JP2007061060A - Vegetable beverage containing fermented milk and method for producing the same - Google Patents

Vegetable beverage containing fermented milk and method for producing the same Download PDF

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JP2007061060A
JP2007061060A JP2005254308A JP2005254308A JP2007061060A JP 2007061060 A JP2007061060 A JP 2007061060A JP 2005254308 A JP2005254308 A JP 2005254308A JP 2005254308 A JP2005254308 A JP 2005254308A JP 2007061060 A JP2007061060 A JP 2007061060A
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fermented milk
tomato
vegetable
juice
milk
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Koichi Yoshimura
公一 吉村
Mutsumi Yasumura
睦美 保村
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Yakult Honsha Co Ltd
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Yakult Honsha Co Ltd
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a vegetable beverage containing fermented milk, having good flavor and excellent quality stability without forming the flocculation/precipitation of a pulp portion derived from the vegetables and milk proteins even on preserving, and a method for producing the same. <P>SOLUTION: This vegetable beverage containing the fermented milk is obtained by mixing vegetable juice containing at least ≥1 kind of a tomato-processed food raw material selected from a group consisting of tomato juice, tomato puree and tomato paste, with the fermented milk and then homogenizing. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、はっ酵乳入り野菜飲料に関し、更に詳細には、野菜汁由来のパルプ分やはっ酵乳由来の乳蛋白等の凝集や沈殿を生じることがなく、品質安定性に優れ、且つ風味良好なはっ酵乳入り野菜飲料およびその製造方法に関する。   The present invention relates to a vegetable drink containing fermented milk, and more specifically, it does not cause aggregation or precipitation of pulp derived from vegetable juice or milk protein derived from fermented milk, and has excellent quality stability and good flavor. The present invention relates to a vegetable drink containing fermented milk and a method for producing the same.

野菜は、各種ビタミン、ミネラル等の栄養素や植物性繊維を豊富に含み、低カロリーであることから、健康指向型の食品として利用が拡大しており、更に、これを摂取し易いジュース(野菜汁)とすることも広く行われている。   Vegetables are rich in nutrients such as vitamins and minerals and plant fiber, and are low in calories, so their use is expanding as health-oriented foods. ) Is also widely performed.

一般に、野菜汁の多くは、青臭みや苦味などの好ましくない風味を有することから、これらを利用した食品の製造、例えば、野菜ジュースの製造においては、これらの課題を解決するために様々な手段が用いられている。具体的には、野菜搾汁液をプロテアーゼで処理する方法(特許文献1)、野菜搾汁液にトレハロースを添加する方法(特許文献2)、野菜汁を弱塩基性陰イオン交換樹脂で処理する方法(特許文献3)等を挙げることができる。   In general, many vegetable juices have unfavorable flavors such as a blue odor and bitterness. Therefore, in the production of foods using these, for example, the production of vegetable juices, there are various means for solving these problems. It is used. Specifically, a method of treating vegetable juice with protease (Patent Literature 1), a method of adding trehalose to vegetable juice (Patent Literature 2), and a method of treating vegetable juice with weakly basic anion exchange resin (Patent Literature 2) Patent document 3) etc. can be mentioned.

しかしながら、野菜搾汁液をプロテアーゼで処理する方法は、苦味の除去には効果があるものの青臭みの除去効果は充分でなく、また、野菜搾汁液にトレハロースを添加する方法についても、渋み・エグ味の軽減効果はあるが、やはり青臭みの除去効果は充分でない。   However, the method of treating vegetable juice with protease is effective in removing bitterness, but the effect of removing blue odor is not sufficient, and the method of adding trehalose to vegetable juice is also astringent and delicious. However, the blue odor removal effect is not sufficient.

また、野菜汁 を弱塩基性陰イオン交換樹脂で処理する方法は有効な方法ではあるものの、それなりの規模の装置が必要であり、経済性において問題のある方法である。   Moreover, although the method of treating vegetable juice with a weakly basic anion exchange resin is an effective method, it requires a device of a certain scale, and is a method with a problem in economy.

一方で、野菜汁に、はっ酵乳を混合するとマイルドな風味となり、飲みやすくなることは古くから知られており(特許文献4)、近年、同技術を利用した製品が上市されるようになってきている。   On the other hand, when fermented milk is mixed with vegetable juice, it has been known for a long time that it has a mild flavor and is easy to drink (Patent Document 4). In recent years, products using this technology have come to market. ing.

しかしながら、一般に、はっ酵乳を混合した飲料は保存すると乳蛋白質が凝集して沈降し、沈殿物が発生するという問題があることから、はっ酵乳を飲料等に利用する場合には、乳蛋白質の分散安定性を向上させる目的で、増粘安定剤を使用することが一般的に知られている(特許文献5および6)。   However, in general, when a beverage mixed with fermented milk is stored, there is a problem that the milk protein aggregates and settles and precipitates are generated. Therefore, when fermented milk is used for beverages, the milk protein dispersion It is generally known to use a thickening stabilizer for the purpose of improving stability (Patent Documents 5 and 6).

また、野菜汁に、はっ酵乳を混合した場合、乳蛋白質が野菜汁中のポリフェノールなどの成分と凝集することもあり、野菜汁を含まない、はっ酵乳入り飲料(乳性飲料等)よりも安定化は困難であった。更に、野菜汁の種類、パルプ物質の量、混合比率、製品比重などの条件によっても安定性が異なるという問題もある。この野菜汁にはっ酵乳を混合した場合の安定性を改善する方法としては、ジェランガムとペクチンおよび/又は大豆食物繊維を安定剤として使用する方法(特許文献7)が提案されている。   In addition, when fermented milk is mixed with vegetable juice, milk protein may aggregate with components such as polyphenols in vegetable juice, which is more stable than beverages containing fermented milk (such as dairy beverages) that do not contain vegetable juice. Conversion was difficult. Furthermore, there is also a problem that the stability varies depending on conditions such as the type of vegetable juice, the amount of pulp material, the mixing ratio, and the product specific gravity. As a method of improving the stability when fermented milk is mixed with this vegetable juice, a method of using gellan gum and pectin and / or soybean dietary fiber as a stabilizer has been proposed (Patent Document 7).

