JP2006292581A - Solublity displaying method - Google Patents
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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Abstract
Description
本発明は、溶解性表示方法に関する。更に詳述すれば、本発明は、水又は水溶液への溶質の溶解性を評価するのに有用な溶解性表示方法に関する。特に、本発明の溶解性表示方法は、水又は水溶液中の水の解離度を表わす指標として有用である。 The present invention relates to a solubility display method. More specifically, the present invention relates to a solubility display method useful for evaluating the solubility of a solute in water or an aqueous solution. In particular, the solubility display method of the present invention is useful as an index representing the degree of dissociation of water in water or an aqueous solution.
本発明の溶解性表示方法は、水又は水溶液が電解生成水の場合特に好ましく適用できる。この電解生成水は、例えば、飲用、食品加工用、薬用、化粧品用、洗剤用等に使用される。また、本発明における上記の指標で、電解装置が製造する電解水がどの程度の解離度の電解水を製造できる能力を持っているかを定量的に表示できる。 The solubility display method of the present invention can be applied particularly preferably when the water or aqueous solution is electrolyzed water. This electrolyzed water is used, for example, for drinking, food processing, medicinal use, cosmetic use, detergent use, and the like. In addition, the above index of the present invention can quantitatively display the degree of dissociation of the electrolyzed water produced by the electrolyzer.
希薄な電解質水溶液を電解すると両極に電解生成水が生成する。陽極側の電解生成水は殺菌作用やアストリンゼント効果があるので、薬品や化粧品用に使用される。一方、陰極側の電解生成水は飲用や食品加工等に使用される。また、陰極側の電解生成水は油脂や蛋白質をよく溶かす特性があるので、食器洗浄用などにも使用される。これらの用途では、電解生成水の溶質溶解性を計測し、これを定量的に表示することが、製品製造用の電解生成水を選択する上で、或は製造工程を管理する上で重要である。 Electrolysis of a dilute electrolyte aqueous solution produces electrolyzed water at both electrodes. The electrolyzed water on the anode side is used for chemicals and cosmetics because it has a bactericidal action and an astringent effect. On the other hand, the electrolyzed water on the cathode side is used for drinking and food processing. Further, the electrolyzed water on the cathode side has a property of dissolving oils and fats and proteins well, so it is also used for washing dishes. In these applications, measuring the solute solubility of electrolyzed water and displaying it quantitatively is important in selecting electrolyzed water for product production or managing the manufacturing process. is there.
電解生成水の特性を表すパラメータとして、水素イオン濃度（ｐＨ）、酸化還元電位（ＯＲＰ）、溶存酸素濃度（ＤＯ）或は電気伝導度（ＥＣ）が従来から知られている。しかしながら、これらのパラメータは全て溶質の濃度に依存するものであり、水自体の特性を表わすパラメータではない。このため、これらのパラメータでは電解生成水の溶質溶解性を正確には表示できない。例えば、従来水又は水溶液はｐＨが高い程溶質の溶解性が高いとされている。しかしながら、アルカリ性の陰極側電解生成水を酸により中和したものは、ｐＨが比較的低いにもかかわらず水の解離が高く溶質の溶解性が高い。従って、ｐＨは水又は水溶液の溶質溶解性を表すパラメータとしては適切でない。この場合と同様にｐＨ以外の上記パラメータについても単独では水又は水溶液の溶質溶解性を表示することが難かしい。結局、水又は水溶液中の水自体の解離特性を加味しないと、水又は水溶液の溶質溶解性を正確に表示することが困難である。 As parameters representing the characteristics of electrolyzed water, hydrogen ion concentration (pH), oxidation-reduction potential (ORP), dissolved oxygen concentration (DO) or electrical conductivity (EC) are conventionally known. However, these parameters all depend on the concentration of the solute, and do not represent the characteristics of water itself. For this reason, the solute solubility of electrolyzed water cannot be accurately displayed with these parameters. For example, conventionally, water or an aqueous solution is considered to have higher solubility of solute as pH is higher. However, water obtained by neutralizing alkaline cathode-side electrolyzed water with an acid has high dissociation of water and high solubility of solutes even though the pH is relatively low. Accordingly, pH is not appropriate as a parameter representing the solute solubility of water or an aqueous solution. As in this case, it is difficult to display the solute solubility of water or an aqueous solution alone for the above parameters other than pH. After all, it is difficult to accurately display the solute solubility of water or an aqueous solution unless the dissociation characteristics of the water or the aqueous solution itself is taken into account.
