JP2006226752A - Plate for bio-sample discrimination device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a small-sized inexpensive plate for a bio-sample discrimination device which dispenses with complicated preparation work and can obtain an accurate result in a short time when a bio-sample is discriminated by detecting transport reaction obtained when the bio-sample is moved within the buffering agent charged in a flow channel. <P>SOLUTION: The plate for the bio-sample discrimination device is constituted of a positive electrode layer, which is constituted so that the positive electrode contact part 24 arranged on the outer peripheral part of the plate in an arcuate state is connected to the positive electrode part of the flow channel, and a negative electrode layer, which is constituted so that the negative electrode contact part 25 arranged around the hole of the center part of the plate in an arcuate state is connected to the negative electrode part of the flow channel, on the surface opposite to a flow channel forming surface. Accordingly, by respectively pressing two electrodes to the positive electrode contact part 24 and the negative electrode contact part 25, voltage can be applied to all of the flow channels at the same time. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a plate for a biological sample discriminating apparatus that discriminates a biological sample by detecting a transport reaction obtained by moving a biological sample such as DNA, protein or the like in a buffer.

  When a general biological sample is considered, DNA and protein exist largely. In recent years, with the rapid development of molecular biology, the involvement of genes in various diseases has been understood fairly accurately, and attention has been focused on medical treatments targeting genes.

  With regard to DNA, SNPs (abbreviation of single nucleotide polymorphism, which is generally translated as “single nucleotide polymorphism” and is a general term for the difference of one code (one base) in a gene) are currently attracting attention. The reason for this is that the classification of SNPs can predict the prevalence of many diseases and the effects and susceptibility of each individual to drugs. Furthermore, there are absolutely no humans on the planet who have the same SNPs, even if they are parents and children. This is because it is considered that the individual can be completely identified from not doing so.

  Currently, as a method for examining SNPs, sequencing (determination of base sequence) in which the base sequence of DNA is directly read from the end is most commonly used. There are several reports on the sequencing method, but the most common method is dideoxy sequencing (Sanger method). Sequencing is based on a technique that separates and identifies the difference in length of one base length by denaturing polyacrylamide gel electrophoresis with high resolution or capillary electrophoresis in any method including the Sanger method. This is true.

  Another method is affinity ligand capillary electrophoresis.

  Affinity ligand capillary electrophoresis uses intermolecular affinity, particularly specific affinity in the ecosystem (enzyme and substrate, antigen and antibody affinity, etc.) to give specificity to separation. Specifically, When an affinity ligand that specifically recognizes the base sequence is added to the electrophoresis solution in the capillary tube and the sample is electrophoresed, only the molecular species that interact in the sample mixture will change in the moving speed. The analysis is performed with attention (for example, see Patent Document 1).

  On the other hand, proteins exist in cells, tissues, and biological fluids, regulate biological activities, supply energy to cells, synthesize important substances, maintain biological structures, and communicate between cells and intracellular information. Involved in transmission. Currently, it has become clear that proteins have multiple functions depending on the various environments, the presence of other interacting proteins, and the degree and type of modification that the protein has undergone.

  Proteins are made by connecting 20 types of amino acids in order according to gene instructions (sequence information), and the types are said to be tens of millions. If you know the sequence of the gene, what amino acid is what? You can get information on whether they are connected in order. A complete set of proteins made from the genes (genomes) of organisms is called a proteome, and now the analysis of the proteome has been actively promoted after the nucleotide sequence of the human genome has been deciphered.

Such functional analysis of proteins requires not only identification and characterization, but also biochemical assays, protein-protein interaction studies, protein networks, and intracellular and extracellular signaling elucidation. Research on this protein function uses a variety of techniques, including enzyme assays, yeast two-hybrid assays, chromatographic purification, information tools and databases, etc. Protein discrimination by electrophoresis is particularly important. It is a technique. Then, as in electrophoresis, the transport reaction obtained when moving liquids such as samples, analytes, buffers, and reagents in capillary tubes is detected, and analysis, discrimination, determination, etc. of the samples are performed. There are various reports regarding the transport and orientation of the liquid when performed (for example, Patent Documents 2 to 6).
JP 7-311198 A JP 2002-340859 A Special table 2000-513813 JP-T-2001-523341 Special Table 2000-51428 JP 2003-28883 A

  The present invention provides an accurate detection result in a short time when detecting a transport reaction when a biological sample is moved in a buffer filled in a flow path, is easy to handle, is small, lightweight, and inexpensive. It aims at providing the plate for biological sample discrimination devices.

