JP2006340668A - Microorganism culture for decomposing styrene-acrylonitrile resin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microorganism culture that stably can decompose styrene-acrylonitrile resin with high efficiency and facilitates the decomposition of the resin substantially in no accompany with CO<SB>2</SB>occurrence and can recycle unused useful metals and useful compounds in the waste matter including the resin. <P>SOLUTION: The microorganism culture includes a microorganism in Pseudomonas boreopolis and can at least biodegrade styrene-acrylonitrile compound. The composition for decomposing styrene-acrylonitrile compound includes the microorganism culture. In the method for decomposing styrene-acrylonitrile, a product or waste including styrene-acrylonitrile compound is allowed to contact with the microorganism culture. Waste matter including styrene-acrylonitrile compound, metals or pigments is allowed to contact with the microorganism culture or a decomposition composition to decompose the styrene-acrylonitrile compound in the waste matter to separate the metal and pigments in the waste matter thereby recovering the metals and pigments from the waste matter. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a microorganism culture exhibiting a biodegradability of styrene acrylic resin, a composition for decomposing the resin, and a method for recovering metal or pigment in waste.

Conventionally, waste containing resin, such as toner, is treated by incineration in an incinerator or the like. However, when processing by the incineration is performed, there is a drawback that it may be difficult to recover useful resources contained in the waste. In addition, the treatment by incineration requires petroleum resources and the like, and has the disadvantage that it involves generation of exhaust gas such as CO ₂ .

On the other hand, it has been reported that a polyester resin that is easily degraded by a hydrolysis reaction can be biodegraded by a specific microorganism (for example, see Non-Patent Document 1). However, in the present situation, it is difficult for a microorganism capable of biodegrading a polyester resin to biodegrade a resin other than the polyester resin.
Yutaka Tokiwa, Sen'i Gakkaishi, Vol. 7, pp. 521 to 526 (1991)

The present invention, in one aspect, decomposes a resin, for example, a styrene acrylic resin with higher efficiency, more stably decomposes the resin, and substantially eliminates the generation of CO _2. Achieved at least one of decomposing, making it easier to recycle unused useful metals and useful compounds in waste containing the resin, and substantially assimilating the products generated by decomposition Another object of the present invention is to provide a microorganism culture exhibiting the biodegradability of styrene acrylic resin. Furthermore, the present invention, in another aspect, decomposes the resin with higher efficiency, decomposes the resin more stably, and decomposes the resin without substantially generating CO _2. And providing a composition for decomposing the styrene-acrylic resin, which can achieve at least one of recycling of useful metals, useful compounds, etc. that are unused in waste containing the resin. is there. In another aspect of the present invention, the resin can be efficiently decomposed, the resin can be stably decomposed, the resin can be decomposed at low cost, and the resin can be decomposed with low influence on the surrounding environment. Another object of the present invention is to provide a method for decomposing a styrene acrylic resin, which can achieve at least one of decomposing the resin substantially without generating CO ₂ . Still another aspect of the present invention is to facilitate recycling of unused useful metals and useful compounds in waste containing the resin, and to recover useful metals and useful compounds from the waste at low cost. Another object of the present invention is to provide a method for recovering metals or pigments in waste, which can achieve at least one of recovering useful metals, useful compounds, etc. in waste with a low influence on the surrounding environment. .

That is, the gist of the present invention is as follows.
[1] A microorganism culture exhibiting a biodegradability of styrene acrylic resin, comprising microorganisms belonging to Pseudomonas boreeopolis,
[2] The microorganism culture according to [1] above, wherein the microorganism belonging to Pseudomonas boreopolis is Pseudomonas boreopolis Q-5 (accession number: NITE P-80),
[3] A microorganism belonging to Luteimonas sp., A microorganism belonging to Mesorhizobium loti, a microorganism belonging to Rocharimaea elizabethee, and a microorganism belonging to Alpenia sp. The microorganism culture according to [1] or [2], further comprising:
[4] A composition for decomposing a styrene acrylic resin, comprising the microorganism culture according to any one of [1] to [3],
[5] The composition for decomposing a styrene acrylic resin according to the above [4], further comprising a fixing carrier suitable for holding a microorganism culture.
[6] The composition for decomposing a styrene acrylic resin according to the above [4] or [5], further comprising a medium suitable for culturing microorganisms,
[7] (A) A product or waste containing a styrene acrylic resin, and (B) the microorganism culture according to any one of [1] to [3] or any of [4] to [6] (8) (a) waste containing styrene acrylic resin and a metal or a pigment, and (b) A styrene acrylic resin in waste by contacting the microorganism culture according to any one of [1] to [3] or the decomposition composition according to any one of [4] to [6]. A method for recovering metal or pigment in waste, characterized in that the metal or pigment in waste is separated thereby
About.

According to the microorganism culture of the present invention, a resin, in particular, a styrene acrylic resin, can be decomposed more stably with higher efficiency without substantially generating CO ₂ , and generated by the decomposition. The product can be substantially assimilated, and has an excellent effect that it is possible to more easily recycle unused useful metals and useful compounds in wastes including resins, particularly styrene acrylic resins. . According to the composition for decomposing a styrene acrylic resin of the present invention, a resin, in particular, a styrene acrylic resin can be decomposed more stably with higher efficiency without substantially generating CO _2. In addition, there is an excellent effect that recycling of unused useful metals, useful compounds, etc. in wastes containing resins, particularly styrene acrylic resins, can be facilitated. Further, according to the method for decomposing a styrene acrylic resin of the present invention, a resin, particularly a styrene acrylic resin, can be stably decomposed with high efficiency and substantially without generation of CO ₂ , An excellent effect that recycling of unused useful metals, useful compounds, etc. in wastes containing resins, particularly styrene acrylic resins, can be facilitated. Furthermore, according to the method for recovering a metal or pigment in waste of the present invention, it is possible to more easily recycle unused useful metals and useful compounds in the waste containing the resin, and the useful metals, useful compounds and the like. Can be recovered at low cost with low impact on the surrounding environment.

  One aspect of the present invention is a microbial culture that exhibits at least the biodegradability of a styrene acrylic resin, containing microorganisms belonging to Pseudomonas boreopolis.

  In the present specification, “at least exhibiting the biodegradability of styrene acrylic resin” means that the biodegradability of styrene acrylic resin is detected by the degradation activity evaluation method described later. It is intended not to be limited by the presence or absence of activity. Therefore, in another aspect, the microbial culture of the present invention includes those that further exhibit degradation activity against chemically synthesized resins such as polyester resins and styrene acrylic resins.

