JP2006223280A - Method for culturing photosynthetic bacterium with stevia and cultured aqueous solution - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for culturing by which a gas is scarcely generated in a fermentation process by carrying out fermentation of photosynthetic bacteria at normal temperature under atmospheric pressure with a transparent (an acrylic, glass, etc.) water tank and to provide a cultured aqueous solution. <P>SOLUTION: The photosynthetic bacteria are cultured in a culture solution prepared by fermenting stevias with the photosynthetic bacteria to produce an aqueous solution of the photosynthetic bacteria having mutual characteristics of the photosynthetic bacteria and stevias. Thereby, the method for culturing the photosynthetic bacteria having the characteristics of natural regression possessed by the photosynthetic bacteria and various excellent actions of the stevias in combination and the cultured aqueous solution are provided. The aqueous solution has remarkable effects as compared with those of conventional photosynthetic bacteria such as degrading actions on malodors, antioxidizing actions and degrading actions on sewage sludge, etc. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for culturing photosynthetic bacteria using a culture solution in which stevia (Asteraceae native to Paraguay, etc.) is added in advance to the photosynthetic bacteria and fermented, and the cultured aqueous solution.

Conventionally, photosynthetic bacteria are widely distributed on the earth and use solar energy to perform photosynthesis, and in the natural environment, carbon dioxide assimilation and its reverse decarboxylation, nitrogen fixation and its reverse denitrification, oxidation of sulfide, Photosynthetic bacteria involved in reduction play a major role in the circulation of carbon, nitrogen and sulfur on the earth, and are widely used as beneficial microorganisms in the fields of agriculture, livestock, sewerage, and industry.
In the field of agriculture, it is partially used for organic cultivation of paddy fields and field crops for the purpose of controlling food diseases, improving quality, and decomposing residual agricultural chemicals to remove harmful substances.

  In the field of livestock farming, in the case of livestock farmers, it removes malodors by spraying them on livestock and barns, promotes fermentation of compost, treats manure, etc., and reduces the malodor of manure by feeding chickens as food. Since it decreases and becomes healthy, the use of drugs can be refrained, and it is used because it increases the survival rate when used for increasing egg-laying rate, improving egg quality, and fish farming.

  In the field of sewerage, it is used for the removal of odors from settlement wastewater treatment plants, sewage treatment plants, and sludge decomposition treatment.

  In the industrial field, it has long been used for fermentation of miso, soy sauce, sake, and the like. It is also used for purposes such as yogurt, health and environmental conservation (production of biodegradable plastics).

  Regarding the cultivation of photosynthetic bacteria, anaerobic digestion method of organic waste water and organic waste utilizing light and a method of producing photosynthetic bacteria (Patent Publication 2000-153292 public relations), culture for 10 liters of water-soluble basic culture medium Ratio culture added water Tap water containing 0.1PPm to 0.3PPm Photosynthesis bacteria culture method (patent publication 2000-287675), rice squeeze that is cultivated by UV sterilization (exclusion of airborne germs) for 24 hours per culture under open air A method of culturing photosynthetic bacteria using a broth fermented with fermented bacteria as a culture solution (Patent Publication 2002-171963), stirring the culture solution in the container, sterilizing briefly by heating and boiling, and then inoculating the seeds of the photosynthetic bacteria into the container Manufacturing methods such as a method of culturing while stirring in a vessel (Koh Publication No. 11-243946) are currently available.

  As described above, in conventional culture apparatuses for photosynthetic bacteria, lactic acid bacteria, yeast, and the like are used in the culture solution and gas is generated. Therefore, pressure-resistant containers are used, and complicated apparatuses such as pressure reducing valves and stirring control devices are used. is necessary. In addition, when a pressure resistant container or the like is not used, a management control method such as heating, stirring, and temperature management of a mixed solution with photosynthetic bacteria is used. For this reason, production costs are high and it is difficult to culture at low cost.

Problems to be solved by the invention

  In order to contribute to the solution of multipurpose problems related to agriculture, livestock, environmental conservation, etc., the present invention provides a water tank with transparent (acrylic, glass, etc.) water-soluble culture solution after fermenting stevia powder with photosynthetic bacteria. It is an object of the present invention to provide a method of culturing without causing gas generation during the culturing process and a cultivated aqueous solution by culturing at room temperature at atmospheric pressure.