  As described above, in the analysis of biological samples, a method using a capillary electrophoresis apparatus is widely used. In many cases, a capillary that is a part that actually performs a transport reaction uses a glass tube having an outer diameter of about 300 microns and an inner diameter of about 100 microns, and the surface is coated with polyimide or the like to make it difficult to break. However, in order to detect the internal sample, a part of the coating is peeled off by baking as a detection window or dissolving with chemicals. At this time, the part where the coating is peeled off is easily broken, and it is necessary to be careful in handling. It is still dangerous if it breaks.

  In addition, sample injection is generally performed by pressurization or suction, but it is necessary to inject a certain amount, but the injection amount depends on changes in the viscosity and temperature of the buffer in the capillary, although it is adjusted over time. However, there is a problem that it is different for each experiment. The amount of sample has a great influence on the measurement result and is a very important item.

  In addition, in the case of an apparatus using such a capillary, it is difficult to perform electrophoresis in a short flow path because of the configuration, and there is a problem that the measurement takes an excessive migration distance and time. (See Patent Document 1).

  Moreover, in the gene diagnosis apparatus and diagnosis method of Patent Document 2 described above, it is necessary to fill a capillary tube filled with a buffer containing a linear polymer and a DNA binding control agent with a DNA conjugate for separation and a DNA sample. is there. As described above, it is troublesome to fill each capillary tube with the DNA conjugate for separation and the DNA sample, and when the DNA conjugate for separation and the DNA sample are filled, the amount of them is reduced. If they differ between capillary tubes, the measurement results may be affected.

  Furthermore, in order to separate the sample, for example, in the methods described in Patent Documents 3 and 4, a plurality of the cavities channels are crossed and at least three electrodes are provided, and the at least three electrodes are provided. The sample is applied to two of the electrodes and the sample is moved through the intersecting portion. However, in this method, since the flow paths intersect, there is a possibility that the sample does not migrate well when electrophoresis is performed. There is a problem that an accurate measurement result cannot be obtained. Further, for example, in the methods described in Patent Documents 5 and 6, the sample is moved by changing the centripetal force generated by the rotation by embedding a fine channel in the platform and changing the rotation speed of the platform. In this method, in addition to the problem that the sample can be moved only in the centripetal direction, there is also a problem that the shape of the microchannel is considerably complicated.

  An object of the present invention is to provide a plate for a biological sample discriminating apparatus that does not require complicated preparation work and is accurate when detecting a transport reaction when a biological sample is moved in a buffer material filled in a flow path. And

  In order to solve the above-mentioned conventional problem, for a biological sample discriminating apparatus provided with a plurality of flow paths for detecting a transport reaction obtained by moving a biological sample by electrophoresis in a buffer and discriminating the biological sample In the plate, a pair of electrodes is provided in each of the plurality of channels, and one of the pair of electrodes is electrically connected to an electrode provided in a central portion of the biological sample discriminating apparatus plate. And the other electrode of the pair of electrodes is electrically connected to an electrode provided on an outer peripheral portion of the biological sample discriminating device plate, and an electrode provided at a central portion of the biological sample discriminating device plate and the electrode The biological sample is electrophoresed in a buffer based on a potential difference with an electrode provided on the outer periphery of the biological sample discriminating apparatus plate.

  Furthermore, the electrode is made of a conductive film or a metal film.

  Furthermore, the conductive film is affixed to the surface opposite to the flow path forming surface of the biological sample discrimination device plate.

  Further, the metal film is sandwiched between a plate in which no flow path is formed and a surface opposite to the flow path forming surface of the plate in which the flow path is formed.

  Furthermore, the metal film is any one of copper, aluminum, and gold.

