JP2007093443A - Plate position aligning device, dispensing apparatus, and biosensor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a plate position aligning device capable of holding a plate at an appropriate access position regardless of the size of the plate, and to provide a biosensor and a dispensing apparatus equipped with the plate position aligning device. <P>SOLUTION: A plate holding member 80 for holding an analyte solution plate 84 includes: a mount plate 81 for mounting the analyte solution plate 84; a first adjusting section 82A; a first biasing section 82B; a second adjusting section 83A; a second biasing section 83B; and a projection section 80H for positioning a display plate in height. The first adjusting section 82A is composed of a first receiving section 82U, a first thread section 82N and a first fixing section 82K, wherein the first thread section 82N is rotated along a thread groove, whereby the first receiving section 82U is moved in Y-direction. The first biasing section 82B comes into contact with the analyte solution plate 84, thereby biasing the analyte solution plate 84 to the first adjusting section 82A. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a plate position adjusting device that adjusts the position of a plate accessed by a pipette, a dispensing device including the plate position adjusting device, and a biosensor.

  An automatic dispensing device is known as a device for supplying a sample for measuring an interaction between biochemical substances such as protein and DNA, screening a drug, and the like. In an automatic dispensing device, a plate in which a large number of recesses are formed is usually set in a matrix, the sample in the recesses is sucked by a pipette, the pipette moves to another position, and the sucked sample is discharged. A dispensing operation is performed (see Patent Document 1).

  In this dispensing operation, in order to allow the pipette to access the concave portion of the plate, it is necessary to arrange the plate so that the concave portion of the plate coincides with the access position of the pipette.

Usually, the recesses of this type of plate and the interval between the recesses are the same size. However, there are various sizes of distances between the recessed portion and the outer peripheral end, and it is necessary to prepare a plurality of types of holding members having different holding positions depending on the type of plate.
Japanese Patent Laid-Open No. 11-223636

  The present invention has been made in consideration of the above-mentioned facts, and a plate position adjusting device capable of holding a plate in an appropriate access position regardless of the size of the plate, and a dispensing system equipped with this plate position adjusting device. An object is to provide a device and a biosensor.

  In order to solve the above-described problem, a plate position adjusting device according to a first aspect of the present invention includes a plate having a portion to be accessed that has a predetermined relationship with an access position accessed by a pipette and is accessed by the pipette. A holding member to be held; an access position indicating member that indicates the access position; and an arrangement position display member that indicates an arrangement position of the accessed portion with respect to the holding member; and the access portion is provided at the arrangement position. And adjusting means for moving and adjusting the holding position of the plate on the holding member.

  The holding member of the plate position adjusting device has a predetermined relationship with the access position accessed by the pipette. The holding member holds a plate having an accessed portion that receives access from the pipette. The arrangement position display member indicates the access position of the pipette and the arrangement position of the accessed portion with respect to the holding member. That is, when the accessed part is arranged at the indicated arrangement position, the pipette is correctly accessed to the accessed part. Therefore, in the present invention, the holding position of the plate with the holding member is moved and adjusted so that the accessed portion is arranged at the arrangement position by the adjusting means. The adjustment here is performed by moving the relative position of the plate and the holding member.

  According to the said structure, a plate can be hold | maintained to an appropriate access position irrespective of the size of a plate by adjusting the arrangement position of a plate with an adjustment means.

  The adjusting means of the plate position adjusting device according to the first aspect contacts the first side surface of the plate and moves in a direction orthogonal to the first side surface to move the position of the first side surface. A member, a first biasing member that biases the plate toward the first adjustment member, and a second side surface that is not parallel to the first side surface and moves in a direction perpendicular to the second side surface. A second adjustment member that moves the position of the second side surface and a second urging member that urges the plate toward the second adjustment member may be included.