  Since the microorganism culture of the present invention contains the microorganism, it has an excellent effect of being able to efficiently and stably biodegrade styrene acrylic resin, which has heretofore been difficult to biodegrade. To do. Therefore, according to the microorganism culture of the present invention, it is possible to biodegrade styrene acrylic resin, which is a hardly decomposable resin used in toners for copying machines and printers.

In addition, since the microorganism culture of the present invention contains the microorganism, the generation of CO ₂ based on the decomposition of the resin is substantially suppressed. Therefore, according to the microorganism culture of the present invention, when processing waste containing a resin, particularly a styrene acrylic resin, it is possible to substantially suppress the release of CO ₂ into the atmosphere. Excellent effect in terms of.

  Furthermore, according to the microorganism culture of the present invention, a resin, particularly a resin in waste containing a styrene acrylic resin, in particular, a styrene acrylic resin can be decomposed, and an unused useful metal in the waste. Alternatively, it is possible to facilitate the collection of useful dyes. Therefore, according to the microorganism culture of the present invention, it is possible to make it easier to recycle unused useful metals or useful pigments in wastes, and thus excellent effects in terms of effective use of resources, environmental protection, and the like. Demonstrate.

  Moreover, the microorganism culture of the present invention exhibits an excellent property that it can substantially assimilate the product produced by the degradation. Therefore, accumulation of products generated by the decomposition is substantially suppressed, and thus an excellent effect in terms of environmental protection and the like is exhibited.

  In this specification, the waste refers to a waste containing a resin, in particular, a styrene acrylic resin. Specifically, for example, a toner, a can laminate, an insulator for electronic devices, and a waste for home appliances. , Automobile waste, industrial waste, and the like.

  Examples of the styrene acrylic resin include compounds obtained by polycondensation of acrylic acid compounds such as acrylic acid n-butyl ester and styrene. Specifically, the styrene acrylic resin is not particularly limited, and examples thereof include acrylonitrile acrylate styrene and styrene butyl acrylate. The average molecular weight, degree of polymerization, etc. of the styrene acrylic resin may be in a range that exhibits biodegradation (degradation activity of styrene acrylic resin) by the microorganism culture of the present invention.

In addition, as an evaluation method of the degradation activity of styrene acrylic resin by the microorganism culture of the present invention, for example,
1) FY medium containing styrene acrylic resin having a final concentration of 5 g / L [Composition: 1 wt% NH ₄ Cl, 0.1 wt% KH ₂ PO ₄ , 0.05 wt% K ₂ HPO ₄ , 0.05 wt % MgSO ₄ .7H ₂ O, 0.2 wt% NaCl, 0.001 wt% ZnCl ₂ , 0.001 wt% FeSO ₄ .7H ₂ O, 0.001 wt% CaCl ₂ , 0.1 wt% Yeast extract ] Inoculating the microorganism culture and culturing at 37 ° C. for 7 days with shaking at 125 strokes / minute,
2) A step of extracting the resin component remaining in the obtained culture broth with dichloromethane, adding hexatriacontane, and drying under reduced pressure with an evaporator;
3) The obtained dried product was dissolved in 5 mL of n-hexane: toluene / 2: 8 (volume ratio), and 1 μL of the obtained sample was treated with, for example, trade name: Chromarod SIII (0.1 × 10 cm) Steps subjected to thin-layer chromatography using (Mitsubishi Chemical Yatron Co., Ltd.) and developing with n-hexane, and 4) For example, trade name: Iatroscan Mk-6s (Mitsubishi Chemical Yatron Co., Ltd.) is used. Detector: FID, hydrogen gas: 160 mL / min, air: 2.0 L / min, scan speed: 30 sec / scan, corresponding to styrene acrylic resin and degradation products of styrene acrylic resin in the culture solution Detecting a peak to be detected,
The method including

  In the above step 1), in the FY medium containing the styrene acrylic resin, styrene acrylic resin dissolved in dichloromethane is dispensed into a test tube to a final concentration of 5 g / L, air-dried, and 5 mL of FY medium is added. It can be prepared by autoclaving at 121 ° C. for 20 minutes.

  In the above step 3), thin layer chromatography is carried out by incubating for 10 minutes in a humidity chamber (condition: 65% RH) and then developing at 37 ° C. in a developing tank containing n-hexane. sell.

  It should be noted that the evaluation of the degradation activity may be carried out in the same manner in the absence of a microorganism culture as a control. The degradation activity is evaluated by comparing the area of each peak in the presence of the microorganism culture with the area of each peak in the control. When the area of the peak corresponding to the styrene acrylic resin is zero, it becomes an indicator that the styrene acrylic resin was decomposed 100%, and the decomposition activity is [{(peak area corresponding to the control styrene acrylic resin) − ( The peak area corresponding to the styrene acrylic resin in the culture medium} / (peak area corresponding to the control styrene acrylic resin)] × 100 (%) is shown.

  In the evaluation of the decomposition activity of the styrene acrylic resin, the decomposition activity of the resin compound can be evaluated by using another resin compound dissolved in an appropriate solvent instead of the styrene acrylic resin.

  The microorganism belonging to Pseudomonas boreopolis is not particularly limited as long as it exhibits a degrading activity with respect to the styrene acrylic resin by the method for evaluating the degrading activity of the styrene acrylic resin. Specifically, Pseudomonas boreopolis Q-5 etc. are mentioned. The Pseudomonas boreopolis Q-5 is named and displayed as “Pseudomonas boreeopolis Q-5”, and under the accession number: NITE P-80 (contract date: February 28, 2005), an independent administrative agency product evaluation technology. Foundation Organization Deposited at the Patent Microorganism Depositary Center [Zip code 292-0818, Kisarazu City, Kazusa Kamashichi 2-5-8].

The Pseudomonas boreopolis Q-5 is 99.5% homologous to Pseudomonas boreopolis when analyzed by 16S rDNA based on the 16S rDNA library registered in DDBJ. . The Pseudomonas boreopolis Q-5 is a short gonococcus having gram-negative motility, and is characterized by colony: sticky, having oxidase, β-galactosidase, arginine dihydrolase, carboxylase Yes, ornithine decarboxylase Yes, urease Yes, tryptophan deaminase Yes, gelatinase Yes, use organic citric acid, Mu production of H ₂ S, Mu production of indole, Mu production of acetoin, Mu glucose assimilative, D- mannitol No assimilation, no inositol assimilation, no L-rhamnose assimilation, no D-sorbitol assimilation, no, no saccharose assimilation, no D-melibiose assimilation, no D-amygdalin assimilation -No arabinose utilization.

