JP2007051073A - Dna salt and functional polymer film obtained by forming the same salt into film - Google Patents
Dna salt and functional polymer film obtained by forming the same salt into film Download PDFInfo
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本発明は、新規な機能性高分子、詳しくはＤＮＡリン酸アニオンと、長鎖アルキルを有する４級アンモニウムカチオンとの塩、およびこの塩から成膜してなる機能性高分子膜に関する。 The present invention relates to a novel functional polymer, specifically, a salt of a DNA phosphate anion and a quaternary ammonium cation having a long-chain alkyl, and a functional polymer film formed from this salt.
ＤＮＡはリン酸アニオンを有する巨大分子である。このリン酸アニオンへカチオン性両親媒性分子をイオン結合し、両親媒性ＤＮＡ塩を製造することが岡畑ら、Ｊ．ＣＨＥＭ．ＳＯＣ．，ＣＨＥＭ．ＣＯＭＭＵＮ．１９９２，１３３９−１３４１および特開平８−２３９３９８号公報に発表されている。発表されたＤＮＡ塩の両親媒性カチオンは一般に構造が複雑で、その合成に手間がかかる。そこでもっと容易に合成することができるか、又は市場において容易に入手し得る両親媒性分子を使用して製造することができる機能性ＤＮＡ塩を提供することが望まれる。 DNA is a macromolecule having a phosphate anion. Okabata et al., J., et al., Produced an amphiphilic DNA salt by ion-bonding a cationic amphiphilic molecule to this phosphate anion. CHEM. SOC. , CHEM. COMMUN. 1992, 1339-1341 and JP-A-8-239398. The amphipathic cation of a DNA salt that has been announced generally has a complicated structure and takes time to synthesize. It is therefore desirable to provide functional DNA salts that can be synthesized more easily or can be produced using amphiphilic molecules that are readily available on the market.
本発明は、ＤＮＡリン酸アニオンが、
式Ｉ：
を有するカチオンとイオン結合してなるＤＮＡ塩を提供する。式Ｉ中、RはＣ８〜Ｃ２０アルキル、Ｃ８〜Ｃ２０アルキル置換基を有するベンジル、またはＣ８〜Ｃ２０アルキル置換基を有するフェノキシエトキシエチルから選ばれ、Ｒ’，Ｒ’’およびＲ’’’の２つはＣ１〜Ｃ４アルキルであり、残りの２つの両方がＣ１〜Ｃ４アルキルか、一方がＣ１〜Ｃ４アルキルで他方がベンジルか、または一方がベンジルで他方がフェニルであり、あるいはＲ’，Ｒ’’およびＲ’’’のそれらが結合するN原子と共にピリジン環を表す。
In the present invention, the DNA phosphate anion is
Formula I:
Provided is a DNA salt formed by ion bonding with a cation having In formula I, R is selected from phenoxyethoxyethyl having benzyl, or a C 8 -C 20 alkyl substituents having C 8 -C 20 alkyl, C 8 -C 20 alkyl substituent, R ', R''and Two of R ′ ″ are C 1 -C 4 alkyl, and the other two are both C 1 -C 4 alkyl, one is C 1 -C 4 alkyl and the other is benzyl, or one is benzyl The other is phenyl or represents a pyridine ring with the N atom to which they of R ′, R ″ and R ′ ″ are attached.
本発明は、上記ＤＮＡ塩を成膜して得られる機能性高分子膜を提供する。この膜は補強用高分子マトリックスを含むことができる。膜は、例えばＯ２ガスに対して高い選択的透過率を有し、医療用途において有用である。また膜を構成する２本鎖ＤＮＡ間に多種類の分子をインターカレーションすることにより、多種類の用途において有用である。 The present invention provides a functional polymer film obtained by forming the DNA salt into a film. The membrane can include a reinforcing polymer matrix. The membrane has a high selective permeability for eg O 2 gas and is useful in medical applications. Moreover, it is useful in many kinds of applications by intercalating many kinds of molecules between the double-stranded DNAs constituting the membrane.
