JP2006228185A - Microscope observation reproducing method, microscope observation reproducing apparatus, microscope observation reproducing program and record medium thereof - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microscope observation reproducing method, and its apparatus, program and record medium, displaying an animation reproducing the microscope observation and automatically jetting and displaying description on a specified region on the animation arbitrarily. <P>SOLUTION: Concerning a plurality of grouping areas whose peripehral parts overlap each other in a photographing object area of a microscope image, the data of a plurality of screening and zooming static images photographed at the highest magnification and the data of a plurality of focusing static images photographed with variable focal points in the respective grouping areas are created. From the data of screening and zooming composite static image obtained by conducting predetermined processing for the data of the static image, and the already edited focusing static image data, an animaiton for screening, an animation for zooming, and an animation for focusing are created and displayed on a computer screen. When a mouse cursor is located at a specified region on the animation, jetting and description of the concerned specified region are displayed. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to reproduction on a computer of microscopic observation performed in histopathological examination, cytodiagnosis, and the like, and to an ejection explanation display at that time.

  Conventionally, when presenting a microscopic image of a histopathological specimen or cytodiagnosis specimen at an academic conference or workshop, the still image has been displayed using a slide or a photograph. However, since the specimen information obtained from a microscope is detailed and diverse, only a limited amount of specimen information can be conveyed in a still image. In addition, still images taken in cytodiagnosis largely depend on the judgment of laboratory technicians and doctors, and of course, there is a limit to objective judgment.

  Therefore, the inventor of the present invention has already proposed a microscopic observation performed by histopathological examination, cytodiagnosis, or the like that is accurately reproduced with a moving image on a computer (see Non-Patent Document 1).

  The moving image proposed here is intended to reproduce various operations on a computer when observing with a microscope. That is, in microscopic observation, for example, screening of cytological specimens (movement observation), zooming (magnification observation) and focusing (stereoscopic observation) of sensitive cells, and confirmation of strong enlargement (partial) from weak enlargement (entire) of tissue specimen Observe while performing various operations. In the above proposal, a moving image (called Animated Microscopic Image) that reproduces the movement of the microscope image seen during screening, zooming, and focusing in actual microscope observation is displayed on the computer screen, and the user performs screening, zooming, and focusing. When an instruction operation is input to a computer, a moving image having the same movement as an actual microscopic image is reproduced according to the input. The operation input is performed by operating a screening button, a zooming button, a focusing button displayed on the computer screen using a mouse or the like, or dragging an image.

For example, when a screening operation is input, the microscope image displayed on the computer screen moves in the screening direction. That is, the microscopic images that actually appear in the screening direction are projected smoothly one after another. If a zooming operation is further input at this time, the microscope image on the computer screen is enlarged or reduced. In other words, a microscopic image that actually appears enlarged or reduced is displayed instantly. As a result, the tissues and cells caught by the eyes can be checked by magnifying while observing a weakly magnified image over a wide range, and screening and zooming are faithfully reproduced. Of course, the same applies to focusing.
Kiyadamu Sugaya, “Microscope Imaging Technology that reproduces microscopic observation”, [online], February 3, 2003, Cytologists Association, http://www.intercyto.com/research/MIT1/index.htm

  By the way, in the said nonpatent literature 1, it is only the indication about the display of reproduction animation, and its operation, and there is no concrete indication about the concrete creation method of the animation. Further, if it is possible to display the squirting explanation regarding the specific part of the moving image as described above, it is considered that further effective presentation and learning can be realized. For example, a detailed virtual microscope learning can be performed by automatically displaying a description of the cells or tissues requiring attention such as atypical cells and the like which are considered important for learning instruction.

  Therefore, in view of the circumstances as described above, the present invention provides a microscope observation reproduction method, a microscope observation reproduction device, a microscope observation reproduction program, and a recording medium thereof that can reproduce various operations when observing with a microscope on a computer. It is an issue to provide.

  Another object of the present invention is to provide a microscope observation reproduction method, a microscope observation reproduction device, a microscope observation reproduction program, and a recording medium thereof that can display the ejection explanation regarding the specific part of the moving image.