水に対する溶質の溶解度は、一般に溶解温度が高いほど高くなる。この現象は、水分子の解離が高温度程顕著になることによる。電場、磁場等のエネルギーを水に加えると、水分子の解離が進み溶質を溶かしやすい性質に改質される。特に、電解生成水が溶質を溶かしやすい。 The solubility of solutes in water generally increases as the dissolution temperature increases. This phenomenon is due to the fact that the dissociation of water molecules becomes more prominent at higher temperatures. When energy such as an electric field or a magnetic field is added to water, the dissociation of water molecules proceeds and the solute is easily dissolved. In particular, electrolyzed water tends to dissolve solutes.
水分子の解離と溶質溶解速度との関係は、ある一定範囲においては比例的に成立する。また、超臨界水では水分子の解離が最大の状態に到達し、溶質の溶解が極限を示すと推察される。このように、水の解離度を計測することが水又は水溶液の溶質溶解性を表示するパラメータとして重要である。 The relationship between the dissociation of water molecules and the solute dissolution rate is proportionally established within a certain range. In supercritical water, the dissociation of water molecules reaches the maximum state, and it is assumed that the dissolution of solutes shows the limit. Thus, measuring the degree of dissociation of water is important as a parameter indicating the solute solubility of water or an aqueous solution.
塩化ナトリウムのような電解質の水溶液を電解すると、電解エネルギーに比例して水の解離が進むことが知られている（非特許文献１）。しかし、このような水溶液中の水の解離度を迅速に計測する方法は、未だ見出されていない。このため、電解質水溶液中の水の解離度の簡便計測方法、或は解離度に対応する溶解特性の簡便計測方法の開発が切望されている。
本発明は上記事情に鑑みなされたもので、その目的とするところは、水又は水溶液の溶質溶解性評価に有用な溶解性表示方法を提供することにある。特に、飲用、食品加工用、薬用、化粧品用、洗剤用等に用いられる水又は水溶液中の水の解離度に対応する溶解特性の簡便計測方法を提供することにある。 This invention is made | formed in view of the said situation, The place made into the objective is to provide the solubility display method useful for the solute solubility evaluation of water or aqueous solution. In particular, it is to provide a simple measurement method of solubility characteristics corresponding to the degree of dissociation of water in water or an aqueous solution used for drinking, food processing, medicinal use, cosmetic use, detergent use and the like.
本発明は以下に記載するものである。 The present invention is described below.
〔１〕 ｐＨが５．５から９．５であり、所定温度に保たれた測定対象の水又は水溶液にＤ−グルコースを添加し、添加してから所定時間経過後に該水又は水溶液中に溶解する単分子状Ｄ−グルコース濃度を測定し、該濃度の測定結果から算出した単分子状Ｄ−グルコースの溶解速度を該水又は水溶液の溶質溶解性を示すパラメータとすることを特徴とする溶解性表示方法。
〔２〕水又は水溶液中に溶解する単分子状Ｄ−グルコース濃度を酵素電極法により測定する〔１〕に記載の溶解性表示方法。
〔３〕 酵素電極法が、フェリシアンイオンを添加してなる反応系を用いたグルコースオキシダーゼアンペロメトリー法である〔２〕に記載の溶解性表示方法。
〔４〕 水又は水溶液が電解生成水である〔１〕に記載の溶解性表示方法。
〔５〕 Ｄ−グルコースの添加量が測定対象の水又は水溶液１００ｍｌ当たり１００〜６００ｍｇである〔１〕に記載の溶解性表示方法。
〔６〕 所定温度が２０〜３０℃である〔１〕に記載の溶解性表示方法。
〔７〕 所定時間が１〜６０分である〔１〕に記載の溶解性表示方法。
[1] pH is 5.5 to 9.5, and D-glucose is added to water or an aqueous solution to be measured which is kept at a predetermined temperature, and dissolved in the water or aqueous solution after a predetermined time has elapsed since the addition. The solubility is characterized by measuring the concentration of monomolecular D-glucose to be measured, and using the dissolution rate of monomolecular D-glucose calculated from the measurement result of the concentration as a parameter indicating the solute solubility of the water or aqueous solution. Display method.
[2] The solubility display method according to [1], wherein a monomolecular D-glucose concentration dissolved in water or an aqueous solution is measured by an enzyme electrode method.
[3] The solubility display method according to [2], wherein the enzyme electrode method is a glucose oxidase amperometry method using a reaction system in which ferricyan ions are added.
