JP2005279619A - Rotary reaction apparatus and rotatively reacting method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a rotary reaction apparatus which can be made compact and in which a flowing mode of a group of fine particles in a liquid can be set freely by gathering the group of fine particles on a fixed whirling orbit and to provide a rotatively reacting method. <P>SOLUTION: A reactive fluid 4 and a magnetic fine particle 5 are injected into a gap of a coaxial double-cylinder vessel 3 having the height being several times of the gap to be formed between two cylinders different in diameter when they are arranged concentrically. The side faces of both or one of two cylinders of the double-cylinder vessel 3 are formed into permeation membrane walls 6 each having a porous membrane through which the magnetic fine particle 5 or a reactive substance can be permeated. The temperature of the reactive fluid is managed by a thermostatic unit 7. Both or one of two cylinders of the coaxial double-cylinder vessel 3 are rotated by a rotation mechanism 8 and a rotation controlling unit 9 for controlling the rotational speed of the rotation mechanism 8. An electromagnet 10 is arranged outside the coaxial double-cylinder vessel 3 for forming a magnetic field in the gap between two cylinders and the magnetic field distribution and magnetic field intensity of the electromagnet are controlled by a magnetic field controlling unit 11. A circulation unit 12 is arranged for making the reactive fluid 4 permeated through one of the permeation membrane walls 6 flow in the other cylinder through the wall. The indicated values of the magnetic field controlling unit, the rotation controlling unit and the thermostatic unit are displayed on a display unit 13. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a rotary reaction apparatus and a rotary reaction method using a Taylor vortex flow of a coaxial double cylinder having a small aspect ratio.

  Conventionally, reaction apparatuses using Taylor vortex flow include apparatuses such as microbial culture, bioreactor, stirring / mixing, fine particle production, and suspension polymerization.

  Here, the Taylor vortex flow is a donut-like shape that appears in the fluid when the outer cylinder is fixed and the inner cylinder is rotated at a rotation speed exceeding a certain critical value by filling the gap between the coaxially arranged double cylinders. It is a vortex. A method of obtaining uniform droplets using a gentle vortex flow using a coaxial double cylinder having a long container length, that is, a large aspect ratio with respect to the double cylinder gap has been reported. (For example, refer to Patent Document 1).

  In addition, there is a bioreactor that utilizes the Taylor vortex flow having such a large aspect ratio and mixes a biocatalyst with a proteolytic solution to improve the decomposition effect. (For example, refer to Patent Document 2 and Patent Document 3.)

  In addition, a method is disclosed in which cell culture is performed in a Taylor vortex flow using a microcarrier to which animal cells are attached (see, for example, Patent Document 4).

  In contrast to the above-mentioned Taylor vortex flow with a large aspect ratio, the container length is short with respect to the gap between the double cylinders, that is, the vortex flow of a coaxial double cylinder with a small aspect ratio increases the rotation speed slowly while stabilizing the flow. There is a flow state called the main mode that is obtained when it is applied, and a flow state called the secondary mode that is obtained by rapidly accelerating the rotation speed. In addition, this secondary mode has an even number of vortices and both ends. There is a normal mode in which the flow of the vortex is the same as the main mode, and a variant mode in which the flow from the inner cylinder toward the outer cylinder is at one or both ends.

  In such a Taylor vortex flow having a small aspect ratio, there are several flow field modes depending on the rotation condition of the cylinder as described above. For example, when the aspect ratio is 3, that is, when a container having a height three times that of the coaxial double cylindrical gap is used, the normal two-cell mode as the above-mentioned main mode in which two vortices appear and the secondary mode There are a total of four types of 4 vortex modes: 4 normal 4 cell modes, 3 vortex variations 3 cell modes, 4 vortex flow variations and 4 variability flow modes opposite to the normal 4 cell mode vortex flow direction. There is a flow field mode.

  In addition, the flow field mode of Taylor vortex flow with a small aspect as described above may have different appearance rates of the flow field mode with changes in the initial rotational angular velocity and rotation speed of the inner cylinder, and mode change occurs. There is a case. For example, Non-Patent Document 1 and Non-Patent Document 2 disclose changes in the flow field mode in a low aspect ratio Taylor vortex flow.