  The 16S rDNA analysis is based on the Blast (Basic Local Alignment Search Tool) algorithm (condition: search target division (bacteria, with GAP calculation, expected value 10) under default conditions, for example, DNA Data Bank of Japan ( DDBJ) data can be used to compare the target 16S rDNA base sequence with the known 16S rDNA base sequence.

  According to the Pseudomonas boreopolis Q-5, the biodegradation rate of about 40% as the biodegradation rate measured by the degradation activity of the styrene acrylic resin [supplied by Mikasa Sangyo Co., Ltd.] by the degradation activity evaluation method. Compared with the absence of the Pseudomonas boreopolis Q-5, for example, the biodegradation rate of about 2.0 mg / 5 mL / 7 days is excellent. Show properties.

  In the present invention, instead of the Pseudomonas boreopolis Q-5, it is a microorganism close to the evolutionary tree of the Pseudomonas boreopolis Q-5, wherein the degradation activity is evaluated. Depending on the method, a microorganism in which the degradation activity of the styrene acrylic resin is detected can also be used.

  The microbial culture of the present invention containing the Pseudomonas boreopolis Q-5 is not limited to any other product that can capture or assimilate the degradation products produced by the degradation of the styrene acrylic resin by the Pseudomonas boreopolis Q-5. It may further contain a microorganism. In another aspect, the microorganism culture of the present invention further contains another microorganism capable of degrading a resin, for example, a styrene acrylic resin, in cooperation with the Pseudomonas boreopolis Q-5. May be.

  The microorganism culture of the present invention is preferably a microorganism belonging to Luteimonas sp., A microorganism belonging to Mesorhizobium loti, and a microorganism belonging to Rocharimaea elisabeth ae. a microorganism culture further containing a microorganism belonging to sp.). According to such a microorganism culture, since the microorganisms can act cooperatively as a group, an excellent effect of maintaining higher biodegradability more stably with respect to the styrene acrylic resin is exhibited. Moreover, according to the microorganism culture of this invention, the said microorganisms can decompose | disassemble the said styrene acrylic resin cooperatively as a group, and can use it as a nutrient in each growth. Therefore, according to the microorganism culture of the present invention, the styrene acrylic resin can be decomposed with a low influence on the surrounding environment.

  More specifically, a microorganism culture of the mixed microorganism group AMOC2 can be mentioned.

  The AMOC2 is named and displayed as AMOC2, and is safely deposited with the National Institute of Technology and Evaluation (postal code 292-0818, 2-5-8, Kazusa Kamashichi, Kisarazu City, Chiba Prefecture). The AMOC2 is stored in the Kinki University Department of Agriculture, Department of Agricultural Chemistry, Applied Microbiology Laboratory (Postal Code: 631-8505, Nakamachi, Nara City, 3327-204). According to the handling related to the sale at the "Japan Patent Microorganism Deposit Center", the sale specified in Article 27-3 of the Patent Law Enforcement Regulations is made and available.

  According to the microorganism culture of AMOC2, the biodegradation rate of about 45% is achieved as the biodegradation rate measured by the degradation activity evaluation method of the styrene acrylic resin [supplied by Mikasa Sangyo Co., Ltd.]. Compared to the absence of the microbial culture, the biodegradation rate is at least about 2.3 mg / 5 mL / 7, which is an excellent property.

The AMOC2 is diluted in a sterilized physiological saline at an appropriate dilution rate, for example, R2A plate medium [composition: 0.05 wt% yeast extract, 0.05 wt% peptone, 0.05 wt% casamino acid, 0. 05 wt% glucose, 0.05 wt% soluble starch, 0.03 wt% K ₂ HPO ₄ , 0.005 wt% MgSO ₄ .7H ₂ O, 0.03 wt% sodium pyruvate, 1.5 wt% agar Applied to Difco Laboratories Co., Ltd., cultured at 37 ° C. for 7 to 14 days, and the resulting colonies were selected until they became homogeneous, so that Lutemonas sp. Q-1, Mesozobium Loti (Mesohizobium loti) Q-2, Q-3 strain, Q-4 strain, Pseudomona Boreopolis Q-5 [Pseudomonas boreeopolis Q-5 (Accession Number: NITE P-80, Accession Date: February 28, 2005)] and Alkaline Genes sp. Q-6 are confirmed to be contained It is a culture of mixed microorganisms.

  In the present invention, instead of the microorganisms contained in the AMOC2, the microorganisms are close to the evolutionary tree with the microorganisms, and the degradation activity of the styrene acrylic resin is detected by the degradation activity evaluation method. It is also possible to use microorganisms.

Here, the above-mentioned Luteimonas sp. Q-1 is based on the 16S rDNA library registered in DNA Data Bank of Japan (DDBJ) and has a homology with Luteimonas sp. TUT1238. 98.6%. In addition, the above-mentioned Luteimonas sp. Q-1 is a short gonococcus having gram-negative motility, colony characteristics: mucus, oxidase, β-galactosidase, arginine dihydrolase, lysine Mu decarboxylase, Mu ornithine decarboxylase, urease Mu, tryptophan deaminase Yes, gelatinase Yes, Mu use of citric acid, Mu production of H ₂ S, Mu production of indole, acetoin production Yes, Mu glucose assimilation, D- Mannitol assimilation, no inositol assimilation, no L-rhamnose assimilation, no D-sorbitol assimilation, no saccharose assimilation, no D-melibiose assimilation, no D-amygdalin assimilation, L-arabinose assimilation property None.

The Mesozobium loti Q-2 is based on the 16S rDNA library registered in DDBJ, and has a homology of 98.6% with the Mesozobium loti (Mesohizobium loti). Further, the Mesohizobium loti Q-2 is a short gonococcus having gram-negative motility, and a colony characteristic: red, oxidase present, β-galactosidase absent, arginine dihydrolase present, lysine decarboxylase No, Mu ornithine decarboxylase, urease Yes, tryptophan deaminase Yes, Mu gelatinase, Mu use of citric acid, Mu production of H ₂ S, Mu production of indole, acetoin production Yes, Mu glucose assimilative, D- mannitol No assimilation, no inositol assimilation, no L-rhamnose assimilation, no D-sorbitol assimilation, no saccharose assimilation, no D-melibiose assimilation, no D-amygdalin assimilation, L- No arabinose utilization.

