JP2006288261A - Method for producing tobanjan (fermented broad bean paste with red pepper) - Google Patents
Method for producing tobanjan (fermented broad bean paste with red pepper) Download PDFInfo
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Abstract
Description
本発明は、豆板醤の製造方法及び豆板醤を利用した食品に関するものである。より詳しくは、豆板醤の製造工程において、食塩濃度を調整し、かつ、バクテリオシンを利用することによって、芽胞菌による汚染度合いが少なく鮮やかな赤色でうま味の強い豆板醤の製造方法、該製造方法により製造された豆板醤、及び、該豆板醤を用いた食品に関するものである。 The present invention relates to a method for producing a bean plate soy and a food using the bean plate soy. More specifically, in the manufacturing process of bean plate soy, by adjusting the salt concentration and using bacteriocin, the method for manufacturing a bright red bean plate soy with a low degree of contamination by spore bacteria and a strong umami taste, the method It is related with the bean plate soy manufactured by this, and the foodstuff using this bean plate soy.
醤のルーツは中国であると言われている。古来では、大豆、そら豆を主原料とし、必要に応じて小麦粉を加えて天然発酵させて麹を作製していた。そして、この麹に塩水を加えたものを壺に入れ、その壷を室外において、日にさらし、夜露を浴びさせることにより発酵させて醤を製造してきた。
豆板醤（豆辣醤、豆辣ラージャン、そら豆ラージャーとも言われる。）は醤の1種類であり、大豆又はそら豆を主要原料として製造される。
Soy sauce is said to be in China. In ancient times, soybeans and broad beans were used as the main ingredients, and if necessary, wheat flour was added and fermented naturally to produce koji. And the thing which added salt water to this koji was put into a koji, and the koji was fermented by exposing it to the sun in the outdoors and exposing it to dew at night to produce soy.
Soy sauce (also known as soy bean sauce, soy bean rajan, broad bean rajah) is a kind of soy sauce and is produced using soybean or broad bean as the main ingredient.
豆板醤の製造においては、中国の四川省のピ県が有名である。このピ県産の豆板醤は、色が黒赤色で、辛味が濃いだけでなく、コク味も濃厚であり、非常に高品質の豆板醤とされている。発酵期間が長期にわたる程、濃厚なコク味が醸し出されるため味を重視する場合には、発酵期間が数年にもわたる豆板醤が使用されている。一方、味よりも鮮やかな赤色を重視する料理には、発酵期間が３〜６ヵ月程度の豆板醤が使用されている。しかしながら、いずれの豆板醤もうま味を補強する目的で、豆板醤にグルタミン酸を添加した多数のタイプでも流通している。 In the production of soy sauce, Pi County, Sichuan Province, China is famous. This bean plate soy produced in Pi County has a black-red color and not only has a strong pungent taste, but also a rich body, making it a very high quality bean plate soy sauce. If the emphasis is on the taste, the longer the fermentation period is, the richer the rich taste is, and soy sauce with a fermentation period of several years is used. On the other hand, bean plate soy with a fermentation period of about 3 to 6 months is used in dishes that emphasize bright red color rather than taste. However, for the purpose of reinforcing any bean plate soy flavor, many types of bean plate soy with glutamic acid added are also available.
このように豆板醤は、自然発酵法や高温短期間発酵法などいくつかの製造方法が検討されてきた。現在では、自然発酵法は四川省の一部で使用されているが、ほとんどは高温短期間発酵法が用いられている。 As described above, several methods for producing soy sauce have been studied, such as a natural fermentation method and a high-temperature short-term fermentation method. At present, the natural fermentation method is used in a part of Sichuan Province, but the high temperature short term fermentation method is mostly used.
自然発酵法は、一般に以下のような製造工程からなる。まず、蒸煮したそら豆に小麦粉を混ぜ合わせて、竹ザルに均一に薄めに乗せ、発酵室に置く。室温４０℃程度の発酵室で６〜７日間発酵させることにより、発酵室中の麹菌がそら豆の表面に菌糸を伸ばし、麹が生じる。麹を陶器製のかめに入れ、食塩水を加え、室外で４０〜５０日寝かした後、唐辛子と更に食塩を加え、均一に混合する。更に、室外で３〜５ヵ月貯蔵発酵させ豆板醤を製造する。しかしながら自然発酵法は、夏は４０℃以上、冬は氷点下となりうる、温度調節のされていない室外でかめを保存することに特徴があり、製品の品質が地域や気候に影響され、品質のバラツキが多いという問題点がある。 The natural fermentation method generally comprises the following production steps. First of all, mix steamed broad beans with flour, place it evenly on a bamboo colander and place it in the fermentation room. By fermenting in a fermentation room at a room temperature of about 40 ° C. for 6 to 7 days, koji molds in the fermentation room extend mycelia onto the surface of the beans, producing koji. Put the cocoon in a porcelain turtle, add salt water, sleep for 40-50 days outdoors, add chili and further salt, and mix evenly. Furthermore, it is stored and fermented outside for 3 to 5 months to produce bean plate soy. However, natural fermentation is characterized by preserving turtles outside the temperature-controlled outdoor environment, which can be above 40 ° C in the summer and below freezing in the winter. The quality of the product is affected by the region and climate, resulting in variations in quality. There is a problem that there are many.
高温短期間発酵法は、一般に以下のような製造工程からなる。まず、大豆あるいは脱皮そら豆を浸漬し、蒸煮し、冷却した後、麹菌を接種し、３０〜３８℃で数日間通風製麹することにより固体麹を作製する。固体麹に、磨砕した塩漬唐辛子、食塩水を加えて諸味を形成させる。該諸味を発酵槽で４０〜５５℃で１５〜３０日間発酵させて豆板醤を製造する。高温短期間発酵法は自然発酵法よりも貯蔵発酵時の平均温度が高いのが特徴である。このように高温で発酵させる製造方法は、発酵サイクルが短縮され設備利用率も向上させることができる為、量産化に適している。しかしながら、コク味といった官能の面では、伝統的な自然発酵法で製造された豆板醤には及ばないことが多い。 The high-temperature short-term fermentation method generally comprises the following production steps. First, soybeans or moulted beans are soaked, steamed and cooled, then inoculated with koji molds, and a solid koji is produced by ventilating at 30 to 38 ° C. for several days. Add crushed salted pepper and salt solution to the solid koji to form moromi. The moromi is fermented in a fermentor at 40 to 55 ° C. for 15 to 30 days to produce a bean plate soy. The high-temperature short-term fermentation method is characterized by a higher average temperature during storage fermentation than the natural fermentation method. Thus, the manufacturing method fermented at high temperature is suitable for mass production because the fermentation cycle can be shortened and the equipment utilization rate can be improved. However, in terms of sensuality such as richness, it is often not as good as the traditional soy sauce produced by natural fermentation.
ところで、自然発酵法の場合でも、高温短期間発酵法の場合でも静菌目的で食塩が添加されている。食塩は、麹菌の産生するプロテアーゼ、ペプチダーゼ活性を阻害するため、諸味の分解が進行しない原因となっている。その為、原料たんぱく質からのグルタミン酸の遊離が進まず、豆板醤のうま味が低い原因ともなっている。 By the way, sodium chloride is added for bacteriostatic purposes both in the case of the natural fermentation method and in the case of the high temperature short term fermentation method. Sodium chloride inhibits protease and peptidase activities produced by Aspergillus oryzae, and is a cause of the progress of mash taste degradation. For this reason, the release of glutamic acid from the raw protein does not progress, which is also the cause of the low umami taste of bean plate soy.
しかしながら、豆板醤の製造工程で食塩を添加しないと次のような点により、やはりグルタミン酸の遊離が進行しない。豆板醤の原料には穀類が用いられるが、穀類には芽胞菌が存在している。例えば、大豆にはバチルス・ズブチリス（Bacillus subtilis）が、そら豆にはバチルス・セレウス（Bacillus cereus）が生息していることが知られている。その為、大豆やそら豆等の穀類を原料に製麹した麹は、通常は芽胞菌に汚染されてしまう。しかし、麹に生息している芽胞菌は、諸味に添加される食塩によって芽胞を形成するため、増殖することができない。そのため豆板醤の品質にはほとんど影響しない。しかしながら、諸味の発酵熟成を無塩状態で行なうと芽胞菌が増殖しグルタミン酸の遊離を阻害する。更には、芽胞菌が増殖するに伴い、腐敗臭のような香気成分が諸味中に産出され、豆板醤の著しい品質低下を誘発する。 However, if sodium chloride is not added in the manufacturing process of bean plate soy, the release of glutamic acid does not proceed due to the following points. Cereals are used as the raw material for soy sauce, and spore bacteria are present in cereals. For example, it is known that Bacillus subtilis inhabits soybeans and Bacillus cereus inhabits soya beans. Therefore, koji made from cereals such as soybeans and broad beans is usually contaminated with spore bacteria. However, the spore bacteria living in the cocoons cannot grow because they form spores with the salt added to the moromi. Therefore, it hardly affects the quality of bean plate soy. However, if the ripening ripening of moromi is carried out in a salt-free state, the spore bacteria grow and inhibit the release of glutamic acid. Furthermore, as the spore bacteria grow, aroma components such as spoiled odor are produced in the moromi and induce a significant deterioration in the quality of the soy sauce.
また、前述したように自然発酵法或いは高温短期間発酵法などの従来法で豆板醤を製造する場合は、諸味の調製時に食塩を添加するため、芽胞菌は芽胞を形成し休眠状態で存在する。その結果、出来上がった豆板醤には多数の芽胞が存在している。ところで一般に加工食品は、豆板醤と比較して塩分濃度が低く、芽胞菌が増殖しやすい環境にある。そのため加工食品において豆板醤を使用する場合は、通常、芽胞を死滅させる条件で豆板醤を殺菌した後に使用されている。この殺菌工程で、豆板醤の品質が著しく低下してしまうため、豆板醤を使用した加工食品の品質も改善の余地があるものであった。 In addition, as described above, when producing bean plate soy by a conventional method such as a natural fermentation method or a high-temperature short-term fermentation method, salt is added during the preparation of moromi so that the spore bacteria form spores and exist in a dormant state. . As a result, a large number of spores are present in the soy sauce. By the way, processed foods are generally in an environment where the salinity is low compared to soy sauce and spore bacteria are likely to grow. Therefore, when using a bean plate soy in a processed food, it is usually used after sterilizing the bean plate soy under conditions that kill the spores. In this sterilization process, the quality of the bean plate soy is remarkably lowered, so the quality of the processed food using the bean plate soy also has room for improvement.
このような従来技術のもと、芽胞菌による汚染の度合いが少なく、鮮やかな赤色でかつうま味の強い豆板醤の製造方法が待ち望まれていた。 Under such a conventional technique, there has been a long-awaited method for producing a soy sauce with a bright red color and a strong umami taste.
ところで、１９９２年に食品の微生物制御の手段としてバイオプリザベーションを利用しようとする試みがB. Rayらによって提唱された。なかでも、バイオプリザバティブとしてバクテリオシンの利用が試みられている。例えば、ラクトコッカス（Lactococcus）属やラクトバチルス（Lactobacillus）属によって産生されるナイシン（クラスIに属するランチビオティック系のバクテリオシン）は、グラム陽性菌に対して幅広い静菌効果を有することが知られている（非特許文献１参照）。 By the way, in 1992, an attempt to use biopreservation as a means of controlling microorganisms in food was proposed by B. Ray et al. In particular, the use of bacteriocin as a biopreservative has been attempted. For example, nisin produced by the genus Lactococcus or Lactobacillus is known to have a broad bacteriostatic effect against gram-positive bacteria. (See Non-Patent Document 1).