  Further, a chaotic mixing method using magnetic particles is disclosed as a micro-flow mixing method. (For example, refer nonpatent literature 3.).

JP-A-56-139122 (page 1-2, FIG. 1) JP-A-7-123972 (page 2-6, FIG. 3) JP-A-7-298871 (page 2-7, FIG. 5) JP-A-8-308560 (Page 2-5, Fig. 1) Naoto Omura and three others, “Vortex generation mechanism and turbulent transition characteristics of Taylor Couette flow with small aspect ratio”, 1997, Chemical Engineering Vol. 23, No. 6, p741-748 Hiroyuki Furukawa and two others, “Taylor vortex flow with small aspect ratio (change of flow shape between mutation and normal mode and transition process of unsteady mode)”, 2002, Transactions of the Japan Society of Mechanical Engineers (B-bias) 69 Volume 674, p2671-2678 Hiroaki Suzuki and two others, “A Chaotic Micromixer Using Magnetic Particles”, 2003, Japan Society of Mechanical Engineers, Vol. 69, No. 688, p 2626-2632

  However, in the conventional reaction method using a coaxial double cylinder container with a large aspect ratio using Taylor vortex, the mode of vortex flow does not change greatly, so there is a problem that an efficient reaction by flow field mode cannot be selected. .

  In addition, in the coaxial double cylindrical container having a large aspect ratio, the reaction layer is promoted, and there is an obstacle to the installation place and compactness.

  In addition, when the above-described microorganism cultivation method is applied to a coaxial double cylindrical container having a small aspect ratio for compactness, the flow field mode changes due to the change in the microorganism concentration during the cultivation process, and the flow field mode with good culture efficiency. There is a problem that it becomes difficult to change to.

The present invention has been made in view of such problems,
It is an object of the present invention to provide a rotary reaction device and a rotary reaction method in which the apparatus can be made small, a group of fine particles in a liquid can be collected in a fixed orbit, and a flow field mode can be set freely.

  The rotary reaction apparatus of the present invention comprises a coaxial double cylindrical container having a height several times the clearance between both cylinders when two cylinders having different diameters are arranged concentrically, and a gap between the coaxial double cylindrical containers. The reaction fluid to be filled, the magnetic fine particles mixed in the reaction fluid, the thermostat that controls the temperature of the reaction fluid, the rotation mechanism that rotates both or any one of the cylinders in the coaxial double cylinder container, and the rotation of the rotation mechanism , A permeation membrane wall having a porous membrane through which magnetic fine particles or reactants can permeate the side surfaces of both or one of the cylinders in a coaxial double cylindrical container, and a reaction liquid that permeates the permeation membrane wall A circulation device that flows in from the other cylindrical wall, an electromagnet that forms a magnetic field in a cylindrical gap disposed outside the coaxial double cylindrical vessel, and a magnetic field that controls the magnetic field distribution and strength of the electromagnet And control device, and has a display device that shows an indication of the magnetic field control device and a rotation control device.

  Further, the rotational reaction apparatus and the rotational reaction method of the present invention are a process of installing a coaxial double cylindrical container having a height several times the clearance between both cylinders when two cylinders having different diameters are arranged concentrically. And a step of filling the reaction fluid in the gap between the coaxial double cylindrical containers, a step of mixing magnetic fine particles in the reaction fluid, a step of managing the temperature of the reaction fluid, and / or one of the coaxial double cylindrical containers. The step of rotating the cylinder, the step of controlling the rotational angular velocity and the number of rotations of the cylinder, the step of allowing the magnetic fine particles or the reactant to permeate the side of one of the cylinders in the coaxial double cylindrical container, The step of circulating the solution and the substance from the other cylindrical wall, the step of forming a magnetic field in a cylindrical gap arranged outside the coaxial double cylindrical vessel, the magnetic field distribution and the magnetic field strength And controlling the magnetic field distribution, the magnetic field strength, the rotational angular velocity, rotational speed, and has a step of indicating the value of a parameter such as temperature.