  In the adjusting means having the above configuration, the first adjusting member abuts on the first side surface of the plate to define the position of the first side surface. The first adjustment member is movable in a direction orthogonal to the first side surface. Since the plate is urged toward the first adjustment member by the first urging member, the position of the first side surface can be moved by moving the first adjustment member. On the other hand, the second adjusting member also contacts the second side surface of the plate to define the position of the second side surface. The second adjustment member is movable in a direction orthogonal to the second side surface. Since the plate is urged toward the second adjustment member by the second urging member, the position of the second side surface can be moved by moving the second adjustment member.

  Further, the arrangement position display member of the plate position adjusting device according to the first aspect is displayed on the upper surface of the frame member and the frame member formed on the upper side of the plate and having an opening exposing the accessed portion. A display unit indicating the arrangement position may be provided.

  Thus, by covering the frame member on the plate and forming the display portion on the upper surface of the frame member, the display portion and the accessed portion can be easily seen and alignment can be facilitated.

  A dispensing apparatus according to a second aspect of the present invention includes a plate position adjusting device according to any one of claims 1 to 3 and an access target of a plate held by a holding member of the plate position adjusting device. A dispensing head to which a pipette that accesses the unit is attached, and a conveying unit that conveys the plate held by the holding member.

  The dispensing device configured as described above can hold the plate at an appropriate access position regardless of the size of the plate by adjusting the position of the plate with the adjusting means, thus making it easy to control the dispensing head. In addition, the holding plate having a fixed shape can be easily conveyed by the conveying means.

  A biosensor according to a third aspect of the present invention is a biosensor that measures the reaction state of a sample using attenuation of total reflection of light, and includes a flow path having a supply port and a discharge port, 5. A measurement unit having a sensor surface on which a ligand is fixed on an exposed flat metal film, and the dispensing apparatus according to claim 4, wherein the sample is fed to the sensor surface of the measurement unit, and the sensor. An optical member that emits light toward the metal film of the chip and a light receiving member that receives the light reflected by the metal film are included.

  According to the biosensor configured as described above, the plate can be held at an appropriate access position regardless of the size of the plate by adjusting the position of the plate by the adjusting means, so that the dispensing head can be easily controlled. In addition, the holding plate having a fixed shape can be easily conveyed by the conveying means.

  Since the present invention is configured as described above, the plate can be held at an appropriate access position regardless of the size of the plate.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  The biosensor 10 of the present invention is a so-called surface plasmon sensor that measures the interaction between the ligand D and the analyte A by utilizing surface plasmon resonance generated on the surface of a metal film.

  As shown in FIG. 1, the biosensor 10 includes a tray holding unit 12, a transport unit 14, a container mounting table 16, a liquid suction / discharge unit 20, an optical measurement unit 54, a transport arm 70, and a control unit 60.

  The tray holding unit 12 includes a mounting table 12A and a belt 12B. The mounting table 12A is attached to a belt 12B spanned in the arrow Y direction, and is movable in the arrow Y direction by the rotation of the belt 12B. A tray T is placed on the mounting table 12A. A sensor stick 40 is stored in the tray T. The sensor stick 40 is a chip to which the ligand D is fixed, and details will be described later. Under the mounting table 12A, a push-up mechanism 12D that pushes up the sensor stick 40 to a position of a stick holding member 14C described later is disposed.

  As shown in FIGS. 2 and 3, the sensor stick 40 includes a dielectric block 42, a flow path member 44, a holding member 46, an adhesive member 48, and an evaporation preventing member 49.

  The dielectric block 42 is made of transparent resin or the like that is transparent to the light beam, and has a prism portion 42A having a trapezoidal cross section, and the prism portion 42A integrally with both ends of the prism portion 42A. The formed held portion 42B is provided. A metal film 50 is formed on the upper surface on the wider side of the two parallel surfaces of the prism portion 42A as shown in FIG. On this metal film 50, the ligand D to be analyzed by the biosensor 10 is fixed. The dielectric block 42 functions as a so-called prism. When the measurement is performed by the biosensor 10, a light beam is incident from one of two opposing non-parallel sides of the prism portion 42A, and a metal film is formed from the other. The light beam totally reflected at the interface with 50 is emitted.