しかしながら、上記のように工夫したはっ酵乳入り野菜汁であっても、品質安定性が低く、保存した場合に野菜由来のパルプ分や乳蛋白質が凝集・沈殿を生じてしまい、商品価値が損なわれることがあった。   However, even if it is vegetable juice with fermented milk devised as described above, the quality stability is low, and when stored, pulp and milk proteins derived from vegetables cause aggregation and precipitation, and the commercial value is impaired. There was a thing.

特開平3−191767号公報Japanese Patent Laid-Open No. 3-191767 特開2000−116362号公報JP 2000-116362 A 特開平8−242826号公報JP-A-8-242826 特開昭62−36168号公報JP 62-36168 A 特開平8−56567号公報JP-A-8-56567 特開平9−94060号公報JP-A-9-94060 特開平11−262379号公報JP-A-11-262379

従って、本発明の課題は、良好な風味を有し、保存しても野菜由来のパルプ分や乳蛋白質が凝集・沈殿を生じることのない、優れた品質安定性を有するはっ酵乳を含有する野菜飲料およびその製造方法を提供することである。   Accordingly, an object of the present invention is to provide a vegetable containing fermented milk having excellent quality stability, which has a good flavor and does not cause aggregation or precipitation of vegetable-derived pulp or milk protein even when stored. It is to provide a beverage and a method for producing the same.

本発明者らは、上記課題を解決すべく鋭意検討を行った結果、トマト加工食品素材を含む野菜汁とはっ酵乳とを混合後、均質化して製品の粘度を調整することにより、長期間に渡って野菜汁由来のパルプ分や乳蛋白質の凝集・沈殿が生じることのない、品質安定性に優れ、且つ風味良好なはっ酵乳入り野菜飲料が得られることを見出し、本発明を完成した。   As a result of intensive studies to solve the above problems, the present inventors have mixed vegetable juice containing fermented tomato food material and fermented milk, and then homogenized and adjusted the viscosity of the product for a long period of time. The present invention was completed by finding that a vegetable drink containing fermented milk having excellent quality stability and good flavor without pulp or vegetable protein-derived aggregation or precipitation.

すなわち、本発明は、トマトジュース、トマトピューレおよびトマトペーストからなる群から選ばれたトマト加工食品素材の少なくとも1種以上を含む野菜汁と、はっ酵乳とを含み、これらを混合後、均質化して得られるものであることを特徴とするはっ酵乳入り野菜飲料である。   That is, the present invention includes vegetable juice containing at least one tomato processed food material selected from the group consisting of tomato juice, tomato puree and tomato paste, and fermented milk. It is a vegetable drink containing fermented milk characterized by being obtained.

また、本発明は、トマトジュース、トマトピューレおよびトマトペーストからなる群から選ばれたトマト加工食品素材の少なくとも1種以上を含む野菜汁と、はっ酵乳とを混合し、次いで均質化することを特徴とするはっ酵乳入り野菜飲料の製造方法である。   Further, the present invention is characterized in that a vegetable juice containing at least one tomato processed food material selected from the group consisting of tomato juice, tomato puree and tomato paste is mixed with fermented milk and then homogenized. It is a manufacturing method of vegetable drink containing fermented milk.

本発明のはっ酵乳入り野菜飲料は、長期間に渡って野菜汁由来のパルプ分や乳蛋白質の凝集・沈殿が生じることのない、品質安定性に優れ、且つ風味良好なものである。   The fermented milk-containing vegetable beverage of the present invention is excellent in quality stability and good in flavor without causing pulp or milk protein aggregation or precipitation from occurring over a long period of time.

従って、本発明のはっ酵乳入り野菜飲料は商品価値が高いものである。   Therefore, the fermented milk-containing vegetable drink of the present invention has a high commercial value.

本発明のはっ酵乳入り野菜飲料に使用される野菜汁は、トマトジュース、トマトピューレおよびトマトペーストから選ばれたトマト加工食品素材(以下、これらを単に「トマト加工食品素材」という)の少なくとも1種以上を必須成分として含有するものである。これらトマト加工食品素材としては日本農林規格(JAS)で定められたトマト加工品の中のトマトジュース、トマトピューレおよびトマトペーストの規格に適合するものを用いればよい。具体的にトマトジュースとはトマトを破砕後、パルパーやフィニッシャーで裏ごししたものをいう。また、トマトピューレとは、トマトを破砕後、裏ごしして濃縮したものであって、無塩可溶性固形分が24質量%(以下、単に「%」という)未満のものをいう。更に、トマトペーストとは、トマトを破砕後、裏ごしして高度に濃縮したもので、無塩可溶性固形分が24%以上のものをいう。本発明においては、上記規格を満足するものであれば、特に制限されることなく使用することが可能である。   The vegetable juice used in the vegetable drink containing fermented milk of the present invention is at least one kind of processed tomato food material selected from tomato juice, tomato puree and tomato paste (hereinafter simply referred to as “tomato processed food material”). The above is contained as an essential component. These tomato processed food materials may be those that comply with the standards for tomato juice, tomato puree and tomato paste in processed tomato products defined by the Japanese Agricultural Standards (JAS). Specifically, tomato juice refers to a tomato that has been crushed and then lined with a pulper or finisher. In addition, tomato puree refers to a tomato that has been crushed and then concentrated by lining and has a salt-free soluble solid content of less than 24% by mass (hereinafter simply referred to as “%”). Furthermore, tomato paste refers to a tomato that is highly concentrated by crushing the tomato after crushing and having a salt-free soluble solid content of 24% or more. In this invention, if it satisfies the said specification, it can be used without a restriction | limiting in particular.

また、上記野菜汁には、上記トマト加工食品素材の少なくとも1種以上を必須成分として含有するほかに、一般に野菜ジュース等で使用する各種野菜の搾汁液を含んでもよい。前記搾汁液の原料となる野菜としては、カボチャ、ピーマンなどの果菜類、キャベツ、ほうれんそう、レタス、パセリ、クレソン、小松菜などの葉菜類、ニンジン、大根、牛蒡などの根菜類、アスパラガス、セロリ、三つ葉などの茎菜類、ブロッコリー、カリフラワーなどの花菜類を挙げられる。これらの野菜は1種または2種以上を組み合わせてもよい。   Moreover, in addition to containing at least one kind of the tomato processed food material as an essential component, the vegetable juice may include juices of various vegetables generally used in vegetable juices and the like. Vegetables used as the raw material of the juice include fruit vegetables such as pumpkin and pepper, cabbage, spinach, leaf vegetables such as lettuce, parsley, watercress and komatsuna, root vegetables such as carrot, radish and beef bowl, asparagus, celery and trefoil. Stalk vegetables such as broccoli and cauliflower. These vegetables may combine 1 type (s) or 2 or more types.