[4] The solubility indication method according to [1], wherein the water or the aqueous solution is electrolytically generated water.
[5] The solubility display method according to [1], wherein the amount of D-glucose added is 100 to 600 mg per 100 ml of water or an aqueous solution to be measured.
[6] The solubility indication method according to [1], wherein the predetermined temperature is 20 to 30 ° C.
[7] The solubility indication method according to [1], wherein the predetermined time is 1 to 60 minutes.
本発明の溶解性表示方法によれば、水又は水溶液の溶質溶解性を正確に評価できる。特に、本発明の溶解性表示方法を用いれば、飲用、食品加工用、薬用、化粧品用、洗剤用等の製造に用いる水又は水溶液を容易に選択できる。或はこれらの製品の製造工程を容易に管理できる。 According to the solubility display method of the present invention, the solute solubility of water or an aqueous solution can be accurately evaluated. In particular, if the solubility display method of the present invention is used, it is possible to easily select water or an aqueous solution used for production of drinks, food processing, medicinal products, cosmetics, detergents and the like. Alternatively, the manufacturing process of these products can be easily managed.
本発明の溶解性表示方法は、測定対象の水又は水溶液中の水の解離度を表わす指標として優れる。即ち、この指標で電解装置が製造する電解生成水（電解水）がどの程度の解離度の電解水を製造できる能力を持っているかを定量的に表示できる。 The solubility display method of the present invention is excellent as an index representing the degree of dissociation of water to be measured or water in an aqueous solution. That is, it is possible to quantitatively display the degree of dissociation of electrolyzed water (electrolyzed water) produced by the electrolyzer using this index.
（原理）
水又は水溶液への溶質の溶解速度は、水又は水溶液中の水の解離が大きいほど大きい。従って、水又は水溶液中の水の解離特性を計測できれば、水又は水溶液への溶質溶解性を評価することができる。
(principle)
The dissolution rate of a solute in water or an aqueous solution increases as the dissociation of water in the water or aqueous solution increases. Therefore, if the dissociation characteristics of water in water or aqueous solution can be measured, solute solubility in water or aqueous solution can be evaluated.
一般にグルコースは水に溶解すると水分子のＨ基とグルコース分子中の５つのＯＨ基とが水素結合し、構造化して熱力学的に安定化する。グルコース分子にはいくつかの異性体が知られている。例えば、ピラノース型、フラノース型、開環型、α体、β体が知られている。水はこのような異性体を含むグルコース分子と構造化し、グルコース自身が保水する。この特性は保水性を示すという理由で化粧品に用いられている。 In general, when glucose is dissolved in water, the H group of the water molecule and the five OH groups in the glucose molecule are hydrogen-bonded and structured and thermodynamically stabilized. Several isomers are known for the glucose molecule. For example, pyranose type, furanose type, ring-opening type, α-form and β-form are known. Water is structured with glucose molecules containing such isomers, and glucose itself retains water. This property is used in cosmetics because it exhibits water retention.
上記グルコースの異性体のうち、右旋性のＤ−グルコースは、これを水に添加した場合、比較的迅速にクラスター（単分子に分離していない状態で水溶液中に溶解しているＤ−グルコース分子集合体）として水に溶解する。このクラスターは、水中で更に単分子状Ｄ−グルコース（単分子の状態で水溶液中に溶解し自由運動しているＤ−グルコース単分子）に変わるが、Ｄ−グルコースの単分子としての水への溶解速度は小さく、単分子として溶解するまでに長時間を要する。このＤ−グルコースが単分子になって水溶解するＤ−グルコースの溶解速度は、水の解離度に大きく依存する。本発明者は、このようなＤ−グルコース単分子の水への溶解速度の小さい特性を利用してこれを計測すれば、比較的簡単に水溶液中の水の解離度を示す指標として溶解特性で表示できることを見出した。 Of the above isomers of glucose, dextrorotatory D-glucose, when added to water, is relatively quickly clustered (D-glucose dissolved in an aqueous solution in a state where it is not separated into single molecules). It dissolves in water as a molecular assembly. This cluster is further converted into monomolecular D-glucose (D-glucose monomolecule which is dissolved in an aqueous solution in a monomolecular state and freely moves in water) in water. The dissolution rate is small, and it takes a long time to dissolve as a single molecule. The dissolution rate of D-glucose in which D-glucose becomes a single molecule and dissolves in water greatly depends on the degree of dissociation of water. If the present inventor measures this by utilizing such a characteristic that the D-glucose monomolecule has a low dissolution rate in water, it can be used as an index indicating the degree of dissociation of water in an aqueous solution relatively easily. I found that it can be displayed.
