JP2006010770A5

JP2006010770A5 -

Info

Publication number: JP2006010770A5
Application number: JP2004183925A
Authority: JP
Filing date: 2004-06-22
Publication date: 2007-09-06
Anticipated expiration: 2024-06-22

Claims

Retracting means for moving the stage or the objective lens to the retracted position;
Return means for moving the first member, the specimen, and the second member to the return position, the stage or the objective lens on which the first member, the specimen, and the second member are stacked in this order. When,
Luminance information acquisition means for acquiring luminance information of reflected light from the first member and the second member during the movement to the return position;
An observation range determination unit that determines an observation range in the Z direction based on the luminance information acquired by the luminance information acquisition unit;
A laser scanning microscope apparatus comprising:

Limiting the movable range of the stage or the objective lens according to the observation range in the Z direction determined by the observation range determination means,
The laser scanning microscope apparatus according to claim 1.

Move the stage or objective lens to the retracted position,
The first member, the specimen, and the second member are moved to the return position, the stage or the objective lens placed in the order of the first member, the specimen, and the second member,
During the movement to the return position, the brightness information of the reflected light from the first member and the second member is acquired,
Determining the observation range in the Z direction based on the acquired luminance information;
A method of controlling a laser scanning microscope apparatus, characterized in that:

On the computer,
A function of moving the stage or the objective lens to the retracted position;
A return function for moving the first member, the sample, and the second member to the return position, and the stage or the objective lens on which the first member, the sample, and the second member are stacked in this order. When,
A luminance information acquisition function for acquiring luminance information of reflected light from the first member and the second member during the movement to the return position;
An observation range determination function for determining an observation range in the Z direction based on the acquired luminance information;
A control program for a laser scanning microscope apparatus for realizing the above.

The laser scanning microscope apparatus according to claim 1, wherein the luminance information acquisition unit acquires luminance information for a certain point at each Z position during movement to the return position.

Scanning means for scanning the sample with light irradiated to obtain the luminance information;
The luminance information acquisition means scans the light one-dimensionally or two-dimensionally by the scanning means at each Z position during movement to the return position, and acquires the average luminance of the scanned line or region as the luminance information The laser scanning microscope apparatus according to claim 1, wherein:

Scanning means for scanning the sample with light irradiated to obtain the luminance information;
The luminance information acquisition unit performs line scanning of the light on two lines on the sample by the scanning unit at each Z position during movement to the return position. Luminance information is acquired, The laser scanning microscope apparatus of Claim 1 or 2 characterized by the above-mentioned.

From the acquired luminance information for each of a plurality of different XY positions, an observation range in the Z direction at each position is obtained, and an inclination of the stage is obtained from the difference between them, and the Z in consideration of the obtained inclination is obtained. 8. The laser scanning microscope apparatus according to claim 7, wherein a direction observation range is set.

The wavelength of light emitted to the sample for the luminance information acquisition unit to acquire the luminance information is different from the wavelength of light irradiated to the sample for actual observation of the sample. Item 3. The laser scanning microscope apparatus according to Item 1 or 2.