In order to solve the above problems, the present invention firstly,
A plurality of still images for screening and zooming that have been photographed at the maximum magnification for magnifying and observing a plurality of segmented regions obtained by segmenting a target region of the microscopic image so that a part of the peripheral portion overlaps each other. Accept data input,
The processing unit receives input of data of a plurality of still images for focusing that are photographed by changing the focus in each of the divided areas,
The processing unit stores a plurality of input still image data for screening and zooming and a plurality of input still image data for focusing in a storage means,
The processing unit retrieves the data of the plurality of still images for screening and zooming from the storage means, and after joining these data, cuts out a necessary range and obtains data of the combined still image for screening and zooming. Creating and storing the data of the still image for screening and zooming in the storage means,
The processing unit takes out the data of the plurality of focusing still images from the storage unit, edits the data of each focusing still image with the same accuracy, creates edited focusing still image data, Store the edited still image data for focusing in the storage means,
The processing unit takes out the data of the screening and zooming composite still image from the storage unit, creates a moving image for screening and a moving image for zooming, and stores the moving image data in the storage unit,
The processing unit takes out edited focusing still image data from the storage unit to create a moving image for focusing, and stores the moving image data in the storage unit,
There is provided a microscope observation reproduction method, wherein the processing unit displays a moving image reproducing a movement of a microscope image seen during screening, zooming, and focusing in actual microscope observation on a computer screen.

  Second, in the first invention, a moving image that reproduces microscopic observation is displayed on a computer screen, and when a mouse cursor is positioned at a specific portion on the moving image, an ejection description relating to the specific portion is displayed. Provided is a microscopic observation reproduction method characterized.

  Third, a microscope observation reproduction method characterized in that a moving image for reproducing a microscope observation is displayed on a computer screen, and an ejection explanation relating to the specific portion is displayed when the mouse cursor is positioned at the specific portion on the moving image. I will provide a.

In addition, the present invention fourthly,
Inputs data for multiple still images for screening and zooming taken at the highest magnification for magnification observation of a plurality of divided areas obtained by dividing the imaging target area of the microscopic image so that a part of the periphery overlaps each other. means,
Means for accepting input of data of a plurality of still images for focusing, which are photographed by changing the focus in each of the divided areas;
Means for storing a plurality of input still image data for screening and zooming and a plurality of input still image data for focusing in a storage means;
The plurality of still images for screening / zooming are extracted from the storage means, and after combining these data, a necessary range is cut out to create synthetic still image data for screening / zooming. Means for storing data of a composite still image for zooming in the storage means;
The data of the plurality of focusing still images is extracted from the storage means, the data of each focusing still image is edited with the same accuracy, and the edited focusing still image data is created, and the edited focusing still image data is created. Means for storing image data in the storage means;
Means for taking out the still image data for screening and zooming from the storage means, creating a moving image for screening and a moving image for zooming, and storing the moving image data in the storage means;
The edited still image data for focusing is taken out from the storage means to create a moving image for focusing, and the moving image data is stored in the storage means, and it is observed during screening, zooming, and focusing in actual microscope observation. There is provided a microscope observation reproduction apparatus characterized by comprising means for displaying on a computer screen a moving image that reproduces the movement of a microscope image.

  Fifth, in the fourth aspect of the present invention, further, means for storing in advance an ejection description relating to the specific part on the moving image, and when the mouse cursor is positioned on the specific part on the displayed moving image Provided is a microscope observation reproduction device characterized by comprising means for reading out and displaying the ejection description relating to the specific part.

Sixth, means for creating a moving image that reproduces microscopic observation,
Means for displaying the created video,
Means for storing in advance a description of a specific part on the moving image, and reading and displaying the description of the specific part when the mouse cursor is positioned on the specific part on the displayed moving image Provided is a microscope observation reproduction apparatus characterized by comprising means.

In addition, the present invention, seventhly,
Input the data of multiple still images for screening and zooming at the maximum magnification for magnifying the multiple target areas of the microscopic image that are divided so that part of the periphery overlaps each other. Means to accept,
Means for accepting input of data of a plurality of still images for focusing, which are photographed by changing the focus in each of the divided areas;
Means for storing a plurality of input still image data for screening and zooming and a plurality of input still image data for focusing in a storage means;
The plurality of still images for screening / zooming are extracted from the storage means, and after combining these data, a necessary range is cut out to create synthetic still image data for screening / zooming. Means for storing data of a composite still image for zooming in the storage means;
The data of the plurality of focusing still images is extracted from the storage means, the data of each focusing still image is edited with the same accuracy, and the edited focusing still image data is created, and the edited focusing still image data is created. Means for storing image data in the storage means;
Means for taking out the still image data for screening and zooming from the storage means, creating a moving image for screening and a moving image for zooming, and storing the moving image data in the storage means;
The edited still image data for focusing is taken out from the storage means to create a moving image for focusing, and the moving image data is stored in the storage means, and it is observed during screening, zooming, and focusing in actual microscope observation. There is provided a microscope observation reproduction program characterized by causing a computer to function as means for displaying a moving image reproducing a movement of a microscope image displayed on a computer screen.

