JP2006296429A5 - - Google Patents

Claims (29)

The human gene which codes polypeptide of the following (a) or (b).
(A) A polypeptide comprising the amino acid sequence represented by SEQ ID NO: 2.
(B) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (a) and having an angiogenesis inhibitory action. The human gene which codes polypeptide of the following (c) or (d).
(C) A polypeptide comprising the amino acid sequence of amino acid numbers 202 to 317 represented by SEQ ID NO: 2.
(D) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (c) and having an angiogenesis inhibitory action. A mouse gene encoding the following polypeptide (e) or (f).
(E) A polypeptide comprising the amino acid sequence represented by SEQ ID NO: 4.
(F) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (e) and having an angiogenesis inhibitory action. A rat gene encoding the following polypeptide (g) or (h).
(G) A polypeptide comprising the amino acid sequence represented by SEQ ID NO: 6.
(H) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (g) and having an angiogenesis inhibitory action.   The human gene according to claim 1, comprising the base sequence represented by SEQ ID NO: 1.   The isolated human gene according to claim 2, comprising the nucleotide sequence of nucleotide sequence numbers 670 to 1017 represented by SEQ ID NO: 1.   The mouse gene according to claim 3, comprising the base sequence represented by SEQ ID NO: 3.   The rat gene according to claim 4, comprising the base sequence represented by SEQ ID NO: 5. A polypeptide encoded by a human gene of the following (i) or (j):
(I) A polypeptide comprising the amino acid sequence represented by SEQ ID NO: 2.
(J) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (i) and having an angiogenesis inhibitory action. The polypeptide of the following (k) or (l) encoded by a human gene.
(K) A polypeptide comprising the amino acid sequence of amino acid numbers 202 to 317 represented by SEQ ID NO: 2.
(L) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (k) and having an angiogenesis inhibitory action. The polypeptide of the following (m) or (n) encoded by a mouse gene.
(M) A polypeptide comprising the amino acid sequence represented by SEQ ID NO: 4.
(N) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (m) and having an angiogenesis inhibitory action. The following polypeptide encoded by the rat gene (o) or (p):
(O) A polypeptide comprising the amino acid sequence represented by SEQ ID NO: 6.
(P) A polypeptide comprising an amino acid sequence in which one or several amino acids are deleted, substituted or added in the polypeptide (o) and having an angiogenesis inhibitory action.   An oligonucleotide probe that hybridizes with at least a portion of the gene according to any one of claims 1-8.   A gene detection reagent comprising the oligonucleotide probe according to claim 13.   Recombinant DNA containing the gene according to any one of claims 1 to 8.   A transformant transformed with the recombinant DNA according to claim 15.   A method for producing the polypeptide, comprising culturing the transformant according to claim 16 and collecting a polypeptide encoded by a human, mouse, or rat gene from the obtained culture.   A monoclonal antibody or an antibody fragment thereof that specifically reacts with the polypeptide according to any one of claims 9 to 12.   A monoclonal antibody or an antibody fragment thereof which specifically reacts with a polypeptide comprising the amino acid sequence of amino acid numbers 245 to 252 of SEQ ID NO: 2.   A polyclonal antibody or an antibody fragment thereof that specifically reacts with the polypeptide according to any one of claims 9 to 12.   A polyclonal antibody or an antibody fragment thereof that specifically reacts with a polypeptide comprising the amino acid sequence of amino acid numbers 245 to 252 of SEQ ID NO: 2.   A hybridoma producing a monoclonal antibody, which is obtained by fusing the antibody-producing cell producing the antibody according to claim 18 or 19 and a myeloma cell.   A diagnostic kit comprising the polypeptide according to any one of claims 9 to 12, and the monoclonal antibody according to claim 18 or 19 and / or the polyclonal antibody according to claim 20 or 21.   A pharmaceutical composition comprising the polypeptide according to any one of claims 9 to 12.   A pharmaceutical composition comprising the monoclonal antibody according to claim 18 or 19, or the polyclonal antibody according to claim 20 or 21.   A pharmaceutical composition comprising an antisense oligonucleotide that specifically hybridizes with a part of the gene according to claim 1.   A pharmaceutical composition comprising a nucleic acid that can be used for gene therapy, comprising at least a part of the gene according to claim 1.   An angiogenesis inhibitor comprising the polypeptide according to any one of claims 9 to 12.   An angiogenesis inhibitor comprising the gene according to any one of claims 1 to 8.