グラム陽性菌に属する芽胞菌バチルス（Bacillus）属は、ナイシンによって殺菌されることが期待できるため、近年、ナイシンを麹の静菌剤として利用することが検討されつつある。例えば、バクテリオシンなどの乳酸醗酵液と水溶性カルシウム又は水溶性マグネシウムを麹の培養過程で添加する事を特徴とする、麹の雑菌阻止方法が知られている（特許文献１参照）。この方法では、速効性の殺菌効果を有する乳酸醗酵液と効力が持続する塩化カルシウム等を併用して、麹の雑菌の繁殖を阻止している。 Since the genus Bacillus belonging to the Gram-positive bacterium can be expected to be sterilized by nisin, in recent years, the use of nisin as a bacteriostatic agent is being studied. For example, a method for inhibiting miscellaneous bacteria of koji is known, characterized by adding a lactic acid fermentation broth such as bacteriocin and water-soluble calcium or water-soluble magnesium in the koji culture process (see Patent Document 1). In this method, a lactic acid fermentation broth having a fast-acting bactericidal effect is combined with calcium chloride or the like, which maintains its effectiveness, to inhibit the propagation of various germs.
しかしながら、この方法は、水溶性カルシウム又は水溶性マグネシウムが必須の構成要件であるため、水溶性カルシウム又は水溶性マグネシウムの添加量が多いと麹の風味に悪影響を与えてしまうという課題がある。また、当該発明は、酒、味噌、醤油等の中間原料とする麹を製造することを目的としており、蒸煮した大豆を磨砕した後に麹菌を添加した製造方法のみが開示されている。しかしながら、豆板醤の製法においてその様な工程をとると風味の点で好ましくなく豆板醤の製造に適したものではなかった。 However, in this method, since water-soluble calcium or water-soluble magnesium is an essential constituent, there is a problem that if the amount of water-soluble calcium or water-soluble magnesium added is large, the flavor of the koji is adversely affected. Moreover, the said invention aims at manufacturing the koji used as intermediate materials, such as liquor, miso, and soy sauce, and only the manufacturing method which added the koji mold after grinding the steamed soybean is disclosed. However, taking such a process in the method for producing soy sauce is not preferable in terms of flavor and is not suitable for producing soy sauce.
麹菌接種と発芽誘導物質の添加を行った後に、乳酸醗酵液等を添加して培養することを特徴とする麹の製造方法が知られている（特許文献２参照）。この方法では、乳酸醗酵液がバクテリオシンを含有しても良いとの記載がある。 A koji production method characterized by adding a lactic acid fermentation broth and culturing after koji mold inoculation and addition of a germination inducer is known (see Patent Document 2). In this method, there is a description that the lactic acid fermentation broth may contain bacteriocin.
しかしながら、この方法は、芽胞に対する発芽誘導物質であるアラニンを蒸煮後、３５℃程度に温度が低下した段階で、或いは種麹と共に添加し、時間差を保って乳酸醗酵液を添加することで、芽胞菌を減少させることに特徴があるものである。また、当該発明は、酒、味噌、醤油等の中間原料とする麹を製造することを目的としており、蒸煮した大豆を磨砕した後に麹菌を添加した製造方法のみが開示されている。しかしながら、豆板醤の製法においてその様な工程をとると風味の点で好ましくなく豆板醤の製造に適したものではなかった。更には、発芽誘導物質を添加して休眠状態の芽胞菌を出芽させ、増殖させる為、芽胞菌が産生する不必要な成分が豆板醤に含まれる結果となり望ましくなかった。 However, in this method, alanine, which is a germination inducer for spores, is cooked and then added at the stage where the temperature drops to about 35 ° C. or with seed meal, and the lactic acid fermentation liquor is added while keeping the time difference. It is characterized by reducing bacteria. Moreover, the said invention aims at manufacturing the koji used as intermediate materials, such as liquor, miso, and soy sauce, and only the manufacturing method which added the koji mold after grinding the steamed soybean is disclosed. However, taking such a process in the method for producing soy sauce is not preferable in terms of flavor and is not suitable for producing soy sauce. Furthermore, since a germination inducer is added to allow germination and growth of dormant spore bacteria, unnecessary components produced by the spore bacteria are included in the soy sauce, which is undesirable.
穀類を、ナイシン生産能を有する乳酸菌の存在下で水浸漬し、これを常法により加熱変性して得られる穀類を麹基質として用いることを特徴とする、微生物汚染の少ない麹の製造方法が知られている（特許文献３参照）。この方法は、麹の原料である穀類の汚染菌の生育抑制を行うことで、その後の汚染菌の生育も抑制している。 A method for producing koji with low microbial contamination is known, characterized in that cereals are dipped in water in the presence of lactic acid bacteria capable of producing nisin and heat-denatured by conventional methods and used as a koji substrate. (See Patent Document 3). This method also suppresses the growth of subsequent pollutants by suppressing the growth of cereal pollutants that are raw materials for straw.
しかしながら、この方法は、穀類の汚染菌の生育抑制を行うために、穀類を乳酸菌の存在下で、長時間（例えば１０〜５０時間）水浸漬する必要があり、量産化に不都合である。また、水浸漬温度が１５℃以下では、乳酸菌の増殖が緩慢となり、得られた穀類の汚染菌の生育抑制効果が得にくくなり、反対に、浸漬温度が３５℃以上では、穀類の成分が浸漬水に溶出し、原料利用率が低下すると同時に乳酸菌の生育も抑制される。そのため、この方法では温度管理も必要であるという課題があった。更には、１５〜３５℃という乳酸菌の至適温度かつ麹菌非存在下であらかじめ水浸漬するため、乳酸菌の過度の増殖を招き、製麹後の固体麹のｐＨが低くなるという欠点もあった。 However, this method is inconvenient for mass production because it is necessary to immerse the cereal in water in the presence of lactic acid bacteria for a long time (for example, 10 to 50 hours) in order to suppress the growth of cereal contaminating bacteria. On the other hand, when the water immersion temperature is 15 ° C. or lower, the growth of lactic acid bacteria becomes slow, and it becomes difficult to obtain the effect of suppressing the growth of contaminating bacteria of the obtained cereal. Conversely, when the immersion temperature is 35 ° C. or higher, the components of the cereal are immersed. It elutes in water, and the raw material utilization rate decreases, and at the same time, the growth of lactic acid bacteria is suppressed. Therefore, this method has a problem that temperature management is also necessary. Furthermore, since it is preliminarily immersed in water at an optimal temperature of lactic acid bacteria of 15 to 35 ° C. and in the absence of koji mold, excessive growth of the lactic acid bacteria is caused, and the pH of the solid koji after koji making becomes low.
米味噌あるいは豆味噌の製法において、その製造工程中にバチルス属細菌の汚染を受けるという課題を解決する方法として、発酵調味料の原料自体を乳酸醗酵させる、発酵調味料の製造方法が知られている（特許文献４参照）。 In the manufacturing method of rice miso or bean miso, as a method for solving the problem of being contaminated with bacteria belonging to the genus Bacillus during the production process, a method for producing a fermented seasoning is known in which the raw material of the fermented seasoning itself is lactic acid fermented (See Patent Document 4).
しかしながら、この方法は、バチルス属細菌の汚染を防ぐために、原料自体を乳酸醗酵させている。該発明には、乳酸醗酵に３日間及び２４時間かかる旨の開示があるように、乳酸醗酵の時間が必要であるという課題があり、量産化に不都合である。 However, in this method, the raw material itself is subjected to lactic acid fermentation in order to prevent contamination with Bacillus bacteria. In the invention, as disclosed in the fact that lactic acid fermentation takes 3 days and 24 hours, there is a problem that time for lactic acid fermentation is required, which is inconvenient for mass production.
このようにバクテリオシン等の食品への利用は、検討されているが、豆板醤の製造にバクテリオシンを利用することにより、豆板醤の芽胞菌汚染度合いを少なくし鮮やかな赤色でかつ、うま味を強くすることで豆板醤の品質を向上させる方法は知られていなかった。 In this way, the use of bacteriocin and other foods has been studied, but by using bacteriocin for the production of soy sauce, the degree of contamination of spore fungus in soy sauce is reduced, and it has a bright red and umami taste. There has been no known method for improving the quality of soy sauce by strengthening.
本発明は、芽胞菌の汚染度合いを少なくし、鮮やかな赤色でかつうま味の強い豆板醤の製造方法、該製造方法により製造された豆板醤、及び、該豆板醤を用いた食品を提供するものである。 The present invention provides a method for producing a vivid red and strong umami sauce, a bean plate soy produced by the production method, and a food using the bean plate soy, with less contamination of spore bacteria. It is.
本発明者らは、上記課題を解決するために検討した結果、豆板醤の製造工程中に、バクテリオシンを存在させ、かつ、除菌された空気を連続的又は簡潔供給しながら、手入れ時を除き密閉された状態の製麹機内で製麹し、得られた固体麹にバクテリオシンを添加した諸味の発酵熟成を食塩濃度が制御された状態で行なうことによって芽胞菌汚染が少なくうま味の強い豆板醤が製造できることを見出した。本発明は以下の内容を包含する。 As a result of studying to solve the above-mentioned problems, the inventors of the present invention have made bacteriocin present during the manufacturing process of bean plate soy, and continuously or concisely supplied sterilized air, while maintaining the maintenance time. A bean plate with less spore fungus contamination and a strong umami taste by mash fermentation in a sealed koji making machine and adding bacteriocin to the resulting solid koji in a state where the salt concentration is controlled. We found that soy can be produced. The present invention includes the following contents.
（１）麹菌を用いて固体麹を作成し、豆板醤を製造する工程において、１）穀類に麹菌及びバクテリオシンを添加し、２）除菌された空気を連続的又は間欠供給しながら、手入れ時を除き密閉された状態の製麹機内で製麹し、３）得られた固体麹に、バクテリオシンおよび固体麹重量の０〜５０倍重量の殺菌された食品素材を混合して諸味を形成し、４）次に、該諸味を食塩濃度が１２％以下に制御された状態で発酵熟成する一段階目の工程と、５）食塩濃度を０．１％以上に制御して発酵熟成する二段階目の工程を含み、６）一段階目の工程と二段階目の工程のいずれか若しくは両方の工程で唐辛子を添加することを特徴とする豆板醤の製造方法。（２）１段階目の発酵熟成前に固体麹を破砕する工程を含む請求項１記載の方法。（３）穀類が大豆及び／又はソラマメである請求項１ないし２記載の方法。（４）請求項１ないし３記載の方法で製造された豆板醤。（５）請求項４記載の豆板醤を用いた食品。（６）食品が、殺菌工程を経る加工食品であることを特徴とする請求項６記載の食品。 (1) In the process of producing solid koji using koji mold and producing soy sauce, 1) koji mold and bacteriocin are added to cereals, and 2) care is taken while supplying sterilized air continuously or intermittently. 3) Bacteriocin and sterilized food material of 0 to 50 times the weight of the solid koji are mixed with the obtained solid koji to form moromi. 4) Next, the first step of fermenting and ripening the moromi in a state where the salt concentration is controlled to 12% or less, and 5) Fermenting and aging by controlling the salt concentration to 0.1% or more 6) A method for producing soy sauce, characterized in that chili is added in either or both of the first step and the second step. (2) The method according to claim 1, comprising a step of crushing the solid koji before the first stage of fermentation ripening. (3) The method according to claim 1 or 2, wherein the cereal is soybean and / or broad bean. (4) Bean plate soy produced by the method according to any one of claims 1 to 3. (5) A food using the bean plate soy according to claim 4. (6) The food according to claim 6, wherein the food is a processed food that undergoes a sterilization process.
本発明の効果として、芽胞菌汚染が少なく鮮やかな赤色で、かつうま味の強い豆板醤の製造方法、該製造方法により製造された豆板醤、及び、該豆板醤を用いた食品を提供することが可能である。 As an effect of the present invention, there is provided a method for producing a soy bean sauce with a bright red, strong umami taste with little spore contamination, a soy sauce produced by the producing method, and a food using the soy sauce. Is possible.
以下に、本発明の実施の形態について説明する。 Embodiments of the present invention will be described below.
