JP2006047042A - Implement used in analyzing method using capillary array sheet - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a capillary array sheet holder housing container unnecessary to attach and detach several screws at each time when hybridization treatment is performed and capable of reducing the load of a worker even if the number of treatments is increased. <P>SOLUTION: In the housing container for housing a capillary array sheet holder for housing the capillary array sheet holder constituted so that a capture probe is fixed to a high-molecular gel, at least one capillary array sheet containing a plurality of through-holes which hold the gel can be housed and an opening capable of exposing the high-molecular gel of the sheet is provided, the housing container is divided into a container and a lid and a rivet is used for fixing the container and the lid. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a tool for preventing damage to a capillary array sheet in reaction processing such as hybridization and improving handling of a microarray when performing gene expression analysis using a DNA microarray or the like.

  There is an analysis method called a DNA microarray method (DNA chip method) as a method for performing batch expression analysis of a large number of genes. In this method, a large number of DNA fragments are aligned and immobilized on a flat substrate piece called a microarray, and nucleic acid detection based on a nucleic acid: nucleic acid hybridization reaction is performed on each immobilized DNA fragment. And a method for quantitative determination.

As a method of using the microarray method, for example, a sample solution containing a sample in which a gene expressed by a researcher's target cell is labeled with a fluorescent dye is applied to the microarray, hybridization is performed, and complementary nucleic acids are bound to each other. There is a method of detecting and quantifying nucleic acid by reading a section where a hybrid is formed at high speed with a high resolution analyzer.

In addition to the above-described flat substrate piece, for example, a capillary array sheet in which a capture probe is fixed to a polymer gel and a plurality of through-hole portions holding the gel is included is known. The manufacturing method is disclosed in Document 1, for example.

In Patent Document 1, a plurality of hollow fibers are prepared, the hollow fibers are bundled, a hollow fiber array is formed by fixing the bundle with a resin, and a capture probe is attached from one end of the array. After introducing a polymerizable monomer solution such as acrylamide into the hollow part of each hollow fiber and polymerizing in the hollow part, the resin immobilization part of the hollow fiber array is repeatedly cut in the direction intersecting the longitudinal direction of the hollow fiber. Discloses a method for producing a capillary array sheet.

The capillary array sheet exposes the polymer gel with the capture probe immobilized on the sheet surface, and it is necessary to handle it with great care so as not to damage the delicate gel part exposed on the surface in the reaction process. Therefore, the capillary array sheet must be protected with a suitable container.

The container used for the hybridization treatment is generally a storage container used for hybridization of a slide glass type microarray. The slide glass is inserted into the storage container, and a lid is applied from above. What is fixed with a screw of a book is known.

In such a storage container, it is necessary to attach and detach several screws every time the hybridization process is performed, and there is a demerit that an increase in the number of processes increases the burden on the operator.
JP 2001-133453 JP

  The object of the present invention is to prevent damage to the capillary array sheet in a reaction process when an analysis is performed using a capillary array sheet in which a capture probe is fixed to a polymer gel and includes a plurality of through-hole portions holding the gel. It is to provide a tool for preventing and improving the handling of capillary array sheets in their processing.

  As a result of intensive investigations in view of the above problems, the present inventors have found that the above problem can be solved by using a storage container that does not use screws when fixing the container part and the lid part of the storage container that stores the capillary array sheet holder. I found that the problem can be solved.

  That is, in the present invention, the capture probe is fixed to the polymer gel, and one or more capillary array sheets including a plurality of through-hole portions holding the gel can be stored, and the polymer gel of the sheet can be exposed. A storage container for storing a capillary array sheet holder having a flexible opening, wherein the storage container is divided into a container part and a lid part, and a rivet is used to fix the container part to fix the container part and the lid part. A feature of this is a cabinet array sheet holder storage container.

  Further, in the present invention, the capture probe is fixed to the polymer gel, one or more capillary array sheets including a plurality of through-hole portions holding the gel can be stored, and the polymer gel of the sheet can be exposed. A storage container for storing a capillary array sheet holder having a flexible opening, the storage container being divided into a container part and a lid part, and having a key-like claw structure on either the container part or the lid part The capillary array sheet holder storage container has a structure in which the claw meshes with the other.

  The storage container preferably further has an inlet through which liquid can be injected into the opening. Moreover, it is preferable to have a window that can be observed from the outside in the opening.

  By using the capillary array sheet holder storage container of the present invention, there is no risk of accidentally damaging the delicate gel filling part when operating the capillary array sheet, the handling is improved, and it is not a skilled worker. In addition, it is possible to carry out batch expression analysis of a large number of genes using a capillary array sheet in a simple and continuous manner.

  Hereinafter, the present invention will be described in detail with reference to the drawings.

FIG. 1 is a perspective view showing a capillary array sheet (1) (hereinafter simply referred to as an array) and a capillary array sheet holder (2) (hereinafter simply referred to as a holder) in which the array is stored. .

The array is composed of a sheet-like object having a plurality of through holes. A gel is held inside each through hole, and a capture probe is fixed to the gel. An example of a detailed manufacturing method is described in JP-A-2001-133453.

Storage of the array is carried out by inserting the array (1) into the holder body (2a) and fitting the holder lid (2b) into the holder body. In the housed state, the exposed gel portion of the array is exposed from the opening (2c) on the front and back sides, and the through-flow passage (2d) connecting the opening on the front and back surfaces forms grooves at two opposite positions across the array. It has a structure.

