JP2006153656A - Reaction capsule, inspection method using it and inspection system - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a reaction capsule which is used in the reaction in a liquid and enables the enhancement of reaction efficiency. <P>SOLUTION: The reaction capsule is constituted so as to perform reaction in the liquid and equipped with a receptor for recognizing a target substance and a detection and reaction part for performing the reaction necessary for detection on the basis of the recognition data from the receptor. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a reaction capsule for detecting a trace substance related to a biological substance, and an inspection method and an inspection system using the same. The present invention is preferably applied to medical treatment, and is applied to in-vitro diagnosis or in-vivo diagnosis of an apparatus and method for performing a test of a biological substance in blood, specimen fluid, or tissue.

  The use of bioactive substances consisting of biological materials or non-biological materials that can act on living organisms has come to provide important benefits not only for medical treatment but also for a wide variety of industries. When these biological materials and biological substances including biologically active substances are used, biological reactions according to the purpose of use are involved. Biological reactions are carried out in the liquid state and the main reaction components have been prompted to come into direct contact with the liquid or to mix with each other. A biological reaction using microparticles having an average diameter of 100 μm or less as a reaction component also includes a reaction between a drug ejected from the particle or a reagent coated on the particle surface and a liquid component. On the other hand, there is a case where a liquid biological reaction is performed in a closed reaction vessel, such as a lab-on-a-chip. All of the reaction components are introduced and mixed in the closed reaction vessel, whereby all biological reactions are carried out in the reaction vessel (see Literature 1 and Literature 2). In recent years, a technique for performing various functions in a cell by injecting a drug or reagent as a reaction component into the cell has also been reported.

  On the other hand, in medical treatment, improvement of QOL (Quality Of Life) by minimally invasive endoscopy such as capsule endoscopes, and introduction of SNP (Single Nuculeotide Polymorphism) typing to the genetic characteristics of individuals. EBM (Evidence Based Medicine) based on so-called “individual medicine”, which selects appropriate preventive and therapeutic methods, is about to be transformed as a new concept. Here, words such as POCT (Point Of Care Testing) have also been made in conventional biochemical tests, and the responsiveness and ease of testing are being sought.

Capsule endoscopes allow observation of areas that could not be seen with conventional endoscopes, and the patient's painful biochemical tests when swallowed or inserted mainly collect the subject's blood with a syringe. Is painful, and it is impossible to conduct tests at home.
JP-A-11-75812 JP2001-153860

Thus, the use of conventional biological materials has caused biological reaction pairs (eg, sample and reagent) to exist in the same liquid environment. Biological substances mixed in the same liquid may cause harmful effects on living things such as the global environment and human body. Tools for introducing reaction components into a closed reaction vessel also need to consider environmental pollution. Therefore, methods and means for carrying out reactions without mixing reaction pairs for biological reactions in liquid form are the main object of the present invention. At other angles, methods and means for performing a closed reaction without the introduction of reaction components by tools are another object of the present invention.

  Another object of the present invention is to provide a reaction reagent and method capable of detecting a fluid, particularly a sample containing a biological substance, with high sensitivity and applicable to in-vivo diagnosis, and an apparatus for detection. .

  The present invention includes the contents listed in the following items.

  The reaction capsule of the present invention is a reaction capsule that performs a reaction in a liquid, and includes a receptor that recognizes a target substance and a detection reaction unit that performs a reaction necessary for detection based on recognition information from the receptor.

  The reaction method using the reaction capsule of the present invention includes a step of bringing a liquid containing a target substance into contact with the reaction capsule, and a step of starting another reaction in the capsule in conjunction with recognition of a receptor provided in the reaction capsule. It comprises.

  In addition, the inspection system using the reaction capsule of the present invention includes a measurement unit that selectively measures the reaction result in the reaction capsule in a liquid environment including the reaction capsule. Preferably, a display unit for displaying data of the measurement unit is further provided. Preferably, an analysis unit that analyzes data of the measurement unit and a determination display unit that displays a determination result of the analysis unit are further included.

