JP2005319395A - Decomposition method of high bod specimen, urea, ammonia, and nitrate - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To realize decomposition of nitrogen-containing urea, ammonia, and nitrate in the environment, such as agricultural effluent, domestic effluent, and industrial wastewater, and decomposition of a specimen having high BOD at low energy and cost with a small-scale apparatus. <P>SOLUTION: Bacillus midousuji obtained by fresh culture etc. is added to the high BOD specimen, urea, ammonia, and nitrate, and the specimen is left still at 62 to 65°C for several hours to several days. As a result, the high BOD specimen, urea, ammonia, and nitrate are decomposed by Bacillus midousuji. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a method for decomposing high BOD samples, urea, ammonia, and nitrates.

  Nitrogen is an essential nutrient for living organisms. In agriculture, nitrogen compounds are regularly applied as fertilizers. In addition to fertilizers, large amounts of manure containing nitrogen are generated when raising livestock such as cattle. Wastewater containing a large amount of nitrogen is generated not only in agricultural wastewater but also in domestic wastewater, and when such wastewater flows into rivers and lakes, the eutrophication of rivers and the like progresses, causing red tides and water pollution. There is a report that it is. The emission regulations on nitrogen in the country are becoming stricter, and how to remove nitrogen from agricultural wastewater, domestic wastewater, industrial wastewater, etc. is becoming a problem. In addition, high BOD samples that contain a large amount of protein as well as nitrogen and have a high BOD value are also being investigated as a cause of eutrophication of rivers and lakes.

Conventionally, there are technologies such as Non-Patent Document 1 and Non-Patent Document 2. However, when removing nitrogen from domestic wastewater or agricultural wastewater, there are cases where four large water tanks may be used, and the facilities are large. In addition, the processing cost may be tens of millions of yen, which is a problem from the viewpoint of cost performance.
Water Pollution Control Technology Overview, Chemical Industry Association, Bafukan, 157p to 169p Technology and Anaerobic Treatment Field (2) Nitrogen removal technology using Anammox reaction, Hidei Yasui, Takaaki Tokutomi, Environmental Technology, vol33, No2, 2004, 46p to 49p Toda New Bacteriology, Nanzando, Shinichi Yoshida, Yusuke Yanagi, Rev. 32, ISBN4-525-16012-8, 67p, 844p

  The problem to be solved is how to efficiently decompose a high BOD sample, urea, ammonia, and nitrate with a small apparatus.

  The present invention relates to a biologically pure culture of a Bacillus midousuji cell (a microorganism deposited as ATCC accession numbers 55926 and 202050 or a mutant strain derived therefrom in a high BOD sample, urea, ammonia, and nitrate. , Hereinafter referred to as "Bacillus midousuji cells"), and the most important feature is to decompose by mixing.

  According to the present invention, there is an advantage that a high-BOD sample, urea, ammonia, and nitrate can be decomposed without a processing cost with a small apparatus.

  Disassembly was realized by performing minimum management such as temperature control and intake / exhaust control using a small device.

First, a method for decomposing a high BOD sample containing a large amount of protein as well as nitrogen and having a high BOD value will be described. A blood collection has been (30 minutes at 140 ° using a Hitachi Rotokurebu ^TM) Serum Plasma residue per high-pressure steam sterilization of blood was pooled human serum plasma dilutions 200L for use in biochemical tests of human, Bacillus midousuji cells A sample prepared by adding 4 g (wet amount of bacteria) of freshly cultured strains from Trypticase Soy Agar (BBL) agar medium at 65 ° C. for 3 hours to a high protein digester (Kodachi Machinery Manufacturing Co., Ltd., processing tank 200 liters, storage) Using a tank of 300 liters), a sample was placed in a processing tank, mixed with air, and stirred at 65 ° C. while evacuating. Table 1 shows the results of measuring the BOD of treated water after 24 hours and treated water after 48 hours with a simple BOD measuring device (manufactured by Horiba Seisakusho).

