JP2006129713A - Laboratory dish filled with medium and method for producing and controlling the same - Google Patents

Laboratory dish filled with medium and method for producing and controlling the same Download PDF

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JP2006129713A
JP2006129713A JP2004319070A JP2004319070A JP2006129713A JP 2006129713 A JP2006129713 A JP 2006129713A JP 2004319070 A JP2004319070 A JP 2004319070A JP 2004319070 A JP2004319070 A JP 2004319070A JP 2006129713 A JP2006129713 A JP 2006129713A
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medium
petri dish
irradiation
management information
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Shinji Watanabe
晋次 渡辺
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Morinaga Milk Industry Co Ltd
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Morinaga Milk Industry Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish

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Abstract

<P>PROBLEM TO BE SOLVED: To provide a laboratory dish filled with a medium, capable of being produced and controlled by printing production/control information at the bottom surface of the dish body without obstructing the observation of colonies, and a method for producing/controlling the same. <P>SOLUTION: The method for producing/controlling the laboratory dish filled with the medium is characterized by printing the production/control information with an ultraviolet light fluorescent ink capable of being observable by an eyesight under the irradiation of ultraviolet light but not observable by the eyesight under the irradiation of visible light at the bottom surface of the laboratory dish body of the laboratory dish filled with the medium and producing/controlling it by identifying the production/control information under the irradiation of the ultraviolet light. The laboratory dish is characterized by being printed with the production/control information with the ultraviolet light fluorescent ink capable of being observable by the eyesight under the irradiation of the ultraviolet light but not observable by the eyesight under the irradiation of the visible light. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

本発明は、培地入りシャーレ（通称「生培地」）の製造管理、品種種別、品質管理に有効な培地入りシャーレの製造管理方法及び培地入りシャーレに関する。   The present invention relates to a production management method for a petri dish containing a medium effective for manufacturing management, variety classification, and quality control of a petri dish containing a medium (commonly referred to as “raw medium”) and a petri dish containing a medium.

生培地は、一般に５〜１０枚が包装単位として袋に詰められており、品種、ロット、有効期限等の製造管理情報の表示は、袋に貼られたラベルに記載されていた。このため、袋から出した生培地の品種は色でのみ区別されていた。
特開平６−２２５７５３号公報 特開平６−１７４７３２号公報 特許第２６３１９５２号公報 In general, 5 to 10 raw media are packed in a bag as a packaging unit, and the display of production management information such as the type, lot, and expiration date is described on a label attached to the bag. For this reason, the cultivars of the raw medium taken out of the bag were distinguished only by color.
JP-A-6-225753 JP-A-6-174732 Japanese Patent No. 2631952

しかし、生培地の色は、赤（デソオキシコーレイト培地、Ｏ−１５７用、ＤＨＬ寒天）、又は黄色（標準寒天培地、ＳＣＤ寒天培地、ガンジタＧＳ培地）が多く、色のみでは識別は困難であった。   However, the color of the raw medium is red (desoxycholate medium, for O-157, DHL agar) or yellow (standard agar medium, SCD agar medium, Gandita GS medium), and it is difficult to distinguish by color alone. there were.

また、シャーレの蓋に黒色のインクで製造管理情報を印字した商品も一部あるが、蓋を外すと前述と同じ理由で識別が困難になる。   Some products have manufacturing management information printed with black ink on a petri dish lid. However, if the lid is removed, identification becomes difficult for the same reason as described above.

また、シャーレ本体底部に印字すると、培養後のコロニー確認時にインクが邪魔になり見づらいという問題があった。図２は、可視光照射下で目視できるインクを用いてシャーレ本体２の底面に製造管理情報４を印字した培地入りシャーレ１を例示する底面図である。図２に示すように、可視光照射下で目視できるインクを用いてシャーレ本体２の底面に印字すると、培養後のコロニー確認時、製造管理情報４とコロニー３が重なって見にくくなってしまう。
さらに、２分割シャーレでは使用できなかった。 Further, when printing is performed on the bottom of the petri dish body, there is a problem that it is difficult to see the ink because it becomes an obstacle when confirming colonies after culturing. FIG. 2 is a bottom view illustrating the medium-containing petri dish 1 in which the production management information 4 is printed on the bottom surface of the petri dish body 2 using ink that can be seen under visible light irradiation. As shown in FIG. 2, if printing is performed on the bottom surface of the petri dish body 2 using ink that can be visually observed under irradiation with visible light, the production management information 4 and the colonies 3 overlap each other when the colonies are confirmed after culturing.
Furthermore, it could not be used in a two-part petri dish.