  According to the biological sample discriminating device plate of the present invention, by connecting all the positive electrode application units and all the negative electrode application units, it is possible to apply a voltage to all the flow paths simultaneously with only two electrodes. it can. In addition, even when applying a voltage while centrifuging the biological sample discriminating apparatus plate, the electrode configuration can be simplified because the electrode can be fixed at a certain place.

(Embodiment 1)
Hereinafter, the biological sample discriminating apparatus plate according to the first embodiment will be described with reference to FIGS.

  The present invention realizes that biological samples can be easily and inexpensively and accurately distinguished in a short time by moving biological samples in a buffer and performing biological, enzymatic, immunological and chemical reactions. To do.

  In the first embodiment, for the sake of concrete explanation, it is assumed that the biological sample is a DNA sample and the buffering agent contains a DNA conjugate for separation and a DNA binding control agent. However, by adding the quantified DNA sample to the separation DNA conjugate packed in the flow channel and performing electrophoresis, the fluorescence or absorbance in the flow channel is detected, and the DNA sample The presence or absence of SNPs (single nucleotide polymorphisms) shall be determined.

First, the configuration of the biological sample discriminating apparatus plate according to the first embodiment will be described. FIG. 1 is a diagram showing a flow path forming surface of the biological sample discriminating apparatus plate according to the first embodiment.
FIG. 2 is a diagram showing the detailed shape of the pattern 2 formed on the plate 1 shown in FIG.

  Eight patterns 2 shown in FIG. 2 are formed in the radiation method on the plate 1 in the first embodiment, and DNA discrimination of eight specimens is possible at the same time.

  As shown in FIG. 1, the outer shape of the plate 1 in the first embodiment is a square of 8 cm square, three of the four corners are provided with R, and the remaining one corner is chamfered.

  Further, a hole 4 is provided, which is designed so that the pattern location can be specified by making the outer shape asymmetric. The material is acrylic resin and the thickness is 2 mm. Grooves are formed on the flow path forming surface, and a sealed flow path is formed by bonding an acrylic film having a thickness of 50 μm. The center of gravity 5 is the center of gravity of the main plate, and a rotating portion fixing hole 3 for fixing the main plate to the rotating portion around the center of gravity 5 is provided.

  Pattern 2 includes a buffer injection port 6 into which a separating DNA conjugate as a buffer is injected, a buffer injection part 7 for temporarily holding the injected buffer, and a sample injection into which a DNA sample is injected. An inlet 8, a sample injection part 9 for temporarily holding the injected DNA sample, a chamber part 16, a sample holding part 20, a buffer part 21, a positive electrode part 12 which is a positive voltage application part, It consists of a negative electrode part 13 which is a negative voltage application part and a flow path connecting them.

  Here, the shape around each part of the buffer injection part 7, the sample injection part 9, the chamber part 16, the sample holding part 20, the buffer part 21, the positive electrode part 12, and the negative electrode part 13 will be described in detail. The buffer injection part 7 and the sample injection part 9 have the same shape, and FIG. 3 shows a sectional view of the periphery. The sample injection port 40 corresponds to the buffer injection port 6 and the sample injection port 8, and the sample injection unit 41 is the buffer injection unit 7 and the sample injection unit 9. The groove 42 corresponds to a flow path extending from the buffer injection part 7 and the sample injection part 9. The non-conductive film 43 is the film described above, and a sealed flow path is formed by pasting the groove 42 so as to cover it.

  The chamber part 16 is connected to the sample injection part 9 by a flow path 15. The sample holding unit 20 is connected to the chamber unit 16 by the flow channel 17 and the flow channel 18. Here, the channel 17 is thinner than the channel 18. In the embodiment, the flow path 17 is 100 μm and the flow path 18 is 200 μm. The buffer section 21 is connected to the chamber section 16 and the sample holding section 20 by the flow path 17, the flow path 18, and the flow path 19, and the buffer section 21 can be vented by the flow path 22. The chamber part 16, the sample holding part 20, and the buffer part 21 have the same shape, and FIG. 4 shows a peripheral sectional view. The holes 44 are portions corresponding to the chamber portion 16, the sample holding portion 20, and the buffer portion 21, and the grooves 45 and 46 are portions corresponding to flow paths extending from the respective portions. Here, the hole 44 is on a vessel having a depth of 1.5 mm when viewed from the flow path forming surface.

