JP2006000113A - Gene used for diagnosis of herbal treatment, and method for using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a gene enabling an objective, highly reliable and reproducible diagnosis by effectively utilizing information of human genome sequence in herbal treatment not depending on the empirical rule and degree of experience of a doctor and to provide a technology of its use. <P>SOLUTION: Genes varying expression depending on the appearance or disappearance of a specific mark expressing total pathological condition based on herbal treatment, dosing with a specific Chinese medicine and/or effectiveness of a specific Chinese medicine are identified by an exhaustive analysis which effectively utilizes the human genome sequence information. The diagnosis of evaluation of the mark in the herbal treatment and/or selection of Chinese medicine, etc., are objectively performed by measuring varying expression of genes in a subject by using a plurality of genes selected from the identified genes. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a gene used for diagnosis in Kampo medicine and a method for using the same, and more particularly to a gene technique effective in diagnosis such as evaluation of “certification” and evaluation of the efficacy of Kampo medicine in Kampo medicine.

  Modern medicine is basically a medical system based on science developed in the West, and has been making remarkable progress in the 20th century, supported by the development of various pharmaceuticals and medical technologies. Yes.

  Western medicine is characterized as a field of science that attempts to solve problems with two elements of objectivity and logic. It separates humans into spirits and bodies and treats them separately, and the body as an organ. They are divided into individual parts, and further subdivided to consider the human body as a collection of fine parts.

  When a person becomes ill, the emphasis is placed on investigating what kind of damage the organs and cells that are parts of the body are, and the cause of the damage.

  In Western medicine, treatments that cause disease, such as prescribing antibacterial drugs against bacteria, are controlled directly so that the test value falls within the standard value, or against cancer and tumors. The treatment is targeted to a specific target, such as removing the disease by performing surgery.

  In such treatment by Western medicine, there is a strong tendency to focus on test results rather than complaints from patients, and even if a patient complains of `` hardness '' of the body, the cause cannot be determined objectively by examination etc. For example, it is difficult to find a means to solve the problem.

  In recent years, lifestyle-related diseases such as diabetes, hypertension and obesity have become a problem. These illnesses cannot be identified as a single cause, and it is difficult to directly attack the cause of the illness or to focus on a specific target like Western medicine.

  That is, these diseases such as lifestyle-related diseases are not a failure of parts of the human body, but a failure of the entire human body, that is, the system. In the case of a disease related to the whole system of human beings, it is not possible to replace the whole system just because it is out of tune.

  In this way, in the modern society where we are facing an era of disease structure where we cannot find an appropriate response based on Western medical thinking alone, we have set the ultimate goal of treatment as “patient satisfaction” Kampo medicine, which treats patients with a different approach from medicine, is attracting attention as being able to provide treatment that covers a wide range of fields where patients are not satisfied with Western medicine treatment.

  Currently, more than 70% of doctors treat Chinese medicine on a daily basis. In addition, under the guidance of the government, Kampo classes were always included in the educational curriculum from university students in 2002.

  Kampo medicine is a medicine that has been developed by the traditional medicine that came from China and has been devised and improved in Japan, and as listed below, the Western medicine that is the mainstream of the above-mentioned modern medicine is listed below. Have different concepts and systems.

(1) Unlike traditional Western medicine, Kampo medicine captures the pathophysiology and constitution of the entire body, including the mind, without thinking about the human mind and body separately, or thinking about the body in parts. Diagnose it as a “proof” (show), which means an instruction of the name and prescription unique to Kampo medicine.

(2) In Kampo medicine, inquiring about the patient's subjective symptoms in detail and improving the poor feeling felt by the patient occupies a large part of the therapeutic purpose. In the examination, not only the symptoms of the patient's body but also the mental state is taken.

(3) In Kampo medicine, we treat the disease as a state where the balance of the whole body has been lost, and place the basis of treatment in adjusting the balance of the body, thereby improving the natural healing power that humans naturally have. To recover.

(4) According to recent genome analysis, it has been said that it is “custom-made medicine” or “tailor-made medicine”, but in Kampo medicine, it is not a treatment based on the disease name as in Western medicine. Even if they have the same disease name, Kampo medicine has already been offering “custom-made medicine” or “tailor-made medicine” for 3,000 years ago to select and prescribe Chinese medicines that match the “testimony” of the individual patient. It can be said that it has come.

(5) In Kampo medicine, the semi-health state that can be said to be the pre-stage of the disease, such as stiff shoulders and constipation, is regarded as “undisease” (Mibyo), and treatment to improve the body in this semi-health state is performed to prevent the disease . This is one of the fundamental and major differences from Western medicine, which is mainly about coping with illness.

(6) Kampo medicine (also referred to as herbal medicine), a medicine in Kampo medicine, is a complex mixture containing a number of medicinal ingredients, consisting of several herbal medicines such as plants, minerals and animal proteins. The actions of multiple herbal medicines work together on the entire body.

(7) Herbal medicine is said to act on emotions such as sadness, anger, sorrow, surprise, and joy, and is said to be effective for mental illnesses such as depression and neurosis, which are Western medical names. The

  In this way, Kampo medicine is not good at acute acute symptoms and the conditions that require surgery, which is a specialty of Western medicine, but it is a disease in Western medicine, such as coldness and fatigue. Treatment of patients who have no concept and are difficult to make a diagnosis that is directly related to treatment, elderly people, etc. who have weak physical strength and are likely to have side effects of drugs, or patients who have multiple illnesses and have many types of drugs in Western medicine It specializes in the treatment of this and is attracting attention as a medical supplement to Western medicine.

  By the way, in Kampo medicine which has a complementary effect with such Western medicine, there are the following major problems.

  That is, in recent years, in the field of medicine, the idea of EBM (Evidence-Based Medicine), that is, the effectiveness of treatment should be confirmed through clinical trials and research, and treatment should be based on that. The idea that there is a global trend, and Kampo medicine is no exception.

  Kampo medicine has a long history of 3,000 years, and during that time, Kampo medicine has been used by countless patients, and it has few side effects and only effective Kampo medicines are inherited. It remains until now. Moreover, although Kampo medicine does not have statistically organized data, it has been systematized based on such many years of clinical experience and can be said to be a medicine based on a solid basis. In this sense, Kampo medicine is also scientific.

  On the other hand, however, even in Kampo medicine, which can be said to be a vast amount of empirical knowledge, when Western statistical methods are used, no results are obtained for oriental medical treatment. In fact, even if the disease and disease name are the same, if the "proof" does not match, the Kampo medicine will not work. For this reason, from the viewpoint of Western medicine, the grounds are considered to be ineffective, and the Kampo medicine has no grounds. It is a fact that cannot be denied.

  In other words, as described above, Kampo medicine is characterized by the fact that it resolves multiple symptoms, unlike Western medicine, which aims to resolve and resolve one disease state. The Kampo medical examination is performed by a procedure in which a Kampo doctor first evaluates and judges the patient's `` proof '', prescribes the appropriate Kampo medicine based on it, makes the patient take it according to the dosing schedule, and determines the effect. Is called.

  Here, “certification” (show), which means Kampo medicine's unique diagnosis name and prescription instructions, is a symptom pattern that appears in the patient, as will be described later, and consists of genetic and environmental factors. It appears as the sum of countless patient signs such as pulse, tongue condition, complexion, abdominal stiffness, etc. Is comprehensively judged by "four examinations" (Shishin).

  “Four medical checkups” means “visiting” (bowsin) to judge the patient's condition by vision, “hearing” (Bunshin) to judge the patient's condition by hearing and olfaction, “Interview” (Monshin), which is the “cutting” (Sessin) to determine the patient's condition by actually touching the patient's body with the hand. Based on rules of thumb.

  In this way, Kampo diagnosis is based on the evaluation of “proof” that is completely dependent on the subjectivity and empirical rules of Kampo doctors. The short doctors are not able to select the correct Kampo medicines, which is a cause of misunderstanding and mistrust of Kampo medicine and giving many patients the benefits of Kampo medicines. It was also the cause of the indication that there was no.

  Therefore, the realization and establishment of an objective “certification” evaluation method has traditionally been the most important issue for the reliability and reproducibility of Kampo medicine. It has been repeated. For example, by using a pulse wave meter or pressure pulse pulsimeter for pulse diagnosis, or by using an ultrasonic diagnostic imaging device or a vibrotactile sensor for abdominal examination, a little proof of high subjectivity can be obtained. However, an attempt was made to make it objective, but it has not yet achieved sufficient results.

  In other words, “proof” is influenced by the influence of genetic factors and environmental factors, so it is universally applied to single physiological tests and general blood tests performed from the body surface. It was difficult or impossible to have sex.

In addition, proposals have been made to give objective diagnostic indices that can be used by non-experts in Kampo medicine by digitizing and evaluating a database based on the vast knowledge and experience of skilled Kampo doctors. However (see Patent Document 1), the quantification is based on “subjectivity” itself, and does not have true objectivity.
JP 2002-288340 A

  The present invention has been made in view of such conventional problems, and the object of the present invention is to effectively utilize human genome sequence information, so that the rule of thumb and skill level of a doctor can be obtained in Kampo medicine. Independently, it is an object to provide a gene for enabling an objective, reliable and reproducible diagnosis and a technique for using the gene.

In order to achieve the above object, the present invention provides:
(1) a gene used for diagnosis of Kampo medicine, a fragment thereof, or a complementary sequence thereof, characterized in that the expression varies depending on a specific measurement standard of Kampo medicine;

(2) The gene used for the diagnosis of Kampo medicine according to (1) above, wherein the measurement standard is the appearance / disappearance of a specific “test” evaluated and determined by Kampo doctors based on Kampo medicine , Fragments thereof or complementary sequence strands thereof;

(3) The above-mentioned “certification” is a “certification” of a specific Chinese medicine, the gene used for the diagnosis of Kampo medicine according to (2), a fragment thereof, or a complementary sequence chain thereof;

(4) The gene used for the diagnosis of Kampo medicine according to (3), a fragment thereof or a complementary sequence chain thereof, wherein the “proof” of the above-mentioned Chinese medicine is “proof of Koshosan”;

(5) The gene used for the diagnosis of Kampo medicine according to (3) above, wherein the “proof” of the above-mentioned Chinese medicine is “proof of Hansatsu Kobokuto”;

(6) The gene used for diagnosis of Kampo medicine according to (1), a fragment thereof, or a complementary sequence chain thereof, wherein the measurement standard is taking a specific Chinese medicine;

(7) The above-mentioned measurement standard is the effectiveness of a specific Chinese medicine, the gene used for the diagnosis of Chinese medicine according to claim 1, a fragment thereof, or a complementary sequence chain thereof;

(8) The gene used for the diagnosis of Kampo medicine according to (6) or (7), wherein the herbal medicine is Kasousan, or a complementary sequence chain thereof;

(9) The gene used for the diagnosis of Kampo medicine according to the above (6) or (7), wherein the Chinese herb medicine is Hanka Kobokuto, or a complementary sequence chain thereof;

(10) The above measurement standard is a combination of at least two of the appearance / disappearance of a specific “certification” evaluated and determined based on Kampo medicine, the taking of a specific Chinese medicine, and the effectiveness of a specific Chinese medicine. The gene used for the diagnosis of Chinese medicine according to the above (1), a fragment thereof or a complementary sequence chain thereof;

(11) The gene is a gene comprising the base sequence described in SEQ ID NO: 1 to SEQ ID NO: 49 or a part thereof in the sequence listing, and NCBI Unigene database SEQ ID NO: Hs.487001, Hs.436042, and Hs.61714. The gene used for the diagnosis of Chinese medicine according to the above (4) or (8), which is any one or more of the described genes, a fragment thereof, or a complementary sequence chain thereof, the fragment thereof Or their complementary strands;

(12) The above genes are represented by the sequence numbers Hs.180911, Hs.294176, Hs.53250, Hs.282331, Hs.440606, Hs.86538, Hs.61232, Hs.24884, Hs.110299, NCBI Unigene database. Hs.303523, Hs.115617, Hs.3321, Hs.334527, Hs.161640, Hs.225943, Hs.323213, Hs.186582, Hs.24583, Hs.234775, Hs.512633, Hs.377022, Hs. 29189, Hs.262480, Hs.8852, Hs.527699, Hs.445241, Hs.437457, Hs.436628, Hs.115497, Hs.497707, Hs.150826, Hs.266902, Hs.442801, Hs.8022, Hs.432379, Hs.460471, Hs.434195, Hs.145156, Hs.396566, Hs.367761, Hs.12902, Hs.297997, Hs.183390, Hs.479572, Hs.450425, Hs.283085, Hs. 103388, Hs.18213, Hs.378736, Hs.389964, Hs.387258, Hs.355820, Hs.210850, Hs.158318, Hs.157461, Hs.17860, Hs.355711, Hs.74376, Hs.111726, Hs. 54468, Hs. 75236, Hs. 193228, Hs. 512660, Hs. 130685, Hs. 272493, Hs. 414091, Hs. 113912, Hs. 128204, Hs. 115181, Hs. 86088, Hs. 431156, Hs. 115830, Hs.37058, Hs.125507, Hs.376586, Hs.97808, Hs.49 3514, Hs.412355, Hs.97933, Hs.288727, Hs.499520, Hs.53996, Hs.144028, Hs.131555, Hs.232779, Hs.382347, Hs.87224, Hs.382870, Hs.82535, Hs.235498, Hs.438482, Hs.129778, Hs.22575, Hs.98967, Hs.320086, Hs.412022, Hs.259498, Hs.445025, Hs.334022, Hs.51120, Hs.302109, Hs. 268744, Hs.152664, Hs.153203, Hs.201269, Hs.448749, Hs.408843, Hs.234790, Hs.147305, Hs.436494, Hs.12840, Hs.158963, Hs.283880, Hs.476396, Hs.148956, Hs.326012, Hs.437137, Hs.406763, Hs.150276, Hs.408195, Hs.496614, Hs.251573, Hs.205457, Hs.132275, Hs.233325, Hs.343666, Hs. 49753, Hs.432815, Hs.443683, Hs.143907, Hs.22116, Hs.336224, Hs.22573, Hs.66, Hs.234774, Hs.130310, Hs.25363, Hs.78465, Hs.367888, Hs.127059, Hs.140821, Hs.511968, Hs.432445, Hs.350445, Hs.48695, Hs.430138, Hs.99607, Hs.213493, Hs.8345, Hs.502577, Hs.57876, Hs. 100299, Hs.458408, Hs.406639, Hs.512760, Hs.123457, Hs.326561, Hs.446702, Hs.89121, Hs.17681, Hs.148074, Hs.97714 Hs.2936, Hs.158357, Hs.499247, Hs.153692, Hs.406234, Hs.241385, Hs.271809, Hs.278568, Hs.254122, Hs.318791, Hs.189482, Hs.282938, Hs. 390808, Hs.2149, Hs.423112, Hs.301715, Hs.55016, Hs.99120, Hs.511985, Hs.359978, Hs.204238, Hs.283506, Hs.447877, Hs.137674, Hs.266738, Hs.302145, Hs.399966, Hs.149098, Hs.431010, Hs.222312, Hs.2207, Hs.25094, Hs.79881, Hs.116651, Hs.25328, Hs.501480, Hs.8346, Hs. 157796, Hs.21239, Hs.508901, Hs.144875, Hs.40735, Hs.341806, Hs.125423, Hs.511880, Hs.433827, Hs.307044, Hs.306697, Hs.134807, Hs.387367, Hs.335355, Hs.349109, Hs.418105, Hs.376337, Hs.137574, Hs.44077, Hs.44899, Hs.184945, Hs.114088, Hs.376025, Hs.21258, Hs.207407, Hs. 117938, Hs.169071, Hs.385986, Hs.284153, Hs.95582, Hs.21380, Hs.418083, Hs.91625, Hs.397167, Hs.437046, Hs.14468, Hs.26309, Hs.479080, Hs.352530, Hs.73958, Hs.171025, Hs.121001, Hs.11713, Hs.280311, Hs.8535, Hs.283326, Hs.407474, Hs.451697, Hs.14 732, Hs.73890, Hs.42650, Hs.7327, Hs.287640, Hs.194659, Hs.191381, Hs.406789, Hs.227098, Hs.7934, Hs.512643, Hs.256583, Hs.408355, Hs.155182, Hs.30340, Hs.178023, Hs.58974, Hs.390568, Hs.185796, Hs.115263, Hs.414251, Hs.119693, Hs.511776, Hs.196530, Hs.157573, Hs. 36761, Hs.223581, Hs.511753, Hs.17170, Hs.448823, Hs.102929, Hs.37616, Hs.511824, Hs.354013, Hs.132380, Hs.371496, Hs.458427, Hs.32967, Hs.143751, Hs.253690, Hs.352, Hs.21177, Hs.406681, Hs.435976, Hs.46679, Hs.148101, Hs.9914, Hs.310230, Hs.376590, Hs.458397, Hs. 58414, Hs.17165, Hs.407015, Hs.470704, Hs.246914, Hs.15420, Hs.436282, Hs.257368, Hs.512739, Hs.387679, Hs.283078, Hs.516782, Hs.109752, Hs.201667, Hs.149504, Hs.398139, Hs.94367, Hs.112208, Hs.395779, Hs.445563, Hs.65135, Hs.187203, Hs.490216, Hs.487918, Hs.436495, Hs. 125571, Hs.519634, Hs.399769, Hs.511987, Hs.26176, Hs.25934, Hs.351825, Hs.283794, Hs.256067, Hs.251699, Hs.436378, One or more of any of the genes described in Hs.523755, Hs.449884, Hs.41154, Hs.54609, Hs.458291, Hs.408336 and Hs.2012, fragments thereof or complementary sequence strands thereof The gene used for the diagnosis of Kampo medicine according to (4) or (8), a fragment thereof or a complementary sequence chain thereof;

(13) The above genes are represented by NCBI Unigene database SEQ ID NOs: Hs.372133, Hs.281004, 7.59 Hs.420563, Hs.376818, Hs.15780, Hs.493450, Hs.306814, Hs.511810, Hs.143241 , Hs.278924, Hs.512632, Hs.367633, Hs.127363, Hs.414110, Hs.434143, Hs.36567, Hs.356605, Hs.414648, Hs.104888, Hs.197435, Hs.410810, Hs .434375, Hs.56279, Hs.125487, Hs.51011, Hs.170953, Hs.66392, Hs.48372, Hs.356664, Hs.420269, Hs.3017, Hs.306811, Hs.93121, Hs.438880 , Hs.302029, Hs.463017, Hs.412559, Hs.17518, Hs.2133, Hs.400380, Hs.75813, Hs.287656, Hs.334347, Hs.411572, Hs.6496, Hs.268764, Hs .385496, Hs.410088, Hs.24835, Hs.446504, Hs.7972, Hs.292057, Hs.435775, Hs.78106, Hs.217754, Hs.153704, Hs.341906, Hs.250830, Hs.68846 , Hs.438712, Hs.99480, Hs.434418, Hs.171980, Hs.376810, Hs.324335, Hs.37936, Hs.512546, Hs.128836, Hs.406330, Hs.302352, Hs.169825, Hs .522765, Hs.526526, Hs.82002, Hs.526394, Hs.512785, Hs .1937, Hs.105700, Hs.122895, Hs.9589, Hs.99439, Hs.334828, Hs.126161, Hs.465202, Hs.310231, Hs.94875, Hs.203557, Hs.444729, Hs.449965 , Hs.122309, Hs.190090, Hs.355714, Hs.153952, Hs.408577, Hs.287370, Hs.185202, Hs.29792, Hs.4845, Hs.27183, Hs.486433, Hs.439463, Hs .194665, Hs.129312, Hs.163682, Hs.73853, Hs.2689, Hs.283900, Hs.162604, Hs.381367, Hs.288218, Hs.203349, Hs.433891, Hs.248065, Hs.440603 , Hs.144913, Hs.199316, Hs.221660, Hs.21554, Hs.510244, Hs.436325, Hs.117979, Hs.356888, Hs.306322, Hs.310431, Hs.356501, Hs.444708, Hs .159609, Hs.18069, Hs.373612, Hs.12802, Hs.508901, Hs.504580, Hs.308242, Hs.128580, Hs.112184, Hs.306298, Hs.343800, Hs.50841, Hs.137516 , Hs.413095, Hs.105640, Hs.144696, Hs.175411, Hs.207407, Hs.406856, Hs.122959, Hs.143792, Hs.170195, Hs.381715, Hs.443292, Hs.56874, Hs .147096, Hs.521267, Hs.196956, Hs.80720, Hs.440953, Hs.352387, Hs.434107, Hs.112720, Hs.1555584, Hs.147352, H s.146329, Hs.463171, Hs.445445, Hs.156892, Hs.212670, Hs.129895, Hs.356076, Hs.439590, Hs.278613, Hs.54697, Hs.388917, Hs.55923, Hs. 460377, Hs.416244, Hs.234786, Hs.129969, Hs.275711, Hs.127842, Hs.302772, Hs.337008, Hs.48480, Hs.121115, Hs.318773, Hs.98564, Hs.276466, Hs.78824, Hs.124502, Hs.325530, Hs.375664, Hs.516311, Hs.367688, Hs.386155, Hs.189174, Hs.159264, Hs.386143, Hs.158446, Hs.209461, Hs. 163484, Hs.300559, Hs.155223, Hs.448979, Hs.368420, Hs.289044, Hs.74088, Hs.93907, Hs.408264, Hs.439168, Hs.437433, Hs.448468, Hs.310194, Hs.45152, Hs.283095, Hs.500957, Hs.431156, Hs.511922, Hs.406549, Hs.288731, Hs.123419, Hs.334702, Hs.114172, Hs.408261, Hs.436657, Hs. 125300, Hs.203, Hs.287499, Hs.102943, Hs.145949, Hs.248290, Hs.418167, Hs.55481, Hs.434181, Hs.275906, Hs.249126, Hs.147256, Hs.178295, Hs.385890, Hs.201800, Hs.96633, Hs.445489, Hs.449650, Hs.102708, Hs.433381, Hs.27263, Hs.512782, Hs.3475 37, Hs.124115, Hs.1270, Hs.349121, Hs.293884, Hs.143943, Hs.12742, Hs.166705, Hs.13209, Hs.77719, Hs.129694, Hs.126906, Hs.151414, Hs.212584, Hs.232819, Hs.41324, Hs.140821, Hs.351593, Hs.12248, Hs.434281, Hs.283506, Hs.66412.18 Hs.2115872.17 Hs.427712.17 Hs.447703, Hs.372208, Hs .379010, Hs.437008, Hs.99311, Hs.464896, Hs.323079, Hs.509439, Hs.440088, Hs.34045, Hs.406073, Hs.455335, Hs.175465, Hs.507549, Hs.127775 , Hs.132888, Hs.8728, Hs.157231, Hs.62863, Hs.169600, Hs.414332, Hs.259403, Hs.101949, Hs.436339, Hs.128668, Hs.380167, Hs.79340, Hs .43125, Hs.2251, Hs.142296, Hs.288155, Hs.399740, Hs.27524, Hs.165464, Hs.123642, Hs.125156, Hs.58561, Hs.418966, Hs.447121, Hs.24734 , Hs.348709, Hs.89436, Hs.2563, Hs.186363, Hs.169470, Hs.6540, Hs.223206, Hs.439169, Hs.133250, Hs.172562, Hs.349705, Hs.405457, Hs .145894, Hs.7360, Hs.163546, Hs.102276, Hs.511781, Hs.283794, Hs.171090, Hs.508459, Hs.276808, Hs.248155 (4) or the above (4), which is one or more of the genes described in Hs.436042, Hs.371212, Hs.221040 and Hs.374990, a fragment thereof or a complementary sequence chain thereof The gene used for the diagnosis of Chinese medicine according to (8), a fragment thereof, or a complementary sequence thereof;

