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移動相をその組成を変化させながら送液する送液機構と、分離カラムからの溶出液を先端部より滴下するプローブとを備えた液体分画装置において、
前記プローブは前記送液機構により送液される移動相とは別の第２の移動相を先端部に送る流路を備え、
前記第２の移動相は前記送液機構により送液される移動相の組成を前記プローブ先端部において一定の組成に近づける組成をもったものであることを特徴とする液体分画装置。
In a liquid fractionation apparatus comprising a liquid feeding mechanism for feeding a mobile phase while changing its composition, and a probe for dropping an eluate from a separation column from the tip. The probe includes a flow path for sending a second mobile phase different from the mobile phase fed by the liquid feeding mechanism to the tip, The liquid fractionation apparatus, wherein the second mobile phase has a composition that brings the composition of the mobile phase fed by the liquid feeding mechanism close to a constant composition at the tip of the probe.
前記プローブは、２以上の流路が形成された多重管構造である請求項１に記載の液体分画装置。
The liquid fractionation apparatus according to claim 1, wherein the probe has a multi-tube structure in which two or more flow paths are formed.
前記プローブは３重管構造であり、最内側を分離カラムからの溶出液が流れ、その外側をマトリックス支援レーザ脱離イオン化法による質量分析計で分析を行なうための試料作成用のマトリックス化合物の溶液であるマトリックス液が流れ、最外側を第２の移動相が流れるようになっている請求項１又は２に記載の液体分画装置。
The probe has a triple tube structure, and the eluate from the separation column flows on the innermost side, and the outer side of the probe is a matrix compound solution for sample preparation for analysis with a mass spectrometer based on matrix-assisted laser desorption / ionization. The liquid fractionation apparatus according to claim 1 or 2, wherein the matrix liquid flows and the second mobile phase flows on the outermost side.
前記プローブは３重管構造であり、最内側を分離カラムからの溶出液が流れ、その外側を第２の移動相が流れ、最外側をマトリックス支援レーザ脱離イオン化法による質量分析計で分析を行なうための試料作成用のマトリックス化合物の溶液であるマトリックス液が流れるようになっている請求項１又は２に記載の液体分画装置。
The probe has a triple tube structure, the eluate from the separation column flows on the innermost side, the second mobile phase flows on the outer side, and the outermost side is analyzed by a mass spectrometer based on matrix-assisted laser desorption / ionization. The liquid fractionation device according to claim 1 or 2, wherein a matrix liquid, which is a solution of a matrix compound for sample preparation for flow, flows.
Improved methods and devices for concentration and fractionation of analytes for chemical analysis including matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms)
Pipette tip solid-phase extraction and ultra-performance liquid chromatography/mass spectrometry based rapid analysis of picrosides from Picrorhiza scrophulariiflora