  As shown in FIG. 4, a linker layer 50 </ b> A is formed on the surface of the metal film 50. The linker layer 50 </ b> A is a layer for immobilizing the ligand D on the metal film 50. On the linker layer 50A, the measurement region (E1) in which the ligand D is fixed and the reaction between the analyte A and the ligand D occurs, and the reference signal for signal measurement in the measurement region E1 is obtained without the ligand D being fixed. And a reference region (E2) for forming the same. The reference region E2 is formed when the linker layer 50A described above is formed. As a formation method, for example, the linker layer 50A is subjected to a surface treatment to deactivate a binding group that binds to the ligand D. Thereby, half of the linker layer 50A becomes the measurement region E1, and the other half becomes the reference region E2. Thus, in order to deactivate a bonding group, the ethanolamine-hydrochloride used for the above-mentioned blocking can be used. As another configuration method of the reference region E2, for example, if alkyl thiol is arranged in the reference region E2 instead of carboxymethyl dextran, an alkyl group can be arranged on the surface. Since the ligand cannot be bound by the coupling method, it can be used as the reference region E2.

  As shown in FIG. 5, the ligand D is fixed to a portion of the linker layer 50 </ b> A exposed to the liquid channel 45 other than the reference region E <b> 2. The ligand D is not fixed in the reference region E2. Light beams L2 and L1 are incident on the reference region E2 and the measurement region E1 located on the upstream side of the reference region E2, respectively. The reference area E2 is an area provided for correcting data obtained from the measurement area E1 where the ligand D is fixed.

  On both side surfaces of the prism portion 42A, engaging convex portions 42C that are engaged with the holding member 52 along the upper edge are virtual surfaces perpendicular to the upper surface of the prism portion 42A along the lower edge. Vertical protrusions 42D configured on the extension are formed at seven locations, respectively. Further, an engagement groove 42E is formed in the center portion along the longitudinal direction of the lower surface of the dielectric block 42.

  The flow path member 44 has a rectangular parallelepiped shape slightly narrower than the dielectric block 42, and is arranged side by side on the metal film 50 of the dielectric block 42 as shown in FIG. 3. A channel groove 44 </ b> A is formed on the lower surface of each channel member 44, communicated with the supply port 45 </ b> A and the discharge port 45 </ b> B formed on the upper surface, and the liquid channel 45 between the metal film 50. Composed. Accordingly, six independent liquid channels 45 are formed in one sensor stick 40. On the side wall of the flow path member 44, a convex portion 44 </ b> B is formed that is press-fitted into a concave portion (not shown) inside the holding member 46 to ensure adhesion with the holding member 46.

  In addition, since it is assumed that the liquid flow path 45 is supplied with a liquid containing protein, in order to prevent the protein from adhering to the flow path member 44, the material of the flow path member 44 is a non-protein. It is preferable not to have specific adsorptivity.

  The holding member 46 is long and includes an upper plate 46A and two side plates 46B. The side plate 46B is formed with an engagement hole 46C that is engaged with the engagement convex portion 42C of the dielectric block 42. The holding member 46 is attached to the dielectric block 42 with the engagement holes 46 </ b> C and the engagement protrusions 42 </ b> C engaged with the six flow path members 44 interposed therebetween. Thereby, the flow path member 44 is attached to the dielectric block 42. In the upper surface plate 46A, a tapered pipette insertion hole 46D that narrows toward the flow path member 44 is formed at a position facing the supply port 45A and the discharge port 45B of the flow path member 44. A positioning boss 46E is formed between the adjacent pipette insertion hole 46D and the pipette insertion hole 46D.