本発明において麹原料として使用する穀類は、通常の豆板醤の製造に用いられる原料を使用することができる。例えば、大豆、そら豆、小麦、米、麦などを用いることが出来る。必要により、これらの原料を水浸漬処理、蒸煮処理してもよい。穀類は１種類のみ使用してもよいし、２種類以上併用してもよい。豆板醤らしさを強めるため大豆又はそら豆を使用することが望ましい。さらに、麹菌の生育を促進するため小麦を併用することも望ましい。 In the present invention, the cereal used as the koji raw material can be a raw material used in the production of a normal soy sauce. For example, soybeans, broad beans, wheat, rice, wheat and the like can be used. If necessary, these raw materials may be subjected to water immersion treatment and steaming treatment. Only one type of cereal may be used, or two or more types may be used in combination. It is desirable to use soybeans or broad beans to enhance the soy bean soy. It is also desirable to use wheat in combination to promote the growth of Aspergillus.
上記原料に抗菌物質であるバクテリオシン及び麹菌を混合し、除菌された空気を供給でき、密閉できる製麹機に盛り込む。除菌された空気が供給でき、密閉できる製麹機とは、製麹機内に除菌された空気を供給する機能を持ち、製麹機内部と外気を遮断できる構造を持つものであり、例えば回転ドラム式製麹機を挙げることができる。また完全に密閉できなくても外気からの微生物の混入を防止できれば良い。構造がシンプルで安価な製麹機として、除菌された空気を製麹機内部に供給する構造を持ち、密閉状態が得られる開閉可能な蓋付の製麹機も例示することができる。空気の除菌方法は０．３μｍ以上の塵を９９．９７％以上集塵できるフィルター、例えばＨＥＰＡフィルターなどを用いることができる。尚、製麹機の排風口には外気を遮断するためのフィルターを取り付けることが好ましい。密閉できない製麹機、例えば円盤回転式製麹機、静地通風式製麹機などは、外気からの微生物の混入を免れず、品質の低下を招くことがある。通風の方式は特に限定されるものではなく、内部通風方式、表面通風方式などを用いることができる。 Bacteriocin and bacilli, which are antibacterial substances, are mixed with the above raw materials, and then sterilized air can be supplied and incorporated into a sealing machine that can be sealed. Sterile iron making machine that can supply sterilized air and can be sealed has a function of supplying sterilized air inside the iron making machine and has a structure that can block the inside of the iron making machine from outside air, for example A rotating drum type iron making machine can be mentioned. Even if it cannot be completely sealed, it is only necessary to prevent the entry of microorganisms from the outside air. As a simple and inexpensive iron making machine, there can be exemplified an iron making machine with an openable / closable lid having a structure for supplying sterilized air to the inside of the iron making machine. As a method for sterilizing air, a filter that can collect 99.97% or more of dust of 0.3 μm or more, such as a HEPA filter, can be used. In addition, it is preferable to attach the filter for interrupting | blocking outside air to the exhaust port of a iron making machine. A steel making machine that cannot be sealed, such as a disk-rotating type steel making machine, a static ground ventilation type steel making machine, etc., is unavoidable of contamination of microorganisms from the outside air and may cause a deterioration in quality. The ventilation method is not particularly limited, and an internal ventilation method, a surface ventilation method, or the like can be used.
本発明において、バクテリオシンを添加するとはバクテリオシン含有物を添加するだけでなく、バクテリオシン生産菌を添加することでバクテリオシンを存在させることも含まれる。バクテリオシン生産菌をあらかじめ最適条件で培養し、バクテリオシンを含有する培養物として添加することが操作性の観点から望ましい。バクテリオシン種類は、豆板醤の製造工程において汚染菌である芽胞菌を殺菌し、豆板醤が製造可能である限りにおいて特に制限されない。そのようなバクテリオシンとしてグラム陽性菌に作用するバクテリオシンを上げることができる。グラム陽性菌に作用するバクテリオシンとしては、ナイシン、ラクティシン、ペディオシン、サカシン、ヌカシンなどがあり、中でも、ナイシンは世界各国で食品添加物として認可されていることから市場の受容性から考えても好ましい。なお、ナイシンのようにナイシンＡ、ナイシンＺ、ナイシンＱなど類縁体を有するものもあるが、芽胞菌を殺菌できる限りにおいていずれでもかまわない。バクテリオシン生産菌としては、ラクトコッカス・ラクティス（Lactococcus lactis）、ペディオコッカス・ペントーサス（Pediococcus pentosaceus）、ラクトバチルス・サケイ（Lacotobacillus sakei）、ラクトバチルス・アシドフィラス（Lactobacillus acidophilus）等の乳酸菌、ビフィドバクテリウム・ビフィダム（Bifidobacterium bifidum）等のビフィズス菌、プロピオニバクテリウム・ソエニ（Propionibacterium thoenii）等のプロピオン酸菌をあげることができる。バクテリオシンの培養方法が広く確立されている乳酸菌を使用することが望ましい。バクテリオシンの至適存在量は、その種類によって異なるが、固体麹作製時又は固体麹作成時及び諸味作製時に存在し、芽胞菌を殺菌できればよい。その存在量が多いほど微生物の混入や増殖を防ぐことができるため、効果の観点から存在量が多ければ多いほど望ましいことは言うまでもない。例えば、ナイシンの場合は、固体麹作製時、望ましくは固体麹作製時及び諸味作製時に存在していればよい。具体的には、そら豆１gあたり１IU以上、望ましくは10IU以上、より望ましくは100IU以上、更に望ましくは1,000IU以上存在していればよい。上限は特にないが、100,000IU以下、望ましくは10,000IU以下に制限することが製造コストの観点から望ましい。 In the present invention, adding bacteriocin includes not only adding a bacteriocin-containing material but also adding bacteriocin to the presence of bacteriocin. It is desirable from the viewpoint of operability that the bacteriocin-producing bacteria are cultured in advance under optimum conditions and added as a culture containing bacteriocin. The bacteriocin type is not particularly limited as long as it can sterilize spore bacteria which are contaminating bacteria in the manufacturing process of bean plate soy and can manufacture bean plate soy. As such bacteriocin, bacteriocin acting on Gram-positive bacteria can be raised. As bacteriocin acting on Gram-positive bacteria, there are nisin, lactisin, pediocin, sakacin, nukacin, etc. Among them, nisin is preferred in view of market acceptance because it is approved as a food additive in various countries around the world. . Some of them have analogs such as nisin A, nisin Z, and nisin Q, such as nisin, but any of them may be used as long as spore bacteria can be sterilized. Examples of bacteriocin-producing bacteria include Lactococcus lactis, Pediococcus pentosaceus, Lactobacillus sakei, Lactobacillus acidophilus and other lactic acid bacteria, Bifidobacteria Examples thereof include bifidobacteria such as Bifidobacterium bifidum and propionic acid bacteria such as Propionibacterium thoenii. It is desirable to use lactic acid bacteria for which bacteriocin culture methods are widely established. The optimum amount of bacteriocin varies depending on the type of the bacteriocin, but it may be present when the solid koji is produced, or when the solid koji is produced and when the moromi is produced, and it is sufficient that the spore bacteria can be sterilized. It is needless to say that the larger the abundance is, the more desirable the abundance is from the viewpoint of the effect, since the larger the abundance can prevent the contamination and proliferation of microorganisms. For example, in the case of nisin, it may be present at the time of solid koji preparation, desirably at the time of solid koji preparation and moromi preparation. Specifically, it may be present at 1 IU or more, preferably 10 IU or more, more preferably 100 IU or more, more preferably 1,000 IU or more per 1 g of beans. Although there is no particular upper limit, it is desirable from the viewpoint of manufacturing cost to limit to 100,000 IU or less, preferably 10,000 IU or less.
本発明において使用する麹菌は、通常の豆板醤の製造に用いられるものであればとくに制限されない。一例として、アスペルギルス・オリゼ（Aspergillus oryzae）, アスペルギルス・ソヤ（Aspergillus sojae）,アスペルギルス・ニドランス（Aspergillus nidulans）,アスペルギルス・ニガー（Aspergillus niger）,アスペルギルス・アワモリ（Aspergillus awamori）等のアスペルギルス（Aspergillus）属、リゾパス・オリゼ（Rhizopus oryzae）、リゾパス・オリゴスポラス（Rhizopus oligosporus）等のリゾパス（Rizopsu）属、モナスカス・アンカ（Monascus anka）, モナスカス・プルプレウス（Monascus purpureus）等のモナスカス（Monascus）属を挙げることができる。優良菌株の育種例の多い、アスペルギルス・オリゼ（Aspergillus oryaze）やアスペルギルス・ソヤ（Aspergillus sojae）が汎用性が高く望ましい。 The koji mold used in the present invention is not particularly limited as long as it is used for the production of a normal bean plate soy. As an example, Aspergillus oryzae, Aspergillus sojae, Aspergillus nidulans, Aspergillus niger, Aspergillus awamori, Aspergillus gillus, etc. Rhizopus oryzae, Rhizopus oligosporus and other genus Rizopsu, Monascus anka, Monascus purpureus and other monascus genus . Aspergillus oryaze and Aspergillus sojae, which have many examples of breeding excellent strains, are highly versatile and desirable.
本発明において、製麹とは、穀類とバクテリオシン、及び麹菌の混合物を製麹機内に盛り込んだ後、手入れ（麹菌の生育を促進するために、製麹機内に盛り込まれた混合物を例えば１０時間毎に１５分程度攪拌する工程）の際を除いて、製麹機内を密閉した状態で一定の温度に制御して培養することを意味する。制御温度は豆板醤が製造できる条件である限り特に制限されないが、２５〜４０℃、望ましくは２８〜３７℃、より望ましくは２９〜３４℃の温度に制御して培養することにより高品質の豆板醤を製造することができる。温度が低すぎると麹菌の増殖度合いが悪く、穀類が十分に分解されず豆板醤の強いうま味が得られず好ましくない。一方、温度が高すぎると原料蛋白質の分解に必要な酵素活性が低くなり強いうま味が得られず好ましくない。なお、一定の温度に制御するとは、その温度幅で管理することを意味し、麹菌の発熱等によって一時的に設定温度幅を逸脱してもよい。逸脱した場合は、加熱・冷却することによって一定の設定温度幅になるように培養管理されていればよい。培養期間は、通常の場合と同様に菌糸が穀物原料を覆い固体麹が製麹される期間とすることができる。使用する麹菌、使用する製麹装置等によって異なるため、通常は経験者が製麹中の固体麹を指でさわり水分量などを判断して製麹期間を決定している。一例として、１５〜２４０時間、望ましくは２０〜１２０時間、より望ましくは３０〜７２時間、３５〜６０時間と設定することができる。培養期間が短すぎると、麹菌が十分に増殖できず、豆板醤の深いコク味が得られない。培養期間が長すぎると、汚染菌を制御するために必要なバクテリオシン量が増大し、コスト増を招きやはり望ましくない。 In the present invention, koji refers to a mixture of cereals, bacteriocin, and koji molds in a koji making machine, and then care (for example, a mixture of koji molds contained in the koji making machine is promoted for 10 hours. Except for the step of stirring for about 15 minutes every time), it means that the culture is controlled at a constant temperature while the inside of the iron making machine is sealed. The controlled temperature is not particularly limited as long as it is a condition capable of producing soy bean soy, but it is cultured at a temperature of 25 to 40 ° C., preferably 28 to 37 ° C., more preferably 29 to 34 ° C. Soy sauce can be manufactured. If the temperature is too low, the degree of growth of Aspergillus is poor, and the cereals are not sufficiently decomposed, and the strong umami taste of bean plate soy is not obtained. On the other hand, if the temperature is too high, the enzyme activity required for the decomposition of the raw material protein is lowered, and a strong umami taste cannot be obtained, which is not preferable. Controlling to a constant temperature means that the temperature is managed by the temperature range, and the set temperature range may be temporarily deviated by heat generation of the koji mold. When deviating, it is only necessary that the culture is controlled so that a certain set temperature range is obtained by heating and cooling. The culture period can be a period in which the mycelium covers the grain raw material and the solid koji is made as in the normal case. Since it varies depending on the koji mold to be used, the koji making apparatus to be used, etc., the experienced person usually touches the solid koji in the koji making with his / her finger to determine the moisture content and the like and determines the koji making period. As an example, it can be set as 15 to 240 hours, desirably 20 to 120 hours, more desirably 30 to 72 hours, 35 to 60 hours. If the culture period is too short, the koji mold cannot be sufficiently grown and the deep-boiled taste of bean plate soy cannot be obtained. If the culture period is too long, the amount of bacteriocin necessary for controlling the contaminating bacteria increases, which causes an increase in cost and is also undesirable.