(14) The above genes are represented by NCBI Unigene database SEQ ID NOs: Hs.58323, Hs.160373, Hs.334832, Hs.150136, Hs.272385, Hs.382870, Hs.430919, Hs.387551, Hs.194408, Hs.406961, Hs.164021, Hs.24884, Hs.437211, Hs.436628, Hs.283880, Hs.110299, Hs.390242, Hs.113291, Hs.278894, Hs.90221, Hs.24583, Hs. 266902, Hs.148956, Hs.162282, Hs.103388, Hs.406300, Hs.382347, Hs.413430, Hs.449884, Hs.24889, Hs.440606, Hs.36069, Hs.131255, Hs.369557, Hs.512660, Hs.359981, Hs.283085, Hs.163546, Hs.117938, Hs.74376, Hs.438482, Hs.482392, Hs.320086, Hs.95990, Hs.9469, Hs.108642, Hs. 180016, Hs.479572, Hs.169395, Hs.335319, Hs.442801, Hs.111726, Hs.183390, Hs.449407, Hs.434195, Hs.17546, Hs.127951, Hs.458408, Hs.287374, Hs.150826, Hs.181483, Hs.115467, Hs.232779, Hs.96557, Hs.406722, Hs.201269, Hs.47313, Hs.8852, Hs.283326, Hs.225943, Hs.12902, Hs. 500371, Hs.7296, Hs.430854, Hs.278568, Hs.132275, Hs .66392, Hs.437179, Hs.8850, Hs.98967, Hs.57876, Hs.459828, Hs.310230, Hs.25328, Hs.301956, Hs.144875, Hs.110457, Hs.509265, Hs.60339 , Hs.511880, Hs.446152, Hs.48100, Hs.247975, Hs.479080, Hs.490096, Hs.8346, Hs.131555, Hs.32967, Hs.307003, Hs.127032, Hs.109268, Hs .80720, Hs.266738, Hs.113912, Hs.175780, Hs.497972, Hs.18213, Hs.152664, Hs.459847, Hs.378736, Hs.458427, Hs.36761, Hs.429531, Hs.149797 , Hs.444135, Hs.22575, Hs.272191, Hs.512643, Hs.54713, Hs.820, Hs.77221, Hs.253690, Hs.436282, Hs.181289, Hs.170560, Hs.426734, Hs .334527, Hs.406701, Hs.177434, Hs.511968, Hs.389964, Hs.251699, Hs.207805, Hs.140821, Hs.281004, Hs.435675, Hs.292597, Hs.178715, Hs.161394 , Hs.396332, Hs.302109, Hs.86088, Hs.356794, Hs.449392, Hs.134807, Hs.210850, Hs.18705, Hs.326444, Hs.287453, Hs.130522, Hs.234775, Hs .438555, Hs.171532, Hs.54617, Hs.268843, Hs.11713, Hs.5025, Hs.288727, Hs.412022, Hs.78465, Hs.423112, Hs.497707 , Hs.235390, Hs.33455, Hs.371278, Hs.237642, Hs.234790, Hs.78792, Hs.115181, Hs.115617, Hs.463017, Hs.406789, Hs.378753, Hs.256583, Hs .99430, Hs.525361, Hs.148559, Hs.249181, Hs.23853, Hs.172052, Hs.90821, Hs.444182, Hs.123474, Hs.284310, Hs.137574, Hs.102929, Hs.511985 , Hs.371335, Hs.528103, Hs.355711, Hs.496614, Hs.27621, Hs.183006, Hs.27021, Hs.389571, Hs.227457, Hs.334772, Hs.512760, Hs.53996, Hs .207407, Hs.297997, Hs.512808, Hs.149467, Hs.510932, Hs.272493, Hs.79881, Hs.3052, Hs.415895, Hs.388220, Hs.253193, Hs.124502, Hs.232803 , Hs.269777, Hs.12264, Hs.380763, Hs.147595, Hs.411274, Hs.441481, Hs.376025, Hs.250895, Hs.468922, Hs.161427, Hs.385986, Hs.439760, Hs .313413, Hs.408843, Hs.58974, Hs.430755, Hs.99120, Hs.25363, Hs.287405, Hs.131035, Hs.335355, Hs.259471, Hs.235888, Hs.144527, Hs.414091 , Hs.480174, Hs.445241, Hs.44888, Hs.382683, Hs.439494, Hs.288520, Hs.234027, Hs.130685, Hs.399966, Hs.43 4486, Hs.407002, Hs.333958, Hs.238648, Hs.308045, Hs.196470, Hs.144531, Hs.7327, Hs.83916, Hs.169441, Hs.73890, Hs.432374, Hs.143751, Hs.2074, Hs.158278, Hs.158963, Hs.490216, Hs.103422, Hs.133361, Hs.404220, Hs.169071, Hs.288718, Hs.144130, Hs.336224, Hs.112669, Hs. 159265, Hs.122110, Hs.7835, Hs.407015, Hs.441708, Hs.452398, Hs.439124, Hs.443020, Hs.499520, Hs.356572, Hs.284200, Hs.78944, Hs.512387, Hs.235498, Hs.133082, Hs.471423, Hs.73625, Hs.9914, Hs.355862, Hs.82120, Hs.443392, Hs.401954, Hs.436140, Hs.461482, Hs.433596, Hs. 434103, Hs.385898, Hs.348920, Hs.81848, Hs.432379, Hs.79025, Hs.407162, Hs.197922, Hs.180171, Hs.408200, Hs.444529, Hs.164649, Hs.4224, Hs.460471, Hs.149152, Hs.458397, Hs.124211, Hs.415497, Hs.293917, Hs.253489, Hs.14822, Hs.376590, Hs.179112, Hs.152337, Hs.438300, Hs. 344099, Hs.421986, Hs.432458, Hs.351414, Hs.54460, Hs.349227, Hs.47312, Hs.185796, Hs.26981, Hs.267038, Hs.448 749, Hs.170114, Hs.95582, Hs.352601, Hs.353001, Hs.352617, Hs.414164, Hs.30340, Hs.359978, Hs.298241, Hs.178292, Hs.156232, Hs.406681, Hs.411644, Hs.97933, Hs.137674, Hs.476396, Hs.388877, Hs.155573, Hs.87, Hs.153203, Hs.458434, Hs.10056, Hs.148074 and Hs.377022 Any one or more of these genes, fragments thereof, or complementary sequence chains thereof, the gene, fragment thereof, or these used in the diagnosis of Kampo medicine according to (4) or (8) above Complementary strands of

(15) The above genes are represented by NCBI Unigene database SEQ ID NOs: Hs.436450, Hs.278613, Hs.411239, Hs.445072, Hs.123515, Hs.17518, Hs.128668, Hs.280226, Hs.511758, Hs.233437, Hs.187802, Hs.61929, Hs.406741, Hs.245431, Hs.20082, Hs.29798, Hs.222, Hs.162089, Hs.37936, Hs.144913, Hs.166351, Hs. 313, Hs.522901, Hs.450230, Hs.89679, Hs.323966, Hs.439168, Hs.159629, Hs.186363, Hs.172052, Hs.250723, Hs.356605, Hs.376407, Hs.188021, Hs.386264, Hs.40510, Hs.408067, Hs.58009, Hs.222909, Hs.113227, Hs.350800, Hs.377070, Hs.178295, Hs.222746, Hs.351811, Hs.440088, Hs. 490397, Hs.344400, Hs.361748, Hs.283045, Hs.27258, Hs.25328, Hs.350765, Hs.103665, Hs.194725, Hs.327621, Hs.408629, Hs.411347, Hs.489592, Hs.386236, Hs.446451, Hs.105115, Hs.45207, Hs.91283, Hs.178499, Hs.444882, Hs.381370, Hs.369994, Hs.93883, Hs.306230, Hs.413924, Hs. 504809, Hs.84665, Hs.389724, Hs.211587, Hs.1560, Hs.406 733, Hs.306812, Hs.348745, Hs.106688, Hs.129895, Hs.388917, Hs.368746, Hs.413525, Hs.166011, Hs.94865, Hs.250472, Hs.434485, Hs.267038, Hs.445426, Hs.154084, Hs.23317, Hs.75360, Hs.127405, Hs.155584, Hs.47504, Hs.90318, Hs.128499, Hs.283082, Hs.449006, Hs.309365, Hs. 436602, Hs.324335, Hs.351816, Hs.306992, Hs.512632, Hs.443260, Hs.348709, Hs.426324, Hs.227656, Hs.435092, Hs.272200, Hs.347537, Hs.271269, Hs.424414, Hs.707, Hs.248204, Hs.168355, Hs.58559, Hs.407817, Hs.447162, Hs.144870, Hs.435398, Hs.146025, Hs.406485, Hs.326559, Hs. 258536, Hs.169825, Hs.1270, Hs.325335, Hs.335877, Hs.421280, Hs.443120, Hs.389638, Hs.124673, Hs.150138, Hs.476618, Hs.438482, Hs.652, Hs.199372, Hs.493952, Hs.371212, Hs.25156, Hs.301956, Hs.511810, Hs.151408, Hs.157144, Hs.159525, Hs.94327, Hs.449791, Hs.320522, Hs. 123114, Hs.446376, Hs.250666, Hs.201554, Hs.351818, Hs.189575, Hs.132270, Hs.36793, Hs.23367, Hs.104613, Hs.2 54006, Hs.133995, Hs.395205, Hs.476835, Hs.513009, Hs.257584, Hs.5462, Hs.96149, Hs.374720, Hs.46791, Hs.127473, Hs.91813, Hs.271640, Hs.379010, Hs.516739, Hs.376657, Hs.121490, Hs.352240, Hs.203557, Hs.228982, Hs.122115, Hs.112928, Hs.436975, Hs.387258, Hs.433197, Hs. 159553, Hs.437, Hs.288911, Hs.440953, Hs.20315, Hs.104859, Hs.38260, Hs.199316, Hs.511791, Hs.282275, Hs.249126, Hs.163484, Hs.297604, Hs.73853, Hs.27335, Hs.26176, Hs.92683, Hs.212584, Hs.372616, Hs.153647, Hs.121115, Hs.87306, Hs.318775, Hs.353114, Hs.167679, Hs. 415209, Hs.55209, Hs.143600, Hs.156007, Hs.444960, Hs.234016, Hs.283011, Hs.512778, Hs.37107, Hs.414332, Hs.164680, Hs.74115, Hs.422120, Hs.104133, Hs.21861, Hs.249184, Hs.375054, Hs.436424, Hs.302754, Hs.297452, Hs.165387, Hs.493450, Hs.306094, Hs.414110, Hs.118057, Hs. 840, Hs.2207, Hs.343154, Hs.445772, Hs.132272, Hs.59892, Hs.380519, Hs.442936, Hs.274548, Hs.72910, Hs.49433 Hs.26663, Hs.155814, Hs.212892, Hs.408488, Hs.37125, Hs.375870, Hs.440695, Hs.341906, Hs.370622, Hs.314141, Hs.118631, Hs.115365, Hs. 238964, Hs.331491, Hs.194714, Hs.434375, Hs.127012, Hs.306622, Hs.211298, Hs.264330, Hs.347534, Hs.148767, Hs.41270, Hs.283096, Hs.110637, Hs.76152, Hs.334, Hs.55412, Hs.497743, Hs.276808, Hs.114599, Hs.461085, Hs.282787, Hs.131834, Hs.21838, Hs.492218, Hs.248114, Hs. 501321, Hs.512787, Hs.268700, Hs.313948, Hs.211477, Hs.2053, Hs.21388, Hs.507546, Hs.1501, Hs.248139, Hs.283779, Hs.511992, Hs.406984, Hs.145680, Hs.403836, Hs.287656, Hs.53263, Hs.257541, Hs.413265, Hs.333742, Hs.156346, Hs.441287, Hs.131101, Hs.436983, Hs.256036, Hs. 420036, Hs.440387, Hs.122309, Hs.479817, Hs.195614, Hs.355584, Hs.65248, Hs.512782, Hs.363315, Hs.43107, Hs.233950, Hs.445445, Hs.82316, Any one or more of the genes described in Hs.148990, Hs.158798, Hs.189067 and Hs.90797 Gene, fragment or herbal gene used in the diagnosis of medical, fragment or complementary sequence chain of thereof according to the above (4) or (8), which is a complementary sequences chain;

(16) The gene is any one or two or more genes, fragments thereof, or complementary sequence chains of the genes including the nucleotide sequence described in SEQ ID NO: 290 to SEQ ID NO: 338 in the sequence listing or a part thereof The gene used for the diagnosis of Kampo medicine according to (5) or (9), a fragment thereof, or a complementary sequence chain thereof;

(17) A plurality of the genes according to any one of (1), (2), (3), (6) and (10) above, fragments thereof or complementary sequence chains thereof are immobilized on a support. A gene expression analysis platform characterized by comprising:

(18) The gene, a fragment thereof or a complementary sequence chain thereof is comprehensively analyzed using a fluorescent differential display method or a DNA microarray method, and at least five genes, fragments thereof, A base for gene expression analysis according to the above (17), which is a complementary sequence of these;

(19) A gene expression analysis method comprising using the gene expression analysis platform according to (17) or (18) above;

(20) Using the gene according to any one of (1), (2), (3), (6) and (10) above, a fragment thereof or a complementary sequence chain thereof as a target gene, This is a method for evaluating medical "proof", which measures the expression state of a target gene in a measurement sample obtained from a subject, and uses the measurement result as the expression state of the target gene in a comparative sample serving as an evaluation standard. An evaluation method of “proof” in Chinese medicine, characterized by evaluating the presence or absence of expression of the “proof” of the subject by comparison;

(21) The expression of the target gene varies depending on the appearance and disappearance of the “proof of Koshosan”, the use of Kososan and / or the effectiveness of a specific Chinese medicine (20) ) Evaluation method of “certification” in Chinese medicine

(22) The target gene is a gene containing the base sequence described in SEQ ID NO: 1 to SEQ ID NO: 49 in the sequence listing or a part thereof, and the sequence numbers Hs.487001, Hs.436042 and Hs.61714 in the NCBI Unigene database. Any one or more of the genes described in 1), a fragment thereof, or a complementary sequence chain thereof;

(23) The above-mentioned target gene is the sequence number Hs.180911, Hs.294176, Hs.53250, Hs.282331, Hs.440606, Hs.86638, Hs.61232, Hs.24832, Hs.110299 in the NCBI Unigene database. , Hs.303523, Hs.115617, Hs.3321, Hs.334527, Hs.161640, Hs.225943, Hs.323213, Hs.186582, Hs.24583, Hs.234775, Hs.512633, Hs.377022, Hs .29189, Hs.262480, Hs.8852, Hs.527699, Hs.445241, Hs.437457, Hs.436628, Hs.115497, Hs.497707, Hs.150826, Hs.266902, Hs.442801, Hs.8022 , Hs.432379, Hs.460471, Hs.434195, Hs.145156, Hs.396566, Hs.367761, Hs.12902, Hs.297997, Hs.183390, Hs.479572, Hs.450425, Hs.283085, Hs .103388, Hs.18213, Hs.378736, Hs.389964, Hs.387258, Hs.355820, Hs.210850, Hs.158318, Hs.157461, Hs.17860, Hs.355711, Hs.74376, Hs.111726 , Hs.54468, Hs.75236, Hs.193228, Hs.512660, Hs.130685, Hs.272493, Hs.414091, Hs.113912, Hs.128204, Hs.115181, Hs.86088, Hs.431156, Hs .115830, Hs.37058, Hs.125507, Hs.376586, Hs.97808 Hs.493514, Hs.412355, Hs.97933, Hs.288727, Hs.499520, Hs.53996, Hs.144028, Hs.131555, Hs.232779, Hs.382347, Hs.382224, Hs.382870, Hs. 82535, Hs.235498, Hs.438482, Hs.129778, Hs.22575, Hs.98967, Hs.320086, Hs.412022, Hs.259498, Hs.445025, Hs.334022, Hs.51120, Hs.302109, Hs.268744, Hs.152664, Hs.153203, Hs.201269, Hs.448749, Hs.408843, Hs.234790, Hs.147305, Hs.436494, Hs.12840, Hs.158963, Hs.283880, Hs. 476396, Hs.148956, Hs.326012, Hs.437137, Hs.406763, Hs.150276, Hs.408195, Hs.496614, Hs.251573, Hs.205457, Hs.132275, Hs.233325, Hs.343666, Hs.49753, Hs.432815, Hs.443683, Hs.143907, Hs.22116, Hs.336224, Hs.22573, Hs.66, Hs.234774, Hs.130310, Hs.25363, Hs.78465, Hs. 367888, Hs.127059, Hs.140821, Hs.511968, Hs.432445, Hs.350765, Hs.48695, Hs.430138, Hs.99607, Hs.213493, Hs.8345, Hs.502577, Hs.57876, Hs.100299, Hs.458408, Hs.406639, Hs.512760, Hs.123457, Hs.326561, Hs.446702, Hs.89121, Hs.17681, Hs.148074, Hs. 97714, Hs.2936, Hs.158357, Hs.499247, Hs.153692, Hs.406234, Hs.241385, Hs.271809, Hs.278568, Hs.254122, Hs.318791, Hs.189482, Hs.282938, Hs.390808, Hs.2149, Hs.423112, Hs.301715, Hs.55016, Hs.99120, Hs.511985, Hs.359978, Hs.204238, Hs.283506, Hs.447877, Hs.137674, Hs. 266738, Hs.302145, Hs.399966, Hs.149098, Hs.431010, Hs.222312, Hs.2207, Hs.25094, Hs.79881, Hs.116651, Hs.25328, Hs.501480, Hs.8346, Hs.157796, Hs.21239, Hs.508901, Hs.144875, Hs.40735, Hs.341806, Hs.125423, Hs.511880, Hs.433827, Hs.307044, Hs.306697, Hs.134807, Hs. 387367, Hs.335355, Hs.349109, Hs.418105, Hs.376337, Hs.137574, Hs.44077, Hs.44899, Hs.184945, Hs.114088, Hs.376025, Hs.21258, Hs.207407, Hs.117938, Hs.169071, Hs.385986, Hs.284153, Hs.95582, Hs.21380, Hs.418083, Hs.91625, Hs.397167, Hs.437046, Hs.14468, Hs.26309, Hs. 479080, Hs.352530, Hs.73958, Hs.171025, Hs.121001, Hs.11713, Hs.280311, Hs.8535, Hs.283326, Hs.407474, Hs.451697 Hs.14732, Hs.73890, Hs.42650, Hs.7327, Hs.287640, Hs.194659, Hs.191381, Hs.406789, Hs.227098, Hs.7934, Hs.512643, Hs.256583, Hs. 408355, Hs.155182, Hs.30340, Hs.178023, Hs.58974, Hs.390568, Hs.185796, Hs.115263, Hs.414251, Hs.119693, Hs.511776, Hs.196530, Hs.157573, Hs.36761, Hs.223581, Hs.511753, Hs.17170, Hs.448823, Hs.102929, Hs.37616, Hs.511824, Hs.354013, Hs.132380, Hs.371496, Hs.458427, Hs. 32967, Hs.143751, Hs.253690, Hs.352, Hs.21177, Hs.406681, Hs.435976, Hs.46679, Hs.148101, Hs.9914, Hs.310230, Hs.376590, Hs.458397, Hs.58414, Hs.17165, Hs.407015, Hs.470704, Hs.246914, Hs.15420, Hs.436282, Hs.257368, Hs.512739, Hs.387679, Hs.283078, Hs.516782, Hs. 109752, Hs.201667, Hs.149504, Hs.398139, Hs.94367, Hs.112208, Hs.395779, Hs.445563, Hs.65135, Hs.187203, Hs.490216, Hs.487918, Hs.436495, Hs.125571, Hs.519634, Hs.399769, Hs.511987, Hs.26176, Hs.25934, Hs.351825, Hs.283794, Hs.256067, Hs.251699, Hs.436 378, Hs.523755, Hs.449884, Hs.41154, Hs.54609, Hs.458291, Hs.408336 and Hs.2012 One or more genes, fragments thereof or complements thereof The evaluation method of “certification” in Kampo medicine according to (21) above, which is a sequence chain;

(24) The above target genes are represented by NCBI Unigene database SEQ ID NOs: Hs.372133, Hs.281004, 7.59 Hs.420563, Hs.376818, Hs.15780, Hs.493450, Hs.306814, Hs.511810, Hs. 143241, Hs.278924, Hs.512632, Hs.367633, Hs.127363, Hs.414110, Hs.434143, Hs.36567, Hs.356605, Hs.414648, Hs.104888, Hs.197435, Hs.410810, Hs.434375, Hs.56279, Hs.125487, Hs.51011, Hs.170953, Hs.66392, Hs.48372, Hs.356664, Hs.420269, Hs.3017, Hs.306811, Hs.93121, Hs. 438880, Hs.302029, Hs.463017, Hs.412559, Hs.17518, Hs.2133, Hs.400380, Hs.75813, Hs.287656, Hs.334347, Hs.411572, Hs.6496, Hs.268764, Hs.385496, Hs.410088, Hs.24835, Hs.446504, Hs.7972, Hs.292057, Hs.435775, Hs.78106, Hs.217754, Hs.153704, Hs.341906, Hs.250830, Hs. 68846, Hs.438712, Hs.99480, Hs.434418, Hs.171980, Hs.376810, Hs.324335, Hs.37936, Hs.512546, Hs.128836, Hs.406330, Hs.302352, Hs.169825, Hs.522765, Hs.526526, Hs.82002, Hs.526394, Hs.51278 5, Hs.1937, Hs.105700, Hs.122895, Hs.9589, Hs.99439, Hs.334828, Hs.126161, Hs.465202, Hs.310231, Hs.94875, Hs.203557, Hs.444729, Hs.449965, Hs.122309, Hs.190090, Hs.355714, Hs.153952, Hs.408577, Hs.287370, Hs.185202, Hs.29792, Hs.4845, Hs.27183, Hs.486433, Hs. 439463, Hs.194665, Hs.129312, Hs.163682, Hs.73853, Hs.2689, Hs.283900, Hs.162604, Hs.381367, Hs.288218, Hs.203349, Hs.433891, Hs.248065, Hs.440603, Hs.144913, Hs.199316, Hs.221660, Hs.21554, Hs.510244, Hs.436325, Hs.117979, Hs.356888, Hs.306322, Hs.310431, Hs.356501, Hs. 444708, Hs.159609, Hs.18069, Hs.373612, Hs.12802, Hs.508901, Hs.504580, Hs.308242, Hs.128580, Hs.112184, Hs.306298, Hs.343800, Hs.50841, Hs.137516, Hs.413095, Hs.105640, Hs.144696, Hs.175411, Hs.207407, Hs.406856, Hs.122959, Hs.143792, Hs.170195, Hs.381715, Hs.443292, Hs. 56874, Hs.147096, Hs.521267, Hs.196956, Hs.80720, Hs.440953, Hs.352387, Hs.434107, Hs.112720, Hs.1555584, Hs.1473 52, Hs.146329, Hs.463171, Hs.445445, Hs.156892, Hs.212670, Hs.129895, Hs.356076, Hs.439590, Hs.278613, Hs.54697, Hs.388917, Hs.55923, Hs.460377, Hs.416244, Hs.234786, Hs.129969, Hs.275711, Hs.127842, Hs.302772, Hs.337008, Hs.48480, Hs.121115, Hs.318773, Hs.98564, Hs. 276466, Hs.78824, Hs.124502, Hs.325530, Hs.375664, Hs.516311, Hs.367688, Hs.386155, Hs.189174, Hs.159264, Hs.386143, Hs.158446, Hs.209461, Hs.163484, Hs.300559, Hs.155223, Hs.448979, Hs.368420, Hs.289044, Hs.74088, Hs.93907, Hs.408264, Hs.439168, Hs.437433, Hs.448468, Hs. 310194, Hs.45152, Hs.283095, Hs.500957, Hs.431156, Hs.511922, Hs.406549, Hs.288731, Hs.123419, Hs.334702, Hs.114172, Hs.408261, Hs.436657, Hs.125300, Hs.203, Hs.287499, Hs.102943, Hs.145949, Hs.248290, Hs.418167, Hs.55481, Hs.434181, Hs.275906, Hs.249126, Hs.147256, Hs. 178295, Hs.385890, Hs.201800, Hs.96633, Hs.445489, Hs.449650, Hs.102708, Hs.433381, Hs.27263, Hs.512782, H s.347537, Hs.124115, Hs.1270, Hs.349121, Hs.293884, Hs.143943, Hs.12742, Hs.166705, Hs.13209, Hs.77719, Hs.129694, Hs.126906, Hs. 151414, Hs.212584, Hs.232819, Hs.41324, Hs.140821, Hs.351593, Hs.12248, Hs.434281, Hs.283506, Hs.66412.18 Hs.2115872.17 Hs.427712.17 Hs.447703, Hs.372208 , Hs.379010, Hs.437008, Hs.99311, Hs.464896, Hs.3223079, Hs.509439, Hs.440088, Hs.34045, Hs.406073, Hs.455335, Hs.175465, Hs.507549, Hs .127775, Hs.132888, Hs.8728, Hs.157231, Hs.62863, Hs.169600, Hs.414332, Hs.259403, Hs.101949, Hs.436339, Hs.128668, Hs.380167, Hs.79340 , Hs.43125, Hs.2251, Hs.142296, Hs.288155, Hs.399740, Hs.27524, Hs.165464, Hs.123642, Hs.125156, Hs.58561, Hs.418966, Hs.447121, Hs .24734, Hs.348709, Hs.89436, Hs.2563, Hs.186363, Hs.169470, Hs.6540, Hs.223206, Hs.439169, Hs.133250, Hs.172562, Hs.349705, Hs.405457 , Hs.145894, Hs.7360, Hs.163546, Hs.102276, Hs.511781, Hs.283794, Hs.171090, Hs.508459, Hs.276808, Hs.2 48155, Hs.436042, Hs.371212, Hs.221040 and Hs.374990 any one or more of the genes, fragments thereof or complementary sequence chains thereof (21 ) Evaluation method of “certification” in Chinese medicine

(25) The above-mentioned target gene is the sequence number Hs.58323, Hs.160373, Hs.334832, Hs.150136, Hs.272385, Hs.382870, Hs.430919, Hs.387551, Hs.387551, Hs.194408 of the NCBI Unigene database. , Hs.406961, Hs.164021, Hs.24884, Hs.437211, Hs.436628, Hs.283880, Hs.110299, Hs.390242, Hs.113291, Hs.278894, Hs.90221, Hs.24583, Hs .266902, Hs.148956, Hs.162282, Hs.103388, Hs.406300, Hs.382347, Hs.413430, Hs.449884, Hs.24889, Hs.440606, Hs.36069, Hs.131255, Hs.369557 , Hs.512660, Hs.359981, Hs.283085, Hs.163546, Hs.117938, Hs.74376, Hs.438482, Hs.482392, Hs.320086, Hs.95990, Hs.9469, Hs.108642, Hs .180016, Hs.479572, Hs.169395, Hs.335319, Hs.442801, Hs.111726, Hs.183390, Hs.449407, Hs.434195, Hs.17546, Hs.127951, Hs.458408, Hs.287374 , Hs.150826, Hs.181483, Hs.115467, Hs.232779, Hs.96557, Hs.406722, Hs.201269, Hs.47313, Hs.8852, Hs.283326, Hs.225943, Hs.12902, Hs .500371, Hs.7296, Hs.430854, Hs.278568, Hs.13227 5, Hs.66392, Hs.437179, Hs.8850, Hs.98967, Hs.57876, Hs.459828, Hs.310230, Hs.25328, Hs.301956, Hs.144875, Hs.110457, Hs.509265, Hs.60339, Hs.511880, Hs.446152, Hs.48100, Hs.247975, Hs.479080, Hs.490096, Hs.8346, Hs.131555, Hs.32967, Hs.307003, Hs.127032, Hs. 109268, Hs.80720, Hs.266738, Hs.113912, Hs.175780, Hs.497972, Hs.18213, Hs.152664, Hs.459847, Hs.378736, Hs.458427, Hs.36761, Hs.429531, Hs.149797, Hs.444135, Hs.22575, Hs.272191, Hs.512643, Hs.54713, Hs.820, Hs.77221, Hs.253690, Hs.436282, Hs.181289, Hs.170560, Hs. 426734, Hs.334527, Hs.406701, Hs.177434, Hs.511968, Hs.389964, Hs.251699, Hs.207805, Hs.140821, Hs.281004, Hs.435675, Hs.292597, Hs.178715, Hs.161394, Hs.396332, Hs.302109, Hs.86088, Hs.356794, Hs.449392, Hs.134807, Hs.210850, Hs.18705, Hs.326444, Hs.287453, Hs.130522, Hs. 234775, Hs.438555, Hs.171532, Hs.54617, Hs.268843, Hs.11713, Hs.5025, Hs.288727, Hs.412022, Hs.78465, Hs.423112, Hs. 497707, Hs.235390, Hs.33455, Hs.371278, Hs.237642, Hs.234790, Hs.78792, Hs.115181, Hs.115617, Hs.463017, Hs.406789, Hs.378753, Hs.256583, Hs.99430, Hs.525361, Hs.148559, Hs.249181, Hs.23853, Hs.172052, Hs.90821, Hs.444182, Hs.123474, Hs.284310, Hs.137574, Hs.102929, Hs. 511985, Hs.371335, Hs.528103, Hs.355711, Hs.496614, Hs.27621, Hs.183006, Hs.27021, Hs.389571, Hs.227457, Hs.334772, Hs.512760, Hs.53996, Hs.207407, Hs.297997, Hs.512808, Hs.149467, Hs.510932, Hs.272493, Hs.79881, Hs.3052, Hs.415895, Hs.388220, Hs.253193, Hs.124502, Hs. 232803, Hs.269777, Hs.12264, Hs.380763, Hs.147595, Hs.411274, Hs.441481, Hs.376025, Hs.250895, Hs.468922, Hs.161427, Hs.385986, Hs.439760, Hs.313413, Hs.408843, Hs.58974, Hs.430755, Hs.99120, Hs.25363, Hs.287405, Hs.131035, Hs.335355, Hs.259471, Hs.235888, Hs.144527, Hs. 414091, Hs.480174, Hs.445241, Hs.44888, Hs.382683, Hs.439494, Hs.288520, Hs.234027, Hs.130685, Hs.399966 Hs.434486, Hs.407002, Hs.333958, Hs.238648, Hs.308045, Hs.196470, Hs.144531, Hs.7327, Hs.83916, Hs.169441, Hs.73890, Hs.432374, Hs. 143751, Hs.2074, Hs.158278, Hs.158963, Hs.490216, Hs.103422, Hs.133361, Hs.404220, Hs.169071, Hs.288718, Hs.144130, Hs.336224, Hs.112669, Hs.159265, Hs.122110, Hs.7835, Hs.407015, Hs.441708, Hs.452398, Hs.439124, Hs.443020, Hs.499520, Hs.356572, Hs.284200, Hs.78944, Hs. 512387, Hs.235498, Hs.133082, Hs.471423, Hs.73625, Hs.9914, Hs.355862, Hs.82120, Hs.443392, Hs.401954, Hs.436140, Hs.461482, Hs.433596, Hs.434103, Hs.385898, Hs.348920, Hs.81848, Hs.432379, Hs.79025, Hs.407162, Hs.197922, Hs.180171, Hs.408200, Hs.444529, Hs.164649, Hs. 4224, Hs.460471, Hs.149152, Hs.458397, Hs.124211, Hs.415497, Hs.293917, Hs.253489, Hs.14822, Hs.376590, Hs.179112, Hs.152337, Hs.438300, Hs.344099, Hs.421986, Hs.432458, Hs.351414, Hs.54460, Hs.349227, Hs.47312, Hs.185796, Hs.26981, Hs.267038, Hs.448749, Hs.170114, Hs.95582, Hs.352601, Hs.353001, Hs.352617, Hs.414164, Hs.30340, Hs.359978, Hs.298241, Hs.178292, Hs.156232, Hs. 406681, Hs.411644, Hs.97933, Hs.137674, Hs.476396, Hs.388877, Hs.155573, Hs.87, Hs.153203, Hs.458434, Hs.10056, Hs.148074 and Hs.377022 Any one or more of the described genes, a fragment thereof, or a complementary sequence chain thereof, The evaluation method of “proof” in Chinese medicine according to the above (21);