  An evaporation preventing member 49 is bonded to the upper surface of the holding member 46 via an adhesive member 48. A pipette insertion hole 48D is formed at a position facing the pipette insertion hole 46D of the adhesive member 48, and a positioning hole 48E is formed at a position facing the boss 46E. Further, a slit 49D, which is a cross-shaped cut, is formed at a position facing the pipette insertion hole 46D of the evaporation preventing member 49, and a positioning hole 49E is formed at a position facing the boss 46E. By inserting the boss 46E into the holes 48E and 49E and bonding the evaporation preventing member 49 to the upper surface of the holding member 52, the slit 49D of the evaporation preventing member 49 and the supply port 45A and the discharge port 45B of the flow path member 44 are formed. Configured to face each other. When the pipette tip CP is not inserted, the slit 49D covers the supply port 45A, and evaporation of the liquid supplied to the liquid channel 45 is prevented.

  As shown in FIG. 1, the transport unit 14 of the biosensor 10 includes an upper guide rail 14A, a lower guide rail 14B, and a stick holding member 14C. The upper guide rail 14 </ b> A and the lower guide rail 14 </ b> B are horizontally disposed in the arrow X direction perpendicular to the arrow Y direction, above the tray holding unit 12 and the optical measurement unit 54. A stick holding member 14C is attached to the upper guide rail 14A. The stick holding member 14C can hold the held parts 42B at both ends of the sensor stick 40 and can move along the upper guide rail 14A. The engagement groove 42E of the sensor stick 40 held by the stick holding member 14C and the lower guide rail 14B are engaged, and the stick holding member 14C moves in the arrow X direction, so that the sensor stick 40 is placed on the optical measurement unit 54. To the measurement unit 56. The measuring unit 56 is provided with a pressing member 58 that presses the sensor stick 40 during measurement. The pressing member 58 is movable in the Z direction by a driving mechanism (not shown), and presses the sensor stick 40 disposed in the measurement unit 56 from above.

  The liquid suction / discharge section 20 includes a head 24. The head 24 is movable in the arrow Y direction along a conveyance rail (not shown). The head 24 is also movable in the vertical direction (arrow Z direction) by a drive mechanism (not shown) inside the head 24. As shown in FIG. 6, the head 24 includes a pair of pipette portions 24A and 24B. Pipette tips CP are attached to the tip portions of the pipette portions 24A and 24B, and the length in the Z direction can be individually adjusted.

  As shown in FIG. 1, the transfer arm 70 includes two sandwiching portions 70 </ b> A arranged to face each other, and the analyte solution plate 84 and the chip stocker 18 can be sandwiched between the sandwiching portions 70 </ b> A. The transfer arm 70 is connected to a drive mechanism (not shown) and is movable in the X, Y, and Z directions.

  On the container mounting table 16, an analyte solution plate 84, a chip stocker 18, and a buffer solution stock container 19 are mounted. The buffer solution stock container 19 includes containers 19A to 19E, and various buffer solutions are stocked. In the containers 19A to 19E, an opening K into which a pipette tip CP described later can be inserted is formed.

  A plurality of chip stockers 18 are prepared in the chip stock unit 18A. The chip stocker 18 is taken out from the chip stock unit 18A by the transfer arm 70 and arranged at a predetermined position on the container mounting table 16. A replacement pipette tip CP is stocked on the tip stocker 18.

  As shown in FIG. 7, the analyte solution plate 84 has a rectangular plate shape, and is partitioned into a matrix to form a plurality of recesses 84A. Various analyte solutions are stocked in the recess 84A. One side surface along the longitudinal direction of the analyte solution plate 84 is hereinafter referred to as a “first side surface 84B”, and one side surface along the short direction of the analyte solution plate 84 is hereinafter referred to as a “second side surface 84C”. Further, the line of the recess 84A that is configured closest to the first side surface 84B is referred to as “first recess line 84D”, and the line of the recess 84A that is configured closest to the second side surface 84C is “second recess line 84E”. And

  The plate holding member 80 that holds the analyte solution plate 84 includes a rectangular plate-like placement plate 81 on which the analyte solution plate 84 is placed, a first adjustment portion 82A, a first biasing portion 82B, and a second adjustment portion 83A. The second urging portion 83B and the display plate height determining convex portion 80H are provided.