前記の野菜を使用して搾汁液を製造する方法としては、特に制限されるわけではなく、常法に従って行えばよい。具体的には、野菜をブランチング処理した後、破砕し、搾汁する方法や、低温搾汁する方法などを挙げることができるが、得られる搾汁液の酸化による品質劣化を防止するため、野菜をアスコルビン酸存在下、低温で破砕・搾汁し、これを加熱して生体酵素を失活させる方法を用いることが好ましい。   The method for producing the juice using the above vegetables is not particularly limited, and may be performed according to a conventional method. Specifically, after blanching the vegetable, it can be crushed and squeezed, a method of cold squeezing, etc. In order to prevent quality deterioration due to oxidation of the resulting juice, It is preferable to use a method of crushing and squeezing the juice at low temperature in the presence of ascorbic acid and heating it to deactivate the biological enzyme.

また、複数種の野菜を用いて野菜汁を製造する場合には、各野菜を個別に処理して搾汁液を調製した後、これらにトマト加工食品素材を加え混合してもよく、また、複数種の野菜を一緒に処理して搾汁液を調製し、これとトマト加工食品素材とを混合して調製してもよい。   Moreover, when manufacturing vegetable juice using several kinds of vegetables, after processing each vegetable separately and preparing squeezed liquid, you may add and process tomato processed food material to these, It may be prepared by processing seed vegetables together to prepare a juice, and mixing this with a tomato processed food material.

本発明のはっ酵乳入り野菜飲料において、野菜汁の配合量は、製品あたりのトマト加工食品素材の含有量が、ストレート換算の重量で15%〜50%、より好ましくは、20%〜40%となるように設定することが好ましい。また、製品あたりのトマト加工食品素材の含有量が、ストレート換算で15%よりも少ないと、後述するように、はっ酵乳を配合して、均質化処理した場合であっても野菜汁由来のパルプ分や乳蛋白質の凝集・沈殿を抑制する効果が十分に得られず、結果として製品の品質劣化を招くこととなるため好ましくない。また、製品あたりのトマト加工食品素材の含有量がストレート換算の重量比で50%よりも多くなると、はっ酵乳を配合して均質化することにより、必要以上に製品の粘度が高くなってしまい、食感(のど越し)や風味を著しく損なうため、好ましくない。なお、本発明におけるトマト加工食品素材のストレート換算とは、ストレートトマト(濃縮していないもの)の無塩可溶性固形分を4.5%として、トマト加工食品素材の質量をストレートトマトの質量に換算することである。   In the vegetable drink containing fermented milk of the present invention, the content of the vegetable juice is 15% to 50%, more preferably 20% to 40%, in terms of the weight in terms of straight, the content of the tomato processed food material per product. It is preferable to set so that Also, if the content of processed tomato food material per product is less than 15% on a straight basis, the pulp derived from vegetable juice, even when blended with fermented milk and homogenized as described later The effect of suppressing the aggregation and precipitation of milk and milk proteins cannot be sufficiently obtained, and as a result, the quality of the product is deteriorated. Moreover, when the content of the processed tomato food material per product is more than 50% in a weight ratio in terms of straight, by blending and homogenizing the fermented milk, the viscosity of the product becomes higher than necessary, This is not preferable because the texture (over the throat) and flavor are significantly impaired. In addition, the straight conversion of the processed tomato food material in the present invention means that the straight salted tomato (non-concentrated) non-salt soluble solid content is 4.5%, and the mass of the processed tomato food material is converted to the straight tomato mass. It is to be.

一方、本発明のはっ酵乳入り野菜飲料に使用されるはっ酵乳は、乳等省令で定められているはっ酵乳に該当するものであれば特に制限されない。すなわち、はっ酵乳としては、牛乳、山羊乳等の生乳、脱脂粉乳、全脂粉乳、生クリーム等の乳製品をそのまま或いは必要に応じて希釈した溶液(原料乳)中で、乳酸菌やビフィドバクテリウム属細菌などを培養した溶液を使用することができる。   On the other hand, the fermented milk used for the vegetable drink containing fermented milk of the present invention is not particularly limited as long as it corresponds to the fermented milk specified by the Ministerial Ordinance. That is, as fermented milk, dairy products such as milk, goat milk, skim milk powder, whole milk powder, fresh cream and other dairy products can be used as they are or in a solution (raw milk) diluted as necessary. A solution obtained by culturing a bacterium belonging to the genus Plum can be used.

ここで、はっ酵乳の製造に用いられる乳酸菌やビフィドバクテリウム属細菌としては、通常、食品製造に使用される微生物であれば特に限定されず、例えば、ラクトバチルス・カゼイ、ラクトバチルス・アシドフィルス、ラクトバチルス・ヘルベティカス、ラクトバチルス・ガセリ、ラクトバチルス・ファーメンタム、ラクトバチルス・サリバリウス、ラクトバチルス・ユーグルティ、ラクトバチルス・デルブルッキィ サブスピーシーズ.ブルガリカス、ラクトバチルス・ジョンソニー等のラクトバチルス属細菌、ストレプトコッカス・サーモフィルス等のストレプトコッカス属細菌、ラクトコッカス・ラクチス サブスピーシーズ.ラクチス、ラクトコッカス・ラクチス サブスピーシーズ.クレモリス、ラクトコッカス・プランタラム、ラクトコッカス・ラフィノラクチス等のラクトコッカス属細菌、エンテロコッカス・フェカーリス、エンテロコッカス・フェシウム等のエンテロコッカス属細菌、ビフィドバクテリウム・ブレーベ、ビフィドバクテリウム・ロンガム、ビフィドバクテリウム・インファンティス、ビフィドバクテリウム・アドレスセンティス、ビフィドバクテリウム・ビフィダム、ビフィドバクテリウム・カテヌラータム、ビフィドバクテリウム・シュードカテヌラータム、ビフィドバクテリウム・アングラータム、ビフィドバクテリウム・ラクチス、ビフィドバクテリウム・アニマリス等のビフィドバクテリウム属細菌を挙げることができる。なお、これらの乳酸菌やビフィドバクテリウム属細菌は、単独で用いても、あるいは2種以上を併用してもよい。   Here, as a lactic acid bacterium or Bifidobacterium genus bacteria used for the production of fermented milk, it is not particularly limited as long as it is a microorganism usually used for food production, for example, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus helveticus, Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus eugleti, Lactobacillus delbrukii Subspecies. Lactobacillus bacteria such as Bulgaricus and Lactobacillus johnsonii, Streptococcus bacteria such as Streptococcus thermophilus, Lactococcus lactis Subspecies. Lactis, Lactococcus lactis Subspecies. Lactococcus bacteria such as Cremoris, Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus bacteria such as Enterococcus faecium, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium Um Infantitis, Bifidobacterium addresscentis, Bifidobacterium bifidum, Bifidobacterium catenulatum, Bifidobacterium pseudocatenulatum, Bifidobacterium angulatam, Bifidobacterium -Bifidobacterium genus bacteria, such as lactis and Bifidobacterium animalis, can be mentioned. In addition, these lactic acid bacteria and Bifidobacterium bacteria may be used independently or may use 2 or more types together.