  The positive electrode unit 12 is a positive voltage application unit, and the negative electrode unit 13 is a negative voltage application unit. These are connected via a flow path 14, and further connected to the buffer injection part 7 via a flow path 10 and a flow path 11, respectively. FIG. 5 is a sectional view of the periphery of the positive electrode portion 12 and the negative electrode portion 13, and the lower side is a flow path forming surface. The electrode portion 47 is a hole penetrating the plate and corresponds to the positive electrode portion 12 and the negative electrode portion 13, and the grooves 48 and 49 are flow paths extending therefrom. Films are affixed to both surfaces of the plate, and the nonconductive film 43 is affixed to the flow path forming surface side to form a sealed flow path. In addition, a conductive film 50 is attached as an electrode layer on the opposite surface of the flow path forming surface, and a voltage can be applied by pressing the electrode against the conductive film 50.

  Next, the electrode layer will be described in detail. FIG. 6 is a diagram showing a surface opposite to the flow path forming surface of the biological sample discrimination plate in the first embodiment.

  The electrode layer is formed of a conductive film 50. As shown in FIG. 6, the positive electrode contact portion 24 and the positive electrode portion 12 arranged in an arc shape on the outer periphery of the plate are connected on the opposite surface of the flow path forming surface. And a negative electrode layer in which the negative electrode contact portion 25 and the negative electrode portion 13 are connected to each other in a circular arc around the hole in the center of the plate. Therefore, a voltage can be simultaneously applied to all the flow paths by pressing the two electrodes against the positive electrode contact portion 24 and the negative electrode contact portion 25, respectively.

  Here, the case where the electrode layer is formed of a metal film will also be described. FIG. 7 shows a flow path forming surface side of a plate in which an electrode layer is formed of a metal film. The plate has a shape in which a flow path forming plate and an electrode layer forming plate are bonded together, and the flow path forming plate has a circular outer shape, a hole is provided in the center, and the electrode layer is exposed. FIG. 8 is a cross-sectional view of the periphery of the positive electrode portion 12 -positive electrode contact portion 24 and the negative electrode portion 13 -negative electrode contact portion 25. The flow path forming plate 51 is a plate on which the flow path of the pattern 2 described above is formed.

  The electrode forming plate 52 is a plate in which a metal film is applied in the pattern shape of the electrode layer described above, and the electrode layer 53 is a portion corresponding to the positive electrode layer and the negative electrode layer. The electrode portion 54 is a portion corresponding to the positive electrode portion 12 and the negative electrode portion 13, and the groove 55 and the groove 56 are portions corresponding to flow paths extending from them. The electrode contact portion 47 is a portion corresponding to the positive electrode contact portion 24 and the negative electrode contact portion 25 and is connected by an electrode layer. Further, since the electrode contact portion 57 is not covered by either the non-conductive film 43 or the flow path forming plate 51 and is exposed to the outside, a voltage can be applied by pressing the electrode against the electrode contact portion 57. it can.

  Now, an example of specific operations and operations until the presence or absence of SNPs (single nucleotide polymorphisms) in the DNA sample described above will be described with reference to FIG.

  First, a DNA sample as a specimen is prepared.

  Originally, DNA has a double-stranded helical structure, but in the first embodiment, a single-stranded DNA having a length of about 60 bases including a SNPs site to be discriminated is prepared.

  Since the extraction method and single strand formation are not directly related to the present invention, detailed description thereof is omitted.

  Next, a DNA conjugate is prepared as a buffer.

  The DNA conjugate is obtained by covalently bonding a high molecular linear polymer to the 5 'end of a 6 to 12 base long single-stranded DNA. Furthermore, DNA is a sequence that is complementary to the normal type but not complementary to the mutant type, has a strong binding force to the normal type DNA, and a weak binding force to the mutant type DNA. There are characteristics. In addition, when electrophoresed, the linear polymer bonded to the 5 'end has a characteristic that the migration speed is considerably slow.

The “DNA conjugate” described hereinafter has physical properties including a pH buffer that also serves as an electrolyte and a DNA binding force control agent such as MgCl ₂ .