The present invention has the following effects.
According to the rotary reaction device and the rotary reaction method of the first aspect of the present invention, since the flow field of the coaxial double cylindrical container having a small aspect ratio is provided, the device can be made small, and the group of fine particles in the liquid can be in a constant orbit. Collectively, the flow field mode optimal for the reaction can be easily formed.

  In addition, according to the rotary reaction device and the rotary reaction method of the second aspect of the present invention, since the coaxial double cylindrical flow having a small aspect ratio and the magnetic field control process of the flow field are included, the application and release of the magnetic field are controlled. Thus, a necessary flow field mode can be selected.

The best mode for carrying out the present invention will be described below with reference to the drawings.
First, the best mode for carrying out the first invention according to the rotary reaction device and the rotary reaction method will be described. FIG. 1 illustrates the apparatus of the present invention.

  The present invention uses a coaxial double-cylinder Taylor vortex flow with a small aspect ratio to collect a group of fine particles in a liquid in a fixed orbit and form a complex and gentle flow field, thereby increasing the reaction efficiency. The present invention provides a rotational reaction device and a rotational reaction method capable of forming a necessary flow field mode by mixing magnetic fine particles in a reaction liquid and applying an external magnetic field. As shown in FIG. 1, the measurement unit 1 and the control unit 2 are included.

  In the gap between the coaxial double cylindrical containers 3, magnetic fine particles 5 are mixed and injected into the reaction fluid 4.

  The reaction fluid 4 is kept at a predetermined temperature by the thermostatic device 7.

  The inner cylinder of the coaxial double cylindrical container 3 is a hollow circular tube, and its wall is made of a permeable membrane wall 6.

  The rotation mechanism 8 is connected to both or one of the coaxial double cylindrical containers 3 and can rotate the cylinder. The rotation angular velocity and the rotation speed of the rotation mechanism 8 are controlled by the rotation control device 9, and can be set so that a Taylor vortex flow is formed at a rotation speed higher than a certain critical value.

  An electromagnet 10 is installed outside the coaxial double cylindrical container 3, and the magnetic field distribution and the magnetic field strength are controlled using the magnetic field control device 11.

  The circulation device 12 adjusts the flow rate of the reaction solution and controls the mixing and removal of magnetic fine particles as necessary in order to control the reaction solution concentration and reaction rate in the coaxial double cylindrical gap.

  Indication values and measurement values of the rotation control device 9, the magnetic field control device 11, and the constant temperature device 7 are displayed on the display device 13.

From the above, according to the best mode for carrying out the present invention,
Since it has a flow field of a coaxial double cylindrical container with a small aspect ratio, the apparatus can be made small, and a flow field mode optimal for the reaction can be easily formed by collecting a group of fine particles in a liquid in a fixed orbit. .

  The present invention is not limited to the best mode for carrying out the above-described invention, and various other configurations can be adopted without departing from the gist of the present invention.

  Next, the best mode for carrying out the second aspect of the rotary reaction device and the rotary reaction method will be described. FIG. 2 illustrates the method of the present invention.

As shown in FIG. 1, magnetic fine particles and a reaction liquid are injected into the interval between coaxial double cylinders.
As shown in FIG. 2, the coaxial double cylinder is made stationary under temperature control to stabilize the liquid.
As shown in FIG. 3, a predetermined magnetic field distribution and magnetic field strength are set by a magnetic field control device, and a magnetic field is applied by an electromagnet.
As shown in Fig. 4, the rotation control device is used to set the rotation angular velocity and the rotation speed of the rotation mechanism, and both or one of the coaxial double cylinders is rotated by the rotation mechanism.
As shown in FIG. 5, the magnetic field of the electromagnet is released by the magnetic field control device as soon as the cylinder reaches a predetermined rotational speed.
As shown in FIG. 6, it is determined whether the flow field has been shifted to a predetermined flow field mode. If not displaced to the predetermined flow field mode, return to 2.
As shown in FIG. 7, a constant rotation operation is performed by the rotation control device, and the reaction is started.
As shown in FIG. 8, it is determined whether or not magnetic fine particles are necessary for the reaction.
As shown in FIG. 9, when no magnetic fine particles are required for the reaction, the magnetic particles are recovered using a circulation device.
As shown in FIG. 10, it is judged whether the reaction reaches a critical value. If the critical value is not reached, the reaction is continued at a constant rotation.
As shown in FIG. 11, the reaction solution is recovered using a circulation device.
The process ends at 12.