上記微生物を原料乳に作用させるための条件や発酵方法は、通常のはっ酵乳の製造に使用される条件および方法を適用すればよく、特に限定されない。例えば、微生物の発酵条件としては、30〜40℃の温度で、pHが3.5〜5.5になるまで発酵させる等の条件が挙げられ、発酵方法としては、静置発酵、攪拌発酵、振盪発酵、通気発酵等から発酵に用いる微生物に適したものを適宜選択する方法が挙げられる。   The conditions and the fermentation method for allowing the microorganisms to act on the raw milk are not particularly limited as long as the conditions and methods used for the production of ordinary fermented milk are applied. For example, conditions for fermentation of microorganisms include conditions such as fermentation at a temperature of 30 to 40 ° C. until the pH reaches 3.5 to 5.5. Fermentation methods include stationary fermentation, stirred fermentation, The method of selecting suitably the microorganisms used for fermentation from shaking fermentation, aeration fermentation, etc. is mentioned.

本発明のはっ酵乳入り野菜飲料におけるはっ酵乳の配合量は、製品あたり、無脂乳固形分として0.1%〜2.5%程度、好ましくは0.4%〜1.6%程度とするのがよい。製品あたりのはっ酵乳の配合量が、無脂乳固形分として0.1%よりも少ないとはっ酵乳入り野菜飲料に特有のまろやかなで飲みやすい風味を与えることができず、また、2.5%よりも多くなると、乳蛋白質の凝集・沈殿を生じる可能性があるため、好ましくない。   The blended amount of the fermented milk in the vegetable drink with fermented milk of the present invention is about 0.1% to 2.5%, preferably about 0.4% to 1.6% as non-fat milk solid content per product. It is good. If the blended amount of fermented milk per product is less than 0.1% as the solid content of non-fat milk, the mellow and easy-to-drink flavor characteristic of vegetable drinks with fermented milk cannot be given, and 2.5 If it exceeds 50%, milk protein aggregation and precipitation may occur, which is not preferable.

また、本発明のはっ酵乳入り野菜飲料には、より好ましい風味とするため、果汁を添加することができる。ここで、配合される果汁としては、一般的な果汁であれば特に制限されることなく使用することができ、例えば、オレンジ、レモン、ライム、パイナップル、アップル、マスカット、グレープフルーツから選ばれた果実の少なくとも1種以上含有する果汁を挙げることができるが、特に、風味の面でトマトとの相性がよいアップルを主体とする果汁を使用することが好ましい。   Moreover, in order to make it a more preferable flavor to the vegetable drink containing fermented milk of this invention, fruit juice can be added. Here, as a mixed fruit juice, it can be used without particular limitation as long as it is a general fruit juice, for example, a fruit selected from orange, lemon, lime, pineapple, apple, muscat, grapefruit. Examples include fruit juices containing at least one kind, and it is particularly preferable to use fruit juices mainly composed of apples that are compatible with tomatoes in terms of flavor.

本発明のはっ酵乳入り野菜飲料にこれらの果汁を配合する場合、その配合量は、製品あたりストレート換算の質量で10%〜70%、より好ましくは、30%〜70%程度とすればよい。なお、本発明における果汁のストレート換算とは、果実飲料の日本農林規格第24条第1項の規格の表示の方法の1の(1)に準じたものである。   When these fruit juices are blended with the fermented milk-containing vegetable beverage of the present invention, the blending amount may be 10% to 70%, more preferably about 30% to 70% in terms of mass per product. In addition, the straight conversion of the fruit juice in this invention is based on 1 (1) of the display method of the specification of Japanese agricultural and forestry standard article 24 Clause 1 of a fruit drink.

本発明のはっ酵乳入り野菜飲料は、上述のトマト加工食品素材の少なくとも1種以上を含む野菜汁とはっ酵乳を混合後、これを均質化処理する以外は通常の野菜飲料の製造方法により製造することができる。すなわち、本発明のはっ酵乳入り野菜飲料は、上述のトマト加工食品素材の少なくとも1種以上を含む野菜汁とはっ酵乳に、必要に応じて果汁や任意の成分を添加し、pH調整等を行った後、混合し、均質化処理する。次いで、均質化処理したものをプレート式熱交換器等で加熱殺菌し、PETボトル等の容器に充填して製品化する。なお、上記製造方法において均質化処理は、トマト加工食品素材の少なくとも1種以上を含有する野菜汁とはっ酵乳とをそれぞれ別個に均質化処理した後に両者を混合した場合には、本発明が課題とする品質安定性を十分に満足することができないため好ましくない。   The vegetable drink containing fermented milk of the present invention is produced by a normal method for producing a vegetable drink, except that after mixing vegetable juice containing fermented milk and fermented milk containing at least one of the above-mentioned processed tomato processed food materials, this is homogenized. be able to. That is, the fermented milk-containing vegetable beverage of the present invention adds fruit juice and optional components to the vegetable juice and fermented milk containing at least one of the above-described processed tomato food materials, and performs pH adjustment as necessary. Then, mix and homogenize. Next, the homogenized material is heat sterilized with a plate heat exchanger or the like, and filled into a container such as a PET bottle to produce a product. In the above production method, the homogenization treatment is performed when the vegetable juice containing at least one tomato processed food material and the fermented milk are separately homogenized and then mixed together. It is not preferable because the quality stability cannot be sufficiently satisfied.