In the reaction capsule of the present invention, the reaction required for detection is performed in the capsule that does not come into direct contact with the liquid to be reacted, so that the reaction efficiency is remarkably improved. In addition, since the reaction component does not flow out of the capsule only inside the capsule but can be discharged or decomposed together with the capsule, there is an effect of effectively reducing body contamination or natural environment pollution. The reaction method of the present invention comprises a step of bringing a reaction capsule into contact with a liquid containing a target substance, and a step of starting another reaction in the capsule in conjunction with recognition of a receptor provided in the reaction capsule, Since a large volume reaction in an open environment outside the capsule is converted into a small volume reaction in a closed environment, the reaction efficiency can be maximized. Moreover, since the test | inspection system of this invention selectively measures the reaction result in a reaction capsule, it is effective in monitoring the reaction in the capsule derived | led-out from the liquid environment.

  The reaction capsule and inspection system of the present invention will be described below with reference to the drawings, including methods and apparatuses. However, the present invention is not limited to the following specific description, and includes various modifications and equivalents within the technical scope of the gist of the invention and the claims. Further, as a technique for realizing the embodiment of the present invention, various advanced and known techniques such as DDS (drug delivery system), FCM (flow cytometer), artificial cell, etc. are referred to for the present invention. It is possible to improve the combination.

In the present specification, the “biologically related substance” includes not only cells of animals, plants, microorganisms and the like, but also substances derived from viruses and the like that cannot grow by themselves unless they are parasitic on them. Biologically relevant substances include not only natural forms directly extracted and isolated from these cells, but also those produced using genetic engineering techniques and those chemically modified. More specifically, hormones, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids and the like are included. In addition, non-biological materials that cause a biological reaction to any of the aforementioned biological materials are also included in the “biologically related substance” of the present invention. For example, allergens that are non-biological organic or inorganic substances that induce allergic reactions to biological materials, biological reaction disruptors that induce hormonal action by misrecognition (for example, environmental hormones), and biological materials Any chemical (eg bactericides, photocatalysts, antibacterial materials, carcinogens)
Is mentioned.

  In the present invention, the “biological reaction” may mean any reaction caused by the biological substance to be used. Major reactions include biochemical reactions (eg, enzyme metabolism reactions, enzyme-catalyzed reactions), immunological reactions (eg, antigen-antibody binding reactions, antibody production reactions, histocompatibility reactions), nucleic acid reactions (eg, base sequence complementary binding) , Polymerase reaction, ligase reaction, RNA interference reaction). In some cases, a substance called “probe” is used as a reagent for performing a biological reaction, and “probe” means a substance that specifically binds to the above-mentioned biologically relevant substance. Any one of a ligand and its receptor, an enzyme and its substrate, an antigen and its antibody, a nucleic acid having a specific sequence and a nucleic acid having a complementary sequence thereto, and the like are included. A “reaction pair” in a biological reaction refers to at least two or more substances that react with each other, and it is not necessary for all of the reaction pairs to contain biological material.