即ち、上記の性質を利用して、測定対象の水又は水溶液について任意の一定温度下で、単分子状Ｄ−グルコースの溶解速度を測定することにより、解離度を示す指標である溶解特性が求まる。具体的には、所定ｐＨで、所定温度に保たれている測定対象の水又は水溶液に、所定量の固体状Ｄ−グルコースを添加し、正確に所定時間経過後に水又は水溶液中に溶解している単分子状Ｄ−グルコース濃度をクラスター濃度と区別して測定する。 That is, by utilizing the above-mentioned properties, the dissolution characteristics as an index indicating the degree of dissociation can be obtained by measuring the dissolution rate of monomolecular D-glucose at an arbitrary constant temperature for the water or aqueous solution to be measured. . Specifically, a predetermined amount of solid D-glucose is added to water or an aqueous solution to be measured which is maintained at a predetermined temperature at a predetermined pH, and is dissolved in water or an aqueous solution exactly after a predetermined time. The unimolecular D-glucose concentration is measured separately from the cluster concentration.
単分子状Ｄ−グルコース濃度の測定は、公知の酵素電極法で行うことができる。特に、グルコースオキシターゼ（ＧＯＤ）を用いる酵素電極法で行うことが好ましい。この方法では、ＧＯＤとフェリシアン化カリウムを含むバイオセンサー内で単分子状に溶解しているＤ−グルコース分子のみがＧＯＤと特異的に反応し、グルコン酸と電子とを発生する。そして、フェリシアン化カリウムの一部がフェロシアン化カリウムとなる。この系に一定電圧を印加すると、生成したフェロシアン化カリウムが還元され再びフェリシアン化カリウムになる。このときの電流値が溶解している単分子状Ｄ−グルコースの濃度と比例することから、電流量を計測することによって単分子状Ｄ−グルコースの濃度を計測できる。この方法ではクラスター状のＤ−グルコースは計測されない。 The monomolecular D-glucose concentration can be measured by a known enzyme electrode method. In particular, the enzyme electrode method using glucose oxidase (GOD) is preferable. In this method, only D-glucose molecules dissolved in a single molecule in a biosensor containing GOD and potassium ferricyanide react specifically with GOD to generate gluconic acid and electrons. And a part of potassium ferricyanide turns into potassium ferrocyanide. When a constant voltage is applied to this system, the produced potassium ferrocyanide is reduced to become potassium ferricyanide again. Since the current value at this time is proportional to the concentration of dissolved monomolecular D-glucose, the concentration of monomolecular D-glucose can be measured by measuring the amount of current. With this method, clustered D-glucose is not measured.
以下にその反応式を示す。 The reaction formula is shown below.
Ｃ6Ｈ12Ｏ6 ＋ ２[Ｆｅ(III)(ＣＮ)6]3- ＋ Ｈ2Ｏ
→ Ｃ6Ｈ12Ｏ7 ＋ ２[Ｆｅ(II)(ＣＮ)6]4- ＋ ２Ｈ+ （１）
２[Ｆｅ(II)(ＣＮ)6]4- → ２[Ｆｅ(III)(ＣＮ)6]3- ＋ ２ｅ+ （２）
２Ｈ+ ＋ １／２Ｏ2 ＋ ２ｅ+ → Ｈ2Ｏ （３）
上記の酸化還元反応の式（２）、式（３）に示される生成電子（２ｅ+）の量である電流値を計測することにより、水溶液中の単分子状Ｄ−グルコース量を計測することができる。
C 6 H 12 O 6 +2 [Fe (III) (CN) 6 ] 3- + H 2 O
→ C 6 H 12 O 7 +2 [Fe (II) (CN) 6 ] 4- + 2H + (1)
2 [Fe (II) (CN) 6 ] 4- → 2 [Fe (III) (CN) 6 ] 3- + 2e + (2)
2H + + 1 / 2O 2 + 2e + → H 2 O (3)
Measure the amount of monomolecular D-glucose in the aqueous solution by measuring the current value, which is the amount of the generated electrons (2e + ) shown in the above formulas (2) and (3) of the redox reaction. Can do.