上記製造方法において均質化処理とは、液体分散媒中に存在する乳濁物質、粒子状物質を機械的に破砕し、液分離(分離浮上、沈殿等)を抑え、均一な乳化状態を作り出す操作、いわゆる「ホモジナイズ」をいうが、本発明においての均質化処理とは、見かけ上均質になり固液分離を生じない状態をいう。なお、本発明において、均質化処理は、機械的分散により物理的に均質化できる方法であれば、特に制限されることなく行うことができるが、操作上、バルブ式ホモジナイザーを使用して行うことが好ましい。   In the above manufacturing method, homogenization is the operation of mechanically crushing emulsion substances and particulate substances present in the liquid dispersion medium to suppress liquid separation (separation, flotation, precipitation, etc.) and create a uniform emulsified state. The so-called “homogenization” refers to a state in which homogenization treatment in the present invention is apparently homogeneous and does not cause solid-liquid separation. In the present invention, the homogenization treatment can be performed without any particular limitation as long as it can be physically homogenized by mechanical dispersion, but in operation, it is performed using a valve-type homogenizer. Is preferred.

上記均質化処理において、均質化処理する際の圧力は、使用する野菜汁、はっ酵乳の配合量などによって異なるため、実験的に確かめて設定することが好ましいが、得られるはっ酵乳入り野菜飲料の製品粘度が、30〜150mPa・s、好ましくは50〜110mPa・sとなるように、およそ2〜15MPa程度とすればよい。均質化処理により、製品粘度が30mPa・sよりも低くなると、野菜汁由来のパルプ分や乳蛋白質の凝集・沈殿が生じやすくなり、保存による品質の劣化を招くことから好ましくない。また逆に、製品粘度が、150mPa・sよりも大きくなると、製品の粘度が高くなりすぎて食感(テクスチャー)を著しく損なうとともに風味も劣化するため、好ましくない。   In the above homogenization treatment, the pressure at the time of homogenization treatment varies depending on the vegetable juice used, the blended amount of fermented milk, etc., so it is preferable to confirm it experimentally, but the obtained fermented milk-containing vegetable beverage The product viscosity may be about 2 to 15 MPa so that the product viscosity is 30 to 150 mPa · s, preferably 50 to 110 mPa · s. If the product viscosity is lower than 30 mPa · s by the homogenization treatment, it is not preferable because pulp and vegetable protein-derived pulp and milk protein are easily aggregated and precipitated, resulting in deterioration of quality due to storage. On the other hand, if the product viscosity is higher than 150 mPa · s, the product viscosity becomes too high, the texture (texture) is remarkably impaired and the flavor is deteriorated.

本発明のポイントは均質化することによって、トマトパルプを破砕して、パルプに含まれるペクチン等の粘性物質を適度に溶出させ、飲料の粘度を適当な範囲に調整するところにある。すなわち、トマトパルプからの粘性物質の溶出が少ないと飲料の粘度増加が小さく、保存中の乳蛋白質の沈殿を抑制できないばかりでなく、トマトパルプの沈降も生じる。逆に、トマトパルプからの粘性物質の溶出が多いと飲料の粘度増加が大きくなり、重たいテクスチャーの飲みにくい飲料となる。   The point of the present invention is that the tomato pulp is crushed by homogenization, and viscous substances such as pectin contained in the pulp are appropriately eluted to adjust the viscosity of the beverage to an appropriate range. That is, if there is little elution of the viscous substance from tomato pulp, the increase in the viscosity of the beverage will be small, and not only can the precipitation of milk protein during storage be suppressed, but also the sedimentation of tomato pulp will occur. On the contrary, if there is much elution of the viscous substance from tomato pulp, the increase in the viscosity of the beverage will increase, resulting in a beverage that is difficult to drink with a heavy texture.

なお、本発明における製品粘度は、B型粘度計(BL型)によって測定した値を意味する(試料容器:500mlビーカー(標準タイプ)、測定温度20±1℃、ローター:No.1、ローターの回転速度:30rpm、測定値:測定開始20秒後(ローターが10回転したとき)の値)。   The product viscosity in the present invention means a value measured by a B-type viscometer (BL type) (sample container: 500 ml beaker (standard type), measurement temperature 20 ± 1 ° C., rotor: No. 1, rotor Rotational speed: 30 rpm, measured value: 20 seconds after the start of measurement (when the rotor has rotated 10 times).

本発明のはっ酵乳入り野菜飲料に添加される前記以外の任意の成分としては、通常各種飲食品へ配合される各種食品素材、例えば、各種糖質、増粘剤、乳化剤、各種ビタミン剤等の任意成分が挙げられる。これらの食品素材として具体的なものとしては、ショ糖、グルコース、フルクトース、パラチノース、トレハロース、ラクトース、キシロース、麦芽糖等の糖質、ソルビトール、キシリトール、エリスリトール、ラクチトール、パラチニット、還元水飴、還元麦芽糖水飴等の糖アルコール、アスパルテーム、ソーマチン、スクラロース、アセスルファムK、ステビア等の高甘味度甘味料、寒天、ゼラチン、カラギーナン、グァーガム、キサンタンガム、ペクチン、ローカストビーンガム、ジェランガム、カルボキシメチルセルロース、大豆多糖類、アルギン酸プロピレングリコール等の各種増粘(安定)剤、ショ糖脂肪酸エステル、グリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、ソルビタン脂肪酸エステル、レシチン等の乳化剤、クリーム、バター、サワークリームなどの乳脂肪、クエン酸、乳酸、酢酸、リンゴ酸、酒石酸、グルコン酸等の酸味料、ビタミンA、ビタミンB類、ビタミンC、ビタミンE類等の各種ビタミン類、カルシウム、マグネシウム、亜鉛、鉄、マンガン等のミネラル分、ヨーグルト系、ベリー系、オレンジ系、花梨系、シソ系、シトラス系、アップル系、ミント系、グレープ系、アプリコット系、ペア、カスタードクリーム、ピーチ、メロン、バナナ、トロピカル、ハーブ系、紅茶、コーヒー系等のフレーバー類が挙げられる。   As optional ingredients other than the above that are added to the vegetable drink containing fermented milk of the present invention, various food materials that are usually blended into various foods and beverages, such as various sugars, thickeners, emulsifiers, various vitamins, etc. Optional components are mentioned. Specific examples of these food materials include sugars such as sucrose, glucose, fructose, palatinose, trehalose, lactose, xylose, maltose, sorbitol, xylitol, erythritol, lactitol, palatinit, reduced starch syrup, reduced maltose starch syrup, etc. High-sweetness sweeteners such as sugar alcohol, aspartame, thaumatin, sucralose, acesulfame K, stevia, agar, gelatin, carrageenan, guar gum, xanthan gum, pectin, locust bean gum, gellan gum, carboxymethylcellulose, soy polysaccharide, propylene glycol alginate Various thickeners (stabilizers) such as sucrose fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, lecithin and other emulsifiers Milk fat such as cream, butter, sour cream, acidulants such as citric acid, lactic acid, acetic acid, malic acid, tartaric acid, gluconic acid, various vitamins such as vitamin A, vitamin B, vitamin C, vitamin E, calcium, Minerals such as magnesium, zinc, iron, manganese, yogurt, berry, orange, quince, perilla, citrus, apple, mint, grape, apricot, pair, custard cream, peach, melon , Banana, tropical, herbal, tea and coffee flavors.

