JP2005283567A5 - - Google Patents

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Publication number
JP2005283567A5
JP2005283567A5 JP2005037492A JP2005037492A JP2005283567A5 JP 2005283567 A5 JP2005283567 A5 JP 2005283567A5 JP 2005037492 A JP2005037492 A JP 2005037492A JP 2005037492 A JP2005037492 A JP 2005037492A JP 2005283567 A5 JP2005283567 A5 JP 2005283567A5
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Prior art keywords
cellulose
protein
bacterial cellulose
producing
substrate
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Pending
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JP2005037492A
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Japanese (ja)
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JP2005283567A (en
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Priority to JP2005037492A priority Critical patent/JP2005283567A/en
Priority claimed from JP2005037492A external-priority patent/JP2005283567A/en
Publication of JP2005283567A publication Critical patent/JP2005283567A/en
Publication of JP2005283567A5 publication Critical patent/JP2005283567A5/ja
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基板上に水を含浸したバクテリアセルロース層を形成する工程と、
前記バクテリアセルロース層上に脂質二重層を形成する工程と、
前記脂質二重層にタンパク質を埋め込む工程と、
を含むプロテインチップの製造方法。 Forming a bacterial cellulose layer impregnated with water on a substrate;
Forming a lipid bilayer on the bacterial cellulose layer;
Embedding protein in the lipid bilayer;
A method for producing a protein chip comprising 基板上にバクテリアセルロース層を形成する工程と、
前記バクテリアセルロース層を整然と並んだアレイ要素からなるバクテリアセルロースアレイに加工する工程と、
前記アレイ要素上に脂質二重層を形成する工程と、
前記脂質二重層にタンパク質を埋め込む工程と、
を含むプロテインチップの製造方法。 Forming a bacterial cellulose layer on the substrate;
Processing the bacterial cellulose layer into a bacterial cellulose array consisting of ordered array elements;
Forming a lipid bilayer on the array element;
Embedding protein in the lipid bilayer;
A method for producing a protein chip comprising バクテリアセルロースのシートを整然と並んだアレイ要素からなるバクテリアセルロースアレイに加工する工程と、
前記バクテリアセルロースアレイを基板上に固定する工程と、
前記アレイ要素上に脂質二重層を形成する工程と、
前記脂質二重層にタンパク質を埋め込む工程と、
を含むプロテインチップの製造方法。 Processing bacterial cellulose sheets into a bacterial cellulose array consisting of ordered array elements;
Fixing the bacterial cellulose array on a substrate;
Forming a lipid bilayer on the array element;
Embedding protein in the lipid bilayer;
A method for producing a protein chip comprising 互いに隣接する前記アレイ要素間の基板表面を疎水処理する工程を更に含む請求項２または３に記載の製造方法。   The manufacturing method according to claim 2, further comprising a step of subjecting a substrate surface between the array elements adjacent to each other to a hydrophobic treatment. 前記バクテリアセルロースはナタデココ又はホヤセルロースである、ことを特徴とする請求項１〜４のいずれかの製造方法。   The method according to any one of claims 1 to 4, wherein the bacterial cellulose is Nata de Coco or squirt cellulose. 水を含浸したバクテリアセルロースからなるアレイ要素を基板上に整然と並べて固定するとともに、タンパク質を埋め込んだ脂質二重層を各アレイ要素上に形成してなるプロテインチップ。   A protein chip in which array elements made of bacterial cellulose impregnated with water are arranged and fixed on a substrate in an orderly manner, and a lipid bilayer in which proteins are embedded is formed on each array element. 互いに隣接するアレイ要素間の基板表面を疎水処理してなる請求項６のプロテインチップ。   The protein chip according to claim 6, wherein the substrate surface between adjacent array elements is subjected to a hydrophobic treatment. 前記バクテリアセルロースはナタデココ又はホヤセルロースである、ことを特徴とする請求項６又は７のプロテインチップ。   The protein chip according to claim 6 or 7, wherein the bacterial cellulose is Nata de Coco or squirt cellulose. 請求項６〜８のいずれかのプロテインチップを作成するためのプレートであって、
水（タンパク質を含む水溶液を除く。）を含浸したバクテリアセルロースまたは水を含浸する前のバクテリアセルロースを基板上に固定してなるプロテインチップ作成用プレート。 A plate for producing the protein chip according to claim 6,
A plate for producing a protein chip in which bacterial cellulose impregnated with water (excluding an aqueous solution containing protein) or bacterial cellulose before impregnated with water is fixed on a substrate. タンパク質を埋め込んだ脂質二重層を、含水状態のバクテリアセルロース上に形成したことを特徴とするタンパク質保持方法。   A protein retention method characterized in that a lipid bilayer in which protein is embedded is formed on water-containing bacterial cellulose. 前記バクテリアセルロースはナタデココ又はホヤセルロースである、ことを特徴とする請求項１０のタンパク質保持方法。   The protein retention method according to claim 10, wherein the bacterial cellulose is Nata de Coco or squirt cellulose. バクテリアセルロースまたはホヤセルロースをタンパク質を含む水溶液中に浸漬することにより、タンパク質を含む水溶液を含浸したセルロースを作成する工程と、
当該セルロースからなるセルロース層を基板上に固定する工程と、
を含むプロテインチップの製造方法。 A step of making cellulose impregnated with an aqueous solution containing protein by immersing bacterial cellulose or squirt cellulose in an aqueous solution containing protein;
Fixing the cellulose layer comprising the cellulose on the substrate;
A method for producing a protein chip comprising 前記セルロース層を整然と並んだプローブセルからなるプローブセルアレイに加工する工程を更に含む請求項１２に記載のプロテインチップの製造方法。   The method for producing a protein chip according to claim 12, further comprising a step of processing the cellulose layer into a probe cell array comprising orderly arranged probe cells. バクテリアセルロースまたはホヤセルロースをタンパク質を含む水溶液中に浸漬することにより、タンパク質を含む水溶液を含浸したセルロースを作成する工程と、
当該セルロースからなるプローブセルを基板上に整然と並べて固定する工程と、
を含むプロテインチップの製造方法。 A step of making cellulose impregnated with an aqueous solution containing protein by immersing bacterial cellulose or squirt cellulose in an aqueous solution containing protein;
A step of arranging and fixing the probe cells made of cellulose in an orderly manner on a substrate;
A method for producing a protein chip comprising 互いに隣接するプローブセル間の基板表面を疎水処理する工程を更に含む請求項１３または１４に記載のプロテインチップの製造方法。   The method for producing a protein chip according to claim 13 or 14, further comprising a step of subjecting the substrate surface between adjacent probe cells to a hydrophobic treatment.
JP2005037492A 2005-02-15 2005-02-15 Protein chip Pending JP2005283567A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2005037492A JP2005283567A (en) 2005-02-15 2005-02-15 Protein chip