ＧＯＤ法により単分子状Ｄ−グルコースの濃度を計測する場合、ｐＨの影響がどの程度であるかを予め把握しておく必要がある。本発明者は純水にＮａＯＨ或は塩酸を加えて種々のｐＨに調整した水溶液を用いて、単分子状Ｄ−グルコースの溶解速度のｐＨの依存性について検討した。その結果、ｐＨが５．５から９．５の範囲ではＤ−グルコースの溶解速度が殆ど一定であることを見出した。従って、Ｄ−グルコースの溶解速度を測定する水又は水溶液のｐＨは５．５から９．５の範囲で計測することが好ましい。 When measuring the concentration of monomolecular D-glucose by the GOD method, it is necessary to know in advance how much the influence of pH is. The present inventor examined the pH dependence of the dissolution rate of monomolecular D-glucose using an aqueous solution adjusted to various pH by adding NaOH or hydrochloric acid to pure water. As a result, it was found that the dissolution rate of D-glucose was almost constant in the pH range of 5.5 to 9.5. Therefore, the pH of water or an aqueous solution for measuring the dissolution rate of D-glucose is preferably measured in the range of 5.5 to 9.5.
Ｄ−グルコース分子は、図１に示した構造式のものである。Ｄ−グルコースの水又は水溶液への溶解は、Ｄ−グルコース分子の両方の末端基が解離した水分子と作用して進行する。このため、単分子状Ｄ−グルコースの溶解速度を測定する際、水又は水溶液に添加するＤ−グルコースの量は、解離した水イオンの濃度を考慮して決めることが好ましい。即ち、添加するＤ−グルコースの量は、解離した水イオンの濃度により決まる単分子状Ｄ−グルコースの最大溶解濃度以下（最大計測範囲内）にすることが好ましい。例えば、最大計測範囲が６００ｍｇ／ｄｌの場合、測定対象水に添加するＤ−グルコースの量は３００ｍｇ／ｍｌ程度（最大溶解濃度の１／２程度）とすることが好ましい。 The D-glucose molecule has the structural formula shown in FIG. Dissolution of D-glucose in water or an aqueous solution proceeds by acting with water molecules in which both end groups of the D-glucose molecule are dissociated. For this reason, when measuring the dissolution rate of monomolecular D-glucose, the amount of D-glucose added to water or an aqueous solution is preferably determined in consideration of the concentration of dissociated water ions. That is, it is preferable that the amount of D-glucose to be added is not more than the maximum dissolution concentration of monomolecular D-glucose determined by the concentration of dissociated water ions (within the maximum measurement range). For example, when the maximum measurement range is 600 mg / dl, the amount of D-glucose added to the water to be measured is preferably about 300 mg / ml (about 1/2 of the maximum dissolution concentration).
また、水又は水溶液中に固体状Ｄ−グルコースを添加した後、Ｄ−グルコース分子の大半が単分子状で溶解するのに要する時間は、２５℃では７０分程度である。このため、Ｄ−グルコースの溶解速度計測は、Ｄ−グルコースを測定対象の水又は水溶液に添加してから６０分以内とすることが好ましい。 In addition, after adding solid D-glucose to water or an aqueous solution, the time required for most of the D-glucose molecules to dissolve in a monomolecular form is about 70 minutes at 25 ° C. For this reason, the dissolution rate measurement of D-glucose is preferably within 60 minutes after adding D-glucose to the water or aqueous solution to be measured.
例えば、電解生成水を測定対象の水又は水溶液に選び、この電解生成水１００ｍｌを２５℃に保ち、これに３００ｍｇ／ｄｌ以下の濃度となるようＤ−グルコースを添加し、添加後３０分以内にＧＤＯ法により単分子状Ｄ−グルコース濃度を計測する。同時に電解生成水のｐＨを測定しておく。 For example, electrolytically generated water is selected as water to be measured or an aqueous solution, 100 ml of electrolytically generated water is kept at 25 ° C., and D-glucose is added thereto so as to have a concentration of 300 mg / dl or less, and within 30 minutes after the addition. The monomolecular D-glucose concentration is measured by the GDO method. At the same time, the pH of electrolyzed water is measured.
一方、純水にＮａＯＨやＨＣｌを添加して電解することなく上記の電解生成水と同一のｐＨに調整した水溶液に対する単分子状Ｄ−グルコースの溶解速度を測定し、両者の溶解速度と比較することにより同一ｐＨでの電解生成水と非電解生成水の溶質溶解性を評価できる。 On the other hand, the dissolution rate of monomolecular D-glucose in an aqueous solution adjusted to the same pH as the electrolytically generated water is measured without adding NaOH or HCl to pure water and compared with the dissolution rate of both. Thus, the solute solubility of electrolyzed water and non-electrolyzed water at the same pH can be evaluated.