  When sample preparation is complete, the DNA conjugate and DNA sample are injected into the plate. The DNA conjugate is dispensed from the buffer injection port 6 to the buffer injection unit 7 by a pipetter or the like. Similarly, the DNA sample is dispensed from the sample injection port 8 to the sample injection unit 9.

  As for the dispensing amount, although it differs depending on the scale of the pattern, in the first embodiment, the DNA conjugate is 18 microliters and the DNA sample is 2 microliters.

  Next, the plate 1 is fixed to a motor or the like, and the center of gravity 5 is rotated around the shaft. The DNA conjugate and DNA sample dispensed at this time move toward the outer circumference by centrifugal force.

  The DNA conjugate in the buffer injection part 7 is equally divided into the positive electrode part 12 and the negative electrode part 13 through the flow paths 10 and 11, and the DNA conjugate that has moved to the positive electrode part 12 is further quantitatively held through the flow path 14. Move to section 23. Similarly, the DNA conjugate that has moved to the negative electrode portion 13 also moves to the quantitative portion 23 through the flow path 14. FIG. 9A shows a state in which the movement of the DNA conjugate is stopped after 2 minutes from the start of rotation. Moreover, the figure which expanded the fixed quantity holding | maintenance part 23 periphery is FIG.9 (b).

  The DNA conjugate 26 is filled up to about 70% of the positive electrode portion 12 and the negative electrode portion 13, fills the flow path 14, and reaches the quantitative holding portion 23 as shown in FIG. 9B.

  The liquid level height of the DNA conjugate existing in the positive electrode part 12 and the negative electrode part 13 and the liquid level height of the sample quantification part are on the same circumference with the center of gravity 5 as the center.

  Next, the movement of the injected DNA sample will be described with reference to FIGS. The DNA sample in the sample injection section 9 passes through the flow path 15 and reaches the sample holding section 20 positioned on the outer peripheral side through the chamber section 16, the flow path 17 and the flow path 18. However, as shown in FIG. 10, the flow path 18 is connected on the outer peripheral side of the sample holding unit 20, and the sample holding unit 20 has no air vent hole. Therefore, the air remaining in the sample holding unit 20 does not escape and is in a pressurized state.

  FIG. 10 shows the state of the DNA sample 2 minutes after the start of rotation, and 27 and 28 are the DNA samples. Reference numeral 29 denotes compressed air, which maintains a state in which such centrifugal force and applied pressure are in equilibrium only during rotation. In this embodiment, the rotational speed is 4000 rpm.

  Next, the following operation will be described.

The plate 1 is rotated for a certain time, and the rotation is suddenly stopped in a state where the movement of the DNA conjugate and the DNA sample is stopped. (For example, stop from 4000 rpm in 2 seconds.)
Then, since the DNA sample 27 existing in the sample holding unit 20 has lost the centrifugal force, the DNA sample 27 tries to flow backward from the sample holding unit 20 to the flow path 18 by the pressure of the air 29. Further, the DNA sample 26 tries to move to the flow path 17 and the flow path 19 until the air 28 in the sample holding unit 20 reaches atmospheric pressure. At this time, the flow path 17 is formed narrower than the flow path 19. The DNA sample 27 tends to flow more into the channel 19 than the channel 17.

  FIG. 11 shows a state immediately after the sudden stop.

  The DNA sample 27 in the sample holding unit 20 reaches the quantitative holding unit 23 and the buffer unit 21 through the flow paths 18 and 19 due to the expansion of the air 29.

  In particular, the quantitative holding unit 23 comes into contact with the filled DNA conjugate 26.

  At this time, there are some DNA samples that move to the chamber portion 16 through the flow path 17.

  Next, the plate 1 is rotated again for a few seconds at medium speed. At this time, it is important to moderate the deceleration.

  The state after the stop is shown in FIG.

  Although the DNA sample filled in the flow path 19 flows to the chamber part 16, a small amount of DNA sample remains in the quantitative holding part 23. The remaining DNA sample is electrically insulated from other DNA samples.