ＤＮＡは、天然および合成ＤＮＡのいずれでも良いが、両親媒性カチオンとの塩を生成させるため、水溶性のＤＮＡ塩、例えばナトリウム塩を使用する。 The DNA may be either natural or synthetic DNA, but a water-soluble DNA salt such as a sodium salt is used to form a salt with an amphiphilic cation.
式Ｉの４級アンモニウムおよびピリジニウムカチオンは、クロライドまたはブロマイドの形で市場において容易に入手することができる。これらは逆性石鹸として知られる殺菌剤である。もし市場において入手できないときは、例えばＣ８〜Ｃ２０アルキルアミンやＣ１〜Ｃ４アルキルハライドを用いて４級化するか、またはピリジンをＣ８〜Ｃ２０アルキルハライドで４級化すること等によって容易に合成することができる。本発明において好適に使用し得る４級アンモニウム塩またはピリジニウム塩は、例えば
Ｃ８〜Ｃ２０アルキルトリメチルアンモニウムクロライド、
Ｃ８〜Ｃ２０アルキルジメチルエチルアンモニウムクロライド、
メチルＣ８〜Ｃ２０アルキルベンジルトリメチルアンモニウムクロライド、
Ｃ８〜Ｃ２０アルキルジメチルベンジルアンモニウムクロライド（ベンズアルコニウムクロライド）、
ジメチルフェニルベンジルアンモニウムクロライド、
Ｃ８〜Ｃ２０アルキルジメチル−３，４−ジクロロベンジルアンモニウムクロライド
４−（１，１，３，３−テトラメチルブチル）フェノキシエトキシエチルジメチルアンモニウムクロライド（ベンゼトニウムクロライド）
４−（１，１，３，３−テトラメチルブチル）クレゾキシエトキシエチル
ジメチルアンモニウムクロライド（メチルベンゼトニウムクロライド）、
Ｃ８〜Ｃ２０アルキルビリジニウムクロライド
およびこれらの対応するブロマイドを含む。
The quaternary ammonium and pyridinium cations of formula I are readily available on the market in the form of chloride or bromide. These are bactericides known as inverse soaps. If time is not available in the market, for example C 8 -C 20 alkylamine or C 1 -C 4 or quaternized with an alkyl halide, or pyridine C 8 -C 20 alkyl halide in the quaternization can be like Can be easily synthesized. Examples of the quaternary ammonium salt or pyridinium salt that can be preferably used in the present invention include C 8 to C 20 alkyltrimethylammonium chloride,
C 8 -C 20 alkyl dimethyl ethyl ammonium chloride,
Methyl C 8 -C 20 alkyl benzyl trimethyl ammonium chloride,
C 8 -C 20 alkyl dimethyl benzyl ammonium chloride (benzalkonium chloride),
Dimethylphenylbenzylammonium chloride,
C 8 -C 20 alkyl dimethyl 3,4-dichlorobenzyl ammonium chloride 4- (1,1,3,3-tetramethylbutyl) phenoxy ethoxyethyl dimethyl ammonium chloride (benzethonium chloride)
4- (1,1,3,3-tetramethylbutyl) cresoxyethoxyethyldimethylammonium chloride (methylbenzethonium chloride),
C 8 -C including 20 alkyl pyridinium chloride and their corresponding bromides.
本発明のＤＮＡ塩は、水溶液中において、ナトリウム塩のような水溶性ＤＮＡ塩と、該ＤＮＡに対し少なくとも当量の前記アンモニウム塩またはピリジニウム塩を反応させることによって得られる。この際アンモニウム塩またはピリジニウム塩水溶液中に、攪拌下ＤＮＡ塩水溶液を徐々に滴下するのが好ましい。生成する本発明のＤＮＡ塩は水に不溶なため白色沈澱として析出する。これを吸引濾過して集め、精製水でよく洗った後減圧乾燥することにより目的物が得られる。この塩はＤＮＡ２本鎖構造を保持している。 The DNA salt of the present invention is obtained by reacting a water-soluble DNA salt such as a sodium salt with at least an equivalent amount of the ammonium salt or pyridinium salt with respect to the DNA in an aqueous solution. At this time, the aqueous DNA salt solution is preferably gradually dropped into the aqueous ammonium salt or pyridinium salt solution with stirring. The resulting DNA salt of the present invention is insoluble in water and thus precipitates as a white precipitate. This is collected by suction filtration, washed well with purified water, and then dried under reduced pressure to obtain the desired product. This salt retains the DNA double-stranded structure.