尚、本発明の溶解性表示方法では、測定対象の水又は水溶液に添加する溶質としてＤ−グルコースを用いる。Ｄ−グルコース以外の糖類として、果糖（単糖類）、乳糖（二糖類）、蔗糖（二糖類）及び澱粉（多糖類）がある。しかし、これらの糖類の水又は水溶液中濃度は酵素電極法では定量できないので、本発明の溶質としては採用できない。この他、Ｌ−アスコルビン酸については、酵素電極法での濃度測定が可能であるが、水又は水溶液への溶解速度が速すぎて本発明では採用できない。 In the solubility display method of the present invention, D-glucose is used as a solute added to the water or aqueous solution to be measured. Examples of sugars other than D-glucose include fructose (monosaccharide), lactose (disaccharide), sucrose (disaccharide), and starch (polysaccharide). However, the concentration of these saccharides in water or aqueous solution cannot be quantified by the enzyme electrode method and cannot be used as the solute of the present invention. In addition, L-ascorbic acid can be measured by the enzyme electrode method, but cannot be employed in the present invention because the dissolution rate in water or an aqueous solution is too fast.
次に、実施例により本発明を具体的に説明するが、本発明はこれに限定されるものではない。 Next, the present invention will be specifically described by way of examples, but the present invention is not limited thereto.
尚、単分子状Ｄ−グルコースの溶解速度は、下式により算出した。 The dissolution rate of monomolecular D-glucose was calculated by the following equation.
Ｙ＝（Ｄn−Ｄi）／θ
但し、Ｙは単分子状Ｄ−グルコース溶解速度（ｍｇ／ｄｌ・２０ｍｉｎ）、ＤiはＤ−グルコース添加前の測定対象の水又は水溶液中の単分子状Ｄ−グルコース濃度（ｍｇ／ｄｌ）、Ｄnは２０分経過後の測定対象の水又は水溶液中の単分子状Ｄ−グルコース濃度（ｍｇ／ｄｌ）、θはＤ−グルコース添加からの経過時間（２０ｍｉｎ）である。
Y = (Dn−Di) / θ
Where Y is the monomolecular D-glucose dissolution rate (mg / dl · 20 min), Di is the monomolecular D-glucose concentration (mg / dl) in the water or aqueous solution to be measured before the addition of D-glucose, Dn Is the monomolecular D-glucose concentration (mg / dl) in the water or aqueous solution to be measured after 20 minutes, and θ is the elapsed time (20 min) from the addition of D-glucose.
（実施例１）
導電率が０．３μＳ／ｃｍの純水を市販の連続電解式アルカリ電解水生成装置（株式会社リバーストーン社製）を用いて、電解強度レンジ１の設定にて流速毎分２リットルとしての電解を行い電解生成水を得た。この電解生成水のうち陰極側電解生成水を測定対象の水として、その溶解性を以下の通り評価した。
Example 1
Electrolysis with pure water having a conductivity of 0.3 μS / cm at a flow rate of 2 liters per minute at a setting of
先ず、得られた陰極側電解生成水（原水）を正確に１００ｍｌビーカーに採取し、ウォーターバス内にて２５℃に保持した。次いで、固体状（粉状）Ｄ−グルコース２５０ｍｇをビーカー内に添加し、測定を開始した。マグネティックスターラーとテフロン(登録商標)被覆回転子を用いてビーカー内溶液を撹拌した。約２０秒間撹拌した結果、透明なＤ−グルコース溶液（クラスター状で溶解）となった。引き続き、撹拌しながら２５℃にて１９分間保持した。更に、グルコカードダイアメーター（株式会社アークレイファクトリー社製）を用いてバイオセンサーをセットし、Ｄ−グルコースを電解生成水に加えてから１９分４０秒経過した時点で、このＤ−グルコース溶液をマイクロピペットにて１００μｌ採取し、スチロール樹脂板上に滴下した。更に、Ｄ−グルコース添加から２０分経過した時点で計測を開始し、単分子状Ｄ−グルコースの濃度を測定した。得られた単分子状Ｄ−グルコース濃度は２１４ｍｇ／ｄｌであった。尚、陰極側電解生成水（測定対象の水）のｐＨは８．１であった。これらの結果及び単分子状Ｄ−グルコース溶解速度の算出結果を表１に示す。 First, the obtained cathode side electrolysis generated water (raw water) was accurately collected in a 100 ml beaker and kept at 25 ° C. in a water bath. Next, 250 mg of solid (powdered) D-glucose was added into the beaker, and measurement was started. The solution in the beaker was agitated using a magnetic stirrer and a Teflon-coated rotor. As a result of stirring for about 20 seconds, a transparent D-glucose solution (dissolved in a cluster) was obtained. Subsequently, the mixture was kept at 25 ° C. for 19 minutes with stirring. Furthermore, when a biosensor is set using a gluco card diameter (manufactured by Arkray Factory Co., Ltd.) and D-glucose is added to the electrolyzed water, 19 minutes and 40 seconds elapses. 100 μl was collected with a pipette and dropped onto a styrene resin plate. Furthermore, measurement was started when 20 minutes had elapsed from the addition of D-glucose, and the concentration of monomolecular D-glucose was measured. The resulting monomolecular D-glucose concentration was 214 mg / dl. In addition, pH of cathode side electrolysis production | generation water (water of measurement object) was 8.1. These results and the calculation results of the monomolecular D-glucose dissolution rate are shown in Table 1.
