JP2006036749A - Active functional agent of ostrich blood - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an active functional agent given by preparing ostrich blood, without causing deactivation of fatigue recovery action, resistance to oxygen deficit, resistance to cold temperature, resistance to high temperature, an anticancer effect, and a vigor enhancing effect. <P>SOLUTION: This active functional agent comprises a freeze-dried powder of the ostrich blood or a polypeptide mixture which is formed by hydrolyzing serum and plasma of the ostrich blood with blended enzymes. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an ostrich blood active functional agent, more specifically freeze-dried powder of ostrich blood, polypeptide mixture formed by hydrolyzing ostrich blood serum and plasma with a specific enzyme at a specific pH and temperature, and combinations thereof It relates to the agent.

  Ostriches that inhabit China are roughly classified into three types: African ostriches, Western ostriches and Australian ostriches. Among them, African ostrich has a large body and a large amount of activity. On the other hand, the ostrich of Europe, the United States and Australia has a small body and a little mild temper, but the muscles of the thigh are slender and strong, and the speed reaches 70-90 km / h. It is known that there are special enzymes in the blood of these three ostriches, which can be taken up instantly and converted into a great deal of energy by muscle cells.

Since ostrich contains many useful ingredients, it has been used only as meat as in Patent Document 1, and there has been no precedent for using ostrich blood until now. When one mature ostrich was slaughtered, it was not used effectively, although about 10 liters of blood was produced. At mid-scale farms, 600-700 ostriches were slaughtered every year, and roughly 6500 liters of ostrich blood was discarded annually.
JP 2002-209555 A

  Therefore, the present inventor discovered for the first time that ostrich blood (abbreviation: OB) is resistant to fatigue, anoxia, low temperature, high temperature, sooyo (also referred to as auxiliary medicine), and anticancer effect. The ostrich blood freeze-dried powder (abbreviated as TOB dry powder or OB dry powder), and a polypeptide mixture (abbreviation: SPPDP) formed by hydrolyzing the same amount of serum and plasma with a combination enzyme at a specific pH and temperature, The present invention intends to provide a method for preparing ostrich blood and a compounding agent by inventing that each has fatigue recovery action, anti-oxygen deficiency, anti-high cold, anti-high temperature, anti-cancer effect and energy enhancing effect.

In order to solve the above-mentioned problem, the present invention according to claim 1 is a freeze-dried powder of ostrich blood (Total ostrich blood, abbreviated as TOB), which has fatigue recovery action, lack of anti-oxygen, anti-high cold, anti-high temperature, anti-resistance. It is an active functional agent for ostrich blood, characterized by its effects on cancer and energy enhancement.
The invention of claim 2 is a polypeptide dry powder (abbreviation: SPPDP) formed by hydrolyzing ostrich blood serum and plasma with a specific enzyme at a specific pH and temperature, and has a fatigue recovery action. It is an active functional agent for ostrich blood, which has anti-oxygen deficiency, anti-high cold, anti-high temperature, anti-cancer effect and energy enhancing effect.
These two substances are excellent in fatigue recovery action, anti-oxygen deficiency, anti-high cold, anti-high temperature, anti-cancer effect and energy enhancement effect, etc. Of course, even if both are mixed, fatigue recovery is achieved. It is excellent in action, anti-oxygen deficiency, anti-high cold, anti-high temperature, anti-cancer effect and energy enhancing effect.

The present invention according to claim 3 is characterized in that the compound enzyme that hydrolyzes the polypeptide mixture according to claim 2 includes at least one of papain, trypsin, and subtilisin, and the specific PH is between 6.8 and 7.5. The active functional agent for ostrich blood, characterized in that the specific temperature is 45-55 ° C. and the amount of the specific enzyme used is 0.05-1.0%. Thereby, the polypeptide mixture can be purified with high yield.
Here, the blending ratio of the blended enzyme that can purify the polypeptide mixture with the highest yield is papain: trypsin: subtilisin = 3: 1: 0.5. The manufacturing method at this time is a process described later in the examples. In addition, the polypeptide mixture can be purified with high yield even when the ratio of papain: trypsin = 3: 1.5 is used without using subtilisin. Furthermore, the polypeptide mixture can be purified with only one of the three types, papain, trypsin and subtilisin, although the yield is not high.

