JP2006204296A5 - - Google Patents

Claims (30)

Human intestinal bacteria and strain-specific primers that have the ability to assimilate daidzein to produce equol, or the intestines that improve the ability to assimilate daidzein in human gut microbiota and produce equol Bacteria inside. The intestinal bacterium according to claim 1, which is a species-specific primer of the intestinal bacterium and can be selectively detected by a part of the sequence shown in SEQ ID NO: 3. The enteric bacterium according to claim 1, wherein the enteric bacterium has a daidzein assimilation ability of 1.0% or more measured after 24 hours using daidzein as a substrate. The enteric bacterium according to claim 1, wherein the enteric bacterium has a daidzein assimilation ability of 10% or more measured after 24 hours using daidzein as a substrate. The enteric bacterium according to claim 1, wherein the enteric bacterium has a daidzein assimilation ability of 20% or more measured after 24 hours using daidzein as a substrate. The enteric bacterium according to claim 1, wherein the enteric bacterium has a daidzein assimilation ability of 30% or more measured after 24 hours using daidzein as a substrate. The intestinal bacteria is characterized by having Ecole productivity imparting effect against the Ecole non-producing human flora, intestinal bacteria of claim 1. The intestinal bacteria is characterized by having Ecole productivity improvement of more than double for the Ecole productivity human flora, intestinal bacteria of claim 1. The intestinal bacteria, in the National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary, or was deposited as a consignment number FERM P-20729 No. (commissioned Date 2005 December 2, 2009), accession number FERM P- 20325 No. (entrusted Jan 2004 December 16) or deposited as, or RIKEN is stored strain of microorganism strain preservation facility Bifidobacterium breve JCM 1273 in Enterobacteriaceae according to claim 1. The intestinal bacterium according to claim 1, which is a bacterium usable as a probiotic. The intestinal bacterium according to claim 1, further having an ability to assimilate starch or glycogen. A plurality of combinations of enteric bacteria according to claim 1 . 13. A combination of enteric bacteria according to claim 12 . To improve the Ecole producing ability, the composition comprising a combination according to any one of claims 1 to 1 1 in Enterobacteriaceae or claim of any one 1 2-13. A food or a pharmaceutical composition according to claim 1 4. Further comprising other active ingredients, the composition according to claim 1 4. The intestinal bacteria are provided in solution in a liquid composition according to claim 1 4. The intestinal bacteria are provided in solid form, the composition according to claim 1 4. The intestinal bacteria are provided lyophilized composition according to claim 1 4. The enterobacteria further comprising a component to be protected from gastric juice composition according to claim 1 4 A method for improving the Ecole producing ability, claim 1 to 1 1 Enterobacteriaceae according to any one, a combination according to any one of claims 1 2 to 13, or claim 1 121. A method comprising administering the composition of any one of 4 to 120 to a subject in need of treatment. A method for producing a bacterium for producing equol by assimilating daidzein, having a daidzein assimilation ability or improving equol production ability, wherein a sample expected to contain the bacterium is obtained using an EG medium A method comprising the step of culturing. A method for producing human intestinal bacteria or human intestinal bacteria that has an ability to assimilate daidzein to produce equol and that enhances the ability to produce equol,
A) providing a sample containing human intestinal derived material or feces;
B) culturing the sample at 37 ° C. using EG medium;
C) brain heart infusion 37 g, agar 1 g, L-cysteine hydrochloride 0.5g, dissolved by heating the sodium carbonate 4g in 1 liter of distilled water, was dispensed in a test tube while blowing oxygen-free carbon dioxide, Bed Suspend the solution in 0.2 ml of the reaction solution prepared by autoclave sterilization with a chill rubber stopper, and add 1 μl (20 mg / ml) of daidzein to the suspension so that the bacterial concentration is about 10 ⁹ / ml And then incubating with a simple anaerobic culture apparatus Aneropack at 37 ° C. for 24 hours;
D) a step of analyzing isoflavones in the reaction solution; and E) a method comprising selecting and producing enterobacteria having the ability to assimilate daidzein or its derivatives to produce equol. The sample is a sample derived from a healthy human feces, the method according to claim 2 3. Bacteria prepared by the method according to any one of claims 2 3 21 to 24. A method for producing or medicament for improving the Ecole productivity including equol, or enteric bacteria or claim 1 2-1 3 according to any one of claims 1 to 1 1 1 A method comprising mixing the combination according to paragraph and daidzein, and incubating the mixed mixture under conditions that the enteric bacteria assimilate. The combination according to any one of the Enterobacteriaceae or claim 1 2-1 3 according to any one of claims 1 to 1 1, step mixing the daidzein, and the mixed mixture A composition produced by a method comprising the step of incubating under conditions in which enterobacteria are assimilated. Bacteria obtained by the method of claim 26 . For the manufacture of a medicament comprising equol, enteric bacterium according to any one of claims 1 to 1 1, either bacteria or claim 1 2-1 3 obtained by the method of claim 27 Use of a combination according to paragraph 1. For producing a medicament for improving the Ecole producing ability, claim 1 to 1 1 Enterobacteriaceae according to any one, bacterial or claim 1 2 obtained by the method of claim 27 to Use of the combination according to any one of 1 to 3 .