以下、本発明を実施例を挙げて更に詳細に説明するが、本発明はこれらの実施例等になんら制約されるものではない。   EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further in detail, this invention is not restrict | limited at all by these Examples.

実 施 例 1
はっ酵乳入り野菜飲料の製造(1):
表1の処方で調合したものを、バルブ式ホモジナイザーを用いて5MPaの圧力で均質化処理した。次いで、これを90℃に加熱後、ガラス壜透明容器に熱時充填し、冷却してはっ酵乳入り野菜飲料を得た。このはっ酵乳入り野菜飲料を室温に2週間保存して沈殿を観察し、同時に風味の評価も行った。これらの結果を表2に示した。
Example 1
Manufacture of vegetable drink with fermented milk (1):
What was prepared by the formulation shown in Table 1 was homogenized at a pressure of 5 MPa using a valve homogenizer. Subsequently, after heating this to 90 degreeC, it filled with the glass bottle transparent container at the time of heating, cooled, and obtained the vegetable drink containing fermented milk. This fermented milk-containing vegetable drink was stored at room temperature for 2 weeks, and the precipitation was observed. At the same time, the flavor was also evaluated. These results are shown in Table 2.

Figure 2007061060
*1:無塩可溶性固形分18%
*2:Bx糖度36°
*3:ストレート汁(ほうれんそう、ピーマン、アスパラガス、ブロッコリー、
パセリ、クレソン、ラディッシュ、小松菜、レタス)
*4:無脂乳固形分8%(使用乳酸菌:ラクトバチルス・カゼイ)
*5:SM−YT(三栄源エフ・エフ・アイ(株))
*6:製品pHを3.8に調整する量
Figure 2007061060
* 1: 18% salt-free soluble solids
* 2: Bx sugar content 36 °
* 3: Straight juice (spinach, green pepper, asparagus, broccoli,
Parsley, watercress, radish, komatsuna, lettuce)
* 4: Non-fat milk solid content 8% (Lactic acid bacteria used: Lactobacillus casei)
* 5: SM-YT (Saneigen FFI Co., Ltd.)
* 6: Amount to adjust product pH to 3.8

Figure 2007061060
Figure 2007061060

表2に示したとおり、実施品Aおよび比較品Bはともに、はっ酵乳の添加によって野菜臭さが軽減されていたが、沈殿の発生状況は異なり、トマトピューレを含む実施品Aは保存後も沈殿がほとんどなかったが、人参濃縮汁を含む(トマトピューレを含まない)比較品Bは保存後の沈殿が多く認められた。   As shown in Table 2, both the execution product A and the comparison product B had a reduced vegetable odor due to the addition of fermented milk, but the occurrence of precipitation was different, and the execution product A containing tomato puree remained after storage. Although there was almost no precipitation, comparative product B containing ginseng juice (not containing tomato puree) showed a lot of precipitation after storage.

実 施 例 2
はっ酵乳入り野菜飲料の製造(2):
表3の処方で調合したものを、バルブ式ホモジナイザーを用いて5MPa(2次圧)の圧力で均質化処理した。次いで、チューブ式熱交換器で110℃、5秒間の殺菌を行い、滅菌したガラス壜透明容器に無菌充填してはっ酵乳入り野菜飲料を得た。このはっ酵乳入り野菜飲料の充填製品を室温に2週間保存して沈殿を観察し、同時に風味の評価も行った。なお、沈殿と風味は以下の基準に従い評価した。これらの結果を表4に示した。
Example 2
Manufacture of vegetable drink with fermented milk (2):
What was prepared with the formulation of Table 3 was homogenized at a pressure of 5 MPa (secondary pressure) using a valve-type homogenizer. Next, the tube-type heat exchanger was sterilized at 110 ° C. for 5 seconds, and aseptically filled into a sterilized glass bottle transparent container to obtain a vegetable drink containing fermented milk. The filled product of the vegetable drink containing fermented milk was stored at room temperature for 2 weeks, the precipitate was observed, and the flavor was also evaluated at the same time. In addition, precipitation and flavor were evaluated according to the following criteria. These results are shown in Table 4.