  According to this experimental result, the BOD value decreased by about 96%, indicating that the protein and the like were decomposed by Bacillus midousuji cells. Further, when the experiment was conducted without exhausting, it was necessary to exhaust because the BOD value increased conversely.

  Next, a method for decomposing urea into ammonia will be described. A sample prepared by mixing Bacillus midousuji cells in Soybean-Casein Digest Broth, put it in the trade name Staturease “Asuka” (sold by Asuka Pure Chemicals), set the temperature at 65 ° C. and let stand for 2 hours did. As a result, the sample showed a bright red color, indicating that urea was decomposed into ammonia.

Next, a method for decomposing ammonia into nitrite will be described. NaNO ₃ 0.25 g, 2.94 mM, MgSO ₄ 0.2 g, 1.66 mM, Na ₂ HPO ₄ 0.21 g, 1.48 mM, KCl 0.5 g, 6.70 mM, NH ₄ Cl 0.25 g, 4.67 mM, NaH ₂ PO ₄ 0.09 g, 7.50 mm, the CaCl ₂ 0.02g, 1.80mM, FeSO ₄ and 1.0 mg, to create a mineral salts medium of 6.58μM containing 1L, the inorganic salt medium in a glass test tube with lined screw cap 7ml A 2 mL sample of inorganic salt medium dispensed in 2 mL was prepared.

Bacteria are strains of Bacillus midousuji cells that were freshly cultured in Trypticase Soy Agar (BBL) flat salt salt at 65 ° C for 3 hours and adjusted to a concentration of 10 ⁹ / mL with sterile purified water. A sample added with mL was prepared. Moreover, the sample which does not add bacteria as a control system was prepared. After both samples were reacted at 62 degrees for 11 hours, the ammonia nitrogen concentration (including the definition of ammoniacal nitrogen) was measured. The measurement method was based on Amicheck ^™ (Arkray, Inc.). Both samples were diluted for easy measurement by Amicheck ^™ . The experimental results are shown in Table 2. However, theoretically, it is 0.09 g / L without Bacillus midousuji cells, but it seems that there are errors due to the measurement of Amicheck ^™ . However, ammonia nitrogen is decreasing.

  The sample to which Bacillus midousuji cells were added decreased to an ammonia nitrogen concentration of 0.046 g / L, whereas the ammonia nitrogen concentration of the sample to which Bacillus midousuji cells were not added was 0.159 g / L. It is thought that ammonia was decomposed into nitrate by the oxidation reaction of Bacillus midousuji cells.

Mention another experiment. A blood collection has been (30 minutes at 140 ° using a Hitachi Rotokurebu ^TM) Serum Plasma residue per high-pressure steam sterilization of blood was pooled human serum plasma dilutions 200L for use in biochemical tests of human, Bacillus midousuji cells A sample prepared by adding 4 g (wet bacteria amount) of freshly cultured strains from Trypticase Soy Agar (BBL) agar medium at 65 ° C. for 3 hours to a high protein digester (Kodama Machinery Co., Ltd., processing tank 200 liters, storage) A sample was placed in the treatment tank using a tank of 300 liters), and the sample was reacted at 62 ° C. for 3 to 21 hours to measure the ammonia nitrogen concentration. The method of measurement was by Ami check ^TM in the same way. The experimental results are shown in Table 3.

  When Bacillus midousuji cells were added, the ammonia nitrogen concentration decreased about 30% in 3 hours and about 40% in 21 hours. It is thought that ammonia was decomposed into nitrate by the oxidation reaction of Bacillus midousuji cells. Further, Bacillus midousuji cells have a more metabolic reaction even at 65 degrees.