According to the present invention of claim 4, the ostrich blood freeze-dried powder of claim 1 and the polypeptide mixture of claim 2 all contain active protein and active polypeptide, and the active protein reaches 91.6%. It is an active functional agent for ostrich blood, characterized in that it contains 21 kinds of amino acids (Table 1 below), and the active polypeptide containing SPPDP is easily absorbed by human and animal bodies.
Here, for the 21 amino acids shown in Table 1 below, the content is also a value specific to ostrich blood, for example, a content that cannot be obtained by mixing a plurality of animal bloods. In addition, the content rate of the following Table 1 is a measured value, and this numerical value is not exact | strict, and there exists a range, such as an error, according to a classification, individual difference, physical condition, etc.

  The particularly preferred dosage is that the emergency function such as fatigue recovery, anti-oxygen deficiency, anti-high cold, anti-high temperature, etc. according to claim 1 is used in an amount of 100 to 240 mg / person / day by acute toxicity experiments and animal experiments. It is an active functional agent of ostrich blood characterized by being. Thereby, the best effect can be produced at the time of use.

  Further, the preferred dose is the use amount of the clinical anticancer action according to claim 1, which is 300 to 600 mg / person / day depending on the dose of the acute toxicity experiment and the animal experiment, It is an active functional agent for ostrich blood, characterized in that it is 260-300 mg / person / day depending on the dose of acute toxicity experiments and animal experiments. Thereby, the best effect can be produced at the time of use.

According to the present invention of claim 5, the ostrich blood freeze-dried powder according to claim 1 and / or the polypeptide mixture is mixed at any of the following blending ratios, and it is mainly useful that the ostrich blood freeze-dried powder and The ostrich blood combination, which is a mixture of the polypeptides and other combination preparations are useful in cooperation.
Formulation 1 (abbreviated as No. 1): TOB 400 mg, extract of coconut palm (Branchaceae Crassula) 200 mg, Western ginseng extract 200 mg, mulberry extract 200 mg.
Here, Western ginseng belongs to the family Araceae, and in addition to being native to North America, it has been successfully transplanted in Guangdong Province and is also called Guangdong ginseng, its taste is sweet and bitter, but the sex is cool and warm. It is different from ginseng. Looking at the effects of ginseng in modern medicine, it is known that blood pressure and cholesterol are lowered by the effects of diuretic fever, stool, sedation, and nutritional tonic by regulating body fluid metabolism.
Formulation 2 (abbreviation No. 2): 400 mg of TOB, 200 mg of extract of dried flower beans (kankatou), 200 mg of extract of western ginseng, 200 mg of extract of lees
Here, dried flower beans are a kind of wild dicotyledonous plant, and belong to the butterfly flower (Fordia Cauliflor Hemis). It has also been classified as a subfamily of Leguminosae. It grows naturally in the southwestern region of China, in the Taihu Mountain Range. Mass supply of raw materials depends on artificial cultivation. Dried flower beans are also known as Kisang, Ginseng, and Fushou Beans.
Formulation 3 (abbreviation No. 3): TOB 400mg, coconut (kokushi: sweet and flat taste, liver function protection, dried mature fruit of solanaceae) 200mg, Western ginseng Extract of 200mg, spiral algae powder 200mg.
Spirulina (Spirulina) is a natural algae peculiar to China that lives in freshwater lakes that have existed since 3.5 billion years ago, which is rich in calcium and magnesium, which contains polysaccharides rich in folic acid, chlorophyll, vitamins, and minerals. .
Formulation 4: 400 mg SPPDP, 200 mg coconut palm extract, 200 mg Western ginseng extract, 200 mg mulberry extract.
Formulation 5: 400 mg of SPPDP, 200 mg of dried flower bean extract, 200 mg of extract of Western ginseng, 200 mg of extract of nectar.
Formulation 6: SPPDP 400 mg, eggplant extract 200 mg, Western ginseng extract 200 mg, spiral algae powder 200 mg.
Formulation 7: TOB 200 mg, SPPDP 200 mg, coconut palm extract 200 mg, Western ginseng extract 200 mg, mulberry extract 200 mg.
Formulation 8: 200 mg TOB, 200 mg SPPDP, 200 mg dried flower bean extract, 200 mg extract from Western ginseng, 200 mg extract from nectar.
Formulation 9: 200 mg TOB, 200 mg SPPDP, 200 mg coconut extract, 200 mg western ginseng extract, 200 mg spiral algae powder.