  82 A of 1st adjustment parts are attached to two places along the elongate edge on the mounting plate 81, and include the 1st receiving part 82U, the 1st screw part 82N, and the 1st fixing | fixed part 82K. It consists of The first fixing portion 82K is fixed on the mounting plate 81 and has a screw groove that is screwed with the first screw portion 82N. The first screw portion 82B is screwed into the screw groove of the first fixing portion 82K, and can move in the Y direction orthogonal to the first side surface 84B by rotating along the screw groove. The first receiving portion 82U is in contact with the first side surface 84B of the analyte solution plate 84 and defines the position of the first side surface 84B.

  The first urging portion 82B is provided at the center in the longitudinal direction of the other end side portion of the mounting plate 81 facing the first adjustment portion 82A. The first urging portion 82B is configured to include a coil spring, abuts on the analyte solution plate 84, and urges the analyte solution plate 84 toward the first adjustment portion 82A.

  The second adjustment portion 83A is attached to the center of the short end side portion on the mounting plate 81, and includes the second receiving portion 83U, the second screw portion 83N, and the second fixing portion 83K. ing. The second fixing portion 83K is fixed on the mounting plate 81 and has a screw groove that is screwed with the second screw portion 83N. The second screw portion 83B is screwed into the screw groove of the second fixing portion 83K, and can move in the X direction orthogonal to the second side surface 84B by rotating along the screw groove. The second receiving portion 83U is in contact with the second side surface 84C of the analyte solution plate 84 and defines the position of the second side surface 84C.

  The second urging portion 83B is provided on the other end side of the mounting plate 81 so as to face the second adjusting portion 83A. The second urging portion 83B is also configured to include a coil spring, and abuts on the analyte solution plate 84 to urge the analyte solution plate 84 toward the second adjustment portion 83A.

  The display plate height determining convex portion 80H is erected at four corners of the mounting plate 81, and includes a lower portion 80D having a larger diameter than an insertion hole 85B described later and an upper portion 80U having a small diameter. The height of the lower portion 80 </ b> D is set such that the lower surface of the arrangement position display member 85 to be attached comes into contact with the analyte solution plate 84.

  The arrangement position display member 85 has a frame shape in which an opening 85A in which the concave portion 84A portion of the analyte solution plate 84 is exposed is formed at the center of the rectangular plate member. On the arrangement position display member 85, an extended portion of the access position of the pipette tip CP closest to the first adjustment portion 82A is displayed in a line (hereinafter, this display is referred to as “first access line 85C”). In addition, on the arrangement position display member 85, an extended portion of the access position of the pipette chip CP closest to the second adjustment portion 83A is displayed in a line shape (this display is hereinafter referred to as “second access line 85D”). Further, insertion holes 85B into which the upper portions 80U of the display plate height determining convex portions 80H are inserted are formed at the four corners of the arrangement position display member 85.

  The analyte solution plate 84 is held on the plate holding member 80, and the arrangement position display member 85 is attached, and after the alignment is completed (see FIG. 8), the analyte solution plate 84 is stocked in the analyte solution plate stock section 17. ing. The analyte solution plate 84 in this state is taken out from the analyte solution plate stock unit 17 by the transfer arm 70 and placed at a predetermined position on the container mounting table 16. The predetermined position here is determined in advance in relation to the access position of the pipette tip CP attached to the pipette portions 24A and 24B of the head 24.

  Alternatively, after the alignment, the arrangement position display member 85 may be removed and stocked in the analyte solution plate stock unit 17 and may be arranged as it is at a predetermined position on the container mounting table 16.