  Eighth, in the seventh aspect of the invention, further, means for storing in advance a blow-out explanation regarding the specific part on the moving image, and when the mouse cursor is positioned on the specific part on the displayed moving image There is provided a microscope observation reproduction program characterized by comprising means for reading out and displaying the ejection description relating to the specific part and causing a computer to function.

Ninth, means for creating a moving image that reproduces microscopic observation,
Means for displaying the created video,
Means for storing in advance a description of a specific part on the moving image, and reading and displaying the description of the specific part when the mouse cursor is positioned on the specific part on the displayed moving image A microscope observation reproduction program characterized by causing a computer to function as means is provided.

  Furthermore, the present invention provides, in a tenth aspect, a computer-readable recording medium in which the microscope observation reproduction program according to any one of the seventh to ninth aspects is recorded.

  According to the above-described microscope observation method and microscope observation apparatus, it is possible to accurately reproduce on a computer a moving image that reproduces microscope observation (hereinafter referred to as “AMI”). In addition, during the display, for example, it is possible to automatically display the squirting explanation for specific parts such as cells requiring attention such as atypical cells and tissues that are important for learning instruction, and tissues, thereby enabling detailed virtual display. Microscope learning becomes possible.

  According to the above-described microscope observation program and recording medium, a computer program capable of obtaining the same effects as those of the above-described microscope observation method and microscope observation apparatus and a recording medium such as a flexible disk, a CD, and a DVD on which the computer program is recorded are realized. The

  Hereinafter, an embodiment of the present invention will be described.

  First, an embodiment of a method for creating an AMI reproduced as a moving image for microscopic observation according to the present invention will be described. Here, an example is described in which an image of a cytodiagnosis microscope specimen is handled, but the present invention is not limited thereto.

  (1) The AMI image is taken as a digital image using, for example, a digital camera or a high-vision camera dedicated to a microscope. For AMI creation, image editing / compositing (Tiling) software and moving image creation software are used. Commercially available software such as Photoshop Elements 2.0 (registered trademark: manufactured by Adobe) can be used as image editing / synthesizing software, for example. Hotmedia Creator etc. attached to the registered trademark (made by IBM Corporation) can be used. Below, it demonstrates using the example software here.

(2) Digital image shooting (i) Screening / zooming image Decide the range you want to shoot and shoot all the fields of interest at the maximum magnification for magnified observation. Since the images are combined, the images are taken so that, for example, 10% or more of the ranges overlap.

(Ii) Focusing image Since cells overlap each other in the cell clump seen in the cytodiagnosis specimen, the image changes for each focal point. Take all the images in focus.

(3) Image editing (i) Screening / zooming images The captured images are combined (tiled) with the image editing / compositing software Photoshop elements 2.0 (registered trademark: manufactured by Adobe). When “Photomerge” is selected from the file menu and all the images to be combined are selected with the “reference button” of the source file (FIG. 1A), the images are automatically combined (FIG. 1B). Since composition is performed in units of pixels, no image shift is observed. The synthesized image is cut out (FIG. 1 (c)), and the file name after editing is saved (FIG. 1 (d)).

(Ii) Focusing image The obtained image is edited with the same accuracy. Here, editing refers to stacking a plurality of images picked up with different focal points in a certain segmented region in the focusing direction (direction perpendicular to the plane on which the image exists). The image for focusing is not synthesized. For details on how to edit and maintain the accuracy of digital images, for example, Kiyadamu Sugaya “Operation of Internet Survey”, Medical Technology, 2001, 1: p.75-81, Kiyotada Sugaya “The 43rd Cytology Seminar (Sendai)” , Handout, p. 67-76, 2001.

(4) Movie Creation Processing for creating a movie (AMI) with the movie creation software HotMedia creator will be described.