Q-3 is based on a 16S rDNA library registered in DDBJ and has a homology of 96.5% with Rocharimaea elizabethee. Q-3 is a short gonococcus having gram-negative motility, colony characteristics: peripheral part is green, oxidase present, β-galactosidase absent, arginine dihydrolase present, lysine decarboxylase absent, ornithine Mu decarboxylase, Mu urease, tryptophan deaminase Yes, Mu gelatinase, use organic citric acid, Mu production of H ₂ S, Mu production of indole, acetoin production Yes, Mu glucose assimilative, D- mannitol assimilation Mu , Inositol assimilation, no L-rhamnose assimilation, no D-sorbitol assimilation, no saccharose assimilation, no D-melibiose assimilation, no D-amygdalin assimilation, no L-arabinose assimilation Sex is nothing.

Q-4 is based on the 16S rDNA library registered in DDBJ and has a homology of 96.6% with Rocharimaea elizabethae. Q-4 is a short gonococcus having gram-negative motility, and the characteristics of the colony are red at the periphery, oxidase present, β-galactosidase absent, arginine dihydrolase present, lysine decarboxylase absent, ornithine Mu decarboxylase, Mu urease, tryptophan deaminase Yes, Mu gelatinase, use organic citric acid, Mu production of H ₂ S, Mu production of indole, acetoin production Yes, Mu glucose assimilative, D- mannitol assimilation Mu , Inositol assimilation, no L-rhamnose assimilation, no D-sorbitol assimilation, no saccharose assimilation, no D-melibiose assimilation, no D-amygdalin assimilation, no L-arabinose assimilation Sex is nothing.

  The Pseudomonas boreopolis Q-5 is the same as described above.

The Alkaline genes sp. Q-6 is based on the 16S rDNA library registered in DDBJ, and has a homology of 99.6% with Alkaline genes sp. Mp-2. . Alkaligene sp. Q-6 is a short gonococcus having gram-negative motility, colony characteristics: colorless, oxidase present, β-galactosidase absent, arginine dihydrolase present, lysine Mu decarboxylase, ornithine decarboxylase Yes, urease Yes, tryptophan deaminase Yes, Mu gelatinase, use organic citric acid, Mu production of H ₂ S, Mu production of indole, acetoin production Yes, Mu glucose assimilation, D- Mannitol assimilation, no inositol assimilation, no L-rhamnose assimilation, no D-sorbitol assimilation, no saccharose assimilation, no D-melibiose assimilation, no D-amygdalin assimilation, L-arabinose assimilation property None.

The microorganism culture of the present invention contains the styrene acrylic resin or the like as a carbon source, and examples of the nitrogen source include NH ₄ Cl, (NH ₄ ) ₂ SO ₄ , NH ₄ NO ₃ , (NH ₄ ) ₂ CO. , (NH ₄ ) ₂ HPO ₄ , (NH ₄ ) H ₂ PO _{4 and the} like. The nitrogen source is preferably 0.5% by weight or more, more preferably 0.6% by weight or more, and further preferably 1.0% by weight from the viewpoint of supplying a sufficient nitrogen source for growing microorganisms. From the viewpoint of exerting sufficient growth ability for the microorganism, it is preferably 3.0% by weight or less, more preferably 1.7% by weight or less, and still more preferably 1.2% by weight or less. Is desirable. The carbon source / nitrogen source weight ratio (C / N ratio) is preferably 40 or more, more preferably 43 or more, and still more preferably 48 or more, from the viewpoint of adjusting the balance between the carbon source and the nitrogen source. From the viewpoint of exerting sufficient growth ability for the microorganism, it is preferably 60 or less, more preferably 55 or less, and still more preferably 50 or less. Further, the pH of the medium is preferably pH 6.5 to 7.5, and more preferably pH 6.9 to 7.1. The microorganism culture of the present invention is cultured under the above conditions, preferably at 30 to 40 ° C., more preferably at 35 to 37 ° C., for 100 to 150 strokes / minute, preferably 110 to 130 strokes / minute. Can be maintained.

Specifically, the microorganism culture of the present invention is prepared by using the FY medium, a styrene acrylic resin-added FY medium (composition: 0.5 wt% styrene acrylic resin, 1 wt% NH ₄ Cl, 0.1 wt% KH ₂ PO). ₄ , 0.05 wt% K ₂ HPO ₄ , 0.05 wt% MgSO ₄ .7H ₂ O, 0.2 wt% NaCl, 0.001 wt% ZnCl ₂ , 0.001 wt% FeSO ₄ .7H ₂ O 0.001 wt% CaCl ₂ , 0.1 wt% yeast extract) and the like.

  In another aspect, the present invention relates to a composition for decomposing a styrene acrylic resin.

The decomposition composition of the present invention contains the microorganism culture of the present invention. Therefore, according to the composition for decomposition of the present invention, a resin, in particular, a styrene acrylic resin can be decomposed more stably with higher efficiency without substantially generating CO _2. In particular, it is possible to make it easier to recycle unused useful metals, useful compounds, etc. in wastes containing styrene acrylic resin.

  The composition for decomposition of the present invention may further contain a fixing carrier suitable for holding a microbial culture from the viewpoint of stably holding the microbial culture for a long period of time.

  The fixing carrier is preferably a carrier that stably maintains the growth of each microorganism contained in the microorganism culture and does not inhibit the biodegradability. From the viewpoint of stably holding the microorganism culture over a long period of time. Preferably, the carrier is not substantially decomposed by the microorganism. Examples of the fixing carrier include a porous carrier, a nonwoven fabric, activated carbon, ceramics, and cellulose.

  Moreover, the composition for decomposition of the present invention may further contain a medium suitable for culturing microorganisms.

  In the composition for decomposition of the present invention, the microorganism culture of the present invention may be immobilized on the surface of the insoluble carrier, or may be immobilized or held inside. When the microorganism culture of the present invention is immobilized or held inside such a carrier, it is a carrier capable of giving and receiving various nutrients necessary for the growth of the microorganism and a resin to be decomposed, such as a styrene acrylic resin. Is desirable.

  The number of microbial cells contained in the decomposition composition of the present invention is not particularly limited, depending on the growth state of the microorganism, the degree of expression of biodegradability, the method of handling the decomposition composition, etc. It can be set appropriately.

  In another aspect, the present invention is characterized by contacting (A) a product or waste containing a styrene acrylic resin with (B) the microbial culture of the present invention or the decomposition composition of the present invention. The present invention relates to a method for decomposing styrene acrylic resin.

  In addition, in this specification, the said product says the product containing resin, especially a styrene acrylic resin, and specifically, a mold form etc. are mentioned.