<沈殿評価基準>
(評価) (内容)
◎ : 沈殿なし
○ : 沈殿ほとんどなし
△ : 沈殿少しあり
× : 沈殿多い
<Precipitation evaluation criteria>
(Evaluation) (Content)
◎: No precipitation ○: Little precipitation △: There is little precipitation ×: Many precipitations

<風味評価基準>
(評価) (内容)
◎ : 良好
○ : やや粘度感があるが、野菜臭さが少なく、飲みやすい
× : トマト感または粘度感が強すぎる
<Flavor evaluation criteria>
(Evaluation) (Content)
◎: Good ○: Slightly viscous, but has little vegetable odor and easy to drink ×: Tomato or viscosity is too strong

Figure 2007061060
*1:無塩可溶性固形分28%
*2:ストレート汁(ほうれんそう、ピーマン、アスパラガス、ブロッコリー、パセリ、
クレソン、ラディッシュ、小松菜、レタス)
*3:無脂乳固形分20%(使用乳酸菌:ストレプトコッカス・サーモフィルス)
*4:Bx糖度70°
*5:SM−YT(三栄源エフ・エフ・アイ(株))
*6:製品のpHを3.9に調整するための量
Figure 2007061060
* 1: 28% salt-free soluble solids
* 2: Straight juice (spinach, peppers, asparagus, broccoli, parsley,
Watercress, radish, komatsuna, lettuce)
* 3: 20% non-fat milk solids (Lactic acid bacteria used: Streptococcus thermophilus)
* 4: Bx sugar content 70 °
* 5: SM-YT (Saneigen FFI Co., Ltd.)
* 6: Amount to adjust product pH to 3.9

Figure 2007061060
Figure 2007061060

表4から明らかなとおり、均質化処理を施しても、製品の粘度が30mPa・sよりも低い場合には、十分に沈殿を抑制する効果を得ることはできないが、製品粘度が高くなる(具体的には、30mPa・s以上)と沈殿を抑制する効果が得られることが認められた。また、一方で、製品の風味に関しては、トマト含量に依存する傾向が認められ、トマト含量が50%よりも多くなると風味が悪くなった。   As is apparent from Table 4, even when the homogenization treatment is performed, if the product viscosity is lower than 30 mPa · s, the effect of suppressing precipitation cannot be obtained sufficiently, but the product viscosity increases (specifically Specifically, it was confirmed that the effect of suppressing precipitation was obtained with 30 mPa · s or more. On the other hand, with respect to the flavor of the product, a tendency to depend on the tomato content was recognized, and when the tomato content was more than 50%, the flavor became worse.

実 施 例 3
はっ酵乳入り野菜飲料の製造(3):
均質化処理を15MPa(1次圧:10MPa、2次圧:5Mpa)で行う以外は、実施例2と同様にしてはっ酵乳入り野菜飲料を得た。このはっ酵乳入り野菜飲料の充填製品を室温に2週間保存して沈殿と風味を実施例2と同様に評価した。これらの結果を表5に示した。
Example 3
Manufacture of vegetable drink with fermented milk (3):
A vegetable drink containing fermented milk was obtained in the same manner as in Example 2 except that the homogenization treatment was performed at 15 MPa (primary pressure: 10 MPa, secondary pressure: 5 Mpa). The filled product of the fermented milk-containing vegetable drink was stored at room temperature for 2 weeks, and the precipitation and flavor were evaluated in the same manner as in Example 2. These results are shown in Table 5.

Figure 2007061060
Figure 2007061060

表5から明らかなとおり、均質化処理の条件を変えても、製品中の沈殿を抑制する効果は、表4の結果と同様の傾向が認められた。また、均質化条件を厳しく(均質化圧力を高く)設定した場合には、トマト含量が30%よりも多くなると風味が悪くなった。   As is apparent from Table 5, even when the conditions for the homogenization treatment were changed, the same tendency as the results in Table 4 was observed for the effect of suppressing precipitation in the product. In addition, when the homogenization conditions were set strictly (the homogenization pressure was high), the flavor deteriorated when the tomato content was higher than 30%.

実 施 例 4
はっ酵乳入り野菜飲料の製造(4):
実施例2の実施品4と同じ処方で調合したものを、バルブ式ホモジナイザーを用いて表5に示した各圧力で均質化処理した後、チューブ式熱交換器で110℃で5秒間の殺菌を行い、滅菌したガラス壜透明容器に無菌充填してはっ酵乳入り野菜飲料を得た。このはっ酵乳入り野菜飲料の充填製品を室温に2週間保存して沈殿と風味を実施例2と同様に評価した。これらの結果を表6に示した。
Example 4
Manufacture of vegetable drink with fermented milk (4):
A mixture prepared with the same formulation as Example 4 of Example 2 was homogenized at each pressure shown in Table 5 using a valve homogenizer, and then sterilized at 110 ° C. for 5 seconds with a tube heat exchanger. This was performed and aseptically filled into a sterilized glass bottle transparent container to obtain a vegetable drink containing fermented milk. The filled product of the fermented milk-containing vegetable drink was stored at room temperature for 2 weeks, and the precipitation and flavor were evaluated in the same manner as in Example 2. These results are shown in Table 6.

Figure 2007061060
Figure 2007061060

表6に示したとおり、均質化圧力を2〜15MPaの条件に設定した場合には、風味が良好で沈殿も少ないはっ酵乳入り野菜飲料が得られた。実施例2および実施例3の結果(表4および表5)と合わせて考えると、製品粘度を30〜150mPa・sになるように均質化圧力を設定したときに風味良好で沈殿の少ない製品が得られる。   As shown in Table 6, when the homogenization pressure was set to 2 to 15 MPa, a vegetable drink containing fermented milk with good flavor and little precipitation was obtained. Considering together with the results of Examples 2 and 3 (Tables 4 and 5), when the homogenization pressure was set so that the product viscosity was 30 to 150 mPa · s, a product with good flavor and less precipitation was obtained. can get.

実 施 例 5
はっ酵乳入り野菜飲料の製造(5):
表7の処方で調合したものを、バルブ式ホモジナイザーを用いて5MPaで均質化処理した。次いで、チューブ式熱交換器で110℃、5秒間の殺菌を行い、滅菌したガラス壜透明容器に無菌充填し、はっ酵乳入り野菜飲料(実施品C、DおよびE)を得た。これらのはっ酵乳入り野菜飲料は野菜の青臭みがなく良好な風味であり、室温で2週間保存後も沈殿などの外観変化は認められなかった。
Example 5
Manufacture of vegetable drink with fermented milk (5):
What was prepared with the formulation of Table 7 was homogenized at 5 MPa using a valve-type homogenizer. Next, the tube-type heat exchanger was sterilized at 110 ° C. for 5 seconds, and aseptically filled into a sterilized glass bottle transparent container to obtain a fermented milk-containing vegetable beverage (practical products C, D, and E). These fermented milk-containing vegetable beverages have a good flavor without the blue smell of vegetables, and no change in appearance such as precipitation was observed after storage at room temperature for 2 weeks.

