JP2005283567A5 - - Google Patents

Claims (15)

Forming a bacterial cellulose layer impregnated with water on a substrate;
Forming a lipid bilayer on the bacterial cellulose layer;
Embedding protein in the lipid bilayer;
A method for producing a protein chip comprising Forming a bacterial cellulose layer on the substrate;
Processing the bacterial cellulose layer into a bacterial cellulose array consisting of ordered array elements;
Forming a lipid bilayer on the array element;
Embedding protein in the lipid bilayer;
A method for producing a protein chip comprising Processing bacterial cellulose sheets into a bacterial cellulose array consisting of ordered array elements;
Fixing the bacterial cellulose array on a substrate;
Forming a lipid bilayer on the array element;
Embedding protein in the lipid bilayer;
A method for producing a protein chip comprising   The manufacturing method according to claim 2, further comprising a step of subjecting a substrate surface between the array elements adjacent to each other to a hydrophobic treatment.   The method according to any one of claims 1 to 4, wherein the bacterial cellulose is Nata de Coco or squirt cellulose.   A protein chip in which array elements made of bacterial cellulose impregnated with water are arranged and fixed on a substrate in an orderly manner, and a lipid bilayer in which proteins are embedded is formed on each array element.   The protein chip according to claim 6, wherein the substrate surface between adjacent array elements is subjected to a hydrophobic treatment.   The protein chip according to claim 6 or 7, wherein the bacterial cellulose is Nata de Coco or squirt cellulose. A plate for producing the protein chip according to claim 6,
A plate for producing a protein chip in which bacterial cellulose impregnated with water (excluding an aqueous solution containing protein) or bacterial cellulose before impregnated with water is fixed on a substrate.   A protein retention method characterized in that a lipid bilayer in which protein is embedded is formed on water-containing bacterial cellulose.   The protein retention method according to claim 10, wherein the bacterial cellulose is Nata de Coco or squirt cellulose. A step of making cellulose impregnated with an aqueous solution containing protein by immersing bacterial cellulose or squirt cellulose in an aqueous solution containing protein;
Fixing the cellulose layer comprising the cellulose on the substrate;
A method for producing a protein chip comprising   The method for producing a protein chip according to claim 12, further comprising a step of processing the cellulose layer into a probe cell array comprising orderly arranged probe cells. A step of making cellulose impregnated with an aqueous solution containing protein by immersing bacterial cellulose or squirt cellulose in an aqueous solution containing protein;
A step of arranging and fixing the probe cells made of cellulose in an orderly manner on a substrate;
A method for producing a protein chip comprising   The method for producing a protein chip according to claim 13 or 14, further comprising a step of subjecting the substrate surface between adjacent probe cells to a hydrophobic treatment.