The degradation method of the present invention has one major feature in that the microorganism culture of the present invention or the degradation composition of the present invention is used. Therefore, according to the decomposition method of the present invention, a resin, particularly a styrene acrylic resin, can be decomposed more stably with higher efficiency without substantially generating CO ₂ . Moreover, according to the decomposition method of the present invention, the resin, particularly styrene acrylic resin, in the waste is preferentially biodegraded and remains by applying it to the treatment of waste including resin, particularly styrene acrylic resin. Unused useful metals, useful compounds, etc. can be separated and / or exposed in a state where they can be easily recovered. Therefore, it becomes possible to more easily recycle unused useful metals and useful compounds in the waste.

  In the decomposition method of the present invention, specifically, the microorganism culture of the present invention or the decomposition composition of the present invention is allowed to express the decomposition activity of the styrene acrylic resin in the presence of the product or waste. Maintaining the suitable conditions, for example, the conditions suitable for the maintenance of each microorganism contained in the microbial culture of the present invention; the microbial culture of the present invention or the decomposing composition of the present invention as the product or waste It may be dispersed or mixed with the product, and maintained under conditions suitable for expressing the degradation activity of the styrene acrylic resin, for example, conditions suitable for maintaining each microorganism contained in the microorganism culture of the present invention. .

  If necessary, the waste may be pulverized by suitable means to form a powder, or may be dissolved in an appropriate solvent that does not hinder the growth of the microorganism culture of the present invention.

  Specific examples of the application of the decomposition method of the present invention include treatment of waste such as toner, can laminate, electronic equipment insulators, home appliance / automobile waste, industrial waste, etc .; resin, particularly styrene acrylic resin Treatment of waste liquid containing; Recovery of useful metals and useful compounds from the waste; When a resin such as styrene acrylic resin is used for molding, for example, a mold made from a resin such as styrene acrylic resin For example, it is easy to recover the target molded product when the material is put in and molded.

  In another aspect of the present invention, (a) a waste material containing a styrene acrylic resin and a metal or a pigment is contacted with (b) the microorganism culture or decomposition composition of the present invention, and styrene in the waste material is brought into contact. The present invention relates to a method for recovering a metal or a dye in waste, which comprises decomposing an acrylic resin and thereby separating the metal or the dye in the waste.

According to the recovery method of the present invention, since the microbial culture or decomposition composition of the present invention is used, the resin in the waste containing the styrene acrylic resin and the metal or the pigment, particularly the styrene acrylic resin is preferentially used. It is possible to separate and / or expose the remaining unused useful metals, useful compounds and the like in a state where they can be easily recovered. Therefore, unused useful metals, useful compounds, etc. in the waste can be easily and inexpensively recovered with low impact on the surrounding environment, and recycling of the useful metals, useful compounds, etc. can be made easier. It becomes possible to do.
Contact between the waste of (a) and the microbial culture or decomposition composition of (b)
1) if necessary, pulverizing the waste by appropriate means to form a powder, or dissolving the waste in an appropriate solvent that does not interfere with the growth of the microbial culture of the present invention; and 2) the microorganism of the present invention. Conditions suitable for expressing the degradation activity of the styrene acrylic resin in the presence of the waste, such as conditions suitable for maintaining each microorganism contained in the microorganism culture of the present invention The step to keep down, or
2 ′) The microorganism culture or decomposition composition of the present invention is dispersed or mixed with the waste, and is included in conditions suitable for expressing the decomposition activity of the styrene acrylic resin, for example, included in the microorganism culture of the present invention. Maintaining under conditions suitable for the maintenance of each microorganism,
It can be done by.

  In the product after decomposition of the styrene acrylic resin contained in the waste, the components composed of the styrene acrylic resin are substantially removed. Therefore, the metal or pigment to be recovered can be easily recovered from the product by a conventional fractionation method, acid-alkali treatment, precipitation, organic solvent extraction, centrifugation, various chromatographies, and the like.

  EXAMPLES Hereinafter, although this invention is demonstrated in detail based on an Example, this invention is not limited to these Examples.

Screening for styrene acrylic resin-degrading microorganisms Styrene acrylic resin (Mikasa Sangyo Co., Ltd., styrene acrylic resin for toner ink) was dissolved in dichloromethane to a final concentration of 5 g / L, and 1 mL of the resulting solution was dispensed into a test tube. . Thereafter, the test tube was air-dried in a fume hood.

In the test tube, FY medium [Composition: 1 wt% NH ₄ Cl, 0.1 wt% KH ₂ PO ₄ , 0.05 wt% K ₂ HPO ₄ , 0.05 wt% MgSO ₄ .7H ₂ O, 0 2 wt% NaCl, 0.001 wt% ZnCl ₂ , 0.001 wt% FeSO ₄ .7H ₂ O, 0.001 wt% CaCl ₂ , 0.1 wt% yeast extract] Autoclaved for minutes.

  Kinki University, Faculty of Agriculture, Soil in the mountains of Nara Prefecture, Soil in Ishikawa Prefecture, Seawater collected from Wakayama Prefecture, Seawater collected from the coast of Osaka Prefecture, Heavy Oil Decomposing Microorganisms Group and Compost preserved by Applied Microbiology Laboratory Was used as a sample. The soil was suspended in physiological saline and then uniformly dispersed by ultrasonic treatment [28 kHz] to prepare a sample. Seawater was used as a sample as it was. Further, the compost was suspended in physiological saline and then uniformly dispersed by ultrasonic treatment [28 kHz] to prepare a sample.

  Each sample was inoculated into the test tube after high-pressure sterilization, and cultured with shaking at 37 ° C. and 125 strokes / min. Samples containing microorganisms that degrade styrene-acrylic resin are those in which the styrene-acrylic resin that has adhered to the test tube wall in the form of a film before culturing is uniformly dispersed in the culture solution, so that the bacteria grow well and the culture solution Was selected as an index. A part of the enrichment culture solution of the sample containing microorganisms that decompose styrene acrylic resin was transferred to a new FY medium, and subculture was repeated.

  As a result, among the screened samples, with respect to the samples derived from compost, as shown in FIG. 1, the styrene acrylic resin adhering to the test tube wall was uniformly dispersed in the culture solution, and the culture solution became cloudy. did. Such a culture containing microorganisms that stably decompose styrene acrylic resin is referred to as AMOC2. The AMOC2 is named and displayed as AMOC2, and is registered at the Patent Microorganism Depositary Center of the National Institute of Technology and Evaluation [Zip No. 292-0818, 2-5-8, Kazusa Kamashichi, Kisarazu City, Chiba Prefecture]. Deposited as P-80 on February 28, 2005 (date of trust).