(I) Screening / zooming images
The scroll image on the HotMedia creator screen is checked, and the image synthesized with the “add button” is read (FIG. 2A). An image is set (maximum enlargement ratio: 3 initial movement: 0 initial display: 1) with the “property button” (FIG. 2B) and saved as a Web page from the file menu (FIG. 2C). Here, mvr (video) and html are created, so open the html source file and change the size of the image size “(WIDTH, HEIGHT)” surrounded by “APPELET” to the size you want to display on your computer. (FIG. 2D). AMI is executed by starting an html file.

(Ii) Focusing image
Check the animation on the HotMedia creator startup screen and read the image taken for focusing with the “Add button” (FIG. 3A). Set the image (automatic animation playback: loop default transition time [display interval time]: 3000) with the “property button” (FIG. 3B), and name two file names mvr and html as Web pages. Add and save. When html file is activated, AMI is activated.

  Next, a description will be given of a method for creating an AMI that can be displayed with explanation.

  The mvr file created by HotMedia creator is specified (double-clicked) and opened (FIG. 4 (a)), and the opened image is double-clicked and popped up to create a jet with commentary. Next, when the mouse (drag) is dragged to surround the cell (part) to be explained, a “hot link trigger setting screen” is opened (FIG. 4B). Therefore, the “mouse over action edit button” is clicked, and a description is entered in the “tool hint field” (FIG. 4C). By repeating the operations of FIG. 4B and FIG. 4C, a plurality of ejection explanations can be set (FIG. 4D). The html file also has an explanation function called “Alt tag”, but it disappears in a few seconds. In that respect, the explanation eruption by the method described here does not disappear while pointing, so there is an advantage that it can be studied thoroughly when used as a learning material.

  Next, an embodiment of the present invention capable of displaying the ejection explanation will be described in detail with reference to FIG. FIG. 5 is a system configuration diagram of the microscope observation apparatus of the present invention.

  First, the processing unit 3 receives a command from a program stored in the main storage unit 4 and divides the imaging target region in the microscopic image into a plurality of segmented regions that are segmented so that a part of the peripheral portions overlap each other. The digital data of a plurality of still images for screening and zooming taken at the maximum magnification for magnifying observation is taken in from the input unit 2. Further, the processing unit 3 takes in digital data of a plurality of still images for focusing, which are photographed by changing the focus in each of the divided areas, from the input unit 2. Then, the processing unit 3 causes the still image database 5 to store the plurality of input still image data for screening and zooming and the plurality of input still image data for focusing.

  Subsequently, the processing unit 3 takes out the data of the plurality of still images for screening / zooming from the still image database 5, joins these data, cuts out a required range, and combines the still images for screening / zooming. Create image data. Then, the processing unit 3 stores the data of the screening / zooming composite still image in the still image database 5.

  Further, the processing unit 3 takes out the data of the plurality of focusing still images from the still image database 5, edits the data of each focusing still image with the same accuracy, and creates edited still image data for focusing To do. Then, the processing unit 3 causes the still image database 5 to store the created edited still image data for focusing.

  Next, the processing unit 3 takes out the data of the combined still image for screening and zooming from the still image database 5, creates a moving image for screening and a moving image for zooming, and stores the moving image data in the moving image database 6.

  In addition, the processing unit 3 extracts edited focusing still image data from the still image database 5 to create a moving image for focusing, and stores the moving image data in the moving image database 6.

  The AMI is created as described above.

  When displaying the AMI, first, the processing unit 3 receives an instruction of a program stored in the main storage unit 4, reads out the AMI stored in the moving image database 6, and displays it on the display unit 1.

  Subsequently, the processing unit 3 displays a mouse cursor on the displayed AMI. The mouse cursor moves on the AMI in conjunction with the mouse operation by the user.

  When the user inputs operations for instructing screening, zooming, and focusing to the computer, a moving image that has the same movement as the actual microscopic image is reproduced according to the input. The operation input is performed by operating a screening button, a zooming button, a focusing button displayed on the computer screen using a mouse or the like, or dragging an image.

  When an operation for screening is input, the microscope image displayed on the computer screen moves in the screening direction, and the microscope images that actually appear in the screening direction are displayed smoothly one after another. If a zooming operation is further input at this time, the microscope image on the computer screen is enlarged or reduced. As a result, the tissues and cells caught by the eyes can be checked by magnifying while observing a weakly magnified image over a wide range, and screening and zooming are faithfully reproduced. Of course, the same applies to focusing.