Figure 2007061060
*1:無塩可溶性固形分28%
*2:無塩可溶性固形分18%
*3:Bx糖度36°
*4:ほうれんそう、ピーマン、アスパラガス、ブロッコリー、パセリ、クレソン、
ラディッシュ、小松菜、レタス
*5:無脂乳固形分20%(使用乳酸菌:ラクトバチルス・カゼイ及びストレプト
コッカス・サーモフィルス)
*6:無脂乳固形分8%(使用乳酸菌:ラクトバチルス・カゼイ及びストレプトコッ
カス・サーモフィルス)
*7:製品のpHを3.9に調整する量
*8:SM−YT(三栄源エフ・エフ・アイ(株))
Figure 2007061060
* 1: 28% salt-free soluble solids
* 2: 18% salt-free soluble solids
* 3: Bx sugar content 36 °
* 4: Spinach, green pepper, asparagus, broccoli, parsley, watercress,
Radish, Japanese mustard spinach, lettuce * 5: Non-fat milk solid content 20% (Lactic acid bacteria used: Lactobacillus casei and Strept
Coccus thermophilus)
* 6: Non-fat milk solids 8% (Lactic acid bacteria used: Lactobacillus casei and Streptococcus
Cas thermophilus)
* 7: Amount to adjust product pH to 3.9 * 8: SM-YT (San-Eigen FFI Co., Ltd.)

本発明のはっ酵乳入り野菜飲料は、野菜由来の各種ビタミン、ミネラル等の栄養素や植物性繊維を豊富に含むので、広く健康増進に利用することができる。
Since the vegetable drink containing fermented milk of the present invention contains abundant nutrients such as various vitamins and minerals derived from vegetables and vegetable fibers, it can be widely used for health promotion.

Claims (7)

トマトジュース、トマトピューレおよびトマトペーストからなる群から選ばれたトマト加工食品素材の少なくとも1種以上を含む野菜汁と、はっ酵乳と含み、これらを混合後、均質化して得られるものであることを特徴とするはっ酵乳入り野菜飲料。   It contains vegetable juice containing at least one kind of processed tomato food material selected from the group consisting of tomato juice, tomato puree and tomato paste, fermented milk, and is obtained by mixing and homogenizing these. A featured vegetable drink with fermented milk. はっ酵乳の配合量が、無脂乳固形分として0.1質量%〜2.5質量%である請求項1に記載のはっ酵乳入り野菜飲料。   The vegetable drink containing fermented milk according to claim 1, wherein the blended amount of the fermented milk is 0.1% by mass to 2.5% by mass as the solid content of non-fat milk. 製品の粘度が、30〜150mPa・sである請求項1または2に記載のはっ酵乳入り野菜飲料。   The vegetable drink containing fermented milk according to claim 1 or 2, wherein the product has a viscosity of 30 to 150 mPa · s. 更に、果汁を含むものである請求項1〜3のいずれかに記載のはっ酵乳入り野菜飲料。   Furthermore, the vegetable drink containing fermented milk in any one of Claims 1-3 which contains fruit juice. 果汁の配合量が、ストレート換算で30質量%〜70質量%である請求項1〜4のいずれかに記載のはっ酵乳入り野菜飲料。   The blended amount of fruit juice is 30% by mass to 70% by mass in terms of straight, The vegetable drink with fermented milk according to any one of claims 1 to 4. 容器入りである請求項第1〜5のいずれかに記載のはっ酵乳入り野菜飲料。   The vegetable drink containing fermented milk according to any one of claims 1 to 5, which is contained in a container. トマトジュース、トマトピューレおよびトマトペーストからなる群から選ばれたトマト加工食品素材の少なくとも1種以上を含む野菜汁と、はっ酵乳とを混合し、次いで均質化することを特徴とするはっ酵乳入り野菜飲料の製造方法。
Vegetable juice containing fermented milk, characterized in that vegetable juice containing at least one tomato processed food material selected from the group consisting of tomato juice, tomato puree and tomato paste is mixed with fermented milk and then homogenized A method for producing a beverage.
JP2005254308A 2005-09-02 2005-09-02 Vegetable beverage containing fermented milk and method for producing the same Pending JP2007061060A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009077641A (en) * 2007-09-26 2009-04-16 Manns Wine Co Ltd Turbid alcoholic beverage
JP2009284897A (en) * 2008-11-12 2009-12-10 Ito En Ltd Milk protein-containing packaged vegetable drink
JPWO2009014253A1 (en) * 2007-07-26 2010-10-07 味の素株式会社 Method for producing a frozen beverage comprising a plant containing a capsinoid compound
JP2012524549A (en) * 2009-04-24 2012-10-18 ネステク ソシエテ アノニム Storage stable fermented dairy product and method for producing the same
CN103783161A (en) * 2014-02-21 2014-05-14 李海燕 Preparation method of houseleek active lactobacillus beverage
JP2015226521A (en) * 2014-06-02 2015-12-17 キッコーマン株式会社 Protein-containing beverage
CN109820040A (en) * 2017-11-23 2019-05-31 内蒙古伊利实业集团股份有限公司 A kind of viable type fruits and vegetables juice milk drink and preparation method thereof

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2009014253A1 (en) * 2007-07-26 2010-10-07 味の素株式会社 Method for producing a frozen beverage comprising a plant containing a capsinoid compound
JP2009077641A (en) * 2007-09-26 2009-04-16 Manns Wine Co Ltd Turbid alcoholic beverage
JP2009284897A (en) * 2008-11-12 2009-12-10 Ito En Ltd Milk protein-containing packaged vegetable drink
JP2012524549A (en) * 2009-04-24 2012-10-18 ネステク ソシエテ アノニム Storage stable fermented dairy product and method for producing the same
CN103783161A (en) * 2014-02-21 2014-05-14 李海燕 Preparation method of houseleek active lactobacillus beverage
JP2015226521A (en) * 2014-06-02 2015-12-17 キッコーマン株式会社 Protein-containing beverage
CN109820040A (en) * 2017-11-23 2019-05-31 内蒙古伊利实业集团股份有限公司 A kind of viable type fruits and vegetables juice milk drink and preparation method thereof

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