本発明のＤＮＡ塩はクロロホルム／エタノール混液のような有機溶媒に可溶である。そのためこの溶液をガラス板のような基板にキャストし、乾燥して成膜することができる。膜の用途によって機械的強度が要求される場合には補強材と複合化することができる。そのような補強材を含む補強膜を作製する第１の方法は、布、不織布、多孔質フィルムのような補強支持材に溶液を塗布し、乾燥して異方性膜を作製する方法である。塗布の代りに補強支持材をＤＮＡ塩溶液で含浸してもよい。第２の方法は、ＤＮＡ塩と補強用高分子を両者の共通有機溶媒に溶かし、ガラス板の上に溶液をキャストし、乾燥後剥離する方法である。例えば補強用高分子がポリアミドイミドの場合、溶媒としてジメチルホルムアミド（ＤＭＦ）を用いることができる。 The DNA salt of the present invention is soluble in an organic solvent such as a chloroform / ethanol mixture. Therefore, this solution can be cast on a substrate such as a glass plate and dried to form a film. When mechanical strength is required depending on the use of the membrane, it can be combined with a reinforcing material. A first method for producing a reinforcing membrane containing such a reinforcing material is a method of producing an anisotropic membrane by applying a solution to a reinforcing support material such as a cloth, a nonwoven fabric, or a porous film and drying it. . Instead of coating, the reinforcing support may be impregnated with a DNA salt solution. The second method is a method in which a DNA salt and a reinforcing polymer are dissolved in a common organic solvent of both, a solution is cast on a glass plate, and dried and peeled off. For example, when the reinforcing polymer is polyamideimide, dimethylformamide (DMF) can be used as a solvent.
このようにして得られた本発明のＤＮＡ塩膜は、Ｏ２ガスに対して選択的に高い透過率を有することがわかった。このため膜は気体分離膜として、または高いＯ２透過性を要求する医療用途において有用である。また、成膜前のＤＮＡ塩に機能性分子をインターカレーションすることにより、インターカレーションした分子の性質を利用する機能性高分子膜として有用である。 It was found that the thus obtained DNA salt film of the present invention has a selectively high transmittance with respect to O 2 gas. Thus, the membrane is useful as a gas separation membrane or in medical applications that require high O 2 permeability. In addition, it is useful as a functional polymer film that utilizes the properties of intercalated molecules by intercalating functional molecules with DNA salts before film formation.
以下の実施例は本発明を例証する目的で与えられ限定を意図しない。なお、すべての実施例においてＤＮＡとしてサケ精子ＤＮＡナトリウム塩（日本化学飼料社製）を用いた。 The following examples are given for the purpose of illustrating the invention and are not intended to be limiting. In all examples, salmon sperm DNA sodium salt (manufactured by Nippon Chemical Feed Co., Ltd.) was used as DNA.
実施例１ ＤＮＡ／ＣＴＭＡの合成
２Ｌビーカーに超純水１１００ｍｌを入れ、これにＤＮＡ−Ｎａ ４ｇ（４．５７×１０−３ｍｏｌ）を加え、マグネチックスターラーで攪拌して溶解した。別の２Ｌビーカーに超純水１１００ｍｌを入れ、セチルトリメチルアンモニウムクロライド（ＣＴＭＡＣｌ）３．４２ｇ（１．０７×１０−２ｍｏｌ）を加えて溶解した。ＤＮＡ−Ｎａ溶液を滴下ロートに移し、ＣＴＭＡＣｌ溶液中に攪拌下ゆっくり滴下した。滴下するとすぐ白色の沈澱が生成した。滴下終了後３時間攪拌を継続し、反応液を冷蔵庫で１２時間静置した。その後白色沈澱物を吸引濾過し、超純水で２〜３回洗浄し、シャーレに生成物を移し、４０℃で１日減圧乾燥した。収率９４％であった。
Example 1 Synthesis of DNA / CTMA 1100 ml of ultrapure water was placed in a 2 L beaker, 4 g (4.57 × 10 −3 mol) of DNA-Na was added thereto, and the mixture was dissolved by stirring with a magnetic stirrer. In another 2 L beaker, 1100 ml of ultrapure water was added, and 3.42 g (1.07 × 10 −2 mol) of cetyltrimethylammonium chloride (CTMACl) was added and dissolved. The DNA-Na solution was transferred to a dropping funnel and slowly dropped into the CTMACl solution with stirring. A white precipitate formed immediately after the addition. Stirring was continued for 3 hours after completion of dropping, and the reaction solution was allowed to stand in a refrigerator for 12 hours. Thereafter, the white precipitate was filtered by suction, washed with ultrapure water 2-3 times, transferred to a petri dish, and dried under reduced pressure at 40 ° C. for 1 day. The yield was 94%.