The active function of ostrich blood, the method for preparing ostrich blood, and the formulation according to the present invention will be described in detail below.
1. Ostrich blood preparation:
1.1, Example 1: (Method for producing freeze-dried powder of ostrich blood)
Take 12 liters of fresh ostrich blood, place it in an ice dryer, lower the temperature to -40 ° C, quickly raise the temperature, and simultaneously depressurize: 0.095MPa, rise to 0 ° C, break the cell wall, The moisture is removed by steaming to obtain TOB (abbreviation: Total ostrich blood, TOB) which is 2.16Kg lyophilized powder. The yield is 18%. Grind with 60 mesh screen and pack into 1kg / bag. (See Figure 1)

1.2, Example 2: (Method for producing polypeptide mixture of ostrich blood)
Add sodium citrate anticoagulant to 10 liters (approx. 10 kg) of male ostrich blood, place in a centrifuge at 3000 rpm for 10 minutes, centrifuge for 10 minutes, serum (45%, approx. 4.5 kg) and plasma (55 %, About 5.5kg). Put serum and plasma in dialysis bags respectively and remove sodium citrate. One serum is lyophilized as in Example 1 (FIG. 1) to give 0.81 kg serum. The yield is 18.0%. The other 5.5 kg of plasma was slowly stirred and homogenized and placed in a hydrolysis can. Papain 27.5-55 g (corresponding to 0.05-1.0% of plasma) was added, the temperature was 50-55 ° C, and PH = 6.8-7.5. Keep warm for hours. Thereafter, the temperature is lowered to 45-55 ° C., 5.5 g of trypsin (corresponding to 0.1% of plasma) and 2.75 g of subtilisin (corresponding to 0.05% of plasma) are added, and the mixture is kept at 45-55 ° C. for 4 hours. Cover the hydrolysis can with the hydrolyzed can and circulate to 50-60 ° C, concentrate under reduced pressure (0.098Mpa) until the water content is ≤3%, grind it with a 100 mesh sieve, and dry the polypeptide 0.9625 kg of (polypeptide dry powder, abbreviation: PPDP) is obtained, and the yield is 17.5%. Package in 500g / bag.
When conducting animal tests, serum serum (serum) is mixed with polypeptide dry powder (PPDP) in an equal amount, and physiological saline is added to dissolve. Abbreviation: SPPDP. (See Figure 2)

1.3, Example 3: Formulation 1 (abbreviation No. 1): TOB is 400 mg, coconut palm extract is 200 mg, Western ginseng extract is 200 mg, and mulberry extract is 200 mg.
1.4, Example 4: Formulation 2 (abbreviated as No. 2): TOB is 400 mg, dried flower bean extract is 200 mg, Western ginseng extract is 200 mg, and lamb extract is 200 mg.
1.5, Example 5: Formulation 3 (abbreviation No. 3): TOB 400 mg, eggplant extract 200 mg, Western ginseng extract 200 mg, spiral algae powder 200 mg.

2. Experimental materials:
2.1, Raw materials: After pulverizing No.1, No.2, No.3 again, polish them enough in a mortar, administer physiological saline and harmonize into an average suspension It is used for irrigation (oral oral use).
2.2 Animals: ICR mice (18-22 g body weight) and SD rats (120-150 g body weight) were provided by the Faculty of Zoology at Peking University. Administration method: Oral.

3. Method and result:
3.1 Resistance effect of fatigue
120 ICR male mice, each weighing 18-22g, divided into 6 groups arbitrarily, administered to the dosage group by 10 times the recommended amount of human body, and administered the same amount of physiological saline to the control group To do. Reagents were administered for 30 consecutive days, and after 30 minutes of the last administration, the mice were allowed to swim in a swimming box, water depth of 30 cm or higher, water temperature of 25 ± 0.5 ° C, and 5% body weight lead on all buttocks, The time from mouse swimming to death is recorded as the mouse swimming time (min). See Table 2 for results. Excellent emergency function for fatigue recovery

3.2, Anoxic resistance experiment 120 male mice, each weighing 18-22g, are divided into 6 groups, 20 groups each. Administer the above-mentioned doses of TOB, SPPDP, No.1, No.2, No.3 to the drug group, administer the same amount of physiological saline to the control group, and after 30 min, add 125 ml of sodium lime to each mouse. Put the mouse in a closed jar and record the death time of the mouse. See Table 3 for results. TOB, SPPDP, No.1, No.2, No.3 have excellent anti-oxygen deficiency emergency functions that can prolong the survival time of mice under anaerobic conditions under normal atmospheric pressure conditions.