(26) The above target genes are represented by SEQ ID NOs: Hs.436450, Hs.278613, Hs.411239, Hs.445072, Hs.123515, Hs.17518, Hs.128668, Hs.280226, Hs.511758 in the NCBI Unigene database , Hs.233437, Hs.187802, Hs.61929, Hs.406741, Hs.245431, Hs.20082, Hs.29798, Hs.222, Hs.162089, Hs.37936, Hs.144913, Hs.166351, Hs .313, Hs.522901, Hs.450230, Hs.89679, Hs.323966, Hs.439168, Hs.159629, Hs.186363, Hs.172052, Hs.250723, Hs.356605, Hs.376407, Hs.188021 , Hs.386264, Hs.40510, Hs.408067, Hs.58009, Hs.222909, Hs.113227, Hs.350800, Hs.377070, Hs.178295, Hs.222746, Hs.351811, Hs.440088, Hs .490397, Hs.344400, Hs.361748, Hs.283045, Hs.27258, Hs.25328, Hs.350765, Hs.103665, Hs.194725, Hs.327621, Hs.408629, Hs.411347, Hs.489592 , Hs.386236, Hs.446451, Hs.105115, Hs.45207, Hs.91283, Hs.178499, Hs.444882, Hs.381370, Hs.369994, Hs.93883, Hs.306230, Hs.413924, Hs .504809, Hs.84665, Hs.389724, Hs.211587, Hs.1560, Hs.406733, Hs.306812, Hs.348745, Hs.106688, Hs.129895, Hs.388917, Hs.368746, Hs.413525, Hs.166011, Hs.94865, Hs.250472, Hs.434485, Hs. 267038, Hs.445426, Hs.154084, Hs.23317, Hs.75360, Hs.127405, Hs.155584, Hs.47504, Hs.90318, Hs.128499, Hs.283082, Hs.449006, Hs.309365, Hs.436602, Hs.324335, Hs.351816, Hs.306992, Hs.512632, Hs.443260, Hs.348709, Hs.426324, Hs.227656, Hs.435092, Hs.272200, Hs.347537, Hs. 271269, Hs.424414, Hs.707, Hs.248204, Hs.168355, Hs.58559, Hs.407817, Hs.447162, Hs.144870, Hs.435398, Hs.146025, Hs.406485, Hs.326559, Hs.258536, Hs.169825, Hs.1270, Hs.325335, Hs.335877, Hs.421280, Hs.443120, Hs.389638, Hs.124673, Hs.150138, Hs.476618, Hs.438482, Hs. 652, Hs.199372, Hs.493952, Hs.371212, Hs.25156, Hs.301956, Hs.511810, Hs.151408, Hs.157144, Hs.159525, Hs.94327, Hs.449791, Hs.320522, Hs.123114, Hs.446376, Hs.250666, Hs.201554, Hs.351818, Hs.189575, Hs.132270, Hs.36793, Hs.23367, Hs.104613 Hs.254006, Hs.133995, Hs.395205, Hs.476835, Hs.513009, Hs.257584, Hs.5462, Hs.96149, Hs.374720, Hs.46791, Hs.127473, Hs.91813, Hs. 271640, Hs.379010, Hs.516739, Hs.376657, Hs.121490, Hs.352240, Hs.203557, Hs.228982, Hs.122115, Hs.112928, Hs.436975, Hs.387258, Hs.433197, Hs.159553, Hs.437, Hs.288911, Hs.440953, Hs.20315, Hs.104859, Hs.38260, Hs.199316, Hs.511791, Hs.282275, Hs.249126, Hs.163484, Hs. 297604, Hs.73853, Hs.27335, Hs.26176, Hs.92683, Hs.212584, Hs.372616, Hs.153647, Hs.121115, Hs.87306, Hs.318775, Hs.353114, Hs.167679, Hs.415209, Hs.55209, Hs.143600, Hs.156007, Hs.444960, Hs.234016, Hs.283011, Hs.512778, Hs.37107, Hs.414332, Hs.164680, Hs.74115, Hs. 422120, Hs.104133, Hs.21861, Hs.249184, Hs.375054, Hs.436424, Hs.302754, Hs.297452, Hs.165387, Hs.493450, Hs.306094, Hs.414110, Hs.118057, Hs.840, Hs.2207, Hs.343154, Hs.445772, Hs.132272, Hs.59892, Hs.380519, Hs.442936, Hs.274548, Hs.72910, Hs.4 9433, Hs.26663, Hs.155814, Hs.212892, Hs.408488, Hs.37125, Hs.375870, Hs.440695, Hs.341906, Hs.370622, Hs.314141, Hs.118631, Hs.115365, Hs.238964, Hs.331491, Hs.194714, Hs.434375, Hs.127012, Hs.306622, Hs.211298, Hs.264330, Hs.347534, Hs.148767, Hs.41270, Hs.283096, Hs. 110637, Hs.76152, Hs.334, Hs.55412, Hs.497743, Hs.276808, Hs.114599, Hs.461085, Hs.282787, Hs.131834, Hs.21838, Hs.492218, Hs.248114, Hs.501321, Hs.512787, Hs.268700, Hs.313948, Hs.211477, Hs.2053, Hs.21388, Hs.507546, Hs.1501, Hs.248139, Hs.283779, Hs.511992, Hs. 406984, Hs.145680, Hs.403836, Hs.287656, Hs.53263, Hs.257541, Hs.413265, Hs.333742, Hs.156346, Hs.441287, Hs.131101, Hs.436983, Hs.256036, Hs.420036, Hs.440387, Hs.122309, Hs.479817, Hs.195614, Hs.355584, Hs.65248, Hs.512782, Hs.363315, Hs.43107, Hs.233950, Hs.445445, Hs. Any one or more of the genes described in 82316, Hs.148990, Hs.158798, Hs.189067 and Hs.90797 Evaluation method for "witness" of a gene, in Chinese medicine according to (21), which is a fragment or complementary sequence strand thereof;

(27) The above target gene is characterized in that the expression of the target gene varies depending on the appearance and disappearance of “Senka Koboku no proof” and / or the ease of use of Hanka Koboku hot water. ) Evaluation method of “certification” in Chinese medicine

(28) The target gene is any one or more of genes including a base sequence described in SEQ ID NO: 290 to SEQ ID NO: 338 of the sequence listing or a part thereof, a fragment thereof, or a complementary sequence chain thereof. The evaluation method of “certification” in Chinese medicine according to (27) above,

  (29) Kampo using the gene according to any one of (1), (2), (3), (6) and (10) above, a fragment thereof or a complementary sequence chain thereof as a target gene A method for performing an adaptive diagnosis of Kampo medicines in medicine, measuring the expression state of a target gene in a measurement sample obtained from a subject, and measuring the measurement result as the expression state of the target gene in a comparative sample serving as an evaluation standard. A method for adaptive diagnosis of Kampo medicine in Kampo medicine, wherein the subject is observed for the presence or absence of a gene expression pattern peculiar to Kampo medicine by comparing and evaluating and judging the health condition of the subject;

(30) The above-mentioned Chinese herbal medicine is Kosyosan, and the target gene changes with the appearance / disappearance of “proof of Kosyosan”, the use of Kasosan and / or the effectiveness of a specific Chinese medicine The method for adaptive diagnosis of Chinese medicine in Chinese medicine according to (29) above,

(31) The target gene is a gene containing the base sequence described in SEQ ID NO: 1 to SEQ ID NO: 49 in the sequence listing or a part thereof, and the sequence numbers Hs.487001, Hs.436042 and Hs.61714 in NCBI Unigene database. Any one or more of the genes described in 1), a fragment thereof, or a complementary sequence chain thereof;

(32) The above target genes are represented by the sequence numbers Hs.180911, Hs.294176, Hs.53250, Hs.282331, Hs.440606, Hs.86638, Hs.61232, Hs.24884, Hs.110299 in the database of NCBI Unigene. , Hs.303523, Hs.115617, Hs.3321, Hs.334527, Hs.161640, Hs.225943, Hs.323213, Hs.186582, Hs.24583, Hs.234775, Hs.512633, Hs.377022, Hs .29189, Hs.262480, Hs.8852, Hs.527699, Hs.445241, Hs.437457, Hs.436628, Hs.115497, Hs.497707, Hs.150826, Hs.266902, Hs.442801, Hs.8022 , Hs.432379, Hs.460471, Hs.434195, Hs.145156, Hs.396566, Hs.367761, Hs.12902, Hs.297997, Hs.183390, Hs.479572, Hs.450425, Hs.283085, Hs .103388, Hs.18213, Hs.378736, Hs.389964, Hs.387258, Hs.355820, Hs.210850, Hs.158318, Hs.157461, Hs.17860, Hs.355711, Hs.74376, Hs.111726 , Hs.54468, Hs.75236, Hs.193228, Hs.512660, Hs.130685, Hs.272493, Hs.414091, Hs.113912, Hs.128204, Hs.115181, Hs.86088, Hs.431156, Hs .115830, Hs.37058, Hs.125507, Hs.376586, Hs.97808 Hs.493514, Hs.412355, Hs.97933, Hs.288727, Hs.499520, Hs.53996, Hs.144028, Hs.131555, Hs.232779, Hs.382347, Hs.382224, Hs.382870, Hs. 82535, Hs.235498, Hs.438482, Hs.129778, Hs.22575, Hs.98967, Hs.320086, Hs.412022, Hs.259498, Hs.445025, Hs.334022, Hs.51120, Hs.302109, Hs.268744, Hs.152664, Hs.153203, Hs.201269, Hs.448749, Hs.408843, Hs.234790, Hs.147305, Hs.436494, Hs.12840, Hs.158963, Hs.283880, Hs. 476396, Hs.148956, Hs.326012, Hs.437137, Hs.406763, Hs.150276, Hs.408195, Hs.496614, Hs.251573, Hs.205457, Hs.132275, Hs.233325, Hs.343666, Hs.49753, Hs.432815, Hs.443683, Hs.143907, Hs.22116, Hs.336224, Hs.22573, Hs.66, Hs.234774, Hs.130310, Hs.25363, Hs.78465, Hs. 367888, Hs.127059, Hs.140821, Hs.511968, Hs.432445, Hs.350765, Hs.48695, Hs.430138, Hs.99607, Hs.213493, Hs.8345, Hs.502577, Hs.57876, Hs.100299, Hs.458408, Hs.406639, Hs.512760, Hs.123457, Hs.326561, Hs.446702, Hs.89121, Hs.17681, Hs.148074, Hs. 97714, Hs.2936, Hs.158357, Hs.499247, Hs.153692, Hs.406234, Hs.241385, Hs.271809, Hs.278568, Hs.254122, Hs.318791, Hs.189482, Hs.282938, Hs.390808, Hs.2149, Hs.423112, Hs.301715, Hs.55016, Hs.99120, Hs.511985, Hs.359978, Hs.204238, Hs.283506, Hs.447877, Hs.137674, Hs. 266738, Hs.302145, Hs.399966, Hs.149098, Hs.431010, Hs.222312, Hs.2207, Hs.25094, Hs.79881, Hs.116651, Hs.25328, Hs.501480, Hs.8346, Hs.157796, Hs.21239, Hs.508901, Hs.144875, Hs.40735, Hs.341806, Hs.125423, Hs.511880, Hs.433827, Hs.307044, Hs.306697, Hs.134807, Hs. 387367, Hs.335355, Hs.349109, Hs.418105, Hs.376337, Hs.137574, Hs.44077, Hs.44899, Hs.184945, Hs.114088, Hs.376025, Hs.21258, Hs.207407, Hs.117938, Hs.169071, Hs.385986, Hs.284153, Hs.95582, Hs.21380, Hs.418083, Hs.91625, Hs.397167, Hs.437046, Hs.14468, Hs.26309, Hs. 479080, Hs.352530, Hs.73958, Hs.171025, Hs.121001, Hs.11713, Hs.280311, Hs.8535, Hs.283326, Hs.407474, Hs.451697 Hs.14732, Hs.73890, Hs.42650, Hs.7327, Hs.287640, Hs.194659, Hs.191381, Hs.406789, Hs.227098, Hs.7934, Hs.512643, Hs.256583, Hs. 408355, Hs.155182, Hs.30340, Hs.178023, Hs.58974, Hs.390568, Hs.185796, Hs.115263, Hs.414251, Hs.119693, Hs.511776, Hs.196530, Hs.157573, Hs.36761, Hs.223581, Hs.511753, Hs.17170, Hs.448823, Hs.102929, Hs.37616, Hs.511824, Hs.354013, Hs.132380, Hs.371496, Hs.458427, Hs. 32967, Hs.143751, Hs.253690, Hs.352, Hs.21177, Hs.406681, Hs.435976, Hs.46679, Hs.148101, Hs.9914, Hs.310230, Hs.376590, Hs.458397, Hs.58414, Hs.17165, Hs.407015, Hs.470704, Hs.246914, Hs.15420, Hs.436282, Hs.257368, Hs.512739, Hs.387679, Hs.283078, Hs.516782, Hs. 109752, Hs.201667, Hs.149504, Hs.398139, Hs.94367, Hs.112208, Hs.395779, Hs.445563, Hs.65135, Hs.187203, Hs.490216, Hs.487918, Hs.436495, Hs.125571, Hs.519634, Hs.399769, Hs.511987, Hs.26176, Hs.25934, Hs.351825, Hs.283794, Hs.256067, Hs.251699, Hs.436 378, Hs.523755, Hs.449884, Hs.41154, Hs.54609, Hs.458291, Hs.408336 and Hs.2012 One or more genes, fragments thereof or complements thereof An adaptive diagnosis method for Kampo medicine in Kampo medicine according to (30) above, which is a sequence chain;

(33) The above target genes are represented by NCBI Unigene database SEQ ID NOs: Hs.372133, Hs.281004, 7.59 Hs.420563, Hs.376818, Hs.15780, Hs.493450, Hs.306814, Hs.511810, Hs. 143241, Hs.278924, Hs.512632, Hs.367633, Hs.127363, Hs.414110, Hs.434143, Hs.36567, Hs.356605, Hs.414648, Hs.104888, Hs.197435, Hs.410810, Hs.434375, Hs.56279, Hs.125487, Hs.51011, Hs.170953, Hs.66392, Hs.48372, Hs.356664, Hs.420269, Hs.3017, Hs.306811, Hs.93121, Hs. 438880, Hs.302029, Hs.463017, Hs.412559, Hs.17518, Hs.2133, Hs.400380, Hs.75813, Hs.287656, Hs.334347, Hs.411572, Hs.6496, Hs.268764, Hs.385496, Hs.410088, Hs.24835, Hs.446504, Hs.7972, Hs.292057, Hs.435775, Hs.78106, Hs.217754, Hs.153704, Hs.341906, Hs.250830, Hs. 68846, Hs.438712, Hs.99480, Hs.434418, Hs.171980, Hs.376810, Hs.324335, Hs.37936, Hs.512546, Hs.128836, Hs.406330, Hs.302352, Hs.169825, Hs.522765, Hs.526526, Hs.82002, Hs.526394, Hs.51278 5, Hs.1937, Hs.105700, Hs.122895, Hs.9589, Hs.99439, Hs.334828, Hs.126161, Hs.465202, Hs.310231, Hs.94875, Hs.203557, Hs.444729, Hs.449965, Hs.122309, Hs.190090, Hs.355714, Hs.153952, Hs.408577, Hs.287370, Hs.185202, Hs.29792, Hs.4845, Hs.27183, Hs.486433, Hs. 439463, Hs.194665, Hs.129312, Hs.163682, Hs.73853, Hs.2689, Hs.283900, Hs.162604, Hs.381367, Hs.288218, Hs.203349, Hs.433891, Hs.248065, Hs.440603, Hs.144913, Hs.199316, Hs.221660, Hs.21554, Hs.510244, Hs.436325, Hs.117979, Hs.356888, Hs.306322, Hs.310431, Hs.356501, Hs. 444708, Hs.159609, Hs.18069, Hs.373612, Hs.12802, Hs.508901, Hs.504580, Hs.308242, Hs.128580, Hs.112184, Hs.306298, Hs.343800, Hs.50841, Hs.137516, Hs.413095, Hs.105640, Hs.144696, Hs.175411, Hs.207407, Hs.406856, Hs.122959, Hs.143792, Hs.170195, Hs.381715, Hs.443292, Hs. 56874, Hs.147096, Hs.521267, Hs.196956, Hs.80720, Hs.440953, Hs.352387, Hs.434107, Hs.112720, Hs.1555584, Hs.1473 52, Hs.146329, Hs.463171, Hs.445445, Hs.156892, Hs.212670, Hs.129895, Hs.356076, Hs.439590, Hs.278613, Hs.54697, Hs.388917, Hs.55923, Hs.460377, Hs.416244, Hs.234786, Hs.129969, Hs.275711, Hs.127842, Hs.302772, Hs.337008, Hs.48480, Hs.121115, Hs.318773, Hs.98564, Hs. 276466, Hs.78824, Hs.124502, Hs.325530, Hs.375664, Hs.516311, Hs.367688, Hs.386155, Hs.189174, Hs.159264, Hs.386143, Hs.158446, Hs.209461, Hs.163484, Hs.300559, Hs.155223, Hs.448979, Hs.368420, Hs.289044, Hs.74088, Hs.93907, Hs.408264, Hs.439168, Hs.437433, Hs.448468, Hs. 310194, Hs.45152, Hs.283095, Hs.500957, Hs.431156, Hs.511922, Hs.406549, Hs.288731, Hs.123419, Hs.334702, Hs.114172, Hs.408261, Hs.436657, Hs.125300, Hs.203, Hs.287499, Hs.102943, Hs.145949, Hs.248290, Hs.418167, Hs.55481, Hs.434181, Hs.275906, Hs.249126, Hs.147256, Hs. 178295, Hs.385890, Hs.201800, Hs.96633, Hs.445489, Hs.449650, Hs.102708, Hs.433381, Hs.27263, Hs.512782, H s.347537, Hs.124115, Hs.1270, Hs.349121, Hs.293884, Hs.143943, Hs.12742, Hs.166705, Hs.13209, Hs.77719, Hs.129694, Hs.126906, Hs. 151414, Hs.212584, Hs.232819, Hs.41324, Hs.140821, Hs.351593, Hs.12248, Hs.434281, Hs.283506, Hs.66412.18 Hs.2115872.17 Hs.427712.17 Hs.447703, Hs.372208 , Hs.379010, Hs.437008, Hs.99311, Hs.464896, Hs.3223079, Hs.509439, Hs.440088, Hs.34045, Hs.406073, Hs.455335, Hs.175465, Hs.507549, Hs .127775, Hs.132888, Hs.8728, Hs.157231, Hs.62863, Hs.169600, Hs.414332, Hs.259403, Hs.101949, Hs.436339, Hs.128668, Hs.380167, Hs.79340 , Hs.43125, Hs.2251, Hs.142296, Hs.288155, Hs.399740, Hs.27524, Hs.165464, Hs.123642, Hs.125156, Hs.58561, Hs.418966, Hs.447121, Hs .24734, Hs.348709, Hs.89436, Hs.2563, Hs.186363, Hs.169470, Hs.6540, Hs.223206, Hs.439169, Hs.133250, Hs.172562, Hs.349705, Hs.405457 , Hs.145894, Hs.7360, Hs.163546, Hs.102276, Hs.511781, Hs.283794, Hs.171090, Hs.508459, Hs.276808, Hs.2 48155, Hs.436042, Hs.371212, Hs.221040 and Hs.374990 any one or more of the genes, fragments thereof or complementary sequence chains thereof (30 ) The method of adaptive diagnosis of Chinese medicine in Chinese medicine;

(34) The above-mentioned target gene is SEQ ID NO: Hs.58323, Hs.160373, Hs.334832, Hs.150136, Hs.272385, Hs.382870, Hs.430919, Hs.387551, Hs.387551, and Hs.194408 in the NCBI Unigene database. , Hs.406961, Hs.164021, Hs.24884, Hs.437211, Hs.436628, Hs.283880, Hs.110299, Hs.390242, Hs.113291, Hs.278894, Hs.90221, Hs.24583, Hs .266902, Hs.148956, Hs.162282, Hs.103388, Hs.406300, Hs.382347, Hs.413430, Hs.449884, Hs.24889, Hs.440606, Hs.36069, Hs.131255, Hs.369557 , Hs.512660, Hs.359981, Hs.283085, Hs.163546, Hs.117938, Hs.74376, Hs.438482, Hs.482392, Hs.320086, Hs.95990, Hs.9469, Hs.108642, Hs .180016, Hs.479572, Hs.169395, Hs.335319, Hs.442801, Hs.111726, Hs.183390, Hs.449407, Hs.434195, Hs.17546, Hs.127951, Hs.458408, Hs.287374 , Hs.150826, Hs.181483, Hs.115467, Hs.232779, Hs.96557, Hs.406722, Hs.201269, Hs.47313, Hs.8852, Hs.283326, Hs.225943, Hs.12902, Hs .500371, Hs.7296, Hs.430854, Hs.278568, Hs.13227 5, Hs.66392, Hs.437179, Hs.8850, Hs.98967, Hs.57876, Hs.459828, Hs.310230, Hs.25328, Hs.301956, Hs.144875, Hs.110457, Hs.509265, Hs.60339, Hs.511880, Hs.446152, Hs.48100, Hs.247975, Hs.479080, Hs.490096, Hs.8346, Hs.131555, Hs.32967, Hs.307003, Hs.127032, Hs. 109268, Hs.80720, Hs.266738, Hs.113912, Hs.175780, Hs.497972, Hs.18213, Hs.152664, Hs.459847, Hs.378736, Hs.458427, Hs.36761, Hs.429531, Hs.149797, Hs.444135, Hs.22575, Hs.272191, Hs.512643, Hs.54713, Hs.820, Hs.77221, Hs.253690, Hs.436282, Hs.181289, Hs.170560, Hs. 426734, Hs.334527, Hs.406701, Hs.177434, Hs.511968, Hs.389964, Hs.251699, Hs.207805, Hs.140821, Hs.281004, Hs.435675, Hs.292597, Hs.178715, Hs.161394, Hs.396332, Hs.302109, Hs.86088, Hs.356794, Hs.449392, Hs.134807, Hs.210850, Hs.18705, Hs.326444, Hs.287453, Hs.130522, Hs. 234775, Hs.438555, Hs.171532, Hs.54617, Hs.268843, Hs.11713, Hs.5025, Hs.288727, Hs.412022, Hs.78465, Hs.423112, Hs. 497707, Hs.235390, Hs.33455, Hs.371278, Hs.237642, Hs.234790, Hs.78792, Hs.115181, Hs.115617, Hs.463017, Hs.406789, Hs.378753, Hs.256583, Hs.99430, Hs.525361, Hs.148559, Hs.249181, Hs.23853, Hs.172052, Hs.90821, Hs.444182, Hs.123474, Hs.284310, Hs.137574, Hs.102929, Hs. 511985, Hs.371335, Hs.528103, Hs.355711, Hs.496614, Hs.27621, Hs.183006, Hs.27021, Hs.389571, Hs.227457, Hs.334772, Hs.512760, Hs.53996, Hs.207407, Hs.297997, Hs.512808, Hs.149467, Hs.510932, Hs.272493, Hs.79881, Hs.3052, Hs.415895, Hs.388220, Hs.253193, Hs.124502, Hs. 232803, Hs.269777, Hs.12264, Hs.380763, Hs.147595, Hs.411274, Hs.441481, Hs.376025, Hs.250895, Hs.468922, Hs.161427, Hs.385986, Hs.439760, Hs.313413, Hs.408843, Hs.58974, Hs.430755, Hs.99120, Hs.25363, Hs.287405, Hs.131035, Hs.335355, Hs.259471, Hs.235888, Hs.144527, Hs. 414091, Hs.480174, Hs.445241, Hs.44888, Hs.382683, Hs.439494, Hs.288520, Hs.234027, Hs.130685, Hs.399966 Hs.434486, Hs.407002, Hs.333958, Hs.238648, Hs.308045, Hs.196470, Hs.144531, Hs.7327, Hs.83916, Hs.169441, Hs.73890, Hs.432374, Hs. 143751, Hs.2074, Hs.158278, Hs.158963, Hs.490216, Hs.103422, Hs.133361, Hs.404220, Hs.169071, Hs.288718, Hs.144130, Hs.336224, Hs.112669, Hs.159265, Hs.122110, Hs.7835, Hs.407015, Hs.441708, Hs.452398, Hs.439124, Hs.443020, Hs.499520, Hs.356572, Hs.284200, Hs.78944, Hs. 512387, Hs.235498, Hs.133082, Hs.471423, Hs.73625, Hs.9914, Hs.355862, Hs.82120, Hs.443392, Hs.401954, Hs.436140, Hs.461482, Hs.433596, Hs.434103, Hs.385898, Hs.348920, Hs.81848, Hs.432379, Hs.79025, Hs.407162, Hs.197922, Hs.180171, Hs.408200, Hs.444529, Hs.164649, Hs. 4224, Hs.460471, Hs.149152, Hs.458397, Hs.124211, Hs.415497, Hs.293917, Hs.253489, Hs.14822, Hs.376590, Hs.179112, Hs.152337, Hs.438300, Hs.344099, Hs.421986, Hs.432458, Hs.351414, Hs.54460, Hs.349227, Hs.47312, Hs.185796, Hs.26981, Hs.267038, Hs.448749, Hs.170114, Hs.95582, Hs.352601, Hs.353001, Hs.352617, Hs.414164, Hs.30340, Hs.359978, Hs.298241, Hs.178292, Hs.156232, Hs. 406681, Hs.411644, Hs.97933, Hs.137674, Hs.476396, Hs.388877, Hs.155573, Hs.87, Hs.153203, Hs.458434, Hs.10056, Hs.148074 and Hs.377022 Any one or more of the described genes, a fragment thereof, or a complementary sequence chain thereof, The method for adaptive diagnosis of Kampo medicine in Kampo medicine according to (30) above;