  The analyte solution plate 84 is attached to the plate holding member 80 having the above-described configuration as follows.

  First, the analyte solution plate 84 is placed on the placement plate 81, and the placement position display member 85 is placed thereon, and the upper portion 80U of the display plate height determining convex portion 80H is inserted into the insertion hole 85B (FIG. 9). (See (A)). The user rotates the first screw portion 82N and moves the first receiving portion 82U so that the first access line 85C and the first recess line 84D are in a straight line. Further, the second receiving portion 83U is moved by rotating the second screw portion 83N so that the second access line 85D and the second recessed portion line 84E are in a straight line (see FIG. 9B). In this manner, the analyte solution plate 84 can be positioned on the plate holding member 80.

  As shown in FIG. 10, the optical measurement unit 54 includes a light source 54A, a first optical system 54B, a second optical system 54C, a light receiving unit 54D, and a signal processing unit 54E. A divergent light beam L is emitted from the light source 54A. The light beam L becomes two light beams L1 and L2 via the first optical system 54B, and is incident on the measurement region E1 and the reference region E2 of the dielectric block 42 arranged in the measurement unit 56. In the measurement region E1 and the reference region E2, the light beams L1 and L2 include various incident angle components with respect to the interface between the metal film 50 and the dielectric block 42 and are incident at an angle greater than the total reflection angle. The light beams L 1 and L 2 are totally reflected at the interface between the dielectric block 42 and the metal film 50. The totally reflected light beams L1 and L2 are also reflected with various reflection angle components. The totally reflected light beams L1 and L2 are received by the light receiving unit 54D through the second optical system 54C, are photoelectrically converted, and a light detection signal is output to the signal processing unit 54E. In the signal processing unit 54E, predetermined processing is performed based on the input photodetection signal, and total reflection attenuation angle data (hereinafter referred to as “total reflection attenuation angle data”) of the measurement region E1 and the reference region E2 is obtained. . The total reflection attenuation angle data is output to the control unit 60.

  The control unit 60 has a function of controlling the entire biosensor 10, and is connected to a light source 54A, a signal processing unit 54E, and a drive system (not shown) of the biosensor 10 as shown in FIG. As shown in FIG. 11, the control unit 60 includes a CPU 60A, a ROM 60B, a RAM 60C, a memory 60D, and an interface I / F 60E that are connected to each other via a bus B, and a display unit 62 that displays various types of information. These are connected to an input unit 64 for inputting various instructions and information.

  Various programs for controlling the biosensor 10 and various data are recorded in the memory 60D.

  Next, measurement with the biosensor 10 will be described.

  On the mounting table 12A of the biosensor 10, a tray containing the sensor stick 40 in which the ligand D is fixed and the liquid channel 45 is filled with the storage solution C is set. The analyte solution plate 84 is set with a predetermined analyte solution.

  First, one sensor stick 40 is pushed up to the position of the stick holding member 14C by the push-up mechanism 12D, and is held by the stick holding member 14C. The stick holding member 14 </ b> C moves along the lower guide rail 14 </ b> B while holding the sensor stick 40, and conveys the sensor stick 40 to the measuring unit 56. The sensor stick 40 conveyed to the measurement unit 56 is positioned at a predetermined measurement position and is pressed and fixed from above by a pressing member 58.

  When an instruction to start measurement is input from the input unit 64, the control unit 60 executes the measurement process shown in FIG.

  First, in step S12, an output instruction signal for the light beam L is output to the light source 54A. Thereby, the light beam L is emitted from the light source 54A. The emitted light beam L is converted into two light beams L1 and L2 by the first optical system 54B, and is incident on the measurement region E1 and the reference region E2 of the liquid channel 45, respectively. In step S14, an operation instruction signal is output to the light receiving unit 54D and the signal processing unit 54E. Thus, the light beams L1 and L2 that are totally reflected by the measurement region E1 and the reference region E2 and pass through the second optical system 54C are received by the light receiving unit 54D, and the received light is received for each of the measurement region E1 and the reference region E2. The photoelectric conversion is performed and a light detection signal is output to the signal processing unit 54E. In the signal processing unit 54E, predetermined processing is applied to the light detection signal, total reflection attenuation angle data is generated, and output to the control unit 60.