Means to solve

  In the use of photosynthetic bacteria used in agriculture, environmental conservation, etc., the present invention is about organic farming, removal of odors generated by garbage compost, purification of odors / water in sewage treatment plants, decomposition of sludge, etc. As a result of research to find immediate effects and to cultivate strong photosynthetic bacteria, it was noted that stevia, which is used as a sweetener, has recently been used as an agricultural material in the cultivation of agricultural products. In this study, Stevia powder was first fermented with photosynthetic bacteria and then used as a culture solution to study the method of culturing photosynthetic bacteria.

  The photosynthetic bacteria aqueous solution cultivated by this method has a number of stevia effects, which have the characteristics of the original photosynthetic bacteria and the effects on the pests of stevia, and 4-5 times the antioxidant properties of green tea. It appeared remarkably in the aqueous solution.

  Stevia powder as an agricultural material is decomposed by soil bacteria after spraying and absorbed into agricultural products. At this time, the growth of Azobacter, which is a bacterium that fixes nitrogen under aerobic conditions, and a photosynthetic bacterium that performs photosynthesis under anaerobic conditions, are not so good. However, when they are mixed and cultured, the growth of each other is increased several times, sometimes tens of times. By testing these and adding stevia powder to the culture solution of photosynthetic bacteria, stevia powder is decomposed and absorbed by photosynthetic bacteria, so when sprayed directly on agricultural products, it is not necessary to obtain mediation of soil bacteria etc. Because it acts on roots and leaves, it has an immediate effect on agricultural products.

  Originally, nutrients in soil are decomposed by soil fungi and taken into food, but in recent years, the decrease in soil fungi due to the use of chemical fertilizers has become a basic disorder such as nitrate and nitrite in food. The aqueous solution is a photosynthetic bacterial aqueous solution that can cope with these base disorders.

  A photosynthetic bacterium conventionally has an antioxidant effect. In the photosynthetic bacterium of the present invention, synergistically with the antioxidant effect of stevia, a strong antioxidant effect is produced, and nitrate nitrogen in soil is fixed and decomposed.

  This is because the photosynthetic bacterium of the present invention decomposes hydrogen sulfide, ammonia, etc. in the soil, which is currently a problem in the agricultural field, so that actinomycetes proliferate, reduce the influence of nitrate, and prevent the decay of organic compost be able to. There is also a synergistic effect with stevia, and it has the function of supplying roots with inorganic salts such as nitrogen, phosphate, and potassium absorbed by food from the soil and increasing the water absorption capacity of the roots, extending the life of the hair roots. It helps protect seedlings from heavy metal poisoning and is used as a soil conditioner to increase yield and improve continuous soil.

  In the environmental field, the deodorizing effect of photosynthetic bacteria is well known, but the photosynthetic bacteria of the present invention were used in the fallen sewer of Iitate Village, Fukushima Prefecture for the purpose of stabilizing water quality and reducing sludge in April 2004. As a result, the amount of sludge generated is reduced by half in 4 months, and the odor in the treatment plant is reduced. Similarly, when the photosynthetic bacterium of the present invention is used as an odor countermeasure for the whole facility of the Iwase Village Rural Fall Water System in Fukushima Prefecture, the odor is reduced in two weeks. From this, in addition to the decomposition of sewage sludge, deodorant, bactericidal action of harmful bacteria, pet deodorant, nursing facility deodorant, livestock facility deodorization and manure compost adjuvant, sewerage, livestock, Used in fields such as nursing care.

  Since the photosynthetic bacterium of the present invention has a synergistic effect with stevia and has a stronger antioxidant effect than general photosynthetic bacteria, in addition to the decomposition of nitrates in the agricultural field, cement can be used as a neutronization inhibitor for reinforced concrete. It can be used as an admixture.

  This can also be used for underwater concrete, revetment blocks, environmental blocks, etc., and because it has a strong ability to decompose salt, it can be caused by salt damage cultivated land on the Sea of Japan side or tsunami caused by the Sumatra earthquake. It has high utility value as a soil conditioner for salt-harm agricultural land in Southeast Asia.