本発明は前記事情に鑑みてなされ、コロニー観察の邪魔になることなくシャーレ本体底面に製造管理情報を印字して製造管理可能な培地入りシャーレとその製造管理方法の提供を目的とする。   The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a medium-containing petri dish that can be manufactured and managed by printing manufacturing management information on the bottom of the petri dish body without interfering with colony observation, and a manufacturing management method thereof.

前記目的を達成するため、本発明は、培地入りシャーレのシャーレ本体底面に、紫外線照射下で目視可能であり、可視光照射下では目視不可能な紫外線蛍光インクによって製造管理情報を印字し、紫外線照射下で製造管理情報を識別して製造管理することを特徴とする培地入りシャーレの製造管理方法を提供する。   In order to achieve the above object, the present invention prints production management information on a bottom surface of a petri dish containing a culture medium with ultraviolet fluorescent ink that is visible under ultraviolet irradiation and is not visible under visible light irradiation. Provided is a production management method for a petri dish containing a culture medium characterized by identifying and managing production management information under irradiation.

また本発明は、培地入りシャーレのシャーレ本体底面に、紫外線照射下で目視可能であり、可視光照射下では目視不可能な紫外線蛍光インクによって製造管理情報が印字されていることを特徴とする培地入りシャーレを提供する。   Further, the present invention provides a culture medium characterized in that production management information is printed on the bottom surface of a petri dish containing a culture medium with ultraviolet fluorescent ink that is visible under ultraviolet irradiation and is not visible under visible light irradiation. Provide a petri dish.

本発明によれば、生培地の品種、ロット、有効期限、通し番号等の製造管理情報が、コロニー観察の邪魔になることなく印字でき、確実に製造管理することができる。   According to the present invention, production management information such as the type, lot, expiration date, serial number, etc., of a live medium can be printed without disturbing colony observation, and production management can be performed reliably.

以下、図面を参照して本発明の実施形態を説明する。
図１は、本発明に係る培地入りシャーレの製造管理方法の第１実施形態を示す図であり、図１中、符号１０は培地入りシャーレ、１１はシャーレ本体、１２は蓋、１３は培地、１４はコンベア、１５はインクジェットプリンタ、１６は紫外線蛍光インク（以下、ＵＶ蛍光インクと記す。）、１７は製造管理情報である。 Hereinafter, embodiments of the present invention will be described with reference to the drawings.
FIG. 1 is a diagram showing a first embodiment of a method for manufacturing and managing a petri dish with a medium according to the present invention. In FIG. 1, reference numeral 10 denotes a petri dish containing a medium, 11 denotes a petri dish body, 12 denotes a lid, 13 denotes a medium, Reference numeral 14 denotes a conveyor, 15 denotes an inkjet printer, 16 denotes ultraviolet fluorescent ink (hereinafter referred to as UV fluorescent ink), and 17 denotes manufacturing management information.

本発明の培地入りシャーレの製造管理方法は、培地入りシャーレ１０のシャーレ本体１１底面に、紫外線照射下で目視可能であり、可視光照射下では目視不可能なＵＶ蛍光インク１６によって製造管理情報１７を印字し、紫外線照射下で製造管理情報を識別して製造管理することを特徴としている。   The manufacturing management method of the petri dish with culture medium of the present invention can be visually observed on the bottom surface of the petri dish main body 11 of the petri dish 10 with culture medium under UV irradiation, but with UV fluorescent ink 16 that is not visible under visible light irradiation. Is printed, and manufacturing management information is identified and managed under ultraviolet irradiation.

本発明の製造管理方法において用いる培地入りシャーレ１０は、皿状のシャーレ本体１１と、その開口を塞ぐ蓋１２と、シャーレ本体１１内に収容した培地１３とからなり、そのシャーレ本体１１の底面に、紫外線照射下で目視可能であり、可視光照射下では目視不可能なＵＶ蛍光インク１６によって製造管理情報１７が印字されていることを特徴としている。   A petri dish 10 with a medium used in the production management method of the present invention comprises a dish-shaped petri dish body 11, a lid 12 that closes the opening thereof, and a culture medium 13 accommodated in the petri dish body 11. The production management information 17 is printed by the UV fluorescent ink 16 that is visible under ultraviolet irradiation and is not visible under visible light irradiation.

この製造管理情報１７としては、培地品種、製造年月日、通し番号、製造ロット、有効期限、製造工場記号などが挙げられ、これらの１種以上を数字又は記号によりＵＶ蛍光インク１６で印字してある。   The production management information 17 includes medium type, production date, serial number, production lot, expiration date, production factory symbol, etc., and one or more of these are printed with the UV fluorescent ink 16 by numbers or symbols. is there.