I will mention another experiment. A blood collection has been (30 minutes at 140 ° using a Hitachi Rotokurebu ^TM) Serum Plasma residue per high-pressure steam sterilization of blood was pooled human serum plasma dilutions 200L for use in biochemical tests of human, Bacillus midousuji cells A sample prepared by adding 4 g (wet bacteria amount) of freshly cultured strains from Trypticase Soy Agar (BBL) agar medium at 65 ° C. for 3 hours to a high protein digester (Kodama Machinery Co., Ltd., processing tank 200 liters, storage) The sample tank was mixed with air in a treatment tank using a tank of 300 liters) and stirred at 65 ° C. Results of measuring the hydrogen ion concentration of raw water, treated water after 24 hours, treated water after 48 hours with a glass electrode type hydrogen ion concentration meter (manufactured by Horiba) and the same method in another experiment Table 4 shows the results of measuring the hydrogen ion concentration of the treated water, the treated water after 48 hours, and the treated water after 72 hours.

  According to this experimental result, the hydrogen ion concentration decreased rapidly, indicating that the alkaline ammoniacal substance was decomposed by Bacillus midousuji cells. It is thought that ammonia was decomposed into nitrate by the oxidation reaction of Bacillus midousuji cells.

Next, a method for decomposing nitrate will be described. Bacteria obtained by cultivating a strain of Bacillus midousuji cells on Trypticase Soy Agar (BBL) agar medium at 65 ° C. for 3 hours in a nitrate medium was added at 10 ⁸ / mL, and the mixture was sealed and cultured for 11 hours, and a nitrate reduction test was performed. When the nitrite reagent was added after incubation, the sample did not turn red. For this reason, it was found that no nitrite was produced in the sample. Further, a small amount of zinc powder was added to the sample and left for a while. However, it was found that the sample was free of nitrate because there was no change. The nitrate of the sample is considered to have been decomposed into nitrogen gas etc. by the reduction reaction of Bacillus midousuji cells.

  FIG. 1 is an explanatory view showing a decomposition treatment cycle using Bacillus midousuji cells. If Bacillus midousuji cells are used, urea is oxidized and decomposed into ammonia, and ammonia is oxidized and decomposed into nitrite. If nitrite is further oxidized to nitrate and Bacillus midousuji cells are used, nitrate can be reduced and decomposed into nitrogen gas and the like.

  Bacillus midousuji cells oxidize compounds such as decomposition of high BOD materials under aerobic conditions. On the other hand, under anaerobic conditions, it has the function of reducing compounds such as decomposing nitrate into nitrogen gas. Bacillus midousuji cells are rare in that they have a function of causing an oxidation reaction or a reduction reaction in one cell.

  INDUSTRIAL APPLICABILITY The present invention can be applied to the treatment of wastewater containing nitrogen and wastewater containing high protein such as domestic wastewater, agricultural wastewater, industrial wastewater and other medical waste.

It is explanatory drawing which showed the decomposition process cycle using Bacillus midousuji microbial cell. (Example 5)

Claims (6)

  Bacillus midousuji cells (biologically pure cultures of microorganisms deposited as ATCC deposit numbers 55926 and 202050 or mutant strains derived from them) and high BOD samples are mixed and mixed under high aerobic conditions. A method for decomposing BOD samples.   A method of decomposing urea by mixing Bacillus midousuji cells (biologically pure culture of microorganisms deposited as ATCC deposit numbers 55926 and 202050 or mutant strains derived therefrom) with urea.   A method of decomposing ammonia by mixing Bacillus midousuji cells (biologically pure culture of microorganisms deposited as ATCC deposit numbers 55926 and 20950 or mutant strains derived therefrom) and ammonia.   Bacillus midousuji cells (biologically pure culture of microorganisms deposited as ATCC deposit numbers 55926 and 202050 or mutant strains derived from them) and nitrate are mixed to decompose nitrate into nitrogen gas Method.   Utilizing Bacillus midousuji cells (biologically pure culture of microorganisms deposited as ATCC deposit numbers 55926 and 202050 or mutant strains derived from them), urea is decomposed into ammonia and ammonia Is a method that decomposes nitrate into nitrite, which is then decomposed into nitrogen gas and nitrous oxide.   Oxidizing compounds using a kind of fungus, Bacillus midousuji (a biologically pure culture of microorganisms deposited as ATCC Deposit Nos. 55926 and 20950 or mutant strains derived from it) A method for reducing a compound.

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