  In the case where the ejection explanation regarding the specific site on the AMI is also performed, in this case, when the mouse cursor is positioned at the specific site on the AMI, the processing unit 3 displays the ejection explanation regarding the specific site. Regarding the ejection explanation, one or more specific parts on the AMI are set in advance, and the ejection explanation data is created for each specific part and stored in the explanation database 7. The processing unit 3 tracks the movement of the mouse cursor on the AMI, and when the mouse cursor is positioned at the set position of the specific part, the ejection explanation data of the specific part is read from the explanation database 7 and displayed instantaneously. Let

  As a result, the user can see a specific explanation of the part only by moving the mouse cursor to the part of interest while looking at the AMI, and can learn important parts while simulating a microscope operation. .

  FIGS. 6A to 6D each show a specific example in which a description of ejection is displayed.

  First, as shown in FIG. 6A, a mouse cursor is displayed on the AMI of the cytodiagnosis specimen displayed on the computer screen, and this mouse cursor moves on the AMI in conjunction with the mouse operation by the user. When a part that seems to be important for learning is reached while moving, a frame of a region including the part is displayed as shown in FIG. Next, when the inside of the frame is clicked, the AMI within the frame is enlarged and displayed as shown in FIG. Further, when the mouse cursor reaches one of the parts considered to be important for learning in the enlarged AMI, the ejection explanation regarding the part is displayed as shown in FIG. At this time, the user only has to move the mouse cursor on the AMI by operating the mouse, and the ejection explanation is automatically displayed when the mouse point is located at a specific part.

  Thus, in this example, the following operation is possible as a whole.

  First, by inputting a screening operation, the microscope image displayed on the computer screen moves in the screening direction. That is, the microscopic images that actually appear in the screening direction are projected smoothly one after another.

  If a zooming operation is further input at this time, the microscope image on the computer screen is enlarged or reduced. In other words, a microscopic image that actually appears enlarged or reduced is displayed instantly. As a result, the tissues and cells caught by the eyes can be checked by magnifying while observing a weakly magnified image over a wide range, and screening and zooming are faithfully reproduced.

  Further, when an operation for focusing is performed, for example, when a certain cell cluster is observed three-dimensionally, the focusing is similarly faithfully reproduced.

  Furthermore, when the user moves to the part that is considered important for learning while moving the mouse cursor on the AMI, a frame of the area including the part is displayed, and the ejection explanation regarding the part is displayed.

  Therefore, since the microscopic observation of the target specimen can be accurately reproduced on a computer as described above, it is extremely useful not only for presentations at academic conferences but also for microscope learning.

  Of course, the present invention is not limited to the above-described embodiments, and various aspects are possible for details. For example, the present invention is considered to be applicable not only to microscopic observation, but also to endoscopic observation and naked-eye observation. What is necessary is just to enable it to display an explanation blowout arbitrarily, reproducing a moving image.

(A)-(d) is explanatory drawing of edit of the still image for screening zooming. (A)-(d) is explanatory drawing of the moving image creation of the image for screening zooming. (A), (b) is explanatory drawing of the moving image creation of the image for focusing. (A)-(d) is explanatory drawing of the production method of AMI which can be ejected and displayed with description. The system block diagram which showed one Embodiment of this invention. (A)-(d) is a hard copy figure of the computer screen in the case of each displaying an ejection description.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Display part 2 Input part 3 Processing part 4 Main memory part 5 Still image database 6 Movie database 7 Explanation database 8 Bus

Claims (10)