（実施例２〜４）
連続電解式アルカリ電解水生成装置の電解強度設定をレンジ２〜４に変えて得られた陰極側電解生成水をそれぞれ測定対象の水とした以外は実施例１と同様に単分子状Ｄ−グルコースの濃度とｐＨを測定した。これらの結果及び単分子状Ｄ−グルコース溶解速度の算出結果を表１に示す。
(Examples 2 to 4)
Monomolecular D-glucose as in Example 1 except that the cathode-side electrolyzed water obtained by changing the electrolytic strength setting of the continuous electrolysis alkaline electrolyzed water generator to
（参考例１）
実施例１で用いた導電率が０．３μＳ／ｃｍの純水を電解することなくそのまま測定対象の水として用いた以外は実施例１と同様に単分子状Ｄ−グルコースの濃度とｐＨを測定した。これらの結果及び単分子状Ｄ−グルコース溶解速度の算出結果を表１に示す。
(Reference Example 1)
The concentration and pH of monomolecular D-glucose were measured in the same manner as in Example 1 except that the pure water having an electrical conductivity of 0.3 μS / cm used in Example 1 was used as the water to be measured without electrolysis. did. These results and the calculation results of the monomolecular D-glucose dissolution rate are shown in Table 1.
本発明においては、水又は水溶液中の水の解離度を表わす指標として表１の溶解速度で水又は水溶液の特性を表示するものである。 In the present invention, the characteristics of water or aqueous solution are displayed at the dissolution rate shown in Table 1 as an index representing the degree of dissociation of water in water or aqueous solution.
即ち、この指標で電解装置が製造する電解水がどの程度の解離度の電解水を製造できる能力を持っているかを定量的に表示することができる。 That is, it is possible to quantitatively display the degree of dissociation of the electrolyzed water produced by the electrolyzer using this index.
（参考例２〜３）
導電率が０．３μＳ／ｃｍの純水に試薬特級の希塩酸を加えてｐＨを５．６及び６．７に調節した塩酸水溶液を得た。これらの水溶液を測定対象の水として用いた以外は実施例１と同様に単分子状Ｄ−グルコースの濃度とｐＨとを測定した。これらの結果及び単分子状Ｄ−グルコース溶解速度の算出結果を表１に示す。
(Reference Examples 2-3)
An aqueous hydrochloric acid solution having pH adjusted to 5.6 and 6.7 by adding reagent-grade dilute hydrochloric acid to pure water having a conductivity of 0.3 μS / cm was obtained. The concentration and pH of monomolecular D-glucose were measured in the same manner as in Example 1 except that these aqueous solutions were used as measurement target water. These results and the calculation results of the monomolecular D-glucose dissolution rate are shown in Table 1.
（参考例４〜８）
導電率が０．３μＳ／ｃｍの純水に試薬特級のＮａＯＨ水溶液を加えてｐＨを８．３、９．４、１０．１、１０．６及び１１．１に調節した水酸化ナトリウム水溶液を得た。これらの水溶液を測定対象水として用いた以外は実施例１と同様に単分子状Ｄ−グルコースの濃度とｐＨを測定した。これらの結果及び単分子状Ｄ−グルコース溶解速度の算出結果を表１に示す。
(Reference Examples 4 to 8)
A sodium hydroxide aqueous solution whose pH is adjusted to 8.3, 9.4, 10.1, 10.6, and 11.1 by adding a reagent-grade NaOH aqueous solution to pure water having a conductivity of 0.3 μS / cm is obtained. It was. The concentration and pH of monomolecular D-glucose were measured in the same manner as in Example 1 except that these aqueous solutions were used as measurement target water. These results and the calculation results of the monomolecular D-glucose dissolution rate are shown in Table 1.