(35) The above-mentioned target genes are represented by the sequence numbers Hs. 436450, Hs. 278613, Hs. 411239, Hs. 445072, Hs. 123515, Hs. 17518, Hs. 128668, Hs. 280226, Hs. 511758 of NCBI Unigene database. , Hs.233437, Hs.187802, Hs.61929, Hs.406741, Hs.245431, Hs.20082, Hs.29798, Hs.222, Hs.162089, Hs.37936, Hs.144913, Hs.166351, Hs .313, Hs.522901, Hs.450230, Hs.89679, Hs.323966, Hs.439168, Hs.159629, Hs.186363, Hs.172052, Hs.250723, Hs.356605, Hs.376407, Hs.188021 , Hs.386264, Hs.40510, Hs.408067, Hs.58009, Hs.222909, Hs.113227, Hs.350800, Hs.377070, Hs.178295, Hs.222746, Hs.351811, Hs.440088, Hs .490397, Hs.344400, Hs.361748, Hs.283045, Hs.27258, Hs.25328, Hs.350765, Hs.103665, Hs.194725, Hs.327621, Hs.408629, Hs.411347, Hs.489592 , Hs.386236, Hs.446451, Hs.105115, Hs.45207, Hs.91283, Hs.178499, Hs.444882, Hs.381370, Hs.369994, Hs.93883, Hs.306230, Hs.413924, Hs .504809, Hs.84665, Hs.389724, Hs.211587, Hs.1560, Hs.406733, Hs.306812, Hs.348745, Hs.106688, Hs.129895, Hs.388917, Hs.368746, Hs.413525, Hs.166011, Hs.94865, Hs.250472, Hs.434485, Hs. 267038, Hs.445426, Hs.154084, Hs.23317, Hs.75360, Hs.127405, Hs.155584, Hs.47504, Hs.90318, Hs.128499, Hs.283082, Hs.449006, Hs.309365, Hs.436602, Hs.324335, Hs.351816, Hs.306992, Hs.512632, Hs.443260, Hs.348709, Hs.426324, Hs.227656, Hs.435092, Hs.272200, Hs.347537, Hs. 271269, Hs.424414, Hs.707, Hs.248204, Hs.168355, Hs.58559, Hs.407817, Hs.447162, Hs.144870, Hs.435398, Hs.146025, Hs.406485, Hs.326559, Hs.258536, Hs.169825, Hs.1270, Hs.325335, Hs.335877, Hs.421280, Hs.443120, Hs.389638, Hs.124673, Hs.150138, Hs.476618, Hs.438482, Hs. 652, Hs.199372, Hs.493952, Hs.371212, Hs.25156, Hs.301956, Hs.511810, Hs.151408, Hs.157144, Hs.159525, Hs.94327, Hs.449791, Hs.320522, Hs.123114, Hs.446376, Hs.250666, Hs.201554, Hs.351818, Hs.189575, Hs.132270, Hs.36793, Hs.23367, Hs.104613 Hs.254006, Hs.133995, Hs.395205, Hs.476835, Hs.513009, Hs.257584, Hs.5462, Hs.96149, Hs.374720, Hs.46791, Hs.127473, Hs.91813, Hs. 271640, Hs.379010, Hs.516739, Hs.376657, Hs.121490, Hs.352240, Hs.203557, Hs.228982, Hs.122115, Hs.112928, Hs.436975, Hs.387258, Hs.433197, Hs.159553, Hs.437, Hs.288911, Hs.440953, Hs.20315, Hs.104859, Hs.38260, Hs.199316, Hs.511791, Hs.282275, Hs.249126, Hs.163484, Hs. 297604, Hs.73853, Hs.27335, Hs.26176, Hs.92683, Hs.212584, Hs.372616, Hs.153647, Hs.121115, Hs.87306, Hs.318775, Hs.353114, Hs.167679, Hs.415209, Hs.55209, Hs.143600, Hs.156007, Hs.444960, Hs.234016, Hs.283011, Hs.512778, Hs.37107, Hs.414332, Hs.164680, Hs.74115, Hs. 422120, Hs.104133, Hs.21861, Hs.249184, Hs.375054, Hs.436424, Hs.302754, Hs.297452, Hs.165387, Hs.493450, Hs.306094, Hs.414110, Hs.118057, Hs.840, Hs.2207, Hs.343154, Hs.445772, Hs.132272, Hs.59892, Hs.380519, Hs.442936, Hs.274548, Hs.72910, Hs.4 9433, Hs.26663, Hs.155814, Hs.212892, Hs.408488, Hs.37125, Hs.375870, Hs.440695, Hs.341906, Hs.370622, Hs.314141, Hs.118631, Hs.115365, Hs.238964, Hs.331491, Hs.194714, Hs.434375, Hs.127012, Hs.306622, Hs.211298, Hs.264330, Hs.347534, Hs.148767, Hs.41270, Hs.283096, Hs. 110637, Hs.76152, Hs.334, Hs.55412, Hs.497743, Hs.276808, Hs.114599, Hs.461085, Hs.282787, Hs.131834, Hs.21838, Hs.492218, Hs.248114, Hs.501321, Hs.512787, Hs.268700, Hs.313948, Hs.211477, Hs.2053, Hs.21388, Hs.507546, Hs.1501, Hs.248139, Hs.283779, Hs.511992, Hs. 406984, Hs.145680, Hs.403836, Hs.287656, Hs.53263, Hs.257541, Hs.413265, Hs.333742, Hs.156346, Hs.441287, Hs.131101, Hs.436983, Hs.256036, Hs.420036, Hs.440387, Hs.122309, Hs.479817, Hs.195614, Hs.355584, Hs.65248, Hs.512782, Hs.363315, Hs.43107, Hs.233950, Hs.445445, Hs. Any one or more of the genes described in 82316, Hs.148990, Hs.158798, Hs.189067 and Hs.90797 The method of genetic adaptation diagnosis of Chinese medicine in Chinese medicine according to (30), which is a fragment or complementary sequence strand thereof;

(36) The Chinese herbal medicine is Hanka-koboku-to, and the target gene changes with the appearance and disappearance of the “proof of Hanka-kobaku-to” and / or the effect of Hanka-koboku-to by the effectiveness of Hanka-koboku-to. The method of adaptive diagnosis of Chinese medicine in Chinese medicine according to (29) above, and

(37) The target gene is any one or more of the base sequences described in SEQ ID NO: 290 to SEQ ID NO: 338 of the sequence listing or a part thereof, a fragment thereof, or a complementary sequence chain thereof The method for adaptive diagnosis of Chinese medicine in Chinese medicine according to (36) above,

  These genes used for the diagnosis of Chinese medicine according to the present invention and techniques for using them were born as a result of various repeated studies by the present inventors. That is, the present inventor has adopted the idea of EBM (Evidence-Based Medicine), which has become a global trend in the medical field in recent years, as well as the Western medical field. In order to put it into practice in this field, we have been diligently engaged in various research studies focusing on the effective use of human genome sequence information.

  In other words, the present inventor has no accumulation of statistically organized data, but Kampo medicine has a long history of 3,000 years, and Kampo medicine has been systematized based on such many years of clinical experience. I) It is true that the same “proof” is accompanied by a common physiological change, and ii) Kampo treatment based on the evaluation of “proof” is almost common in the same “proof”. And iii) Paying attention to the fact that taking the same prescription of the same herbal medicine is accompanied by a common physiological change, a study was conducted to make effective use of human genome sequence information.

  As a result, the human genome sequence information is effectively used to identify genes that change in expression with the appearance / disappearance of “certification” and / or genes that change in expression due to the use of Kampo medicines, and the expression status of these genes, That is, by measuring the presence or absence of gene expression or a change in the amount, the above-mentioned problems were solved, and the development of genes used in the diagnosis of Kampo medicine and its utilization technology was successful, thereby completing the present invention.

  According to the present invention, a comprehensive analysis of gene expression that fully utilizes human genome information can be used to evaluate "test" in Kampo medicine, select Kampo medicines, or determine efficacy before taking Kampo medicines. By applying it to the analysis, it is possible to give objectivity to diagnosis in Kampo medicine, which has conventionally relied solely on the rule of thumb. As a result, the reliability and reproducibility of diagnosis in Kampo medicine can be significantly improved.

  In other words, the present invention is not limited to the experienced and skilled Kampo doctors, that is, for doctors engaged in general medical practice regardless of Western medicine or Kampo medicine, with the instructions of the diagnosis name and prescription unique to Kampo medicine. Diagnosis such as evaluation of a certain “proof” and selection of Chinese medicine can be made quickly and easily.

  Furthermore, the present invention can be applied to a period in which a clear Western medical name, which is a characteristic of Kampo medicine, is not reached, that is, a semi-healthy state that can be said to be a pre-stage of the disease. The treatment can be improved to prevent the disease.

  In addition, even in a case where even a skilled Chinese doctor is difficult to diagnose, by using the present invention in a complementary manner, a more accurate and reliable diagnosis is possible.

  Hereinafter, embodiments of the present invention will be described in detail.

I. Gene identification:
(A) A gene useful for diagnosis in Kampo medicine according to the present invention, specifically, a gene that can be used as a target gene in Kampo medicine diagnosis described later is a gene whose expression varies depending on a specific measurement standard of Kampo medicine. is there.

  The above-mentioned measurement standards include (a) appearance / disappearance of specific “certification” in Kampo medicine, (b) taking specific Chinese medicine, (c) ease of effectiveness of specific Chinese medicine, or (d) these (a) To (c) at least two arbitrary combinations are employed.

(A) (i) “Testimony” is an original measure in Kampo medicine, and has been used by experienced and experienced Kampo doctors to show symptoms and findings (mental state or general condition) , Physical condition, tendency, etc.) are the diagnostic names unique to Kampo, that is, the diagnostic results obtained from the physical characteristics of the Kampo's original medical measure.

  “Testimony” indicates various physical characteristics obtained by examining a patient. There are several combinations of these proofs, and there is a “combination of proofs” that shows the physical characteristics of people who are likely to benefit from each herbal medicine. This “combination of proofs” is called “proof of herbal medicine”.

(Ii) The state of mind and body changes every moment, and the patient's “testimony” also changes over time. Thus, the “proof” is an index indicating how the patient has deviated from the healthy state before the illness becomes ill. If the misalignment is known, the direction of returning to the original can also be understood. In this sense, the “certification” is a Kampo-specific diagnosis name as described above, that is, a diagnosis result, and also a treatment instruction.

  Therefore, the patient's “certification” is called the name of the prescription, that is, the name of the prescription Chinese herbal medicine, and is called “the proof of Kososan”, “the proof of Hangekoubokuto”, etc. In some cases, it is expressed as a “proof” of Chinese medicine, and this is the “proof” of the above-mentioned medicine.

  Kampo medicine is also referred to as “medicine” medicine. In Kampo medicine, it is most important to evaluate and diagnose the above “certification”. We use all five senses of Kampo doctors and collect information on the patient's physical and mental condition through the so-called “four examinations” (Shishin) and make a comprehensive judgment.

  “Four medical checkups” is a Kampo original medical checkup method based on the subjective experience of Kampo doctors. “Waishin”, “Interview (Bunshin)”, “Interview (Monsin)” It consists of four methods of “diagnosis (setting)”.

  Here, “observation” is an examination method that visually determines the facial expression of the patient, the gloss of the face and skin, movement, tongue, etc., and “hearing” refers to the patient's voice based on hearing and smell. “Interrogation” is an examination method that asks the patient about his / her medical history and subjective symptoms according to questions to the patient, and “cutting”. Is a diagnosis method in which a patient's body is actually touched with a hand and judged from the patient's pulse, chest / abdominal hardness, heat / cool, elasticity, and the like.

(B) Kampo medicines, also referred to as Kampo medicines, are complex mixtures containing a number of medicinal ingredients composed of a combination of herbal medicines such as plants, minerals, and animal proteins. , Work on the whole body.

  Specifically, these herbal medicines have been selected and systematized based on many years of clinical experience as the best prescriptions for each of the above-mentioned “proofs”, and are directly expressed as “proofs” of the above-mentioned drugs. There are numerous Chinese herbal medicines, including Kousousan, Hangekoubokuto, Yokukansan and Yokukansankachinpihange.

  Thus, the above target genes are identified as follows using the appearance / disappearance of these “proofs”, the use of Kampo medicines, the effectiveness of Kampo medicines, or any combination of these at least two as its metric. The

(1) First, a person corresponding to the above-mentioned specific reference means, i.e., i) a person who presents a specific “test” diagnosed (evaluated / judged) by an experienced Chinese medicine doctor, ii) a specific Chinese medicine Biological samples are taken from those who have taken iii) or who fall under iii) i) and ii).

  The biological sample is not particularly limited as long as RNA (ribonucleic acid) can be collected. For example, a tissue sample, blood (blood cell), cerebrospinal fluid (spinal fluid cell) and the like are used.

(2) Extract and purify total RNA from the collected biological sample.
There are no particular restrictions on the method for extracting and purifying total RNA, but it is transcribed from DNA (deoxyribonucleic acid) in nucleated cells such as RNase in the sample, lymphocytes and leukocytes in the sample. In order to immediately stop RNA synthesis, it is preferable to perform treatment by a method using a protein denaturant such as AGPC method (acidic guanidine / phenol / chloroform method). Recently, a dedicated blood collection tube filled with an organic acid-based drug is commercially available, and this can also be used.

(3) The gene expression state of total RNA is analyzed to identify the target gene.
In gene expression analysis, total RNA is used as it is, and mRNA (messenger RNA) is isolated and used from a purified total RNA preparation. If a sufficient amount of mRNA (messenger RNA) is not obtained, it is amplified appropriately.

  The gene expression analysis method using the mRNA as a sample is preferably a method capable of comprehensive analysis, and the DNA chip method, the FDD method (fluorescent differential display method), and the like can be used effectively.

  That is, in gene expression analysis by the DNA chip method, mRNA samples are labeled with different fluorescent dyes before and after taking Chinese medicine, or when “proof” is given, and then DNA chips (unspecified many Specific competitive binding reaction to the probe gene on the substrate), or a synthetic oligonucleic acid having a sequence of the gene or a fragment thereof or the gene on the support as a probe gene, A gene is identified by reading the expression level of the gene.

  On the other hand, in gene expression analysis by the FDD method, oligo dT primers to which fluorescent dyes have been added using all mRNAs in RNA samples before and after taking Chinese medicine as well as when "proof" is given as a template. After synthesizing cDNA (complementary DNA) by the reverse transcription reaction used, this primer is used as an anchor primer, and a PCR reaction is performed with a primer having an arbitrary short-chain mixed sequence with reduced specificity to obtain a plurality of gene amplification products. Subsequently, these gene amplification products are subjected to electrophoresis, and after screening for genes whose expression level is changed, isolation, cloning, base sequence or a part of the base sequence is determined, and then the gene is verified Is identified.

  The present invention does not limit the gene expression analysis method, and any method possible at that time may be adopted.

(B) Specific example:
Next, as specific examples of this embodiment, among Chinese medicines that affect mental illnesses and human emotions, various Chinese medicines that are particularly effective for mental stress (for example, Kousosan, Hanka Kobakuto) With the above metric as the above metric, the identification of target genes whose expression varies according to these metric, the “proof of Kosyosan” and its Kampo medicines in later examples An explanation will be given by taking an example of Kosyosan.

(A) First, an expression gene for “proof of Kosyosan” and its Chinese medicine, Kosyosan, is identified.
As an effective agent to eliminate the “proof of Kosyosan” for 100 subjects who have been diagnosed by experienced Chinese medicine doctors as having a common “proof of Kosyosan” revealed Prescribe a powder and take it according to the usage and volume of the medicine for a specified period. In general, the prescribed period to be taken varies depending on the type of “certification” indicated and the type of Chinese medicine to be taken, but it is preferable to take the period when there is a difference in the expression of “certification”. In the case of Kosyosan, ask them to take it for 2-4 weeks.

  Peripheral blood is collected from the subject immediately before taking Kosyosan, that is, when the “proof of Kosyosan” appears and when the “proof of Kosyosan” disappears. In this case, when it is difficult to collect blood when the “proof of Koshosan” disappears, blood collection at the time when it is considered that the “proof of Koshosan” has disappeared may be used. Furthermore, blood collection at an arbitrary time point until the “proof of Koshosan” disappears may be added.

  Next, total RNA (total ribonucleic acid) is extracted and purified from the collected blood. As described above, the extraction / purification method in this case is the synthesis of RNA transcribed from DNA (deoxyribonucleic acid) in nucleated cells such as lymphocytes and leukocytes in the collected blood sample as well as RNase after blood collection. In order to stop the treatment immediately, it is treated by a method using a protein denaturant such as AGPC method (acid guanidine / phenol / chloroform method).

  Blood collected at the time when the "proof of Kosyosan" is displayed and when it disappears, or total RNA obtained before and after taking Kosyosan can be processed and analyzed in subsequent stages If the recovered amount can be obtained, it can be used for analysis as it is, but it is preferable to use mRNA (messenger RNA) isolated from the sample. If a sufficient amount of mRNA (messenger RNA) cannot be obtained, it can be amplified. The method for amplifying mRNA is not particularly limited, but NASBA method (Nucleic Acid Sequence-Based Amplification), TMA method (Transcription-Mediated Amplification), TRC method (Transcription-Reverse Transcription Concerted Reaction), 3SR method (Self-Sustained Sequence Replication) can be used.

  Next, genes that differ in the expression state, that is, the presence or absence of the expression or the amount thereof at the time of expression and disappearance of the “proof of Kosousan”, or before and after the ingestion of Kosousan are identified. As the gene expression analysis method using the above-mentioned mRNA as a sample, a method capable of comprehensive analysis is preferable. As described above, for example, a DNA chip method or an FDD method can be used, but here, the FDD method is used.

  In other words, in this gene expression analysis by the FDD method, all the mRNAs in the RNA sample obtained at the time when the “proof of Kosyosan” is displayed and when it disappears, or before and after taking Kousosan are used as templates. After synthesizing cDNA by reverse transcription using an oligo dT primer to which a fluorescent dye was added, this primer was used as an anchor primer, and a PCR reaction was performed with a primer having an arbitrary short-chain mixed sequence with reduced specificity. A gene amplification product is obtained. Subsequently, these gene amplification products are subjected to electrophoresis, and after screening for genes whose expression levels have changed before and after taking Kousosan, isolation, cloning, base sequence determination, and comparison with the database are performed. Identify genes. The identification of the gene can also be performed by specifying the position of the recognition sequence on the gene by the above primer sequence or several kinds of restriction enzyme digestion reactions. The feature of this method is that an unknown gene can be found and the expression analysis of the gene over time is possible.

  As described above, it is possible to identify a gene whose expression varies with the appearance / disappearance of “proof of Kosyosan” or before and after taking Kosyosan. In other words, it can be effectively used as a target gene in diagnosis such as evaluation of “proof” and selection of Chinese medicine.

  As will be described later in the examples described later, by adopting the test method as described above, a gene that changes in expression in relation to “proof of Kosyosan” and the expression of “proof of Kosyosan” are displayed. In addition to this, genes that change in expression due to the medicinal effects of Kousousan, and in addition to this, genes that change in expression after taking Kousousan, regardless of "proof of Kousousan" it can.

(B) Similarly, various Chinese herbal medicines that are highly effective against the remaining mental stress, that is, Hanshin-koboku-to in Example 3 later, or Each “certificate” such as Katsueka Kyuroku Oyakuyu, and Hanshin-koboku-yu, Yokukansan, Yokukansan-Kenshin-Hakka, Kamizo Harukasan, and Keishika Kyuroku Oyster, which are prescribed for each of these “certifications”. For hot water, using these as the measurement standard, the target gene whose expression varies depending on these measurement standards in the same procedure as the above-mentioned “proof of Kosyosan” and the method of identifying the expressed gene for the Chinese medicine, Kousosan Are identified respectively.

II. Gene expression analysis platform:
(A) The genes identified as described above are classified according to a predetermined standard (for example, function) and used as a basis for gene expression analysis, thereby making it easier and more reliable for later Kampo medical diagnosis. Can be done.

  That is, although not specifically shown, the gene expression analysis platform according to the present invention has a plurality of target genes identified by the above-described method arranged on the support by a conventionally known DNA array technology or DNA chip technology. And fixed. As the support, a flat support base made of glass, silicon, or the like is used, and the shape and dimensions thereof are set corresponding to the number of target genes arranged and arranged thereon. Further, the number of target genes is preferably 5 or more in order to increase the accuracy of the test.

(B) Specific example:
The gene expression analysis platform as a specific example of this embodiment is the above-mentioned various Chinese herbal medicines that are highly effective in stress (Kousosan, Hanka-kobaku-to, Yokukansan, Yokukansan-kakin-summer, Kamikan Harukasan and Katsura The expression genes of “Certificate” and / or the formula prescribed for each of these “Certification” are aligned on the support by a conventionally known DNA array technology or DNA chip technology. And fixed.

  Thus, in order to perform gene expression analysis using the thus constructed base for gene expression analysis, a measurement target sample obtained from a subject and a sample prepared from a comparative control person prepared in advance are used. After labeling mRNA samples extracted and purified as described above with different fluorescent dyes, specific competitive binding reactions are made to the probe genes on the above-mentioned gene expression analysis platform, and the expression of individual genes is performed with a fluorescence scanner. Read the amount.

  In this way, by incorporating gene expression analysis using the gene expression analysis platform into Kampo medicine, information on the expression patterns of a large number of genes can be obtained in a single measurement, and each individual's changing "proof" Diagnosis of the presence or absence of the appearance of the can be more appropriate and quick.

III. Kampo medical diagnosis:
(A) By using the various genes identified in I above as target genes as they are or as the basis for gene expression analysis in II above, it is possible to objectively and easily and reliably carry out subsequent Kampo medical diagnosis. it can.

(A) For example, in order to evaluate a patient's “proof” in the field of Kampo medicine, the expression state of a target gene in a measurement sample obtained from the patient is measured, and the measurement result is used as a comparative sample as an evaluation standard. The presence or absence of expression of the “test” of the subject is evaluated by comparing with the expression state of the target gene.

  In this case, for the method of measuring the presence or amount of target gene expression, such as RNA extraction in a measurement sample obtained from a patient, purification of mRNA, amplification as necessary, etc., identification of the target gene in I above This is the same as the method described for.

  The target gene in the comparative sample is appropriately selected from the target genes obtained in I above. It is desirable that the gene to be selected is selected from a gene group classified in advance according to a predetermined standard (for example, function).

  The gene expression analysis performed in this way is to objectively determine whether or not the “proof” is expressed by comparing the gene expression level of the patient with the level of gene expression of a comparative control person who does not express the “proof”. Can be evaluated. In addition, by observing the degree of gene expression between measurement samples collected daily in the same patient, the expression / disappearance of the “proof” can be objectively grasped.

  In addition, as a method of constituting a comparative control person for whom the above “proof” is not expressed, for example, for a plurality of healthy persons who have not received drug therapy, the age distribution and gender composition ratio of the average medical examination patient and There is a method of configuring in the same way as the gender composition ratio, or by dividing the age range and gender of multiple healthy subjects who have not received drug therapy. For the sake of accuracy, the latter configuration method Is desirable.

  In order to increase the accuracy of evaluation, preferably 5 or more genes are selected. The greater the number of genes to be analyzed, the easier it is to evaluate “proof” that is affected by multiple factors, and the objectivity and accuracy of the evaluation are further improved.

  Furthermore, when comparing the measurement result of the expression state of the target gene in the measurement sample with the expression state of the target gene in the comparison sample, when comparing a plurality of genes, it is also possible to compare as a pattern. is there.

  Also, in this case, by using the gene expression analysis platform of II described above, information on the expression patterns of a large number of genes can be obtained in one measurement, and the expression of “proof” that changes for each individual patient can be expressed. Diagnosis of the presence or absence, etc. can be performed more appropriately and quickly. In other words, in a gene expression analysis platform in which a large number of genes (gene groups) identified in relation to a specific “evidence” are aligned and immobilized as an expression pattern, each patient's individual disease type and disease Therefore, it is possible to select a more appropriate treatment method.

(B) In addition to the original method of evaluating the “proof” described in (a) above, the fact that Chinese medicine has almost no side effects, and using the gene identified in I above Thus, the use of Kampo medicines as a kind of test drug, that is, the way for the adaptive diagnosis of Kampo medicines will be opened.