次に得られた固体麹とバクテリオシンを混合して諸味を形成し、食塩濃度が制御された状態で発酵熟成する。なお、諸味の作製の前後で固体麹を物理的に破砕してもよい。破砕度合いは、固体麹が軽くつぶれる状態でもよいし、ペースト状になるまで行なってもよい。破砕することによって発酵熟成する期間を短縮することができるため、製造コストの観点から望ましい。また、諸味を形成するとき固体麹重量の０〜５０倍重量の殺菌された食品素材を混合して諸味を形成してもよい。混合する食品素材は、食品の製造に使用できるものであり、諸味中の食塩濃度を１２重量％以下に調整できる限りにおいて、特に制限されない。大豆、そら豆、麦、小麦、米等の穀類、唐辛子などを混合しても良い。これら食品素材を殺菌する方法は、汚染微生物を殺菌できる限り特に制限されないが、蒸煮や塩蔵が風味の観点から好ましい。諸味形成時に加える重量の下限は、ゼロすなわち加えないことも含む。また、重量の上限は、固体麹重量の５０倍重量まで、望ましくは１０倍重量まで、より望ましくは５倍重量まで、さらに望ましくは３倍重量までである。この値を超えると諸味の１段階目発酵熟成が進行しにくい為、好ましくない。 Next, the resulting solid koji and bacteriocin are mixed to form moromi, and fermented and matured with the salt concentration controlled. The solid cake may be physically crushed before and after the production of moromi. The degree of crushing may be a state in which the solid koji is lightly crushed or may be performed until it becomes a paste. Since the period for fermentation and ripening can be shortened by crushing, it is desirable from the viewpoint of production cost. Moreover, when forming moromi, you may form moromi by mixing the sterilized food raw material of 0-50 times the weight of solid soot. The food material to be mixed is not particularly limited as long as it can be used for the production of food and the salt concentration in moromi can be adjusted to 12% by weight or less. You may mix soybeans, broad beans, wheat, wheat, rice grains, and chili peppers. The method for sterilizing these food materials is not particularly limited as long as contaminating microorganisms can be sterilized, but steaming and salting are preferred from the viewpoint of flavor. The lower limit of the weight added at the time of moromi formation includes zero, that is, no addition. The upper limit of the weight is 50 times the weight of the solid soot weight, desirably 10 times the weight, more desirably 5 times the weight, and even more desirably 3 times the weight. If this value is exceeded, the first stage fermentation ripening of the moromi is difficult to proceed, which is not preferable.
諸味の食塩濃度の制御は、固体麹とバクテリオシンを混合する際に添加する食塩水或いは食塩量を調節することによって実施することができる。諸味の発酵熟成中の塩分濃度は１２％以下、望ましくは８％以下、より望ましくは５％以下、更に望ましくは２％以下に制御することが必要である。塩分濃度が高すぎると麹菌のプロテアーゼ、ペプチダーゼの活性を阻害し、原料蛋白質の分解が進まず、豆板醤のうま味が強くならず好ましくない。 The salt concentration of moromi can be controlled by adjusting the amount of salt solution or salt added when mixing the solid koji and bacteriocin. It is necessary to control the salt concentration during fermentation and ripening of moromi to 12% or less, desirably 8% or less, more desirably 5% or less, and even more desirably 2% or less. If the salt concentration is too high, the activity of the koji mold protease and peptidase is inhibited, the decomposition of the raw material protein does not proceed, and the umami of the soy sauce is not preferred.
豆板醤中のグルタミン酸含有量を上昇させる目的の一段階目発酵熟成は２０〜５０℃、好ましくは２０〜４５℃、よりこのましくは２５〜３７℃に保温し、１〜５０日間、好ましくは３〜３０日間、より好ましくは４〜１４日間、更に好ましくは４〜９日間行なう。温度が２０℃未満では蛋白質の分解が十分でなく、強いうま味が得られない。一方、５０℃を越える温度では諸味に含まれる糖とアミノ酸がメイラード反応により褐変して苦味が発現しやすく、官能的に好ましくない傾向にある。日数についても同様の傾向があり、２４時間未満であれば蛋白質の分解が十分でなく強いうま味が得られにくい。また、５０日間以上では、二段階目発酵熟成の期間に影響を与えやすい。 The first stage fermentation ripening for the purpose of increasing the glutamic acid content in the bean plate soy is kept at 20-50 ° C., preferably 20-45 ° C., more preferably 25-37 ° C., for 1-50 days, preferably The treatment is performed for 3 to 30 days, more preferably 4 to 14 days, and further preferably 4 to 9 days. If the temperature is less than 20 ° C., the protein is not sufficiently decomposed and a strong umami taste cannot be obtained. On the other hand, at temperatures exceeding 50 ° C., sugars and amino acids contained in moromi taste brown due to the Maillard reaction and tend to develop bitterness, which tends to be undesirably functional. There is a similar tendency with respect to the number of days. If it is less than 24 hours, the protein is not sufficiently decomposed and it is difficult to obtain a strong umami taste. Moreover, in 50 days or more, it is easy to influence the period of the second stage fermentation ripening.
豆板醤の味をまとめ風味を改善する目的の二段階目発酵熟成では、通常、唐辛子を添加する。第一段階目発酵熟成で唐辛子を添加しなかった場合は、必ず本工程で唐辛子を添加する必要がある。本工程における食塩濃度の制御は、食塩水、食塩、或いは塩蔵唐辛子を添加することなどによって実施することができる。諸味中の塩分濃度は０．１重量％以上、好ましくは１重量％以上、より好ましくは３重量％以上、より好ましくは５重量％以上に制御する必要がある。塩分濃度が低すぎると、唐辛子の味にまとまりがなく好ましくない。上限は特にないが、２５重量％以下に制御することが望ましい。塩分濃度が高すぎると食塩が大幅に析出してしまい、操作性に影響を与える。なお、第一段階目発酵熟成ですでに唐辛子を添加しており、かつ食塩濃度が０．１重量％以上の場合は、１段階目発酵に引き続いてそのまま２段階目発酵熟成を行っても良い。 In the second stage fermentation ripening for the purpose of summarizing the taste of bean sauce and improving the flavor, chili is usually added. When chili is not added in the first stage fermentation ripening, it is necessary to add chili in this process. The control of the salt concentration in this step can be carried out by adding saline, salt, or salted pepper. The salt concentration in the moromi must be controlled to 0.1 wt% or more, preferably 1 wt% or more, more preferably 3 wt% or more, more preferably 5 wt% or more. If the salt concentration is too low, the taste of the chili pepper is not preferred, which is not preferable. Although there is no upper limit in particular, it is desirable to control to 25 weight% or less. If the salt concentration is too high, the salt will largely precipitate, affecting operability. In addition, when chili has already been added in the first stage fermentation ripening and the salt concentration is 0.1% by weight or more, the second stage fermentation ripening may be performed as it is after the first stage fermentation. .
豆板醤の味をまとめる目的で行う２段階目発酵熟成は、２０〜５０℃、好ましくは２０〜４５℃、よりこのましくは２５〜３７℃に保温し、１〜１８０日間、好ましくは３〜１２０日間、より好ましくは７〜９０日間行う。温度が低すぎると味がまとまらず、高すぎるとアミノ酸と糖が反応しすぎて褐変臭が強まる。一方、２段階目発酵熟成期間が短すぎると味がまとまらず好ましくない。期間が長すぎると、アミノ酸と糖が反応しすぎて褐変臭がして望ましくない。 The second stage fermentation ripening performed for the purpose of summarizing the bean plate soy is kept at 20 to 50 ° C., preferably 20 to 45 ° C., more preferably 25 to 37 ° C., for 1 to 180 days, preferably 3 to 120 days, more preferably 7 to 90 days. If the temperature is too low, the taste will not be settled. If it is too high, the amino acid and sugar will react too much and the browning odor will increase. On the other hand, if the second stage fermentation ripening period is too short, the taste is not settled, which is not preferable. If the period is too long, the amino acid and the sugar react too much to cause a brown odor, which is undesirable.
本発明において、固体麹に添加混合する唐辛子に限定はなく、一例として、生唐辛子、塩漬された唐辛子、乾燥唐辛子、唐辛子粉末等が挙げられる。また辣醤とは、唐辛子の発酵物または調味唐辛子である。 In the present invention, the chili added to and mixed with the solid koji is not limited, and examples include raw chili, salted chili, dried chili, chili powder, and the like. The soy sauce is a fermented or seasoned chili pepper.
本発明の方法で製造された豆板醤は、うま味が非常に強く、さらに芽胞菌が大幅に少ないため、過度に殺菌する必要がなく、広く加工食品に使用することができる。 The bean plate soy produced by the method of the present invention has a very strong umami taste, and further has few spore bacteria. Therefore, it does not need to be sterilized excessively and can be widely used for processed foods.
本発明において、豆板醤を用いた食品とは特に限定はなく、通常の豆板醤と同様に使用することができる。各種炒め物、各種揚げ物、或いは各種あわせ調味料に使用することができる。調理中に加熱工程を経る料理では、豆板醤の風味がより際だち好ましい。具体例の一部として、麻婆豆腐、麻婆春雨、魚香茄子、麻婆茄子、回鍋肉、干焼蝦仁、豆板醤入り炒り卵などを挙げることができる。 In this invention, there is no limitation in particular with the foodstuff using a bean plate soy, It can use similarly to a normal bean plate soy. It can be used for various stir-fried foods, various fried foods, or various seasonings. In cooking that undergoes a heating step during cooking, the flavor of the bean plate soy is more particularly preferred. Examples of specific examples include mapo tofu, maple vermicelli, fish scented eggplant, maple egg, casserole meat, dried sardines, fried egg with soy sauce.
本発明において、豆板醤を用いた食品の製造工程において殺菌工程を有するとは、食品の品質を維持する為に汚染微生物を死滅させる工程を有することを意味する。原料としての豆板醤をあらかじめ殺菌してから使用してもよいし、豆板醤はあらかじめ殺菌せず出来上がった食品を殺菌してもよい。或いは、両方を併用してもよい。殺菌方法は、汚染微生物を死滅させる限りにおいて制限されないが、６０℃で３０分、７０℃で３０分、８０℃で１０分、９０℃で１０分等が用いられる。殺菌工程は、目的の品質を達成できる条件であればよい。例えば、レトルト食品では、水分活性０．９４以上、ｐＨ４．６以上の食品では１２０℃４分の加熱殺菌条件などである。 In this invention, having a sterilization process in the manufacturing process of the foodstuff using a bean plate soy means having a process of killing a contaminating microorganism in order to maintain the quality of a foodstuff. The bean plate soy as a raw material may be used after being sterilized in advance, or the bean plate soy may be sterilized in advance without sterilizing it. Or you may use both together. The sterilization method is not limited as long as the contaminating microorganism is killed, but 60 ° C. for 30 minutes, 70 ° C. for 30 minutes, 80 ° C. for 10 minutes, 90 ° C. for 10 minutes, and the like are used. The sterilization process should just be the conditions which can achieve the target quality. For example, for retort food, the water sterilization condition is 120 ° C. for 4 minutes for a food having a water activity of 0.94 or more and a pH of 4.6 or more.
（実施例）
以下に、実施例に基づいて本発明を詳細に説明するが、本発明はこれらにより何ら限定されるものではない。
(Example)
Hereinafter, the present invention will be described in detail based on examples, but the present invention is not limited thereto.