From the above, according to the best mode for carrying out the invention,
Since it has a coaxial double cylindrical flow with a small aspect ratio and a magnetic field control step for the flow field, the required flow field mode can be selected by controlling the application and release of the magnetic field.

  The present invention is not limited to the best mode for carrying out the above-described invention, and various other configurations can be adopted without departing from the gist of the present invention.

  Next, the first embodiment according to the present invention will be described in detail. However, it goes without saying that the present invention is not limited to these examples.

  Here, an example of a method for collecting fine particles in a flow field in a fixed orbit in a Taylor vortex flow when the outer cylinder is fixed and the inner cylinder is rotated will be shown.

  The measurement part of FIG. 3 and the apparatus of FIG. 4 were used. The coaxial double cylindrical container is made of an acrylic resin having an outer cylinder radius of 40 mm, an inner cylinder radius of 15 mm, and a height of 75 mm, and the particle locus was photographed using commercially available alumina particles for visualization in an aqueous glycerin solution. The gap between the outer cylinder and the inner cylinder is 25 mm, and the ratio between the gap and the height, that is, the aspect ratio is 3. When the inner cylinder was suddenly accelerated to 100 rpm from a stationary state, a mutant 3-cell mode in which three vortices were generated was generated and continued to rotate at a constant speed for 30 minutes or more. In FIG. 5, the right side is the inner cylinder (rotating part), and the left side is the outer cylinder. The mixed fine particles follow the Taylor vortex and show a uniform vortex-like distribution, but as time passes, the particle group gathers in a certain circular orbit as shown in FIG. That is, the particle accumulation trajectory circulates while maintaining a certain distance without contacting the inner cylinder.

From the above, according to the present embodiment, since it has a flow field of a coaxial double cylindrical container having a small aspect ratio, a flow field mode optimal for the reaction is obtained by collecting fine particles in a liquid in a fixed orbit. It can be formed easily.
In addition, since the group of fine particles in the liquid can be collected on a fixed orbit, an efficient reaction can be performed.
Further, since the container can be made small, the apparatus can be simplified and economical.

  Next, a second embodiment according to the present invention will be specifically described. However, it goes without saying that the present invention is not limited to these examples.

  Here, in a Taylor vortex flow with an aspect ratio of 3, an example of magnetic field control in a flow field mode using ferromagnetic fine particles of magnetic fluid as magnetic fine particles is shown.

  The measurement part of FIG. 3 and the apparatus of FIG. 4 were used. A commercially available water-based magnetic fluid (ferricolloid W-40) was used as the magnetic fine particles. The coaxial double cylindrical container is made of acrylic resin having an outer cylinder radius of 40 mm, an inner cylinder radius of 20 mm, and a height of 60 mm, and a permanent magnet having a height x width x thickness of 60 mm x 30 mm x 30 mm is used for the applied magnetic field. And installed horizontally from the outside of the coaxial double cylindrical container. In order to confirm the internal flow state, the velocity distribution was measured using a commercially available ultrasonic flow velocity distribution meter (UVP X3 PSi). The time change of the obtained velocity distribution is shown in FIG. In FIG. 5, the horizontal axis indicates time, the vertical axis indicates the axial position, and the plot indicates speed values. In Taylor vortex flow with an aspect ratio of 3, several cell modes occur, but in FIG. 5, by applying a magnetic field to the flow field mode in which four cells appear, that is, the normal 4-cell mode, It can be seen that the flow field mode in which the three cells rotate, that is, the change to the mutated three-cell mode. FIG. 6 shows a temporal change in velocity distribution when a magnetic field is applied from a stationary state and the magnetic field is released when the rotation is constant. When a magnetic field is not applied, four flow field modes of normal 2-cell mode, normal 4-cell mode, mutant 3-cell mode, and mutant 4-cell mode are generated stochastically. Only the 3-cell mode can be generated.