実施例２ ＤＮＡ／ＣＴＭＡ膜の製造
実施例１で得たＤＮＡ／ＣＴＭＡ０．３ｇをクロロホルム：エタノール（４：１）２２ｇに溶解し、これをガラス板上にキャストし、４０℃で３０分減圧乾燥し、フィルムを得た。
Example 2 Production of DNA / CTMA membrane 0.3 g of DNA / CTMA obtained in Example 1 was dissolved in 22 g of chloroform: ethanol (4: 1), cast on a glass plate, and dried under reduced pressure at 40 ° C. for 30 minutes. And a film was obtained.
実施例３ ＤＮＡ／ＤＴＭＡの合成
ＣＴＭＡＣｌ ３．４２ｇに代えて、ドデシルトリメチルアンモニウムクロライド（ＤＴＭＡＣｌ）２．８ｇ（１．０７×１０−２ｍｏｌ）を用いること以外は実施例１と同様にしてＤＮＡ／ＤＴＭＡを得た。収率は９６％であった。
Example 3 Synthesis of DNA / DTMA DNA / DTMA was synthesized in the same manner as in Example 1 except that 2.8 g (1.07 × 10 −2 mol) of dodecyltrimethylammonium chloride (DTMACl) was used instead of 3.42 g of CTMAC1. DTMA was obtained. The yield was 96%.
実施例４ ＤＮＡ／ＤＴＭＡ膜の製造
ＤＮＡ／ＣＴＭＡの代りに実施例３で得たＤＮＡ／ＤＴＭＡを使用する以外は実施例２と同様にしてＤＮＡ／ＤＴＭＡ膜を製造した。
Example 4 Production of DNA / DTMA Membrane A DNA / DTMA membrane was produced in the same manner as in Example 2 except that DNA / DTMA obtained in Example 3 was used instead of DNA / CTMA.
実施例５ ＤＮＡ／ＴＤＴＭＡの合成
ＣＴＭＡＣｌ ３．４２ｇに代えて、テトラデシルトリメチルアンモニウムクロライド（ＴＤＴＭＡＣｌ）３．５９ｇ（１．０７×１０−２ｍｏｌ）を用いること以外は実施例１に同様にしてＤＮＡ／ＴＤＴＭＡを得た。収率は９４．５％であった。
Example 5 Synthesis of DNA / TDTMA DNA was replaced in the same manner as in Example 1 except that 3.59 g (1.07 × 10 −2 mol) of tetradecyltrimethylammonium chloride (TDTMACl) was used instead of 3.42 g of CTMAC1. / TDTMA was obtained. The yield was 94.5%.
実施例６ ＤＮＡ／ＴＤＴＭＡ膜の製造
ＤＮＡ／ＣＴＭＡの代りに実施例５で得たＤＮＡ／ＴＤＴＭＡを使用する以外は実施例２と同様にしてＤＮＡ／ＴＤＴＭＡ膜を製造した。
Example 6 Production of DNA / TDTMA Membrane A DNA / TDTMA membrane was produced in the same manner as in Example 2, except that DNA / TDTMA obtained in Example 5 was used instead of DNA / CTMA.