The obtained AMOC2 was diluted 10-fold with sterilized physiological saline, and R2A plate medium [composition: 0.05% by weight yeast extract, 0.05% by weight peptone, 0.05% by weight casamino acid, 0.05% by weight] % Glucose, 0.05 wt% Soluble starch, 0.03 wt% K ₂ HPO ₄ , 0.005 wt% MgSO ₄ .7H ₂ O, 0.03 wt% Sodium pyruvate, 1.5 wt% Agar; Diff The product was applied to a laboratory (Difco Laboratories) and cultured at 37 ° C. for 7 to 14 days. Thereafter, colonies with different morphologies were selected from the resulting colonies and further selected until they became uniform.

  As a result, 6 types of colony microorganisms shown in FIG. 2 were obtained.

Examination of AMOC2 Properties Each microorganism obtained in Example 1 was treated with an FY medium containing a styrene acrylic resin having a final concentration of 5 g / L [composition: 1 wt% NH ₄ Cl, 0.1 wt% KH ₂ PO ₄ , 0.05 wt% K ₂ HPO ₄ , 0.05 wt% MgSO ₄ .7H ₂ O, 0.2 wt% NaCl, 0.001 wt% ZnCl ₂ , 0.001 wt% FeSO ₄ .7H ₂ O, 0 .001 wt% CaCl ₂ , 0.1 wt% yeast extract] and cultured at 37 ° C. for 7 days.

  Each microorganism after the culture was collected and washed with sterilized water. Thereafter, each microbial cell was crushed by freezing and thawing, and the crushed microbial cell was suspended in 500 μL of TE buffer (composition: 100 mM Tris-HCl, 40 mM EDTA (pH 8.0)). To the resulting suspension, 50 μL of sodium dodecyl sulfate (200 g / L, Wako Pure Chemical Industries, Ltd.) solution and 300 μL of benzyl chloride were added and stirred.

  Thereafter, the obtained product was incubated for 60 minutes in a 50 ° C. water bath with stirring every 5 minutes. To the product obtained after the incubation, 100 mg of zirconia / silica mixed beads (outer diameter 0.1 mm, Biospec Products Inc.) was added, and the mixture was vortexed for 10 seconds. To the obtained product, 300 μL of 3.0 M NaOAc (pH 5.0) was added, and the mixture was allowed to stand in ice water for 30 minutes, and then centrifuged at 17400 × g and 20 ° C. for 10 minutes to recover the supernatant.

  To the obtained supernatant, 600 μL of TE saturated phenol solution was added, and centrifuged at 17400 × g and 20 ° C. for 10 minutes to remove the phenol layer. To the resulting aqueous layer, 600 μL of phenol-chloroform-isoamyl alcohol (50: 49: 1) (hereinafter, PCI) was added, and centrifuged at 17400 × g, 20 ° C. for 10 minutes to recover the upper layer (aqueous layer). . To the recovered aqueous layer, 600 μL of chloroform: isoamyl alcohol / 24: 1 (v / v) (CIA) was added and centrifuged at 17400 × g and 20 ° C. for 10 minutes to recover the supernatant.

  To the obtained supernatant, 600 μL of 2-propanol was added and allowed to stand at 4 ° C. for 10 minutes. Thereafter, the obtained product was centrifuged at 17400 × g and 20 ° C. for 20 minutes, and the supernatant was removed. The obtained precipitate was washed with 800 μL of 70% (v / v) ethanol, and then the ethanol was removed and the precipitate was air-dried. The obtained product was dissolved in 20 mL of sterilized water to obtain a DNA solution. The obtained DNA solution was diluted 50 times with sterilized water, and the absorbance at 260 nm of the obtained solution was measured with a spectrophotometer [trade name: DU. Series 650, manufactured by Beckman Co., Ltd.] to determine the DNA concentration. The obtained DNA was used as template DNA.

Using the template DNA, a 200 bp base sequence corresponding to the V3 region of 16S rDNA (corresponding to positions 341 to 536 of E. coli 16S rDNA) was amplified by PCR. Specifically, using a product name: MyCycler Thermal Cycler (manufactured by BioRad (BIO RAD)), 10 × Gold Taq buffer (trade name, manufactured by Applied Biosystems) 5 μL and 2.0 mM dNTP mixture [Applied Biosystems (Applied Biosystems)] 5 μL, 25 mM MgCl ₂ [Applied Biosystems (Applied Biosystems) 6 μL, forward primer GC-341F: 5′-CGCCCGCCGCGCCCCGGGCCCGCCCCGCCGCCCCCG Genosys, SEQ ID NO: 1) 1 μL and reverse primer 534R [5′-ATTACCGCGGCTGCTGG-3 ′ (10 pmol / μL, Sigma Genosys) SEQ ID NO: 2) Reaction solution containing 1 μL, 50 ng equivalent amount of template DNA, AmpliTaq Gold [5 U / μL, Applied Biosystems, Inc.] 0.2 μL, and the remainder of sterile water PCR was performed in 50 μL.

  The PCR thermal profile consists of 25 cycles of 10 cycles of incubation at 95 ° C. followed by one cycle of heat denaturation at 93 ° C. for 60 seconds, annealing at 48 ° C. for 70 seconds, and extension at 72 ° C. for 70 seconds. Incubation at 5 ° C. for 5 minutes.

  The obtained product was subjected to agarose gel electrophoresis to confirm amplification of the target sequence. The obtained product was subjected to a product name: QIAEXII Gel Extraction Kit (manufactured by QIAGEN) and concentrated to obtain a denaturant gradient gel electrophoresis (DGGE) analysis target sample.

  Subsequently, denaturant concentration gradient gel electrophoresis was performed on the obtained DGGE analysis target sample using a trade name: Dcode system 6 (manufactured by Bio-Rad Lab.).

  Denaturant gradient includes 7.0M urea (Bio-Rad Lab.) And 40% (v / v) formamide (Bio-Rad Lab.). Was adjusted to 20% to 50% in the electrophoresis direction, with a concentration of 100%. In addition, a polyacrylamide gel in which the concentration of acrylamide (manufactured by Bio-Rad Lab.) Was 6 to 9% (v / v) in the electrophoresis direction was used. The electrophoresis conditions were a voltage of 200 V, a migration time of 6 hours, a migration temperature of 61 ° C., and 0.5 × TAE buffer was used as a migration buffer.