（実施例１）
（バクテリオシン粉末の調製）
ナイシンＺを含有するラクティスエイドＤＬ７溶液（オーム乳業製、ナイシン活性7,000ＩＵ／ｍｌ）４８０Ｌを常法に従い、ＣＰスケールＵＦモジュール（ＳＡＰ−３０１３：分画分子量4,000Ｄａ）を用い、２５Ｌまで濃縮し、次にＢＰスケールＵＦモジュール（ＳＡＰ−１０１３：分画分子量4,000Ｄａ）を用い、１５Ｌまで濃縮した後、凍結乾燥することにより、260万ＩＵ／ｇの抗菌活性を有するナイシンＺ粉末を調製した。ナイシンＺ活性は、ＨＰＬＣによる定量及びバイオアッセイ（Appl. Microbiol. Biotechnol. (1996) 45:36-40 A. Ishizaki et al.）により調べた。
Example 1
(Preparation of bacteriocin powder)
480 L of lactis aid DL7 solution containing nisin Z (Ohm Dairies, Nisin activity 7,000 IU / ml) was concentrated to 25 L using a CP scale UF module (SAP-3013: molecular weight cut off 4,000 Da) according to a conventional method, Next, nisin Z powder having antibacterial activity of 2.6 million IU / g was prepared by concentrating to 15 L using a BP scale UF module (SAP-1013: molecular weight cut off 4,000 Da) and then freeze-drying. Nisin Z activity was determined by HPLC quantification and bioassay (Appl. Microbiol. Biotechnol. (1996) 45: 36-40 A. Ishizaki et al.).
（バクテリオシン乳酸菌培養液の調製）
バクテリオシン乳酸菌培養液は、ラクトコッカス・ラクティス（Lactococcus lactis） AJ110212 (FERM BP-8552)株Tioglycolate without glucose培地（Difco製）にて３７℃で２４時間培養し、その培養液を５０ｍLのＹＤ培地（０．５％酵母エキス、０．５％塩化ナトリウム、３．０％グルコース、１．５％炭酸カルシウム、ｐＨ ７．０に調製、坂口フラスコ）にシードして１００ｒｐｍで振とうし、約20時間のバッチ培養を行った（ナイシン活性約11,000IU/ml）。まれに培養ロットによっては、ナイシン活性が11,000IU/mlにならない場合もあるが、その時は実施例１で調製したナイシンを添加することによってナイシン活性を11,000IU/mlになるように調製した。
(Preparation of bacteriocin lactic acid bacteria culture solution)
The culture solution of bacteriocin lactic acid bacteria was cultured for 24 hours at 37 ° C. in Tioglycolate without glucose medium (Lifococcus lactis) AJ110212 (FERM BP-8552) strain Tioglycolate without glucose medium (manufactured by Difco). 0.5% yeast extract, 0.5% sodium chloride, 3.0% glucose, 1.5% calcium carbonate, adjusted to pH 7.0, seeded into Sakaguchi flask) and shaken at 100 rpm for about 20 hours (Nisin activity about 11,000 IU / ml). In rare cases, the nisin activity may not be 11,000 IU / ml depending on the culture lot. At that time, the nisin activity was adjusted to 11,000 IU / ml by adding nisin prepared in Example 1.
（乳酸菌体の調製）
ナイシン非生産菌であるラクトバチルス・サケイ（Lactobacillus sakei）AJ3158をTioglycolate without glucose培地（Difco製）にて３７℃で２４時間培養し、その培養液を５０ｍLのＹＤ培地（０．５％酵母エキス、０．５％塩化ナトリウム、３．０％グルコース、１．５％炭酸カルシウム、ｐＨ ７．０、坂口フラスコ）にシードして１００ｒｐｍで振とうし、約20時間のバッチ培養を行った。遠心分離により、乳酸菌体を回収し、上清を除去した。乳酸菌体を生理食塩水（食塩濃度0.9%）で洗浄し、再度遠心分離により乳酸菌体を集菌して、乳酸菌体を調製した。
(Preparation of lactic acid bacteria)
A nisin non-producing bacterium, Lactobacillus sakei AJ3158 was cultured in Tioglycolate without glucose medium (manufactured by Difco) for 24 hours at 37 ° C., and the culture was mixed with 50 mL of YD medium (0.5% yeast extract, 0.5% sodium chloride, 3.0% glucose, 1.5% calcium carbonate, pH 7.0, Sakaguchi flask) and shaken at 100 rpm to perform batch culture for about 20 hours. The lactic acid bacteria were recovered by centrifugation and the supernatant was removed. The lactic acid bacteria were washed with physiological saline (salt concentration: 0.9%), and again collected by centrifugation to prepare lactic acid bacteria.
（製麹時バクテリオシン添加効果の検証）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液或いは滅菌水１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、うま味を評価した。評価は直接、２つの対象品をなめることにより比較する２点嗜好法によって行なった。
その結果、２０名中１９名が製麹時に実施例２で調製した培養液（ナイシン含有）を添加した豆板醤のうま味が勝っていると評価した（参考：優位水準０．１％以上、統計的官能検査法（佐藤信著、日科技連出版社、１９８５年初版、ISDN4-8171-9011-6、３１５頁）。
(Verification of bacteriocin addition effect at the time of iron making)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution or sterilized water prepared in Example 2 and 0.1 g of seed candy (manufactured by Bioc, bean miso seed potato) were placed in a sterilized plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. After this pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a panel of 20 experts. The evaluation was made directly by a two-point preference method that compares two items by licking.
As a result, 19 out of 20 people evaluated that the umami of bean plate soy added with the culture solution (containing nisin) prepared in Example 2 at the time of koji was superior (reference: superiority level 0.1% or more, statistics Sensory test method (Nobu Sato, Nikka Giren Publisher, 1985, first edition, ISDN 4-8171-9011-6, pages 315).
（除菌された空気の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合し、密閉されるラボ製麹機に盛込み、３０℃、９０％ＲＨにて４３時間製麹した。製麹中の発酵熱の除熱には、フィルターを通して除菌した空気を用い製麹中は、手入れの際を除いて、密閉された状態の製麹機内で製麹した。或いは、製麹中の発熱の除熱に、外気を用いて行い（ラボ製麹機のフィルターをはずして外気を供給した。）、手入れは製麹開始後１０時間毎に２分間行った。各々得られた固体麹８００ｇに、実施例２で調製した培養液を３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加えて混合し、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。その結果、２０名中２０名が除菌した空気を用いた豆板醤のうま味が勝っていると評価した（優位水準０．１％以上）。
(Examination of sterilized air)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed candy (Bioc, Miso seed potato) are placed in a sterilized plastic bag and mixed well. Smelting was performed at 30 ° C. and 90% RH for 43 hours. For removal of the fermentation heat during the koji making, air sterilized through a filter was used, and during the koji making, the koji was made in a hermetically sealed koji making machine except during the care. Alternatively, the outside air was used to remove heat generated during the iron making (the outside air was supplied after removing the filter of the laboratory iron making machine), and the maintenance was carried out for 2 minutes every 10 hours after the start of the iron making. To each 800 g of the solid koji obtained, 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc, yeast for strong miso) were added and mixed, and a chopper (Nippon Carrier Kogyo Co., Ltd., Golden Crusher and paste were made by chopper GM-D). This paste was filled in a laminated pouch so as to be 500 g per bag. After this pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people. As a result, it was evaluated that the umami taste of bean plate soy using air sterilized by 20 of 20 people was superior (dominance level of 0.1% or more).
（１段階目発酵熟成における食塩濃度の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ、及び食塩２０ｇ（食塩濃度約１．８％）或いは２００ｇ（食塩濃度約１５．４％）加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填し、このパウチを３０℃、７日間保温し１段階目発酵熟成を行った。固体麹あたりの唐辛子量及び食塩量を同じくらいにするために、１段階目発酵熟成時に食塩を２０ｇ添加した実験区には、諸味全量に滅菌唐辛子を１９２０ｇ、食塩を１８０ｇ加え混合し、１段階目発酵熟成時に食塩を２００ｇ添加した実験区には、諸味全量に滅菌唐辛子を１９２０ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤中の可溶化窒素あたりの遊離グルタミン酸量を測定したところ、食塩２０ｇ添加した実験区では０．６５％、食塩２００ｇ添加した実験区では０．２２％であった。更に、出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。
その結果、２０名中２０名が１段階目発酵熟成時に食塩を２０ｇ添加した実験区、すなわち食塩量を制御して１段階目発酵熟成を行なった豆板醤のうま味が勝っていると評価した（優位水準０．１％以上）。
(Examination of salt concentration in the first stage fermentation aging)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. To 800 g of the obtained solid koji, 300 ml of the culture solution prepared in Example 2, 1.92 ml of yeast (Bioc, yeast for strong miso), and 20 g of sodium chloride (salt concentration of about 1.8%) or 200 g (salt concentration) About 15.4%) After addition and mixing, the mixture was pulverized and pasted with a chopper (Golden Chopper GM-D, manufactured by Nippon Carrier Kogyo Co., Ltd.). This paste was filled in a laminated pouch so as to be 500 g per bag, and this pouch was kept at 30 ° C. for 7 days to perform first stage fermentation aging. To equalize the amount of pepper and salt per solid koji, add 20g of salt during the first stage fermentation and maturation. Add 1920g of sterilized pepper and 180g of salt to the total amount of moromi and mix. In the experimental group to which 200 g of sodium chloride was added at the time of eye fermentation aging, 1920 g of sterilized chili was added to and mixed with the entire amount of moromi. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
When the amount of free glutamic acid per solubilized nitrogen in the soy bean soy was measured, it was 0.65% in the experimental group to which 20 g of sodium chloride was added and 0.22% in the experimental group to which 200 g of sodium chloride was added. Furthermore, the bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized, and sensory evaluation of the bean plate soy was performed. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people.
As a result, 20 people out of 20 evaluated that the umami of bean plate soy that had been subjected to the first stage fermentation ripening by controlling the amount of salt and the experiment group in which 20 g of sodium chloride was added during the first stage fermentation ripening was evaluated. Superiority level 0.1% or more).
（２段階目発酵熟成における食塩濃度の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌを加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに1袋につき５００ｇとなるように充填し、このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し滅菌した唐辛子を１２００ｇ（食塩濃度約０％）、或いは滅菌した唐辛子を１２００ｇと食塩を５０ｇ（食塩濃度約２．８％）混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。
その結果、２０名中２０名が２段階目発酵熟成時に食塩を添加した豆板醤のうま味が勝っていると評価した（優位水準０．１％以上）。
また、豆板醤中の食塩濃度をあわせる目的で２段階目発酵熟成時に食塩を添加しなかった実験区に食塩を５０ｇ添加して同様に官能評価を実施したが、やはり２０名中２０名が２段階目発酵熟成時に食塩を添加した豆板醤のうま味が勝っており、更に味のまとまりがよく好ましいと評価した（優位水準０．１％以上）。
(Examination of salt concentration in the second stage fermentation ripening)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. To 800 g of the obtained solid koji, 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc, yeast for strong miso) were added and mixed, and then a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper). It was pulverized and pasted by GM-D). This paste is filled into a laminated pouch so as to be 500 g per bag, this pouch is kept at 30 ° C. for 7 days and subjected to the first stage fermentation and aging, and then 1200 g of sterilized chili pepper against 500 g of the pouch (about a salt concentration of about 0%), or 1200 g of sterilized chili and 50 g of sodium chloride (salt concentration of about 2.8%) were mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people.
As a result, 20 people out of 20 evaluated that the umami taste of bean plate soy added with salt during the second stage fermentation aging was superior (over 0.1% superiority level).
In addition, 50 g of sodium chloride was added to the experimental section where no salt was added during the second stage fermentation ripening for the purpose of adjusting the salt concentration in the soy sauce soy sauce. It was evaluated that the umami of bean plate soy added with salt at the stage of fermentation and ripening was superior, and that the unity of taste was better and favorable (over 0.1% superiority level).
（製麹温度の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、９０％ＲＨにして４３時間製麹した（二葉科学社製、恒温恒室槽）。製麹温度は２０〜４０℃の各条件を検討した。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し2段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、直なめによりうま味を評価した。評価は５点満点で行い、優位水準１％以上で３点以上となる条件を好ましい温度とした。
判断基準：５点・・・非常に強いうま味を感じ、官能的にも好ましい
４点・・・強いうま味を感じ、官能的にも好ましい
３点・・・うま味を感じ、官能的にも好ましい
２点・・・うま味を感じるが官能的に異風味を感じる
１点・・・うま味をあまり感じないがコントロールよりはうま味を感じる
０点・・・コントロールとして、実施例４でバクテリオシン乳酸菌培養無添加（ナイシン無添加）で製麹した豆板醤を用いた。
(Investigation of iron making temperature)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a straw lid and made into 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). The iron making temperature was examined under each condition of 20 to 40 ° C. Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. After this pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation ripening was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. An expert panel consisting of 20 people evaluated the umami taste in a straightforward manner. The evaluation was performed with a maximum score of 5 points, and a condition where the superiority level was 1% or more and 3 or more points was set as a preferable temperature.