The material constituting the holder is not particularly limited as long as it does not contain a substance that inhibits a detection reaction such as a hybridization reaction or an antigen-antibody reaction. For example, a thermoplastic resin material such as polypropylene, polyethylene, polymethyl methacrylate, or polycarbonate can be used and mass-produced at low cost by injection molding or the like.

Here, in the case of using fluorescence detection at the time of detection, if the autofluorescence of the array holder is strong, the S / N ratio is lowered and the detection accuracy is lowered. Therefore, it is necessary to select a material with small autofluorescence.

Even if the material has a high autofluorescence, the addition of an additive that absorbs fluorescence, such as carbon black, can greatly reduce the autofluorescence, so various materials to which such an additive is added can also be used.

FIG. 2 is a schematic view showing the holder storage container. The holder storage container (10) includes a container part (11) and a lid part (12). The container part has a holder storage groove (11a) for storing the holder, a packing groove (11b) for inserting the packing, There is a rivet hole (11c) for inserting a resin rivet. The lid portion has an injection port (12a) that passes through the front and back for injecting the specimen liquid, a packing groove (12b) for storing the packing, and a rivet through hole (12c) through which the resin rivet passes. .

The inlet (12a) has a structure in which a screw-type lid (not shown) can be inserted.

FIG. 3 is a longitudinal sectional view of the holder storage container (10) shown in FIG. By pushing the resin rivets (15a) and (15b) through the rivet through hole (12c) and the rivet hole (11c), the upper and lower surfaces of the array come into contact with the lid and the packing (14) of the container, and the periphery of the array A space for sealing the specimen solution is formed in the opening.

As the resin rivet, for example, a product name “push rivet” manufactured by Koyo Fastener Co., Ltd., the pin body (15a) is pushed into the rivet body (15b), thereby dividing the vertical rivet provided at the lower part of the rivet body. The container part (11) and the cover part (12) can be tightly fixed by using a groove that is pushed outward and generates a caulking effect.

When removing the array in the container after completion of the reaction process such as hybridization, the caulking effect is canceled by pushing up the pin body (15a) of the resin rivet from the bottom side of the container part (11), and the resin rivet is removed. After that, it is possible to remove the lid (12) and take out the array in the container.

In this figure, the structure is fixed with four resin rivets. However, if the storage container can be sealed, two containers may be used. When storing a plurality of arrays in one storage container, the storage container The quantity of resin rivets may be increased in accordance with the size of.

FIG. 4 is a schematic view showing another form of the holder storage container.

The holder storage container (20) includes a main body (21) and a lid portion (22), and at least four claws (22b) are provided on the outer periphery of the lid portion (22). The main body (21) is also provided with a groove (21a) so as to engage with each other. The other structure is the same as that of the holder storage container (10).

The inlet (22a) has a structure in which a screw-type lid (not shown) can be inserted.

FIG. 5 is a cross-sectional view of the holder storage container (20) shown in FIG.

When the groove (21a) of the main body (21) and the claw (22b) of the lid portion (22) are engaged with each other, the packing (31) inserted into the packing groove provided in the main body and the lid portion is connected to the upper and lower surfaces of the array. A space for adhering to the specimen solution is formed in the opening.

When the array in the container is taken out after completion of the reaction process such as hybridization, the cover part can be removed by folding the claw (22b) of the cover part (22) outward, and the array in the container can be removed. You can take it out.

In this figure, the claws are fixed at four locations. However, if the storage container can be sealed, it may be provided at two locations. When storing a plurality of arrays in one storage container, You can increase the number of nails according to the size.

It is a perspective view which shows a capillary array sheet | seat and the capillary array sheet | seat holder of the state in which the array was accommodated. It is a schematic diagram which shows a capillary array sheet holder storage container. It is sectional drawing of the state which accommodated the capillary array sheet holder which accommodated the capillary array sheet in the capillary array sheet holder storage container. It is a schematic diagram which shows another form of a capillary array sheet | seat holder storage container. It is sectional drawing of the state which accommodated the capillary array sheet holder which accommodated the capillary array sheet in the capillary array sheet holder storage container.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 .... Capillary array sheet 1a ... Gel holding part 2 .... Capillary array sheet holder 2a ... Holder body 2b ... Holder lid 2c ... ··· Opening portion 2d ··· Through channel 10 ··· Holder holding container 11 ··· Container portion 11a ··· Holder holding groove 11b ··· Packing groove 11c ··· Rivet hole 12 ... Lid 12a ... Injection port 12b ... Packing groove 12c ... Rivet through hole 13 ... Window lid 14 ... Packing 15a ... ··· Pin body 15b ··· Rivet main body 20 ··· Holder holding container 21 ··· Main body portion 21a ··· Groove portion 22 ··· Lid portion 22a ··· Inlet 22b ····claw

Claims (4)

  Capillary having a capture probe fixed to a polymer gel, capable of storing one or more capillary array sheets including a plurality of through-hole portions holding the gel, and having an opening through which the polymer gel of the sheet can be exposed A storage container for storing an array sheet holder, wherein the storage container is divided into a container part and a lid part, and a rivet is used to fix the container part to fix the container part and the lid part. Array sheet holder storage container.   Capillary having a capture probe fixed to a polymer gel, capable of storing one or more capillary array sheets including a plurality of through-hole portions holding the gel, and having an opening through which the polymer gel of the sheet can be exposed A storage container for storing the array sheet holder, wherein the storage container is divided into a container part and a lid part, and either the container part or the lid part has a key-like claw structure, and the other part has the claw. A capillary array sheet holder storage container having a meshing structure.   The storage container according to claim 1, further comprising an inlet through which liquid can be injected into the opening. The container according to claim 3, further comprising a window that can be observed from the outside in the opening.

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