  The reason why the operation is different from the first rotation is that the rotation speed is slow and the deceleration is slow, so that the air 29 in the sample holder 20 is not strongly compressed. Therefore, it does not flow backward and the channel 19 is not filled again.

  With the above operation, the sample DNA remaining in the quantitative holding unit 23 becomes the final sample for determining the SNPs.

  Next, electrophoresis is performed. In electrophoresis, a positive electrode is pressed against the positive electrode contact portion 24 and a negative electrode is pressed against the negative electrode contact portion 25, and a voltage of several hundred volts is applied while rotating the plate. Then, an electric field is generated in the flow path 14 and the quantitative holding unit 23, and the DNA sample remaining in the fixed quantity holding unit 23 moves in the flow path 14 to the positive electrode side (A direction in FIG. 13).

  Here, since the DNA conjugate moves by centrifugation and is filled in the flow path 14 and the positive electrode portion and the negative electrode portion, the DNA conjugate is held biased toward the outer peripheral portion of the plate when a voltage is applied. It is in contact. Therefore, a voltage can be applied reliably.

  Moreover, since the positive electrode contact part 24 and the negative electrode contact part 25 are connected with the positive electrode part 12 and the negative electrode part 13 of all the flow paths through the electrode layers, respectively, the two electrodes are respectively in positive electrode contact. By pressing against the portion 24 and the negative electrode contact portion 25, a voltage can be applied to all the channels simultaneously, and electrophoresis can be performed in eight channels at the same time.

  In addition, since the positive electrode contact portion 24 and the negative electrode contact portion 25 are arranged in concentric circular arcs, there is a difference in the voltage applied to each flow path depending on where the electrodes are in contact with each other. By applying the voltage, it is possible to apply a voltage equally to all the flow paths, and to perform electrophoresis stably.

  By the way, when performing electrophoresis, even if a voltage is applied without centrifugation, the current value that flows through the electrode layer is very small. The loss becomes very small, and a voltage drop applied to each flow path hardly occurs. Therefore, there is no big trouble in performing electrophoresis. However, in order to perform electrophoresis more stably and reliably, it is preferable to apply a voltage while rotating the plate.

  The channel 14 is filled with a DNA conjugate, and the DNA sample is electrophoresed while repeatedly binding to the DNA conjugate. At this time, as described above, the normal DNA in the DNA sample has a strong binding force with the DNA conjugate, so the migration speed is slow, and the mutant DNA has a weak binding power, so the migration speed is faster than the normal type. That is, when both the normal type and the mutant type are present in the DNA sample, the normal type DNA and the mutant type DNA are separated, and SNPs can be discriminated.

  FIG. 14 is a graph obtained by scanning DNA during electrophoresis in the detection unit 30 which is a part of the flow path 14 shown in FIG.

  The DNA was detected by exciting the DNA modified with a fluorescent label (FITC) with light at 470 nm and detecting light at around 520 nm. DNA may be detected by absorbance at 260 nm.

  The horizontal axis represents the position of the detection unit 30 and DNA migrates from left to right. That is, the left side is the quantitative unit 23 and the right side is the positive electrode unit 12 side. The vertical axis represents the fluorescence intensity, and represents a waveform that changes in time every minute from the top. You can see the two mountains gradually separating. In this case, it was determined that the same amount of normal DNA and mutant DNA were present in the DNA sample.

  The peak on the right side of the graph is the mutant type because the migration speed is fast, and the left side is the normal type because the migration is slow.

  As described above, according to the first embodiment, by providing the sample holding unit 20, a fixed amount of DNA sample is held in the quantification unit 23 on the plate 1 only by the rotation operation, and the positive electrode unit 12 and the negative electrode unit 13 and the flow path 14 were configured to allow electrophoresis. Furthermore, the flow path 14 for electrophoresis has an arc shape, so that an extremely accurate detection result can be obtained when a DNA sample obtained by extracting specific DNA from cells, blood, or the like is measured on the biological sample discriminating apparatus plate. It becomes possible.

  The plate for a biological sample discriminating apparatus according to the present invention is useful as one that can discriminate a biological sample such as a DNA sample at low cost and simply.