3.3, Low-temperature resistance experiment 60 male mice, each weighing 18-22g, are divided into 3 groups arbitrarily, with 20 mice in each group. Daily doses of TOB and SPPDP 10 mg / kg (10 times the recommended human dose 1 mg / kg, question) are administered to two drug groups, and the same amount of physiological saline is administered to the control group for 10 consecutive days. 0.5 hours after the last dose, place in a refrigerator at -4 to -0.5 ° C and observe every 15 min until death. The average survival time of animals is calculated and the results refer to Table 4. It has been explained that after administration of TOB and SPPDP to mice, the survival time can be extended in a cold environment, which has an effect to withstand cold (anti-hypercold).

3.4 High-temperature resistance experiment
Forty male rats, each weighing 120-150 g, were arbitrarily divided into three groups, each group consisting of 15 rats, and normal body temperature was measured before using the drug. Each drug group will receive 10 mg / kg of TOB and SPPDP, and the same volume of saline will be administered to the control group. After 30 min, the animals are placed in a 45 ± 2 ° C. thermostat, removed after 20 min, the temperature is measured again, the temperature difference is recorded, and the results are shown in Table 5. The increase in the body temperature of rats administered with TOB and SPPDP was lower than that of the control group, which indicated that the ability to resist high temperature (anti-high temperature) of SD rats became stronger after using TOB and SPPDP.

3.5, “Yo-Yan” Therapeutic effect on mice A method of manufacturing a model called “YoYu” (Reference: “Methodology of Chinese Pharmacology Research”, Chen Qi, pages 982-984). 105 ICR male mice, each with a body weight of 18-22g, are divided into 7 groups, and 15 mice each. TOB 10 mg / kg daily except 375 mg / kg hydroxyurea was administered daily to the “Yo-Yan” group, and 175 mg / kg hydroxyurea was administered daily to each group other than the normal control group and “Yo-Yan” group. SPPDP, No.1, No.2, No.3 25 mg / kg is administered, and the same amount of physiological saline is administered to the normal control group. Administer irrigated once daily and observe animal death and changes in body weight for a total of 14 days. Then, after removing the mouse spleen and testicles, washing with water repeatedly, sucking with a filter paper until dry, and weigh the wet tissue. The differences between each tissue are compared and the results refer to Table 6.
Two of the 15 animals in the “Yangan” group died, and weight, spleen, and testicular weight were reduced, with a marked difference compared to the normal control group (P <0.01). On the other hand, the group that received 14 days of TOB, SPPDP, No.1, No.2, No.3 had no dead animals, and the body weight, spleen, and testicle weight were close to those of the normal control group. The difference is significant compared to the “Yo-Yan” group (P <0.05).
Thus, TOB, SPPDP, No. 1, No. 2, and No. 3 explain that there is a clear therapeutic effect on the “pure” mice brought about by hydroxyurea.

3.6, Acute Toxicity Test Forty healthy mice are half male and female, weighing 18-22g each. Take TOB12g and polish it to a fine detail, administer saline and harmonize to an average suspension of 40ml. Stomach 1 ml of this into all sputum. (12000mg / 40ml = 300mg / 1ml / 20g / day, 300mg × 50 / 20g × 50 = 15g / kg, 300mg of combination drug added, maximum concentration 600mg / 1ml / 20g, dose per body weight 30g / kg, equivalent to 600 times clinical), the animals were observed daily after administration and observed for a total of 7 days, and all animals were alive without any abnormal expression. Proven to have no toxic side effects.
In addition, the recommended amount of human use is as follows according to the effective amount of half of animal experiments (fatigue resistance, lack of oxygen, anti-high cold, anti-high temperature, energy enhancement effect, anti-cancer, etc.):
Anti-Emergency Function: 100-240mg / person / day Strengthened Strength: 260-300mg / person / day Anti-cancer: 300-600mg / person / day