  After completion of the electrophoresis, the gel was stained with a trade name: Syber Gold (manufactured by Takara Bio Inc.) for 30 minutes. Thereafter, the gel was irradiated with ultraviolet rays (310 nm), and excitation light based on the stained DNA was subjected to trade name: Kodak Gel Logic 200 Imaging System (manufactured by Kodak) and trade name: KODAK GL Filter (SYBR Green). For SYBR Gold, manufactured by Kodak Co., Ltd.]. The results are shown in FIG.

  As shown in FIG. 3, a plurality of DNA bands were confirmed from AMOC2, and the DNA bands Q-1 to Q-6 could be attributed to the DNA bands derived from AMOC2, respectively.

  Further, the base sequence of 16S rDNA was analyzed by PCR using the template DNA derived from Q-1 to Q-6 as follows.

Using a product name: MyCycler Thermal Cycler (manufactured by BioRad (BIO RAD)), 5 μL of 10 × Gold Taq buffer (trade name, Applied Biosystems) and 2.0 μm each of dNTP mixture 5 μL, 6 μL of 25 mM MgCl ₂ and forward primer 27f: 5′-AGAGTTTGATCCTGGCTCAG-3 ′ (10 pmol / μL, manufactured by Toagosei Co., Ltd., SEQ ID NO: 3) 1 μL and reverse primer 1525r: 5′-AAAGGAGGTGATCCAGCC-3 ′ (10 pmol / μL, manufactured by Toa Gosei Co., Ltd., SEQ ID NO: 4) 1 μL, 50 ng equivalent amount of the template DNA, and trade name: AmpliTaq Gold [5 U / μL, manufactured by Applied Biosystems] 0.5 μL And a PCR reaction solution in the reaction solution 50μL containing the sterile water balance.

  The PCR thermal profile consists of 25 cycles of 7 minutes incubation at 94 ° C. followed by 45 cycles of heat denaturation at 94 ° C. for 45 seconds, annealing at 55 ° C. for 45 seconds, and extension at 72 ° C. for 45 seconds, 72 Incubation was at 10 ° C. for 10 minutes. The obtained product was subjected to agarose gel electrophoresis to confirm amplification of the target sequence. The obtained product was purified using 1.5 wt% low melting point agarose gel (manufactured by BioProducts) and dissolved in 20 μL of sterilized water to obtain a sample for sequencing.

Direct sequencing was performed using the sequencing sample. For the extension reaction, a trade name: DYEamic ET Terminator Cycle Sequencing Kit (manufactured by Amersham Biosciences) was used, and the following 16S rDNA sequence primer:
f1L forward primer 9-27 5′-GAGTTTGATCCTGGCTCAG-3 ′ (SEQ ID NO: 5),
f2L forward primer 518-536 5′-CCAGCAGCCGCGGTAATAC-3 ′ (SEQ ID NO: 6),
f2.5L forward primer 776-795 5'-AGCAAACAGGATTAGATACC-3 '(SEQ ID NO: 7),
f3L forward primer 1093-1111 5′-GTCCCGCAACGAGCGCAAC-3 ′ (SEQ ID NO: 8),
r1L reverse primer 535-517 5′-GTATTACCGCGGCTGCTGG-3 ′ (SEQ ID NO: 9),
r2L reverse primer 804-785 5′-GACTACCAGGGTATCTAATC-3 ′ (SEQ ID NO: 10),
r3L reverse primer 1110-1092 5′-TTGCGCTCGTTGCGGGACT-3 ′ (SEQ ID NO: 11),
r4L reverse primer 1405-1388 5′-ACGGGCGGTGTGTACAAG-3 ′ (SEQ ID NO: 12),
rE1L reverse primer 344-326 5′-GTAGGAGTCTGGACCGTGT-3 ′ (SEQ ID NO: 13),
Was used. The sequence reaction conditions were 30 cycles with 95 ° C. for 20 seconds, 50 ° C. for 15 seconds and 60 ° C. for 1 minute as one cycle. Subsequently, each sample was analyzed using a DNA sequencer (trade name: ABI PRISM 3100 DNA Sequencer, Applied Biosystems).

  About the obtained 16S rDNA base sequence to be analyzed, BLAST (Basic Local Alignment Search Tool) is used for the 16S rDNA library registered in the database of DNA Data Bank of Japan (DDBJ), and the search target division bacteria, A strain with high homology was searched under the conditions of GAP calculation, expected value 10 and other parameters default values.

  As a result, the Q-1 was 98.6% homologous with the Luteimonas sp. TUT1238, the Q-2 was 98.6% homologous with the Mesohizobium loti, the Q- 3 is 96.5% of homology with Rocharimaea elizabethae, and Q-4 is 96.6% of homology with Rochalimaea elisabethee, and Q-5 is p ) And 99.5% homology, and Q-6 showed 99.6% homology with Alcaligenes sp. Mp-2.

  Therefore, Q-1 is referred to as Luteimonas sp. Q-1, Q-2 as Mesohizobium loti Q-2, Q-5 as Pseudomonas boreopolis Q-, Pseudomonas boreeopolis Q-. The above Q-6 was designated as Alcaligenes sp. Q-6.

  Subsequently, the morphological and physiological biochemical characteristics of Q-1 to Q-6 were examined by a conventional method. About said Q-1-Q-6, it observed with the phase-contrast microscope [made by Nikon Corporation], and examined the mobility. In addition, Gram staining and oxidase test were performed according to B. Holmes et al. [International Journal of Systemic Bacteriology, 38, 406-416, (1988)].

  As a result, Q-1, Q-2, Q-3, Q-4, Q-5 and Q-6 were all Gram-negative short bacillus having motility.

  Further, other physiological and biochemical properties were examined using a trade name: Api 20E identification kit [manufactured by BioMerieux]. Table 1 shows the physiological and biochemical properties.

Decomposition activity of styrene acrylic resin Dissolve the styrene acrylic resin (Mikasa Sangyo Co., Ltd., styrene acrylic resin for toner ink) in dichloromethane to a final concentration of 5 g / L, and dispense 1 mL of the resulting solution into a test tube. did. Thereafter, the test tube was air-dried in a fume hood.

In the test tube, FY medium [Composition: 1 wt% NH ₄ Cl, 0.1 wt% KH ₂ PO ₄ , 0.05 wt% K ₂ HPO ₄ , 0.05 wt% MgSO ₄ .7H ₂ O, 0 2 wt% NaCl, 0.001 wt% ZnCl ₂ , 0.001 wt% FeSO ₄ .7H ₂ O, 0.001 wt% CaCl ₂ , 0.1 wt% yeast extract] Autoclaved for minutes.