この培地入りシャーレ１０に収容される培地としては特に限定されず、例えば、デソオキシコーレイト培地、Ｏ−１５７用、ＤＨＬ寒天、標準寒天培地、ＳＣＤ寒天培地、ガンジタＧＳ培地などが挙げられる。   The medium accommodated in the petri dish 10 containing the medium is not particularly limited, and examples thereof include desoxycholate medium, for O-157, DHL agar, standard agar medium, SCD agar medium, and Gandita GS medium.

図１は、培地入りシャーレ１０に製造管理情報１７を印字する方法の具体例を示す。この印字方法では、印字位置となる部分の中央を切り欠いたコンベア１４と、この印字位置の直下に配置されたインクジェットプリンタ１５を備えた装置を用い、コンベア１４上を所定方向に搬送される培地入りシャーレ１０が前記印字位置に達すると、インクジェットプリンタ１５が駆動し、シャーレ本体１１の底面に向けてＵＶ蛍光インク１６を噴射し、所望の製造管理情報１７が印字される。   FIG. 1 shows a specific example of a method for printing the production management information 17 on the petri dish 10 with medium. In this printing method, a culture medium transported in a predetermined direction on the conveyor 14 using an apparatus including a conveyor 14 in which a center of a printing position is cut out and an ink jet printer 15 disposed immediately below the printing position. When the incoming petri dish 10 reaches the printing position, the ink jet printer 15 is driven, the UV fluorescent ink 16 is ejected toward the bottom surface of the petri dish body 11, and desired production management information 17 is printed.

この培地入りシャーレ１０に印字された製造管理情報１７は、太陽光や蛍光灯などの可視光照射下では目視不可能であり、紫外光照射下で目視可能となる。このようにＵＶ蛍光インクで印字された製造管理情報１７を目視するためには、紫外線ランプが必要になるが、一般に蛍光反応を利用した検査の確認用に使用されている紫外線ランプがそのまま利用できる。   The production management information 17 printed on the petri dish 10 with medium is not visible under visible light irradiation such as sunlight or a fluorescent lamp, and is visible under ultraviolet light irradiation. In order to visually check the production management information 17 printed with the UV fluorescent ink in this manner, an ultraviolet lamp is required. However, an ultraviolet lamp that is generally used for checking an inspection using a fluorescent reaction can be used as it is. .

この培地入りシャーレ１０は、必要に応じて紫外線を照射して製造管理情報１７を目視することで、培地品種、製造年月日、通し番号、製造ロット、有効期限、製造工場記号などの情報を確認して製造管理に役立てることができる。この製造管理情報１７は、可視光照射下では目視不可能であるので、コロニー観察時にこの製造管理情報１７が邪魔になることが無く、コロニー１８を正確に計数できる。   This petri dish 10 with medium is irradiated with ultraviolet rays as necessary, and the manufacturing management information 17 is visually checked to confirm information such as the medium type, manufacturing date, serial number, manufacturing lot, expiration date, manufacturing factory symbol, etc. And can be used for manufacturing management. Since the manufacturing management information 17 is not visible under irradiation with visible light, the manufacturing management information 17 does not get in the way during colony observation, and the colonies 18 can be accurately counted.

図３は、この培地入りシャーレ１０を用いて培養後、出現したコロニーを観察するために、太陽光又は蛍光灯下でシャーレ本体１１を底面側から観察した状態を示している。図３に示すように、太陽光又は蛍光灯下では、シャーレ本体１１の裏面に印字した製造管理情報１７は目視されず、製造管理情報１７が邪魔になること無くコロニー１８を正確に計数できる。   FIG. 3 shows a state in which the petri dish body 11 is observed from the bottom side under sunlight or a fluorescent lamp in order to observe colonies that appear after culturing using the petri dish 10 with medium. As shown in FIG. 3, under sunlight or a fluorescent lamp, the production management information 17 printed on the back surface of the petri dish body 11 is not visually observed, and the colonies 18 can be accurately counted without the production management information 17 getting in the way.

図４は、図３と同じ培養後の培地入りシャーレ１０の底面に紫外線ランプからの紫外線を照射した状態を示している。図４に示すように、紫外線照射下では、ＵＶ蛍光インクにより印字した製造管理情報１７が明確に目視できる。   FIG. 4 shows a state in which ultraviolet rays from an ultraviolet lamp are irradiated on the bottom surface of the culture medium-containing petri dish 10 after the same culture as in FIG. As shown in FIG. 4, under ultraviolet irradiation, the production management information 17 printed with UV fluorescent ink can be clearly seen.

図５は、本発明に係る培地入りシャーレの製造管理方法の第２実施形態を示す図であり、本実施形態では二分割シャーレ１９の一方の分割部分２０と他方の分割部分２１のそれぞれの底面に製造管理情報２２，２３をＵＶ蛍光インクにて印字したことを特徴としている。   FIG. 5 is a diagram showing a second embodiment of the method for manufacturing and managing a petri dish with medium according to the present invention. In this embodiment, the bottom surfaces of one divided portion 20 and the other divided portion 21 of the two-divided petri dish 19 are shown. The manufacturing management information 22 and 23 are printed with UV fluorescent ink.