表１に示した実施例１から４と参考例１から８のｐＨ値（横軸）とＤ−グルコース濃度（縦軸）との相関図を図２に示す。図２中、Ｒａｎｇｅ １〜４（◆印で図示）はそれぞれ実施例１から４の結果を示す。また。参考例１〜８の結果を●印で示す。同一のｐＨで両者を比較すると（図２中の破線参照）陰極側電解生成水を用いた実施例のＤ−グルコース濃度は、何れも参考例のＤ−グルコース濃度よりも高濃度であり陰極側電解生成水の溶質溶解性が優れていることを的確に表示している。
FIG. 2 shows a correlation diagram between pH values (horizontal axis) and D-glucose concentrations (vertical axis) in Examples 1 to 4 and Reference Examples 1 to 8 shown in Table 1. In FIG. 2, Ranges 1 to 4 (shown by ♦) indicate the results of Examples 1 to 4, respectively. Also. The results of Reference Examples 1 to 8 are indicated by ●. When both were compared at the same pH (see the broken line in FIG. 2), the D-glucose concentration of the example using the cathode side electrolyzed water was higher than the D-glucose concentration of the reference example, and the cathode side It clearly indicates that the solute solubility of electrolyzed water is excellent.
（実施例５及び参考例９）
実施例２で得られた陰極側電解生成水（ｐＨ＝８．３）２００ｍｌに緑茶５ｇを加え、２５℃で２分間撹拌し、更に１分間静置し、生成水の色調を観察した。一方、参考例として、純水にＮａＯＨを加えてｐＨを８．３(上記陰極側電解生成水と同じ値）に調整した水酸化ナトリウム水溶液２００ｍｌに緑茶５ｇを加え、２５℃で２分間撹拌し、更に１分間静置し、水溶液の色調を比較観察した。その結果、陰極側電解生成水の方が水酸化ナトリウム水溶液よりも明確に濃厚な緑色を呈していることが観察された。この比較から、本発明の溶媒の溶解性表示方法によれば、水への緑茶成分の溶解速度を正確に評価できることが明らかである。
(Example 5 and Reference Example 9)
5 g of green tea was added to 200 ml of the cathode side electrolytically produced water (pH = 8.3) obtained in Example 2, stirred for 2 minutes at 25 ° C., and allowed to stand for 1 minute, and the color tone of the produced water was observed. On the other hand, as a reference example, 5 g of green tea was added to 200 ml of sodium hydroxide aqueous solution adjusted to pH 8.3 (same value as the above-mentioned cathode side electrolysis generated water) by adding NaOH to pure water, and stirred at 25 ° C. for 2 minutes. Further, the mixture was allowed to stand for 1 minute, and the color tone of the aqueous solution was comparatively observed. As a result, it was observed that the cathode-side electrolyzed water had a clearer green color than the sodium hydroxide aqueous solution. From this comparison, it is clear that according to the solvent solubility display method of the present invention, the dissolution rate of the green tea component in water can be accurately evaluated.
（参考例１０）
（ＧＯＤ法による糖類の検討）
Ｄ−グルコース、果糖（単糖類）、乳糖（二糖類）、蔗糖（二糖類）及び澱粉（多糖類）をそれぞれ２５０ｍｇ精秤し、各々を２５℃に保持した純水１００ｍｌに添加して溶解させた。添加してから７０分経過後に、各々の水溶液を採取し、それぞれの濃度をＧＯＤ法により計測した。その結果を表２に示す。
(Reference Example 10)
(Examination of saccharides by GOD method)
250 mg each of D-glucose, fructose (monosaccharide), lactose (disaccharide), sucrose (disaccharide) and starch (polysaccharide) are precisely weighed and added to 100 ml of pure water kept at 25 ° C. and dissolved. It was. 70 minutes after the addition, each aqueous solution was collected, and each concentration was measured by the GOD method. The results are shown in Table 2.
Claims (7)
The solubility display method according to claim 1, wherein the predetermined time is 1 to 60 minutes.
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