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Application Number Priority Date Filing Date Title
JP2005037492A JP2005283567A (en) 2005-02-15 2005-02-15 Protein chip

Publications (2)

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JP2005283567A JP2005283567A (en) 2005-10-13
JP2005283567A5 true JP2005283567A5 (en) 2008-03-27

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0403139D0 (en) * 2004-12-23 2004-12-23 Nanoxis Ab Device and use thereof
JP5055971B2 (en) * 2006-11-16 2012-10-24 株式会社ニコン Surface treatment method, surface treatment apparatus, exposure method, exposure apparatus, and device manufacturing method
EP2011630A1 (en) * 2007-07-03 2009-01-07 F. Hoffmann-La Roche AG Method for manufacturing an analytical element
JP5230300B2 (en) * 2008-08-26 2013-07-10 日本電信電話株式会社 Biomolecule function analysis substrate, biomolecule function analysis sample body, and biomolecule function analysis method
JP6004302B2 (en) * 2010-02-01 2016-10-05 学校法人立教学院 Processed Nata de Coco formation method, Processed Nata de Coco restoration method, and Processed Nata de Coco
JP6004331B2 (en) * 2011-09-08 2016-10-05 学校法人立教学院 Processed cellulose airgel and method for producing the same, and method for producing regenerated cellulose hydrogel
JP5770615B2 (en) * 2011-12-08 2015-08-26 日本電信電話株式会社 Method for producing lipid bilayer membrane substrate

Family Cites Families (5)

* Cited by examiner, † Cited by third party
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JP2003511679A (en) * 1999-10-08 2003-03-25 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ Lipid bilayer array and methods of making and using same
JP4378042B2 (en) * 2000-08-31 2009-12-02 キヤノン株式会社 Method for detecting target component in specimen sample, and detection substrate used therefor
JP2003028864A (en) * 2001-07-13 2003-01-29 Fuji Photo Film Co Ltd Substrate for dna chip, its manufacturing method, and dna chip manufacturing method
JP2005538377A (en) * 2002-09-11 2005-12-15 シナメム コーポレイション Membrane-based assay
JP5334006B2 (en) * 2004-07-05 2013-11-06 株式会社発明屋 Protein chip

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