least one stimulation laser light source that emits laser light of a wavelength that is used only to stimulate the specimen;
An optical path synthesizing unit that is provided between the dividing unit and the stimulation wavelength selecting unit and synthesizes the optical path of the laser beam from the dividing unit and the optical path of the laser beam from the stimulation laser light source. The scanning laser microscope apparatus according to any one of claims 1 to 4 .
前記レーザ光を２つの光路に分割する分割手段と、
ｇｆ分割された一方のレーザ光の光路に設けられ、該レーザ光から標本を励起するための所定の波長成分の光を選択する観察用波長選択手段と、
分割された他方のレーザ光の光路に設けられ、該レーザ光から前記標本に刺激を与えるための所定の波長成分の光を選択する刺激用波長選択手段と
を具備し、
前記分割手段は、分割比率の異なる複数のビームスプリッタまたはダイクロイックミラーと、この複数のビームスプリッタまたはダイクロイックミラーを選択的に光路へ配置する切り替え手段を備える顕微鏡用照明装置。 A light source unit for generating laser light having a plurality of different wavelengths;
Splitting means for splitting the laser light into two optical paths;
an observation wavelength selection unit that is provided in the optical path of one of the laser beams divided by gf, and that selects light having a predetermined wavelength component for exciting the sample from the laser beam;
Provided in the optical path of the other divided laser light, and comprises a wavelength selection means for stimulation for selecting light of a predetermined wavelength component for stimulating the specimen from the laser light ,
The microscope illuminating apparatus comprising: a plurality of beam splitters or dichroic mirrors having different division ratios; and a switching unit that selectively arranges the plurality of beam splitters or dichroic mirrors in an optical path .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005319100A JP2007127740A (en) | 2005-11-02 | 2005-11-02 | Scan type laser microscope apparatus and microscope illumination apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005319100A JP2007127740A (en) | 2005-11-02 | 2005-11-02 | Scan type laser microscope apparatus and microscope illumination apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2007127740A JP2007127740A (en) | 2007-05-24 |
| JP2007127740A5 true JP2007127740A5 (en) | 2008-12-18 |
Family
ID=38150457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005319100A Pending JP2007127740A (en) | 2005-11-02 | 2005-11-02 | Scan type laser microscope apparatus and microscope illumination apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2007127740A (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009139479A (en) * | 2007-12-04 | 2009-06-25 | Hitachi Kokusai Electric Inc | Image processing apparatus |
| DE102010047353A1 (en) | 2010-10-01 | 2012-04-05 | Carl Zeiss Microimaging Gmbh | Laser Scanning Microscope with switchable mode of operation |
| JP5907998B2 (en) * | 2011-03-01 | 2016-04-26 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | Variable orientation lighting pattern rotator |
| JP6636756B2 (en) * | 2015-09-10 | 2020-01-29 | 株式会社東芝 | Optical device and processing device |
| DE102016102286A1 (en) * | 2016-02-10 | 2017-08-10 | Carl Zeiss Microscopy Gmbh | Apparatus and method for multispot scanning microscopy |
| JP6803196B2 (en) * | 2016-10-27 | 2020-12-23 | オリンパス株式会社 | Microscope device |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001117013A (en) * | 1999-10-20 | 2001-04-27 | Olympus Optical Co Ltd | Confocal laser microscope |
| JP4632634B2 (en) * | 2002-03-27 | 2011-02-16 | オリンパス株式会社 | Confocal microscope apparatus and observation method using confocal microscope apparatus |
| JP4242617B2 (en) * | 2002-08-28 | 2009-03-25 | オリンパス株式会社 | Scanning laser microscope system |
| JP4422425B2 (en) * | 2003-04-07 | 2010-02-24 | オリンパス株式会社 | Microscope system |
| JP2005181891A (en) * | 2003-12-22 | 2005-07-07 | Olympus Corp | Laser microscope |
-
2005
- 2005-11-02 JP JP2005319100A patent/JP2007127740A/en active Pending
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