Judgment criteria: 5 points ... feels a very strong umami taste and is also sensually favorable
4 points ... Feels strong umami and is sensually preferable
3 points ... feels umami and is sensually desirable
2 points ... feels umami but sensually feels a different flavor
1 point ... I don't feel much umami but I feel more umami than control
0 point: As a control, bean plate soy prepared in Example 4 without addition of bacteriocin lactic acid bacteria culture (no addition of nisin) was used.
その結果、製麹温度条件は、２０〜４０℃でコントロールより好ましいことが判明したが、２５〜３５℃の範囲でより好ましいことが判明した。 As a result, it was found that the ironmaking temperature condition is more preferable than the control at 20 to 40 ° C., but more preferable in the range of 25 to 35 ° C.
（２段階目発酵熟成温度の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにして４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに1袋につき５００ｇとなるように充填した。このパウチを２０〜５０℃の各条件で７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例８と同様に直なめによりうま味を評価した。
(Examination of the second stage fermentation aging temperature)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a straw lid, and the mixture was made at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Science Co., Ltd., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. The pouch was incubated for 7 days at 20 to 50 ° C. and subjected to the first stage fermentation and maturation, and then 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 8 by a professional panel consisting of 20 people.
その結果、１段階目発酵熟成温度は、２０〜５０℃でコントロールよりも好ましいことが判明した。 As a result, it was found that the first stage fermentation ripening temperature is 20 to 50 ° C., which is preferable to the control.
（１段階目発酵熟成期間の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにして４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。1番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに1袋につき５００ｇとなるように充填した。このパウチを３０℃で３〜６０日間保温し1段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例８と同様に直なめによりうま味を評価した。
(Examination of the first stage fermentation aging period)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a straw lid, and the mixture was made at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Science Co., Ltd., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After the first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. The pouch was kept at 30 ° C. for 3 to 60 days and subjected to the first stage fermentation and maturation, and then 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 8 by a professional panel consisting of 20 people.
その結果、１段階目発酵熟成期間は３日の段階でコントロールより好ましいことが判明したが、４〜５０日間でより好ましいことがわかった。 As a result, the first stage fermentation ripening period was found to be preferable to the control at the stage of 3 days, but it was found to be more preferable at 4 to 50 days.
（２段階目発酵熟成温度の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにして４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃で７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を２０〜５０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例８と同様に直なめによりうま味を評価した。
(Examination of the second stage fermentation aging temperature)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a straw lid, and the mixture was made at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Science Co., Ltd., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. The pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed. Then, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 20-50 ° C. for 30 days, and the second stage fermentation ripening was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 8 by a professional panel consisting of 20 people.
その結果、２段階目発酵熟成温度は、２０〜５０℃でコントロールよりも好ましいことが判明し、かつ２５〜４５℃でより好ましいことが判明した。 As a result, the second stage fermentation ripening temperature was found to be more preferable than the control at 20 to 50 ° C., and more preferable at 25 to 45 ° C.
（２段階目発酵熟成期間の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにして４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに1袋につき５００ｇとなるように充填した。このパウチを３０℃で７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、１〜１８０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例８と同様に直なめによりうま味を評価した。
(Examination of the second stage fermentation aging period)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a straw lid, and the mixture was made at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Science Co., Ltd., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized into paste. This paste was filled in a laminated pouch so as to be 500 g per bag. The pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed. Then, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 1 to 180 days, and the second stage fermentation ripening was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 8 by a professional panel consisting of 20 people.
その結果、１段階目発酵熟成期間は１日の段階でコントロールより好ましいことが判明したが、３〜１８０日間でより好ましいことがわかった。 As a result, the first stage fermentation ripening period was found to be preferable to the control at the stage of 1 day, but it was found to be more preferable for 3 to 180 days.
（第１段階目発酵と第２段階目発酵の統合検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹を用いて以下の２つの条件を検討した。
条件１：得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ、食塩３５ｇ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填し、このパウチを３０℃、７日間保温し１段階目発酵熟成を行った。諸味全量に滅菌唐辛子を１９２０ｇ、食塩を１８０ｇ加え混合し、この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。（１段階目発酵熟成：食塩濃度約３．０％、２段階目発酵熟成：食塩濃度約１．１％）
条件２：得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ、食塩６０ｇ（食塩濃度約１．８％）、及び滅菌唐辛子１９２０ｇを加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填し、このパウチを３０℃、７日間保温し１段階目発酵熟成を行った。そのまま引き続き、この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。（１段階目発酵熟成：食塩濃度約１．１％、２段階目発酵熟成：食塩濃度約１．１％）
得られた豆板醤の可溶化窒素あたりの遊離グルタミン酸含有量を測定したところ条件１は０．６５％、条件２は０．７２％であった。いずれも、実施例１９に記載の四川豆板醤の値を上回っていた。
(Integration study of the first stage fermentation and the second stage fermentation)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. The following two conditions were examined using the obtained solid soot.
Condition 1: 300 ml of the culture broth prepared in Example 2, 1.92 ml of yeast (Bioc, yeast for strong miso) and 35 g of sodium chloride were added to and mixed with 800 g of the obtained solid koji, followed by chopper (Nihon Carrier Industry Co., Ltd.) The product was pulverized and pasted with a golden chopper GM-D manufactured by Komatsu. This paste was filled in a laminated pouch so as to be 500 g per bag, and this pouch was kept at 30 ° C. for 7 days to perform first stage fermentation aging. 1920 g of sterilized pepper and 180 g of sodium chloride were added to and mixed with the entire amount of moromi, and the moromi was kept at 30 ° C. for 30 days, followed by second-stage fermentation ripening. (First stage fermentation ripening: salt concentration about 3.0%, second stage fermentation ripening: salt concentration about 1.1%)
Condition 2: To 800 g of the obtained solid koji, 300 ml of the culture solution prepared in Example 2, 1.92 ml of yeast (Bioc, yeast for strong miso), 60 g of sodium chloride (salt concentration of about 1.8%), and sterilization After adding 1920 g of chili pepper and mixing, it was pulverized and pasted with a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM-D). This paste was filled in a laminated pouch so as to be 500 g per bag, and this pouch was kept at 30 ° C. for 7 days to perform first stage fermentation aging. The moromi was kept warm at 30 ° C. for 30 days, and the second stage fermentation ripening was performed. (First stage fermentation ripening: salt concentration about 1.1%, second stage fermentation ripening: salt concentration about 1.1%)
When the content of free glutamic acid per solubilized nitrogen of the obtained soy sauce was measured, the condition 1 was 0.65% and the condition 2 was 0.72%. All exceeded the value of Sichuan bean plate soy described in Example 19.
（ナイシン添加量の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、ナイシン乳酸菌培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。なお本検討で用いたナイシン乳酸菌培養液は、実施例３で調製した菌体を用いて菌体濃度がソラマメ１ｇあたり１０８cfuになるように調製し、実施例１で調製したナイシン粉末を溶解させることによりナイシン濃度がソラマメ１ｇあたり０、１０、１００、１，０００或いは１０，０００IUとなるように調製した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ、塩蔵唐辛子を１９２０ｇ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行い、そのまま続けて３０℃、３０日間保温する２段階目発酵熟成を行なった。
発酵熟成後の豆板醤中の芽胞菌量を測定したところ、製麹時にナイシン濃度無添加区では芽胞菌が１０５コ／ｇ検出されたが、その他の条件では検出されなかった。このことより、製麹時にナイシンが必要であることがわかった。
(Examination of nisin addition amount)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of nisin lactic acid bacteria culture solution, and 0.1 g of seed pod (manufactured by Bioc Co., Ltd., bean miso seed pod) were placed in a sterilized plastic bag and mixed well. The nisin lactic acid bacteria culture solution used in this study was prepared using the cells prepared in Example 3 so that the cell concentration was 10 8 cfu per 1 g of broad bean, and the nisin powder prepared in Example 1 was dissolved. The nisin concentration was adjusted to 0, 10, 100, 1,000, or 10,000 IU per gram of broad bean. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. 800 g of the obtained solid koji was mixed with 300 ml of the culture solution prepared in Example 2, 1.92 ml of yeast (Bioc, yeast for strong miso), and 1920 g of salted pepper, and then mixed with a chopper (Nippon Carrier Kogyo Co., Ltd.). Made by Golden Chopper GM-D) and made into a paste. This paste was filled in a laminated pouch so as to be 500 g per bag. The pouch was kept at 30 ° C. for 7 days and subjected to the first stage fermentation and aging, and then the second stage fermentation and aging was carried out as it was kept at 30 ° C. for 30 days.
When the amount of spore bacteria in the soybean plate soy after fermentation and maturation was measured, 10 5 bacteria / g of spore bacteria were detected in the nisin non-addition zone at the time of koji making, but were not detected under other conditions. From this, it was found that nisin is necessary at the time of ironmaking.
（乾燥ソラマメでの検討）
乾燥ソラマメ１ｋｇに４Ｌの水を添加し、１晩水で浸漬した後、蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液或いは滅菌水１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。
その結果、２０名中１９名が製麹時にナイシンを添加した豆板醤のうま味が勝っていると評価した（優位水準０．１％以上）。このことから乾燥ソラマメを用いた検討でも、好ましい結果が得られることが判明した。
(Examination with dried broad beans)
4 L of water was added to 1 kg of dried broad beans, soaked in water overnight, and then steamed at 121 ° C. for 5 minutes in a steam kettle. 1 kg of steamed broad bean, 10 ml of the culture solution or sterilized water prepared in Example 2 and 0.1 g of seed candy (manufactured by Bioc, bean miso seed potato) were placed in a sterilized plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. After this pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people.
As a result, 19 people out of 20 evaluated that the umami taste of bean plate soy added with nisin at the time of koji was superior (over 0.1% superiority level). From this, it was found that favorable results were obtained even in the study using dried broad beans.
（大豆での検討）
大豆１ｋｇに６００ｇの水を添加し、室温で３時間吸水させた後、蒸気釜にて１１４℃、４０分蒸煮した。蒸煮大豆１ｋｇ、実施例２で調製した培養液或いは滅菌水１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。
その結果、２０名中１９名が製麹時にナイシンを添加した豆板醤のうま味が勝っていると評価した（優位水準０．１％以上）。このことから大豆を用いた検討でも、好ましい結果が得られることが判明した。
(Examination with soybean)
After adding 600 g of water to 1 kg of soybeans and absorbing water at room temperature for 3 hours, it was steamed in a steam kettle at 114 ° C. for 40 minutes. 1 kg of steamed soybeans, 10 ml of the culture solution or sterilized water prepared in Example 2 and 0.1 g of seed candy (Bioc Co., Ltd., bean miso seed potato) were placed in a sterilized plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. After this pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people.
As a result, 19 people out of 20 evaluated that the umami taste of bean plate soy added with nisin at the time of koji was superior (over 0.1% superiority level). From this, it was found that favorable results were obtained even in the study using soybean.
（大豆、ソラマメ混合状態での検討）
皮むきしたソラマメ０．５ｋｇを蒸気釜にて１２１℃、５分蒸煮した。また、大豆０．５ｋｇに３００ｇの水を添加し、室温にて３時間吸水させた後、蒸気釜にて１１４℃、４０分蒸煮した。蒸煮ソラマメ、蒸煮大豆各０．５ｋｇ、実施例２で調製した培養液或いは滅菌水１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し１段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し2段階目発酵熟成を行なった。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。
その結果、２０名中１９名が製麹時にナイシンを添加した豆板醤のうま味が勝っていると評価した（優位水準０．１％以上）。このことから大豆、そら豆を用いた検討でも、好ましい結果が得られることが判明した。
(Examination with soybean and broad bean mixed condition)
0.5 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. In addition, 300 g of water was added to 0.5 kg of soybeans, and water was absorbed at room temperature for 3 hours, and then steamed at 114 ° C. for 40 minutes in a steam kettle. Steamed faba bean, steamed soybean 0.5 kg each, the culture solution prepared in Example 2 or 10 ml of sterilized water, and 0.1 g of seed meal (BIOCK, bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized into paste. This paste was filled in a laminated pouch so as to be 500 g per bag. After this pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation ripening was performed.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people.