3.7 Subacute toxicity experiment 40 rats were halves of males and females, each weighing 120-150 g, and these were arbitrarily divided into 4 groups, that is, 3 drug groups with TOB 0.5 g / kg and 1 g respectively. Administer / kg and 2g / kg, and administer the same amount of saline to the other control group. The first irrigation is administered once daily and the experiment is performed for a total of 60 days. Before administration, during the administration period and at the end of the experiment, weigh each body and examine blood, urine regularity, liver function, and kidney function. At the end of the experiment, the animals are dissected and examined for histomorphology on tissues such as heart, liver, spleen, lung, kidney, adrenal gland, stomach, intestine, brain and gonads. The No.1, No.2, No.3 administration groups are for liver, kidney function and heart, liver, spleen, lung, kidney, adrenal gland, stomach, intestine, and brain for routine examination of rat growth, blood and urine. The results showed that the gonads were not affected.

4. Anti-cancer activity test:
4.1 In vitro anticancer activity test The epidermoid cancer A431, human gastric cancer cells (BGC-823) and human colon cancer (HCT) cells will be provided by the Beijing Institute of Oncology. This compound is selected and assayed using the MTT colorimetric method from the National Center for Natural Medicine and Biotechnology Drugs at Peking University School of Medicine.
The experimental results are as follows. :

4.1.1 Experimental method: Tetramethylazooxime salt (MTT) colorimetric method
Basic principle: After MTT enters the cell, it is oxidized to blue formazan as a basic reactant and deposited in or around the cell, and the OD value is measured directly with an enzyme-labeled photometer. I.e. the dose of formazan can be reacted. Thereby, the activity of intracellular granule oxidase can be determined, and the proliferation level of the cell can be reacted indirectly.
Reagent: MTT [3- (4,5-dimethylthiazal-2yl) 2,5-diphenyltetrazolium bromide] was formulated in a phosphate buffer containing 0.5% glucose to make a 1 mg / ml working solution. Stored at 4 ° C protected from light.
Acidified isopropyl is isopropyl containing 0.04 mol / L HCI.
Experimental cell lines: BGC-823 (human gastric cancer cells), HCT (human colon cancer cells), epidermis cancer A431, NFC (normal adult fiber cells)

4.1.2 Experimental results:

According to the experimental results shown in Table 7, a certain amount of drug (1-5%) has a certain degree of inhibitory effect on the growth of experimental BGC-823, epidermis cancer A431 and HCT cells. However, there is no significant effect on normal adult fiber cell (NFC) growth. TOB (ostrich blood freeze-dried powder) has certainly expressed its value for further development.
The test results show that TOB has no toxic side effects and has an aneurysm rate of 41.39% and 28.24% for human gastric cancer cells (BGC-823) and human colon cancer (HCT) cells, respectively. The aneurysm rate is 47.21% against epidermoid cancer A431, and it has good anticancer activity.

4.4 Internal tumor suppression experiment:
4.2.1 Inhibition of S180 mass in the body: 30 mice are prepared for each sex. Subcutaneous inoculation was performed at 0.2 ml / mouse and inoculated with an S180 body mass homogenization solution (1: 4) corresponding to 3 million cells. On the next day after inoculation, divide into 3 groups of blank control, carrot control and TOB group, and inject each into each by Experiment 2.2 method (oral oral administration). After 9 consecutive days, the animals are sacrificed (slaughtered), the masses are picked and weighed, and the inhibition growth rate is calculated. See Table 8 for results. It is remarkable that TOB suppresses the growth effect of transplantable S180 masses in animals.

5, No.1 No.2 No.3 fatigue resistance test:
The mouse swimming method described in Experiment 3.1 was adopted, 40 ICR male mice and 20 female mice were taken, heavy objects were not carried on the hips of the mice, and other conditions were the same. See Table 9 for results.

6, No.1 No.2 No.3, Wei (Note) Goyo people crowd test:
Select 10 males each in normal and abnormal (explicit) crowds, divide into 4 groups, take 6 days, and the result shows presence / absence action with + and-, or acceleration and rise. Reference is made to Table 10.
Note: “Wei” is a Soyo drug invented by the US company, and is known in Japan as a drug for treating erectile dysfunction under the registered trademark “Viagra”. The main ingredient is sildenafil citrate.