First Embodiment A form in which a receptor, a protein necessary for signal transmission, and a cell-free protein synthesis system are incorporated into erythrocytes FIG. 1 is a sectional view showing a reaction state by a reaction capsule imparted with reactivity to erythrocytes.
In this embodiment, a reaction capsule with erythrocytes as the outer shell is provided. The blood cell membrane 1 constituting the outer shell of red blood cells functions as a target recognition unit that specifically recognizes a target substance to be detected. In order to function as a target recognition unit, as shown in detail in the figure, a recognition receptor 2 is installed on the red blood cell membrane. This receptor extends inside the blood cell membrane 1. A reaction solution 3 that functions as a detection reaction unit including a reaction component for performing a reaction necessary for detection is accommodated inside the blood cell. The reaction solution 3 preferably also includes a transmitter 4 that receives target recognition information from the receptor and prompts the start of the reaction. The main reaction component of the detection reaction is the reaction component of the signal protein synthesis system (for example, fluorescent protein, photoprotein, etc.) required for signal transmission that enables measurement from outside the capsule upon receiving a signal from the transmitter. 5 (for example, a synthetic enzyme, a protein raw material for synthesis, etc.). The receptor fully contacts an external liquid component on the surface of the blood cell membrane 1, recognizes the target substance 6 that is a reaction pair in the liquid by the receptor 2, and sends out a substance that transmits the recognition inside the erythrocyte. Various reactions such as signal transduction and other protein synthesis are performed inside the erythrocyte. When a reaction inside the erythrocyte occurs, it can be detected by an appropriate measuring means (for example, optical measurement, electric field measurement, magnetic force measurement, etc.). In addition, various measurements (for example, quantification of the amount of hormone, etc.) are possible by selecting a receptor according to the purpose from among a wide variety of receptors. The technique for processing natural red blood cells or artificial red blood cells can use various known ones related to artificial blood for blood transfusion, and will not be described in detail. It is best to use your own red blood cells. The blood cell membrane 1 blocks the reaction solution 3 inside the blood cell from mixing with an external liquid, and ensures a closed reaction inside the blood cell.

Second Embodiment A form in which a receptor and a cell-free protein synthesis system are incorporated into liposome particles In FIG. 1, in the first embodiment, erythrocytes are used as a capsule shell having a target recognition part, but instead, a diameter of 2 to 4 μm is used. Other than that, the above-described receptor 2 and the reaction component 5 of the cell-free protein synthesis system as the detection reaction part are similarly provided. In this case, it is possible to provide a safe reaction capsule that is easy to manufacture and performs the same function as the reaction capsule made of red blood cells.

Third Embodiment A form in which a receptor and a cell-free protein synthesis system are incorporated into artificial particles made of a semipermeable membrane. FIG. 2 is a cross-sectional view showing the structure of a reaction capsule in which reactivity is imparted to the semipermeable membrane 7.
This is the same as the first embodiment except that a semipermeable membrane 7 such as cellulose or acetate is used as the outer shell. In this case, the semipermeable membrane 7 does not have a receptor, but instead allows only a small amount of a specific component necessary for the reaction from the external liquid to pass through the capsule. Thus, a direct and closed biological reaction consisting of a specific component permeated within the capsule and the reactive component 5 can be carried out.

Fourth Embodiment A form in which a cultured cell or a single cell organism genetically modified for fluorescent protein synthesis is used as a reaction capsule In this embodiment, a living cell or an artificial cell is used as a reaction capsule. Functions such as protein synthesis and substance detection possessed by (for example, nuclei, mitochondria, endoplasmic reticulum) can be directly used for desired reactions in the capsule. Since the cell has all the elements of the outer shell, receptor, transmitter, and synthesis system, the closed reaction can be executed quickly and inexpensively by making the best use of the cell function. In order to convert the target substance present in the liquid outside the cell into a reaction in the cell, at least the receptor is designed according to the target substance.

Fifth embodiment Form using molecular beacon as a fluorescent substance in reaction capsule By using molecular beacon, signal generation and special protein synthesis are advanced only when it reacts with a specific reaction component in the capsule. Is possible.

Sixth Embodiment A form in which a DNA computer is used in a reaction capsule According to Israel's Shapiro et al., Another reaction is started only when a reaction occurs in a specific combination based on the logical operation function of the DNA computer. A method that can be controlled is disclosed. Therefore, by causing the reaction capsule to perform various types of molecular reactions simultaneously and in parallel, the logical operation and the separate reaction (for example, synthesis of the identification signal substance for measurement) are performed, so that a required reaction can be performed in one reaction capsule. The combination of reactions can be discriminated at high speed and autonomously. For the technology relating to the DNA computer, reference can be made to, for example, JP-A-2002-318992.