実施例７ ＤＮＡ／ＳＴＭＡの合成
ＣＴＭＡＣｌ ３．４２ｇに代えて、ステアリルトリメチルアンモニウムクロライド（ＳＴＭＡＣｌ）３．７０ｇ（１．０７×１０−２ｍｏｌ）を用いること以外は実施例１と同様にしてＤＮＡ／ＳＴＭＡを得た。収率９３％であった。
Example 7 Synthesis of DNA / STMA DNA / STMA was replaced with DNA / STMA in the same manner as in Example 1 except that 3.70 g (1.07 × 10 −2 mol) of stearyltrimethylammonium chloride (STMACl) was used instead of 3.42 g. Obtained STMA. The yield was 93%.
実施例８ ＤＮＡ／ＳＴＭＡ膜の製造
ＤＮＡ／ＣＴＭＡの代りに実施例７で得たＤＮＡ／ＳＴＭＡを使用する以外は実施例２と同様にしてＤＮＡ／ＳＴＭＡ膜を製造した。
Example 8 Production of DNA / STMA Membrane A DNA / STMA membrane was produced in the same manner as in Example 2 except that DNA / STMA obtained in Example 7 was used instead of DNA / CTMA.
実施例９ ＤＮＡ／ＢＤＭＴＤＡの合成
ＣＴＭＡＣｌ ３．４２ｇに代えて、ベンジルジメチルテトラデシルアンモニウムクロライド（ＢＤＭＴＤＡＣｌ）３．９３ｇ（１．０７×１０−２ｍｏｌ）を用いる以外は実施例１と同様にしてＤＮＡ／ＢＤＭＴＤＡを得た。収率９６％であった。
Example 9 Synthesis of DNA / BDMTDA DNA was replaced in the same manner as in Example 1 except that 3.93 g (1.07 × 10 −2 mol) of benzyldimethyltetradecylammonium chloride (BDMTDACl) was used instead of 3.42 g of CTMAC1. / BDMTDA was obtained. The yield was 96%.
実施例１０ ＤＮＡ／ＢＤＭＴＤＡ膜の製造
ＤＮＡ／ＣＴＭＡの代りに実施例９で得たＤＮＡ／ＢＤＭＴＤＡを使用する以外は実施例２と同様にしてＤＮＡ／ＢＤＭＴＤＡ膜を製造した。
Example 10 Production of DNA / BDMTDA Membrane A DNA / BDMTDA membrane was produced in the same manner as in Example 2 except that DNA / BDMTDA obtained in Example 9 was used instead of DNA / CTMA.
実施例１１ ＤＮＡ／ＢＣＤＭＡの合成
ＣＴＭＡＣｌ ３．４ｇに代えて、ベンジルセチルジメチルアンモニウムクロライド（ＢＣＤＭＡＣｌ）４．２３ｇ（１．０７×１０−２ｍｏｌ）を用いる以外は実施例１と同様にしてＤＮＡ／ＢＣＤＭＡを得た。収率９４％であった。
Example 11 Synthesis of DNA / BCDMA DNA / BCDMA was prepared in the same manner as in Example 1 except that 4.23 g (1.07 × 10 −2 mol) of benzylcetyldimethylammonium chloride (BCDMACl) was used instead of 3.4 g of CTMAC1. BCDMA was obtained. The yield was 94%.
実施例１２ ＤＮＡ／ＢＣＤＭＡ膜の製造
ＤＮＡ／ＣＴＭＡの代りに実施例１１で得たＤＮＡ／ＢＣＤＭＡを使用する以外は実施例２と同様にしてＤＮＡ／ＢＣＤＭＡ膜を製造した。
Example 12 Production of DNA / BCDMA Membrane A DNA / BCDMA membrane was produced in the same manner as in Example 2 except that DNA / BCDMA obtained in Example 11 was used instead of DNA / CTMA.
実施例１３ ＤＮＡ／ＢＤＭＳＡの合成
ＣＴＭＡＣｌ ３．４ｇに代えて、ベンジルジメチルステアリルアンモニウムクロライド（ＢＤＭＳＡＣｌ）４．５ｇ（１．０７×１０−２ｍｏｌ）を用いる以外は実施例１と同様にしてＤＮＡ／ＢＤＭＳＡを得た。収率９６．５％であった。
Example 13 Synthesis of DNA / BDMSA DNA / BDMSA was replaced in the same manner as in Example 1 except that 4.5 g (1.07 × 10 -2 mol) of benzyldimethylstearylammonium chloride (BDMSACl) was used instead of 3.4 g. BDMSA was obtained. The yield was 96.5%.