  Subsequently, each of AMOC2, Q-1 to Q-6 was inoculated into the test tube after autoclaving, and cultured with shaking at 37 ° C. and 125 strokes / min. As a control, a polyester compound-degrading microorganism group AMOC001 stored in the Applied Microbiology Laboratory of the Faculty of Agriculture, Kinki University was used.

  The styrene acrylic resin remaining in the culture broth obtained after 7 days of culture and the degradation products derived from it are extracted with dichloromethane, and hexatriacontane (internal standard, Sigma-Aldrich) is added. And dried under reduced pressure using an evaporator. The obtained dried product was dissolved in 5 mL of hexane: toluene / 2: 8 (volume ratio).

  1 μL of the obtained sample was spotted on a rod (trade name: Chromarod SIII, manufactured by Mitsubishi Chemical Yatron Co., Ltd.) thinly coated with silica gel around quartz glass, and the rod was placed in a humidity chamber (condition: 65% RH). For 10 minutes. Then, after hanging for 10 minutes in the developing tank containing n-hexane, it was developed 10 cm. In addition, hanging and expansion | deployment were performed in a 37 degreeC thermostat.

  The developed rod was dried at room temperature to remove the solvent, and the rod developed 10 cm was first subjected to FID analysis to detect an internal standard peak.

  Thereafter, the rod for which the partial measurement was completed was kept again in the constant humidity chamber for 10 minutes. 10 cm was developed with toluene. In order to remove, the solvent was removed by drying at room temperature. Using the developed rod, trade name: Iatroscan Mk-6s (manufactured by Mitsubishi Chemical Yatron Co., Ltd.), detector: FID, hydrogen gas: 160 mL / min, air: 2.0 L / min, scan speed: Analysis was performed under the condition of 30 seconds / scan, and a peak corresponding to the styrene acrylic resin and a peak corresponding to the decomposition product derived therefrom were detected. Further, subculture was performed every 7 days, and the change with time of the degradation rate was also determined.

  As a negative control, the same procedure was performed in the absence of a microorganism culture.

  The degradation activity is determined by comparing the area of each peak in the presence of the microorganism culture with the area of each peak in the control, and when the area of the peak corresponding to the styrene acrylic resin is zero, the styrene acrylic resin Was calculated as an indicator that 100% was decomposed. The results are shown in FIGS.

  As a result, as shown in FIG. 4, it can be seen that AMOC2 is stable and has a decomposition rate of about 45% even when subcultured for 3 or more generations, and can decompose styrene acrylic resin. The growth was also good. However, as shown in FIG. 4, in the polyester-degrading microorganism group AMOC001, the degradation rate of the styrene acrylic resin decreases as the number of passages increases, and the degradation of the resin also occurs at the third passage number (7 days / times). The growth of fungus was not recognized.

  In addition, as shown in FIG. 5, the Q-1 to Q-4 and Q-6 strains alone had a degradation rate of 25%, but the Q-5 strain was stable and degraded. The rate was about 40%. Therefore, in AMOC2, it is suggested that Q-5 cooperates with Q-1 to Q-4 and Q-6 to complement each other's functions and maintain a high decomposition rate.

  According to the present invention, waste containing styrene acrylic resin can be treated at low cost and with a low impact on the environment, and the metal or pigment in the waste can be treated at a low cost and with a low impact on the environment. It can be recovered.

FIG. 1 is a diagram illustrating the degradation of styrene acrylic by a microbial culture. Lane 1 is a control and lane 2 shows the degradation of styrene acrylic in the presence of a microbial culture.

FIG. 2 is a diagram showing the shape of the colonies Q-1 to Q-6.

FIG. 3 is a diagram showing the results of denaturant concentration gel electrophoresis analysis of 16S rDNA V3 regions of AMOC2 and Q-1 to Q-6, respectively. Lane 1 is AMOC2, and Lanes 2 to 7 are Q-1 to Q-6, respectively.

FIG. 4 is a diagram showing the results of examining the decomposition activity of styrene acrylic resin by AMOC2 and AMOC001. In the figure, the square is AMOC2, and the triangle is AMOC001.

FIG. 5 is a diagram showing the results of examining the decomposition activity of styrene acrylic resin by AMOC2 and Q-1 to Q-6, respectively. In the figure, the square is AMOC2, the star is Q-1, the cross is Q-2, the pentagon is Q-3, the hexagon is Q-4, the rhombus is Q-5, the triangle is Q -6.

  SEQ ID NO: 1 is the sequence of the primer.

  SEQ ID NO: 2 is the primer sequence.

  SEQ ID NO: 3 is the primer sequence.

  SEQ ID NO: 4 is the sequence of a primer.

  SEQ ID NO: 5 is the sequence of the primer.

  SEQ ID NO: 6 is the sequence of the primer.

  SEQ ID NO: 7 is the primer sequence.

  SEQ ID NO: 8 is the sequence of a primer.

  SEQ ID NO: 9 is the sequence of a primer.

  SEQ ID NO: 10 is a primer sequence.

  SEQ ID NO: 11 is the sequence of a primer.

  SEQ ID NO: 12 is the sequence of a primer.

  SEQ ID NO: 13 is the sequence of a primer.

Claims (8)

  A microorganism culture exhibiting a biodegradability of styrene acrylic resin, comprising microorganisms belonging to Pseudomonas boreeopolis.   The microorganism culture according to claim 1, wherein the microorganism belonging to Pseudomonas boreopolis is Pseudomonas boreopolis Q-5 (accession number: NITE P-80).   A microorganism belonging to Luteimonas sp., A microorganism belonging to Mesorhizobium loti, a microorganism belonging to Rocharmaea elizabethee, and a microorganism belonging to Algae sp. The microorganism culture according to claim 1 or 2, wherein   The composition for decomposition | disassembly of a styrene acrylic resin formed by containing the microorganism culture of any one of Claims 1-3.   The composition for decomposing a styrene acrylic resin according to claim 4, further comprising a fixing carrier suitable for holding a microorganism culture.   The composition for decomposing a styrene acrylic resin according to claim 4 or 5, further comprising a medium suitable for culturing microorganisms.   (A) A product or waste containing a styrene acrylic resin, and (B) the microorganism culture according to any one of claims 1 to 3 or the decomposition composition according to any one of claims 4 to 6. A method for decomposing a styrene acrylic resin, wherein   (A) waste containing a styrene acrylic resin and a metal or pigment; and (b) the microorganism culture according to any one of claims 1 to 3 or the degradation according to any one of claims 4 to 6. A method for recovering a metal or pigment in waste, comprising contacting the composition to decompose styrene acrylic resin in the waste, thereby separating the metal or pigment in the waste.