  In step S16, the controller 60 determines whether or not a predetermined time has elapsed. After the predetermined time has elapsed, the controller 60 stores the input total reflection attenuation angle data in the memory 60D in step S18. In step S20, the total reflection attenuation angle data obtained from the photodetection signal from the measurement region E1 is corrected with the total reflection attenuation angle data obtained from the photodetection signal from the reference region E2, and the ligand D and the analyte are corrected. Binding state data indicating the binding state with the analyte A in the solution YA is generated. In step S22, the combined state data is output to the display unit 62. Thereby, the coupling state data for every predetermined time is stored in the memory 60D and displayed on the display unit 62. To the display unit 62, the combined state data for each time is graphed and output. This measurement process is continued until a measurement process end signal is received.

  On the other hand, when an instruction to start supplying analyte is input from the input unit 64, the control unit 60 executes the analyte supply process shown in FIG.

  First, in step S30, the analyte solution YA is obtained by sucking the analyte solution YA into the pipette tip CPA. That is, the head 24 is moved to the upper part of the analyte solution plate 84 in which the analyte solution YA is set, the pipette part 24A is lowered, and only the tip of the pipette tip CPA attached to the pipette part 24A is moved. Insert into the cell where the analyte solution YA is stored. Aspirate the analyte solution YA into the pipette tip CPA.

  At this time, since the analyte solution plate 84 is accurately positioned on the plate holding member 80, the pipette tip CP can be accurately accessed to the predetermined recess 84A.

  Next, in step S31, the head 24 is moved onto the sensor stick 40, and in step S32, the pipette tip CPA is inserted into the supply port 45A, and the pipette tip CPB is inserted into the discharge port 45B.

  In step S33, the analyte solution YA is discharged from the pipette tip CPA, and the buffer solution filled in the liquid channel 45 is sucked into the pipette tip CPB. Thereby, the buffer solution in the liquid channel 45 is replaced with the analyte solution YA.

  In step S34, the process waits until a predetermined time elapses. The predetermined time here is set to a time sufficient for the reaction between the analyte A and the ligand D. After a predetermined time has elapsed, this process is terminated.

  According to the present embodiment, since the position of the analyte solution plate 84 can be adjusted on the plate holding member 80, the same plate holding member 80 can be used corresponding to the analyte solution plates 84 of various sizes. . Therefore, it is not necessary to prepare a plurality of types of plate holding members, and the cost can be reduced.

  In this embodiment, positioning is performed by placing the arrangement position display member 85 on the analyte solution plate 84. However, the arrangement position is displayed directly on the plate holding member 80 as shown in FIG. The ribs 80J and 80K corresponding to the positions of the first access line 85C and the second access line 85D may be provided, and positioning may be performed by aligning the ribs with the first concave line 84D and the second concave line 84E. Good.

  In the present embodiment, the surface plasmon sensor is described as an example of the biosensor, but the biosensor is not limited to the surface plasmon sensor. In addition, for example, quartz crystal microbalance (QCM) measurement technology, optical measurement technology using a functionalized surface from colloidal gold particles to ultrafine particles, and the like. Can be applied.

  In addition, a leaky mode detector can be used as another biosensor using total reflection attenuation. The leakage mode sensor is composed of a dielectric, and a thin film composed of a clad layer and an optical waveguide layer that are sequentially layered thereon. One surface of the thin film serves as a sensor surface, and the other surface receives light. It becomes a surface. When light is incident on the light incident surface so as to satisfy the total reflection condition, a part of the light is transmitted through the cladding layer and taken into the optical waveguide layer. In this optical waveguide layer, when the waveguide mode is excited, the reflected light at the light incident surface is greatly attenuated. The incident angle at which the waveguide mode is excited varies according to the refractive index of the medium on the sensor surface, similarly to the surface plasmon resonance angle. By detecting the attenuation of the reflected light, the reaction on the sensor surface can be measured.