Seventh Embodiment FIGS. 3 and 4 show an example in which a reaction capsule is applied to a circulation system.
The reaction capsule described in each of the above-described embodiments can be used in a blood vessel as an internal circulation system or a circulation flow channel as an extracorporeal circulation system.
FIG. 3 shows a flow path as a circulation system element that circulates the reaction capsule 11, a drive pump 13 that circulates and drives the flow path 12, and a flow path measurement arranged in the vicinity of a part of the flow paths 12. Display unit 14, analysis unit 15 that analyzes measurement data from the flow channel measurement unit 14, and display that displays analysis results from the analysis unit 15 that analyzes real-time time-series data and / or measurement data from the measurement unit 14 Part 15. The analysis unit 15 and / or a control unit that performs control related to a series of operations such as pump driving, measurement, data processing, and display are included. The analysis result by the analysis unit 15 is different from the measurement data itself, and the final determination result for a desired inspection item can be output to the display unit 16. The display unit 16 may have a plurality of display portions for displaying a plurality of types of display contents as necessary. The display format may be any combination of various marks, numerical values, graphs, symbols, and colors that are easy to see. An additional function of the display unit 16 may be accompanied by a voice or paper printing function.

  FIG. 4 is a modified example of FIG. 3, and a hollow shape that can flow along the movement trajectory in which the liquid 18 containing the reaction capsule 11 and the target substance circulates instead of the flow path 12 as the circulation system element. An example in which liquid is circulated and driven in a container 19 having (for example, a cylindrical cross section) will be described. As a drive means, a stirring blade 20 is provided instead of the drive pump 13. The container measuring unit 21 has a function of selectively measuring the reaction capsule flowing in the container in consideration of a cyclic flow locus and acquiring a predetermined detection signal from the inside of the capsule. Except for the container 19, the stirring blade 20, and the container measuring unit 21, it is the same as FIG. 3. Here, the stirring blade 20 may be a magnetic stirring means using a magnetic chip that can be circulated and a non-contact stirring means using an ultrasonic flow.

  As an epoch-making application example by a circulatory system element, it can be used for in-vivo diagnosis as shown in FIG. 5 by selecting materials or the like so that immune reaction by a reaction capsule does not occur. That is, by injecting into the vein 103 using the syringe 102 the reaction capsule 101 of a quantity that can ignore the influence on living organisms (for example, the total transfusion amount of artificial blood cells as an artificial oxygen carrier) or less, (In FIG. 5, for the sake of convenience, the reaction capsule 11 is shown on the distal end side of the arm upstream of the vein, but in reality the shoulder side downstream of the injection site is shown. To be transported to). Here, the flow paths in FIG. 3 are veins and arteries, and the pump is the heart. At least in the circulatory system of the human body 105 as shown in FIG. 5, there is a possibility that several or more reaction capsules 101 can be measured per blood vessel by measuring for 1 minute by the pump function of the heart. The measurement time and measurement timing may be determined according to the inspection item, the lifestyle of the subject, and the like. For example, the measuring device may have a form as shown in FIG. The measuring device 201 includes an attachment unit 203 that can be easily attached to a part of the arm 202 (a wrist in FIG. 6), a measurement unit 204 that can selectively measure a signal from a reaction capsule among components that pass through the blood vessel, The measurement data is received from the measurement unit 204 via the display unit 205 for displaying the measurement data from the measurement unit and the transmission / reception medium means 206 such as an electric wire (which may be wireless), the analysis is performed, and the analysis result is further transmitted. And a determination mark display unit 208 that displays a determination mark based on the analysis result transmitted from the analysis unit 207. It is more preferable that the measuring device has a configuration that can be carried as required. Any means for noninvasive measurement from outside the body may be applied so that percutaneous detection can be performed. In mammals such as humans, cats, pigs, cows, horses, dogs and monkeys, it is preferable to perform measurement at sites where blood vessels such as arms, legs, fingers and eyes are easily accessible. The reaction capsule to be introduced into the body may be configured to have the same treatment and life support functions as conventional blood transfusion materials. During transfusion, an acceptable amount of reaction capsule can be introduced into the body at the same time. Since reaction capsules circulating in the body always exist under the average reaction conditions in the body, it is possible to provide means for monitoring routine fluctuations for a long period of time without taking blood samples each time. Since the reaction capsule circulates in the body for a long period of time like natural blood components, it is naturally discharged or decomposed outside the body, so that it is not necessary to take out the reaction capsule outside the body.