The figure which shows the flow-path formation surface of the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. The figure which shows the pattern formed in the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. Sectional drawing of the sample injection part of the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention, and a buffer injection part Sectional drawing of the chamber part, sample holding | maintenance part, and buffer part of the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. Sectional drawing of the positive electrode part and negative electrode part of the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. The figure which shows the electrode layer formation surface of the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. The figure which shows the flow-path formation surface side when the electrode layer of the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention is formed with the metal film. Sectional drawing of the plate in case the electrode layer of the plate for biological sample discrimination | determination apparatuses concerning the form 1 of visual inspection of this invention is returned with a metal film The figure which shows the state of a DNA conjugate when the sample filling process is given to the plate pattern for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. Quantification part enlarged view which shows the state of DNA conjugate when the sample filling process is performed to the plate pattern for biological sample discriminating apparatus according to the first embodiment of the present invention. The figure which showed the state of each sample currently filled with the DNA conjugate and the DNA sample in the plate for biological sample discrimination devices concerning Embodiment 1 of this invention, and rotating at 4000 rpm. The figure which showed the state of each sample when it fills with the DNA conjugate and the DNA sample in the plate for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention, and it stops suddenly after rotating at 4000 rpm. The figure which showed the state of each sample immediately after re-rotating after filling the DNA conjugate and the DNA sample in the biological sample discrimination device plate concerning Embodiment 1 of this invention, stopping rotation at 4000 rpm. The figure which showed the part which performs the DNA scan of the plate pattern for biological sample discrimination | determination apparatuses concerning Embodiment 1 of this invention. The quantitative sample formed on the biological sample discriminating device plate according to the first embodiment of the present invention is filled with the DNA sample, and the DNA sample moves through the separation DNA conjugate filled in the first channel. Figure showing how to do

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Plate 2 Flow path pattern 3 Rotating part fixing hole 4 Positioning hole 5 Plate center of gravity 6 Buffer injection port 7 Buffer injection port 8 Sample injection port 9 Sample injection port 10, 11 Flow channel 12 Positive electrode portion 13 Negative electrode portion 14 , 15 Channel 16 Chamber portion 17, 18, 19 Channel 20 Sample holding portion 21 Buffer portion 22 Channel 23 Flow rate holding portion 24 Positive electrode contact portion 25 Negative electrode contact portion 26 DNA conjugate 27 DNA sample 27 DNA sample 28, 29 Air 30 Detection unit 40 Sample injection port 41 Sample injection unit 42 Groove 43 Non-conductive film 44 Hole 45, 46 Groove 47 Electrode unit 48, 49 Groove 50 Conductive film 51 Channel formation plate 52 Electrode formation plate 53 Electrode layer 54 Electrode unit 55 56 Groove 57 Electrode contact area

Claims (5)

In the plate for a biological sample discriminating apparatus provided with a plurality of channels for detecting a transport reaction obtained by moving a biological sample by electrophoresis in a buffer and discriminating the biological sample, each of the plurality of channels A pair of electrodes is provided in the flow path, and one electrode of the pair of electrodes is electrically connected to an electrode provided at a central portion of the biological sample discriminating apparatus plate, and the other of the pair of electrodes Are electrically connected to the electrode provided on the outer peripheral portion of the biological sample discriminating apparatus plate, and the electrode provided in the central portion of the biological sample discriminating apparatus plate and the outer peripheral portion of the biological sample discriminating apparatus plate A biological sample discriminating apparatus plate, wherein the biological sample is electrophoresed in a buffer based on a potential difference with an electrode provided. The said electrode consists of a conductive film or a metal film, The plate for biological sample discrimination devices of Claim 1 characterized by the above-mentioned. 2. The biological sample discriminating apparatus plate according to claim 1, wherein the conductive film is affixed to a surface opposite to a flow path forming surface of the biological sample discriminating apparatus plate. The biological sample discriminating apparatus according to claim 2, wherein the metal film is sandwiched between a plate in which no flow path is formed and a surface opposite to the flow path forming surface of the plate in which the flow path is formed. Plate. The said metal film is either copper, aluminum, or gold | metal | money, The plate for biological sample discrimination | determination apparatuses in any one of Claim 2 or Claim 4 characterized by the above-mentioned.

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