  From the above, according to the present embodiment, since it has a coaxial double cylindrical flow with a small aspect ratio and a magnetic field control process of the flow field, the necessary flow field mode is controlled by controlling the application and release of the magnetic field. Can be selected.

In addition, since the coaxial double cylinder has a porous membrane that allows magnetic fine particles or reactants to permeate on the side surfaces of both or one of the cylinders, the reaction solution can be circulated while leaving the magnetic fine particles in the reaction layer. It can be preferably used for a wide range of applications.
Moreover, since the measurement unit and the control unit are separated, the measurement unit can be remotely operated.

  The subject to be measured has been described flow field control and stirring effect using magnetic fine particles of magnetic fluid as magnetic fine particles. It can also be applied to polymerization.

Moreover, the target specimen can be applied not only to fine particles but also to analysis of the behavior of microorganisms such as living bacteria and algae, efficiency of microbial culture, and bioreactor.
It can also be applied to very small devices, contributing to next-generation research that deals with microfluidic and nanofluidic, microbial flow analysis, and microreaction analysis.

  It is explanatory drawing of schematic structure which shows one Example of the rotation reaction apparatus of this invention.   It is explanatory drawing which shows one Example of the rotation reaction method of this invention.   It is explanatory drawing of one Example of the rotation reaction apparatus and rotation reaction method of this invention.   It is explanatory drawing of one Example of the rotation reaction apparatus and rotation reaction method of this invention.   It is a result figure of one Example of the rotation reaction apparatus and rotation reaction method of this invention.   It is a result figure of one Example of the rotation reaction apparatus and rotation reaction method of this invention.   It is a result figure of one Example of the rotation reaction apparatus and rotation reaction method of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Measurement part 2 Control part 3 Coaxial double cylindrical container 4 Reaction fluid 5 Magnetic fine particle 6 Permeation membrane wall 7 Constant temperature apparatus 8 Rotation apparatus 9 Rotation control apparatus 10 Electromagnet 11 Magnetic field control apparatus 12 Circulation apparatus 13 Display apparatus

Claims (2)

A coaxial double-cylindrical container having a height several times the gap between the two cylinders when two cylinders having different diameters are arranged concentrically;
A reaction fluid that fills the gap between the coaxial double cylindrical containers;
Magnetic fine particles mixed in the reaction fluid;
A thermostat for controlling the temperature of the reaction fluid;
A rotating mechanism that rotates both or any one of the cylinders in the coaxial double cylindrical container;
A rotation control device for controlling the rotation of the rotation mechanism;
A permeable membrane wall having a porous membrane that allows the magnetic fine particles or reactants to pass through the side surfaces of both or one of the cylinders in the coaxial double cylindrical container;
A circulation device for flowing the reaction liquid that has permeated through the permeable membrane wall from the other cylindrical wall;
An electromagnet disposed outside the coaxial double cylindrical container and forming a magnetic field in the cylindrical gap;
A magnetic field control device for controlling the magnetic field distribution and magnetic field intensity of the electromagnet;
A rotary reaction device having a display device indicating the indicated values of the magnetic field control device and the rotation control device. A step of installing a coaxial double cylindrical container having a height several times the clearance between both cylinders when two cylinders having different diameters are arranged concentrically;
Filling the reaction fluid into the gap between the coaxial double cylindrical containers;
Mixing magnetic fine particles into the reaction fluid;
Managing the temperature of the reaction fluid;
Rotating both or any one of the cylinders in the coaxial double cylindrical container;
A process for controlling the rotational angular velocity and the rotational speed of the cylinder,
A step of allowing magnetic fine particles or reactants to permeate the side surfaces of both or one of the cylinders in the coaxial double cylindrical container;
Circulating the permeated solution and substance from the other cylindrical wall; and
A step of arranging outside the coaxial double cylindrical container and forming a magnetic field in the cylindrical gap;
Controlling the magnetic field distribution and the magnetic field strength;
A rotational reaction method comprising a step of indicating values of parameters such as magnetic field distribution, magnetic field strength, rotational angular velocity, rotational speed, temperature, and the like.

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