7. Discussion of test results:
The pharmacological action of a vigorous drug is very widespread, and TOB (dry powder) is a compound pharmacological action that has already been extensively studied and discussed in addition to the pharmacological effects of simple drugs such as ginseng, eggplant, and ramie The pharmacological action of simple drugs is almost similar, but because of the combinational relationship between the drugs, the compound action should be a general manifestation of the effects of each drug. The effect was even more pronounced, and this experiment also explained this point.
Chinese medicine doctors believe that the main clinical phenomena of “Yon-Yin” are chills, limb insufficiency or reversal, urine Kiyose, narrow veins, tongue tongue, etc. After animals have used hydroxyurea, they have shrunk and the activity has slowed down, resulting in the phenomenon of “pure and imaginary”, especially when immunosuppressive and clinically “pure and void” patients have low immune function. Are basically the same, and at the same time, this phenomenon of “hypnotized” animals is related to nucleic acid metabolism, especially to low DNA metabolism, so we have hydroxyurea that significantly affects nucleic acid metabolism. I can make a model of “Yo-Yan”, and I think it fits perfectly with clinical practice.
As can be seen in Table 9, Formulations No.1 and No.3 containing male blood TOB have greater resistance to fatigue than Formulation No.2 containing female blood TOB. Compounding agents No. 1 and No. 3 correspond to an average fatigue resistance effect of 2.15 times.
As can be seen in Table 10, a population test was conducted at a high dose and there were no toxic side effects.
TOB and SPPDP all contain active protein. SPPDP is a polypeptide that is mixed with plasma protein that is equal to the amount of serum hydrolyzed by the combination enzyme, and the active polypeptide contained in SPPDP is inhaled into human and animal bodies. It is easy. Therefore, it is estimated that the effect of the SPPDP compound is better than the effect of the TOB compound by the animal test described above.
The most useful combination of TOB and SPPDP was a mixture of ostrich blood freeze-dried powder TOB and active polypeptide hydrolyzed plasma protein with compound enzyme, and other combinations are just helpful .

Table 11 shows the component analysis report of TOB, and Table 12 shows the component analysis test report of the amino acid of TOB. Table 12 is the same as Table 1. For comparison, Table 13 shows the amino acid contents of other animals.

  This shows that ostrich blood is a high protein food containing 21 kinds of amino acids, all of which have reached 91.6%, and taking measurement errors into account, it is over 90%. The amino acid content of the three types of ostrich is almost the same. The ostrich blood freeze-dried powder (abbreviated as TOB dry powder) of the present invention has notable anti-oxygen deficiency, anti-high temperature, anti-high temperature, and three types of emergency functions and energy enhancement effect, and anti-cancer effect can also be expected. Not only that, it is suitable for manufacturing and sales of meat products, health foods, pharmaceuticals, etc., and can be applied in a wide range.

  Here, the manufacturing method of a food strengthening agent and a deliciousness enhancement additive is demonstrated based on FIG. Place 0.5 kg of TOB into a 2 liter reactor and add the following enzymes while stirring. Reaction conditions: PH = 6.5, temperature is 50 ° C. Add 0.5 g of 50 U / g pancreatin, 1 g of 50 U / g papain, and incubate for 4 hours while stirring. Add 2g of activated carbon, decolorize, raise temperature to 90 ° C and continue for 6 minutes. Residual enzyme is inactivated, filtered, and the filtrate is concentrated under reduced pressure. Dry under reduced pressure with circulating temperature not exceeding 70 ° C. The production amount is 50 g and the yield is 10%. It is an off-white needle-shaped polypeptide that can be applied as a food enhancer and a taste enhancing additive.

Next, the manufacturing method of a high-grade liquid seasoning is demonstrated based on FIG. Centrifuge 1kg ostrich TOB to obtain 0.45kg serum for another use. The remaining 0.55 kg of plasma protein, fibrin, hemoglobin, etc. are put into a 2 liter reactor, and the reactor is kept warm in a constant temperature water bath. Reaction conditions: PH = 6, temperature is 55 ° C., papain vitality is 65,000. U / g, enzyme: substrate = 1.25: 100 (enzyme amount is 6.875 g, fibrin etc. is 0.55 kg), and keep warm for 2 hours. Add 1.5g of activated charcoal, decolorize, stir, raise temperature to 100 ° C, continue to inactivate enzyme for 3 minutes, extract air and filter. Discard the filtered residue. Obtain an almost colorless enzyme-hydrolyzed protein solution (polypeptide) and concentrate under reduced pressure so that the specific gravity is 1.2-1.4. The protein recovery rate * is measured, and the recovery rate is 80%. This protein hydrolyzate can be applied as a high-grade liquid seasoning.
* Measurement of protein recovery rate: protein recovery rate (%)
= Total protein content in serum ÷ Substrate protein content X100%.