  That is, for example, in a general health checkup such as a medical checkup, the expression state of a target gene in a measurement sample obtained from a patient (subject) by a blood test or the like is measured, and the measurement result is used as a comparative sample as an evaluation standard. By comparing with the expression state of the target gene in each, the presence or absence of a gene expression pattern peculiar to traditional Chinese medicine is observed for each examinee, and the health condition of the examinee is evaluated and determined.

  In other words, if the presence of a gene expression pattern specific to a specific Chinese medicine is observed for a certain patient, this Chinese medicine with this observed expression pattern is expected to have an effect on this patient. Therefore, the examinee evaluates and judges that the corresponding Chinese herbal medicine has the physical and emotional characteristics and symptoms that are the target.

  In this case, RNA extraction in the measurement sample obtained from the above-mentioned examinee, purification of mRNA, amplification as necessary, and the method for measuring the presence or amount of target gene expression, A method for comparing a plurality of genes when comparing with the expression state of a target gene in the comparative sample, or a method for identifying and selecting a target gene in a comparative sample as an evaluation standard is described in (a) above. It is the same as the method.

(B) Specific example:
Next, as a specific example of the present embodiment, the “identification” of various Kampo medicines related to stress listed above as a specific example, and / or the genes identified regarding the anti-stress Kampo medicines prescribed for these “proofs” are targeted. The diagnosis of Kampo medicine used as a gene as it is or as a base for the above gene expression analysis will be described.

  Here, a case will be described in which the anti-stress Chinese medicine in (b) of (A) is used as a kind of test drug in a health checkup such as a medical checkup.

(1) Classification of mental states in modern medicine:
In modern medicine, mainly Western medicine, the method of measuring a person's heart, that is, the mental state, is centered on the score obtained by the questionnaire method. In other words, the patient reports the subjective symptoms to the doctor, which is the basis for the diagnosis by the doctor. For example, the present situation is that the diagnosis of “depression” relies almost exclusively on the chief complaint of the patient. In addition, it is difficult to make an accurate diagnosis even for serious mental illnesses such as schizophrenia that tend to cause harm and other self-harm. For this reason, people who seem to be normal at times seem to suddenly commit suicide and surprise others.

  Even in general companies, interest in stress has increased greatly in recent years, and stress management is especially emphasized in corporate health management. However, mental illness rarely shows abnormal values in laboratory data such as blood tests, and the actual situation depends on the person's complaint. In the present age when the number of suicides per year is increasing significantly, there is a need for a simple evaluation method of the spirit.

(2) Classification of mental states in Kampo medicine:
There are a group of Chinese herbal medicines called “Kiyaku”. In Kampo medicine, the mental state of a person is categorized into Kiutsu, Kikai (Kigaku), and Stuck (Kitai), etc. In the five organs classification, the liver (Kang) is angry, the heart (Shin) is joyful, and the spleen (Hi) is sad, lungs (high) are sad, and kidneys (jin) are surprised. Kampo has a system in which such organs and emotions correspond, and it is used for Kampo diagnosis and treatment.

  Among these, the use of the gene according to the present invention enables a new classification of mental states based on modern evidence and referring only to a portion with a scientific basis.

  In other words, the relationship between mental state and herbal medicine has been said since ancient times. However, these relationships were used as a procedure for subjective medical examinations by Kampo doctors, as described above, and were considered to have no scientific basis. This mental state classification can classify a person's heart (emotion, stress) only by a simple blood test by comparing with the expression of the gene according to the present invention.

(3) Gene of the present invention and herbal medicine As a specific example of this embodiment, the “proof” of various Chinese medicines related to mental stress listed above and / or the anti-stress Chinese medicine prescribed for these “proof” A new classification of mental states can be made by using the genes identified for drugs as target genes and looking at the indications of these Chinese medicines.

  That is, this mental state classification is not subjective from the patient, such as a questionnaire method, but by using the gene according to the specific example of this embodiment, the expression of the gene is examined through blood tests, and the objective state is classified. Can be evaluated for the first time.

  For example, Yokukansan is characterized by a mental state that is always frustrating. Suppose that the expression of a gene in the blood of a person shows that Yokukansan is effective before taking herbal medicine. From this, it is evaluated that the characteristic of the person's spirit at that time is a frustrating type that is characteristic of Yokukansan.

  In other words, the type of mental stress and emotion that is unique to the patient is classified by having the above-mentioned Chinese herb medicine play a role like a reagent. By doing this, it is not possible to think of whether or not it is stressed, but also the type, which was not thought of by common sense in modern medicine. Furthermore, since a Chinese medicine that can be said to be a specific medicine against the stress can be prepared individually, the flow from diagnosis to treatment in Kampo medicine can be achieved.

  In Kampo medicine, mental stress is classified finely. For example, even if the same mental pressure (stress) is applied, there are two types: depression type and repulsion type. In addition, there are various types such as a type in which symptoms of stomach pain occur due to mental stress, a type in which a headache occurs, or a type in which diarrhea occurs.

    Those who represent anti-stress, such as Koso-san, Hanka-san, Yokukansan, Yokukansan-kashin-han, Kami-an-haruka-san, and Katsue-ka-ryukyou-to, as in the specific example of this embodiment described above By exploring and identifying the predicted genes of drugs, it is possible to classify mental stress that was not possible until now.

  Based on the classification of mental stress, it becomes possible to use an anti-stress Chinese medicine as a kind of test drug with the gene identified for the above-mentioned anti-stress Chinese medicine as a target gene.

  Specifically, in a general health checkup such as a medical checkup, the expression state of a target gene in a measurement sample obtained from a patient by blood test or the like is measured, and this measurement result is used as the evaluation criterion (I) Observe by comparing with the expression state of the target gene identified for the anti-stress Chinese medicine in B).

  Then, if the expression of a gene characteristic of the above-mentioned anti-stress Chinese medicine is observed for a certain medical examinee, the traditional Chinese medicine for which the anti-stress Chinese medicine of this observed gene expression is expected to have an effect on this medical examinee Therefore, the examinee evaluates and judges that the corresponding anti-stress Chinese medicine has the target physical and emotional characteristics and symptoms.

  In this case, RNA extraction in the measurement sample obtained from the above-mentioned examinee, purification of mRNA, amplification as necessary, and the method for measuring the presence or amount of target gene expression, A method for comparing a plurality of genes when comparing with the expression state of a target gene in the comparative sample, or a method for identifying and selecting a target gene in a comparative sample as an evaluation standard is described in (a) above. It is the same as the method.

  Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to these examples.

Example 1
In this example, genes whose expression varies with the appearance / disappearance of “proof of Koshosan” are identified by expression analysis using the FDD method.

  Thirty subjects, who were judged to have "proof of Kosyosan" as a result of an examination by an experienced Chinese medicine doctor, were administered Kasousan for 2 weeks according to their dosage and administration.

  Before and after taking Kousousan, blood from the subject is collected in a dedicated blood collection tube (PAXgene RNA vacuum blood collection tube) (trade name: manufactured by QIAGEN), and “PAXgene Blood RNA Kit” (product name: manufactured by QIAGEN) is used. The total RNA preparation was prepared according to the device manual, except that the RNA preparation was applied to “PAXgene RNA Spin Column” (trade name: QIAGEN) in order to avoid DNA contamination. The DNase treatment with “RNase-Free DNase Set” (trade name: manufactured by QIAGEN) was performed on the spin column membrane.

  After four weeks of taking Kousousan, four subjects by Kampo doctors made 18 subjects whose “proof of Kousousan” disappeared markedly as a response group (Responder), and the disappearance of “proof of Kousousan” was confirmed. Twelve subjects who were not able to do so were treated as non-responders, and the total RNA preparations before and after taking Kousousan were mixed in groups at the same mixing ratio.

  Identification of genes whose expression fluctuates before and after taking Kousousan was performed by the FDD method (fluorescent differential display method).

  First, using a mixed total RNA preparation as a material, an oligo d (T) primer (with a poly T sequence (Poly T sequence) added with A, C, or G) and “SuperScript TM First-strand Synthesis System” cDNA synthesis was performed using “for RT-PCR” (trade name: manufactured by GIBCO BRL). That is, the PCR reaction by the FDD method is a technique for amplifying a gene sequence sandwiched between two primers using an oligo d (T) primer and a known sequence primer having a length of 10 bases. 'The oligo d (T) primer with A added to the end performs PCR with SEQ ID NO: 50 to 129 in the sequence listing, and the one with C added also between SEQ ID NO: 130 to 209 in the sequence listing. PCR was performed in the same manner, and those with G added were subjected to PCR between SEQ ID NOS: 210 to 289 in the Sequence Listing. Therefore, a total of 240 combinations of PCR reactions were performed.

  Next, using these synthesized first strand cDNAs as templates, an oligo d (T) primer (manufactured by Nippon Flour Mills) and a known sequence primer (manufactured by OPERON TECHNOLOGY), which are fluorescently labeled with Texas-Red, are used. PCR was performed. PCR primer sequences that satisfy the following conditions were used.

  That is, i) no inter-primer primer dimer is formed, ii) no intra-primer primer dimer is formed, iii) no intra-primer self-complementary sequence is included, and iv) DNA between the primer and template DNA is 40-70. There are four conditions of being in the range of ° C.

  In addition, the specificity was reduced by setting the primer chain length to about 10 bases so that the number of genes amplified by one primer pair was about 100 to 200. As described above, the respective primer sequences in this example are represented by SEQ ID NOs: 50 to 289 in the sequence listing.

  For PCR, 1 μL of the first strand DNA was added to 19 μL of the mixture having the composition shown in Table 1, and the first cycle was 94 ° C. for 3 minutes, 40 ° C. for 5 minutes, and 72 ° C. for 5 minutes, and the second cycle. Thereafter, 24 cycles of 94 ° C. for 20 seconds, 40 ° C. for 2 minutes, and 72 ° C. for 1 minute were performed, followed by reaction at 72 ° C. for 5 minutes.

  After PCR, add 2.0 μL of the PCR product preparation to an equal volume of loading buffer (98% (V / V) formamide, 1.0 mM EDTA, 0.01% (W / V) Methyl violet was added and treated at 80 ° C. for 2 minutes to obtain a sample for electrophoresis. Electrophoresis was performed using a Hitachi DNA sequencer (trade name: manufactured by Hitachi, Ltd.) and a denatured acrylamide gel (6% (W / V) “Long Rangar” (trade name: manufactured by Takara Shuzo Co., Ltd.), 6.1 M urea (Urea). 1.2 × TBE), and the sample and molecular weight marker (100 base ladder marker from 100 to 1000 bases) were electrophoresed and separated. Thereafter, a DNA band was detected with a fluorescence image scanner (Hitachi Software “FMBIO” (trade name: manufactured by Hitachi, Ltd.)). In order to facilitate subsequent analysis, electrophoresis is preferably performed by applying a PCR product obtained from a mixed total RNA preparation obtained before and after taking Kosousan to adjacent electrophoresis lanes.

  After electrophoresis, select the migration band, that is, the gene fragment, where the expression changes before and after taking Kousousan and the expression changes between the reaction group and non-reaction group as much as possible, and the chain length is defined as the molecular weight marker. Obtained by comparison. The selected gene fragment was cut out from the gel after electrophoresis and placed in a 1.5 mL tube. Purified water (30.0 μL) was added to the tube and left in a freezer with a room temperature of −80 ° C. for 10 minutes. The tube after leaving was taken out and stirred for 10 minutes at room temperature (room temperature) using a vortex mixer to dissolve the sample. This operation was repeated twice to extract DNA.

  Next, 1.0 μL of each extraction solution was used as a template, and the target gene fragment was re-amplified using the same PCR reaction conditions as described above.

  Subsequently, the gene fragment after reamplification was cleaved with a restriction enzyme. The restriction enzyme used here is preferably selected to have a relatively few restriction enzyme recognition sequences on the gene. In this example, each was cleaved alone using a restriction enzyme with 4-base recognition such as AluI and HaeIII. The gene fragment obtained by cleavage was electrophoresed using a Hitachi DNA sequencer, the fragment on the fluorescently labeled side was visualized, and the fragment length after cleavage was calculated from the relative migration distance from the molecular weight marker. . By subtracting this fragment length from the chain length amplified by the oligo d (T) primer and the known sequence primer, the target gene has the known sequence primer sequence and the restriction enzyme recognition sequence at a distance of the above chain length difference. Will exist.

  Based on the positional information of the base sequence obtained in this way, genes having homologous surnames were searched from existing database sequences. As databases, NCBI Unigene and Genbank were used. These searches were conducted not only for genes that are homologous to known sequences, but also for genes that are complementary to known sequences. This is because it is necessary to consider that the direction of the known sequence is unknown and the direction in which the gene is registered in the database is unknown.

  Also, the sequence information obtained by direct sequencing of the most amplified gene fragment to complement the above search was also checked against the database, that is, a normal blast (BLAST) search was performed to identify the target gene. .

  As a result, when a homologous gene cannot be obtained, it is highly likely that a gene present in the fragment or a gene containing the fragment is novel.

  Table 2 shows the Unigene number (Unigene #) and Genbank number (Genbank #) and gene names identified through the above process.

  In the gene list of Table 2, the fragment names C076-430 and G046-310 in the fragment name column can be identified by sequence prediction from the restriction enzyme recognition sequence position information, but are genes that cannot be directly sequenced. is there.

  In the database number column, the number starting with Hs. Is a Unigene number (Unigene #), and the other numbers are Genebank numbers (Genbank #).

  Furthermore, in the variation status column, the genes indicated as A-down and A-up are genes involved in the medicinal effects of Kosyosan, and are expressed in response to the use of Kosyosan in the reaction group of Kosyosan. Decreased expression is expressed as down, expression increased as up, and genes expressed as B-down and B-up are those who have evidence of Kousousan before taking Kosasan (Responder) The gene that is highly expressed in up is expressed as up, the gene that is expressed in low is expressed as down, and the genes expressed as C-down and C-up are used regardless of the evidence of Those whose expression is decreased in (2) are expressed as down, and those whose expression is increased are expressed as up.

  From the above experimental results, there is a clear difference in gene expression between the reaction group that showed an effect on Kousousan and the non-reaction group that did not show it. A gene expression pattern was found. As a result, it was suggested that a reaction group with good response to Kosousan could be known in advance.

  In addition, it was suggested that the characteristic genes include genes showing the mechanism of action of Kosousan that was unknown until now. Furthermore, the number of genes exhibiting gene expression characteristics was 53 within a range of 50 to 60, which is considered easy to analyze.

Example 2
In this example, a gene whose expression changes with the appearance / disappearance of “proof of Koshosan” was identified by expression analysis using the DNA chip method.

  Thirty subjects, who were judged to have demonstrated “proof of Kosyosan” by examination by experienced Chinese medicine doctors, were administered Kosyosan for 2 weeks according to their usage and dosage.

  Before and after taking Kousousan, blood is collected from the subject in a dedicated blood collection tube “PAXgene RNA vacuum blood collection tube” (trade name: manufactured by QIAGEN), and using “PAXgene Blood RNA Kit” (trade name: manufactured by QIAGEN) A total RNA preparation was prepared according to the instrument manual. However, in order to avoid DNA contamination, the RNA preparation is applied to the “PAXgene RNA Spin Column” (trade name: QIAGEN) and then “RNase-Free DNase Set” on the spin column membrane. DNase treatment was performed using (trade name: QIAGEN).

  After four weeks of medical examination by a Chinese medicine doctor after two weeks of taking Kousousan, 18 subjects who had noticed the disappearance of “Kousousan” became the reaction group (Responder), and the disappearance of “Kohyosan” was confirmed. Twelve subjects who did not respond as non-responder, total RNA preparation before taking the reaction group and before taking the non-reaction group, and total RNA preparation before and after taking the reaction group Each was mixed in groups at the same mixing ratio.

  Identification of genes whose expression fluctuated before taking the reaction group, before taking the non-reaction group, or before and after taking the reaction group was performed by the DNA chip method.

  First, synthesis of the first strand of cDNA ("SuperScript IIRnaseH-") using an oligo d (T) primer (Bioscience) to which a promoter sequence of T7 RNA polymerase was added using 5 micrograms of the mixed total RNA preparation as a material. (Trade name: manufactured by Invitrogen).

  Next, heavy chain cDNA (having a T7 RNA polymerase promoter sequence at the 3 ′ end) was prepared from these synthesized first strand cDNAs, and T7 RNA polymerase (“MEGAscript T7” (trade name) was present in the presence of dUTP allylamine derivative. : Ambion))) to amplify cRNA.

  Thereafter, this cRNA was reacted with a cyanine dye “N-succinimide ester” (trade name: manufactured by Perkin Elmer) and fluorescently labeled. The unreacted cyanine dye not subjected to labeling was “QIAquick column”. (Trade name: manufactured by QIAGEN).

  Hybridization reaction using a DNA chip was performed in two combinations. The first combination is: RNA before taking Kousousan in the Kousousan reaction group (labeled with cyanine dye Cy3) / RNA before taking Kosayosan in the non-Kohyosan group (labeled with cyanine dye Cy5) The second combination is the RNA before taking the Kousaosan in the Kosasan group (labeled with the cyanine dye Cy3) / after taking the Kosayosan in the group (labeled with the cyanine dye Cy5). Performed in combination with RNA. Cyarray labeled RNA and Cy5 labeled RNA are mixed in equal amounts, and an oligo DNA (60 base length) corresponding to 18,716 gene is immobilized ("Whole Human Genome Oligo microarray" (trade name: manufactured by Agilent)) In contrast, a hybridization reaction was performed at 65 ° C. for 17 hours.

  After the hybridization reaction, unreacted substances were washed away with a washing solution, and scanned with a confocal laser scanner (“Agilent G2565A” (trade name: manufactured by Agilent)). The fluorescence intensity after scanning was subjected to background correction and analysis after global normalization.

  After the hybridization reaction, the fluorescence intensity of the non-reactive group before Kousosan (labeled with cyanine dye Cy5) is the same as that before the Kousosan reaction group (labeled with cyanine dye Cy3). Table 3 shows the Unigene numbers of genes that are 0.5 times or less, and Table 4 shows the Unigene numbers of genes that are 2 times or more.

  In addition, the fluorescence intensity after taking Kousousan in the Kousousan group (labeled with cyanine dye Cy5) is 0.5 times the fluorescence intensity before taking Kosayosan in the group (labeled with cyanine dye Cy3). The Unigene numbers of the following genes are shown in Table 5, and the Unigene numbers of genes twice or more are shown in Table 6.

  In addition, in the DNA chip analysis data of Kousosan, genes whose expression is decreased (expression is 0.5 times or less) in subjects who have “proof of Kousosan” (expression is 2 times or more) More than 70 were found. From this, it is expected that the efficacy of Kosousan will become apparent at the genetic level.

Example 3
In this example, genes that change in expression with the appearance / disappearance of “proof of Hanka Kobokuto” were identified by the expression analysis by the FDD method in the same manner as in Example 1.

  Thirty subjects, who were judged to have been presented with "proof of Hanka Kokakuto" by medical doctors with experience, received Hanka Kokokuto for 2 weeks according to the dosage and administration.

  Before and after taking Hanshin Koboku-to, blood from the subject was collected in a dedicated blood collection tube (PAXgene RNA vacuum blood collection tube) (trade name: manufactured by QIAGEN), and “PAXgene Blood RNA Kit” (trade name: manufactured by QIAGEN) The total RNA preparation was prepared according to the device manual, except that the RNA preparation was applied to “PAXgene RNA Spin Column” (trade name: QIAGEN) in order to avoid DNA contamination. The DNase treatment with “RNase-Free DNase Set” (trade name: manufactured by QIAGEN) was performed on the spin column membrane.

  After 4 weeks of taking Hanshin Koboku-yu, four subjects by Kampo doctors made 16 subjects who had “disappearance of“ Hancatsu-Koboku-yu ”” as the response group (Responder), and the disappearance of “Hankatsu-Koboku-no-Yu” was confirmed. The 14 test subjects who were not able to perform were made into the non-responder group, and each total RNA sample before and after taking Hanshin Kobokuto was mixed for every group by the same mixing ratio.

  Identification of genes whose expression fluctuates before and after taking Hanka-kobokuto was performed by the FDD method (fluorescent differential display method).

  First, using a mixed total RNA preparation as a material, oligo d (T) primer (with 3 'end of poly T sequence (PolyT sequence) added with A, C or G) and "SuperScript TM First-strand Synthesis System" cDNA synthesis was performed using “for RT-PCR” (trade name, manufactured by Invitrogen). That is, the PCR reaction by the FDD method is a technique for amplifying a gene sequence sandwiched between two primers using an oligo d (T) primer and a known sequence primer having a length of 10 bases. 'The oligo d (T) primer with A added at the end was subjected to PCR with SEQ ID NOS: 339 to 418 in the sequence listing, and the one with C added was between SEQ ID NOS: 419 to 498 in the sequence listing. PCR was performed in the same manner, and those with G added were subjected to PCR with SEQ ID NOs: 499 to 578 in the sequence listing. Therefore, a total of 240 combinations of PCR reactions were performed.

  Next, using these synthesized first strand cDNAs as templates, an oligo d (T) primer (manufactured by Nippon Flour Mills) and a known sequence primer (manufactured by OPERON TECHNOLOGY), which are fluorescently labeled with Texas-Red, are used. PCR was performed. PCR primer sequences that satisfy the following conditions were used.

  That is, i) no inter-primer primer dimer is formed, ii) no intra-primer primer dimer is formed, iii) no intra-primer self-complementary sequence is included, and iv) DNA between the primer and template DNA is 40-70. There are four conditions of being in the range of ° C.

  In addition, the specificity was reduced by setting the primer chain length to about 10 bases so that the number of genes amplified by one primer pair was about 100 to 200. As described above, each primer sequence in this example is represented by SEQ ID NOs: 339 to 578 in the sequence listing.

  For PCR, 1 μL of the first strand DNA was added to 19 μL of the mixture having the composition shown in Table 1, and the first cycle was 94 ° C. for 3 minutes, 40 ° C. for 5 minutes, and 72 ° C. for 5 minutes, and the second cycle. Thereafter, 24 cycles of 94 ° C. for 20 seconds, 40 ° C. for 2 minutes, and 72 ° C. for 1 minute were performed, followed by reaction at 72 ° C. for 5 minutes.

  After PCR, add 2.0 μL of the PCR product preparation to an equal volume of loading buffer (98% (V / V) formamide, 1.0 mM EDTA, 0.01% (W / V) Methyl violet was added and treated at 80 ° C. for 2 minutes to obtain a sample for electrophoresis. Electrophoresis was performed using a Hitachi DNA sequencer (trade name: manufactured by Hitachi, Ltd.) and a denatured acrylamide gel (6% (W / V) “Long Rangar” (trade name: manufactured by Takara Shuzo Co., Ltd.), 6.1 M urea (Urea). 1.2 × TBE), and the sample and molecular weight marker (100 base ladder marker from 100 to 1000 bases) were electrophoresed and separated. Thereafter, a DNA band was detected with a fluorescence image scanner (Hitachi Software “FMBIO” (trade name: manufactured by Hitachi, Ltd.)). In order to facilitate subsequent analysis, electrophoresis is preferably performed by applying a PCR product obtained from a mixed total RNA preparation obtained before and after taking Hanka-kobokuto to adjacent electrophoresis lanes.

  After electrophoresis, select the migration band, that is, the gene fragment, where the expression changes before and after taking Hanka-kobokuto, and the expression changes between the reaction group and non-reaction group as much as possible. Obtained by comparison. The selected gene fragment was cut out from the gel after electrophoresis and placed in a 1.5 mL tube. Purified water (30.0 μL) was added to the tube and left in a freezer having a room temperature of −80 ° C. for 10 minutes. The tube after being left out was taken out and stirred for 10 minutes at room temperature (room temperature) using a vortex mixer to dissolve the sample. This operation was repeated twice to extract DNA.

  Next, 1.0 μL of each extraction solution was used as a template, and the target gene fragment was re-amplified using the same PCR reaction conditions as described above.

  Subsequently, the gene fragment after reamplification was cleaved with a restriction enzyme. The restriction enzyme used here is preferably selected to have a relatively few restriction enzyme recognition sequences on the gene. In this example, each was cleaved alone using a restriction enzyme with 4-base recognition such as AluI and HaeIII. The gene fragment obtained by cleavage was electrophoresed using a Hitachi DNA sequencer, the fragment on the fluorescently labeled side was visualized, and the fragment length after cleavage was calculated from the relative migration distance from the molecular weight marker. . By subtracting this fragment length from the chain length amplified by the oligo d (T) primer and the known sequence primer, the target gene has the known sequence primer sequence and the restriction enzyme recognition sequence at a distance of the above chain length difference. Will exist.

  Based on the positional information of the base sequence obtained in this way, genes having homologous surnames were searched from existing database sequences. As databases, NCBI Unigene and Genbank were used. These searches were conducted not only for genes that are homologous to known sequences, but also for genes that are complementary to known sequences. This is because it is necessary to consider that the direction of the known sequence is unknown and the direction in which the gene is registered in the database is unknown.

  Also, the sequence information obtained by direct sequencing of the most amplified gene fragment to complement the above search was also checked against the database, that is, a normal blast (BLAST) search was performed to identify the target gene. .

  As a result, when a homologous gene cannot be obtained, it is highly likely that a gene present in the fragment or a gene containing the fragment is novel.

  From the above experimental results, there is a clear difference in gene expression between the reaction group that showed an effect on Hankasa Kobokuto and the non-reaction group that was not shown. A gene expression pattern was found. This suggested the possibility that a group of reactions with good response to Hanka-kobokuto could be identified in advance.

  It was also suggested that the characteristic genes include genes showing the mechanism of action of Hanka-Kobokuto, which was unknown until now. Furthermore, the number of genes exhibiting gene expression characteristics was 49, which is close to the range of 50 to 60, which is considered easy to analyze.

  Furthermore, from the standpoint of Western medicine, no matter how much the action mechanism or mechanism of why Kampo medicines are effective, there is a fact that the same medicinal effects as the above Kampo medicines cannot be obtained. I don't know what is affected at the decocting stage when prescribing herbal medicine, but it may have a unique medicinal effect that can not be obtained only with the above chemical components, for example something that occurs at the decocting stage It was not possible to elucidate by simple analysis by modern science, such as that the components of the bacterium are effective in bacteria in the intestines. However, starting with the results of the analysis of the expressed genes for Kosyosan and Hanka Kokakuto, we identified the expressed genes for all herbal medicines and their “proofs” by comprehensive analysis methods. It becomes possible to give objectivity to the diagnosis in Kampo medicine that has depended on. As a result, a bright prospect has been opened that the reliability and reproducibility of diagnosis in Kampo medicine can be significantly improved.