Eighth Embodiment FIG. 7 shows an example in which the reaction capsule is applied to a non-circulating system.
7 includes a non-circulation system 301 and a plurality of receiving units 302 (three in FIG. 7) that receive detection signals from the reaction capsule 11 moving through the passage of the non-circulation system 301 at a desired examination site. . Here, the capsules described in the first to second embodiments are used in the gastrointestinal tract as an in-vivo non-circulation system and the non-circulation type flow path (for example, a flow cytometer) as an external non-circulation system. it can. An injection means such as a syringe 303 is suitable for introducing the capsule into the non-circulating system 201. Especially when the reaction capsule is applied to a non-circulatory body flow path such as the gastrointestinal tract, it is continuously monitored for several days until defecation by a simple administration method such as mouth, nose, eye drops, etc. As a result, it is possible to provide a biochemical exploration capsule that is impossible with a capsule endoscope. In some cases, a capsule endoscope and a reaction capsule may be used together, or a dedicated launcher may be provided in the capsule endoscope or fiber endoscope so that the reaction capsule is directly administered into the body at a required site. . However, it is preferable to use a capsule fused with a solid material such as ceramic developed by DDS (drug delivery) technology so that the function can be stably maintained in the digestive juice secreted by the digestive organs.

Ninth Embodiment FIGS. 8 and 9 show an example in which a reaction capsule is used in a filter state.
In FIG. 8, a ring-shaped reaction capsule 31 having an open center is fixed to a porous plate-like support (for example, a substrate type or a sheet type) 32. Examples of the material for the plate-like support include glass, silicon, aluminum oxide, plastic, and rubber. In FIG. 9, the reaction capsule 11 is accommodated in a bowl-shaped channel 41 such as a chromatographic column. The bowl-shaped channel 41 accommodates a desired number of reaction capsules 11 in a flow-through bowl through a plurality of partition filters 42. Depending on the number of partition filters, various reaction capsules 11 can be accommodated in a plurality of baskets as shown in the figure. By providing the reaction capsule group configured in the filter shape as shown in FIGS. 8 and 9, a high-throughput flow-through type reaction apparatus can be provided while using the capsule described in the above-described embodiment.

Tenth Embodiment FIG. 10 shows an example in which a reaction capsule is formed in a film shape.
FIG. 10 shows an example in which the reaction capsule 11 is embedded in a multilayer film 51 having liquid permeability.
That is, the example of FIG. 10 constitutes a dry reaction apparatus, and includes a diffusion layer 54 for spreading a liquid 53 containing a target material spotted from a dispensing means 52 such as a pipetter, and a reaction capsule 11. A wet layer 55 that is embedded and can be wetted by the liquid 53 from the diffusion layer 54 and a water absorption layer 56 that absorbs excess water are provided. The diffusion layer 54 is preferably a porous member (for example, a gel or a small-diameter optical fiber bundle) excellent in diffusibility and light transmittance. According to the configuration shown in FIG. 10, even in the dry inspection system, it is possible to execute the reaction in the capsule described above and detect the reaction in the capsule.