実施例１４ ＤＮＡ／ＢＤＭＳＡの製造
ＤＮＡ／ＣＴＭＡの代りに、実施例１３で得たＤＮＡ／ＢＤＭＳＡを使用する以外は実施例２と同様にしてＤＮＡ／ＢＤＭＳＡ膜を製造した。
Example 14 Production of DNA / BDMSA A DNA / BDMSA membrane was produced in the same manner as in Example 2 except that DNA / BDMSA obtained in Example 13 was used instead of DNA / CTMA.
実施例１５ ＤＮＡ／ＣＰの合成
ＣＴＭＡＣｌ ３．４ｇに代えて、セチルピリジニウムクロライド（ＣＰＣｌ）３．６ｇ（１．０７×１０−２ｍｏｌ）を用いる以外は実施例１と同様にしてＤＮＡ／ＣＰを得た。収率は殆んど定量的であった。
Example 15 Synthesis of DNA / CP DNA / CP was prepared in the same manner as in Example 1 except that 3.6 g (1.07 × 10 −2 mol) of cetylpyridinium chloride (CPCl) was used instead of 3.4 g of CTMAC1. Obtained. The yield was almost quantitative.
実施例１６ ＤＮＡ／ＣＰ膜の製造
ＤＮＡ／ＣＴＭＡの代りに、実施例１５で得たＤＮＡ／ＣＰを使用する以外は実施例２と同様にしてＤＮＡ／ＣＰ膜を製造した。
Example 16 Production of DNA / CP Membrane A DNA / CP membrane was produced in the same manner as in Example 2 except that DNA / CP obtained in Example 15 was used instead of DNA / CTMA.
実施例１７ ＤＮＡ／ベンゼトニウム塩の合成
ＣＴＭＡＣｌ ３．４ｇに代えて、塩化ベンゼトニウム（４−（１，１，３，３−テトラメチルブチル）フェノキシエトキシエチルジメチルアンニウムクロライド）４．８ｇ（１．０７×１０−２ｍｏｌ）を用いる以外は実施例１と同様にしてＤＮＡ／ベンゼトニウム塩を得た。収率は殆んど定量的であった。
Example 17 Synthesis of DNA / Benzetonium Salt Instead of CTMACl 3.4 g, 4.8 g (1.07) of benzethonium chloride (4- (1,1,3,3-tetramethylbutyl) phenoxyethoxyethyldimethylanium chloride) A DNA / benzethonium salt was obtained in the same manner as in Example 1 except that × 10 −2 mol) was used. The yield was almost quantitative.
実施例１８ 多孔質ＰＴＦＥ補強ＤＮＡ／ＣＰ膜
実施例１６で合成したＤＮＡ／ＣＰ ０．３ｇをクロロホルム：エタノール（４：１）２２ｇに溶解し、この溶液でポリテトラフルオロエチレン多孔質フィルムを含浸し、４０℃で１日乾燥して補強ＤＮＡ／ＣＰ膜を製造した。
Example 18 Porous PTFE-reinforced DNA / CP membrane 0.3 g of DNA / CP synthesized in Example 16 was dissolved in 22 g of chloroform: ethanol (4: 1), and this solution was impregnated with a polytetrafluoroethylene porous film. The reinforced DNA / CP membrane was produced by drying at 40 ° C. for 1 day.
実施例１９ ポリアミドイミドブレンドＤＮＡ／ＣＴＭＡ膜
実施例１で合成したＤＮＡ／ＣＴＭＡと、ポリアミドイミド（Ｓｏｌｖａｙ Ａｄｖａｎｃｅｄ Ｐｏｌｙｍｅｒ社製ＴＯＲＬＯＮ）とを１対１の割合でＤＭＦに溶解し、ガラス板上にキャストし、１５０℃で乾燥後剥離してポリアミドイミドブレンドＤＮＡ／ＣＴＭＡ膜を製造した。
Example 19 Polyamideimide blend DNA / CTMA membrane DNA / CTMA synthesized in Example 1 and polyamideimide (TORLON manufactured by Solvay Advanced Polymer) were dissolved in DMF at a ratio of 1: 1 and cast on a glass plate. After drying at 150 ° C., peeling was performed to produce a polyamideimide blend DNA / CTMA film.