  In addition, for example, in conjunction with the results of the current Kousaosan and Hanka Kokakuto, various Chinese herbal medicines related to stress listed in the specific examples of the embodiments described above (Yokukansan, Yokukansan Kashinhanka, Kamikan Harukasan and By obtaining gene expression results for katsushika ryukotsu oyster bath etc.), by using these herbal medicines as test drugs for examining mental status, the medical field that was impossible in Western medicine, It can be said that the possibility of establishing a new medical method has emerged.

Claims (37)

  A gene, a fragment thereof, or a complementary sequence thereof, which is used for diagnosis of Kampo medicine, characterized in that its expression varies depending on a specific measurement standard of Kampo medicine.   The gene used for diagnosis of Kampo medicine according to claim 1, its fragment, or these, wherein the measurement standard is the appearance / disappearance of a specific “test” evaluated / determined based on Kampo medicine Complementary sequence strand.   The gene used for diagnosis of Chinese medicine, a fragment thereof, or a complementary sequence chain thereof, according to claim 2, wherein the "proof" is a "proof" of a specific Chinese medicine.   The gene, a fragment thereof or a complementary sequence chain thereof used for diagnosis of Kampo medicine according to claim 3, wherein the "proof" of the Chinese medicine is "proof of Koshosan".   The gene used for the diagnosis of Kampo medicine according to claim 3, a fragment thereof, or a complementary sequence chain thereof, wherein the "certificate" of the Chinese medicine is "Proof of Hanatsu Kobokuto".   The gene used for diagnosis of Chinese medicine, a fragment thereof or a complementary sequence chain thereof according to claim 1, wherein the measurement standard is taking a specific Chinese medicine.   The gene used for diagnosis of Chinese medicine, a fragment thereof, or a complementary sequence chain thereof, according to claim 1, wherein the measurement standard is the effectiveness of a specific Chinese medicine.   The gene used for the diagnosis of Kampo medicine according to claim 6 or 7, or a complementary sequence chain thereof, wherein the Kampo medicine is Kasousan.   The gene used for the diagnosis of Kampo medicine according to claim 6 or 7, or a complementary sequence thereof, wherein the Kampo medicine is Hanka Koto. The measurement standard is a combination of at least two of the appearance / disappearance of a specific “certification” evaluated and determined based on Kampo medicine, the taking of a specific Chinese medicine, and the effectiveness of a specific Chinese medicine. The gene used for the diagnosis of the Chinese medicine according to claim 1, a fragment thereof, or a complementary sequence chain thereof. The gene is described in SEQ ID NOs: Hs.487001, Hs.436042, and Hs.61714 in the nucleotide sequence described in SEQ ID NO: 1 to SEQ ID NO: 49 in the sequence listing and the NCBI Unigene database. The gene, its fragment or their complementary sequence chain used for the diagnosis of Kampo medicine according to claim 4 or 8, characterized in that it is any one or more of the genes, its fragments or their complementary sequence strands . The genes are listed in NCBI Unigene database SEQ ID NOs: Hs.180911, Hs.294176, Hs.53250, Hs.282331, Hs.440606, Hs.86538, Hs.61232, Hs.24884, Hs.110299, Hs.303523 , Hs.115617, Hs.3321, Hs.334527, Hs.161640, Hs.225943, Hs.323213, Hs.186582, Hs.24583, Hs.234775, Hs.512633, Hs.377022, Hs.37189, Hs. .262480, Hs.8852, Hs.527699, Hs.445241, Hs.437457, Hs.436628, Hs.115497, Hs.497707, Hs.150826, Hs.266902, Hs.442801, Hs.8022, Hs.432379 , Hs.460471, Hs.434195, Hs.145156, Hs.396566, Hs.367761, Hs.12902, Hs.297997, Hs.183390, Hs.479572, Hs.450425, Hs.283085, Hs.103388, Hs .18213, Hs.378736, Hs.389964, Hs.387258, Hs.355820, Hs.210850, Hs.158318, Hs.157461, Hs.17860, Hs.355711, Hs.74376, Hs.111726, Hs.54468 , Hs.75236, Hs.193228, Hs.512660, Hs.130685, Hs.272493, Hs.414091, Hs.113912, Hs.128204, Hs.115181, Hs.86088, Hs.431156, Hs.115830, Hs .37058, Hs.125507, Hs.376586, Hs.97808, Hs.493514, Hs.412 355, Hs.97933, Hs.288727, Hs.499520, Hs.53996, Hs.144028, Hs.131555, Hs.232779, Hs.382347, Hs.87224, Hs.382870, Hs.82535, Hs.235498, Hs.438482, Hs.129778, Hs.22575, Hs.98967, Hs.320086, Hs.412022, Hs.259498, Hs.445025, Hs.334022, Hs.51120, Hs.302109, Hs.268744, Hs. 152664, Hs.153203, Hs.201269, Hs.448749, Hs.408843, Hs.234790, Hs.147305, Hs.436494, Hs.12840, Hs.158963, Hs.283880, Hs.476396, Hs.148956, Hs.326012, Hs.437137, Hs.406763, Hs.150276, Hs.408195, Hs.496614, Hs.251573, Hs.205457, Hs.132275, Hs.233325, Hs.343666, Hs.49753, Hs. 432815, Hs.443683, Hs.143907, Hs.22116, Hs.336224, Hs.22573, Hs.66, Hs.234774, Hs.130310, Hs.25363, Hs.78465, Hs.367888, Hs.127059, Hs.140821, Hs.511968, Hs.432445, Hs.350765, Hs.48695, Hs.430138, Hs.99607, Hs.213493, Hs.8345, Hs.502577, Hs.57876, Hs.100299, Hs. 458408, Hs.406639, Hs.512760, Hs.123457, Hs.326561, Hs.446702, Hs.89121, Hs.17681, Hs.148074, Hs.97714, Hs.2936, H s.158357, Hs.499247, Hs.153692, Hs.406234, Hs.241385, Hs.271809, Hs.278568, Hs.254122, Hs.318791, Hs.189482, Hs.282938, Hs.390808, Hs. 2149, Hs.423112, Hs.301715, Hs.55016, Hs.99120, Hs.511985, Hs.359978, Hs.204238, Hs.283506, Hs.447877, Hs.137674, Hs.266738, Hs.302145, Hs.399966, Hs.149098, Hs.431010, Hs.222312, Hs.2207, Hs.25094, Hs.79881, Hs.116651, Hs.25328, Hs.501480, Hs.8346, Hs.157796, Hs. 21239, Hs.508901, Hs.144875, Hs.40735, Hs.341806, Hs.125423, Hs.511880, Hs.433827, Hs.307044, Hs.306697, Hs.134807, Hs.387367, Hs.335355, Hs.349109, Hs.418105, Hs.376337, Hs.137574, Hs.44077, Hs.44899, Hs.184945, Hs.114088, Hs.376025, Hs.21258, Hs.207407, Hs.117938, Hs. 169071, Hs.385986, Hs.284153, Hs.95582, Hs.21380, Hs.418083, Hs.91625, Hs.397167, Hs.437046, Hs.14468, Hs.26309, Hs.479080, Hs.352530, Hs.73958, Hs.171025, Hs.121001, Hs.11713, Hs.280311, Hs.8535, Hs.283326, Hs.407474, Hs.451697, Hs.14732, Hs.7389 0, Hs.42650, Hs.7327, Hs.287640, Hs.194659, Hs.191381, Hs.406789, Hs.227098, Hs.7934, Hs.512643, Hs.256583, Hs.408355, Hs.155182, Hs.30340, Hs.178023, Hs.58974, Hs.390568, Hs.185796, Hs.115263, Hs.414251, Hs.119693, Hs.511776, Hs.196530, Hs.157573, Hs.36761, Hs. 223581, Hs.511753, Hs.17170, Hs.448823, Hs.102929, Hs.37616, Hs.511824, Hs.354013, Hs.132380, Hs.371496, Hs.458427, Hs.32967, Hs.143751, Hs.253690, Hs.352, Hs.21177, Hs.406681, Hs.435976, Hs.46679, Hs.148101, Hs.9914, Hs.310230, Hs.376590, Hs.458397, Hs.58414, Hs. 17165, Hs.407015, Hs.470704, Hs.246914, Hs.15420, Hs.436282, Hs.257368, Hs.512739, Hs.387679, Hs.283078, Hs.516782, Hs.109752, Hs.201667, Hs.149504, Hs.398139, Hs.94367, Hs.112208, Hs.395779, Hs.445563, Hs.65135, Hs.187203, Hs.490216, Hs.487918, Hs.436495, Hs.125571, Hs. 519634, Hs.399769, Hs.511987, Hs.26176, Hs.25934, Hs.351825, Hs.283794, Hs.256067, Hs.251699, Hs.436378, Hs.523755, H any one or more of genes described in s.449884, Hs.41154, Hs.54609, Hs.458291, Hs.408336, and Hs.2012, fragments thereof, or complementary sequence chains thereof The gene used for diagnosis of Chinese medicine according to claim 4 or 8, a fragment thereof, or a complementary sequence chain thereof. The genes are listed in NCBI Unigene database SEQ ID NOs: Hs.372133, Hs.281004, 7.59 Hs.420563, Hs.376818, Hs.15780, Hs.493450, Hs.306814, Hs.511810, Hs.143241, Hs. 278924, Hs.512632, Hs.367633, Hs.127363, Hs.414110, Hs.434143, Hs.36567, Hs.356605, Hs.414648, Hs.104888, Hs.197435, Hs.410810, Hs.434375, Hs.56279, Hs.125487, Hs.51011, Hs.170953, Hs.66392, Hs.48372, Hs.356664, Hs.420269, Hs.3017, Hs.306811, Hs.93121, Hs.438880, Hs. 302029, Hs.463017, Hs.412559, Hs.17518, Hs.2133, Hs.400380, Hs.75813, Hs.287656, Hs.334347, Hs.411572, Hs.6496, Hs.268764, Hs.385496, Hs.410088, Hs.24835, Hs.446504, Hs.7972, Hs.292057, Hs.435775, Hs.78106, Hs.217754, Hs.153704, Hs.341906, Hs.250830, Hs.68846, Hs. 438712, Hs.99480, Hs.434418, Hs.171980, Hs.376810, Hs.324335, Hs.37936, Hs.512546, Hs.128836, Hs.406330, Hs.302352, Hs.169825, Hs.522765, Hs.526526, Hs.82002, Hs.526394, Hs.512785, Hs.1937, Hs.10 5700, Hs.122895, Hs.9589, Hs.99439, Hs.334828, Hs.126161, Hs.465202, Hs.310231, Hs.94875, Hs.203557, Hs.444729, Hs.449965, Hs.122309, Hs.190090, Hs.355714, Hs.153952, Hs.408577, Hs.287370, Hs.185202, Hs.29792, Hs.4845, Hs.27183, Hs.486433, Hs.439463, Hs.194665, Hs. 129312, Hs.163682, Hs.73853, Hs.2689, Hs.283900, Hs.162604, Hs.381367, Hs.288218, Hs.203349, Hs.433891, Hs.248065, Hs.440603, Hs.144913, Hs.199316, Hs.221660, Hs.21554, Hs.510244, Hs.436325, Hs.117979, Hs.356888, Hs.306322, Hs.310431, Hs.356501, Hs.444708, Hs.159609, Hs. 18069, Hs.373612, Hs.12802, Hs.508901, Hs.504580, Hs.308242, Hs.128580, Hs.112184, Hs.306298, Hs.343800, Hs.50841, Hs.137516, Hs.413095, Hs.105640, Hs.144696, Hs.175411, Hs.207407, Hs.406856, Hs.122959, Hs.143792, Hs.170195, Hs.381715, Hs.443292, Hs.56874, Hs.147096, Hs. 521267, Hs.196956, Hs.80720, Hs.440953, Hs.352387, Hs.434107, Hs.112720, Hs.155558, Hs.147352, Hs.146329, Hs .463171, Hs.445445, Hs.156892, Hs.212670, Hs.129895, Hs.356076, Hs.439590, Hs.278613, Hs.54697, Hs.388917, Hs.55923, Hs.460377, Hs.416244 , Hs.234786, Hs.129969, Hs.275711, Hs.127842, Hs.302772, Hs.337008, Hs.48480, Hs.121115, Hs.318773, Hs.98564, Hs.276466, Hs.78824, Hs .124502, Hs.325530, Hs.375664, Hs.516311, Hs.367688, Hs.386155, Hs.189174, Hs.159264, Hs.386143, Hs.158446, Hs.209461, Hs.163484, Hs.300559 , Hs.155223, Hs.448979, Hs.368420, Hs.289044, Hs.74088, Hs.93907, Hs.408264, Hs.439168, Hs.437433, Hs.448468, Hs.310194, Hs.45152, Hs .283095, Hs.500957, Hs.431156, Hs.511922, Hs.406549, Hs.288731, Hs.123419, Hs.334702, Hs.114172, Hs.408261, Hs.436657, Hs.125300, Hs.203 , Hs.287499, Hs.102943, Hs.145949, Hs.248290, Hs.418167, Hs.55481, Hs.434181, Hs.275906, Hs.249126, Hs.147256, Hs.178295, Hs.385890, Hs .201800, Hs.96633, Hs.445489, Hs.449650, Hs.102708, Hs.433381, Hs.27263, Hs.512782, Hs.347537, Hs.12411 5, Hs.1270, Hs.349121, Hs.293884, Hs.143943, Hs.12742, Hs.166705, Hs.13209, Hs.77719, Hs.129694, Hs.126906, Hs.151414, Hs.212584, Hs.232819, Hs.41324, Hs.140821, Hs.351593, Hs.12248, Hs.434281, Hs.283506, Hs.66412.18 Hs.2115872.17 Hs.427712.17 Hs.447703, Hs.372208, Hs.379010, Hs .437008, Hs.99311, Hs.464896, Hs.323079, Hs.509439, Hs.440088, Hs.34045, Hs.406073, Hs.455335, Hs.175465, Hs.507549, Hs.127775, Hs.132888 , Hs.8728, Hs.157231, Hs.62863, Hs.169600, Hs.414332, Hs.259403, Hs.101949, Hs.436339, Hs.128668, Hs.380167, Hs.79340, Hs.43125, Hs .2251, Hs.142296, Hs.288155, Hs.399740, Hs.27524, Hs.165464, Hs.123642, Hs.125156, Hs.58561, Hs.418966, Hs.447121, Hs.24734, Hs.348709 , Hs.89436, Hs.2563, Hs.186363, Hs.169470, Hs.6540, Hs.223206, Hs.439169, Hs.133250, Hs.172562, Hs.349705, Hs.405457, Hs.145894, Hs .7360, Hs.163546, Hs.102276, Hs.511781, Hs.283794, Hs.171090, Hs.508459, Hs.276808, Hs.248155, Hs.436042 The Chinese medicine according to claim 4 or 8, which is one or more of the genes described in Hs.371212, Hs.221040 and Hs.374990, a fragment thereof or a complementary sequence chain thereof. A gene used for medical diagnosis, a fragment thereof, or a complementary sequence thereof. The genes are listed in NCBI Unigene database SEQ ID NOs: Hs. 58323, Hs. 160373, Hs. 334832, Hs. 150136, Hs. 272385, Hs. 382870, Hs. 430919, Hs. 387551, Hs. 194408, Hs. 406961 , Hs.164021, Hs.24884, Hs.437211, Hs.436628, Hs.283880, Hs.110299, Hs.390242, Hs.113291, Hs.278894, Hs.90221, Hs.24583, Hs.266902, Hs .148956, Hs.162282, Hs.103388, Hs.406300, Hs.382347, Hs.413430, Hs.449884, Hs.24889, Hs.440606, Hs.36069, Hs.131255, Hs.369557, Hs.512660 , Hs.359981, Hs.283085, Hs.163546, Hs.117938, Hs.74376, Hs.438482, Hs.482392, Hs.320086, Hs.95990, Hs.9469, Hs.108642, Hs.180016, Hs .479572, Hs.169395, Hs.335319, Hs.442801, Hs.111726, Hs.183390, Hs.449407, Hs.434195, Hs.17546, Hs.127951, Hs.458408, Hs.287374, Hs.150826 , Hs.181483, Hs.115467, Hs.232779, Hs.96557, Hs.406722, Hs.201269, Hs.47313, Hs.8852, Hs.283326, Hs.225943, Hs.12902, Hs.500371, Hs .7296, Hs.430854, Hs.278568, Hs.132275, Hs.66392, Hs.4 37179, Hs.8850, Hs.98967, Hs.57876, Hs.459828, Hs.310230, Hs.25328, Hs.301956, Hs.144875, Hs.110457, Hs.509265, Hs.60339, Hs.511880, Hs.446152, Hs.48100, Hs.247975, Hs.479080, Hs.490096, Hs.8346, Hs.131555, Hs.32967, Hs.307003, Hs.127032, Hs.109268, Hs.80720, Hs. 266738, Hs.113912, Hs.175780, Hs.497972, Hs.18213, Hs.152664, Hs.459847, Hs.378736, Hs.458427, Hs.36761, Hs.429531, Hs.149797, Hs.444135, Hs.22575, Hs.272191, Hs.512643, Hs.54713, Hs.820, Hs.77221, Hs.253690, Hs.436282, Hs.181289, Hs.170560, Hs.426734, Hs.334527, Hs. 406701, Hs.177434, Hs.511968, Hs.389964, Hs.251699, Hs.207805, Hs.140821, Hs.281004, Hs.435675, Hs.292597, Hs.178715, Hs.161394, Hs.396332, Hs.302109, Hs.86088, Hs.356794, Hs.449392, Hs.134807, Hs.210850, Hs.18705, Hs.326444, Hs.287453, Hs.130522, Hs.234775, Hs.438555, Hs. 171532, Hs.54617, Hs.268843, Hs.11713, Hs.5025, Hs.288727, Hs.412022, Hs.78465, Hs.423112, Hs.497707, Hs.235390 Hs.33455, Hs.371278, Hs.237642, Hs.234790, Hs.78792, Hs.115181, Hs.115617, Hs.463017, Hs.406789, Hs.378753, Hs.256583, Hs.99430, Hs. 525361, Hs.148559, Hs.249181, Hs.23853, Hs.172052, Hs.90821, Hs.444182, Hs.123474, Hs.284310, Hs.137574, Hs.102929, Hs.511985, Hs.371335, Hs.528103, Hs.355711, Hs.496614, Hs.27621, Hs.183006, Hs.27021, Hs.389571, Hs.227457, Hs.334772, Hs.512760, Hs.53996, Hs.207407, Hs. 297997, Hs.512808, Hs.149467, Hs.510932, Hs.272493, Hs.79881, Hs.3052, Hs.415895, Hs.388220, Hs.253193, Hs.124502, Hs.232803, Hs.269777, Hs.12264, Hs.380763, Hs.147595, Hs.411274, Hs.441481, Hs.376025, Hs.250895, Hs.468922, Hs.161427, Hs.385986, Hs.439760, Hs.313413, Hs. 408843, Hs.58974, Hs.430755, Hs.99120, Hs.25363, Hs.287405, Hs.131035, Hs.335355, Hs.259471, Hs.235888, Hs.144527, Hs.414091, Hs.480174, Hs.445241, Hs.44888, Hs.382683, Hs.439494, Hs.288520, Hs.234027, Hs.130685, Hs.399966, Hs.434486, Hs.407 002, Hs.333958, Hs.238648, Hs.308045, Hs.196470, Hs.144531, Hs.7327, Hs.83916, Hs.169441, Hs.73890, Hs.432374, Hs.143751, Hs.2074, Hs.158278, Hs.158963, Hs.490216, Hs.103422, Hs.133361, Hs.404220, Hs.169071, Hs.288718, Hs.144130, Hs.336224, Hs.112669, Hs.159265, Hs. 122110, Hs.7835, Hs.407015, Hs.441708, Hs.452398, Hs.439124, Hs.443020, Hs.499520, Hs.356572, Hs.284200, Hs.78944, Hs.512387, Hs.235498, Hs.133082, Hs.471423, Hs.73625, Hs.9914, Hs.355862, Hs.82120, Hs.443392, Hs.401954, Hs.436140, Hs.461482, Hs.433596, Hs.434103, Hs. 385898, Hs.348920, Hs.81848, Hs.432379, Hs.79025, Hs.407162, Hs.197922, Hs.180171, Hs.408200, Hs.444529, Hs.164649, Hs.4224, Hs.460471, Hs.149152, Hs.458397, Hs.124211, Hs.415497, Hs.293917, Hs.253489, Hs.14822, Hs.376590, Hs.179112, Hs.152337, Hs.438300, Hs.344099, Hs. 421986, Hs.432458, Hs.351414, Hs.54460, Hs.349227, Hs.47312, Hs.185796, Hs.26981, Hs.267038, Hs.448749, Hs.1701 14, Hs.95582, Hs.352601, Hs.353001, Hs.352617, Hs.414164, Hs.30340, Hs.359978, Hs.298241, Hs.178292, Hs.156232, Hs.406681, Hs.411644, Any of the genes described in Hs.97933, Hs.137674, Hs.476396, Hs.388877, Hs.155573, Hs.87, Hs.153203, Hs.458434, Hs.10056, Hs.148074 and Hs.377022 The gene, a fragment thereof or a complementary sequence chain thereof according to claim 4 or 8, which is one or more genes, a fragment thereof or a complementary sequence strand thereof. The genes are listed in NCBI Unigene database SEQ ID NOs: Hs.436450, Hs.278613, Hs.411239, Hs.445072, Hs.123515, Hs.17518, Hs.128668, Hs.280226, Hs.511758, Hs.233437 , Hs.187802, Hs.61929, Hs.406741, Hs.245431, Hs.20082, Hs.29798, Hs.222, Hs.162089, Hs.37936, Hs.144913, Hs.166351, Hs.313, Hs .522901, Hs.450230, Hs.89679, Hs.323966, Hs.439168, Hs.159629, Hs.186363, Hs.172052, Hs.250723, Hs.356605, Hs.376407, Hs.188021, Hs.386264 , Hs.40510, Hs.408067, Hs.58009, Hs.222909, Hs.113227, Hs.350800, Hs.377070, Hs.178295, Hs.222746, Hs.351811, Hs.440088, Hs.490397, Hs .344400, Hs.361748, Hs.283045, Hs.27258, Hs.25328, Hs.350765, Hs.103665, Hs.194725, Hs.327621, Hs.408629, Hs.411347, Hs.489592, Hs.386236 , Hs.446451, Hs.105115, Hs.45207, Hs.91283, Hs.178499, Hs.444882, Hs.381370, Hs.369994, Hs.93883, Hs.306230, Hs.413924, Hs.504809, Hs .84665, Hs.389724, Hs.211587, Hs.1560, Hs.406733, Hs.3068 12, Hs.348745, Hs.106688, Hs.129895, Hs.388917, Hs.368746, Hs.413525, Hs.166011, Hs.94865, Hs.250472, Hs.434485, Hs.267038, Hs.445426, Hs. 154084, Hs. 23317, Hs. 75360, Hs. 127405, Hs. 155584, Hs. 47504, Hs. 90318, Hs. 128499, Hs. 283082, Hs. 449006, Hs. 309365, Hs. 436602, Hs. 324335, Hs.351816, Hs.306992, Hs.512632, Hs.443260, Hs.348709, Hs.426324, Hs.227656, Hs.435092, Hs.272200, Hs.347537, Hs.271269, Hs.424414, Hs.707, Hs.248204, Hs.168355, Hs.58559, Hs.407817, Hs.447162, Hs.144870, Hs.435398, Hs.146025, Hs.406485, Hs.326559, Hs.258536, Hs. 169825, Hs.1270, Hs.325335, Hs.335877, Hs.421280, Hs.443120, Hs.389638, Hs.124673, Hs.150138, Hs.476618, Hs.438482, Hs.652, Hs.199372, Hs.493952, Hs.371212, Hs.25156, Hs.301956, Hs.511810, Hs.151408, Hs.157144, Hs.159525, Hs.94327, Hs.449791, Hs.320522, Hs.123114, Hs. 446376, Hs.250666, Hs.201554, Hs.351818, Hs.189575, Hs.132270, Hs.36793, Hs.23367, Hs.104613, Hs.254006, Hs.13 3995, Hs.395205, Hs.476835, Hs.513009, Hs.257584, Hs.5462, Hs.96149, Hs.374720, Hs.46791, Hs.127473, Hs.91813, Hs.271640, Hs.379010, Hs.516739, Hs.376657, Hs.121490, Hs.352240, Hs.203557, Hs.228982, Hs.122115, Hs.112928, Hs.436975, Hs.387258, Hs.433197, Hs.159553, Hs. 437, Hs.288911, Hs.440953, Hs.20315, Hs.104859, Hs.38260, Hs.199316, Hs.511791, Hs.282275, Hs.249126, Hs.163484, Hs.297604, Hs.73853, Hs.27335, Hs.26176, Hs.92683, Hs.212584, Hs.372616, Hs.153647, Hs.121115, Hs.87306, Hs.318775, Hs.353114, Hs.167679, Hs.415209, Hs. 55209, Hs.143600, Hs.156007, Hs.444960, Hs.234016, Hs.283011, Hs.512778, Hs.37107, Hs.414332, Hs.164680, Hs.74115, Hs.422120, Hs.104133, Hs.21861, Hs.249184, Hs.375054, Hs.436424, Hs.302754, Hs.297452, Hs.165387, Hs.493450, Hs.306094, Hs.414110, Hs.118057, Hs.840, Hs. 2207, Hs.343154, Hs.445772, Hs.132272, Hs.59892, Hs.380519, Hs.442936, Hs.274548, Hs.72910, Hs.49433, Hs.26663, H s.155814, Hs.212892, Hs.408488, Hs.37125, Hs.375870, Hs.440695, Hs.341906, Hs.370622, Hs.314141, Hs.118631, Hs.115365, Hs.238964, Hs. 331491, Hs.194714, Hs.434375, Hs.127012, Hs.306622, Hs.211298, Hs.264330, Hs.347534, Hs.148767, Hs.41270, Hs.283096, Hs.110637, Hs.76152, Hs.334, Hs.55412, Hs.497743, Hs.276808, Hs.114599, Hs.461085, Hs.282787, Hs.131834, Hs.21838, Hs.492218, Hs.248114, Hs.501321, Hs. 512787, Hs.268700, Hs.313948, Hs.211477, Hs.2053, Hs.21388, Hs.507546, Hs.1501, Hs.248139, Hs.283779, Hs.511992, Hs.406984, Hs.145680, Hs.403836, Hs.287656, Hs.53263, Hs.257541, Hs.413265, Hs.333742, Hs.156346, Hs.441287, Hs.131101, Hs.436983, Hs.256036, Hs.420036, Hs. 440387, Hs.122309, Hs.479817, Hs.195614, Hs.355584, Hs.65248, Hs.512782, Hs.363315, Hs.43107, Hs.233950, Hs.445445, Hs.82316, Hs.148990, Any one or more of the genes described in Hs.158798, Hs.189067 and Hs.90797, Fragment or gene, fragment or complementary sequence strand of the use in the diagnosis of Kampo medicine according to claim 4 or 8, characterized in that the complementary sequences strand of. The gene is any one or more of a base sequence described in SEQ ID NO: 290 to SEQ ID NO: 338 in the sequence listing or a gene containing a part thereof, a fragment thereof, or a complementary sequence chain thereof. A gene, a fragment thereof, or a complementary sequence chain thereof used for the diagnosis of Kampo medicine according to claim 5 or 9.   A substrate for gene expression analysis, wherein a plurality of the genes according to any one of claims 1, 2, 3, 6 and 10 or fragments thereof or complementary sequence chains thereof are immobilized on a support. .   The genes, fragments thereof or their complementary sequence strands are comprehensively analyzed using a fluorescent differential display method or a DNA microarray method, and at least five genes, their fragments or their complements whose expression changes are recognized. The substrate for gene expression analysis according to claim 17, which is a sequence chain.   A gene expression analysis method using the gene expression analysis platform according to claim 17 or 18. A method for evaluating the “proof” of Kampo medicine using the gene according to any one of claims 1, 2, 3, 6 and 10 or a fragment thereof or a complementary sequence thereof as a target gene. ,