Eleventh Embodiment FIG. 11 is a cross-sectional view showing a channel-shaped reaction capsule integrated with a flow-path type reaction container, which is a modification of the above-described reaction capsule. That is, FIG. 11 shows that a plurality of semi-transparent parts are respectively formed on the upstream surface and the partial region of the downstream surface of the multi-channel 62 having a large number of independent through-channels along the flow path direction. The film 63 is fixed at an opposing position. Here, the inner diameter and the cross-sectional shape of the flow path may vary. The space restricted by the pair of facing semipermeable membranes 63 and the multichannel 62 forms a capsule-like space equivalent to the reaction capsule formed by the semipermeable membrane 7 described in the third embodiment. By providing a plurality of pairs of semipermeable membranes 63 (five pairs in the figure) in the multichannel 62, a large number of closing reactions can be performed. The channel portion where the semipermeable membrane 63 is not disposed functions as a flow-through means for the liquid containing the target. Instead of the semipermeable membrane 63, various membranes with receptors as described in the first or second embodiment may be used.

Twelfth Embodiment FIG. 12 is a sectional view showing another example of the eleventh embodiment and showing a cylindrical reaction capsule integrated with a recessed container. That is, in FIG. 12, a plurality of flat bottom wells 72 are formed on the bottom surface of the well 71, and the upper open end of each well 71 is formed with various artificial membranes (liposomes and liposomes as described in the second embodiment or the third embodiment). (Semi-permeable membrane) 73. Here, the inner diameter, cross-sectional shape, number, and arrangement density of the wells may vary. In the case of this example as well, a large number of closing reactions can be performed as in the case of the flow channel integrated reaction capsule of FIG.

Thirteenth Embodiment FIG. 13 is a cross-sectional view showing an improved reaction capsule that can be commonly applied to all the reaction capsules described above. That is, the reaction capsule 401 in FIG. 13 accommodates therein a sustained release capsule 403 that gradually releases a raw material component 402 as a raw material necessary for the synthesis reaction of the reaction component 5. The raw material component 402 is an amount necessary for the monitoring of the reaction capsule 401 over a long period of time, is stored in the sustained release capsule 403 at a high density, and is gradually released into the detection reaction unit 3 by a certain amount with time. It has become. The structure of the sustained-release capsule can be referred to the drug sustained-release capsule used in the DDS technology. Preferably, a configuration that can always be adjusted to a constant concentration by osmotic pressure or the like according to the consumption amount of the raw material component 402 may be employed.

Fourteenth Embodiment FIG. 14 is a cross-sectional view showing another improved reaction capsule that can be commonly applied to all the reaction capsules described above. That is, the reaction capsule 501 of FIG. 14 has a plurality of measurement regions 502, 503, 504, 505, and 506 for measuring the detection reaction inside the capsule. Here, the measurement region is a region that can be used when a plurality of types of reactions are executed in the detection reaction unit or when a large number of reaction capsules 501 are individually identified. The plurality of measurement regions 502, 503, 504, 505, and 506 are arranged evenly around the reaction capsule 501, thereby further ensuring the acquisition of measurement data by the measurement device. The measuring device can obtain various kinds of reaction results and manage the ID of the reaction capsule 501 based on the measurement data obtained in each measurement region 502, 503, 504, 505, 506.