A plurality of still images for screening and zooming that have been photographed at the maximum magnification for magnifying and observing a plurality of segmented regions obtained by segmenting a target region of the microscopic image so that a part of the peripheral portion overlaps each other. Accept data input,
The processing unit receives input of data of a plurality of still images for focusing that are photographed by changing the focus in each of the divided areas,
The processing unit stores a plurality of input still image data for screening and zooming and a plurality of input still image data for focusing in a storage means,
The processing unit retrieves the data of the plurality of still images for screening and zooming from the storage means, and after joining these data, cuts out a necessary range and obtains data of the combined still image for screening and zooming. Creating and storing the data of the still image for screening and zooming in the storage means,
The processing unit takes out the data of the plurality of focusing still images from the storage unit, edits the data of each focusing still image with the same accuracy, creates edited focusing still image data, Store the edited still image data for focusing in the storage means,
The processing unit takes out the data of the screening and zooming composite still image from the storage unit, creates a moving image for screening and a moving image for zooming, and stores the moving image data in the storage unit,
The processing unit takes out edited focusing still image data from the storage unit to create a moving image for focusing, and stores the moving image data in the storage unit,
A microscope observation reproduction method, wherein the processing unit displays a moving image reproducing a movement of a microscope image seen during screening, zooming, and focusing in actual microscope observation on a computer screen.   2. The microscope observation according to claim 1, wherein a moving image reproducing the microscopic observation is displayed on a computer screen, and when the mouse cursor is positioned at a specific portion on the moving image, an ejection explanation regarding the specific portion is displayed. Reproduction method.   A method for reproducing a microscope observation, characterized in that a moving image for reproducing a microscopic observation is displayed on a computer screen, and when a mouse cursor is positioned at a specific portion on the moving image, an ejection explanation regarding the specific portion is displayed. Inputs data for multiple still images for screening and zooming taken at the highest magnification for magnification observation of a plurality of divided areas obtained by dividing the imaging target area of the microscopic image so that a part of the periphery overlaps each other. means,
Means for accepting input of data of a plurality of still images for focusing, which are photographed by changing the focus in each of the divided areas;
Means for storing a plurality of input still image data for screening and zooming and a plurality of input still image data for focusing in a storage means;
The plurality of still images for screening / zooming are extracted from the storage means, and after combining these data, a necessary range is cut out to create synthetic still image data for screening / zooming. Means for storing data of a composite still image for zooming in the storage means;
The data of the plurality of focusing still images is extracted from the storage means, the data of each focusing still image is edited with the same accuracy, and the edited focusing still image data is created, and the edited focusing still image data is created. Means for storing image data in the storage means;
Means for taking out the still image data for screening and zooming from the storage means, creating a moving image for screening and a moving image for zooming, and storing the moving image data in the storage means;
The edited still image data for focusing is taken out from the storage means to create a moving image for focusing, and the moving image data is stored in the storage means, and it is observed during screening, zooming, and focusing in actual microscope observation. A microscope observation reproduction apparatus, characterized by comprising means for displaying on a computer screen a moving image that reproduces the movement of a microscope image. Further, means for storing in advance a description of the specific part on the moving image, and reading the description of the specific part regarding the specific part when the mouse cursor is positioned at the specific part on the displayed moving image The microscope observation reproduction apparatus according to claim 4, further comprising a display unit. A means to create a movie that reproduces microscopic observation,
Means for displaying the created video,
Means for storing in advance a description of a specific part on the moving image, and reading and displaying the description of the specific part when the mouse cursor is positioned on the specific part on the displayed moving image A microscope observation reproduction apparatus characterized by comprising means. Input the data of multiple still images for screening and zooming at the maximum magnification for magnifying the multiple target areas of the microscopic image that are divided so that part of the periphery overlaps each other. Means to accept,
Means for accepting input of data of a plurality of still images for focusing, which are photographed by changing the focus in each of the divided areas;
Means for storing a plurality of input still image data for screening and zooming and a plurality of input still image data for focusing in a storage means;
The plurality of still images for screening / zooming are extracted from the storage means, and after combining these data, a necessary range is cut out to create synthetic still image data for screening / zooming. Means for storing data of a composite still image for zooming in the storage means;
The data of the plurality of focusing still images is extracted from the storage means, the data of each focusing still image is edited with the same accuracy, and the edited focusing still image data is created, and the edited focusing still image data is created. Means for storing image data in the storage means;
Means for taking out the still image data for screening and zooming from the storage means, creating a moving image for screening and a moving image for zooming, and storing the moving image data in the storage means;
The edited still image data for focusing is taken out from the storage means to create a moving image for focusing, and the moving image data is stored in the storage means, and it is observed during screening, zooming, and focusing in actual microscope observation. A microscope observation reproduction program characterized by causing a computer to function as a means for displaying on a computer screen a moving image that reproduces the movement of a microscope image. Further, means for storing in advance a description of the specific part on the moving image, and reading the description of the specific part regarding the specific part when the mouse cursor is positioned at the specific part on the displayed moving image 6. The microscope observation reproduction program according to claim 5, further comprising means for displaying and causing a computer to function. A means to create a movie that reproduces microscopic observation,
Means for displaying the created video,
Means for storing in advance a description of a specific part on the moving image, and reading and displaying the description of the specific part when the mouse cursor is positioned on the specific part on the displayed moving image A microscope observation reproduction program characterized by causing a computer to function as means.   A computer-readable recording medium on which the microscope observation reproduction program according to claim 7 is recorded.