  Thus, by using the low-cost photosynthesis bacteria, the effects of the photosynthesis bacteria and stevia, such as bactericidal effect, detoxification, and dioxin decomposition, are exhibited by the main culture and can be used in various fields.

The present invention has resulted in the present invention as a result of repeated studies to solve the above problems. A method for culturing photosynthetic bacteria using a transparent (acrylic and glass) water tank at room temperature will be described below.
The method for cultivating photosynthetic bacteria according to the present invention is a method for accumulating and cultivating photosynthetic bacteria using a culture medium obtained by stirring and cultivating a powder of stevia (Asteraceae perennial plant native to Paraguay, South America) in a rice field or the like. In other words, the effects of Stevia are improved by synergistic effects with photosynthetic bacteria. This section describes the various effects of Stevia that have been confirmed to be useful.
“JBB Agrisystem” in-house study material, provided by Stevia Laboratories, Inc. JBB Stevia fruit and vegetable test results Increases sugar content and nutrients (sugar, vitamins, minerals, enzymes)
2. Bactericidal action of harmful bacteria (food poisoning bacteria such as O-157, Salmonella, verotoxin)
3. Virus suppressive action (AIDS HIV virus, O-Esky virus, etc.)
4). Inhibition of harmful substances and environmental hormones (organophosphorus, nicotine degradation, dioxin 96% degradation)
5. 5. Preventing allergy and atopy 6. Histamine detoxification, steroid side effect control action Rooting promoting action8. Normalizing action of reproductive hormones9. Activation of useful microorganisms (bifidobacteria, lactic acid bacteria, etc.)
10. The present invention will be described in detail below, in which the shelf life of fruit and vegetables is extremely improved by the antioxidant action.

Place a transparent aquarium container in a place where the sun is well (lighted place), put 20 liters of the original photosynthetic bacteria, add 20 grams of stevia powder and stir. 20 liters of the following culture solution is added thereto to make a total of 40 liters, and the surface is sealed with a transparent vinyl sheet. Completion when the aqueous solution in the aquarium changes to the color (proliferates) of conventional photosynthetic bacteria.
The photosynthetic bacteria in the aquarium become the original photosynthetic bacteria color (growth) in 1-2 days in summer, and grow in 5-7 days with temperature adjustment of 6 degrees or more in winter.
In the production method, 50% of photosynthetic bacteria and 50% of a water-soluble culture solution are mixed, and the step of applying lighting (ceal light) is repeated.
Cultivation ratio for 100 liters of water-soluble culture solution Culture-added water-ground water (if it does not contain chlorine) 100 g ammonium chloride, 100 g sodium hydroxide anhydrous, 100 g sodium bicarbonate, 100 g natural salt, 100 stevia powder Gram, dipotassium hydrogen phosphate 20 grams magnesium sulfate 20 grams sodium propionate 20 grams peptone 20 grams yeast extract 10 grams DL-malic acid 25 grams

  This figure shows how to cultivate photosynthetic bacteria using Stevia medium. First, (1) 20 grams of stevia powder is mixed with 20 liters of photosynthetic bacteria and fermented (cultured). (2) Mix in the same ratio as 20 liters of the previously prepared culture solution. (3) A total of 40 liters of photosynthetic bacteria culture solution will be produced. On summer days, cultivation is completed in 24 to 48 hours. (4) Half of this culture solution is used for the finished product and the other half is used for fermentation (culture).

Claims (3)

A method of culturing photosynthetic bacteria in a culture solution in which stevia is fermented with photosynthetic bacteria and an aqueous solution obtained by culturing A process and a culture method of culturing the aqueous solution of claim 1 in a fermented solution of photosynthetic bacteria and stevia at room temperature using a water tank transparent (acrylic, glass, etc.) at atmospheric pressure The aqueous solution cultured in claim 1 is synergistic with photosynthetic bacteria and Stevia's unique action, and has significant effects on odor, antioxidant, sewage sludge decomposition, etc., compared to conventional photosynthetic bacteria. How to use in all fields

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