  As mentioned above, although embodiment of this invention was described, the following changes function effectively for this invention. For example, although the embodiment described above has been described for use in medical treatment, particularly for diagnostic purposes, it may be used for other purposes. As for the receptor for recognizing the target substance, if a plurality of types of receptors corresponding to two or more types of target substances are provided in the same or different reaction capsules, a multi-item reaction can be executed efficiently. In the second embodiment, an example using liposome particles that are artificial capsules using a lipid membrane as an outer shell has been described. However, a cell membrane having a similar lipid structure may be used as an outer shell of a capsule. Furthermore, the reaction container or the reaction support when the reaction capsule is used outside the body can be applied in various forms. For example, an impermeable two-dimensional substrate such as a silicon wafer or glass, various beads or fine particles, various porous substrates, various gels, various capillary type arrays (Japanese Patent Laid-Open Nos. 11-75812 and 2001-153860). Can be applied). In particular, when a non-permeable two-dimensional substrate is used, it can be formed into a flat hollow cell surrounding the entire substrate. In flat hollow cells, branching passages with partially different widths are provided, and the flow conditions are changed for each branching passage by appropriately adding a mechanism for electrochemically or mechanically controlling the flow direction of the fluid. It becomes possible to do. In addition, since various beads, various porous substrates, and various gels have a large surface area, the efficiency is improved by performing various processes while flowing the solution, but the efficiency is further improved by applying the present invention. This is preferable. When various beads or fine particles flow together in a fluid, it is preferable to set the flow rate so that particle sedimentation does not always occur. In addition, the capillary type array is configured to connect a sample or reagent as a fluid to a pressure supply device such as a pump with respect to a reagent solidified on the inner wall surface of the capillary or a plurality of fine particle surfaces introduced into the capillary. This is preferable because it can be an automated device that enables reciprocal transfer. If the measurement is performed a plurality of times during the reciprocating transfer of the fluid, there is also an advantage that the reaction result can be obtained efficiently from all the portions in the fluid.

FIG. 1 is a sectional view showing a reaction state by a reaction capsule in which reactivity is imparted to erythrocytes. FIG. 2 is a cross-sectional view showing a configuration of a reaction capsule in which reactivity is imparted to the semipermeable membrane 7. FIG. 3 is a diagram illustrating a configuration of an inspection system in which a reaction capsule is applied to a circulation channel. FIG. 4 is a diagram showing a configuration of an inspection system in which a reaction capsule is applied to a circulation system container. FIG. 5 is a schematic diagram showing a method of a circulatory system test that enables in-vivo diagnosis. FIG. 6 is a diagram showing the configuration of an inspection device and system applied to the inspection of FIG. FIG. 7 is a diagram showing an example in which the reaction capsule is applied to a non-circulating system. FIG. 8 is a diagram illustrating an example in which the reaction capsule is used in a filter state. FIG. 9 is a diagram showing another example of using the reaction capsule in a filter state. FIG. 10 is a diagram showing an example in which the reaction capsule is configured in a film shape. FIG. 11 is a diagram showing an example in which the reaction capsule is integrated with the flow channel container. FIG. 12 is a diagram showing an example in which the reaction capsule is integrated with the hollow container. FIG. 13 is a cross-sectional view showing an improved reaction capsule containing a sustained-release capsule. FIG. 14 is a view showing an improved reaction capsule having a plurality of measurement regions.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Target recognition part 2 ... Receptor 3 ... Detection reaction part 4 ... Transmitting substance 5 ... Reaction component 6 ... Target substance 7 ... Semipermeable membrane 11, 101, 31, 301, 401 ... Reaction capsule 12 ... Channel 13 ... Pump drive Unit 14 ... flow channel measurement unit 15 ... analysis unit 16 ... display unit 18 ... liquid 19 ... container 20 ... stirring blade 21 ... container measurement unit

Claims (5)

A reaction capsule for performing a reaction in a liquid, comprising a receptor for recognizing a target substance and a detection reaction unit for executing a reaction necessary for detection based on recognition information from the receptor. A reaction using a reaction capsule, comprising: a step of bringing a reaction capsule into contact with a liquid containing a target substance; and a step of starting another reaction in the capsule in conjunction with recognition of a receptor provided in the reaction capsule. Method. An inspection system using a reaction capsule, comprising: a measurement unit that selectively measures a reaction result in the reaction capsule in a liquid environment including the reaction capsule. The inspection system using the reaction capsule according to claim 3, further comprising a display unit for displaying data of the measurement unit. The inspection system using the reaction capsule according to claim 3 or 4, further comprising: an analysis unit that analyzes data of the measurement unit; and a determination display unit that displays a determination result of the analysis unit.

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