As a result, 19 people out of 20 evaluated that the umami taste of bean plate soy added with nisin at the time of koji was superior (over 0.1% superiority level). From this, it was found that favorable results could be obtained even in studies using soybeans and broad beans.
（唐辛子の種類の検討）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液或いは滅菌水を１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ、及び食塩２０ｇ（食塩濃度約１．８％）加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填し、このパウチを３０℃、７日間保温し１段階目発酵熟成を行った。諸味全量に滅菌唐辛子を１９２０ｇ、食塩を１８０ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。滅菌唐辛子は牛角型唐辛子又は羊角型唐辛子又は天鷹椒又は朝天椒を用いた。
出来上がった豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、実施例４と同様に直なめによりうま味を評価した。
その結果、製麹時にナイシンを添加した豆板醤のうま味が勝っていた（２点嗜好法で評価。牛角型唐辛子の場合：２０名中１９名が製麹時に実施例２で調製した培養液（ナイシン含有）を添加した豆板醤を好ましいと評価した。優位水準０．１％以上。羊角型唐辛子の場合：２０名中１８名が製麹時に実施例２で調製した培養液（ナイシン含有）を添加した豆板醤を好ましいと評価した。優位水準０．１％以上。天鷹椒の場合：２０名中１７名が製麹時に実施例２で調製した培養液（ナイシン含有）を添加した豆板醤を好ましいと評価した。優位水準１％以上。朝天椒の場合：２０名中１８名が製麹時に実施例２で調製した培養液（ナイシン含有）を添加した豆板醤を好ましいと評価した。優位水準０．１％以上）。
この結果より、特に唐辛子の種類によらず製麹時ナイシン等のバクテリオシン添加の効果があることがわかった。
(Examination of the kind of chili pepper)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution or sterilized water prepared in Example 2, and 0.1 g of soy sauce (manufactured by Bioc Co., Ltd., bean miso soy sauce) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. To 800 g of the obtained solid koji, 300 ml of the culture solution prepared in Example 2, 1.92 ml of yeast (Bioc, yeast for strong miso), and 20 g of sodium chloride (salt concentration of about 1.8%) were added and mixed. The mixture was pulverized and pasted with a chopper (Golden Chopper GM-D, manufactured by Nippon Carrier Kogyo Co., Ltd.). This paste was filled in a laminated pouch so as to be 500 g per bag, and this pouch was kept at 30 ° C. for 7 days to perform first stage fermentation aging. 1920 g of sterilized chili and 180 g of sodium chloride were added to the total amount of moromi and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed. As the sterilized chili, beef horn-type chili, ram horn-shaped chili, Tentaka cocoon, or Asa tempura was used.
The finished bean plate soy was kept in an 80 ° C. water bath for 13 minutes, sterilized and subjected to sensory evaluation of the bean plate soy. Umami was evaluated by a straightening as in Example 4 by a professional panel consisting of 20 people.
As a result, the umami of soy sauce with nisin added during koji making was superior (evaluated by the two-point preference method. In the case of beef horn-type chili: 19 out of 20 culture solutions prepared in Example 2 during koji making ( The soy sauce with added nisin was evaluated to be preferable, and the superiority level was 0.1% or more.In the case of ergot-shaped chili: 18 out of 20 people prepared the culture solution (containing nisin) prepared in Example 2 at the time of koji making. It was evaluated that the added soy sauce was preferable.The superiority level was 0.1% or more.In the case of Tentaka candy: 17 people in 20 people added the culture solution (containing nisin) prepared in Example 2 at the time of koji making. Superiority level of 1% or more In the case of Chaotian rice cake: 18 out of 20 people evaluated that bean plate soy added with the culture solution (containing nisin) prepared in Example 2 at the time of koji making was preferred. Level 0.1% or more).
From this result, it was found that there is an effect of adding bacteriocin such as nisin at the time of making the koji regardless of the kind of chili.
（四川豆板醤との比較）
皮むきしたソラマメ１ｋｇを蒸気釜にて１２１℃、５分蒸煮した。蒸煮ソラマメ１ｋｇ、実施例２で調製した培養液１０ｍｌ、種麹（ビオック社製、豆味噌用種麹）０．１ｇを滅菌ビニール袋にいれてよく混合した。混合物を麹フタに移し、３０℃、９０％ＲＨにて４３時間製麹した（二葉科学社製、恒温恒室槽）。手入れは製麹開始後１７時間後、２４時間後、３８時間後に行った。１番手入れ後に麹フタを少しあけ水分が蒸発するようにして製麹した。得られた固体麹８００ｇに、実施例２で調製した培養液３００ｍｌ、酵母（ビオック社製、強力味噌用酵母）１．９２ｍｌ加え混合した後、チョッパー（株式会社日本キャリア工業社製、ゴールデンチョッパーＧＭ−Ｄ）により粉砕、ペースト状とした。このペーストをラミネートパウチに１袋につき５００ｇとなるように充填した。このパウチを３０℃、７日間保温し1段階目発酵熟成を行った後、パウチ５００ｇに対し塩蔵牛角唐辛子を１２００ｇ加え混合した。この諸味を３０℃、３０日間保温し２段階目発酵熟成を行なった。
出来上がった試作豆板醤、購入した半年もの四川豆板醤（商品タイプ名：ピーシェントーバン、ブランド名：娟城 豆瓣醤。四川省ピ県豆板股分有限公司製。本発明において「半年もの豆板醤」は、特に限定がなければこの豆板醤を意味する）、及び購入した３年もの豆板醤（商品タイプ名：ピーシェントーバン、ブランド名：娟城 豆瓣醤。四川省ピ県豆板股分有限公司製。本発明において「３年もの豆板醤」は、特に限定がなければこの豆板醤を意味する）の理化学分析を行った。その結果を表６に示す。
(Comparison with Sichuan bean plate soy)
1 kg of peeled broad bean was steamed in a steam kettle at 121 ° C. for 5 minutes. 1 kg of steamed broad bean, 10 ml of the culture solution prepared in Example 2 and 0.1 g of seed meal (Bioco., Bean miso seed meal) were placed in a sterile plastic bag and mixed well. The mixture was transferred to a lid, and was smelted at 30 ° C. and 90% RH for 43 hours (manufactured by Futaba Kagaku Co., constant temperature and constant temperature chamber). Care was carried out 17 hours, 24 hours and 38 hours after the start of the ironmaking. After first cleaning, the lid was slightly opened to make the water so that the water evaporated. After adding 300 ml of the culture solution prepared in Example 2 and 1.92 ml of yeast (Bioc yeast, yeast for strong miso) to 800 g of the obtained solid koji, and mixing them, a chopper (manufactured by Nippon Carrier Kogyo Co., Ltd., Golden Chopper GM). -D) pulverized and pasted. This paste was filled in a laminated pouch so as to be 500 g per bag. The pouch was kept at 30 ° C. for 7 days and first stage fermentation ripening was performed. Then, 1200 g of salted beef horn pepper was added to 500 g of the pouch and mixed. This moromi was kept at 30 ° C. for 30 days, and the second stage fermentation aging was performed.
Prototype soy bean soy, half-year Sichuan bean soy purchased (product type name: peasant toban, brand name: Sojo bean soy sauce. Made in Sichuan Pi bean crotch co., Ltd. , Unless otherwise specified, means this bean plate soy sauce), and purchased three-year bean plate soy (product type name: peasent toban, brand name: 娟 chen bean soy sauce. In the invention, “3-year soy sauce” means this soy sauce unless otherwise specified). The results are shown in Table 6.
その結果は、色度は、試作豆板醤がもっとも赤色に近かった。また、半年もの豆板醤及び３年もの豆板醤からは芽胞菌が検出されたが、試作豆板醤からは検出されなかった。
また、試作豆板醤を８０℃ウオーターバスに１３分間保温し、殺菌して豆板醤の官能評価を行った。２０名からなる専門パネラーにより、直なめによりうま味を評価した。
評価基準：５点・・・非常に強いうま味を感じ、官能的にも好ましい。
４点・・・強いうま味を感じ、官能的にも好ましい
３点・・・うま味を感じ、官能的にも好ましい
２点・・・うま味を感じるが官能的に異風味を感じる
１点・・・うま味をあまり感じない
その結果を表７に示す。
As a result, the chromaticity of the prototype soy sauce was the closest to red. In addition, spore bacteria were detected from half-year soy sauce and three-year soy sauce, but not from prototype soy sauce.
In addition, the prototype soy sauce was kept in an 80 ° C. water bath for 13 minutes, sterilized, and sensory evaluation of the soy sauce was performed. An expert panel consisting of 20 people evaluated the umami taste in a straightforward manner.
Evaluation criteria: 5 points: A very strong umami taste is felt, which is also preferable in terms of sensuality.
4 points ... Feels strong umami and is sensually preferable
3 points ... feels umami and is sensually desirable
2 points ... feels umami but sensually feels a different flavor
1 point ... not so much umami taste The results are shown in Table 7.
その結果、試作豆板醤のうま味が最も強いことがわかった。 As a result, it was found that the umami taste of the prototype soy sauce was strongest.
（メニュー系での評価 〜麻婆豆腐）
実施例１９で作製した試作豆板醤、半年もの豆板醤、及び３年もの豆板醤を用いて、麻婆豆腐を試作した。レシピ：材料 豆腐４００ｇ、豚ひき肉８０ｇ、長ネギ３０ｇ、しょうが１５ｇ、にんにく３ｇ、豆板醤１０ｇ、醤油大さじ１、砂糖小さじ１、酒小さじ１、鶏がらスープ１５０ｇ、油大さじ１、片栗粉大さじ１（水６５ｇで溶く）、山椒適量。作り方：しょうが、にんにく、長ネギはあらかじめみじん切りにした。豆腐は２センチ角の賽の目に切った。フライパンに油を入れ、中火で加熱し、ひき肉を軽く炒め、弱火にして、しょうが、にんにく、豆板醤を入れさらに炒めた。スープ、調味料（醤油、砂糖、酒）を加えた後、豆腐を加え、弱火で煮た。豆腐に火が通ったら長ねぎを入れ、水溶き片栗粉を入れ一煮立ちさせ、とろみをつけた。
豆板醤は可溶化窒素が同じになる分量添加した。また、メニューに添加する食塩量を調整して、いずれの豆板醤を用いた場合でもメニュー中の塩分濃度が同一になるように調整した。
出来上がった、麻婆豆腐のうま味を２０名の専門パネラーにて実施例１９と同様に評価した。その結果を表８に示す。
(Evaluation in the menu system-Mapo tofu)
Mapo tofu was made using the prototype soy sauce prepared in Example 19, half-year soy sauce, and three-year soy sauce. Recipe: Tofu 400g, ground pork 80g, green onion 30g, ginger 15g, garlic 3g, soy sauce 10g, 1 tablespoon soy sauce, 1 teaspoon sugar, 1 teaspoon sake, 150g chicken soup, 1 tablespoon oil, 1 tablespoon starch ), The appropriate amount of yam. How to make: Ginger, garlic and long onion were chopped in advance. The tofu was cut into 2 cm squares. Put the oil in a frying pan, heat it over medium heat, stir the minced meat lightly, set it to low heat, add ginger, garlic, bean plate soy sauce and further fry. After adding soup and seasonings (soy sauce, sugar, sake), tofu was added and simmered on low heat. When the tofu was cooked, the green onions were put in it, and the water-soluble potato starch was put in it and boiled.
Soy sauce was added in an amount so that the solubilized nitrogen was the same. In addition, the amount of salt added to the menu was adjusted so that the salt concentration in the menu would be the same regardless of which bean plate soy was used.
The finished umami of mapo tofu was evaluated in the same manner as in Example 19 by 20 professional panelists. The results are shown in Table 8.