By measuring the expression state of the target gene in the measurement sample obtained from the subject, and comparing the measurement result with the expression state of the target gene in the comparative sample serving as an evaluation standard, An evaluation method for “certification” in Kampo medicine, characterized by evaluating the presence or absence of medical treatment.   21. The expression of the target gene varies depending on the appearance and disappearance of the “proof of Kosyosan” and / or the effectiveness of Kosyosan. Evaluation method of “certification” in Kampo medicine. The target gene is described in SEQ ID NOS: Hs.487001, Hs.436042 and Hs.61714 in the gene including the nucleotide sequence described in SEQ ID NO: 1 to SEQ ID NO: 49 in the sequence listing or a part thereof, and NCBI Unigene database. The method for evaluating "proof" in Chinese medicine according to claim 21, wherein the gene is any one or more of these genes, a fragment thereof, or a complementary sequence chain thereof. The target genes are listed in NCBI Unigene database SEQ ID NOs: Hs.180911, Hs.294176, Hs.53250, Hs.282331, Hs.440606, Hs.86538, Hs.61232, Hs.24884, Hs.110299, Hs. 303523, Hs.115617, Hs.3321, Hs.334527, Hs.161640, Hs.225943, Hs.323213, Hs.186582, Hs.24583, Hs.234775, Hs.512633, Hs.377022, Hs.29189, Hs.262480, Hs.8852, Hs.527699, Hs.445241, Hs.437457, Hs.436628, Hs.115497, Hs.497707, Hs.150826, Hs.266902, Hs.442801, Hs.8022, Hs. 432379, Hs.460471, Hs.434195, Hs.145156, Hs.396566, Hs.367761, Hs.12902, Hs.297997, Hs.183390, Hs.479572, Hs.450425, Hs.283085, Hs.103388, Hs.18213, Hs.378736, Hs.389964, Hs.387258, Hs.355820, Hs.210850, Hs.158318, Hs.157461, Hs.17860, Hs.355711, Hs.74376, Hs.111726, Hs. 54468, Hs.75236, Hs.193228, Hs.512660, Hs.130685, Hs.272493, Hs.414091, Hs.113912, Hs.128204, Hs.115181, Hs.86088, Hs.431156, Hs.115830, Hs.37058, Hs.125507, Hs.376586, Hs.97808, Hs.493514, Hs.412355, Hs.97933, Hs.288727, Hs.499520, Hs.53996, Hs.144028, Hs.131555, Hs.232779, Hs.382347, Hs.87224, Hs.382870, Hs.82535, Hs. 235498, Hs.438482, Hs.129778, Hs.22575, Hs.98967, Hs.320086, Hs.412022, Hs.259498, Hs.445025, Hs.334022, Hs.51120, Hs.302109, Hs.268744, Hs.152664, Hs.153203, Hs.201269, Hs.448749, Hs.408843, Hs.234790, Hs.147305, Hs.436494, Hs.12840, Hs.158963, Hs.283880, Hs.476396, Hs. 148956, Hs.326012, Hs.437137, Hs.406763, Hs.150276, Hs.408195, Hs.496614, Hs.251573, Hs.205457, Hs.132275, Hs.233325, Hs.343666, Hs.49753, Hs.432815, Hs.443683, Hs.143907, Hs.22116, Hs.336224, Hs.22573, Hs.66, Hs.234774, Hs.130310, Hs.25363, Hs.78465, Hs.367888, Hs. 127059, Hs.140821, Hs.511968, Hs.432445, Hs.350765, Hs.48695, Hs.430138, Hs.99607, Hs.213493, Hs.8345, Hs.502577, Hs.57876, Hs.100299, Hs.458408, Hs.406639, Hs.512760, Hs.123457, Hs.326561, Hs.446702, Hs.89121, Hs.17681, Hs.148074, Hs.97714, Hs.29 36, Hs.158357, Hs.499247, Hs.153692, Hs.406234, Hs.241385, Hs.271809, Hs.278568, Hs.254122, Hs.318791, Hs.189482, Hs.282938, Hs.390808, Hs.2149, Hs.423112, Hs.301715, Hs.55016, Hs.99120, Hs.511985, Hs.359978, Hs.204238, Hs.283506, Hs.447877, Hs.137674, Hs.266738, Hs. 302145, Hs.399966, Hs.149098, Hs.431010, Hs.222312, Hs.2207, Hs.25094, Hs.79881, Hs.116651, Hs.25328, Hs.501480, Hs.8346, Hs.157796, Hs.21239, Hs.508901, Hs.144875, Hs.40735, Hs.341806, Hs.125423, Hs.511880, Hs.433827, Hs.307044, Hs.306697, Hs.134807, Hs.387367, Hs. 335355, Hs.349109, Hs.418105, Hs.376337, Hs.137574, Hs.44077, Hs.44899, Hs.184945, Hs.114088, Hs.376025, Hs.21258, Hs.207407, Hs.117938, Hs.169071, Hs.385986, Hs.284153, Hs.95582, Hs.21380, Hs.418083, Hs.91625, Hs.397167, Hs.437046, Hs.14468, Hs.26309, Hs.479080, Hs. 352530, Hs.73958, Hs.171025, Hs.121001, Hs.11713, Hs.280311, Hs.8535, Hs.283326, Hs.407474, Hs.451697, Hs.14732, H s.73890, Hs.42650, Hs.7327, Hs.287640, Hs.194659, Hs.191381, Hs.406789, Hs.227098, Hs.7934, Hs.512643, Hs.256583, Hs.408355, Hs. 155182, Hs.30340, Hs.178023, Hs.58974, Hs.390568, Hs.185796, Hs.115263, Hs.414251, Hs.119693, Hs.511776, Hs.196530, Hs.157573, Hs.36761, Hs.223581, Hs.511753, Hs.17170, Hs.448823, Hs.102929, Hs.37616, Hs.511824, Hs.354013, Hs.132380, Hs.371496, Hs.458427, Hs.32967, Hs. 143751, Hs.253690, Hs.352, Hs.21177, Hs.406681, Hs.435976, Hs.46679, Hs.148101, Hs.9914, Hs.310230, Hs.376590, Hs.458397, Hs.58414, Hs.17165, Hs.407015, Hs.470704, Hs.246914, Hs.15420, Hs.436282, Hs.257368, Hs.512739, Hs.387679, Hs.283078, Hs.516782, Hs.109752, Hs.109752, Hs. 201667, Hs.149504, Hs.398139, Hs.94367, Hs.112208, Hs.395779, Hs.445563, Hs.65135, Hs.187203, Hs.490216, Hs.487918, Hs.436495, Hs.125571, Hs.519634, Hs.399769, Hs.511987, Hs.26176, Hs.25934, Hs.351825, Hs.283794, Hs.256067, Hs.251699, Hs.436378, Hs.5237 55, Hs.449884, Hs.41154, Hs.54609, Hs.458291, Hs.408336 and Hs.2012 are one or more genes, fragments thereof, or complementary sequence strands thereof. The method for evaluating “certification” in Chinese medicine according to claim 21. The target gene is represented by SEQ ID NOs: Hs.372133, Hs.281004, 7.59 Hs.420563, Hs.376818, Hs.15780, Hs.493450, Hs.306814, Hs.511810, Hs.143241, Hs in the NCBI Unigene database. .278924, Hs.512632, Hs.367633, Hs.127363, Hs.414110, Hs.434143, Hs.36567, Hs.356605, Hs.414648, Hs.104888, Hs.197435, Hs.410810, Hs.434375 , Hs.56279, Hs.125487, Hs.51011, Hs.170953, Hs.66392, Hs.48372, Hs.356664, Hs.420269, Hs.3017, Hs.306811, Hs.93121, Hs.438880, Hs .302029, Hs.463017, Hs.412559, Hs.17518, Hs.2133, Hs.400380, Hs.75813, Hs.287656, Hs.334347, Hs.411572, Hs.6496, Hs.268764, Hs.385496 , Hs.410088, Hs.24835, Hs.446504, Hs.7972, Hs.292057, Hs.435775, Hs.78106, Hs.217754, Hs.153704, Hs.341906, Hs.250830, Hs.68846, Hs .438712, Hs.99480, Hs.434418, Hs.171980, Hs.376810, Hs.324335, Hs.37936, Hs.512546, Hs.128836, Hs.406330, Hs.302352, Hs.169825, Hs.522765 , Hs.526526, Hs.82002, Hs.526394, Hs.512785, Hs.1937 Hs.105700, Hs.122895, Hs.9589, Hs.99439, Hs.334828, Hs.126161, Hs.465202, Hs.310231, Hs.94875, Hs.203557, Hs.444729, Hs.449965, Hs. 122309, Hs.190090, Hs.355714, Hs.153952, Hs.408577, Hs.287370, Hs.185202, Hs.29792, Hs.4845, Hs.27183, Hs.486433, Hs.439463, Hs.194665, Hs.129312, Hs.163682, Hs.73853, Hs.2689, Hs.283900, Hs.162604, Hs.381367, Hs.288218, Hs.203349, Hs.433891, Hs.248065, Hs.440603, Hs. 144913, Hs.199316, Hs.221660, Hs.21554, Hs.510244, Hs.436325, Hs.117979, Hs.356888, Hs.306322, Hs.310431, Hs.356501, Hs.444708, Hs.159609, Hs.18069, Hs.373612, Hs.12802, Hs.508901, Hs.504580, Hs.308242, Hs.128580, Hs.112184, Hs.306298, Hs.343800, Hs.50841, Hs.137516, Hs. 413095, Hs.105640, Hs.144696, Hs.175411, Hs.207407, Hs.406856, Hs.122959, Hs.143792, Hs.170195, Hs.381715, Hs.443292, Hs.56874, Hs.147096, Hs.521267, Hs.196956, Hs.80720, Hs.440953, Hs.352387, Hs.434107, Hs.112720, Hs.155558, Hs.147352, Hs.14632 9, Hs.463171, Hs.445445, Hs.156892, Hs.212670, Hs.129895, Hs.356076, Hs.439590, Hs.278613, Hs.54697, Hs.388917, Hs.55923, Hs.460377, Hs.416244, Hs.234786, Hs.129969, Hs.275711, Hs.127842, Hs.302772, Hs.337008, Hs.48480, Hs.121115, Hs.318773, Hs.98564, Hs.276466, Hs. 78824, Hs.124502, Hs.325530, Hs.375664, Hs.516311, Hs.367688, Hs.386155, Hs.189174, Hs.159264, Hs.386143, Hs.158446, Hs.209461, Hs.163484, Hs.300559, Hs.155223, Hs.448979, Hs.368420, Hs.289044, Hs.74088, Hs.93907, Hs.408264, Hs.439168, Hs.437433, Hs.448468, Hs.310194, Hs. 45152, Hs.283095, Hs.500957, Hs.431156, Hs.511922, Hs.406549, Hs.288731, Hs.123419, Hs.334702, Hs.114172, Hs.408261, Hs.436657, Hs.125300, Hs.203, Hs.287499, Hs.102943, Hs.145949, Hs.248290, Hs.418167, Hs.55481, Hs.434181, Hs.275906, Hs.249126, Hs.147256, Hs.178295, Hs. 385890, Hs.201800, Hs.96633, Hs.445489, Hs.449650, Hs.102708, Hs.433381, Hs.27263, Hs.512782, Hs.347537, Hs .124115, Hs.1270, Hs.349121, Hs.293884, Hs.143943, Hs.12742, Hs.166705, Hs.13209, Hs.77719, Hs.129694, Hs.126906, Hs.151414, Hs.212584 , Hs.232819, Hs.41324, Hs.140821, Hs.351593, Hs.12248, Hs.434281, Hs.283506, Hs.66412.18 Hs.2115872.17 Hs.427712.17 Hs.447703, Hs.372208, Hs.379010, Hs.437008, Hs.99311, Hs.464896, Hs.323079, Hs.509439, Hs.440088, Hs.34045, Hs.406073, Hs.455335, Hs.175465, Hs.507549, Hs.127775, Hs. 132888, Hs.8728, Hs.157231, Hs.62863, Hs.169600, Hs.414332, Hs.259403, Hs.101949, Hs.436339, Hs.128668, Hs.380167, Hs.79340, Hs.43125, Hs.2251, Hs.142296, Hs.288155, Hs.399740, Hs.27524, Hs.165464, Hs.123642, Hs.125156, Hs.58561, Hs.418966, Hs.447121, Hs.24734, Hs. 348709, Hs.89436, Hs.2563, Hs.186363, Hs.169470, Hs.6540, Hs.223206, Hs.439169, Hs.133250, Hs.172562, Hs.349705, Hs.405457, Hs.145894, Hs.7360, Hs.163546, Hs.102276, Hs.511781, Hs.283794, Hs.171090, Hs.508459, Hs.276808, Hs.248155, Hs.43 The Chinese medicine according to claim 21, which is one or more of the genes described in 6042, Hs.371212, Hs.221040 and Hs.374990, a fragment thereof or a complementary sequence chain thereof. Evaluation method of “proof” in medical care. The target genes are listed in NCBI Unigene database SEQ ID NOs: Hs. 58323, Hs. 160373, Hs. 334832, Hs. 150136, Hs. 272385, Hs. 382870, Hs. 430919, Hs. 387551, Hs. 194408, Hs. 406961, Hs.164021, Hs.24884, Hs.437211, Hs.436628, Hs.283880, Hs.110299, Hs.390242, Hs.113291, Hs.278894, Hs.90221, Hs.24583, Hs.266902, Hs.148956, Hs.162282, Hs.103388, Hs.406300, Hs.382347, Hs.413430, Hs.449884, Hs.24889, Hs.440606, Hs.36069, Hs.131255, Hs.369557, Hs. 512660, Hs.359981, Hs.283085, Hs.163546, Hs.117938, Hs.74376, Hs.438482, Hs.482392, Hs.320086, Hs.95990, Hs.9469, Hs.108642, Hs.180016, Hs.479572, Hs.169395, Hs.335319, Hs.442801, Hs.111726, Hs.183390, Hs.449407, Hs.434195, Hs.17546, Hs.127951, Hs.458408, Hs.287374, Hs. 150826, Hs.181483, Hs.115467, Hs.232779, Hs.96557, Hs.406722, Hs.201269, Hs.47313, Hs.8852, Hs.283326, Hs.225943, Hs.12902, Hs.500371, Hs.7296, Hs.430854, Hs.278568, Hs.132275, Hs.66392 Hs.437179, Hs.8850, Hs.98967, Hs.57876, Hs.459828, Hs.310230, Hs.25328, Hs.301956, Hs.144875, Hs.110457, Hs.509265, Hs.60339, Hs. 511880, Hs.446152, Hs.48100, Hs.247975, Hs.479080, Hs.490096, Hs.8346, Hs.131555, Hs.32967, Hs.307003, Hs.127032, Hs.109268, Hs.80720, Hs.266738, Hs.113912, Hs.175780, Hs.497972, Hs.18213, Hs.152664, Hs.459847, Hs.378736, Hs.458427, Hs.36761, Hs.429531, Hs.149797, Hs. 444135, Hs.22575, Hs.272191, Hs.512643, Hs.54713, Hs.820, Hs.77221, Hs.253690, Hs.436282, Hs.181289, Hs.170560, Hs.426734, Hs.334527, Hs.406701, Hs.177434, Hs.511968, Hs.389964, Hs.251699, Hs.207805, Hs.140821, Hs.281004, Hs.435675, Hs.292597, Hs.178715, Hs.161394, Hs. 396332, Hs.302109, Hs.86088, Hs.356794, Hs.449392, Hs.134807, Hs.210850, Hs.18705, Hs.326444, Hs.287453, Hs.130522, Hs.234775, Hs.438555, Hs.171532, Hs.54617, Hs.268843, Hs.11713, Hs.5025, Hs.288727, Hs.412022, Hs.78465, Hs.423112, Hs.497707, Hs.2 35390, Hs.33455, Hs.371278, Hs.237642, Hs.234790, Hs.78792, Hs.115181, Hs.115617, Hs.463017, Hs.406789, Hs.378753, Hs.256583, Hs.99430, Hs.525361, Hs.148559, Hs.249181, Hs.23853, Hs.172052, Hs.90821, Hs.444182, Hs.123474, Hs.284310, Hs.137574, Hs.102929, Hs.511985, Hs. 371335, Hs.528103, Hs.355711, Hs.496614, Hs.27621, Hs.183006, Hs.27021, Hs.389571, Hs.227457, Hs.334772, Hs.512760, Hs.53996, Hs.207407, Hs.297997, Hs.512808, Hs.149467, Hs.510932, Hs.272493, Hs.79881, Hs.3052, Hs.415895, Hs.388220, Hs.253193, Hs.124502, Hs.232803, Hs. 269777, Hs.12264, Hs.380763, Hs.147595, Hs.411274, Hs.441481, Hs.376025, Hs.250895, Hs.468922, Hs.161427, Hs.385986, Hs.439760, Hs.313413, Hs.408843, Hs.58974, Hs.430755, Hs.99120, Hs.25363, Hs.287405, Hs.131035, Hs.335355, Hs.259471, Hs.235888, Hs.144527, Hs.414091, Hs. 480174, Hs.445241, Hs.44888, Hs.382683, Hs.439494, Hs.288520, Hs.234027, Hs.130685, Hs.399966, Hs.434486, Hs.407002, Hs.333958, Hs.238648, Hs.308045, Hs.196470, Hs.144531, Hs.7327, Hs.83916, Hs.169441, Hs.73890, Hs.432374, Hs.143751, Hs. 2074, Hs.158278, Hs.158963, Hs.490216, Hs.103422, Hs.133361, Hs.404220, Hs.169071, Hs.288718, Hs.144130, Hs.336224, Hs.112669, Hs.159265, Hs.122110, Hs.7835, Hs.407015, Hs.441708, Hs.452398, Hs.439124, Hs.443020, Hs.499520, Hs.356572, Hs.284200, Hs.78944, Hs.512387, Hs. 235498, Hs.133082, Hs.471423, Hs.73625, Hs.9914, Hs.355862, Hs.82120, Hs.443392, Hs.401954, Hs.436140, Hs.461482, Hs.433596, Hs.434103, Hs.385898, Hs.348920, Hs.81848, Hs.432379, Hs.79025, Hs.407162, Hs.197922, Hs.180171, Hs.408200, Hs.444529, Hs.164649, Hs.4224, Hs. 460471, Hs.149152, Hs.458397, Hs.124211, Hs.415497, Hs.293917, Hs.253489, Hs.14822, Hs.376590, Hs.179112, Hs.152337, Hs.438300, Hs.344099, Hs.421986, Hs.432458, Hs.351414, Hs.54460, Hs.349227, Hs.47312, Hs.185796, Hs.26981, Hs.267038, Hs.448749, H s.170114, Hs.95582, Hs.352601, Hs.353001, Hs.352617, Hs.414164, Hs.30340, Hs.359978, Hs.298241, Hs.178292, Hs.156232, Hs.406681, Hs. 411644, Hs.97933, Hs.137674, Hs.476396, Hs.388877, Hs.155573, Hs.87, Hs.153203, Hs.458434, Hs.10056, Hs.148074 and Hs.377022 The method for evaluating "proof" in Chinese medicine according to claim 21, wherein the gene is any one or more of these, a fragment thereof, or a complementary sequence chain thereof. The target genes are listed in NCBI Unigene database SEQ ID NOs: Hs.436450, Hs.278613, Hs.411239, Hs.445072, Hs.123515, Hs.17518, Hs.128668, Hs.280226, Hs.511758, Hs. 233437, Hs.187802, Hs.61929, Hs.406741, Hs.245431, Hs.20082, Hs.29798, Hs.222, Hs.162089, Hs.37936, Hs.144913, Hs.166351, Hs.313, Hs.522901, Hs.450230, Hs.89679, Hs.323966, Hs.439168, Hs.159629, Hs.186363, Hs.172052, Hs.250723, Hs.356605, Hs.376407, Hs.188021, Hs. 386264, Hs.40510, Hs.408067, Hs.58009, Hs.222909, Hs.113227, Hs.350800, Hs.377070, Hs.178295, Hs.222746, Hs.351811, Hs.440088, Hs.490397, Hs.344400, Hs.361748, Hs.283045, Hs.27258, Hs.25328, Hs.350765, Hs.103665, Hs.194725, Hs.327621, Hs.408629, Hs.411347, Hs.489592, Hs. 386236, Hs.446451, Hs.105115, Hs.45207, Hs.91283, Hs.178499, Hs.444882, Hs.381370, Hs.369994, Hs.93883, Hs.306230, Hs.413924, Hs.504809, Hs.84665, Hs.389724, Hs.211587, Hs.1560, Hs.406733, H s.306812, Hs.348745, Hs.106688, Hs.129895, Hs.388917, Hs.368746, Hs.413525, Hs.166011, Hs.94865, Hs.250472, Hs.434485, Hs.267038, Hs. 445426, Hs.154084, Hs.23317, Hs.75360, Hs.127405, Hs.155584, Hs.47504, Hs.90318, Hs.128499, Hs.283082, Hs.449006, Hs.309365, Hs.436602, Hs.324335, Hs.351816, Hs.306992, Hs.512632, Hs.443260, Hs.348709, Hs.426324, Hs.227656, Hs.435092, Hs.272200, Hs.347537, Hs.271269, Hs. 424414, Hs.707, Hs.248204, Hs.168355, Hs.58559, Hs.407817, Hs.447162, Hs.144870, Hs.435398, Hs.146025, Hs.406485, Hs.326559, Hs.258536, Hs.169825, Hs.1270, Hs.325335, Hs.335877, Hs.421280, Hs.443120, Hs.389638, Hs.124673, Hs.150138, Hs.476618, Hs.438482, Hs.652, Hs. 199372, Hs.493952, Hs.371212, Hs.25156, Hs.301956, Hs.511810, Hs.151408, Hs.157144, Hs.159525, Hs.94327, Hs.449791, Hs.320522, Hs.123114, Hs.446376, Hs.250666, Hs.201554, Hs.351818, Hs.189575, Hs.132270, Hs.36793, Hs.23367, Hs.104613, Hs.254006 Hs.133995, Hs.395205, Hs.476835, Hs.513009, Hs.257584, Hs.5462, Hs.96149, Hs.374720, Hs.46791, Hs.127473, Hs.91813, Hs.271640, Hs. 379010, Hs.516739, Hs.376657, Hs.121490, Hs.352240, Hs.203557, Hs.228982, Hs.122115, Hs.112928, Hs.436975, Hs.387258, Hs.433197, Hs.159553, Hs.437, Hs.288911, Hs.440953, Hs.20315, Hs.104859, Hs.38260, Hs.199316, Hs.511791, Hs.282275, Hs.249126, Hs.163484, Hs.297604, Hs. 73853, Hs.27335, Hs.26176, Hs.92683, Hs.212584, Hs.372616, Hs.153647, Hs.121115, Hs.87306, Hs.318775, Hs.353114, Hs.167679, Hs.415209, Hs.55209, Hs.143600, Hs.156007, Hs.444960, Hs.234016, Hs.283011, Hs.512778, Hs.37107, Hs.414332, Hs.164680, Hs.74115, Hs.422120, Hs. 104133, Hs.21861, Hs.249184, Hs.375054, Hs.436424, Hs.302754, Hs.297452, Hs.165387, Hs.493450, Hs.306094, Hs.414110, Hs.118057, Hs.840, Hs.2207, Hs.343154, Hs.445772, Hs.132272, Hs.59892, Hs.380519, Hs.442936, Hs.274548, Hs.72910, Hs.49433, Hs.266 63, Hs.155814, Hs.212892, Hs.408488, Hs.37125, Hs.375870, Hs.440695, Hs.341906, Hs.370622, Hs.314141, Hs.118631, Hs.115365, Hs.238964, Hs.331491, Hs.194714, Hs.434375, Hs.127012, Hs.306622, Hs.211298, Hs.264330, Hs.347534, Hs.148767, Hs.41270, Hs.283096, Hs.110637, Hs. 76152, Hs.334, Hs.55412, Hs.497743, Hs.276808, Hs.114599, Hs.461085, Hs.282787, Hs.131834, Hs.21838, Hs.492218, Hs.248114, Hs.501321, Hs.512787, Hs.268700, Hs.313948, Hs.211477, Hs.2053, Hs.21388, Hs.507546, Hs.1501, Hs.248139, Hs.283779, Hs.511992, Hs.406984, Hs. 145680, Hs.403836, Hs.287656, Hs.53263, Hs.257541, Hs.413265, Hs.333742, Hs.156346, Hs.441287, Hs.131101, Hs.436983, Hs.256036, Hs.420036, Hs.440387, Hs.122309, Hs.479817, Hs.195614, Hs.355584, Hs.65248, Hs.512782, Hs.363315, Hs.43107, Hs.233950, Hs.445445, Hs.82316, Hs. Inheritance of any one or more of the genes described in 148990, Hs.158798, Hs.189067 and Hs.90797 The evaluation method of "witness" in Chinese medicine according to claim 21 which is a fragment or complementary sequence strand thereof.   21. The expression of the target gene varies depending on the appearance and disappearance of “Senka Koboku-to”, the use of Hanka-Koboku and / or the effectiveness of Hanka-koboku-to. Evaluation method of “certification” in Kampo medicine. The target gene is any one or more genes, fragments thereof, or their complementary sequence chains of the base sequence described in SEQ ID NO: 290 to SEQ ID NO: 338 of the sequence listing or a part thereof. 28. The method for evaluating "certification" in Chinese medicine according to claim 27. A method for performing an adaptive diagnosis of Kampo medicine in Kampo medicine using the gene according to any one of claims 1, 2, 3, 6, and 10 or a fragment thereof or a complementary sequence thereof as a target gene. ,