ガス透過性試験
実施例で製造した膜のいくつかについて、ＪＩＳ Ｋ７１２６１９８７に準じてＯ２およびＮ２について気体透過率を測定した。ツクバリカセイキ（株）製気体透過率測定装置を使用し、温度および供給（上流側）圧力条件は、それぞれ２５℃および１０６ｋＰａに設定した。またＯ２およびＮ２の透過係数からＰ（Ｏ２）／Ｐ（Ｎ２）の比を求め、他のガスに対するＯ２ガス選択透過性の指標とした。結果を表１に示す。
For some of the membranes produced in the gas permeability test example, gas permeability was measured for O 2 and N 2 according to JIS K71261987. Using a gas permeability measuring device manufactured by Tsukubarika Seiki Co., Ltd., temperature and supply (upstream side) pressure conditions were set to 25 ° C. and 106 kPa, respectively. The calculated ratio of P (O 2) / P ( N 2) from the transmission coefficient of O 2 and N 2, was O 2 gas permselective indicator for other gases. The results are shown in Table 1.
水蒸気透過性試験
実施例で製造したいくつかについて水蒸気透過率を測定した。密閉可能なガラス製サンプルホルダーにＤＮＡ脂質膜を装着し、サンプルホルダー内に入れた水を５５℃に加温し、一定時間内に透過した水蒸気量をサンプルホルダー内に残存した水重量から算出した。なお、水温５５℃の飽和水蒸気圧は約１５７ｈＰａである。結果を表２に示す。
Water vapor permeability test The water vapor permeability was measured for some of the examples produced in the examples. A DNA lipid membrane was attached to a sealable glass sample holder, the water in the sample holder was heated to 55 ° C., and the amount of water vapor permeated within a certain time was calculated from the weight of water remaining in the sample holder. . The saturated water vapor pressure at a water temperature of 55 ° C. is about 157 hPa. The results are shown in Table 2.
上で実施した水蒸気透過試験方法を採用して汎用的なフィルムの水蒸気透過性試験を実施した。汎用的なフィルムと比較すると実施例で製造した膜は水蒸気透過率が極めて高い特徴を示した。結果を表３に示す。 A water vapor permeability test of a general-purpose film was performed by adopting the water vapor permeability test method performed above. Compared with a general-purpose film, the membrane produced in the example showed the feature of extremely high water vapor permeability. The results are shown in Table 3.
Claims (3)
The functional polymer membrane according to claim 2, comprising a reinforcing polymer matrix.
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| JP2007091647A (en) * | 2005-09-29 | 2007-04-12 | Trekion Co Ltd | Antimicrobial dna salt composition |
| JP2009084225A (en) * | 2007-09-28 | 2009-04-23 | Dainippon Printing Co Ltd | Porous material, cell culture membrane, method for producing porous material and method for producing cell culture membrane |
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| JPH08239398A (en) * | 1995-03-03 | 1996-09-17 | Sogo Yatsukou Kk | Water-insoluble, self-supporting transparent dna/lipid complex film and its production |
| JP2001294597A (en) * | 2000-04-12 | 2001-10-23 | Asahi Denka Kogyo Kk | Complex |
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| JPH08239398A (en) * | 1995-03-03 | 1996-09-17 | Sogo Yatsukou Kk | Water-insoluble, self-supporting transparent dna/lipid complex film and its production |
| JP2001294597A (en) * | 2000-04-12 | 2001-10-23 | Asahi Denka Kogyo Kk | Complex |
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| JP2007091647A (en) * | 2005-09-29 | 2007-04-12 | Trekion Co Ltd | Antimicrobial dna salt composition |
| JP2009084225A (en) * | 2007-09-28 | 2009-04-23 | Dainippon Printing Co Ltd | Porous material, cell culture membrane, method for producing porous material and method for producing cell culture membrane |
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