その結果試作豆板醤使用品はうま味において最も好ましいことがわかった。 As a result, it was found that the prototype soy sauce product was most preferable for umami.
（メニュー系での評価 〜麻婆春雨）
実施例１９で作製した試作豆板醤、半年もの豆板醤、及び３年もの豆板醤を用いて、麻婆春雨を試作した。レシピ：材料 牛薄切り肉 ３００ｇ、春雨 １００ｇ、長ねぎ １カップ、にんにく 適量、しょうが 適量、鶏がらスープの素 小さじ１、湯 カップ１、豆板醤 大さじ１と１／２、サラダ油 適量、ゴマ油適量。 作り方：牛肉は食べやすい大きさに切り、醤油・酒・胡椒・ゴマ油少々えで下味をつけた。春雨は熱湯で２，３分固めに茹でて水にさらし、食べやすい長さに切った。ネギは荒みじん、ニンニクとショウガはみじん切りに刻んだ。フライパンにサラダ油を熱し、牛肉を炒める。肉の色が変わったら春雨を加えて混ぜ、鶏がらスープの素を溶かしたお湯を加えた。煮立ったところへ、豆板醤を加えて混ぜ、仕上げにゴマ油をまわしかけた。豆板醤は可溶化窒素が同じになる分量添加した。また、メニューに添加する食塩量を調整して、いずれの豆板醤を用いた場合でもメニュー中の塩分濃度が同一になるように調整した。
出来上がった、麻婆春雨のうま味を２０名の専門パネラーにて実施例１９と同様に評価した。その結果を表９に示す。
(Evaluation in the menu system-Maple vermicelli)
Using the prototype soybean plate soy produced in Example 19, the half-year soybean plate soy, and the three-year soybean plate soy, the mushroom vermicelli was experimentally produced. Recipe: Ingredients: Beef slices 300g, vermicelli 100g, long onion 1 cup, garlic appropriate amount, ginger appropriate amount, chicken tuna soup stock 1 teaspoon, hot water cup 1, bean plate soup 1 1/2 tablespoon, salad oil appropriate amount, sesame oil appropriate amount. How to make: Cut the beef into an easy-to-eat size and season it with a little soy sauce, sake, pepper and sesame oil. The vermicelli was boiled in hot water for a few minutes, exposed to water, and cut to a length that was easy to eat. Green onions were chopped, and garlic and ginger were chopped. Heat salad oil in a frying pan and fry beef. When the color of the meat changed, we added vermicelli and mixed it, and then added hot water in which the chicken broth soup was dissolved. To the boiled place, bean plate soy was added and mixed, and sesame oil was sprinkled on the finish. Soy sauce was added in an amount so that the solubilized nitrogen was the same. In addition, the amount of salt added to the menu was adjusted so that the salt concentration in the menu would be the same regardless of which bean plate soy was used.
The umami of maple vermicelli was evaluated in the same manner as in Example 19 by 20 professional panelists. The results are shown in Table 9.
その結果試作豆板醤使用品はうま味において最も好ましいことがわかった。 As a result, it was found that the prototype soy sauce product was most preferable for umami.
（メニュー系での評価 〜麻婆茄子）
実施例１９で作製した試作豆板醤、半年もの豆板醤、及び３年もの豆板醤を用いて、麻婆茄子を試作した。レシピ：材料 なす ６本、豚ひき肉 １２０ｇ、コーン油 少々、合わせ調味料Ａ（酒 小さじ２、胡椒 少々）、Ｂ（ねぎ １／４本、しょうが ２／３片分、にんにく１片（各微塵切り））、豆板醤 小さじ１と１／２、合わせ調味料Ｂ（水 カップ２／３、鶏がらスープ 小さじ１、しょうゆ 大さじ１と１／２、酒 大さじ２、砂糖 小さじ２、胡椒 少々）、水溶き片栗粉（片栗粉 大さじ１、水 大さじ１）、ごま油 小さじ２。 作り方：なすは縦８等分に切った。豚ひき肉は合わせ調味料Ａで下味をつけた。中華なべに油を適量熱し、豚ひき肉、Ｂを入れて炒め、パラパラになったらなすを加えて更に炒めた。豆板醤を入れてサッと炒め、Ｃを加えて、煮込んだ。水溶き片栗粉でトロミをつけ、ごま油を加え、仕上げた。豆板醤は可溶化窒素が同じになる分量添加した。また、メニューに添加する食塩量を調整して、いずれの豆板醤を用いた場合でもメニュー中の塩分濃度が同一になるように調整した。
出来上がった、麻婆茄子のうま味を２０名の専門パネラーにて実施例１９と同様に評価した。その結果を表１０に示す。
(Evaluation in the menu system-Mariko)
Using the prototype soybean plate soy produced in Example 19, the half-year soybean plate soy, and the three-year soybean plate soy, the eggplant was prototyped. Recipe: Ingredients: 6 eggplants, ground pork 120g, corn oil a little, seasoning A (2 teaspoons of liquor, pepper a little), B (1/4 onion, 2/3 ginger, 1 piece of garlic )), 1 to 1/2 teaspoon bean soy sauce, seasoning B (water cup 2/3, 1 teaspoon chicken soup, 1 and 1/2 tablespoon soy sauce, 2 tablespoons sake, 2 teaspoons sugar, a little pepper) Starch flour (1 tablespoon starch, 1 tablespoon water), 2 teaspoons sesame oil. How to make: Eggplant was cut into 8 equal parts. Minced pork was seasoned with combined seasoning A. Heat a suitable amount of oil in a wok, add minced pork and B, stir-fry, add eggplant when it becomes flipping, and fry further. Stir-fried bean plate soy, add C and simmer. Tromomi with water-soluble potato starch, sesame oil was added and finished. Soy sauce was added in an amount so that the solubilized nitrogen was the same. In addition, the amount of salt added to the menu was adjusted so that the salt concentration in the menu would be the same regardless of which bean plate soy was used.
The finished umami taste was evaluated in the same manner as in Example 19 by 20 professional panelists. The results are shown in Table 10.
その結果試作豆板醤使用品はうま味において最も好ましいことがわかった。 As a result, it was found that the prototype soy sauce product was most preferable for umami.
（メニュー系での評価 〜魚香茄子）
実施例１９で作製した試作豆板醤、半年もの豆板醤、及び３年もの豆板醤を用いて、魚香茄子を試作した。レシピ：材料 茄子２５０ｇ、豚モモ肉 ５０ｇ、卵白 １個分、泡椒 ６ｇ、豆板醤 ３０ｇ、にんにく ４ｇ、ネギ ４．５ｇ、サラダ油 ２０ｇ、酒 ８ｇ、砂糖 ６ｇ、水 ６ｇ、水溶き片栗粉 ６ｇ、醤油 １４ｇ、ゴマ油 ４．８ｇ。作り方：豚モモ肉は棒状にカットした後、塩を加えてよく混ぜた後、卵白で和えて低温の油で揚げた。茄子は棒状にカットし、低温の油でじっくり揚げた。ネギは千切りにした。弱火でネギ、にんにく、泡菜を炒める。香りがでたら豆板醤を炒めた。下ごしらえをした肉、茄子、水、酒、醤油、砂糖を加え、さらに炒めた。水溶き片栗粉を加え、とろみ付けをし、最後にゴマ油をまわしいれた。豆板醤は可溶化窒素が同じになる分量添加した。また、メニューに添加する食塩量を調整して、いずれの豆板醤を用いた場合でもメニュー中の塩分濃度が同一になるように調整した。
出来上がった、魚香茄子のうま味を２０名の専門パネラーにて実施例１９と同様に評価した。その結果を表１１に示す。
(Evaluation in the menu system-Yuko Kouka)
Fish coconut was made using the prototype soy sauce prepared in Example 19, half-year soy sauce, and three-year soy sauce. Recipe: Ingredients 250 g eggplant, 50 g pork leg, 1 egg white, 6 g foam, 30 g bean sauce, 4 g green onion, 4.5 g green onion, 20 g salad oil, 8 g liquor, 6 g sugar, 6 g water, 6 g water soluble starch, 14 g soy sauce , 4.8 g of sesame oil. How to make: Cut pork meat into sticks, add salt and mix well, then mix with egg white and fry in low temperature oil. The eggplant was cut into rods and fried with low temperature oil. Sliced leek. Fry green onions, garlic, and Awana on low heat. When the scent came out, fried bean plate sauce. Prepared prepared meat, eggplant, water, sake, soy sauce and sugar, and fried. I added water-soluble starch, thickened, and finally sprinkled with sesame oil. Soy sauce was added in an amount so that the solubilized nitrogen was the same. In addition, the amount of salt added to the menu was adjusted so that the salt concentration in the menu would be the same regardless of which bean plate soy was used.
The finished savory fish flavor was evaluated in the same manner as in Example 19 by 20 professional panelists. The results are shown in Table 11.
その結果試作豆板醤使用品はうま味において最も好ましいことがわかった。 As a result, it was found that the prototype soy sauce product was most preferable for umami.
（麻棒豆腐の殺菌条件の検討：一定殺菌条件下での芽胞菌数の比較）
実施例２０で調理した麻婆豆腐を各々レトルトパウチに１５０ｇ加えて、１２０℃で４分間加熱殺菌した。その結果、試作豆板醤を使用した麻婆豆腐からは芽胞菌は検出されなかったが、半年もの豆板醤及び３年もの豆板醤を使用した麻婆豆腐からでは完全殺菌できなかった。
(Examination of sterilization conditions of hemp stick tofu: Comparison of the number of spore bacteria under certain sterilization conditions)
150 g of mapo tofu cooked in Example 20 was added to each retort pouch and sterilized by heating at 120 ° C. for 4 minutes. As a result, no spore bacteria were detected from the mapo tofu using the prototype soy sauce, but it could not be completely sterilized from the mapo tofu using the half-year soy sauce and the three-year soy sauce.
（麻棒豆腐の殺菌条件の検討：芽胞菌が検出されない殺菌条件）
実施例２０で調理した麻婆豆腐を各々レトルトパウチに１５０ｇ加えて１２０℃で加熱殺菌した。完全殺菌できた条件は各々本発明の豆板醤は１２０℃で４分、半年ものは１２０℃で１２分以上、３年もので１２０℃で１２分以上であった。芽胞菌が検出されない条件で殺菌した麻婆豆腐のうま味を２０名の専門パネラーにて評価した結果、全員に試作豆板醤が好まれた。
(Examination of sterilization conditions of hemp stick tofu: sterilization conditions in which spore bacteria are not detected)
150 g of mapo tofu cooked in Example 20 was added to each retort pouch and sterilized by heating at 120 ° C. The conditions for complete sterilization were 120 ° C. for 4 minutes for the bean plate soy of the present invention, 12 minutes or more for 120 minutes at 120 ° C. As a result of evaluating the umami of mapo tofu sterilized under the condition that no spore bacteria were detected by 20 expert panelists, everyone liked the prototype soy sauce.
（殺菌された豆板醤を使用した麻棒豆腐の比較）
半年もの豆板醤及び３年もの豆板醤を各々レトルトパウチに１５０ｇ加えて１２０℃で殺菌した。完全殺菌できた条件は半年もの、３年もの共、１２０℃で１２分以上であった。次に実施例１９で試作した実質的に殺菌状態にある豆板醤及び殺菌した半年もの豆板醤、及び殺菌した３年もの豆板醤を用いて実施例２０と同様に麻婆豆腐を調理した。麻婆豆腐のうま味を２０名の専門パネラーにて評価した結果、全員に試作豆板醤が好まれた。
(Comparison of hemp stick tofu using sterilized bean plate soy)
150 g of half-year soy sauce and three-year soy sauce were added to the retort pouch and sterilized at 120 ° C. The conditions for complete sterilization were 12 minutes or more at 120 ° C. for 6 months or 3 years. Next, the mapo tofu was cooked in the same manner as in Example 20 using the substantially sterilized bean plate soy produced in Example 19, the sterilized half-year bean plate soy, and the sterilized three-year bean plate soy. As a result of evaluating the umami of mapo tofu by 20 expert panelists, everyone liked the prototype soy sauce.
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