It is a whole perspective view of the biosensor of this embodiment. It is a perspective view of the sensor stick of this embodiment. It is a disassembled perspective view of the sensor stick of this embodiment. It is sectional drawing of the 1 liquid flow path part of the sensor stick of this embodiment. It is a figure which shows the state in which the light beam is injecting into the measurement area | region and reference area | region of the sensor stick of this embodiment. It is a schematic block diagram of the liquid suction / discharge part of this embodiment. It is a disassembled perspective view which shows the relationship between the plate holding member of this embodiment, an analyte solution plate, and an arrangement position display member. It is a perspective view which shows the state by which the analyte solution plate and the arrangement position display member were attached to the plate holding member of this embodiment. (A) is an analyte solution plate before alignment, and (B) is a top view showing a state where the analyte solution plate after alignment is attached to a plate holding member. Book It is the schematic of the optical measurement part vicinity of the biosensor of this embodiment. It is a schematic block diagram of the control part of this embodiment, and its periphery. It is a flowchart of the measurement process of this embodiment. It is a flowchart of the analyte supply process of this embodiment. It is a perspective view which shows the modification of the plate holding member of this embodiment.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Biosensor 16 Container mounting stand 17 Analyte solution plate stock part 24A Pipette part 24B Pipette part 24 Head 40 Sensor stick 50 Metal film 54 Optical measurement part 54A Light source 54D Light receiving part 54E Signal processing part 70 Transfer arm 80 Plate holding member 80J Rib 80K rib 81 mounting plate 82A first adjusting portion 82B first biasing portion 83A second adjusting portion 83B second biasing portion 84 analyte solution plate 84A recess 84B first side surface 84C second side surface 85C first access line 85D first 2 Access line 85 Arrangement position display member

Claims (5)

A holding member for holding a plate having a portion to be accessed, which has a predetermined relationship with an access position accessed by a pipette and is accessed by the pipette;
An arrangement position display member indicating the access position and indicating an arrangement position of the accessed portion relative to the holding member
Adjusting means provided on the holding member and moving and adjusting the holding position of the plate at the holding member so that the accessed portion is arranged at the arrangement position;
A plate position adjusting device.   The adjusting means contacts a first side surface of the plate and moves in a direction orthogonal to the first side surface to move the position of the first side surface; and the plate moves the first adjusting member. A first urging member that urges toward the second side, and a second side that abuts against a second side surface that is not parallel to the first side surface and moves in a direction orthogonal to the second side surface to move the position of the second side surface. The plate position adjusting device according to claim 1, comprising an adjustment member and a second urging member that urges the plate toward the second adjustment member.   The arrangement position display member includes a frame member that covers the upper side of the plate and has an opening that exposes the accessed portion, and a display unit that is displayed on the upper surface of the frame member and indicates the arrangement position. The plate position adjusting device according to claim 1, wherein the plate position adjusting device is provided. The plate position adjusting device according to any one of claims 1 to 3,
A pipetting head with a pipette attached to access the accessed portion of the plate held by the holding member of the plate position adjusting device;
Conveying means for conveying the plate held by the holding member;
Dispensing device equipped with. A biosensor that measures the reaction state of a sample using attenuation of total reflection of light,
A measurement unit having a flow path having a supply port and a discharge port, and a sensor surface having a ligand fixed on a flat metal film exposed in the flow path,
The dispensing apparatus according to claim 4, wherein the sample is fed to the sensor surface of the measurement unit;
An optical member that emits light toward the metal film of the sensor chip;
A light receiving member that receives the light reflected by the metal film;
A biosensor with

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