It is a flowchart figure which shows the manufacturing method of the freeze dried powder of ostrich blood. It is a flowchart figure which shows the manufacturing method of the polypeptide mixture of ostrich blood. It is a flowchart figure which shows the manufacturing method of the food fortifier containing ostrich blood, and a taste enhancement additive. It is a flowchart figure which shows the manufacturing method of the high quality liquid seasoning containing ostrich blood.

Claims (5)

  An ostrich blood freeze-dried powder, which is an active functional agent for ostrich blood, which has fatigue recovery, anti-oxygen deficiency, anti-high temperature, anti-high temperature, anti-cancer effect and energy enhancement effect.   A polypeptide mixture formed by hydrolyzing ostrich blood serum and plasma with specific enzymes at specific pH and temperature, fatigue recovery, anti-oxygen deficiency, anti-high cold, anti-high temperature, anti-cancer effect and energy enhancing effect An active functional agent of ostrich blood characterized by   The compounded enzyme that hydrolyzes the polypeptide mixture according to claim 2 includes at least one of three types of papain, trypsin, and subtilisin, the specific PH is between 6.8 and 7.5, and the specific temperature is 45- The active functional agent for ostrich blood, characterized in that the amount of the specific enzyme used is 0.05 to 1.0% at 55 ° C. The freeze-dried powder according to claim 1 and the polypeptide mixture according to claim 2 all contain an active protein and an active polypeptide, the active protein is 90% or more, and 21 types of amino acids (Table 1 below) And an active functional agent for ostrich blood, wherein the active polypeptide contained in the polypeptide mixture is easily absorbed by human and animal bodies.

The ostrich blood freeze-dried powder according to claim 1 and / or the polypeptide mixture according to claim 2 is mixed at any of the following blending ratios, and is mainly useful for freeze-dried powder of ostrich blood and the polypeptide mixture: A combination drug of ostrich blood, characterized in that other combination drugs are useful in cooperation.
Formulation 1: 400 mg ostrich blood freeze-dried powder according to claim 1, 200 mg coconut extract, 200 mg Western ginseng extract, 200 mg mulberry extract.
Formulation 2: 400 mg of freeze-dried powder of ostrich blood according to claim 1, 200 mg of extract of dried flower beans, 200 mg of extract of western ginseng, 200 mg of extract of lees
Formulation 3: 400 mg of ostrich blood freeze-dried powder according to claim 1, 200 mg of coconut extract, 200 mg of extract of Western ginseng, 200 mg of spiral algae powder.
Formulation 4: 400 mg of the polypeptide mixture according to claim 2, 200 mg of coconut palm extract, 200 mg of Western ginseng extract, and 200 mg of mulberry extract.
Formulation 5: 400 mg of the polypeptide mixture according to claim 2, 200 mg of dried flower bean extract, 200 mg of extract of Western ginseng, 200 mg of extract of nectar.
Formulation 6: 400 mg of the polypeptide mixture according to claim 2, 200 mg of coconut extract, 200 mg of extract of Western ginseng, and 200 mg of spiral algae powder.
Formulation 7: 200 mg ostrich blood freeze-dried powder according to claim 1, 200 mg polypeptide mixture according to claim 2, 200 mg coconut palm extract, 200 mg Western ginseng extract, mulberry fruit extract Extract extract is 200mg.
Formulation 8: 200 mg of freeze dried powder of ostrich blood according to claim 1, 200 mg of the polypeptide mixture according to claim 2, 200 mg of extract of dried flower beans, 200 mg of extract of Western ginseng, Extract extract is 200mg.
Formulation 9: 200 mg of freeze dried powder of ostrich blood according to claim 1, 200 mg of the polypeptide mixture according to claim 2, 200 mg of extract of eggplant, 200 mg of extract of western ginseng, spiral algae powder 200 mg.

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