本実施形態の二分割シャーレ１９の場合も、前記第１実施形態の培地入りシャーレの製造管理方法と同じ効果を得ることができる。   Also in the case of the two-divided petri dish 19 of the present embodiment, the same effect as the production management method for the medium-containing petri dish of the first embodiment can be obtained.

本発明の製造管理方法の一実施形態を示す構成図である。It is a block diagram which shows one Embodiment of the manufacturing management method of this invention. 通常インクで製造管理情報を印字した培地入りシャーレのコロニー観察状態を示す底面図である。It is a bottom view which shows the colony observation state of the petri dish with a culture medium which printed manufacture management information with normal ink. 本発明の培地入りシャーレによる可視光照射下のコロニー観察状態を示す底面図である。It is a bottom view which shows the colony observation state under visible light irradiation by the petri dish containing the culture medium of this invention. 本発明の培地入りシャーレによる紫外線照射下のコロニー観察状態を示す底面図である。It is a bottom view which shows the colony observation state under the ultraviolet irradiation by the petri dish containing the culture medium of this invention. 本発明に係る二分割タイプの培地入りシャーレの底面図である。It is a bottom view of the petri dish with a culture medium of the 2 division type concerning the present invention.

符号の説明Explanation of symbols

１０…培地入りシャーレ、１１…シャーレ本体、１２…蓋、１３…培地、１４…コンベア、１５…インクジェットプリンタ、１６…ＵＶ蛍光インク（紫外線蛍光インク）、１７…製造管理情報、１８…コロニー、１９…二分割シャーレ、２０…一方の分割部分、２１…他方の分割部分、２２，２３…製造管理情報。
DESCRIPTION OF SYMBOLS 10 ... Petri dish with culture medium, 11 ... Petri dish main body, 12 ... Cover, 13 ... Medium, 14 ... Conveyor, 15 ... Inkjet printer, 16 ... UV fluorescent ink (ultraviolet fluorescent ink), 17 ... Manufacturing management information, 18 ... Colony, 19 ... two-divided petri dishes, 20 ... one divided part, 21 ... the other divided part, 22, 23 ... manufacturing management information.

Claims (2)

培地入りシャーレのシャーレ本体底面に、紫外線照射下で目視可能であり、可視光照射下では目視不可能な紫外線蛍光インクによって製造管理情報を印字し、紫外線照射下で製造管理情報を識別して製造管理することを特徴とする培地入りシャーレの製造管理方法。   Manufacturing management information is printed on the bottom of the petri dish containing medium with UV fluorescent ink that is visible under UV irradiation but not visible under visible light irradiation, and manufacturing management information is identified under UV irradiation. A method for producing and managing a petri dish containing a culture medium, characterized by comprising: 培地入りシャーレのシャーレ本体底面に、紫外線照射下で目視可能であり、可視光照射下では目視不可能な紫外線蛍光インクによって製造管理情報が印字されていることを特徴とする培地入りシャーレ。
A petri dish with medium, wherein the production control information is printed on the bottom surface of the petri dish of the petri dish containing the medium with ultraviolet fluorescent ink that is visible under ultraviolet irradiation and is not visible under visible light irradiation.
JP2004319070A 2004-11-02 2004-11-02 Laboratory dish filled with medium and method for producing and controlling the same Withdrawn JP2006129713A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015037407A1 (en) * 2013-09-11 2015-03-19 大日本印刷株式会社 Cell culture vessel
JP2015053876A (en) * 2013-09-11 2015-03-23 大日本印刷株式会社 Culture vessel
JP2016106635A (en) * 2013-09-11 2016-06-20 大日本印刷株式会社 Cell culture container
US11680236B2 (en) 2017-11-30 2023-06-20 Corning Incorporated Thin, uniform, stackable petri dish

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015037407A1 (en) * 2013-09-11 2015-03-19 大日本印刷株式会社 Cell culture vessel
JP2015053876A (en) * 2013-09-11 2015-03-23 大日本印刷株式会社 Culture vessel
JP2015077121A (en) * 2013-09-11 2015-04-23 大日本印刷株式会社 Cell culture container
JP2016106635A (en) * 2013-09-11 2016-06-20 大日本印刷株式会社 Cell culture container
US10557111B2 (en) 2013-09-11 2020-02-11 Dai Nippon Printing Co., Ltd. Cell culture vessel
US11680236B2 (en) 2017-11-30 2023-06-20 Corning Incorporated Thin, uniform, stackable petri dish

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