By measuring the expression state of the target gene in the measurement sample obtained from the subject, and comparing the measurement result with the expression state of the target gene in the comparative sample serving as an evaluation standard, An adaptive diagnosis method for Kampo medicine in Kampo medicine, wherein the presence or absence of a pattern is observed and the health condition of a subject is evaluated and determined.   The Chinese herbal medicine is Kosousan, and the target gene changes with the appearance / disappearance of “proof of Kosousan”, taking and / or ease of use of Kosousan. 30. The method for adaptive diagnosis of Chinese medicine in Chinese medicine according to claim 29. The target gene is described in SEQ ID NOS: Hs.487001, Hs.436042 and Hs.61714 in the gene including the nucleotide sequence described in SEQ ID NO: 1 to SEQ ID NO: 49 in the sequence listing or a part thereof, and NCBI Unigene database. The method for adaptive diagnosis of Kampo medicine in Kampo medicine according to Claim 30, wherein the gene is any one or more of these genes, a fragment thereof, or a complementary sequence chain thereof. The target genes are listed in NCBI Unigene database SEQ ID NOs: Hs.180911, Hs.294176, Hs.53250, Hs.282331, Hs.440606, Hs.86538, Hs.61232, Hs.24884, Hs.110299, Hs. 303523, Hs.115617, Hs.3321, Hs.334527, Hs.161640, Hs.225943, Hs.323213, Hs.186582, Hs.24583, Hs.234775, Hs.512633, Hs.377022, Hs.29189, Hs.262480, Hs.8852, Hs.527699, Hs.445241, Hs.437457, Hs.436628, Hs.115497, Hs.497707, Hs.150826, Hs.266902, Hs.442801, Hs.8022, Hs. 432379, Hs.460471, Hs.434195, Hs.145156, Hs.396566, Hs.367761, Hs.12902, Hs.297997, Hs.183390, Hs.479572, Hs.450425, Hs.283085, Hs.103388, Hs.18213, Hs.378736, Hs.389964, Hs.387258, Hs.355820, Hs.210850, Hs.158318, Hs.157461, Hs.17860, Hs.355711, Hs.74376, Hs.111726, Hs. 54468, Hs.75236, Hs.193228, Hs.512660, Hs.130685, Hs.272493, Hs.414091, Hs.113912, Hs.128204, Hs.115181, Hs.86088, Hs.431156, Hs.115830, Hs.37058, Hs.125507, Hs.376586, Hs.97808, Hs.493514, Hs.412355, Hs.97933, Hs.288727, Hs.499520, Hs.53996, Hs.144028, Hs.131555, Hs.232779, Hs.382347, Hs.87224, Hs.382870, Hs.82535, Hs. 235498, Hs.438482, Hs.129778, Hs.22575, Hs.98967, Hs.320086, Hs.412022, Hs.259498, Hs.445025, Hs.334022, Hs.51120, Hs.302109, Hs.268744, Hs.152664, Hs.153203, Hs.201269, Hs.448749, Hs.408843, Hs.234790, Hs.147305, Hs.436494, Hs.12840, Hs.158963, Hs.283880, Hs.476396, Hs. 148956, Hs.326012, Hs.437137, Hs.406763, Hs.150276, Hs.408195, Hs.496614, Hs.251573, Hs.205457, Hs.132275, Hs.233325, Hs.343666, Hs.49753, Hs.432815, Hs.443683, Hs.143907, Hs.22116, Hs.336224, Hs.22573, Hs.66, Hs.234774, Hs.130310, Hs.25363, Hs.78465, Hs.367888, Hs. 127059, Hs.140821, Hs.511968, Hs.432445, Hs.350765, Hs.48695, Hs.430138, Hs.99607, Hs.213493, Hs.8345, Hs.502577, Hs.57876, Hs.100299, Hs.458408, Hs.406639, Hs.512760, Hs.123457, Hs.326561, Hs.446702, Hs.89121, Hs.17681, Hs.148074, Hs.97714, Hs.29 36, Hs.158357, Hs.499247, Hs.153692, Hs.406234, Hs.241385, Hs.271809, Hs.278568, Hs.254122, Hs.318791, Hs.189482, Hs.282938, Hs.390808, Hs.2149, Hs.423112, Hs.301715, Hs.55016, Hs.99120, Hs.511985, Hs.359978, Hs.204238, Hs.283506, Hs.447877, Hs.137674, Hs.266738, Hs. 302145, Hs.399966, Hs.149098, Hs.431010, Hs.222312, Hs.2207, Hs.25094, Hs.79881, Hs.116651, Hs.25328, Hs.501480, Hs.8346, Hs.157796, Hs.21239, Hs.508901, Hs.144875, Hs.40735, Hs.341806, Hs.125423, Hs.511880, Hs.433827, Hs.307044, Hs.306697, Hs.134807, Hs.387367, Hs. 335355, Hs.349109, Hs.418105, Hs.376337, Hs.137574, Hs.44077, Hs.44899, Hs.184945, Hs.114088, Hs.376025, Hs.21258, Hs.207407, Hs.117938, Hs.169071, Hs.385986, Hs.284153, Hs.95582, Hs.21380, Hs.418083, Hs.91625, Hs.397167, Hs.437046, Hs.14468, Hs.26309, Hs.479080, Hs. 352530, Hs.73958, Hs.171025, Hs.121001, Hs.11713, Hs.280311, Hs.8535, Hs.283326, Hs.407474, Hs.451697, Hs.14732, H s.73890, Hs.42650, Hs.7327, Hs.287640, Hs.194659, Hs.191381, Hs.406789, Hs.227098, Hs.7934, Hs.512643, Hs.256583, Hs.408355, Hs. 155182, Hs.30340, Hs.178023, Hs.58974, Hs.390568, Hs.185796, Hs.115263, Hs.414251, Hs.119693, Hs.511776, Hs.196530, Hs.157573, Hs.36761, Hs.223581, Hs.511753, Hs.17170, Hs.448823, Hs.102929, Hs.37616, Hs.511824, Hs.354013, Hs.132380, Hs.371496, Hs.458427, Hs.32967, Hs. 143751, Hs.253690, Hs.352, Hs.21177, Hs.406681, Hs.435976, Hs.46679, Hs.148101, Hs.9914, Hs.310230, Hs.376590, Hs.458397, Hs.58414, Hs.17165, Hs.407015, Hs.470704, Hs.246914, Hs.15420, Hs.436282, Hs.257368, Hs.512739, Hs.387679, Hs.283078, Hs.516782, Hs.109752, Hs.109752, Hs. 201667, Hs.149504, Hs.398139, Hs.94367, Hs.112208, Hs.395779, Hs.445563, Hs.65135, Hs.187203, Hs.490216, Hs.487918, Hs.436495, Hs.125571, Hs.519634, Hs.399769, Hs.511987, Hs.26176, Hs.25934, Hs.351825, Hs.283794, Hs.256067, Hs.251699, Hs.436378, Hs.5237 55, Hs.449884, Hs.41154, Hs.54609, Hs.458291, Hs.408336 and Hs.2012 are one or more genes, fragments thereof, or complementary sequence strands thereof. The method for adaptive diagnosis of Chinese medicine in Chinese medicine according to claim 30. The target gene is represented by SEQ ID NOs: Hs.372133, Hs.281004, 7.59 Hs.420563, Hs.376818, Hs.15780, Hs.493450, Hs.306814, Hs.511810, Hs.143241, Hs in the NCBI Unigene database. .278924, Hs.512632, Hs.367633, Hs.127363, Hs.414110, Hs.434143, Hs.36567, Hs.356605, Hs.414648, Hs.104888, Hs.197435, Hs.410810, Hs.434375 , Hs.56279, Hs.125487, Hs.51011, Hs.170953, Hs.66392, Hs.48372, Hs.356664, Hs.420269, Hs.3017, Hs.306811, Hs.93121, Hs.438880, Hs .302029, Hs.463017, Hs.412559, Hs.17518, Hs.2133, Hs.400380, Hs.75813, Hs.287656, Hs.334347, Hs.411572, Hs.6496, Hs.268764, Hs.385496 , Hs.410088, Hs.24835, Hs.446504, Hs.7972, Hs.292057, Hs.435775, Hs.78106, Hs.217754, Hs.153704, Hs.341906, Hs.250830, Hs.68846, Hs .438712, Hs.99480, Hs.434418, Hs.171980, Hs.376810, Hs.324335, Hs.37936, Hs.512546, Hs.128836, Hs.406330, Hs.302352, Hs.169825, Hs.522765 , Hs.526526, Hs.82002, Hs.526394, Hs.512785, Hs.1937 Hs.105700, Hs.122895, Hs.9589, Hs.99439, Hs.334828, Hs.126161, Hs.465202, Hs.310231, Hs.94875, Hs.203557, Hs.444729, Hs.449965, Hs. 122309, Hs.190090, Hs.355714, Hs.153952, Hs.408577, Hs.287370, Hs.185202, Hs.29792, Hs.4845, Hs.27183, Hs.486433, Hs.439463, Hs.194665, Hs.129312, Hs.163682, Hs.73853, Hs.2689, Hs.283900, Hs.162604, Hs.381367, Hs.288218, Hs.203349, Hs.433891, Hs.248065, Hs.440603, Hs. 144913, Hs.199316, Hs.221660, Hs.21554, Hs.510244, Hs.436325, Hs.117979, Hs.356888, Hs.306322, Hs.310431, Hs.356501, Hs.444708, Hs.159609, Hs.18069, Hs.373612, Hs.12802, Hs.508901, Hs.504580, Hs.308242, Hs.128580, Hs.112184, Hs.306298, Hs.343800, Hs.50841, Hs.137516, Hs. 413095, Hs.105640, Hs.144696, Hs.175411, Hs.207407, Hs.406856, Hs.122959, Hs.143792, Hs.170195, Hs.381715, Hs.443292, Hs.56874, Hs.147096, Hs.521267, Hs.196956, Hs.80720, Hs.440953, Hs.352387, Hs.434107, Hs.112720, Hs.155558, Hs.147352, Hs.14632 9, Hs.463171, Hs.445445, Hs.156892, Hs.212670, Hs.129895, Hs.356076, Hs.439590, Hs.278613, Hs.54697, Hs.388917, Hs.55923, Hs.460377, Hs.416244, Hs.234786, Hs.129969, Hs.275711, Hs.127842, Hs.302772, Hs.337008, Hs.48480, Hs.121115, Hs.318773, Hs.98564, Hs.276466, Hs. 78824, Hs.124502, Hs.325530, Hs.375664, Hs.516311, Hs.367688, Hs.386155, Hs.189174, Hs.159264, Hs.386143, Hs.158446, Hs.209461, Hs.163484, Hs.300559, Hs.155223, Hs.448979, Hs.368420, Hs.289044, Hs.74088, Hs.93907, Hs.408264, Hs.439168, Hs.437433, Hs.448468, Hs.310194, Hs. 45152, Hs.283095, Hs.500957, Hs.431156, Hs.511922, Hs.406549, Hs.288731, Hs.123419, Hs.334702, Hs.114172, Hs.408261, Hs.436657, Hs.125300, Hs.203, Hs.287499, Hs.102943, Hs.145949, Hs.248290, Hs.418167, Hs.55481, Hs.434181, Hs.275906, Hs.249126, Hs.147256, Hs.178295, Hs. 385890, Hs.201800, Hs.96633, Hs.445489, Hs.449650, Hs.102708, Hs.433381, Hs.27263, Hs.512782, Hs.347537, Hs .124115, Hs.1270, Hs.349121, Hs.293884, Hs.143943, Hs.12742, Hs.166705, Hs.13209, Hs.77719, Hs.129694, Hs.126906, Hs.151414, Hs.212584 , Hs.232819, Hs.41324, Hs.140821, Hs.351593, Hs.12248, Hs.434281, Hs.283506, Hs.66412.18 Hs.2115872.17 Hs.427712.17 Hs.447703, Hs.372208, Hs.379010, Hs.437008, Hs.99311, Hs.464896, Hs.323079, Hs.509439, Hs.440088, Hs.34045, Hs.406073, Hs.455335, Hs.175465, Hs.507549, Hs.127775, Hs. 132888, Hs.8728, Hs.157231, Hs.62863, Hs.169600, Hs.414332, Hs.259403, Hs.101949, Hs.436339, Hs.128668, Hs.380167, Hs.79340, Hs.43125, Hs.2251, Hs.142296, Hs.288155, Hs.399740, Hs.27524, Hs.165464, Hs.123642, Hs.125156, Hs.58561, Hs.418966, Hs.447121, Hs.24734, Hs. 348709, Hs.89436, Hs.2563, Hs.186363, Hs.169470, Hs.6540, Hs.223206, Hs.439169, Hs.133250, Hs.172562, Hs.349705, Hs.405457, Hs.145894, Hs.7360, Hs.163546, Hs.102276, Hs.511781, Hs.283794, Hs.171090, Hs.508459, Hs.276808, Hs.248155, Hs.43 The Chinese medicine according to claim 30, which is one or more of the genes described in 6042, Hs.371212, Hs.221040 and Hs.374990, a fragment thereof or a complementary sequence chain thereof. An adaptive diagnostic method for Chinese medicine in medicine. The target genes are listed in NCBI Unigene database SEQ ID NOs: Hs. 58323, Hs. 160373, Hs. 334832, Hs. 150136, Hs. 272385, Hs. 382870, Hs. 430919, Hs. 387551, Hs. 194408, Hs. 406961, Hs.164021, Hs.24884, Hs.437211, Hs.436628, Hs.283880, Hs.110299, Hs.390242, Hs.113291, Hs.278894, Hs.90221, Hs.24583, Hs.266902, Hs.148956, Hs.162282, Hs.103388, Hs.406300, Hs.382347, Hs.413430, Hs.449884, Hs.24889, Hs.440606, Hs.36069, Hs.131255, Hs.369557, Hs. 512660, Hs.359981, Hs.283085, Hs.163546, Hs.117938, Hs.74376, Hs.438482, Hs.482392, Hs.320086, Hs.95990, Hs.9469, Hs.108642, Hs.180016, Hs.479572, Hs.169395, Hs.335319, Hs.442801, Hs.111726, Hs.183390, Hs.449407, Hs.434195, Hs.17546, Hs.127951, Hs.458408, Hs.287374, Hs. 150826, Hs.181483, Hs.115467, Hs.232779, Hs.96557, Hs.406722, Hs.201269, Hs.47313, Hs.8852, Hs.283326, Hs.225943, Hs.12902, Hs.500371, Hs.7296, Hs.430854, Hs.278568, Hs.132275, Hs.66392 Hs.437179, Hs.8850, Hs.98967, Hs.57876, Hs.459828, Hs.310230, Hs.25328, Hs.301956, Hs.144875, Hs.110457, Hs.509265, Hs.60339, Hs. 511880, Hs.446152, Hs.48100, Hs.247975, Hs.479080, Hs.490096, Hs.8346, Hs.131555, Hs.32967, Hs.307003, Hs.127032, Hs.109268, Hs.80720, Hs.266738, Hs.113912, Hs.175780, Hs.497972, Hs.18213, Hs.152664, Hs.459847, Hs.378736, Hs.458427, Hs.36761, Hs.429531, Hs.149797, Hs. 444135, Hs.22575, Hs.272191, Hs.512643, Hs.54713, Hs.820, Hs.77221, Hs.253690, Hs.436282, Hs.181289, Hs.170560, Hs.426734, Hs.334527, Hs.406701, Hs.177434, Hs.511968, Hs.389964, Hs.251699, Hs.207805, Hs.140821, Hs.281004, Hs.435675, Hs.292597, Hs.178715, Hs.161394, Hs. 396332, Hs.302109, Hs.86088, Hs.356794, Hs.449392, Hs.134807, Hs.210850, Hs.18705, Hs.326444, Hs.287453, Hs.130522, Hs.234775, Hs.438555, Hs.171532, Hs.54617, Hs.268843, Hs.11713, Hs.5025, Hs.288727, Hs.412022, Hs.78465, Hs.423112, Hs.497707, Hs.2 35390, Hs.33455, Hs.371278, Hs.237642, Hs.234790, Hs.78792, Hs.115181, Hs.115617, Hs.463017, Hs.406789, Hs.378753, Hs.256583, Hs.99430, Hs.525361, Hs.148559, Hs.249181, Hs.23853, Hs.172052, Hs.90821, Hs.444182, Hs.123474, Hs.284310, Hs.137574, Hs.102929, Hs.511985, Hs. 371335, Hs.528103, Hs.355711, Hs.496614, Hs.27621, Hs.183006, Hs.27021, Hs.389571, Hs.227457, Hs.334772, Hs.512760, Hs.53996, Hs.207407, Hs.297997, Hs.512808, Hs.149467, Hs.510932, Hs.272493, Hs.79881, Hs.3052, Hs.415895, Hs.388220, Hs.253193, Hs.124502, Hs.232803, Hs. 269777, Hs.12264, Hs.380763, Hs.147595, Hs.411274, Hs.441481, Hs.376025, Hs.250895, Hs.468922, Hs.161427, Hs.385986, Hs.439760, Hs.313413, Hs.408843, Hs.58974, Hs.430755, Hs.99120, Hs.25363, Hs.287405, Hs.131035, Hs.335355, Hs.259471, Hs.235888, Hs.144527, Hs.414091, Hs. 480174, Hs.445241, Hs.44888, Hs.382683, Hs.439494, Hs.288520, Hs.234027, Hs.130685, Hs.399966, Hs.434486, Hs.407002, Hs.333958, Hs.238648, Hs.308045, Hs.196470, Hs.144531, Hs.7327, Hs.83916, Hs.169441, Hs.73890, Hs.432374, Hs.143751, Hs. 2074, Hs.158278, Hs.158963, Hs.490216, Hs.103422, Hs.133361, Hs.404220, Hs.169071, Hs.288718, Hs.144130, Hs.336224, Hs.112669, Hs.159265, Hs.122110, Hs.7835, Hs.407015, Hs.441708, Hs.452398, Hs.439124, Hs.443020, Hs.499520, Hs.356572, Hs.284200, Hs.78944, Hs.512387, Hs. 235498, Hs.133082, Hs.471423, Hs.73625, Hs.9914, Hs.355862, Hs.82120, Hs.443392, Hs.401954, Hs.436140, Hs.461482, Hs.433596, Hs.434103, Hs.385898, Hs.348920, Hs.81848, Hs.432379, Hs.79025, Hs.407162, Hs.197922, Hs.180171, Hs.408200, Hs.444529, Hs.164649, Hs.4224, Hs. 460471, Hs.149152, Hs.458397, Hs.124211, Hs.415497, Hs.293917, Hs.253489, Hs.14822, Hs.376590, Hs.179112, Hs.152337, Hs.438300, Hs.344099, Hs.421986, Hs.432458, Hs.351414, Hs.54460, Hs.349227, Hs.47312, Hs.185796, Hs.26981, Hs.267038, Hs.448749, H s.170114, Hs.95582, Hs.352601, Hs.353001, Hs.352617, Hs.414164, Hs.30340, Hs.359978, Hs.298241, Hs.178292, Hs.156232, Hs.406681, Hs. 411644, Hs.97933, Hs.137674, Hs.476396, Hs.388877, Hs.155573, Hs.87, Hs.153203, Hs.458434, Hs.10056, Hs.148074 and Hs.377022 The method for adaptive diagnosis of Kampo medicine in Kampo medicine according to Claim 30, wherein the gene is any one or more of the gene, a fragment thereof, or a complementary sequence chain thereof. The target genes are listed in NCBI Unigene database SEQ ID NOs: Hs.436450, Hs.278613, Hs.411239, Hs.445072, Hs.123515, Hs.17518, Hs.128668, Hs.280226, Hs.511758, Hs. 233437, Hs.187802, Hs.61929, Hs.406741, Hs.245431, Hs.20082, Hs.29798, Hs.222, Hs.162089, Hs.37936, Hs.144913, Hs.166351, Hs.313, Hs.522901, Hs.450230, Hs.89679, Hs.323966, Hs.439168, Hs.159629, Hs.186363, Hs.172052, Hs.250723, Hs.356605, Hs.376407, Hs.188021, Hs. 386264, Hs.40510, Hs.408067, Hs.58009, Hs.222909, Hs.113227, Hs.350800, Hs.377070, Hs.178295, Hs.222746, Hs.351811, Hs.440088, Hs.490397, Hs.344400, Hs.361748, Hs.283045, Hs.27258, Hs.25328, Hs.350765, Hs.103665, Hs.194725, Hs.327621, Hs.408629, Hs.411347, Hs.489592, Hs. 386236, Hs.446451, Hs.105115, Hs.45207, Hs.91283, Hs.178499, Hs.444882, Hs.381370, Hs.369994, Hs.93883, Hs.306230, Hs.413924, Hs.504809, Hs.84665, Hs.389724, Hs.211587, Hs.1560, Hs.406733, H s.306812, Hs.348745, Hs.106688, Hs.129895, Hs.388917, Hs.368746, Hs.413525, Hs.166011, Hs.94865, Hs.250472, Hs.434485, Hs.267038, Hs. 445426, Hs.154084, Hs.23317, Hs.75360, Hs.127405, Hs.155584, Hs.47504, Hs.90318, Hs.128499, Hs.283082, Hs.449006, Hs.309365, Hs.436602, Hs.324335, Hs.351816, Hs.306992, Hs.512632, Hs.443260, Hs.348709, Hs.426324, Hs.227656, Hs.435092, Hs.272200, Hs.347537, Hs.271269, Hs. 424414, Hs.707, Hs.248204, Hs.168355, Hs.58559, Hs.407817, Hs.447162, Hs.144870, Hs.435398, Hs.146025, Hs.406485, Hs.326559, Hs.258536, Hs.169825, Hs.1270, Hs.325335, Hs.335877, Hs.421280, Hs.443120, Hs.389638, Hs.124673, Hs.150138, Hs.476618, Hs.438482, Hs.652, Hs. 199372, Hs.493952, Hs.371212, Hs.25156, Hs.301956, Hs.511810, Hs.151408, Hs.157144, Hs.159525, Hs.94327, Hs.449791, Hs.320522, Hs.123114, Hs.446376, Hs.250666, Hs.201554, Hs.351818, Hs.189575, Hs.132270, Hs.36793, Hs.23367, Hs.104613, Hs.254006 Hs.133995, Hs.395205, Hs.476835, Hs.513009, Hs.257584, Hs.5462, Hs.96149, Hs.374720, Hs.46791, Hs.127473, Hs.91813, Hs.271640, Hs. 379010, Hs.516739, Hs.376657, Hs.121490, Hs.352240, Hs.203557, Hs.228982, Hs.122115, Hs.112928, Hs.436975, Hs.387258, Hs.433197, Hs.159553, Hs.437, Hs.288911, Hs.440953, Hs.20315, Hs.104859, Hs.38260, Hs.199316, Hs.511791, Hs.282275, Hs.249126, Hs.163484, Hs.297604, Hs. 73853, Hs.27335, Hs.26176, Hs.92683, Hs.212584, Hs.372616, Hs.153647, Hs.121115, Hs.87306, Hs.318775, Hs.353114, Hs.167679, Hs.415209, Hs.55209, Hs.143600, Hs.156007, Hs.444960, Hs.234016, Hs.283011, Hs.512778, Hs.37107, Hs.414332, Hs.164680, Hs.74115, Hs.422120, Hs. 104133, Hs.21861, Hs.249184, Hs.375054, Hs.436424, Hs.302754, Hs.297452, Hs.165387, Hs.493450, Hs.306094, Hs.414110, Hs.118057, Hs.840, Hs.2207, Hs.343154, Hs.445772, Hs.132272, Hs.59892, Hs.380519, Hs.442936, Hs.274548, Hs.72910, Hs.49433, Hs.266 63, Hs.155814, Hs.212892, Hs.408488, Hs.37125, Hs.375870, Hs.440695, Hs.341906, Hs.370622, Hs.314141, Hs.118631, Hs.115365, Hs.238964, Hs.331491, Hs.194714, Hs.434375, Hs.127012, Hs.306622, Hs.211298, Hs.264330, Hs.347534, Hs.148767, Hs.41270, Hs.283096, Hs.110637, Hs. 76152, Hs.334, Hs.55412, Hs.497743, Hs.276808, Hs.114599, Hs.461085, Hs.282787, Hs.131834, Hs.21838, Hs.492218, Hs.248114, Hs.501321, Hs.512787, Hs.268700, Hs.313948, Hs.211477, Hs.2053, Hs.21388, Hs.507546, Hs.1501, Hs.248139, Hs.283779, Hs.511992, Hs.406984, Hs. 145680, Hs.403836, Hs.287656, Hs.53263, Hs.257541, Hs.413265, Hs.333742, Hs.156346, Hs.441287, Hs.131101, Hs.436983, Hs.256036, Hs.420036, Hs.440387, Hs.122309, Hs.479817, Hs.195614, Hs.355584, Hs.65248, Hs.512782, Hs.363315, Hs.43107, Hs.233950, Hs.445445, Hs.82316, Hs. Inheritance of any one or more of the genes described in 148990, Hs.158798, Hs.189067 and Hs.90797 Adaptive method of diagnosing Chinese medicine in Chinese medicine according to claim 30 which is a fragment or complementary sequence strand thereof.   The Chinese herbal medicine is Hanka-koboku-to, and the target gene may change in expression due to the appearance / disappearance of “Hakkatsu-koboku-no-proof”, the use of Hanka-koboku-to, and / or the effectiveness of Hanka-koboku-to. 30. The method for adaptive diagnosis of Chinese medicine in Chinese medicine according to claim 29. The target gene is any one or more genes, fragments thereof, or their complementary sequence chains of the base sequence described in SEQ ID NO: 290 to SEQ ID NO: 338 of the sequence listing or a part thereof. 37. The method for adaptive diagnosis of Chinese medicine in Chinese medicine according to claim 36.